Antibodies to shigella bacterial specific proteins and uses thereof
By preparing and conjugating Shigella-specific monoclonal antibodies C65 and A61, and combining them with the magnetic bead method, the problem of insufficient specificity and sensitivity in the detection of Shigella in existing technologies has been solved, and efficient enrichment and identification of Shigella has been achieved.
CN122325595APending Publication Date: 2026-07-03MEI YI TIAN BIOLOGICAL MEDICINE WUHAN CO LTD
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Patent Information
- Application Number
- CN202411984876.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-31
- Publication Date
- 2026-07-03
AI Technical Summary
Technical Problem
Existing technologies lack highly specific and sensitive methods for detecting Shigella bacteria, especially methods for detecting Shigella bacteria in food that are not fast or effective enough.
Method used
Monoclonal antibodies C65 and A61 were prepared and conjugated with magnetic beads to form magnetic bead antibody conjugates, which were used to enrich Shigella bacteria. Combined with magnetic bead method for sorting and enrichment, specific capture and efficient enrichment of Shigella bacteria were achieved.
Benefits of technology
It achieves high specificity and high sensitivity detection of Shigella bacteria, reduces detection costs, can be used for bacterial identification and culture, and simplifies the detection process.
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Figure CN122325595A_ABST
Abstract
The application discloses a kind of shigella bacteria specific protein antibodies and its application.The antibody is monoclonal antibody C65 and monoclonal antibody A61, monoclonal antibody C65 includes light chain variable region and heavy chain variable region, and the amino acid sequences of light chain variable region and heavy chain variable region are respectively shown as SEQ ID NO:7 and 8;Monoclonal antibody A61 includes light chain variable region and heavy chain variable region, and the amino acid sequences of light chain variable region and heavy chain variable region are respectively shown as SEQ ID NO:9 and 10.The magnetic bead antibody conjugate of the application is coupled by monoclonal antibody C65, monoclonal antibody A61 and activated carboxyl magnetic bead respectively.The magnetic bead antibody conjugate of the application is specific to shigella bacteria, and enrichment efficiency is high, and enrichment operation is simple, can be applied to shigella bacteria metabolite analysis, and also can be used for its qualitative and quantitative analysis.
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