Highly stable and recombinant humanized elastin, genetically engineered bacterium expressing the elastin, and application therefor

Codon optimization of recombinant humanized elastin in a genetically engineered bacterium addresses stability issues, enhancing expression and efficacy in skincare products by improving stability and cell migration effects.

AU2025203768A1Pending Publication Date: 2026-07-16
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Patent Information

Authority / Receiving Office
AU · AU
Patent Type
Applications
Filing Date
2025-05-21
Publication Date
2026-07-16

AI Technical Summary

Technical Problem

Existing recombinant humanized elastin synthesized through prokaryotic expression systems suffers from poor stability and is prone to degradation, leading to reduced efficacy in applications such as skincare products.

Method used

A codon-optimized nucleotide sequence for recombinant humanized elastin is introduced into Escherichia coli to construct a genetically engineered bacterium, enhancing the stability and expression level of the elastin, and increasing its migration effect on HSF cells and gene expression levels of anti-aging and anti-wrinkle related genes.

Benefits of technology

The codon-optimized recombinant humanized elastin demonstrates improved stability, higher expression levels, and increased cell migration rates, along with enhanced gene expression of anti-aging and anti-wrinkle related genes, indicating better performance in skincare applications.

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Abstract

The present disclosure provides a highly stable and recombinant humanized elastin, a genetically engineered bacterium expressing the elastin, and an application therefor, and belongs to the technical field of connective tissue peptides. A nucleotide sequence of the recombinant humanized elastin is as shown in SEQ ID NO.1; and an amino acid sequence is as shown in SEQ ID NO.4. The present disclosure performs codon optimization on the nucleotide sequence of the humanized elastin and then transfer the nucleotide sequence into Escherichia coli to construct a genetically engineered bacterium expressing the recombinant humanized elastin, which can increase an expression level of the recombinant humanized elastin, improve stability of the recombinant humanized elastin, increase a migration rate of the recombinant humanized elastin to HSF cells, and increase gene expression levels of h-ELN, h-COL1A1, and h-COL3A1 in HSF cells. 20 25 20 37 68 21 M ay 2 02 5 A B S T R A C T 2 0 2 5 2 0 3 7 6 8 2 1 M a y 2 0 2 5 1 / 2 FIG. 1 FIG. 2 1 / 2 M 1 2 3 4 kDa 180 130 100 70 55 40 35 25 15 FIG. 1 Oh 24h HSF Oh 24h NC rhELN-optimization 1 rhELN-optimization 2 rhELN-pre-optimization FIG. 2 20 25 20 37 68 21 M ay 2 02 5 k D a 1 8 0 2 0 2 5 2 0 3 7 6 8 2 1 M a y 2 0 2 5 5 5 3 5 2 5 F I G . 1 O h 2 4 h H S F O h 2 4 h N C
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