Hair composition and scalp composition

Abstract

To provide a composition exhibiting superior efficacy, stability in quality, and biological safety, which energizes dermal papilla cells regulating the hair cycle, contributes to normalization of the hair cycle, suppresses scalp inflammation, and enhances both moisture and cohesion of hair.SOLUTION: The present invention provides a composition comprising, as an active ingredient, an extract of sage (Salvia officinalis) belonging to the genus Salvia of the family Lamiaceae.SELECTED DRAWING: Figure 1

Description

[Technical Field]

[0001] The present invention relates to a composition for hair or a composition for scalp, which is highly safe for the human body and contains an extract of a plant of the Labiatae family as an active ingredient. [Background technology]

[0002] In recent years, due to various factors such as aging, stress or ultraviolet rays, hair gloss and stiffness are reduced, scalp inflammation occurs, and not only men but also women are increasingly suffering from scalp and hair troubles, so that various scalp cosmetics and hair cosmetics have been proposed in response to this.In addition, research has been carried out on male pattern baldness (androgenetic alopecia) and female pattern baldness (male pattern baldness that occurs in women), and it has been revealed that male hormones are involved in these alopecias, so that various anti-androgen drugs have been proposed as agents for improving hair troubles.

[0003] In order to prevent the above-mentioned aging and deterioration of hair or scalp and to maintain a youthful state, and further based on research into the mechanisms of hair and scalp damage and hair loss, various hair agents, hair growth and tonics, and hair loss inhibitors (hereinafter referred to as hair growth agents, etc.) have been proposed. For example, amino acids, hyaluronic acid or its salts, collagen, etc. are known as moisturizing agents for hair and scalp, and minoxidil and adenosine are known as active ingredients that exhibit hair growth and tonic effects. Although scalp cosmetics, hair cosmetics, and hair growth agents containing these ingredients have been proposed, there is still a demand for plant-derived ingredients. [Prior art documents] [Patent documents]

[0004] [Patent Document 1] Japanese Patent Publication No. 64-083022 [Patent Document 2] Japanese Patent Application Publication No. 09-002935 [Patent Document 3] Japanese Patent Application Publication No. 09-077636 Summary of the Invention [Problem to be solved by the invention]

[0005] In view of the problems of the prior art, the present inventors have conducted extensive research to find new active ingredients derived from natural products. As a result, they have newly discovered that an extract of sage (Salvia officinalis) of the genus Salvia in the family Lamiaceae has the effect of activating hair papilla and outer root sheath cells, and inhibiting the production of lipid peroxides, and is useful as an ingredient for improving hair quality, maintaining scalp health, and promoting hair growth.

[0006] It has been known from Patent Documents 1 to 3 above that sage extract can be used as an ingredient in topical skin preparations, but it was not known that it has the effect of activating hair papilla cells, activating outer root sheath cells, and inhibiting the production of lipid peroxides, and is useful as an ingredient for improving hair quality, maintaining scalp health, and promoting hair growth. [Means for solving the problem]

[0007] The present invention is a hair composition containing a sage extract as an active ingredient. The present invention also relates to a scalp composition containing sage extract as an active ingredient. [Effects of the Invention]

[0008] According to the present invention, it is possible to provide a composition for hair or scalp which is highly safe for the living body and has excellent effects of improving hair quality, improving scalp health, and promoting hair growth. [Brief explanation of the drawings]

[0009] [Figure 1] FIG. 1 is a diagram showing the results of an evaluation test of the hair moisture-retaining function of the extract according to the present invention. DETAILED DESCRIPTION OF THE INVENTION

[0010] Preferred embodiments of the present invention will now be described in detail. The sage used in the present invention is Salvia officinalis, a plant of the genus Salvia in the family Lamiaceae, but the plant species is not particularly limited. The whole plant or above-ground parts are preferred. The whole plant or above-ground parts preferably include at least the flowers, leaves, and stems.

[0011] To prepare the extract, first, the part of the sage to be used is washed with water if necessary to remove foreign matter, and then either left as is or dried, and then shredded or crushed as necessary, and then brought into contact with an extraction solvent for extraction. Extraction can be carried out by contacting with the extraction solvent using a conventional method such as maceration, but supercritical extraction can also be used instead of maceration.

[0012] Examples of extraction solvents include water; lower alcohols such as methanol, ethanol, and propanol; polyhydric alcohols such as ethylene glycol, propylene glycol, 1,3-butylene glycol, and glycerin; esters such as ethyl acetate, butyl acetate, and methyl propionate; ketones such as acetone and methyl ethyl ketone; ethers such as ethyl ether and isopropyl ether; and hydrocarbon solvents such as n-hexane, toluene, and chloroform, and these may be used alone or in combination.

[0013] Among these extraction solvents, hydrophilic solvents such as water, lower alcohols, or polyhydric alcohols are preferred in the present invention from the viewpoints of the efficacy of the resulting extract, skin irritation, and the wide applicability to external skin preparations (cosmetics, quasi-drugs, external pharmaceuticals, etc.). Preferred examples of hydrophilic solvents include the use of water, lower alcohols (particularly ethanol), or polyhydric alcohols (particularly 1,3-butylene glycol and 1,3-propanediol) alone, or a mixed solvent of water and lower alcohols (particularly ethanol), or a mixed solvent of water and polyhydric alcohols (particularly 1,3-butylene glycol and 1,3-propanediol). Among these, water alone, a mixed solvent of water and ethanol, a mixed solvent of water and 1,3-butylene glycol, or a mixed solvent of water and 1,3-propanediol are particularly preferred.

[0014] When a mixed solvent is used, the mixing ratio is preferably in the range of 5:1 to 1:5 by volume, for example, when the mixed solvent is a mixture of water and ethanol, water and 1,3-butylene glycol, or water and 1,3-propanediol. The weight ratio of the part of the sage to be used to the extraction solvent is preferably 1:1 to 1:50.

[0015] When preparing the extract, the pH is not particularly limited, but is generally preferably in the range of 3 to 9. In this sense, if necessary, the extraction solvent may be blended with an alkalinity adjuster such as sodium hydroxide, sodium carbonate, or potassium hydroxide, or an acidity adjuster such as citric acid, hydrochloric acid, phosphoric acid, or sulfuric acid to adjust the pH to the desired level.

[0016] The extraction conditions, such as extraction temperature and extraction time, vary depending on the type and pH of the solvent used. For example, when water alone or a mixed solution of water and ethanol, 1,3-butylene glycol, or water and 1,3-propanediol is used as the solvent, the extraction temperature is preferably in the range of 0°C to 80°C, more preferably in the range of 20°C to 60°C, and the extraction time is preferably in the range of 1 to 168 hours (1 hour to 1 week), more preferably in the range of 1 to 120 hours (1 hour to 5 days).

[0017] If necessary, the extract may be subjected to a hydrolysis treatment prior to or in parallel with the extraction treatment of the present invention, which may improve the storage stability of the extract and allow the extract to be used more effectively as a cosmetic ingredient.

[0018] The extracts prepared as described above are mixed and generally adjusted to a pH of 3 to 8. The mixture may be used as an ingredient in a topical skin preparation as is, or may be used at a desired concentration by vacuum concentration, etc. The extract may also be dried by a conventional method such as spray drying.

[0019] Furthermore, it has been suggested that the combined use of the extract of the present invention with other hair growth ingredients, hair moisturizing ingredients, scalp moisturizing ingredients, or anti-inflammatory ingredients can achieve a synergistic effect of hair growth, hair moisturizing, scalp moisturizing, or anti-inflammatory. For example, ingredients that are expected to have the effects of hair growth, hair moisturizing, scalp moisturizing, or anti-inflammatory include minoxidil, cyproterone acetate, pentadecanoic acid glyceride, 6-aminobenzylpurine (cytopurine), adenosine, trans-3,4'-dimethyl 3-hydroxyflavanone (t-flavanone), Swertia japonica extract, Sophora root extract, hinokitiol, photosensitizers, pantothenic acid and its derivatives, vitamin E and its derivatives, nicotinic acid derivatives (nicotinamide, etc.), carpronium chloride, female hormones (ethinylestradiol, estrone, etc.), salicylic acid, dipotassium glycyrrhizinate, hinokitiol, and benzalkonium chloride. , isopropylmethylphenol, l-menthol, pyridoxine hydrochloride (vitamin B6), thioxolone, camphor, resorcinol, extract of Tamasakizurafuji root, bis-alkaloids obtained from Tamasakizurafuji, licorice extract, snail flower extract, chamomile extract, fermented royal jelly, fermented lotus seed, ginkgo extract, paldarco bark extract, gentian extract, ginseng extract, fermented soy milk, Salicornia extract, bamboo shoot skin extract, kudzu root extract, burdock extract, Mitsuishi Kami extract, clove extract, collagen, amino acids, and vitamins, and any one or more of these may be blended.

[0020] Furthermore, in addition to the above-mentioned components, compositions for hair or scalp containing the extract of the present invention (including cosmetics, quasi-drugs, and pharmaceuticals) can contain, as needed, ingredients typically used in hair growth and tonics and hair restoration agents (including quasi-drugs), such as oily ingredients, surfactants (synthetic and natural), moisturizers, thickeners, emulsifiers or emulsifier aids, preservatives, disinfectants, powder ingredients, UV absorbers, antioxidants, pigments, fragrances, and other physiologically active ingredients. Furthermore, there is no problem with combining the extract of the present invention with other physiologically active ingredients derived from natural products and incorporating them into compositions for external use on the skin.

[0021] Examples of oily components include olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice germ oil, coconut oil, palm oil, cocoa oil, meadowfoam oil, shea butter, tea tree oil, avocado oil, macadamia nut oil, bergamot oil, lavender oil, rose oil, bergamot oil, chamomile oil, and other plant-derived oils and fats such as squalane; vitamin A oil; animal-derived oils and fats such as mink oil and turtle oil; waxes such as beeswax, carnauba wax, rice wax, and lanolin; liquid paraffin, petrolatum, and paraffin wax. and squalane; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, and cis-11-eicosenoic acid; higher alcohols such as lauryl alcohol, cetanol, pantothenyl alcohol, and stearyl alcohol; synthetic esters and synthetic triglycerides such as isopropyl myristate, isopropyl palmitate, butyl oleate, 2-ethylhexyl glyceride, and higher fatty acid octyldodecyl (e.g., octyldodecyl stearate).

[0022] Examples of surfactants include nonionic surfactants such as polyoxyethylene alkyl ethers, polyoxyethylene fatty acid esters, polyoxyethylene sorbitan fatty acid esters, glycerin fatty acid esters, polyglycerin fatty acid esters, polyoxyethylene glycerin fatty acid esters, polyoxyethylene hydrogenated castor oil, and polyoxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkylphenyl ether sulfates, polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, polyoxyethylene Examples of surfactants that can be used include anionic surfactants such as ethylene alkyl phenyl ether phosphates; cationic surfactants such as quaternary ammonium salts, primary to tertiary fatty amine salts, trialkylbenzylammonium salts, alkylpyridinium salts, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salts, N,N-dialkylmorpholinium salts, and polyethylene polyamine fatty acid amide salts; and amphoteric surfactants such as N,N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N,N,N-trialkyl-N-alkyleneammoniocarboxybetaine, N-acylamidopropyl-N', N'-dimethyl-N'-β-hydroxypropylammoniosulfobetaine.

[0023] Examples of emulsifiers and / or emulsifying aids that can be blended include stevia derivatives such as enzyme-treated stevia, saponin or derivatives thereof, casein or its salts (sodium, etc.), sugar and protein complexes, sucrose or esters thereof, lactose, soybean-derived water-soluble polysaccharides, soybean-derived protein and polysaccharide complexes, lanolin or derivatives thereof, cholesterol, stevia derivatives (enzyme-treated stevia, etc.), silicates (aluminum, magnesium, etc.), carbonates (calcium, sodium, etc.), saponin and derivatives thereof, lecithin and derivatives thereof (hydrogenated lecithin, etc.), lactic acid bacteria-fermented rice, lactic acid bacteria-fermented germinated rice, lactic acid bacteria-fermented grains (wheat, beans, millet, etc.), etc.

[0024] Examples of moisturizing agents include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidone carboxylate, and the like, as well as sugars such as trehalose and raffinose, mucopolysaccharides (e.g., hyaluronic acid and its derivatives, hyaluronic acid fermentation liquid, chondroitin and its derivatives, heparin and its derivatives, etc.), elastin and its derivatives, collagen and its derivatives, collagen peptides, NMF-related substances, lactic acid, urea, higher fatty acid octyldodecyl, seaweed extract, estradiol, and various amino acids and their derivatives.

[0025] Examples of thickeners include components derived from brown algae, green algae, or red algae, such as alginic acid, agar, carrageenan, and fucoidan; polysaccharides such as pectin and aloe polysaccharide; gums such as tragacanth gum, locust bean gum, xanthan gum, and guar gum; cellulose derivatives such as carboxymethyl cellulose, hydroxyethyl cellulose, and hydroxypropyl cellulose; synthetic polymers such as carboxyvinyl polymers, alkyl-modified carboxyvinyl polymers, polyvinyl alcohol, polyvinylpyrrolidone, and acrylic acid-methacrylic acid copolymers; hyaluronic acid and its derivatives; polyglutamic acid and its derivatives, and polyacrylic acid.

[0026] Anti-inflammatory agents include allantoin, dipotassium glycyrrhizinate, monoammonium glycyrrhizinate, β-glycyrrhetinic acid, stearyl glycyrrhetinate, ε-aminocaproic acid, d-camphor, dl-camphor, zinc oxide, panthenol, pyridoxine hydrochloride, nicotinamide, and riboflavin or a derivative thereof.

[0027] Examples of antiseptics and disinfectants include urea; benzoic acid or its salts, parahydroxybenzoic acid esters such as methyl parahydroxybenzoate, ethyl parahydroxybenzoate, propyl parahydroxybenzoate, and butyl parahydroxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, benzalkonium chloride, salicylic acid, sodium salicylate, zinc pyrithione, benzalkonium chloride, ethanol, undecylenic acid, phenols, and alkane bromide. Examples include chrysoquinolinium, resorcinol, jamal (imidazolidinyl urea), isopropyl methylphenol, triclosan, trichlorocarbanide, trichlorohydroxydiphenol ether, hinokitiol, 1,2-pentanediol, propanediol, concentrated benzalkonium chloride solution 50, essential oils such as peppermint oil and eucalyptus oil, bark distillate, radish fermented liquid, ethanol derived from plants such as sugar cane and corn, or 1,3-butylene glycol.

[0028] Examples of cell activators include pantothenyl alcohol, menthol, dl-menthol, and γ-oryzanol.

[0029] Anti-acne agents include sulfur, salicylic acid or its salts, photosensitizer No. 201, pyridoxine dicaprylate, and the like.

[0030] Examples of powder components include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, nylon powder, polyethylene powder, silk powder, cellulose-based powder, powder of grains (rice, wheat, corn, millet, etc.), powder of beans (soybean, adzuki bean, etc.), etc.

[0031] Examples of ultraviolet absorbers include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and its derivatives, 2-ethylhexyl paramethoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5-sulfonate, 4-tert-butyl-4-methoxybenzoylmethane, 2-(2-hydroxy-5-methylphenyl)benzotriazole, urocanic acid, ethyl urocanate, and aloe extract.

[0032] Examples of antioxidants include butylhydroxyanisole, butylhydroxytoluene, propyl gallate, carotenoids such as astaxanthin, vitamin E and its derivatives (e.g., tocopherol acetate, tocopherol nicotinate), vitamin A and its derivatives (retinol palmitate, etc.), and the like.

[0033] Furthermore, it is also possible to use in combination components derived from natural products such as the following plants or microorganisms: collagen or hydrolysates thereof, yeast extracts or hydrolysates, lactic acid bacteria cultures, grasses, cruciferous plants, Theaceae plants, Rosaceae plants, Paeoniaceae plants, Rutaceae plants, Amaranthaceae plants, Zosteraea plants, Leguminosae plants, Asteraceae plants, Fabaceae plants, Malvaceae plants, Gentianaceae plants, Lamiaceae plants, Nelumbosacaceae plants, Cucurbitaceae plants, Araliaceae plants, Solanaceae plants, Bignoniaceae plants, Actinidiaceae plants, Mulberry plants, Iridaceae plants, Campanulaceae plants, Oleaceae plants, Actinidiaceae plants, Mulberry plants, Rhamnaceae plants, Orchidaceae plants, and Anacardiaceae plants. Examples of suitable extracts include extracts of one or more plants selected from the family Garcinia, Valenciaceae, Rutaceae, Myrtaceae, Liliaceae, Crassulaceae, Cupressaceae, Convolvulaceae, and Asparagaceae, or hydrolysates or fermented products thereof; extracts of one or more seaweeds selected from the family Laminaria, Mirrataceae, and Ulvulaceae, or hydrolysates or fermented products thereof; jellyfish (autolyzed products of moon jellyfish, Nomura's jellyfish, etc.); hydrolysates or fermented products of hyaluronic acid; and extracts of royal jelly, or hydrolysates or fermented products thereof.

[0034] As ingredients derived from grass plants, particularly preferred are rice leaf hydrolysate, rice extract hydrolysate, rice bran extract hydrolysate, germinated brown rice hydrolysate, rice fermentation liquid, sake lees extract derived from sake, bamboo shoot skin extract from Madake or Moso bamboo, and fermented Job's tears seed. Furthermore, as Brassicaceae plants, particularly preferred are extracts of seeds of Hakugai, Ogai, or Kokugai, or hydrolysates or fermented products thereof. Furthermore, as ingredients derived from Theaceae plants, particularly preferred are green tea (Yabukita, Samidori, Asahi, Goko, Ujimidori, Kyomidori, Ujihikari, Samidori, Benifuuki, etc.) and black tea (Darjeeling, Assam, Ceylon, Earl Grey, Honey Black Tea, etc.). As ingredients derived from Rosaceae plants, preferred are Damask rose flower extract, peach flower, leaf, or immature fruit extract, strawberry flower extract, and cherry blossom flower or leaf extract. Furthermore, as components derived from Paeoniaceae plants, extracts of peony root or flower, and peony flower are preferred. As components derived from Amaranthaceae plants, Salicornia extract is particularly preferred. As components derived from Zosteraea plants, extracts of Zostera marina or Zostera kohlrauss are particularly preferred. As components derived from Leguminosae plants, extracts of white soybeans or black soybeans or their hydrolysates, fermented soy milk, adzuki bean extract, red clover extract, and pueraria lobata root extract are particularly preferred. As components derived from Asteraceae plants, burdock root extract, sunflower sprout extract, Arctium gracilis extract, arnica extract, and chamomile flower extract are particularly preferred. As components derived from Malvaceae plants, fermented products of hibiscus, rose of sharon, or hibiscus are preferred. As components derived from Gentianaceae plants, gentian extract is preferred. As components derived from Lamiaceae plants, perilla extract and barberry fruit extract are preferred. As a component derived from a plant of the Nelumbo family, particularly preferred is a lotus flower or lotus seed extract or a fermented lotus seed product. As a component derived from a plant of the Cucurbitaceae family, particularly preferred is a loofah extract. As a component derived from a plant of the Araliaceae family, a ginseng extract or fermented product is preferred. As a component derived from a plant of the Solanaceae family, an extract of eggplant (long eggplant, water eggplant, rice eggplant, Kamo eggplant, etc.) is included. As a component derived from a plant of the Bignoniaceae family, a Pau d'Arco bark extract is preferred. As a component derived from a plant of the Actinidiaceae family, an immature kiwi extract is preferred.As components derived from plants of the Moraceae family, mulberry bark extract, mulberry fruit extract, and fig fruit or bark extract are preferred. As components derived from plants of the Rhamnaceae family, jujube fruit extract is preferred. Furthermore, as components derived from plants of the Iridaceae family, saffron is preferred. As components derived from plants of the Campanulaceae family, an extract or hydrolysate of Codonopsis globulus root is preferred. As components derived from plants of the Anacardiaceae family, mango fruit extract is particularly preferred. As components derived from plants of the Garciniaceae family, mangosteen fruit extract is particularly preferred. Furthermore, as components derived from plants of the Valenciaceae family, cherimoya fruit extract is preferred. As components derived from plants of the Rutaceae family, extracts of mandarin oranges, bergamot fruit extracts, grapefruit or banpeiyu fruit (including immature fruits), extracts containing flavonoids and their glycosides contained in plants such as grapefruit or hassaku, or Japanese pepper seed extract are preferred. As components derived from plants of the Liliaceae family, extracts of daylily, daylily, Casablanca lily, Madonna lily, or Japanese lily are preferred. As components derived from plants of the Crassulaceae family, an extract or fermented product of Rhodiola rosea is particularly preferred. As components derived from plants of the Oleaceae family, jasmine flower extract is particularly preferred. As plants of the Cupressaceae family, juniper fruit extract is particularly preferred. As components derived from plants of the Myrtaceae family, guava leaf extract is particularly preferred. As plants of the Orchidaceae family, an extract of Bletilla sieboldii root (white orchid) is particularly preferred. As components derived from plants of the Convolvulaceae family, sweet potato extract or a fermented product thereof, or sweet potato shochu lees extract or a fermented product thereof is particularly preferred. As components derived from seaweeds of the Laminaceae family, kelp extract is particularly preferred, as components derived from seaweeds of the Mirinaceae family, Katamenkirinsai extract is preferred, and as components derived from seaweeds of the Ulva family, Ulva pertusa extract is particularly preferred. As the component derived from Funoriaceae seaweed, Funori extract is particularly preferred.

[0035] The present invention will now be described in more detail with reference to Production Examples, Formulation Examples, and Test Examples, but the present invention is not limited thereto. In the following, all parts mean parts by weight, and all % mean % by weight.

[0036] Preparation Example 1. Preparation of extract The whole plant of sage (Lamiaceae, genus Salicylic Acid) was dried, and 90 g of the dried product was added with 1125 g of purified water and 1125 g of absolute ethanol, followed by stirring and extraction at 40°C for 2 hours. This was filtered to obtain 1825 g of a brown, transparent 50 w / w% sage ethanol extract (solid content concentration 0.9%). The obtained 50 w / w% sage ethanol extract was concentrated to obtain 440 g of a concentrate. Purified water and 1,3-butylene glycol were added to the concentrate, and this was filtered to obtain 1250 g of a brown, transparent sage extract (solid content concentration 1.02%).

[0037] Although an example of preparing a sage extract is shown in Preparation Example 1, the present invention is not limited to this. For example, the extraction solvent may be water alone, a mixed solvent of water and 1,3-butylene glycol, a mixed solvent of water and 1,3-propanediol, or a mixed solvent of water and ethanol.

[0038] Test Example 1. Dermal papilla cell activation effect test Human dermal papilla cells (HFDPC) were cultured in a 96-well microplate containing TMTPGM-250 medium at a concentration of 1 × 10 4 Cells were seeded at 1000 cells / well and pre-cultured at 37°C in 5.0% CO for 1 day. The extract from Preparation Example 1 was then added to the medium as a sample solution and cultured under the same conditions for an additional 3 days. The extract from Preparation Example 1 was added to the medium at final concentrations of 0.5%, 1.0%, and 2.0% relative to the total medium volume, resulting in three concentrations. The medium was then removed, 0.03% MTT was added, and the cells were incubated at 37°C for 1 hour. The resulting formazan was extracted with isopropanol, and the MTT value was measured at wavelengths of 570-630 nm using a microplate reader (Model 680, Bio-Rad). For comparison, a control was prepared by adding 2.0% 20% ethanol solution instead of the sample solution. The same procedure was repeated, and the relative MTT values ​​obtained with each sample to the control were calculated and used as the dermal papilla cell MTT activity rate (%). For comparison, a similar test was also carried out using glucose (100 mM) as a positive control.

[0039] The results of Test Example 1 are shown in Table 1. [Table 1] JPEG2025134242000002.jpg63112

[0040] As shown in Table 1, it was confirmed that the extract of Production Example 1 exerts a significant effect of activating dermal papilla cells, thereby making it possible to restore the health of dermal papilla cells that regulate the hair cycle. As a result, it is suggested that the extract has an effect of normalizing the hair cycle.

[0041] Test Example 2: Evaluation test of outer root sheath cell activation Human outer root sheath cells (HHORSCs) were placed in a 96-well microplate containing MSCM medium (ScienCell Research Laboratories) at 6 × 10 3 Cells were seeded per well and pre-cultured at 37°C and 5.0% CO for 1 day. Culture medium containing the extract from Preparation Example 1 as a sample solution was then added, and the cells were cultured under the same conditions for another 2 days. Three concentrations of the extract from Preparation Example 1 were used, resulting in final concentrations of 0.5%, 1.0%, and 2.0% of the total medium. The medium was then removed, 0.03% MTT was added, and the cells were incubated at 37°C for 1 hour. The resulting formazan was extracted with isopropanol, and the MTT values ​​were measured at wavelengths of 570-630 nm using a microplate reader (Model 450, Bio-Rad). For comparison, a control was prepared by adding 50% 1,3-butylene glycol instead of the sample solution. The same procedure was repeated, and the relative MTT values ​​obtained with each sample to the control were calculated, giving the outer root sheath cell MTT activity (%). In addition, to confirm whether the test system was functioning normally, a similar test was also carried out in the case where 100 mM glucose was added as a positive control instead of the sample solution.

[0042] The results of Test Example 2 are shown in Table 2. [Table 2] JPEG2025134242000003.jpg63112

[0043] As shown in Table 2, it was confirmed that the extract according to the present invention has a significant outer root sheath cell activation effect. Since the outer root sheath is a tissue that supports hair and promotes smooth hair growth, it is suggested that the extract according to the present invention has an effect of promoting hair growth.

[0044] Test Example 3: Evaluation test of inhibition of lipid peroxide production 1.0 mL of 0.5 M linoleic acid ethanol, 10 mL of 0.2 M phosphate buffer (pH 7.0), and 9.0 mL of ethanol were mixed and thoroughly stirred. 5.0 mL of the extract from Preparation Example 1 was added to this mixture and thoroughly stirred to prepare a sample solution. Three sample solutions were used, each with a final concentration (as a solution) of 0.5%, 1.0%, or 2.0% of the extract from Preparation Example 1 relative to the total volume. Immediately after preparation, each sample solution was left at 40°C for 1 day and 3 days. 0.1 mL of each solution was added, 4.7 mL of 75% ethanol, 0.1 mL of 30% ammonium thiocyanate solution, and 0.1 mL of 0.02 M ferrous chloride in 3.5% hydrochloric acid solution. After thorough mixing and stirring, the absorbance at 500 nm was measured 3 minutes later. Similar tests were also performed using a 20% ethanol solution as a control and α-tocopherol as a positive control. The rate of lipid peroxide production in this test system was expressed as a relative value of the absorbance in each sample-added group, with the absorbance of the control taken as 100.

[0045] The results of Test Example 3 are shown in Table 3. [Table 3] JPEG2025134242000004.jpg66166

[0046] As shown in Table 3, it was confirmed that the extract according to the present invention has a significant lipid peroxide suppressing effect. As a result, the present invention can provide a scalp composition that can suppress inflammation caused by oxidized lipids on the scalp.

[0047] Test Example 4: Evaluation test of hair quality improvement effect The hair cleansing compositions shown in Table 4 were prepared as samples for Test Example 4. A composition containing the extract of Production Example 1 was used as the composition of the present invention, and a composition not containing the extract of Production Example 1 was used as the comparative composition. JPEG2025134242000005.jpg83122

[0048] [Test method] First, a human hair bundle (9-10 g / hair) was subjected to a cleaning treatment in which the composition of the present invention was massaged into the entire hair, and then the composition of the present invention was thoroughly rinsed off with tap water. After cleaning, the hair was dried with warm air and brushed 10 times, and the degree of spreadability of the hair ends was measured. In addition, a human hair bundle (9-10 g / hair) was subjected to the same treatment using a comparative composition, and the degree of spreadability of the hair ends was also measured. Next, the human hair bundles that had been washed and dried using the above procedure were subjected to a high humidity treatment. Specifically, the human hair bundles were placed in a water-filled container in an incubator with a relative humidity of 90% or higher and left to stand for one hour. After one hour, the bundles were weighed over time in a laboratory (temperature: 28°C, humidity: 60%) to determine the amount of change from the initial weight.

[0049] The degree of spreading of human hair strands is shown in Table 5, and the weight change is shown in Figure 1. [Table 5] JPEG2025134242000006.jpg29108

[0050] As shown in Table 5 and Figure 1, it was confirmed that the composition of the present invention containing the extract of Production Example 1 suppresses frizziness at the ends of hair compared to the comparative composition. It was also confirmed that the composition of the present invention containing the extract of Production Example 1 has a superior ability to retain moisture in hair compared to the comparative composition.

[0051] Formulation example 1. Scalp care cosmetics [Ingredients] Part Adenosine 1.0 Extract of Preparation Example 1 1.0 1,3-butylene glycol 10.0 Phenoxyethanol 3.0 Ethanol 20.0 Purified water (enough to make the total volume 100 parts) The above ingredients were thoroughly mixed and stirred to obtain a scalp care cosmetic.

[0052] Formulation example 2: Scalp care cosmetics A scalp care cosmetic was obtained in the same manner as in Formulation Example 1, except that minoxidil was used instead of adenosine.

[0053] Formulation example 3: Scalp care cosmetics A scalp care cosmetic was obtained in the same manner as in Formulation Example 1, except that 6-benzylaminopurine was used instead of adenosine.

[0054] Formulation example 4: Hair cosmetics [Ingredients] Part l-menthol 0.8 Dipotassium glycyrrhizinate 0.1 Isopropylmethylphenol 0.1 Extract of Preparation Example 1 2.0 Soy lecithin 0.2 1,3-butylene glycol 2.0 1,2-Pentanediol 3.0 Ethanol 10.0 Tamasakizuzurafuji extract 0.01 Carrot extract 0.1 Swertia japonica extract 0.3 Bamboo shoot peel extract 3.0 Brown Algae Extract 3.0 Royal jelly fermented extract 3.0 Burdock Extract 3.0 Kudzu Root Extract 3.0 Citrus Fruit Peel Extract 3.0 Purified water (enough to make the total volume 100 parts)

[0055] Prescription example 5. Hair cream [Ingredients] Part Liquid paraffin 15.0 Vaseline 15.0 White Beeswax 2.0 Dipotassium glycyrrhizinate 0.1 Extract of Preparation Example 1 1.0 Swertia japonica extract 0.3 Tamasakizuzurafuji extract 0.01 Carrot extract 0.1 Brown algae extract 0.3 Carboxyvinyl polymer 0.1 Xanthan gum 0.1 Glycerin 5.0 1,3-butylene glycol 2.0 Polyoxyethylene hydrogenated castor oil 3.0 Hydrogenated lecithin 1.0 Lactic acid bacteria fermented rice 2.0 Chelating agent 0.1 Preservatives 0.1 Fragrance 0.1 Dye 0.01 Purified water (enough to make the total volume 100 parts)

[0056] Prescription example 6. Shampoo [Ingredients] Part Sodium N-cocoyl methyl taurate 10.0 Sodium polyoxyethylene (3) alkyl ether sulfate 20.0 Lauryl dimethylaminoacetic acid betaine 10.0 Coconut oil fatty acid diethanolamide 4.0 Methylparaben 0.1 Citric acid 0.1 Extract of Preparation Example 1 2.0 Dipotassium glycyrrhizinate 0.1 Nicotinamide 1.0 Collagen 1.0 Eelgrass extract 5.0 Salicornia extract 5.0 Kudzu Root Extract 5.0 Burdock extract 5.0 1,3-butylene glycol 2.0 1,2-Hexanediol 1.0 Purified water (enough to make the total volume 100 parts)

[0057] Example 7. Hair rinse [A component] part Polyoxyethylene (10) hydrogenated castor oil 1.0 Distearyldimethylammonium chloride 1.5 Stearyltrimethylammonium chloride 2.0 Glyceryl 2-ethylhexanoate 1.0 Cetyl alcohol 3.0 Stearyl Alcohol 1.0 Methylparaben 0.1 Extract of Preparation Example 1 2.0 Burdock extract 1.0 Pueraria lobata extract 1.0 1,3-butylene glycol 3.0 1,2-Pentanediol 1.0 Purified water (enough to make the total volume 100 parts)

Claims

1. A hair composition containing sage extract as an active ingredient.

2. A scalp composition containing sage extract as an active ingredient.

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