Magnetic bead bearing disc sealing imaging mechanism

Abstract

The utility model relates to the technical field of fluorescence immunoassay equipment, in particular to a magnetic bead bearing plate sealing imaging mechanism which comprises a mounting bottom plate, a bearing mechanism and an imaging mechanism, the bearing mechanism comprises a bearing disc arranged on the mounting bottom plate, a bearing groove formed in the bearing disc, a shaft hole formed in the center of the bearing groove, a rotating shaft arranged in the shaft hole and a rotating motor arranged at the lower end of the bearing disc, the upper end of the rotating shaft penetrates through the shaft hole and extends upwards, and the lower end of the rotating shaft is in transmission connection with the rotating motor; the imaging mechanism comprises a supporting frame arranged at the lower end of the mounting bottom plate and a fluorescence microscope arranged on the supporting frame, an observation hole is formed in the bearing groove, and the lens end of the fluorescence microscope corresponds to the observation hole. The design is simple in structure, low in cost and stable in operation.

Description

Technical Field

[0001] This utility model relates to the field of fluorescence immunoassay equipment technology, specifically to a magnetic bead carrier disk sealed imaging mechanism. Background Technology

[0002] Fluorescence detection utilizes the fact that certain compounds, when excited by ultraviolet light, emit light with a wavelength longer than the excitation light. This phenomenon is called fluorescence. Fluorescence detection requires a fluorescence microscope, which combines an optical microscope with the emission of fluorescent dyes from the compound through an excitation light source—a microscopic observation mode. After mixing and incubating magnetic beads, reagents, and samples, observation and imaging are performed using a fluorescence microscope to achieve fluorescence detection. Automated fluorescence immunoassay analyzers, designed to meet the demands of automation and high detection efficiency, incorporate fluorescence microscope imaging mechanisms. However, these mechanisms are complex, bulky, costly, and require cumbersome assembly and manufacturing processes. Utility Model Content

[0003] To solve the above problems, this utility model provides a magnetic bead carrier disk sealing imaging mechanism, which has a simple structure, low cost, and stable operation.

[0004] The technical solution adopted by this utility model is to provide a magnetic bead support disk sealing imaging mechanism, including a mounting base plate, a support mechanism and an imaging mechanism disposed on the mounting base plate. The support mechanism includes a support disk disposed on the mounting base plate, a support groove disposed on the support disk, a shaft hole disposed in the center of the support groove, a rotating shaft disposed in the shaft hole, and a rotary motor disposed at the lower end of the support disk. The upper end of the rotating shaft extends upward through the shaft hole, and the lower end of the rotating shaft is connected to the rotary motor for transmission. The imaging mechanism includes a support frame disposed at the lower end of the mounting base plate and a fluorescence microscope disposed on the support frame. An observation hole is provided in the support groove, and the lens end of the fluorescence microscope corresponds to the observation hole.

[0005] The bearing groove is also provided with a support pad and a sleeve hole provided on the support pad. The support pad is fitted onto the rotating shaft and located in the bearing groove by means of the sleeve hole.

[0006] The support frame is equipped with an adjustment mechanism, which includes an adjustment slide rail on the support frame, an adjustment slider slidably mounted on the adjustment slide rail, an adjustment motor on the support frame, an adjustment lead screw rotatably mounted on the support frame, and an adjustment slide block fitted on the adjustment lead screw. The inner ends of the adjustment slider and the adjustment slide block are fixed, and the outer ends of the fluorescence microscope and the adjustment slide block are fixed. The adjustment motor is connected to the adjustment lead screw via a transmission. The rotation of the adjustment lead screw drives the adjustment slide block to move up and down on the adjustment lead screw. The adjustment slide block drives the fluorescence microscope and the adjustment slider to move synchronously.

[0007] The mounting base plate is also provided with a pressure plate mechanism, which includes a pressure plate frame mounted on the mounting base plate and located on one side of the bearing plate, a pressure plate motor mounted on the pressure plate frame, a pressure plate screw mounted on the pressure plate frame, a pressure plate moving seat mounted on the pressure plate screw, and a pressure block hinged to the outer end of the pressure plate moving seat. One end of the pressure block is hinged to the pressure plate moving seat, and the other end extends into the bearing groove. The pressure plate motor is driven to one end of the pressure plate screw. The rotation of the pressure plate screw drives the pressure plate moving seat to move towards the bearing groove.

[0008] The pressure plate mechanism also includes a pressure plate slide rail mounted on the pressure plate frame and a pressure plate slide block slidably mounted on the pressure plate slide rail. The pressure plate slide rail is parallel to the translation trajectory of the pressure plate moving seat, and the lower end of the pressure plate moving seat and the upper end of the pressure plate slide block are fixed.

[0009] The pressure plate mechanism also includes a guide seat disposed between the pressure plate moving seat and the bearing plate, a clearance opening disposed on the upper end of the guide seat, a guide groove disposed on the end of the guide seat facing the pressure plate moving seat, and guide rods disposed on both sides of the pressure block. The guide rods correspond to the guide grooves, the opening end of the guide grooves faces the pressure plate moving seat, and the horizontal height of the opening end of the guide grooves is lower than the horizontal height of the bottom of the groove.

[0010] The beneficial effects of this invention are that it provides a sealed imaging mechanism for a magnetic bead carrier plate. The magnetic bead sample plate is placed in a carrier groove, and a rotating shaft is inserted into a through hole in the center of the magnetic bead sample plate. A rotary motor drives the rotating shaft to rotate, causing the magnetic bead sample plate to rotate synchronously. A mixture of multiple samples, reagents, and magnetic beads is injected into the magnetic bead sample plate. A fluorescence microscope can detect the luminescence of the magnetic bead sample plate through an observation hole in the carrier groove. This design is simple in structure, low in cost, and meets automation requirements. Attached Figure Description

[0011] Figure 1 This is a schematic diagram of the structure of this utility model;

[0012] Figure 2 This is a structural diagram of the adjusting distance mechanism and the pressure plate mechanism;

[0013] Figure 3 yes Figure 2 Enlarged view of A in the middle;

[0014] Figure 4 yes Figure 2 A magnified view of B in the middle.

[0015] In the attached diagram, 1 is the mounting base plate, 2 is the bearing plate, 3 is the bearing groove, 4 is the shaft hole, 5 is the rotating shaft, 6 is the rotary motor, 7 is the support frame, 8 is the fluorescence microscope, 9 is the observation hole, 10 is the support pad, 11 is the adjustable sliding rail, 12 is the adjustable slider, 13 is the adjustable motor, 14 is the adjustable lead screw, 15 is the adjustable slide block, 16 is the pressure plate frame, 17 is the pressure plate motor, 18 is the pressure plate lead screw, 19 is the pressure plate moving seat, 20 is the pressure block, 21 is the pressure plate sliding rail, 22 is the pressure plate slide block, 23 is the guide seat, 24 is the clearance opening, 25 is the guide through groove, and 26 is the guide rod. Detailed Implementation

[0016] like Figure 1-4 As shown, this utility model provides a magnetic bead carrier disk sealing imaging mechanism, including a mounting base plate 1, a carrier mechanism and an imaging mechanism disposed on the mounting base plate 1. The carrier mechanism includes a carrier disk 2 disposed on the mounting base plate 1, a carrier groove 3 disposed on the carrier disk 2, a shaft hole 4 disposed at the center of the carrier groove 3, a rotating shaft 5 disposed in the shaft hole 4, and a rotary motor 6 disposed at the lower end of the carrier disk 2. The upper end of the rotating shaft 5 extends upward through the shaft hole 4, and the lower end of the rotating shaft 5 is connected to the rotary motor 6 for transmission. The imaging mechanism includes a support frame 7 disposed at the lower end of the mounting base plate 1 and a fluorescence microscope 8 disposed on the support frame 7. An observation hole 9 is disposed in the carrier groove 3, and the lens end of the fluorescence microscope 8 corresponds to the observation hole 9.

[0017] The magnetic bead sample tray is a circular structure with a through hole in the center. In the fluorescence immunoassay analyzer, the magnetic bead sample tray can be transferred from the storage compartment to the carrier groove 3 of the carrier tray 2 via a transfer mechanism. The rotating shaft 5 is inserted into the through hole in the center of the magnetic bead sample tray, and the rotary motor 6 can drive the rotating shaft 5 to rotate, causing the magnetic bead sample tray to rotate synchronously. The magnetic bead sample tray is filled with a mixture of multiple sets of samples, reagents, and magnetic beads. The fluorescence microscope 8 can perform luminescence detection of the magnetic bead sample tray through the observation hole 9 in the carrier groove 3. This design has a simple structure, low cost, and meets the requirements of automation.

[0018] like Figure 2-3 As shown, the bearing groove 3 is also provided with a support pad 10 and a sleeve hole provided on the support pad 10. The support pad 10 is fitted onto the rotating shaft 5 through the sleeve hole and is located in the bearing groove 3.

[0019] When the magnetic bead sample disk is mounted on the rotating shaft 5, the magnetic bead sample disk rubs against the bottom of the bearing groove 3 when it rotates, resulting in high resistance. Therefore, a support pad 10 is added to the rotating shaft 5. The support pad 10 can be placed between the magnetic bead sample disk and the bottom of the bearing groove 3, which greatly reduces the rotational resistance of the magnetic bead sample disk, making the rotation more stable and smoother.

[0020] like Figure 2-3As shown, the support frame 7 is equipped with an adjustment mechanism, which includes an adjustment slide rail 11 on the support frame 7, an adjustment slider 12 slidably mounted on the adjustment slide rail 11, an adjustment motor 13 on the support frame 7, an adjustment lead screw 14 rotatably mounted on the support frame 7, and an adjustment slide block 15 fitted on the adjustment lead screw 14. The inner ends of the adjustment slider 12 and the adjustment slide block 15 are fixed, and the outer ends of the fluorescence microscope 8 and the adjustment slide block 15 are fixed. The adjustment motor 13 is connected to the adjustment lead screw 14 for transmission. The rotation of the adjustment lead screw 14 drives the adjustment slide block 15 to move up and down on the adjustment lead screw 14. The adjustment slide block 15 drives the fluorescence microscope 8 and the adjustment slider 12 to move synchronously.

[0021] The pitch motor 13 drives the pitch screw 14 to rotate on the support frame 7. The pitch slide 15, which is adapted to the pitch screw 14, will rise and fall with the rotation of the pitch screw 14, and the fluorescence microscope 8 fixed to the pitch slide 15 will rise and fall synchronously.

[0022] The distance adjustment mechanism can adjust the distance between the fluorescence microscope 8 and the carrier plate 2, that is, the distance between the microscope and the magnetic bead sample plate, so as to adjust the imaging focal length. It has a simple and practical structure, is easy to control, and improves the detection accuracy.

[0023] like Figure 2 and Figure 4 As shown, a pressure plate mechanism is also provided on the mounting base plate 1. The pressure plate mechanism includes a pressure plate frame 16 located on the mounting base plate 1 and on one side of the bearing plate 2, a pressure plate motor 17 located on the pressure plate frame 16, a pressure plate screw 18 rotatably located on the pressure plate frame 16, a pressure plate moving seat 19 located on the pressure plate screw 18, and a pressure block 20 hinged to the outer end of the pressure plate moving seat 19. One end of the pressure block 20 is hinged to the pressure plate moving seat 19, and the other end extends into the bearing groove 3. The pressure plate motor 17 is drivenly connected to one end of the pressure plate screw 18. The rotation of the pressure plate screw 18 drives the pressure plate moving seat 19 to move towards the bearing groove 3.

[0024] The pressure plate motor 17 drives the pressure plate screw 18 to rotate on the pressure plate frame 16. The pressure plate moving seat 19, which is adapted to the pressure plate screw 18, will translate with the rotation of the pressure plate screw 18, pushing the pressure block 20 at the front end of the pressure plate moving seat 19 into the bearing groove 3. Since the pressure block 20 and the pressure plate moving seat 19 are hinged, when the front and middle parts of the pressure block 20 have entered the bearing groove 3, the front end of the pressure block 20 will fall down due to its own center of gravity and press on the magnetic bead sample plate to achieve the purpose of stabilizing the magnetic bead sample plate.

[0025] The pressure plate mechanism can provide downward pressure on the magnetic bead sample disk in the bearing groove 3, which can prevent the magnetic bead sample disk from shifting after being subjected to force in the bearing groove 3, resulting in unstable rotation and changes in the distance from the fluorescence microscope 8.

[0026] like Figure 2 and Figure 4 As shown, the pressure plate mechanism also includes a pressure plate slide rail 21 disposed on the pressure plate frame 16 and a pressure plate slide seat 22 slidably disposed on the pressure plate slide rail 21. The pressure plate slide rail 21 is parallel to the translation trajectory of the pressure plate moving seat 19, and the lower end of the pressure plate moving seat 19 and the upper end of the pressure plate slide seat 22 are fixed.

[0027] When the pressure plate moving seat 19 moves horizontally, it drives the pressure plate slide seat 22 to move horizontally on the pressure plate slide rail 21. The pressure plate slide seat 22 and the pressure plate slide rail 21 can support and guide the pressure plate moving seat 19, making the movement of the pressure plate moving seat 19 more stable and precise.

[0028] like Figure 2 and Figure 4 As shown, the pressure plate mechanism also includes a guide seat 23 disposed between the pressure plate moving seat 19 and the bearing plate 2, a clearance opening 24 disposed on the upper end of the guide seat 23, a guide groove 25 disposed on the end of the guide seat 23 facing the pressure plate moving seat 19, and guide rods 26 disposed on both sides of the pressure block 20. The guide rods 26 and the guide grooves 25 correspond to each other. The opening end of the guide groove 25 faces the pressure plate moving seat 19, and the horizontal height of the opening end of the guide groove 25 is lower than the horizontal height of the bottom of the groove.

[0029] The guide seat 23 has a U-shaped structure, and the pressure block 20 can pass through the opening at the top of the guide seat 23. The guide groove 25 and the guide rod 26 are set accordingly. When the pressure plate moving seat 19 pushes the pressure block 20 through the opening at the top of the guide seat 23 and into the bearing groove 3, the guide rods 26 on both sides of the pressure block 20 simultaneously enter the guide groove 25. The guide rods 26 are located on both sides of the rear end of the pressure block 20. The guide groove 25 guides the guide rods 26, gradually raising the rear end of the pressure block 20 so that the pressure block 20 can press down stably, ensuring that the pressure block 20 can stably press the magnetic bead sample plate.

Claims

1. A magnetic bead carrier disk sealing imaging mechanism, comprising a mounting base plate (1), a carrier mechanism and an imaging mechanism disposed on the mounting base plate (1), characterized in that: The supporting mechanism includes a supporting plate (2) on the mounting base plate (1), a supporting groove (3) on the supporting plate (2), a shaft hole (4) in the center of the supporting groove (3), a rotating shaft (5) in the shaft hole (4), and a rotary motor (6) at the lower end of the supporting plate (2). The upper end of the rotating shaft (5) extends upward through the shaft hole (4), and the lower end of the rotating shaft (5) is connected to the rotary motor (6) for transmission. The imaging mechanism includes a support frame (7) at the lower end of the mounting base plate (1) and a fluorescence microscope (8) on the support frame (7). An observation hole (9) is provided in the supporting groove (3), and the lens end of the fluorescence microscope (8) corresponds to the observation hole (9).

2. The magnetic bead carrier disk sealing imaging mechanism according to claim 1, characterized in that: The bearing groove (3) is also provided with a support pad (10) and a sleeve hole provided on the support pad (10). The support pad (10) is fitted onto the rotating shaft (5) by means of the sleeve hole and is located in the bearing groove (3).

3. The magnetic bead carrier disk sealing imaging mechanism according to claim 2, characterized in that: The support frame (7) is provided with a distance adjustment mechanism, which includes a distance adjustment slide rail (11) on the support frame (7), a distance adjustment slider (12) slidably disposed on the distance adjustment slide rail (11), a distance adjustment motor (13) on the support frame (7), a distance adjustment lead screw (14) rotatably disposed on the support frame (7), and a distance adjustment slide seat (15) fitted on the distance adjustment lead screw (14). The inner ends of the distance adjustment slider (12) and the distance adjustment slide seat (15) are fixed, and the outer ends of the fluorescence microscope (8) and the distance adjustment slide seat (15) are fixed. The distance adjustment motor (13) is connected to the distance adjustment lead screw (14) for transmission. The rotation of the distance adjustment lead screw (14) drives the distance adjustment slide seat (15) to move up and down on the distance adjustment lead screw (14). The distance adjustment slide seat (15) drives the fluorescence microscope (8) and the distance adjustment slider (12) to move synchronously.

4. The magnetic bead carrier disk sealing imaging mechanism according to claim 1, characterized in that: The mounting base plate (1) is also provided with a pressure plate mechanism. The pressure plate mechanism includes a pressure plate frame (16) set on the mounting base plate (1) and located on one side of the bearing plate (2), a pressure plate motor (17) set on the pressure plate frame (16), a pressure plate screw (18) rotatably set on the pressure plate frame (16), a pressure plate moving seat (19) set on the pressure plate screw (18), and a pressure block (20) hinged to the outer end of the pressure plate moving seat (19). One end of the pressure block (20) is hinged to the pressure plate moving seat (19), and the other end extends into the bearing groove (3). The pressure plate motor (17) is connected to one end of the pressure plate screw (18) through transmission. The rotation of the pressure plate screw (18) drives the pressure plate moving seat (19) to move towards the bearing groove (3).

5. The magnetic bead carrier disk sealing imaging mechanism according to claim 4, characterized in that: The pressure plate mechanism also includes a pressure plate slide rail (21) disposed on the pressure plate frame (16) and a pressure plate slide seat (22) slidably disposed on the pressure plate slide rail (21). The pressure plate slide rail (21) is parallel to the translation trajectory of the pressure plate moving seat (19). The lower end of the pressure plate moving seat (19) and the upper end of the pressure plate slide seat (22) are fixed.

6. The magnetic bead carrier disk sealing imaging mechanism according to claim 4, characterized in that: The pressure plate mechanism also includes a guide seat (23) disposed between the pressure plate moving seat (19) and the bearing plate (2), a clearance opening (24) disposed on the upper end of the guide seat (23), a guide groove (25) disposed on the end of the guide seat (23) facing the pressure plate moving seat (19), and guide rods (26) disposed on both sides of the pressure block (20). The guide rods (26) and the guide grooves (25) correspond to each other. The opening end of the guide grooves (25) faces the pressure plate moving seat (19), and the horizontal height of the opening end of the guide grooves (25) is lower than the horizontal height of the bottom of the groove.

Images