Bee feed for artificial breeding and method for preparing the same

Abstract

This application discloses a bumblebee feed for artificial breeding and its preparation method, relating to the field of feed technology. It addresses the problem of unclear dosage of potassium sorbate in bumblebee feed, leading to uncontrollable preservative effects or adverse effects on bumblebees. The feed consists of a feed base and potassium sorbate as a preservative, with a mass percentage of 0.08%–0.12%. The preparation method involves mixing potassium sorbate with the feed base to achieve a final mass percentage of 0.08%–0.12% in the feed. The mixing time is 5–30 minutes, and the mixing temperature is 15–30°C. A mother liquor method is used for sugar water feed, and a premixing method is used for pollen feed. This application experimentally determined a specific safe dosage of 0.1%, which can keep the feed fresh and mold-free for one week under conditions of 28°C and 55% humidity, ensuring safety for bumblebees and providing a directly applicable preservative feed technology solution for commercial bumblebee breeding.

Description

Technical Field

[0001] This invention relates to the field of feed technology, specifically to a bumblebee feed for artificial breeding and its preparation method. Background Technology

[0002] Artificial feed is susceptible to microbial contamination and mold growth during storage and use, leading to feed spoilage, decreased nutritional value, and even the production of mycotoxins harmful to farmed animals. In insect farming, especially commercial bumblebee farming, feed preservation is a key technical aspect for ensuring successful breeding.

[0003] In the field of bumblebee farming, Chinese patent (CN120732100A) discloses a special feed for bumblebee pollination colonies, which mentions adding potassium sorbate as a preservative to the feed. However, the amount of potassium sorbate added in this patent is too large; the amount added in Examples 1 and 2 is about 0.2%, and the amount added in Example 3 is about 0.6%. Moreover, the preservative effect is achieved by potassium sorbate and ε-polylysine together. On the other hand, the feed has many components, which increases the feeding cost.

[0004] Different aquaculture species have significantly different tolerances to preservatives, and excessive amounts of preservatives may have adverse effects on the health and reproduction of bumblebees.

[0005] Therefore, the purpose of this invention is to study a feed with extremely low preservative content, which can ensure the preservative effect of the feed without adversely affecting the health and reproduction of bumblebees. Summary of the Invention

[0006] The purpose of this invention is to provide a bumblebee feed for artificial breeding, its preparation method, and its application, in order to solve the technical problems existing in the prior art: (1) The dosage of potassium sorbate in bumblebee breeding feed in the existing technology is too high; (2) Existing technologies do not provide differentiated preparation methods for different feed bases (liquid sugar water feed, solid pollen feed), which makes it difficult to ensure the uniformity of potassium sorbate dispersion in feed and the anti-corrosion effect is unstable.

[0007] To achieve the above objectives, the present invention adopts the following technical solution: This invention provides a bumblebee feed for artificial breeding, composed of a feed base and potassium sorbate. The potassium sorbate content, based on the mass of the feed base, is 0.08% to 0.12%. This dosage, determined through systematic experimental research, is the optimal dosage, ensuring both effective preservative effects and safety for the bumblebees. By precisely controlling the potassium sorbate content at 0.08% to 0.12%, the growth of microorganisms in the feed can be effectively inhibited, preventing mold growth, without adversely affecting the health and reproduction of the bumblebees.

[0008] The amount of potassium sorbate added is 0.1% based on the mass of the feed base. Furthermore, the feed is for artificial bumblebee breeding. Bumblebees are important pollinating insects and have significant application value in commercial pollination services. The preservative-preserving feed of this invention is particularly suitable for artificial bumblebee breeding and can meet the technical requirements for feed preservation in bumblebee breeding.

[0009] Furthermore, the feed is either sucrose feed or pollen feed. Sucrose feed and pollen feed are the two most commonly used feed types in bumblebee artificial breeding. Sucrose feed mainly provides energy for bumblebees, while pollen feed mainly provides protein and other nutrients. The potassium sorbate preservation solution of the present invention is applicable to these two main feed types.

[0010] This invention also provides a method for preparing bumblebee feed for artificial breeding, comprising the step of mixing potassium sorbate with feed base material to achieve a potassium sorbate content of 0.08%~0.12% by mass in the final feed; the mixing time is 5~30 minutes, and the mixing temperature is 15~30℃. This preparation method ensures that potassium sorbate is uniformly dispersed and maintains stability in the feed by controlling two key process parameters: mixing time and mixing temperature. A mixing time of 5~30 minutes ensures that potassium sorbate and feed base material are thoroughly and uniformly mixed, while a mixing temperature of 15~30℃ ensures the stability of potassium sorbate, avoiding decomposition due to excessively high temperatures or affecting its solubility or dispersion in the feed due to excessively low temperatures.

[0011] Furthermore, when the feed base is sugar water, potassium sorbate is first dissolved in water to prepare a stock solution, and then the stock solution is mixed with the sugar water. This stock solution method is particularly suitable for liquid feed bases. By first dissolving potassium sorbate in water to prepare a high-concentration stock solution, and then mixing the stock solution with the sugar water, it is possible to ensure that potassium sorbate is quickly and evenly dispersed in the sugar water, avoiding the problems of excessively high local concentrations or uneven dispersion caused by directly adding solid potassium sorbate. The concentration of the stock solution can be adjusted according to actual needs, preferably around 10%, which is both easy to operate and ensures good dispersion.

[0012] Furthermore, when the feed base is pollen, potassium sorbate is first premixed with a small amount of pollen, and then mixed evenly with the remaining pollen. This premixing method is particularly suitable for solid feed bases. By premixing potassium sorbate with a small amount of pollen first, the potassium sorbate is evenly adsorbed onto the surface of the pollen particles. Then, the premix is ​​mixed with the remaining large amount of pollen, which effectively avoids the problem of uneven dispersion or agglomeration of potassium sorbate in solid pollen. The amount of pollen used in the premix is ​​usually 1% to 2% of the total pollen, and the premixing time is 5 to 10 minutes, which ensures sufficient dispersion of potassium sorbate.

[0013] This invention also provides a method for artificially raising bumblebees, which involves applying a preservative feed containing 0.08% to 0.12% potassium sorbate to the artificial raising of bumblebees. This method can effectively extend the shelf life of the feed, keeping it fresh and free from mold within a one-week replacement cycle, while having no significant impact on the survival rate and egg-laying rate of the bumblebee queen. This provides a reliable feed preservation technology solution for commercial bumblebee farming.

[0014] Compared with the prior art, the present invention has the following beneficial effects: (1) Through systematic experimental research, this invention has determined that the safe addition dose of potassium sorbate in bumblebee feed is 0.08%~0.12%. The feed is preserved with extremely low preservative addition, ensuring the safety and health of bumblebees. Moreover, the feed composition is simple, reducing breeding costs.

[0015] (2) The addition of 0.08%~0.12% potassium sorbate can effectively inhibit the growth of microorganisms in the feed. Experimental verification shows that under the conditions of 28℃ and 55% humidity, the preservative feed of the present invention remains fresh and free from mold within one week, while ordinary feed without potassium sorbate shows obvious mold after 24 hours. The preservative effect is significantly improved and can meet the actual needs of the one-week replacement cycle in bumblebee breeding.

[0016] (3) Safe and reliable for bumblebees. After 45 days of continuous feeding experiments, the feed containing 0.08%~0.12% potassium sorbate had no significant effect on the survival rate and egg-laying rate of bumblebee queens. The number of egg-laying queens in the experimental group (36) was not significantly different from that in the control group (31), which fully proved the safety of this dosage for bumblebees, ensured the health and reproductive capacity of the farmed animals, and provided a reliable guarantee for commercial bumblebee farming.

[0017] (4) The preparation method provided by this invention is simple to operate and provides preferred preparation methods (mother liquor method and premixing method) for different feed base types (liquid sugar water feed and solid pollen feed), ensuring that potassium sorbate is evenly dispersed in the feed and that the antiseptic effect is stable and reliable. The preparation process does not require special equipment, the process parameters are clear, and it is easy to promote and apply in commercial production, with good practicality and operability. Detailed Implementation

[0018] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as a limitation of the present invention, but rather as a more detailed description of certain aspects, features, and embodiments of the present invention. It should be understood that the terminology used in this invention is merely for describing particular embodiments and is not intended to limit the present invention.

[0019] Furthermore, regarding the numerical ranges in this invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Any stated value or intermediate value within a stated range, as well as each smaller range between any other stated value or intermediate value within said range, are also included within this invention. The upper and lower limits of these smaller ranges may be independently included or excluded from the range.

[0020] Unless otherwise stated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. While only preferred methods and materials have been described herein, any methods and materials similar or equivalent to those described herein may be used in the implementation or testing of this invention. It should be noted that any aspects of this invention not described in detail are conventional practices in the art and are not the focus of this invention.

[0021] The terms “comprising,” “including,” “having,” “containing,” etc., used in this invention are all open-ended terms, meaning that they include but are not limited to.

[0022] This invention discloses a bumblebee feed for artificial breeding, which is composed of feed base and potassium sorbate. The amount of potassium sorbate added is 0.08% to 0.12% based on the mass of the feed base, for example, 0.08%, 0.1% and 0.12%.

[0023] In some preferred embodiments, the amount of potassium sorbate added is 0.1% based on the mass of the feed base. Further, the feed is for artificial bumblebee breeding. Bumblebees are important pollinating insects and have significant application value in commercial pollination services. The preservative-preserving feed of the present invention is particularly suitable for artificial bumblebee breeding and can meet the technical requirements for feed preservation in bumblebee breeding.

[0024] In some preferred embodiments, the feed is a sugar water feed or a pollen feed. Sugar water feed and pollen feed are the two most commonly used feed types in bumblebee artificial breeding. Sugar water feed mainly provides energy for bumblebees, while pollen feed mainly provides protein and other nutrients. The potassium sorbate preservation solution of the present invention is applicable to these two main feed types.

[0025] This invention also provides a method for preparing bumblebee feed for artificial breeding, including a step of mixing potassium sorbate with feed base material to achieve a potassium sorbate content of 0.08%~0.12% by mass in the final feed; the mixing time is 5~30 minutes (e.g., 5 minutes, 30 minutes, or any time value within this range), and the mixing temperature is 15~30℃ (e.g., 15℃, 25℃, 30℃, or any temperature value within this range). This preparation method ensures that potassium sorbate is uniformly dispersed and maintains stability in the feed by controlling the two key process parameters of mixing time and mixing temperature. The stirring process can be carried out using a mixer; the stirring speed for sugar water feed can be selected as 200 rpm; the stirring speed for pollen feed can be selected as 50 rpm, 100 rpm, or any speed between 50 and 100 rpm.

[0026] A mixing time of 5 to 30 minutes ensures that potassium sorbate is fully and evenly mixed with the feed base. A mixing temperature of 15 to 30°C ensures the stability of potassium sorbate and avoids decomposition of potassium sorbate due to excessively high temperature or its solubility or dispersion in feed due to excessively low temperature.

[0027] In some optional embodiments, when the feed base is sugar water, potassium sorbate is first dissolved in water to prepare a stock solution, and then the stock solution is mixed with the sugar water. This stock solution method is particularly suitable for liquid feed bases. By first dissolving potassium sorbate in water to prepare a high-concentration stock solution, and then mixing the stock solution with the sugar water, it is possible to ensure that potassium sorbate is quickly and evenly dispersed in the sugar water, avoiding the problems of excessively high local concentrations or uneven dispersion caused by directly adding solid potassium sorbate. The concentration of the stock solution can be adjusted according to actual needs, preferably around 10%, which is both easy to operate and ensures good dispersion.

[0028] In some alternative embodiments, when the feed base is pollen, potassium sorbate is first premixed with a small amount of pollen, and then mixed evenly with the remaining pollen. This premixing method is particularly suitable for solid feed bases. By premixing potassium sorbate with a small amount of pollen, the potassium sorbate is uniformly adsorbed onto the surface of the pollen particles. Then, the premix is ​​mixed with the remaining large amount of pollen, which effectively avoids the problem of uneven dispersion or agglomeration of potassium sorbate in solid pollen. The amount of pollen used in the premix is ​​usually 1% to 2% of the total pollen, and the premixing time is 5 to 10 minutes, which ensures sufficient dispersion of potassium sorbate.

[0029] This invention also provides the application of bumblebee feed for artificial bumblebee breeding. Specifically, it discloses a method for artificial bumblebee breeding in which a preservative-containing feed containing 0.08%~0.12% potassium sorbate is applied. This effectively extends the shelf life of the feed, keeping it fresh and free from mold within a one-week replacement cycle, while having no significant impact on the survival rate and egg-laying rate of the bumblebee queen. This provides a reliable feed preservation technology solution for commercial bumblebee breeding.

[0030] The specific embodiments of the present invention will be described in detail below. It should be noted that the potassium sorbate mentioned in the following embodiments is food-grade potassium sorbate, which is a white crystalline powder with a purity of not less than 99%; the sugar in the sugar water feed is sucrose, and the sucrose content is expressed as a percentage by mass; the pollen in the pollen feed is natural bee pollen; the mixing time refers to the time required from the start of mixing to uniform mixing; the mixing temperature refers to the temperature range of the feed base during the mixing process; and the mass percentage refers to the percentage of potassium sorbate mass in the total mass of the feed base.

[0031] It should be noted that the technical contents not described in detail in the embodiments and comparative examples of this invention can be implemented using conventional techniques in the field, and are not key points of protection of the invention, and will not be elaborated here.

[0032] Example 1 This embodiment provides a method for preparing a potassium sorbate preservative sucrose feed. 1g of potassium sorbate is dissolved in 10g of distilled water and stirred thoroughly until completely dissolved, preparing a potassium sorbate stock solution with a mass percentage concentration of 10%. 999g of sucrose solution, prepared by mixing sucrose and water in a 1:1 mass ratio (50% sucrose by mass), is taken. The entire potassium sorbate stock solution is added to the 999g of sucrose solution, and the mixture is stirred thoroughly at room temperature for 5 minutes at a stirring speed of 200 rpm to ensure complete and uniform mixing. After mixing, a sucrose feed with a potassium sorbate mass percentage of 0.1% is obtained. The feed is a transparent to slightly yellow liquid, without sediment or off-odor.

[0033] In the preservative-preservative sugar water feed prepared in this embodiment, the mechanism of action of potassium sorbate is as follows: potassium sorbate ionizes in aqueous solution to produce sorbate ions and potassium ions. Sorbitate ions can penetrate the cell membrane of microorganisms and, once inside the cell, interfere with the enzyme system of the microbial cell, particularly inhibiting the activity of dehydrogenases and catalases, thereby blocking the respiratory metabolism and energy production processes of microorganisms, ultimately leading to inhibited microbial growth or death. An addition of 0.1% can form a sufficient concentration of sorbate ions in the feed, effectively inhibiting the growth of common molds, yeasts, and bacteria, while this concentration has no toxic side effects on insects such as bumblebees.

[0034] Example 2 This embodiment provides a method for preparing potassium sorbate preservative pollen feed. Weigh 1g of potassium sorbate, with the same specifications as in Example 1. Weigh 10g of natural bee pollen, which is yellow to brown granular with a particle size ranging from 0.5 to 2mm. Place 1g of potassium sorbate and 10g of pollen in a mixing container and premix at room temperature (approximately 25°C) for 5 minutes, using either manual stirring or a low-speed mixer (100 rpm), to ensure the potassium sorbate powder is evenly adsorbed onto the pollen particles. After premixing, mix the premix (containing 1g of potassium sorbate and 10g of pollen) with 989g of the remaining pollen using a mixer at room temperature (approximately 25°C) for 20 minutes at a speed of 100 rpm. After mixing, a preservative pollen feed with a potassium sorbate content of 0.1% by mass was obtained. The feed was yellow to brown granules, and the potassium sorbate was evenly dispersed on the surface of the pollen granules and in the gaps between the granules, without obvious agglomeration.

[0035] In the preservative pollen feed prepared in this embodiment, the preservative mechanism of potassium sorbate is the same as that in Example 1. Pollen feed contains rich nutrients such as protein, fat, and vitamins, and is more susceptible to microbial contamination during storage. Adding 0.1% potassium sorbate can effectively extend the shelf life of pollen feed without affecting the nutritional value and palatability of the pollen.

[0036] Compare with Example 1 Sugar water feed with a sucrose content of 50% by mass is prepared by mixing sucrose and water in a 1:1 mass ratio, which means that the amount of potassium sorbate added is 0.

[0037] Compare with Example 2 The difference from Example 1 is that the amount of potassium sorbate added is 0.2% (i.e., 2g / kg).

[0038] Compare with Example 3 The difference from Example 1 is that the amount of potassium sorbate added is 0.6% (i.e., 6g / kg).

[0039] Compare with Example 4 The difference from Example 1 is that the amount of potassium sorbate added is 1% (i.e., 10g / kg).

[0040] Compare with Example 5 The difference from Example 1 is that the amount of potassium sorbate added is 2% (i.e., 20g / kg).

[0041] Example 3 This embodiment is used to verify the preservative effect of potassium sorbate-preservative saccharide feed prepared in Examples 1 and 2. 100g samples of the preservative saccharide feed prepared in Example 1 and the ordinary saccharide feed (without potassium sorbate) prepared in Control Examples 1-5 were placed in sterile glass containers of the same size, with the container openings sealed with sterile gauze to allow air circulation. Both sets of samples were placed in a constant temperature and humidity incubator, with the incubation temperature set at 28℃ and the relative humidity at 55%, in a dark environment. The reason for choosing 28℃ and 55% humidity is that these conditions are close to the actual environmental temperature and humidity of bumblebee farming, and are also suitable conditions for microbial growth, which can accelerate the mold growth process of the feed and facilitate the observation of significant differences in preservative effect in a shorter time.

[0042] The feed should be observed for mold every 24 hours. The observation indicators include: (1) the appearance of mold spots on the surface; (2) the change in feed color; and (3) the occurrence of feed odor.

[0043] The observation results are as follows: The mold growth patterns of the ordinary sugar water feeds in Examples 1-5 were basically the same. Taking Example 1 as an example: after 24 hours of cultivation, white flocculent mold spots began to appear on the surface, covering an area of ​​about 10% of the bottom area of ​​the container, accompanied by a slight rancid odor; after 48 hours, the mold spot area expanded to 30%, the color of the mold spot changed from white to grayish-green, and the rancid odor intensified; after 72 hours, the mold spot area exceeded 50%, obvious mold colonies formed on the surface of the feed, and the feed had completely deteriorated. The preservative sugar water feeds prepared in Examples 1-2 showed no mold spots on the surface, no significant color change, and no off-odor during observations at 24 hours, 48 ​​hours, 72 hours, 96 hours, 120 hours, 144 hours (6 days), and 168 hours (7 days) of cultivation, and the feed remained fresh. After 192 hours (8 days) of cultivation, sporadic small white mold spots began to appear on the surface, covering an area of ​​less than 5%, indicating that the feed had begun to show slight mold growth.

[0044] The above experimental results show that the preservative sugar water feed with added 0.1% potassium sorbate can remain fresh and mold-free for one week (168 hours) under conditions of 28℃ and 55% humidity. Compared with ordinary sugar water feed without potassium sorbate (effective shelf life of about 24 hours), the effective shelf life of the preservative sugar water feed is extended to 168 hours, with an extension of (168~24) / 24×100%=600%. The pollen feed in Example 2 is not significantly different from that in Example 1, indicating that the preservative effect of the feeds in Examples 1 and 2 can meet the actual needs of the one-week replacement cycle in bumblebee farming, providing a reliable feed preservation technology solution for commercial bumblebee farming.

[0045] Mechanism analysis of the preservative effect (taking Example 1 as an example): Potassium sorbate ionizes in sugar water to produce sorbate ions. On a mass fraction basis, after complete ionization of 0.1% potassium sorbate, the theoretical mass concentration of sorbate ions is approximately 0.1% × molecular weight ratio (sorbic acid / potassium sorbate = 112 / 150) × degree of ionization (close to 100%) = 0.075%. This concentration of sorbate ions can effectively inhibit the growth and reproduction of common yeasts, molds, and bacteria in sugar water. The sucrose content in the sugar water is as high as 50%, providing a rich carbon source for microorganisms. Under preservative-free conditions, microorganisms grow rapidly, forming visible colonies within 24 hours. After adding potassium sorbate, the sorbate ions inhibit the respiratory metabolism of microorganisms, preventing them from effectively utilizing sugar for growth and reproduction, thereby achieving a preservative effect. The reason why the preservative feed began to show slight mold after a week of cultivation may include: as the storage time is extended, the concentration of a small amount of potassium sorbate decreases due to oxidative degradation or slow reaction with reducing sugars in the sugar water. At the same time, microbial spores that continue to settle in the environment gradually accumulate. When the effective sorbate ion concentration drops below the critical inhibition concentration, the microorganisms begin to grow slowly.

[0046] Example 4 This embodiment was used to verify the safety of potassium sorbate-preserved sugar water feed prepared in Example 1 and Control Examples 1-5 for bumblebees. Bombus terrestris queen bees were selected as the test subjects. Bombus terrestris is the most commonly used bumblebee species in commercial pollination services, making it representative for safety verification. 258 overwintering Bombus terrestris queen bees that recovered at the same time were randomly divided into 6 groups (n=43 / group), with 43 queen bees in each group. All queen bees were healthy individuals 3-5 days after emergence, with a weight range of 0.8-1.2g. The experimental groups were fed the potassium sorbate-preserved sugar water feed prepared in Example 1, while control groups 2-5 were fed the same potassium sorbate-preserved sugar water feed as Control Examples 2-5. Control group 1 was fed ordinary sugar water feed without potassium sorbate (the formula was the same as Control Example 1, but the feed was changed daily to avoid mold growth).

[0047] Rearing conditions: Temperature 26±2℃, relative humidity 60±10%, dark environment. Each queen bee was individually housed in a transparent plastic box (10cm×10cm×8cm), with a food container and nesting material placed inside. Observations were conducted continuously for 45 days, recording the queen bee's survival status, activity level, and egg-laying activity daily. The 45-day observation period was chosen because it covers the complete physiological cycle of the bumblebee queen from emergence to peak egg-laying, allowing for a comprehensive assessment of the impact of potassium sorbate on queen bee survival and reproduction.

[0048] Survival rate calculation method: The survival status of each group of queen bees was recorded daily, including the time and number of deaths. Egg-laying activity of each group of queen bees was also recorded. The collected survival time data was input into a computer and analyzed using a Cox proportional hazards regression model. The model formula is as follows: ln[h(t,

[0049] The survival rate statistics are shown in Table 1.

[0050] Table 1 The observation results are as follows: Regarding survival rate, the survival rate of queen bees in the experimental group was not significantly different from that in control group 1 during the 45-day observation period (P=0.756>0.05, HR=0.851, meeting the candidate dosage condition of no significant difference in egg production rate). This indicates that 0.1% potassium sorbate has no significant effect on the survival rate of bumblebee queen bees. However, the survival rate of queen bees in control groups 2-5 was significantly different from that in control group 1 during the 45-day observation period (P<0.05).

[0051] Regarding egg-laying, 36 surviving queen bees in the experimental group laid eggs, while 31 surviving queen bees in the control group laid eggs. Statistical analysis showed no significant difference in egg-laying rates between the two groups (P>0.05), indicating that 0.1% potassium sorbate had no significant effect on the egg-laying capacity of bumblebee queens. However, the number of eggs laid by queen bees in the control group (groups 2-5) were 3, 2, 1, and 0, respectively (P<0.01, not meeting the criteria).

[0052] Activity status observation: There was no significant difference in the daily activity status (including crawling, body cleaning, feeding, etc.) of the queen bee in the experimental group and the control group, and both showed normal bumblebee behavior patterns.

[0053] The above experimental results fully demonstrate that the preservative sugar water feed containing 0.1% potassium sorbate has no significant effect on the survival rate, egg-laying rate, and daily activity status of bumblebee queens, and this dosage is safe and reliable for bumblebees. Mechanism analysis of safety: Potassium sorbate has extremely low toxicity; its median lethal dose (LD50) in oral rat tests was 4920 mg / kg body weight, which is practically non-toxic. A bumblebee queen consumes approximately 2-3g of feed daily. Based on a potassium sorbate content of 0.1% in the feed, this equates to a daily intake of approximately 2-3mg of potassium sorbate. Assuming a queen's body weight of 1g, this translates to a daily intake of 2-3mg of potassium sorbate per kilogram of body weight, far below the safe dose. Potassium sorbate is primarily metabolized into carbon dioxide and water in insects via the β-oxidation pathway; the metabolites are non-toxic and do not accumulate in the body. Therefore, an addition of 0.1% ensures both preservative effect and complete safety for bumblebees.

[0054] The embodiments described above are merely preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Various modifications and improvements made by those skilled in the art to the technical solutions of the present invention without departing from the spirit of the present invention should fall within the protection scope defined by the claims of the present invention.

Claims

1. A feed for artificially reared bumblebees, characterized in that, It is composed of feed base and potassium sorbate; the amount of potassium sorbate added is 0.08%~0.12% based on the mass of the feed base.

2. The bumblebee feed for artificial breeding according to claim 1, characterized in that, The amount of potassium sorbate added is 0.1% based on the mass of the feed base.

3. The bumblebee feed for artificial breeding according to claim 1, characterized in that, The feed base is feed for artificial breeding of bumblebees.

4. The bumblebee feed for artificial breeding according to claim 3, characterized in that, The feed base is sugar water feed or pollen feed.

5. A method for preparing bumblebee feed for artificial breeding as described in any one of claims 1 to 4, characterized in that, The process includes mixing potassium sorbate with feed base; the mixing time is 5-30 minutes and the mixing temperature is 15-30℃.

6. The preparation method according to claim 4, characterized in that, When the feed base is sugar water, potassium sorbate is first dissolved in water to prepare a mother liquor, and then the mother liquor is mixed with the sugar water.

7. The preparation method according to claim 4, characterized in that, When the feed base is pollen, potassium sorbate is first premixed with a small amount of pollen, and then mixed evenly with the remaining pollen.

8. A method for artificially raising bumblebees, characterized in that, Add 0.08%~0.12% potassium sorbate to the bumblebee feed base.

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