A firming anti-wrinkle composition and method of making and using same

Abstract

The present application relates to the technical field of cosmetic composition, and particularly relates to a firming and anti-wrinkle composition, a preparation method and application thereof, which comprises the following steps: preparing a ternary random copolymer by polymerization, loading tetrahydrocurcumin, and preparing a temperature-sensitive nanoparticle dispersion liquid through dialysis and temperature control refining; hydrating, sterilizing and cooling a humectant, a thickening agent and a chelating agent; sequentially adding an antioxidant active component, a polypeptide and a plant repair component; adjusting pH, compounding a preservative system, adding the temperature-sensitive nanoparticle dispersion liquid, homogenizing, defoaming, filling and obtaining a finished product. The present application realizes stable, slow release of active ingredients through a temperature-sensitive nano-carrier, and synergistically enhances the polypeptide, the lipohydric antioxidant component and the gynostemma pentaphyllum repair component, effectively improves the defects of easy inactivation and poor transdermal absorption of active ingredients, and has excellent multi-functional skin care effects of moisturizing, firming, anti-wrinkle and antioxidant.

Description

Technical Field

[0001] This invention relates to the field of cosmetic composition technology, and more particularly to a firming and anti-wrinkle composition, its preparation method, and its application. Background Technology

[0002] Skin aging is a natural, continuous, and gradual physiological process in the human body. It is also a complex skin pathological process regulated by both endogenous physiological mechanisms and exogenous environmental factors. It mainly manifests as rough, dull skin, decreased elasticity, sagging soft tissue, and the formation of wrinkles. Among these, wrinkle formation and skin laxity are the most core and direct indicators of skin aging. Current research in skin physiology and anti-aging indicates that the core root cause of skin laxity and wrinkles lies in the functional damage to the skin tissue structure and extracellular matrix. The extracellular matrix of the dermis is the core structure that maintains skin firmness, elasticity, and fullness. It is mainly composed of collagen, elastin, hyaluronic acid, and various glycosaminoglycans. Collagen provides mechanical support and strength to the skin, while elastin gives the skin the ability to stretch and recover, together maintaining a smooth and firm state. As the body's metabolic capacity declines, the activity of skin fibroblasts gradually decreases, the synthesis rate of collagen and elastin drops significantly, and the activity of matrix-degrading enzymes increases abnormally, accelerating the decomposition and loss of the skin's core support structure. This leads to insufficient skin support, decreased elasticity, and the gradual formation of laxity and wrinkles. At the level of exogenous factors, ultraviolet radiation, environmental pollution, long-term sleep deprivation, mental stress and external physical stimuli can continuously induce the skin to produce excessive free radicals, triggering oxidative stress and low-grade chronic inflammation, further damaging fibroblast activity, disrupting extracellular matrix homeostasis, accelerating the breakage and disorder of collagen and elastic fibers, and causing a decrease in the skin's ability to retain moisture.

[0003] Currently available firming and anti-wrinkle skincare compositions and related products mainly include plant extract complex systems, peptide active systems, vitamin A active systems, and moisturizing and repairing systems. Among them, plant extract compositions mainly rely on the antioxidant and anti-inflammatory properties of natural plant active ingredients to alleviate skin oxidative damage and help improve skin condition. For example, invention patent CN110897987A discloses an anti-wrinkle and firming skin composition and its application in cosmetics. The main active ingredients include the following components: borage seed oil, betaine, snail secretions, bifida ferment lysate, sea fennel extract, oligopeptide-1, xanthan gum, tocopheryl acetate, fullerene, and triethanolamine. This anti-wrinkle and firming skin composition has a good anti-wrinkle and firming effect.

[0004] However, in existing technologies, natural extracts are mostly combined with common oligopeptides and moisturizing ingredients through simple physical processes, achieving only basic antioxidant and superficial skin repair. They cannot simultaneously target and inhibit dynamic facial expression lines, promote the regeneration of collagen and elastin fibers in the dermis, or improve soft tissue sagging. Their ability to repair deep static wrinkles is also weak. In addition, existing technology systems generally lack targeted delivery and stable protective structures for active ingredients. The peptides and plant active ingredients they contain are mostly water-soluble macromolecules with poor transdermal penetration. They can only remain on the skin surface to provide temporary repair and cannot penetrate deep into the dermis to act on fibroblasts. They cannot fundamentally repair the damaged skin support structure, thus limiting long-term anti-aging and firming effects. Furthermore, heat-sensitive and photosensitive active ingredients are easily degraded and inactivated by light, heat, and acid / alkali environments during storage, resulting in poor shelf-life efficacy stability. Summary of the Invention

[0005] To address the problems mentioned in the background section, the present invention provides a firming and anti-wrinkle composition, its preparation method, and its application.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: A firming and anti-wrinkle composition, based on the total mass of the firming and anti-wrinkle composition, comprises the following phase components in the following mass percentages: Phase A: 5.0-10.0% humectant, 0.25-0.38% thickener, 0.02-0.04% chelating agent; Phase B: Nicotinamide 2.0-4.0%, Ascorbate glucoside 1.0-2.0%, Trehalose 1.5-2.5%, Ergothioneine 0.01-0.03%; Phase C: Acetyl hexapeptide-8 0.004-0.006%, palmitoyl pentapeptide-4 0.0004-0.0006%, palmitoyl tripeptide-1 0.0004-0.0006%, Centella asiatica extract 0.10-0.20%; Phase D: pH adjuster 0.20-0.25%, polyol preservative 0.7-1.1%; Phase E: Temperature-sensitive nanoparticle dispersion 5.0-10.0%; The remainder is deionized water.

[0007] Furthermore, the moisturizers in phase A include small molecule polyols, natural moisturizing factors and polysaccharide moisturizers. The mass percentage of each moisturizer is based on the total mass of the composition, and the total amount of each moisturizer falls within the total proportion range of 5.0-10.0% of the moisturizers in phase A. The small molecule polyol is selected from any one or more of glycerol, 1,3-butanediol, 1,2-hexanediol, and propylene glycol, and the amount added is 2.0-8.0%. The natural moisturizing factor is selected from any one or more of betaine, panthenol, and urea, and the addition amount is 0.5-3.0%; The polysaccharide moisturizer is selected from any one or more of sodium hyaluronate, hydrolyzed sodium hyaluronate, acetylated sodium hyaluronate, and tremella polysaccharide, and the addition amount is 0.05-0.5%.

[0008] Furthermore, the thickener in phase A is selected from one or more of carbomer, xanthan gum, gellan gum, and hydroxyethyl cellulose; The chelating agent is selected from one or more of ethylenediaminetetraacetic acid and its salts, phytic acid and its salts, citric acid and its salts, and gluconic acid and its salts. Preferably, the chelating agent is selected from one or more of disodium ethylenediaminetetraacetate (EDTA-2Na), tetrasodium ethylenediaminetetraacetate (EDTA-4Na), sodium phytate, sodium citrate, and sodium gluconate.

[0009] Furthermore, the pH adjuster in phase D is selected from one or more of arginine, lysine, sodium hydroxide, potassium hydroxide, triethanolamine, and aminomethylpropanol, and is used to adjust the pH value of the composition to a weakly acidic range of 5.5-6.5. The polyol preservative is selected from one or more of 1,2-hexanediol, 1,2-pentanediol, 1,3-propanediol, p-hydroxyacetophenone, octyl glycol, and ethylhexylglycerin.

[0010] Furthermore, the thermosensitive nanoparticle dispersion in phase E is prepared by the following steps: S1. Three monomers, 2-methacryloyloxyethyl phosphorylcholine, N-isopropylacrylamide, and hydroxyethyl methacrylate, are dissolved in a polar organic solvent with 4-cyano-4-(phenylthiocarbamoylthio)valerate and an initiator. The mixture is then subjected to a constant-temperature polymerization reaction under an inert gas atmosphere. After the reaction is completed, the reaction solution is precipitated and purified to obtain a ternary random copolymer. S2. Dissolve the ternary random copolymer and tetrahydrocurcumin together in a water-miscible polar organic solvent and stir until completely dissolved and clear to obtain a polymer-drug co-solution. Place the polymer-drug co-solution into a dialysis bag and dialyze it in deionized water at 4°C with constant temperature stirring to obtain a preliminarily assembled nano-dispersion. S3. The initially assembled nano-dispersion is heated at a constant rate and kept at a constant temperature. Then it is cooled to a low temperature environment, a freeze-drying protectant is added and mixed. After low-temperature freezing and vacuum drying, nano-powder is obtained. After redissolving in pure water, a temperature-sensitive nanoparticle dispersion is obtained.

[0011] Further, in step S1, the mass ratio of 2-methacryloyloxyethyl phosphorylcholine, N-isopropylacrylamide, and hydroxyethyl methacrylate is (23-29):(9-11):1; The initiator is selected from azobisisobutyronitrile or azobisisoheptanenitrile, and its addition amount is 0.5-1.0% of the total mass of 2-methacryloyloxyethyl phosphorylcholine, N-isopropylacrylamide and hydroxyethyl methacrylate; The amount of 4-cyano-4-(phenylthiocarboxylthio)pentanoic acid added is 0.8-1.2% of the total mass of 2-methacryloyloxyethyl phosphorylcholine, N-isopropylacrylamide, and hydroxyethyl methacrylate. The polymerization conditions were as follows: the polar organic solvent was selected from anhydrous ethanol, the total monomer concentration was controlled at 10-15% (w / v), and the reaction was carried out at a constant temperature of 65±2℃ for 10-14h under inert gas protection.

[0012] Furthermore, in step S2, the mass ratio of the ternary random copolymer to tetrahydrocurcumin is (4-6):1; The water-miscible polar organic solvent is selected from one or more of anhydrous ethanol, tetrahydrofuran, and acetone, and the total solid content concentration of the polymer and drug is 2.0-5.0 mg / mL; The conditions for stirring and dissolving are as follows: in a water bath at 25±2℃, magnetically stir at a speed of 300-500 rpm for 30-60 minutes to form a clear and transparent co-solution; The molecular weight cutoff of the dialysis bag is 10-14 kDa, the dialysis temperature is 4±2℃, the external aqueous phase is ultrapure water and is replaced every 2-4 hours, and the total dialysis time is 8-12 hours.

[0013] Furthermore, the final temperature of the uniform heating in step S3 is 37±1℃, and the constant temperature standing time is 1-2h. The temperature to cool down to a low-temperature environment is 4±2℃; The freeze-drying protectant is selected from one or more of D-trehalose, mannitol, and sucrose, and its mass ratio with the total solids in the nano-dispersion is (1-3):1. The conditions for low-temperature freezing are: pre-freezing at (-10)-0℃ for 4-6 hours.

[0014] According to another aspect of the present invention, a method for preparing the above-described firming and anti-wrinkle composition is provided, comprising the following steps: A1. Add the humectant, thickener and chelating agent from phase A to the remaining deionized water in the formula in sequence, stir and hydrate at room temperature for 20-40 minutes until the system is free of particle agglomeration and completely swollen and uniform; then heat to 80-85℃ and keep at a constant temperature for 15-25 minutes for pasteurization, and after sterilization, slowly cool to 55-65℃ for later use to obtain phase A matrix. A2. When the A phase matrix cools down to 55-65℃, add nicotinamide, ascorbate glucoside, trehalose, and ergothioneine from phase B, and stir at a constant temperature for 8-12 minutes until all active components are completely dissolved and dispersed. Cool down to below 40℃ for the second time, and add acetyl hexapeptide-8, palmitoyl pentapeptide-4, palmitoyl tripeptide-1, and centella asiatica extract from phase C in sequence. Stir for 3-5 minutes after each component is added to ensure that each peptide and plant active component is evenly dispersed and to avoid high-temperature inactivation, thus obtaining a mixed system. A3. Cool the mixture to 25-35℃ for the third time, add D-phase pH adjuster to adjust the pH value of the system to a weakly acidic range of 5.5-6.5; add D-phase polyol preservative after pre-stirring and mixing, and continue stirring for 8-15 minutes until the system is homogeneous and transparent. Control the system temperature ≤25℃, and slowly add E-phase thermosensitive nanoparticle dispersion under low-speed stirring at 100-200 rpm in the dark. Continue stirring for 15-25 minutes until the nanoparticles are uniformly dispersed. Avoid high-speed shear, high temperature and strong light environment throughout the process to protect the core-shell structure and temperature response activity of the nanoparticles, and obtain the composite nano system. A4. The composite nano system is degassed under vacuum at a temperature of 20-25℃ and a vacuum degree of (-0.06)-(-0.10) MPa for 8-15 minutes to remove micro-bubbles; aseptic filling is then performed in a low-temperature, clean, and light-protected environment to obtain the finished firming and anti-wrinkle composition.

[0015] According to another aspect of the present invention, the application of the above-mentioned firming and anti-wrinkle composition in skin care products is provided, wherein the firming and anti-wrinkle composition is used to prepare facial or neck skin care products with immediate firming, anti-wrinkle repair, antioxidant and moisturizing effects. The skincare products include serums, lotions, creams, toners, facial masks, skincare creams, and anti-aging repair serums. This composition has good compatibility with common skincare excipients, including emollients, emulsifiers, fragrances, and pigments. After compounding, it can stably retain the firming, anti-wrinkle, antioxidant, and moisturizing effects, and is suitable for facial and neck skin care for dry, oily, and combination skin types.

[0016] The beneficial effects of this invention are: 1. In the technical solution of this invention, the temperature-sensitive nanoparticles exhibit a well-organized core-shell self-assembled structure at the microscopic level. This structure is assembled from a ternary random copolymer as the main carrier and tetrahydrocurcumin as the guest active component through non-covalent interactions. The ternary copolymer molecular chains possess hydrophilic and hydrophobic functional partitioning characteristics. The hydrophobic segments can aggregate inward through intermolecular hydrophobic forces to form a closed core, while the hydrophilic segments extend outward through hydrogen bonding and van der Waals forces to form a hydrophilic shell, which can selectively encapsulate and fix the hydrophobic tetrahydrocurcumin active molecules. This core-shell structure endows the active component with excellent water dispersibility, enabling the hydrophobic active ingredient to be uniformly dispersed in the water-based composition system, effectively improving the problems of active component aggregation and uneven dispersion. Meanwhile, the nanoparticle shell has a biomimetic phospholipid interface structure, which has good interfacial compatibility with the lipid structure of the stratum corneum of human skin. It can effectively weaken the permeability resistance of the skin barrier. Compared with traditional free active ingredients, it can significantly improve the skin penetration efficiency of active ingredients, allowing them to successfully penetrate the stratum corneum and accumulate in the dermis, acting on skin fibroblasts to achieve deep skin repair.

[0017] 2. In the technical solution of this invention, the thermosensitive nano-core-shell structure can effectively physically shield and protect the tetrahydrocurcumin molecules encapsulated within, isolating the active components from direct contact with external light, temperature, oxygen, and metal ions in the system. This effectively inhibits the oxidation, hydrolysis, and isomerization degradation reactions of the active molecules, significantly improving the technical drawbacks of easily inactivated thermosensitive and photosensitive active ingredients, greatly enhancing the overall storage stability of the composition, ensuring consistent efficacy throughout the product's shelf life, and avoiding problems such as efficacy decay and quality fluctuations. Simultaneously, the encapsulating effect of the nano-core-shell can reduce the instantaneous free concentration of highly active components, weakening the direct irritant effect of active ingredients on skin cells, effectively improving the overall mildness and skin tolerance of the formula, making it suitable for all skin types.

[0018] 3. In the technical solution of this invention, a stable formula system is used to achieve efficient compatibility and synergistic effect of peptide repair components, antioxidant components, plant soothing components and nano-loaded active components. The action pathways of each component are complementary and compatible, effectively blocking the skin oxidation and aging process, improving dynamic wrinkles formed by muscle tension, filling static wrinkles formed by dermal layer defects, and lifting loose and sagging soft tissue. It comprehensively improves multiple aging problems such as rough skin, dullness, looseness and wrinkle proliferation, and significantly enhances the overall firming and anti-wrinkle comprehensive performance and skin care application value of the composition. Detailed Implementation

[0019] The technical solution of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0020] In the following examples and comparative examples, the purity of 2-methacryloyloxyethyl phosphorylcholine (CAS: 67881-98-5), N-isopropylacrylamide (CAS: 2210-25-5), hydroxyethyl methacrylate (CAS: 868-77-9), and tetrahydrocurcumin (CAS: 36062-04-1) was ≥98.0%; the purity of azobisisobutyronitrile (CAS: 78-67-1), azobisisoheptanenitrile (CAS: 4419-11-8), 4-cyano-4-(phenylthiocarbamoylthio)valerate (CAS: 201611-92-9), D-trehalose (CAS: 6138-23-4), mannitol (CAS: 87-78-5), sucrose, anhydrous ethanol, tetrahydrofuran, and acetone were all analytical grade, with a purity ≥99.0%.

[0021] Example 1 This embodiment provides a firming and anti-wrinkle composition, which, based on the total mass of the composition, consists of the following components by mass percentage: Phase A: Moisturizer 5.0%: including glycerin 2.0%, betaine 0.5%, sodium hyaluronate 0.5%; carbomer 0.25%, EDTA-2Na 0.02%; Phase B: Nicotinamide 2.0%, Ascorbate glucoside 1.0%, Trehalose 1.5%, Ergothioneine 0.01%; Phase C: Acetyl hexapeptide-8 0.004%, palmitoyl pentapeptide-4 0.0004%, palmitoyl tripeptide-1 0.0004%, Centella asiatica extract 0.10%; Phase D: Arginine 0.20%, adjust system pH to 5.5; 1,2-hexanediol 0.7%; Phase E: 5.0% thermosensitive nanoparticle dispersion; The remainder is deionized water; replenish the system to 100%.

[0022] The preparation method of the temperature-sensitive nanoparticle dispersion in this embodiment includes the following steps: S1. Weigh 23.0g of 2-methacryloyloxyethyl phosphorylcholine, 9.0g of N-isopropylacrylamide, and 1.0g of hydroxyethyl methacrylate, for a total of 33.0g of the three monomers. Accurately weigh 0.165g of the initiator azobisisobutyronitrile and 0.264g of 4-cyano-4-(phenylthiocarbamoylthio)valerate. Add all the above materials to anhydrous ethanol and make up to 330mL of monomer mixed solution (total monomer concentration 10% (w / v)). Purge the system with nitrogen to remove air, and under nitrogen inert gas protection, perform a constant temperature oil bath polymerization reaction at 63℃ with stirring for 10h. After the reaction, precipitate the product with anhydrous diethyl ether, and after multiple purifications and drying, obtain a high-purity ternary random copolymer for later use. S2. Weigh 4.0 g of the ternary random copolymer and 1.0 g of tetrahydrocurcumin prepared above; add the two materials to anhydrous ethanol and make up to 2500 mL of mixed solution, with a total solid content concentration of 2.0 mg / mL; place in a 23℃ constant temperature water bath and magnetically stir at 300 rpm for 30 min until the system is completely clear and transparent; use a dialysis bag with a molecular weight cutoff of 10 kDa to fill the above clear solution and immerse it in 4℃ constant temperature ultrapure water for dialysis for 8 h, replacing the external aqueous phase with fresh ultrapure water every 4 h. After dialysis, a preliminary assembled nano-dispersion is obtained. S3. The initially assembled nano-dispersion is heated to 36℃ at a uniform rate and kept at a constant temperature for 1 hour; then cooled to 2℃ and kept at a low temperature for stabilization; D-trehalose is weighed and mixed according to the ratio of 5.0g of D-trehalose to 5.0g of total solids in the nano-dispersion; the mixed dispersion is pre-frozen at -10℃ for 4 hours, and after low-temperature setting, it is vacuum dried to obtain nano-powder; a certain amount of sterile pure water is used to reconstitute the nano-powder, and after stirring evenly, the finished temperature-sensitive nanoparticle dispersion is obtained.

[0023] The preparation method of the firming and anti-wrinkle composition in this embodiment includes the following steps: A1. Add the humectant, carbomer, and EDTA-2Na from phase A to the remaining deionized water in the formula in sequence. Stir and hydrate at room temperature for 20-40 minutes until the system is free of particle agglomeration and completely swollen and uniform. Then, heat to 80℃ and keep at a constant temperature for 15-25 minutes for pasteurization. After sterilization, slowly cool to 55℃ and set aside for later use to obtain phase A matrix. A2. When the A phase matrix is ​​cooled to 55℃, add nicotinamide, ascorbate glucoside, trehalose, and ergothioneine from phase B, and stir at a constant temperature for 8 minutes until all active components are completely dissolved and dispersed. Cool down to 40℃ for the second time, and add acetyl hexapeptide-8, palmitoyl pentapeptide-4, palmitoyl tripeptide-1, and centella asiatica extract from phase C in sequence. Stir for 3 minutes after each component is added to ensure that each peptide and plant active component is evenly dispersed and to avoid high-temperature inactivation, thus obtaining a mixed system. A3. Cool the mixture to 25°C for the third time, add D-phase arginine to adjust the pH to a weakly acidic range of 5.5; add D-phase 1,2-hexanediol after pre-stirring and mixing, and continue stirring for 8 minutes until the system is homogeneous and transparent. Control the system temperature at 25°C, and slowly add E-phase thermosensitive nanoparticle dispersion under low-speed stirring at 100 rpm in the dark. Continue stirring for 15 minutes until the nanoparticles are uniformly dispersed. Avoid high-speed shearing, high temperature and strong light environment throughout the process to protect the core-shell structure and temperature response activity of the nanoparticles, and obtain the composite nano system. A4. The composite nano system was vacuum degassed for 8 minutes at a temperature of 20℃ and a vacuum degree of (-0.06) MPa to remove micro-bubbles; aseptic filling was completed in a low-temperature, clean, and light-protected environment to obtain the finished firming and anti-wrinkle composition.

[0024] Example 2 This embodiment provides a firming and anti-wrinkle composition, which, based on the total mass of the composition, consists of the following components by mass percentage: Phase A: Humectant 7.5%: including 5.0% of 1,3-butanediol and glycerin in a 1:1 mass ratio, 1.8% of panthenol and betaine in a 1:1 mass ratio, 0.2% of tremella polysaccharide, 0.315% of xanthan gum, and 0.03% of sodium citrate; Phase B: Nicotinamide 3.0%, Ascorbate glucoside 1.5%, Trehalose 2.0%, Ergothioneine 0.02%; Phase C: Acetyl hexapeptide-8 0.005%, palmitoyl pentapeptide-4 0.0005%, palmitoyl tripeptide-1 0.0005%, Centella asiatica extract 0.15%; Phase D: Triethanolamine 0.225%, adjust the pH of the system to 6.0; 1,2-pentanediol and octyl glycol are mixed at a mass ratio of 1:1, 0.9%; Phase E: 7.5% thermosensitive nanoparticle dispersion; The remainder is deionized water; replenish the system to 100%.

[0025] The preparation method of the temperature-sensitive nanoparticle dispersion in this embodiment includes the following steps: S1. Weigh 26.0g of 2-methacryloyloxyethyl phosphorylcholine, 10.0g of N-isopropylacrylamide, and 1.0g of hydroxyethyl methacrylate, for a total of 37.0g of the three monomers. Accurately weigh 0.278g of the initiator azobisisoheptanenitrile and 0.370g of 4-cyano-4-(phenylthiocarbamoylthio)valerate. Add all the above materials to anhydrous ethanol and make up to 296mL of monomer mixed solution (total monomer concentration 12.5% ​​(w / v)). Purge the system with nitrogen to remove air, and under nitrogen inert gas protection, perform a constant temperature oil bath polymerization reaction at 65℃ for 12h with stirring. After the reaction, precipitate the product with anhydrous diethyl ether, and after multiple purifications and drying, obtain a high-purity ternary random copolymer for later use. S2. Weigh 5.0 g of the ternary random copolymer and 1.0 g of tetrahydrocurcumin prepared above; add the two materials to tetrahydrofuran and make up to 1714 mL of mixed solution, with a total solid content concentration of 3.5 mg / mL; place in a 25℃ constant temperature water bath and magnetically stir at 400 rpm for 45 min until the system is completely clear and transparent; use a dialysis bag with a molecular weight cutoff of 12 kDa to fill the above clear solution and immerse it in 4℃ constant temperature ultrapure water for dialysis for 10 h, replacing the external aqueous phase with fresh ultrapure water every 3 h. After dialysis, a preliminary assembled nano-dispersion is obtained. S3. The initially assembled nano-dispersion is heated to 37°C at a uniform rate and kept at a constant temperature for 1.5 hours. Then, it is cooled to 4°C and kept at a low temperature for stabilization. Mannitol freeze-drying protectant is weighed and mixed with 12.0g of mannitol for every 6.0g of total solids in the nano-dispersion. The mixed dispersion is pre-frozen at -5°C for 5 hours. After low-temperature setting, it is vacuum dried to obtain nano-powder. A certain amount of sterile pure water is used to reconstitute the nano-powder. After stirring evenly, the finished temperature-sensitive nanoparticle dispersion is obtained.

[0026] The preparation method of the firming and anti-wrinkle composition in this embodiment includes the following steps: A1. Add the humectant, xanthan gum, and sodium citrate from phase A to the remaining deionized water in the formula in sequence. Stir and hydrate at room temperature for 30 minutes until the system is free of particle agglomeration and completely swollen and uniform. Then, heat to 82℃ and keep at a constant temperature for 15-25 minutes for pasteurization. After sterilization, slowly cool down to 60℃ and set aside to obtain phase A matrix. A2. When the A phase matrix is ​​cooled to 60℃, add nicotinamide, ascorbyl glucoside, trehalose, and ergothioneine from phase B, and stir at a constant temperature for 10 minutes until all active components are completely dissolved and dispersed; cool down to 35℃ for the second time, and add acetyl hexapeptide-8, palmitoyl pentapeptide-4, palmitoyl tripeptide-1, and Centella asiatica extract from phase C in sequence. Stir for 4 minutes after each addition of a component to ensure uniform dispersion of each peptide and plant active component and avoid high-temperature inactivation, thus obtaining a mixed system; A3. Cool the mixed system to 30℃ for the third time, add D-phase triethanolamine to adjust the pH of the system to the weakly acidic range of 6.0; add D-phase 1,2-pentanediol and octyl glycol to the system after pre-stirring and mixing, and continue stirring for 8-15 minutes until the system is homogeneous and transparent. Control the system temperature at 20℃, and slowly add E-phase thermosensitive nanoparticle dispersion under low-speed stirring at 150 rpm in the dark, and continue stirring for 20 minutes until the nanoparticles are uniformly dispersed. Avoid high-speed shear, high temperature and strong light environment throughout the process to protect the core-shell structure and temperature response activity of the nanoparticles, and obtain the composite nano system. A4. The composite nano system was degassed under vacuum at 22℃ and a vacuum of -0.08 MPa for 10 minutes to remove microbubbles. Aseptic filling was then performed in a low-temperature, clean, and light-protected environment to obtain the finished firming and anti-wrinkle composition.

[0027] Example 3 This embodiment provides a firming and anti-wrinkle composition, which, based on the total mass of the composition, consists of the following components by mass percentage: Phase A: Moisturizer 10.0%: including 8.0% of 1,2-hexanediol and propylene glycol in a 1:1 mass ratio, 3.0% of urea and panthenol in a 1:1 mass ratio, 0.5% of acetylated sodium hyaluronate, 0.38% of gellan gum and hydroxyethyl cellulose in a 1:1 mass ratio, and 0.04% of sodium phytate and sodium gluconate in a 1:1 mass ratio. Phase B: Nicotinamide 4.0%, Ascorbate glucoside 2.0%, Trehalose 2.5%, Ergothioneine 0.03%; Phase C: Acetyl hexapeptide-8 0.006%, palmitoyl pentapeptide-4 0.0006%, palmitoyl tripeptide-1 0.0006%, Centella asiatica extract 0.20%; Phase D: Lysine and aminomethylpropanol are mixed at a mass ratio of 1:1 at 0.25%, and the pH of the system is adjusted to 6.5; ethylhexylglycerin and p-hydroxyacetophenone are mixed at a mass ratio of 1:1 at 1.1%. Phase E: 10.0% thermosensitive nanoparticle dispersion; The remainder is deionized water; replenish the system to 100%.

[0028] The preparation method of the temperature-sensitive nanoparticle dispersion in this embodiment includes the following steps: S1. Weigh 29.0g of 2-methacryloyloxyethyl phosphorylcholine, 11.0g of N-isopropylacrylamide, and 1.0g of hydroxyethyl methacrylate, for a total of 41.0g of the three monomers. Accurately weigh 0.410g of the initiator azobisisobutyronitrile and 0.492g of 4-cyano-4-(phenylthiocarbamoylthio)valerate. Add all the above materials to anhydrous ethanol and make up to 273mL of monomer mixed solution (total monomer concentration 15% (w / v)). Purge the system with nitrogen to remove air, and under nitrogen inert gas protection, perform a constant temperature oil bath polymerization reaction at 67℃ for 14h with stirring. After the reaction, precipitate the product with anhydrous diethyl ether, and after multiple purifications and drying, obtain a high-purity ternary random copolymer for later use. S2. Weigh 6.0 g of the ternary random copolymer and 1.0 g of tetrahydrocurcumin prepared above; add the two materials to acetone and make up to 1400 mL of mixed solution, with a total solid content concentration of 5.0 mg / mL; place in a 27℃ constant temperature water bath and magnetically stir at 500 rpm for 60 min until the system is completely clear and transparent; use a dialysis bag with a molecular weight cutoff of 14 kDa to fill the above clear solution and immerse it in 6℃ constant temperature ultrapure water for dialysis for 12 h, replacing the external aqueous phase with fresh ultrapure water every 2 h. After dialysis, a preliminary assembled nano-dispersion is obtained.

[0029] S3. The initially assembled nano-dispersion is heated to 38℃ at a uniform rate and kept at a constant temperature for 2 hours; then cooled to 6℃ and kept at a low temperature for stabilization; sucrose is weighed and mixed according to the ratio of 21.0g of sucrose to 7.0g of total solids in the nano-dispersion; the mixed dispersion is pre-frozen at 0℃ for 6 hours, and after low-temperature setting, it is vacuum dried to obtain nano-powder; a certain amount of sterile pure water is used to reconstitute the nano-powder, and after stirring evenly, the finished temperature-sensitive nanoparticle dispersion is obtained.

[0030] The preparation method of the firming and anti-wrinkle composition in this embodiment includes the following steps: A1. Add the humectant, urea, panthenol, gellan gum, and hydroxyethyl cellulose from phase A to the remaining deionized water in the formula in sequence. Stir and hydrate at room temperature for 40 minutes until the system is free of particle agglomeration and completely swollen and uniform. Then, heat to 85°C and keep at a constant temperature for 25 minutes for pasteurization. After sterilization, slowly cool to 65°C and set aside for later use to obtain phase A matrix. A2. When the A phase matrix is ​​cooled to 65℃, add nicotinamide, ascorbyl glucoside, trehalose, and ergothioneine from phase B, and stir at a constant temperature for 12 minutes until all active components are completely dissolved and dispersed; cool down to 35℃ for the second time, and add acetyl hexapeptide-8, palmitoyl pentapeptide-4, palmitoyl tripeptide-1, and Centella asiatica extract from phase C in sequence. Stir for 5 minutes after each addition to ensure uniform dispersion of each peptide and plant active component and avoid high-temperature inactivation, thus obtaining a mixed system. A3. Cool the mixture to 35°C for the third time, add D-phase lysine and aminomethylpropanol to adjust the pH of the system to a weakly acidic range of 6.5; add D-phase ethylhexylglycerol and p-hydroxyacetophenone to the system after pre-stirring and mixing, and continue stirring for 15 minutes until the system is homogeneous and transparent. Control the system temperature at 20°C, and slowly add E-phase thermosensitive nanoparticle dispersion under low-speed stirring at 200 rpm in the dark, and continue stirring for 25 minutes until the nanoparticles are uniformly dispersed. Avoid high-speed shear, high temperature and strong light environment throughout the process to protect the core-shell structure and temperature response activity of the nanoparticles, and obtain the composite nano system. A4. The composite nano system was degassed under vacuum at 25°C and a vacuum of -0.10 MPa for 15 minutes to remove microbubbles. Aseptic filling was then performed in a low-temperature, clean, and light-protected environment to obtain the finished firming and anti-wrinkle composition.

[0031] Comparative Example 1 The difference between this comparative example and Example 1 is that: no E-phase thermosensitive nanoparticle dispersion is added, and deionized water of the same mass percentage is used instead; the remaining steps are the same as in Example 1.

[0032] Comparative Example 2 The difference between this comparative example and Example 1 is that: the three anti-wrinkle peptides C-phase acetyl hexapeptide-8, palmitoyl pentapeptide-4, and palmitoyl tripeptide-1 are not added, the Centella asiatica extract is retained unchanged, the missing components are replaced with an equal amount of deionized water, and the remaining steps are the same as in Example 1.

[0033] Comparative Example 3 The difference between this comparative example and Example 2 is that ergothionein is not added to phase B, and tetrahydrocurcumin is not loaded during the preparation of phase E nanoparticles. The remaining steps are the same as in Example 2.

[0034] Comparative Example 4 The difference between this comparative example and Example 2 is that the self-made temperature-sensitive nanoparticle dispersion was replaced with an equal amount of ordinary commercial liposome dispersion, while the remaining steps were the same as in Example 2.

[0035] Comparative Example 5 The difference between this comparative example and Example 3 is that: the C-phase Centella Asiatica extract is not added, and deionized water is used as an equal substitute; the remaining steps are the same as in Example 3.

[0036] Comparative Example 6 The difference between this comparative example and Example 3 is that ascorbate glucoside in phase B is removed, and only nicotinamide, trehalose, and ergothioneine are retained. The remaining steps are the same as in Example 3.

[0037] Ninety healthy volunteers, aged 30-55 years (mean age 42.5 ± 5.5 years), with a male-to-female ratio of 1:1, were selected. All volunteers exhibited evenly distributed fine lines, mild skin laxity, and dull, dry skin. Skin types included dry, combination, and oily, with an overall even distribution. Inclusion criteria included: facial wrinkles and laxity meeting the criteria for mild to moderate skin aging; no history of facial cosmetic procedures, skin resurfacing, or chemical peels in the past 3 months; and willingness to cooperate fully with the experiment and adhere to the experimental skincare guidelines. Exclusion criteria included: allergic skin, facial damage, inflammation, or severe acne; use of anti-wrinkle, antioxidant, or whitening skincare products in the past 7 days; pregnancy, breastfeeding, and individuals with abnormal skin function. Volunteers were randomly assigned to 9 groups (corresponding to Examples 1-3 and Comparative Examples 1-6), with 10 participants in each group. There were no statistically significant differences in baseline data on age, skin type, wrinkle severity, and skin hydration among the groups, ensuring data comparability. All participants underwent a uniform 7-day washout period, using only basic, gentle facial cleansers and were prohibited from using any functional skincare products.

[0038] All participants were required to cleanse their faces and sit quietly for 30 minutes to acclimatize to the constant temperature and humidity testing environment before baseline testing. Samples were applied at the same time each morning and evening after cleansing, and no other skincare or makeup products were allowed throughout the testing period. Sun exposure and sauna use were also prohibited. A precise 0.50g sample was applied evenly and completely to a fixed test area on one side of the face, with separate areas for each side. Each test area was 40cm². 2 Use once in the morning and once in the evening for 28 consecutive days; conduct unified indicator tests on day 0, day 7, day 14, and day 28, and take the average value of the test data from 10 people in each group.

[0039] (I) Skin Moisturizing Performance Test: A uniformly calibrated skin stratum corneum moisture meter was used, with a preset test accuracy of ±1%. The test environment was set at a temperature of 22±2℃ and a relative humidity of 50±5%. Before the test, all subjects cleansed their faces with a uniform, gentle facial cleanser to remove facial oil and dirt. They sat naturally for 30 minutes to stabilize their skin condition and establish initial baseline skin moisture data. Three uniform areas on the cheekbone of each subject's face, free of blemishes, acne scars, and dense pores, were marked as permanent test points. 0.50g of each sample was accurately weighed and evenly applied to the fixed test area on one side of the subject's face, with the application area uniformly controlled to 40cm². 2 The skin moisture content was applied thinly and evenly without any accumulation or omissions. After application, the skin was left to stand for 1 hour, 6 hours, and 12 hours. At each time point, the moisture content was measured at fixed test points, with each point tested three times consecutively, and the average value was taken. By comparing the skin moisture content at different durations, the skin hydration retention rate was calculated to quantitatively evaluate the sample's immediate hydration and long-term moisture-locking ability. The calculation formula was: Skin hydration rate (%) = (Skin moisture value at test time / Initial baseline moisture value) × 100%. The results are shown in Table 1. Table 1. Skin moisturizing performance test results of different embodiments and comparative examples

[0040] (II) Skin Firming and Anti-wrinkle Performance Test: A standardized skin elasticity tester and skin surface roughness tester were used. The testing environment was maintained at a constant temperature of 22±2℃, relative humidity of 50±5%, and in a windless, light-protected environment. All subjects underwent a 7-day skin washout period, using only basic facial cleansers and discontinuing all functional skincare products. After cleansing, subjects sat quietly for 30 minutes to stabilize their skin condition. Initial skin elasticity R2 value and average facial fine line roughness R were measured and recorded. a The values ​​were used as baseline data. Ninety subjects were randomly divided into nine groups. Each group received 0.50g of the corresponding sample evenly applied to a fixed test area on the face at fixed times in the morning and evening, with a single application area of ​​40cm². 2 The entire process prohibits sun exposure, high-temperature saunas, and the use of makeup and functional skincare products, simulating a real-life daily skincare scenario. The treatment is repeated for 28 consecutive days to simulate long-term skincare effects. At 7, 14, and 28 days, targeted retests are conducted on the fine lines around the eyes and sagging areas of the cheeks, with each area tested three times. The average value is recorded. Skin elasticity improvement rate and wrinkle reduction rate are calculated. Calculation formulas: Skin elasticity improvement rate (%) = [(28-day R² value - initial R² value) / initial R² value] × 100%; Wrinkle reduction rate (%) = [(initial R² value - initial R² value) / initial R² value] × 100%. a Value -28d R a (value) / initial R a [Value] × 100%. The results are shown in Table 2: Table 2. Skin firming and anti-wrinkle performance test results of different embodiments and comparative examples

[0041] (III) In vitro antioxidant performance test: Prepare a 0.1 mmol / L DPPH anhydrous ethanol standard solution, store it in a light-proof and sealed container for later use, and prepare anhydrous ethanol as a blank solvent. Adjust the UV spectrophotometer, high-speed centrifuge, and constant temperature water bath to standard working conditions. Accurately weigh 2.00 g of each group of test samples, add anhydrous ethanol to make up to 20 mL of sample solution, and use 200 W power ultrasonic extraction for 30 min to fully dissolve the active components in the sample. Then centrifuge at 8000 r / min for 10 min, and take the clear liquid at the top as the test sample solution. Take 2 mL of the test sample solution and 2 mL of DPPH standard solution, mix them thoroughly, and place them in a light-proof test tube. Let them stand at a constant temperature of 25℃ for 30 min to ensure that the free radical reaction is fully balanced. Measure the absorbance A1 of the mixture at the characteristic wavelength of 517 nm. Simultaneously set up a blank control group and a pure solvent control group. Each group of samples was tested in parallel 3 times. The DPPH radical scavenging rate was calculated based on absorbance data to quantitatively evaluate the sample's antioxidant and free radical scavenging capabilities. The calculation formula is: DPPH radical scavenging rate (%) = [1 - (A1 - A2)] / (A2) 空白 [(A solvent)] × 100%. The results are shown in Table 3: Table 3. In vitro antioxidant performance test results of different examples and comparative examples

[0042] As shown in Tables 1, 2, and 3, the long-lasting moisturizing retention rate, 28-day skin elasticity improvement rate, wrinkle improvement rate, and DPPH free radical scavenging rate of Examples 1-3 are significantly better than those of the comparative samples. Furthermore, the overall efficacy exhibits a stable gradient, with Example 2 showing the best overall skincare performance, followed by Examples 3 and 1, which show progressively decreasing effects. This indicates that the firming and anti-wrinkle composition prepared in this invention possesses excellent and balanced multiple skincare benefits, including moisturizing and water-locking, firming and anti-wrinkle, and antioxidant effects. This invention uses self-made temperature-sensitive nanoparticles as a functional carrier, utilizing their temperature-responsive slow-release properties to encapsulate and protect the moisturizing components, compounded anti-wrinkle peptides, and lipid-water dual-phase antioxidant active ingredients. This effectively solves the problems of easy oxidation and inactivation of active ingredients, easy volatilization and loss, and low transdermal absorption efficiency, allowing for continuous and stable release of effective ingredients on the skin surface. Meanwhile, the system constructs a multi-dimensional synergistic skin care system. The compound peptides can target and promote the synthesis of collagen and elastin fibers and inhibit the formation of expression lines. The compound system of tetrahydrocurcumin, ergothioneine and ascorbate glucoside can comprehensively remove free radicals in the aqueous and lipid phases. Combined with Centella Asiatica repair components, it improves the skin barrier structure. The components are adapted to each other at different levels and complement each other in function. Through synergistic effects, the overall skin care performance of the composition, including long-lasting moisturizing, anti-aging and firming, and anti-oxidation, is greatly improved.

[0043] Each comparative example, through the removal of a single variable and the replacement of the carrier, showed varying degrees of reduction in skincare efficacy, with overall performance significantly lower than the example group. Comparative Example 1, lacking a temperature-sensitive nanocarrier, and Comparative Example 4, using ordinary liposomes as a functional carrier, suffered from the absence of nano-encapsulation for stabilization and temperature-controlled slow-release mechanisms. This resulted in ineffective protection of active ingredients, significantly reduced transdermal utilization, and a lack of long-lasting release capabilities, ultimately leading to the most significant reduction in moisturizing duration, anti-wrinkle and firming effects, and antioxidant activity. Comparative Example 2 lacked the core functional components of the compound anti-wrinkle peptides, losing key targets for skin collagen repair and wrinkle reduction, retaining only basic moisturizing capabilities, with almost no firming and anti-wrinkle performance. Comparative Examples 3 and 6 lacked core antioxidant components, disrupting the lipid-water dual-phase antioxidant balance of the system, significantly reducing free radical scavenging ability, and failing to effectively inhibit skin oxidative aging. Comparative Example 5 lacked the Centella Asiatica barrier repair component, resulting in weak skin barrier repair capabilities and impaired basic conditions for skin hydration, anti-aging, and antioxidant effects, indirectly weakening the overall skincare efficacy.

[0044] In summary, this invention utilizes a self-made temperature-sensitive nanoparticle carrier to encapsulate multiple active ingredients, combined with a complex of peptides, lipid-water dual-phase antioxidant components, and centella asiatica repair components to construct a synergistic skincare system. This effectively solves the problems of easy inactivation and poor absorption of active ingredients, enabling the composition to possess excellent and balanced multiple effects of moisturizing, anti-wrinkle, and anti-oxidation.

[0045] In the description of this specification, the reference to terms such as "embodiment," "various embodiments," etc., indicates that a specific feature, structure, material, or characteristic described in connection with that embodiment or preparation example is included in at least one embodiment of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments.

[0046] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.

Claims

1. A firming and anti-wrinkle composition, characterized in that, Based on the total mass of the firming and anti-wrinkle composition, it consists of the following phase components in the following mass percentages: Phase A: 5.0-10.0% humectant, 0.25-0.38% thickener, 0.02-0.04% chelating agent; Phase B: Nicotinamide 2.0-4.0%, Ascorbate glucoside 1.0-2.0%, Trehalose 1.5-2.5%, Ergothioneine 0.01-0.03%; Phase C: Acetyl hexapeptide-8 0.004-0.006%, palmitoyl pentapeptide-4 0.0004-0.0006%, palmitoyl tripeptide-1 0.0004-0.0006%, Centella asiatica extract 0.10-0.20%; Phase D: pH adjuster 0.20-0.25%, polyol preservative 0.7-1.1%; Phase E: Temperature-sensitive nanoparticle dispersion 5.0-10.0%; The remainder is deionized water.

2. The firming and anti-wrinkle composition according to claim 1, characterized in that, The moisturizers in phase A include small molecule polyols, natural moisturizing factors and polysaccharide moisturizers. The mass percentage of each moisturizer is based on the total mass of the composition, and the total amount of each moisturizer falls within the total proportion range of 5.0-10.0% of the moisturizers in phase A. The small molecule polyol is selected from any one or more of glycerol, 1,3-butanediol, 1,2-hexanediol, and propylene glycol, and the amount added is 2.0-8.0%. The natural moisturizing factor is selected from any one or more of betaine, panthenol, and urea, and the addition amount is 0.5-3.0%; The polysaccharide moisturizer is selected from any one or more of sodium hyaluronate, hydrolyzed sodium hyaluronate, acetylated sodium hyaluronate, and tremella polysaccharide, and the addition amount is 0.05-0.5%.

3. The firming and anti-wrinkle composition according to claim 1, characterized in that, The thickener in phase A is selected from one or more of carbomer, xanthan gum, gellan gum, and hydroxyethyl cellulose; The chelating agent is selected from one or more of ethylenediaminetetraacetic acid and its salts, phytic acid and its salts, citric acid and its salts, and gluconic acid and its salts.

4. The firming and anti-wrinkle composition according to claim 1, characterized in that, The pH adjuster in phase D is selected from one or more of arginine, lysine, sodium hydroxide, potassium hydroxide, triethanolamine, and aminomethylpropanol, and is used to adjust the pH of the composition to a weakly acidic range of 5.5-6.

5. The polyol preservative is selected from one or more of 1,2-hexanediol, 1,2-pentanediol, 1,3-propanediol, p-hydroxyacetophenone, octyl glycol, and ethylhexylglycerin.

5. The firming and anti-wrinkle composition according to claim 1, characterized in that, The thermosensitive nanoparticle dispersion in phase E is prepared by the following steps: S1. Three monomers, 2-methacryloyloxyethyl phosphorylcholine, N-isopropylacrylamide, and hydroxyethyl methacrylate, are dissolved in a polar organic solvent with 4-cyano-4-(phenylthiocarbamoylthio)valerate and an initiator. The mixture is then subjected to a constant-temperature polymerization reaction under an inert gas atmosphere. After the reaction is completed, the reaction solution is precipitated and purified to obtain a ternary random copolymer. S2. Dissolve the ternary random copolymer and tetrahydrocurcumin together in a water-miscible polar organic solvent and stir until completely dissolved and clear to obtain a polymer-drug co-solution. Place the polymer-drug co-solution into a dialysis bag and dialyze it in deionized water at 4°C with constant temperature stirring to obtain a preliminarily assembled nano-dispersion. S3. The initially assembled nano-dispersion is heated at a constant rate and kept at a constant temperature. Then it is cooled to a low temperature environment, a freeze-drying protectant is added and mixed. After low-temperature freezing and vacuum drying, nano-powder is obtained. After redissolving in pure water, a temperature-sensitive nanoparticle dispersion is obtained.

6. The firming and anti-wrinkle composition according to claim 5, characterized in that, In step S1, the mass ratio of 2-methacryloyloxyethyl phosphorylcholine, N-isopropylacrylamide, and hydroxyethyl methacrylate is (23-29):(9-11):

1. The initiator is selected from azobisisobutyronitrile or azobisisoheptanenitrile, and its addition amount is 0.5-1.0% of the total mass of 2-methacryloyloxyethyl phosphorylcholine, N-isopropylacrylamide and hydroxyethyl methacrylate; The amount of 4-cyano-4-(phenylthiocarboxylthio)pentanoic acid added is 0.8-1.2% of the total mass of 2-methacryloyloxyethyl phosphorylcholine, N-isopropylacrylamide, and hydroxyethyl methacrylate. The polymerization conditions were as follows: the polar organic solvent was selected from anhydrous ethanol, the total monomer concentration was controlled at 10-15% (w / v), and the reaction was carried out at a constant temperature of 65±2℃ for 10-14h under inert gas protection.

7. The firming and anti-wrinkle composition according to claim 5, characterized in that, In step S2, the mass ratio of the ternary random copolymer to tetrahydrocurcumin is (4-6):1; The water-miscible polar organic solvent is selected from one or more of anhydrous ethanol, tetrahydrofuran, and acetone, and the total solid content concentration of the polymer and drug is 2.0-5.0 mg / mL; The conditions for stirring and dissolving are as follows: in a water bath at 25±2℃, magnetically stir at a speed of 300-500 rpm for 30-60 minutes to form a clear and transparent co-solution; The molecular weight cutoff of the dialysis bag is 10-14 kDa, the dialysis temperature is 4±2℃, the external aqueous phase is ultrapure water and is replaced every 2-4 hours, and the total dialysis time is 8-12 hours.

8. The firming and anti-wrinkle composition according to claim 5, characterized in that, The final temperature of the uniform heating in step S3 is 37±1℃, and the constant temperature standing time is 1-2h. The temperature to cool down to a low-temperature environment is 4±2℃; The freeze-drying protectant is selected from one or more of D-trehalose, mannitol, and sucrose, and its mass ratio with the total solids in the nano-dispersion is (1-3):

1. The conditions for low-temperature freezing are: pre-freezing at (-10)-0℃ for 4-6 hours.

9. A method for preparing a firming and anti-wrinkle composition according to any one of claims 1-8, characterized in that, Includes the following steps: A1. Add the humectant, thickener and chelating agent in phase A to the remaining deionized water in the formula in sequence, stir and hydrate at room temperature, then heat and keep at a constant temperature for pasteurization, and cool down after sterilization to obtain phase A matrix. A2. Add nicotinamide, ascorbyl glucoside, trehalose and ergothioneine from phase B into phase A matrix, and stir at constant temperature until completely dissolved and dispersed; cool down for the second time, and add acetyl hexapeptide-8, palmitoyl pentapeptide-4, palmitoyl tripeptide-1 and centella asiatica extract from phase C in sequence. Stir for 3-5 minutes after each addition to obtain a mixed system. A3. Cool the mixed system for the third time, add phase D pH adjuster to adjust the pH value of the system to 5.5-6.5, add polyol preservative, and continue stirring until the system is homogeneous and transparent. Under light-proof stirring conditions, add phase E thermosensitive nanoparticle dispersion, and continue stirring until uniformly dispersed to obtain composite nano system. A4. The composite nano system is degassed under vacuum and aseptically filled in a low-temperature, clean, and light-protected environment to obtain the finished firming and anti-wrinkle composition.

10. The use of a firming and anti-wrinkle composition as described in any one of claims 1-8 in a skin care product, characterized in that, The firming and anti-wrinkle composition is used to prepare facial or neck skin care products with immediate firming, anti-wrinkle repair, antioxidant and moisturizing effects.

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