A method of inducing blastocyst formation in pluripotent embryonic stem cells

By combining GSK3 inhibitors, FGF activators, and PDGFr pathway activators, a novel embryonic stem cell culture system was constructed, which solved the problem of low chimerism efficiency of mouse pluripotent embryonic stem cells and achieved highly efficient chimerism of pluripotent embryonic stem cells into the embryo, which can completely replace the function of epiblast cells in the blastocyst.

CN122128220APending Publication Date: 2026-06-02GUANGZHOU NAT LAB
View PDF 1 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
GUANGZHOU NAT LAB
Filing Date
2024-12-02
Publication Date
2026-06-02

AI Technical Summary

Technical Problem

In the current technology, the chimerism efficiency of mouse pluripotent embryonic stem cells is low and they cannot effectively form normal fetuses. The roles of FGF4 and PDGFra signaling pathways in the formation of Epi, PrE and TE in blastocysts have not been fully elucidated.

Method used

A novel embryonic stem cell culture system was constructed by combining GSK3 inhibitors, FGF activators, and PDGFr pathway activators, particularly CHIR-99021, FGF4, and PDGF-AA, with heparin, to induce blastocysts to form pluripotent embryonic stem cells.

Benefits of technology

The study achieved highly efficient chimerism of pluripotent embryonic stem cells into the embryo, with a chimerism efficiency of at least 75%. These cells can completely replace the function of epiblast cells in the blastocyst and do not differentiate into PrE and TE lineages, thus providing a highly efficient pluripotent embryonic stem cell model.

✦ Generated by Eureka AI based on patent content.

Smart Images

  • Figure CN122128220A_ABST
    Figure CN122128220A_ABST
Patent Text Reader

Abstract

This invention discloses a method for inducing blastocysts to form pluripotent embryonic stem cells, thereby constructing a new pluripotent embryonic stem cell line with highly efficient chimeric fetal capacity, which can completely replace the function of epiblast cells in blastocysts. This provides a new model for studying the epigenetic mechanism and transcriptional status of embryonic stem cell pluripotency, and has great application potential for studying mammalian development and generating mouse models.
Need to check novelty before this filing date? Find Prior Art

Citation Information

Patent Citations

  • a CONNECTOR FOR FEEDING ELECTRICAL CURRENT IN A TOOL LOCATED IN A DRILLING OR OIL WELL

    AR014418A1