New use of hibiscus 'titanbicus'

Abstract

To provide a new use of Hibiscus 'Titanbicus'.SOLUTION: An anti-anxiety agent and an angiotensin-converting enzyme inhibitor contain Hibiscus 'Titanbicus' containing hyperoside and isoquercitrin at a ratio of (1:3) to (2:1), for example, a flower (including bud) or its extract of Hibiscus 'Titanbicus' peach white (kind registration by the Ministry of Agriculture, Forestry and Fisheries No. 21813), and contain a Hibiscus 'Titanbicus' perennial plant, as the flower (including bud) or its extract of Hibiscus 'Titanbicus', wherein a total amount of the hyperoside and the isoquercitrin contained in the Hibiscus 'Titanbicus' perennial plant is 1.0 mass% or more.SELECTED DRAWING: Figure 1

Description

[Technical field]

[0001] The present invention relates to a novel use of a particular variety of titan bicus. [Background technology]

[0002] Titan bicus is a perennial plant of the Hibiscus genus of the Malvaceae family, and is a new plant developed by the present applicant. Titan bicus is very hardy and extremely resistant to the summer heat. The above-ground parts die in autumn, but because it is a perennial plant, it sprouts the following year and blooms in early summer. The flowering season is mainly between July and September, and each flower lasts for only one day, but a total of nearly 200 flowers bloom in one summer. The flowers are very large, with a diameter of about 15 cm to 25 cm, and the color of the flower varies depending on the variety, and includes red, pink, and white. In addition, depending on the variety, the plant height can reach up to 3 m.

[0003] Known varieties of Titan Bicus include Titan Leia (Ministry of Agriculture, Forestry and Fisheries Variety Registration No. 27541), Titan Flare (Ministry of Agriculture, Forestry and Fisheries Variety Registration No. 28201), Titan Pink (Ministry of Agriculture, Forestry and Fisheries Variety Registration No. 21812), Titan Peach White (Ministry of Agriculture, Forestry and Fisheries Variety Registration No. 21813), and Titan Elf (Ministry of Agriculture, Forestry and Fisheries Variety Registration No. 27540).

[0004] Titanobicus is popular for horticultural and ornamental purposes because it grows vigorously, is easy to cultivate, and produces huge flowers.

[0005] On the other hand, the buds and flowers of Titan bicus can be used for food, and in recent years, they have also been produced as edible flowers. The Titan leia variety has been put to practical use as an edible flower. Titan leia has large flowers, making it an edible flower with a large impact, making it easy to differentiate from other edible flowers, and it can maintain its beauty even when artificially bloomed.

[0006] Recently, various functions of natural plant extracts have been reported (see, for example, Patent Document 1). Such extracts are also widely used as additives for foods, beverages, and cosmetics. For example, Patent Document 1 claims that the use of olive leaf extract containing oleuropein can improve decreased activity and anxiety.

[0007] To date, there have been few reports on the components and physiological activities contained in Titan bicus, and no research has been conducted focusing on varieties, etc. [Prior art documents] [Patent documents]

[0008] [Patent Document 1] JP 2016-132657 A Summary of the Invention [Problem to be solved by the invention]

[0009] The present invention aims to provide new uses for titan bicus by discovering new physiological activities of titan bicus. [Means for solving the problem]

[0010] The anxiolytic agent of the present invention is characterized by containing a flower (including buds) of titan bicus or an extract thereof, which contains hyperoside and isoquercitrin in a ratio of (1:3) to (2:1). In the present invention, the term "flower (including buds) of titan bicus" is a concept that includes not only open flowers but also unopened flowers (buds), and does not limit the content of actual buds. In addition, "flower (including buds) of titan bicus" also includes processed products thereof (e.g., titan bicus powder); the same applies below.

[0011] The product is characterized in that the titan bicus flower (including buds) or extract thereof contains titan bicus powder, and the total amount of the hyperoside and the isoquercitrin contained in the titan bicus powder is 1.0 mass% or more relative to the total mass of the titan bicus powder.

[0012] The anti-anxiety agent of the present invention is characterized by containing the flowers (including buds) of Titan Peach White (Ministry of Agriculture, Forestry and Fisheries Variety Registration No. 21813) or an extract thereof.

[0013] The angiotensin-converting enzyme inhibitor of the present invention is characterized by containing titanbicus flowers (including buds) or an extract thereof, which contain hyperoside and isoquercitrin in a ratio of (1:3) to (2:1).

[0014] The angiotensin-converting enzyme inhibitor of the present invention is characterized by containing the flowers (including buds) of Titan Peach White (Ministry of Agriculture, Forestry and Fisheries Variety Registration No. 21813) or an extract thereof.

[0015] The supplement of the present invention is characterized by containing powder of Titan Peach White (Ministry of Agriculture, Forestry and Fisheries Variety Registration No. 21813) flowers (including buds) or powder of Titan Pleiades flowers (including buds).

[0016] The AGE production inhibitor of the present invention is characterized by containing Titan Pleiades flowers (including buds) or an extract thereof.

[0017] The radical scavenger of the present invention is characterized by containing the flower (including buds) of Titan Pleiades or an extract thereof. Effect of the Invention

[0018] The anxiolytic agent of the present invention contains titan bicus, which contains hyperoside and isoquercitrin in a ratio of (1:3) to (2:1), such as the flowers (including buds) of a specific variety such as titan peach white, or an extract thereof (hereinafter, these are also collectively referred to as titan bicus components), and can improve anxiety-like symptoms. The titan bicus components improve anxiety-like symptoms, and therefore can be used, for example, as an active ingredient or auxiliary ingredient of an anxiolytic agent, and the flowers and buds of titan bicus can be effectively used in a wide range of applications, such as food and beverages, supplements, medicines, and cosmetics.

[0019] Furthermore, the titan bicus component has excellent angiotensin-converting enzyme (hereinafter, referred to as ACE) inhibitory activity, and therefore can be used, for example, as an active ingredient of an ACE inhibitor.

[0020] Titan Peach White flowers (including buds) or their extracts, and Titan Pleiades flowers (including buds) or their extracts have a more specific composition of quercetin glycosides than other varieties. In addition, they have anti-anxiety and ACE inhibitory effects, as well as a variety of other excellent physiological activities, making them useful as supplements, and daily intake can contribute to improving health.

[0021] In particular, the flowers (including buds) of Titan Pleiades or an extract thereof have excellent AGE production inhibitory activity and can therefore be used, for example, as an active ingredient of an AGE production inhibitor.

[0022] Furthermore, the flowers (including buds) of Titan Pleiades or an extract thereof have excellent radical scavenging properties and can therefore be used, for example, as an active ingredient of a radical scavenger. [Brief description of the drawings]

[0023] [Figure 1] FIG. 1 is a schematic diagram of the process for obtaining the titanbicus extract used in the present invention. [Diagram 2] FIG. 1 shows the structural formulas of quercetin and quercetin glycosides. [Diagram 3] FIG. 13 is a graph showing the correlation between the content of quercetin glycoside and DPPH radical scavenging activity. [Figure 4] FIG. 1 is a graph showing the correlation between the content of quercetin glycoside and superoxide scavenging activity. [Diagram 5] FIG. 1 is a graph showing the correlation between the quercetin glycoside content and the AGE production inhibition rate. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0024] In order to find new uses for Titan bicus, the inventors have conducted intensive research, focusing particularly on varieties, and have found that Titan bicus components represented by specific varieties exhibit superior physiological activity. In addition, although Titan bicus (only Titan leia) has been known to be used for food such as edible flowers, it has not been known as a material for ingesting physiologically active ingredients such as supplements, and this time, it has been found that varieties other than Titan leia are useful as materials for supplements due to their effective physiological activity. The present invention is based on such findings.

[0025] The various agents of the present invention, such as anti-anxiety agents, ACE inhibitors, supplements, anti-aging agents, AGE production inhibitors, and radical scavengers, contain specific titanbicus flowers (including buds) or extracts thereof.

[0026] Titanbicus (scientific name Hibiscus x Titanbicus) is a selected hybrid between American hibiscus (Hibiscus moscheutos) and Japanese maple (Hibiscus coccineus).

[0027] The titan bicus used in the present invention contains hyperoside and isoquercitrin, which are quercetin glycosides. In particular, it contains hyperoside and isoquercitrin in a ratio of (1:3) to (2:1), preferably in a ratio of (2:3) to (3:2). As shown in the examples described later, titan bicus contains quercetin glycosides, and many varieties contain a certain amount of isoquercitrin, but the content of hyperoside is low. In contrast, the specific titan bicus used in the present invention contains a specific amount of hyperoside, and exerts excellent physiological activity, including the improvement of anxiety-like symptoms.

[0028] When titan bicus powder is used as the flower (including buds) of titan bicus or an extract thereof, the total amount of hyperoside and isoquercitrin contained in the titan bicus powder is preferably 1.0% by mass or more relative to the total mass of the titan bicus powder. The total amount may be 1.5% by mass or more, 2.0% by mass or more, or 5.0% by mass or less. The content of hyperoside contained in the titan bicus powder is preferably 0.5% by mass or more relative to the total mass of the titan bicus powder, and may be 1.0% by mass or more. The content of isoquercitrin contained in the titan bicus powder is preferably 0.5% by mass or more relative to the total mass of the titan bicus powder, and may be 1.0% by mass or more.

[0029] The content and content ratio of hyperoside, isoquercitrin, and other quercetin glycosides can be calculated by extracting titanbicus flowers (including buds) with 50 vol% methanol and performing HPLC analysis as described in the Examples below.

[0030] The varieties of Titan bicus used in the present invention include Titan Peach White (hereinafter simply referred to as Peach White) and Titan Pleiades (hereinafter simply referred to as Pleiades). Peach White has a white flower with a pale pink color and a red eye in the center. Pleiades has a pink flower with a red eye in the center. For example, Peach White contains hyperoside and isoquercitrin at a ratio of about 1:1, which is preferable from the viewpoint of improving anxiety-like symptoms. In addition, Pleiades contains more isoquercitrin than other varieties, which is preferable from the viewpoint of radical scavenging activity and AGE production inhibitory activity. Furthermore, the quercetin glycoside contained in Peach White powder and Pleiades powder is more easily dissolved in water than each quercetin glycoside alone, and is therefore considered to have a high bioavailability.

[0031] As titan bicus, at least the flowers (including buds) are used. In this case, a mixture of flowers and buds may be used, or only the flowers or only the buds may be used. The raw titan bicus may contain other parts such as leaves, seeds, stems, and roots, but it is preferable that no other parts are included, as this will increase the concentration of ingredients. Also, from the standpoint of hygiene, it is preferable that the calyx is removed from the flowers and buds. In other words, it is preferable that the raw titan bicus does not contain calyx.

[0032] The various agents of the present invention may be processed products such as powders of titan bicus flowers (including buds) as they are, or extracts of such processed products may be used. In other words, "titan bicus flowers (including buds)" also includes titan bicus powder, which is made by powdering them. Figure 1 shows a schematic diagram of the process for obtaining a titan bicus extract as an example of a method for producing the various agents of the present invention.

[0033] In FIG. 1, this manufacturing method includes (a) a processing step and (b) an extraction step. In this method, the flowers and buds of Titanobicus are used as raw materials. These may be in a fresh state or in a frozen state.

[0034] [(a) Processing process] In FIG. 1, the processing step includes a drying step and a pulverization step. The drying method in the drying step is not particularly limited, and natural drying, heat drying, freeze drying, etc. can be performed. In the case of heat drying, the heating temperature can be, for example, 40°C to 80°C. The pulverization method in the subsequent pulverization step is not particularly limited, and can be performed using a well-known pulverizer such as a mill, crusher, or stone mill. It is preferable to use a punching screen in order to make the particle size of the pulverized material somewhat uniform. The particle size and shape of the obtained processed product of the flowers and buds of Titan bicus are not particularly limited, but the moisture content is preferably less than 10% by weight. After the pulverization step, another step (for example, a freeze-drying step or a sterilization step) may be further provided to obtain the processed product.

[0035] [(b) Extraction process] The extraction method is not particularly limited, but examples thereof include solvent extraction and supercritical extraction. Examples of the extraction solvent include water; alcohols such as alcohols having 1 to 4 carbon atoms, such as methanol, ethanol, and isopropanol, and polyhydric alcohols such as 1,3-butylene glycol, ethylene glycol, propylene glycol, and glycerin; ketones such as acetone and methyl ethyl ketone; esters such as ethyl acetate; hydrocarbons such as hexane; ethers such as diethyl ether; and acetonitrile. These extraction solvents may be used alone or in combination of two or more.

[0036] As shown in the examples below, the flowers (including buds) of Titanobicus contain a lot of quercetin glycoside. From the viewpoint of efficiently extracting quercetin glycoside, the extraction solvent is preferably water, alcohols, or a mixture thereof, more preferably aqueous methanol or aqueous ethanol. The alcohol concentration of the aqueous alcohol is, for example, 30 vol% to 90 vol%, and may be 50 vol% to 90 vol%.

[0037] The extraction temperature is, for example, 20° C. to 80° C., and for example, immersion extraction, shaking extraction, ultrasonic extraction, etc. can be used.

[0038] In the present invention, the form of the titan bicus component is not particularly limited, and may be, for example, a powder or liquid (e.g., an extract containing an extraction solvent), etc. Although Fig. 1 shows the steps for obtaining a titan bicus extract, the processed product of the flowers and buds of titan bicus (titan bicus powder) obtained by the (a) processing step may be used in various agents.

[0039] The titanicus used in the present invention preferably contains other quercetin glycosides and quercetin in addition to hyperoside and isoquercitrin. Quercetin glycosides are quercetin represented by the following formula, in which sugars are bound to the hydroxyl groups of quercetin. In quercetin glycosides, the binding positions and number of sugars are not particularly limited.

[0040] [ka]

[0041] The sugars in quercetin glycosides include monosaccharides, oligosaccharides, polysaccharides, etc. The monosaccharides include pentoses such as ribose and arabinose, and hexoses such as glucose, mannose, and galactose, and oligosaccharides and polysaccharides are formed by combining these monosaccharides. When the number of sugars is two or more, the sugars may be the same or different. In addition, the hydroxyl group on the sugar may be substituted with another substituent, for example, modified to an ester bond or ether bond.

[0042] Specific structures of quercetin glycosides are shown in Figure 2. Hyperoside is a compound in which the monosaccharide galactose is bound to the 3-position of quercetin, and isoquercitrin is a compound in which the monosaccharide glucose is bound to the 3-position of quercetin. Other preferred quercetin glycosides include rutin (a disaccharide β-rutinose is bound to the 3-position of quercetin), Q3MG (6-malonylglucose is bound to the 3-position of quercetin), and Q3Samb (a disaccharide sambubiose is bound to the 3-position of quercetin).

[0043] As shown in Figure 2, all quercetin glycosides share the same quercetin structure, but differ in the type and number of sugars bound to them. For example, rutin is bound to a disaccharide, so it is not absorbed in the small intestine, but is absorbed in the large intestine after being metabolized by intestinal bacteria. On the other hand, isoquercitrin, which is bound to one glucose molecule, is absorbed in the small intestine. Hyperoside is also absorbed in the large intestine after being metabolized by intestinal bacteria. All quercetin glycosides are absorbed as free quercetin.

[0044] Titan bicus ingredients contain multiple quercetin glycosides (e.g., hyperoside, isoquercitrin, and rutin), which are thought to be effective in maintaining activity in the body, even if they have a common quercetin structure, because there are differences in the place and time it takes for them to be absorbed into the body. In addition, it has been reported that isoquercitrin is more easily absorbed into the body than quercetin or rutin, so if the titan bicus ingredients contain more isoquercitrin, it is thought to be advantageous in terms of absorbability into the body.

[0045] The titan bicus component may contain quercetin glycosides other than those mentioned above, such as other quercetin rutinosides, other quercetin glucosides, other quercetin galactosides, quercetin rhamnosides, and quercetin glucuronides.

[0046] When titan bicus powder is used as the titan bicus component, the total amount of quercetin and quercetin glycoside contained in the titan bicus powder is preferably 2.0% by mass or more, and may be 2.5% by mass or more, based on the total mass of the titan bicus powder.

[0047] In addition, the titan bicus component may contain components (other components) other than quercetin and quercetin glycoside. The other components preferably contain flavonoids (especially kaempferol) and proanthocyanidin. In addition, myricetin, luteolin, and apigenin may not be included as flavonoids.

[0048] After ingestion, polyphenols such as quercetin glycoside are immediately excreted from the body through a process called conjugation. In particular, when a large amount of a specific component is ingested, almost all of it is excreted. However, vegetables and fruits contain multiple similar components, and it is said that when these are ingested together, some of the components are absorbed into the body without being conjugated. In addition, in daily meals in which multiple different types of flavonoids are ingested at the same time, it is thought that the metabolic pattern changes depending on the combination of ingredients. Although the effects of quercetin glycoside and other flavonoids in the body have not been fully elucidated, the simultaneous ingestion of multiple different types of flavonoids is expected to have a different effect than when a large amount of a single component is ingested. Therefore, it is speculated that the inclusion of other flavonoids (such as kaempferol) as other ingredients will be advantageous for absorption in the body.

[0049] The anti-anxiety agent of the present invention contains the above-mentioned flower (including buds) of Titanobicus or an extract thereof, and shows an effect of improving anxiety-like symptoms. Here, the anxiety-like symptoms refer to states such as anxiety, impatience, and fear, as well as symptoms including lethargy, apathy, inhibitory symptoms included in depression-like symptoms, and sleep disorders caused by anxiety.

[0050] In the present invention, the degree of the improving effect on anxiety-like symptoms can be evaluated using an anxiety level evaluation system such as an elevated plus maze test, etc. Specifically, in the elevated plus maze test, when the anxiety level after administration of the test substance is lower than the anxiety level before administration, it can be determined that the anxiolytic effect is exerted.

[0051] Quercetin glycosides are widely found in various vegetables and fruits, and hyperoside and isoquercitrin are known to have antidepressant effects (Reference 1; Biol. Pharm. Bull. 24(7), 848-851). In addition, hyperoside and isoquercitrin derived from rafuma have been approved as functional food-related ingredients, as they are useful for improving sleep quality. In addition to the results of the elevated plus maze test described below, these reports also show that the Titanbicus ingredients mentioned above have an effect of improving anxiety-like symptoms.

[0052] The ACE inhibitor of the present invention contains the above-mentioned flower (including buds) of Titanobicus or an extract thereof, and exhibits ACE inhibitory activity. Hypertension, which is one of the lifestyle-related diseases, causes heart diseases such as myocardial infarction and cerebrovascular disorders such as stroke. There are four main blood pressure regulating systems in the body, among which the renin-angiotensin system is said to play a particularly important role. On the other hand, it is known that the intake of plant-derived foods suppresses blood pressure rise. The reasons for this are thought to be that plant-derived foods such as vegetables contain many ingredients that suppress blood pressure rise, such as dietary fiber, potassium, and γ-aminobutyric acid, and that blood pressure rise is suppressed by inhibiting ACE, one of the enzymes involved in hypertension. It is known that blood pressure rises in the body when angiotensin I is converted to angiotensin II, which has a strong vasoconstricting effect, by ACE. Therefore, inhibiting ACE activity is particularly effective in suppressing excessive blood pressure rise. For this reason, in recent years, many functional foods aimed at preventing hypertension have been sold, and food ingredients with ACE inhibitory activity have attracted attention. There have been no reports to date that titan bicus has ACE inhibitory effects, and this study has discovered that titan bicus can be useful as a food ingredient for preventing high blood pressure.

[0053] The anti-aging agent of the present invention contains the above-mentioned titanbicus flower (including buds) or an extract thereof, and exhibits an anti-aging effect. This anti-aging effect is exerted based on at least one of the effects of inhibiting AGE production and radical scavenging. Note that the anti-aging effect of the titanbicus component is not limited to the anti-aging effect exerted based on the above-mentioned effects.

[0054] The AGE production inhibitor of the present invention contains the above-mentioned Titanobicus flower (including buds) or an extract thereof, and exhibits an AGE production inhibitory effect. Anti-glycation has been attracting attention in recent years in beauty research. Glycation is the non-enzymatic binding of reducing sugars such as glucose to proteins, and the substances produced by this are called advanced glycation endproducts. It has been suggested that advanced glycation endproducts (AGEs) are involved in diabetic complications, arteriosclerosis, neurodegenerative diseases, and the like, and it has also become clear that they are closely related to skin aging.

[0055] AGEs increase with age, but when the Maillard reaction occurs in collagen, a protein in the skin, the amino groups of lysine residues in the protein react nonenzymatically with the carbonyl groups of sugars, producing AGEs and cross-linking the collagen parts. When a cross-linked structure is formed, the skin's original elasticity is lost. It is also said to lead to the development of wrinkles and sagging. Thus, preventing glycation is important not only for health but also for beauty, and inhibiting the production of AGEs can be said to lead to anti-aging. As shown in the examples below, the Pleiades flower (including buds) or its extracts are particularly effective in inhibiting the production of AGEs.

[0056] The radical scavenger of the present invention contains the above-mentioned Titanobicus flower (including buds) or an extract thereof, and exhibits a radical scavenging effect. In anti-aging, radicals such as active oxygen and free radicals that oxidize biological components have also attracted attention. Radicals refer to atoms or molecules having one or more unpaired electron pairs, and include hydroxyl radicals, superoxide, DPPH radicals, and the like. Skin is particularly susceptible to direct stimulation by environmental factors such as ultraviolet rays, and radicals are easily generated, and the generated radicals are thought to decompose, denature, crosslink, and the like in biological tissues such as collagen, resulting in aging such as wrinkle formation and loss of elasticity of the skin. Therefore, it is believed that by scavenging (capturing) radicals, it is possible to prevent and improve skin aging such as wrinkle formation and loss of elasticity, and diseases induced by oxidative stress of radicals (diabetic complications, arteriosclerosis, neurodegenerative diseases, etc.). As shown in the examples described below, the Pleiades flower (including buds) or an extract thereof is particularly excellent in radical scavenging effect.

[0057] The various agents of the present invention can be used as foods and beverages, supplements, medicines, cosmetics, etc., or as ingredients to be blended into these. For example, they can be used as health functional foods (specified health foods, functional foods, nutritional functional foods) that claim or display the prevention or improvement of anxiety-like symptoms, high blood pressure, anti-aging, etc., or as additives thereto.

[0058] The various agents of the present invention are not particularly limited in terms of dosage form or application method, and can be, for example, oral, topical skin agents, etc. In the case of oral administration, the dosage form can be, for example, tablets, liquids, powders, capsules, etc. In the case of topical skin agents, the dosage form can be, for example, liquids, lotions, emulsions, ointments, creams, etc.

[0059] The supplement of the present invention is taken orally and contains the above-mentioned titan bicus flower (including buds) or an extract thereof. The titan bicus component has excellent physiological activity and is therefore useful as a supplement. For example, the supplement is taken for the purpose of nutritional supplementation or health promotion, and is in the form of a tablet, liquid, powder, capsule, or the like, and contains the titan bicus component (preferably peach white powder or Pleiades powder) in an amount of, for example, 0.1% to 50% by mass relative to the total amount of the supplement. The content of the titan bicus component may be 0.5% to 30% by mass, or 1% to 20% by mass.

[0060] For example, when taking supplements, it is preferable to take the amount of hyperoside contained in the titan bicus ingredients at 0.1 mg to 10 mg (preferably 0.3 mg to 3 mg) per day, and the amount of isoquercitrin contained in the titan bicus ingredients at 0.1 mg to 10 mg (preferably 0.3 mg to 3 mg) per day. EXAMPLES

[0061] [1. Quercetin glycoside content in eight cultivars of Titanobicus] (a) Extraction of quercetin glycoside Quercetin glycosides were extracted from the buds of eight varieties of Titanobicus (Cassiopeia, Sylphid, Uranus, Peach White, Neon, Artemis, Leia, and Pleiades). The extraction solvent used was a mixture of acetonitrile, 2-propanol, and ethanol in a 2:1:1 ratio. Extraction was performed using the following procedure. 1: Place one bud into a 100ml glass beaker. 2: 50 ml of extraction solvent was added per 8.7 g of fresh weight of buds. 3: Crush the buds slightly with a spoon and completely submerge them in the extraction solvent. 4: Allow to stand at room temperature in a dark place for 20 hours. 5: After 20 hours, the extract was filtered through a 0.45 μm syringe filter. 6: The filtered extract was diluted 2-fold and 4-fold with methanol. 7: The quercetin glycosides contained in the three samples, the extract filtered in 5 above and the diluted solution in 6 above, were quantitatively analyzed.

[0062] (b) Analysis of extract components Detector: Photodiode array detector (measurement wavelength 254 nm) High performance liquid chromatography (HPLC): Agilent 1100 Series (Agilent) Column: ZORBAX Eclipse XDB-C18 (Agilent; column inner diameter 4.6 mm, column length 250 mm, particle size 5 μm) Column temperature: 40℃ Mobile phase A: Acetonitrile (100%) Mobile phase B: 0.01% formic acid Flow rate: 1.000ml / min Delivery of each mobile phase: The mixing ratio of mobile phase A and mobile phase B was changed as shown in Table 1.

[0063] [Table 1]

[0064] Quantitative analysis was performed for rutin, hyperoside, isoquercitrin, and quercetin using calibration curves obtained from the respective standard substances (rutin trihydrate, hyperoside, isoquercitrin, and quercetin dihydrate). Each calibration curve was prepared by the above HPLC analysis using a methanol solution (standard stock solution), a 10-fold diluted solution, and a 100-fold diluted solution of each standard substance. As it was not possible to obtain standard substances for Q3Samb and Q3MG, the calibration curve of rutin was used for the quantification of Q3Samb, and the calibration curve of isoquercitrin was used for the quantification of Q3MG. The method of quantitative analysis using substances with similar structures as model compounds is widely used.

[0065] The detection times for quercetin and each quercetin glycoside under the above HPLC analysis conditions are as follows: Q3Samb: Approx. 4.1 min Rutin: approx. 4.8 min Hyperoside: approx. 5.8 min Isoquercitrin: approx. 6.0 min Q3MG: Approx. 6.6 min Quercetin: approx. 16.2 min

[0066] The quercetin glycoside content (mg / 100g) per 100g fresh weight of buds in eight cultivars of Titanobicus is shown in Table 2.

[0067] [Table 2]

[0068] As shown in Table 2, in the analysis results per 100g of fresh bud weight, Leia, which has a history of being eaten as an edible flower, had the second highest total amount of quercetin glycoside. Among the eight varieties, the variety with the highest concentration of quercetin glycoside in the buds was Pleiades. Hyperoside also showed a particularly large variation between varieties, with none detected in Cassiopeia, Sylfide, Uranus, or Neon, but the highest amount detected in Peach White, followed by Pleiades.

[0069] In the cultivars in which hyperoside was detected, the ratio of hyperoside to isoquercitrin was about 1:1 in Peach White and about 1:2 in Pleiades. In contrast, the ratios were about 1:8 in Leia and about 1:8 in Artemis. Peach White and Pleiades had a higher ratio of hyperoside to isoquercitrin than the other cultivars.

[0070] [2. Biological activity test of three varieties of Titan Vikas powder] (a) Manufacturing of Titan Vikas Powder The raw materials used were buds of three varieties (Leia, Peach White, and Pleiades). The raw material Titan bicus buds were harvested with the calyx still attached by cutting off the base of the bud. In addition, blooming Titan bicus flowers were also harvested with the calyx still attached. The harvested buds and flowers were promptly decanted and frozen for storage. The frozen buds and flowers were then powdered and sterilized using the processing procedure shown in Table 3 below. Note that Table 3 shows the results for Peach White powder, but the other Titan bicus powders (Leia powder, Pleiades powder) were also manufactured using the same procedure.

[0071] [Table 3]

[0072] As a result of powdering and sterilization, 1385g of peach white powder was produced from 16.68kg of peach white buds (frozen product). The moisture content of the obtained peach white powder is very low at the 5% level, and the general viable bacteria count is also sufficiently low for food, so it can be stored at room temperature (preferably in a dark place).

[0073] (b) Component analysis 0.2g of peach white powder was weighed into a 15ml centrifuge tube, and 1ml of 50vol% methanol (methanol diluted 2-fold with ultrapure water) was added as an extraction solvent, and the components contained in titan bicus powder were eluted in 50vol% methanol. The extract was filtered through a 0.45μm filter (filter material: hydrophilic mixed cellulose ester, diameter 25mm, pore size: 0.45μm, Shimadzu GLC Co., Ltd.) to obtain an extract. This extract was used as a sample and component analysis was performed under the above HPLC conditions. Table 4 shows the results for peach white powder.

[0074] [Table 4]

[0075] As shown in Table 4, the content of hyperoside and isoquercitrin was detected in the same ratio of about 1:1 in Peach White powder as in the fresh buds. From Table 4, it was found that Peach White powder contains more than 3000mg of quercetin glycoside in total per 100g, of which more than 800mg of hyperoside (specifically more than 1000mg) and more than 800mg of isoquercitrin (specifically more than 1000mg). The results of Leia powder and Pleiades powder were roughly the same as those of the fresh buds.

[0076] A separate quantitative analysis of kaempferol revealed that the kaempferol content in 100g of Peach White Powder was 0.62mg / 100g, the kaempferol content in 100g of Leia Powder was 0.22mg / 100g, and the kaempferol content in 100g of Pleiades Powder was 2.59mg / 100g.

[0077] (c) DPPH radical scavenging activity The evaluation was based on Reference 2 (Food Function Research Methods, Korin, 2000, p.218-220). 50 vol% ethanol was used as the extraction solvent. The DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity of Leia powder, Peach White powder, and Pleiades powder was measured by spectrophotometry (visible). The results are shown in Table 5.

[0078] [Table 5]

[0079] As shown in Table 5, it was found that Titan Vicus powder has DPPH radical scavenging activity. The DPPH radical scavenging activity was stronger in the order of Leia powder, Peach White powder, and Pleiades powder. Note that TE refers to Trolox equivalent, and the activity indicated by 1 μmol of Trolox was used as a unit.

[0080] Based on the above results, the correlation between the content of quercetin glycosides and DPPH radical scavenging activity was analyzed. The quantitative values ​​of rutin, hyperoside, isoquercitrin, and quercetin were analyzed. The quantitative values ​​used in this analysis were the results of analysis by the Japan Food Research Laboratories (see Table 6).

[0081] [Table 6]

[0082] Analysis of the correlation between the isoquercitrin content and DPPH radical scavenging activity in the three varieties of titan bicus powder revealed an extremely high correlation (see Figure 3). On the other hand, no clear correlation was found between quercetin glycosides other than isoquercitrin and DPPH radical scavenging activity. In addition, even if the y-intercept of the approximation equation is 0, R 2 = 0.996, suggesting that the DPPH radical scavenging activity of titan bicus powder may depend on the concentration of isoquercitrin.

[0083] (d) Superoxide scavenging activity Next, superoxide radicals, which are also present in the body, were used as radical species. The superoxide scavenging activity of Leia powder, Peach White powder, and Pleiades powder was measured by electron spin resonance (ESR) method. The results are shown in Table 7. The units in the table are expressed as scavenging ability equivalent to the units defined by JM MacCord and I. Fridovich [J. Biol. Chem., 224, 6049 (1969)].

[0084] [Table 7]

[0085] As shown in Table 7, Pleiades powder had the highest superoxide scavenging activity, followed by Peach White powder and Leia powder. This order was the same as the order of the quercetin glycoside content, suggesting a correlation with quercetin glycoside in the superoxide scavenging activity as well. Therefore, similar to (c) above, the correlation between the content of various quercetin glycosides and superoxide scavenging activity was analyzed.

[0086] Analysis of the correlation between the isoquercitrin content and superoxide scavenging activity in three types of titan bicus powder revealed a high correlation (see Figure 4(a)). Analysis of the correlation between the total amount of rutin, isoquercitrin, and quercetin and superoxide scavenging activity also revealed a high correlation (see Figure 4(b)). It was suggested that isoquercitrin is responsible for part of the superoxide scavenging activity in titan bicus powder, and that rutin and quercetin also have a certain degree of effect.

[0087] (e) AGE production inhibitory activity The effect of titan bicus powder on AGE production was investigated by measuring the fluorescence intensity derived from AGEs produced by the glycation reaction of D-glucose and bovine serum albumin (BSA) in the presence of titan bicus powder, using this as an indicator.

[0088] 50 vol% ethanol was added to the titan bicus powder, and the mixture was shaken for 10 minutes using a shaker, followed by ultrasonic treatment for 10 minutes.The mixture was then centrifuged (3000 r / min, 10 minutes) and the supernatant was separated.The resulting supernatant was used as a 50 mg / mL test solution.

[0089] 100μL of BSA solution, 500μL of D-glucose solution, 20μL of test solution, and 380μL of phosphate buffer were added to a 1.5mL microtube, and the mixture was heated at 60℃ for 48 hours to prepare a reaction solution. The same test was performed using a sample containing phosphate buffer instead of the test solution as an untreated control, and a sample containing phosphate buffer instead of the D-glucose solution as a blank. 20μL of the reaction solution was dispensed into a 96-well plate, and 200μL of water was added and mixed.

[0090] The fluorescence intensity derived from AGEs was measured (excitation wavelength 370 nm, fluorescence wavelength 440 nm) using a microplate reader (SpectraMax M2e, Molecular Devices, LLC.).

[0091] The inhibition rate of AGE production was calculated from the fluorescence intensity of each reaction solution relative to the fluorescence intensity of the untreated control according to the following formulas (1) and (2). AGEs production rate (%) = [(fluorescence intensity of reaction solution) / (fluorescence intensity of untreated control)] × 100 (1) AGEs production inhibition rate (%) = 100-AGEs production rate...(2) It should be noted that each fluorescence intensity in the above formula (1) is a value obtained by subtracting the blank fluorescence intensity.

[0092] [Table 8]

[0093] As shown in Table 8, it was found that at a final concentration of 1 mg / mL, each Titan Bicus powder was able to inhibit AGE production by 27% for Leia powder, 30% for Peach White powder, and 36% for Pleiades powder. There are few food ingredients (spices, etc.) that inhibit AGE production by more than 30%, and the results for these three varieties of Titan Bicus powder indicate that Titan Bicus powder has a certain anti-glycation effect, suggesting that quercetin glycoside is involved in this result.

[0094] As mentioned above, it was suggested that titan bicus powder has AGE production inhibitory activity, and it was predicted that the higher the quercetin glycoside content, the higher the AGE production inhibition rate. Therefore, the correlation between the quercetin glycoside content and the AGE production inhibition rate was analyzed. A correlation was analyzed between isoquercitrin and the AGE production inhibition rate in the three varieties of titan bicus powder, and a correlation was found (see Figure 5 (a)). In addition, a correlation was also analyzed between the total content of isoquercitrin and rutin and the AGE production inhibition rate, and a high correlation was found (see Figure 5 (b)). These results suggest that isoquercitrin is responsible for part of the AGE production inhibition in titan bicus powder, and that rutin also has a certain effect.

[0095] [3. Solubility of Peach White Powder in Water] The solubility of peach white powder in water was evaluated. As a comparison control, the same test was also performed on the standard substances of rutin, isoquercitrin, and hyperoside used in the quantitative analysis of various quercetin glycosides.

[0096] Peach white powder and various quercetin glycoside standards were mixed with ion-exchanged water or 50% methanol solution. The above solution was filtered through a 0.45 μm filter, and quantitative analysis of various quercetin glycosides in the filtrate was carried out using HPLC. In this case, quercetin glycosides that did not dissolve in the solvent were removed by the 0.45 μm filter. The solubility in water was evaluated by calculating the "area in ion-exchanged water" / "area in 50% methanol solution." The results are shown in Table 9.

[0097] [Table 9]

[0098] The test results showed that the solubility of the standard substances rutin, isoquercitrin, and hyperoside in ion-exchanged water was low. On the other hand, the various quercetin glycosides contained in Peach White Powder were found to be more soluble in water than those in the standard substances. In addition to these quercetin glycosides, Peach White Powder contains various other components, which are presumed to increase the solubility of quercetin glycosides in water. As highly refined quercetin glycosides have low solubility in water, Peach White Powder is considered to be more advantageous in terms of bioavailability.

[0099] [4. Anti-anxiety test using Peach White powder] (a) Administration of the test substance The anti-anxiety effect of Peach White powder was verified by the "elevated plus maze test using mice," which is a widely recognized method for verifying anti-stress and anti-anxiety effects. A group was also administered the antidepressant drug "diazepam" as a positive control.

[0100] Four-week-old Crl:CD1 (ICR) male mice were acclimated for at least 6 days. Based on the body weight of the mice on the day of grouping, they were assigned to four groups of 10 mice each using the weight stratification method using EXSUS (Version 10.1.3, EPS Corporation) so that the average body weight of each group was equal.

[0101] The test groups were as follows: Group 1: Control group Group 2: Low-dose Peach White powder group (total concentration of isoquercitrin and hyperoside: 2.5 mg / kg) Group 3: High dose of peach white powder (total concentration of isoquercitrin and hyperoside: 10 mg / kg) Group 4: Diazepam group (3 mg / kg)

[0102] The test substances used were peach white powder and diazepam (positive control). When these test substances were orally administered to mice, 0.5 w / v% methylcellulose 400 solution (Fujifilm Wako Pure Chemical Industries, Ltd.) was used as the vehicle. In the case of peach white powder, the total concentration of isoquercitrin and hyperoside was adjusted to 0.25 mg / mL or 1 mg / mL (administration dose: 2.5 mg / kg, 10 mg / kg). In the case of diazepam, it was adjusted to 0.3 mg / mL (administration dose: 3 mg / kg).

[0103] For group 1, the vehicle was orally administered to the mice three times in total, 24 hours, 5 hours, and 1 hour before the elevated plus maze test. For groups 2 and 3, peach white powder solution was orally administered three times in total, 24 hours, 5 hours, and 1 hour before the elevated plus maze test. For group 4, the vehicle was orally administered 24 hours and 5 hours before the elevated plus maze test, respectively, and a diazepam solution (3 mg / kg) was orally administered 1 hour before the test.

[0104] (b) Elevated plus maze test After acclimating the mice under 200 Lux for more than 5 hours (administration of the test substance 5 hours and 1 hour before was performed under 200 Lux), the mice were placed headfirst into a closed arm of an elevated plus maze apparatus and measured for 5 minutes. Five items were measured: number of closed arm entries, number of open arm entries, time spent in closed arm (sec), time spent in open arm (sec), and locomotor activity (number of open arm entries + number of closed arm entries). An entry into an arm was judged when all four limbs entered the arm. When a mouse left a closed arm and went into the center area and then returned to the same closed arm, the number of entries was counted as 1.

[0105] (c) Data analysis The results obtained in the test were expressed as mean ± standard error (Mean ± SE). Furthermore, for the number of closed arm entries and the number of open arm entries, in the multi-group comparison between the control group and the peach white powder group, the difference in the mean values ​​was examined using the Bartlett test if the variances were equal, and the Dunnett test if the variances were unequal, and the Steel test if the variances were unequal. In the comparison between the two groups between the control group and the diazepam group, the F test was performed, and the difference in the mean values ​​was examined using the Student t test if the variances were equal, and the Aspin Welch t test if the variances were unequal. Furthermore, for the closed arm entry rate and the open arm entry rate, the Steel test was used in the multi-group comparison between the control group and the peach white powder group, and the Wilcoxon test was used in the comparison between the control group and the diazepam group. All tests were performed using two-sided tests with a significance level of 5%, and EXSUS (Version 10.1.3, EPS Corporation) linked to SAS (Version 9.4, SAS Institute Inc.) was used for each statistical analysis. The results are shown in Table 10.

[0106] [Table 10]

[0107] As shown in Table 10, the average open arm entry rate in the control group was 22.6%, while the average open arm entry rate in the high dose group of Peach White Powder was 26.5%, which was higher than that of the high dose group of Peach White Powder. This result suggests that the administration of Peach White Powder (10 mg / kg) tends to suppress the anxiety of the mice. In this test, the test substance was administered three times, 24 hours, 5 hours, and 1 hour before the test, but it is expected that a more significant difference will be obtained by administering, for example, 10 mg / kg for several consecutive days.

[0108] In addition, in an acute toxicity test in which a single dose of 2000mg / kg of Peach White Powder was administered to a different strain of mice from this test, no abnormalities such as deaths were observed. Therefore, it can be said that Peach White Powder is a very safe food ingredient. It is very significant that a highly safe natural product has a tendency to suppress anxiety.

[0109] [5. Angiotensin-converting enzyme inhibition test with Titan Bicus powder] In this test, the dipeptide broken down by ACE from the substrate (Hip-His-Leu) was fluorescentized with ortho-phthalaldehyde (OPA), and the fluorescence intensity of the reaction product was measured to determine ACE activity, and the effect of Peach White powder or Pleiades powder on ACE activity was evaluated.

[0110] 50 vol% ethanol was added to Peach White powder or Pleiades powder, and the mixture was shaken for 10 minutes on a shaker, followed by ultrasonic treatment for 10 minutes. After ultrasonic treatment, the mixture was centrifuged (3000 r / min, 10 minutes) and the supernatant was separated. The resulting supernatant was used as a 50 mg / ml test solution stock solution. The test solution stock solution was diluted with HEPES buffer to prepare test solutions with the sample concentrations shown in Table 11.

[0111] 25 μl of the test solution prepared above was added to a 96-well microplate, followed by 25 μl of ACE solution, and the plate was left to stand at 37°C for 5 minutes. The plate was tested in the same manner as above, with HEPES buffer added instead of the test solution as an untreated control, and with phosphate buffer added instead of the ACE solution as a blank. After leaving the plate to stand, 25 μl of substrate solution was added to stop the reaction, and 25 μl of OPA solution was added and the plate was left to stand at room temperature for 20 minutes. Furthermore, 25 μl of 0.1 mol / l hydrochloric acid was added and the plate was left to stand at room temperature for 10 minutes.

[0112] The main reagents are as follows: HEPES buffer: Sodium chloride and HEPES [Sigma-Aldrich] were mixed with water to adjust the pH to 8.3, and then Triton-X was added and the resulting solution was adjusted to 0.1 mol / l with water (containing 0.3 mol / l sodium chloride and 0.01% Triton-X). ACE solution: ACE (derived from rabbit lung) [Sigma-Aldrich] at 13 mU / ml in phosphate buffer. Substrate solution: Hip-His-Leu [Peptide Institute, Inc.] at 8 mmol / l in HEPES buffer. OPA solution: A 1% solution of OPA [Fujifilm Wako Pure Chemical Corporation] in methanol.

[0113] The fluorescence intensity of the reaction product was measured using a microplate reader [SpectraMax M2e Molecular Devices, LLC.] (excitation wavelength: 355 nm, emission wavelength: 460 nm).

[0114] The ACE activity and inhibition rate were calculated from the fluorescence intensity of each test solution relative to that of the untreated control using the following formula, where each fluorescence intensity was a value obtained by subtracting the fluorescence intensity of the blank. ACE activity (%) = (fluorescence intensity of sample solution) / (fluorescence intensity of untreated control) x 100 ACE activity inhibition rate (%) = 100 - ACE activity (%)

[0115] The final concentration of the sample was calculated according to the following formula. Final concentration (mg / ml) = Sample concentration (mg / ml) x Sa / (Sa+Ace+Sub+Naoh+Opa+Hcl) = sample concentration (mg / ml) ÷ 6 Sa: Test solution volume (25 μl) Ace: Amount of ACE solution dispensed (25 μl) Sub: Substrate solution volume (25 μl) Naoh: 0.1 mol / l sodium hydroxide solution dispensed (25 μl) Opa: OPA solution dispensed volume (25 μl) Hcl: 0.1 mol / l hydrochloric acid dispensed (25 μl)

[0116] In addition, for Peach White Powder or Pleiades Powder, a logarithmic approximation curve was created from a graph with the ACE activity inhibition rate on the vertical axis and the sample concentration on the horizontal axis, and the IC 50The 50% inhibitory concentration was calculated. The results are shown in Table 11.

[0117] [Table 11]

[0118] As a result of the test, IC 50 The IC value was 0.163 (50% inhibition at a final concentration of 0.163 mg / ml), and the IC 50 The value was 0.134 (50% inhibition rate at a final concentration of 0.134mg / ml), which is a high figure. Reference 3 (Journal of the Japanese Society for Food Science and Technology, Vol. 59, No. 7, pp. 348-353 (2012)) reports the ACE activity inhibition rate of 80 types of vegetable powders, but even compared to powders of natural products such as vegetables, the ACE inhibitory activity of this Peach White Powder and Pleiades Powder is extremely high. For example, if blood pressure can be controlled through daily diet, it would be very desirable from the perspective of preventing lifestyle-related diseases.

[0119] The above results also show that Pleiades powder has a higher ACE inhibitory activity than Peach White powder. As shown in Table 6 above, the content of quercetin glycosides (four components) in Peach White powder and Pleiades powder is almost the same, at 2.800g / 100g and 2.820g / 100g. However, the ACE inhibitory activity (IC 50 Since the ACE inhibitory activity (A value) is higher in Pleiades powder, it was speculated that there is a correlation between the content of specific quercetin glycosides, rather than the total amount of quercetin glycosides, and the ACE inhibitory activity. The ACE inhibitory activity of Pleiades powder is about 1.2 times that of Peach White powder, and the isoquercitrin content of Pleiades powder is about 1.3 times that of Peach White powder. From this, it is speculated that the ACE inhibitory activity of titanbicus flowers (including buds) or their extracts depends on the isoquercitrin content.

[0120] [6. Proanthocyanidin content in Titan Bicus powder] An adult consumes about 500 liters of oxygen per day, and it is thought that 3-10% of this is converted into active oxygen in the body. Active oxygen and free radicals generated in the human body are the cause of aging and various lifestyle-related diseases. On the other hand, it has been reported that some synthetic antioxidants are suspected of being carcinogenic, so effective and safe natural antioxidants are promising. Proanthocyanidins, a type of polyphenol, are found in small amounts in various foods, such as fruits such as grapes, strawberries, and apples, wheat and other grains such as barley, beans such as black beans and red beans, and processed foods made from these. Proanthocyanidins exhibit strong antioxidant properties, especially in polar solvents such as water. For example, procyanidin dimers, a representative proanthocyanidin, have a strong scavenging effect on hydrophilic peroxy radicals, and it has been found that one molecule of the dimer can capture eight hydrophilic radicals. This number of scavenging is the largest among known antioxidants. Proanthocyanidins are known to have strong antioxidant properties, but there have been no reports on whether proanthocyanidins are contained in the flowers (including buds) of Titanobicus.

[0121] The proanthocyanidins contained in Titan Bicus powder (Leia powder, Peach White powder, Pleiades powder) were quantitatively analyzed using the vanillin-sulfuric acid method. The method is as follows. The total proanthocyanidin content was calculated as the (+)-catechin equivalent ((+)-catechin standard, Fujifilm Wako Pure Chemical Corporation). 1: 2, 3, 4, 6, 8, and 10 mg of Titan Bicus powder were weighed out and placed in a 15 ml centrifuge tube, and 1 ml of methanol was added. Note that the (+)-catechin solution was prepared in methanol and diluted to 0.01 mg / ml, 0.02 mg / ml, 0.05 mg / ml, 0.10 mg / ml, 0.15 mg / ml, 0.20 mg / ml, and 0.25 mg / ml with methanol to prepare the analysis samples. 2 ml of 2:1% (w / v) vanillin / methanol was added. 3: 2 ml of 25% (v / v) sulfuric acid / methanol was added. 4. Shake at 30°C for 15 minutes. 5: 1 ml of methanol was added. 6: The supernatant was obtained by centrifugation (600 g x 2 minutes, room temperature). 7: Quantification was performed by measuring the absorbance of the supernatant at 500 nm. The results are shown in Table 12.

[0122] [Table 12]

[0123] As shown in Table 12, it was found that Titan Bicus powder contains proanthocyanidins. The content was the highest among the three varieties in Pleiades powder, followed by Leia powder and Peach White powder.

[0124] Here, it has been reported that there is a very high correlation between the total amount of proanthocyanidins in red rice, which is said to contain proanthocyanidins, and DPPH radical scavenging activity (r=0.948) (Reference 4; Journal of the Japanese Society for Food Science and Technology, Vol. 58, No. 12, pp. 576-582 (2011)). On the other hand, the DPPH radical scavenging activity of titan bicus powder is highly correlated with the concentration of isoquercitrin (see Table 5 and Figure 3). In addition, there is a possibility that components other than quercetin glycosides are involved in the superoxide scavenging ability of titan bicus powder. Based on this, although both titan bicus powder and red rice contain proanthocyanidins, it is possible that their radical scavenging mechanisms are different.

[0125] There are also reports that proanthocyanidins suppress cognitive decline through the intestinal flora (Reference 5; Chemistry and Biology, Vol. 60, No. 3, pp. 137-143 (2022)). As mentioned above, the elevated plus maze test showed that titan bicus powder improved anxiety-like symptoms in mice, and it is possible that not only the quercetin glycoside contained in titan bicus powder but also proanthocyanidins act on the mouse brain. [Industrial Applicability]

[0126] The anxiolytic agent, angiotensin converting enzyme inhibitor, anti-aging agent, AGEs production inhibitor, radical scavenger, and supplement of the present invention exhibit various physiological actions by containing, for example, Titan bicus containing hyperoside and isoquercitrin in a ratio of (1:3) to (2:1), specifically Titan peach white and Titan pleiades flowers (including buds) or their extracts, and can be effectively used for a wide range of applications such as food and beverages, supplements, medicines, and cosmetics. Specifically, the various agents can be used as food ingredients and beauty ingredients that appeal to anti-anxiety, blood pressure suppression, and antioxidant properties. In addition, the various agents can be said to be upcycled products that add new added value to the flowers and buds of Titan bicus that were unavoidably discarded because they could not be shipped for horticultural or ornamental purposes, and can also contribute to the achievement of the SDGs.

Claims

1. An anti-anxiety agent comprising a flower (including buds) of Titanobicus or an extract thereof, the flower (including buds) containing hyperoside and isoquercitrin in a ratio of (1:3) to (2:1).

2. The anti-anxiety agent described in claim 1, characterized in that the titan bicus flower (including buds) or its extract contains titan bicus powder, and the total amount of the hyperoside and the isoquercitrin contained in the titan bicus powder is 1.0 mass% or more relative to the total mass of the titan bicus powder.

3. An anti-anxiety agent comprising the flowers (including buds) of Titan Peach White (Ministry of Agriculture, Forestry and Fisheries Variety Registration No. 21813) or an extract thereof.

4. An angiotensin-converting enzyme inhibitor comprising a flower (including buds) of Titanobicus or an extract thereof, the flower (including buds) containing hyperoside and isoquercitrin in a ratio of (1:3) to (2:1).

5. An angiotensin-converting enzyme inhibitor comprising the flowers (including buds) of Titan Peach White (Ministry of Agriculture, Forestry and Fisheries Variety Registration No. 21813) or an extract thereof.

6. A supplement characterized by containing powder of Titan Peach White (Ministry of Agriculture, Forestry and Fisheries Variety Registration No. 21813) flowers (including buds) or powder of Titan Pleiades flowers (including buds).

7. An AGEs production inhibitor characterized by containing Titan Pleiades flowers (including buds) or an extract thereof.

8. A radical scavenger comprising Titan Pleiades flowers (including buds) or an extract thereof.

Images