3D printing bio-ink, composite tissue engineering scaffold and 3D printing method and application thereof

Abstract

This invention discloses a 3D printing bio-ink, a composite tissue engineering scaffold, and their 3D printing methods and applications. The 3D printing bio-ink comprises component A and component B; component A includes 500-1500 g / L β-tricalcium phosphate, 0.5-5 g / L collagen, 0-10 g / L graphene oxide, and 0.1-5 g / L chitosan and acetic acid; component B includes 100-400 g / L cellulose nanocrystals and water; the volume ratio of components A to B is (0.5-2):1. The 3D printing method for the composite tissue engineering scaffold includes the following steps: loading the 3D printing bio-ink into a 3D printer; designing and importing preset scaffold model parameters; setting the printhead specifications according to the ink properties; correcting the printing offset; determining the printing pressure and speed; setting the printhead temperature and printing platform temperature; forming the preset structure using direct-write printing; pre-drying in an oven followed by supercritical drying to obtain the composite tissue engineering scaffold. The composite tissue engineering scaffold of this invention has excellent biocompatibility and structural stability and can be used in the repair of jawbone defects and other oral tissue regeneration fields.

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