Cellulomonas fimi DA 8 bacterial strain as well as acquiring method and application thereof

Abstract

The invention relates to a cellulomonas fimi DA 8 bacterial strain as well as an acquiring method and application thereof. The bacterial strain has the capability of producing pectinase and hemicellulase and has flax degumming activity. The cellulomonas fimi bacterial strain screened by the method has the advantages of large enzyme yield, complete enzyme system, no toxicity, rough culturing condition and the like; after used for flax degumming, the composite degumming enzyme solution not only greatly shortens a degumming period, but also reduces the cost, the fiber splitting degree is improved, and the degumming efficiency reaches 95%. The degumming method requires basically the same equipment required in the traditional warm water degumming method so that the method is especially suitable for improving a process on the basis of the traditional equipment in old enterprises; the cellulomonas fimi bacterial strain screened with the method not only can be used for flax raw-stem degumming, roving scouring, flax fabric biological pretreatment, but also can be used for bast fiber degumming and biological pretreatment of bast fiber plants, such as ramie, jute, hemp and the like.

Description

technical field

[0001] The invention belongs to the field of cellulomonas strains and applications thereof, in particular to a cellulomonas DA8 strain and its obtaining method and application. Background technique

[0002] Because of its "green health care" characteristics, linen textiles are favored by consumers and are one of the most popular textile products in the international market. Fiber flax is grown in more than 20 countries and regions in the world. In 2004, the planting area of ​​fiber flax in my country has reached 160,000 mu, ranking first in the world. Heilongjiang Province is the main production base of flax for fiber in my country, and its planting area and output account for more than 70% of the country's total. However, the annual output of flax fiber in my country is only 40,000-50,000 tons, and the gap is more than 50%. The deficiencies of flax degumming technology lead to poor fiber quality, low fiber length and carding rate, weak strength, and poor ...

Examples

Embodiment 1

[0057] Screening and Obtaining of Flax Degumming Strains

[0058] (1) Sampling rotting seaweed from the Zhoushan sea area of ​​the East China Sea, first put the seaweed and the enrichment medium at a ratio of 1g: 20ml in the enrichment medium and culture it at room temperature for 30 days, then take 0.1ml of the enrichment medium and apply it to the separation medium Culture medium, static culture at 37°C for 1 to 4 days, obtained from the wild-type degumming dominant strain;

[0059] Among them, the formula of the enrichment medium is: 5 g of flax powder, 200 ml of sterile water, and the pH is natural; the formula of the separation medium is: 5 g of flax powder, (NH 4 ) 2 SO 4 5g, K 2 HPO 4 1g, MgSO 4 0.5g, KCl 0.5g, FeSO 4 0.01, 20g agar, 1000mL water, adjust the pH to 9 with NaOH;

[0060] (2) Inoculate 4 loops of the bacterial strain obtained in step (1) into pectin nutrient medium (petri dish diameter 10cm), cultivate at 28°C for 72h, after the formation of col...

Embodiment 2

[0079]In order to enable the strain to maintain a stable high-yielding characteristic, beef extract peptone medium is used as the slant preservation medium. The raw materials and preparation methods of the medium are: 0.5g beef extract, 1g peptone, and 0.5g sodium chloride (pH7.4-7.6) , add water to 100ml constant volume, culture overnight at 37°C 200rpm, add 100ml of freezing buffer; among them, the freezing buffer consists of 0.0627g of potassium dihydrogen phosphate, 0.0177g of dipotassium hydrogen phosphate, 0.0588g of sodium citrate, sulfuric acid heptahydrate Magnesium 0.02645g, glycerin 10ml, add water to volume to 100ml; divide nutrient broth medium (beef extract 0.3g, peptone 1g, sodium chloride 0.5g, add water to 100ml volume, pH7.4-7.6) In 250mL containers, 50mL in each container, after sealing and bandaging, sterilize at 121°C for 20min; after cooling, insert the bacteria loop cultured on a slant for 24h, and shake the flask at a speed of 200r / min and a temperature ...

Embodiment 3

[0089] The flax degumming process is as follows:

[0090] Put 5g of the original flax stem into a 250ml conical flask, divide the flax flax and the fermented bacteria liquid into the conical flask at a bath ratio of 1:20, the liquid volume is 100ml, degumming in a shaker at 40°C and 200rpm for 1 day , 2 days and 4 days; when the degumming time is up, remove the degumming solution, boil in a boiling water bath for 5 minutes, rinse with tap water several times to remove bacteria on the linen, and stop degumming.

[0091] Through the observation of degummed flax, it was found that the flax fibers were obviously dispersed after 1 day, and the color of the fermentation broth became dark and turbid; after 2 days of degumming, the original bundled flax was completely dispersed into fibers, and the fermentation broth was obviously thickened; after 4 days of degumming, the degumming rate of flax reached more than 90 percent.

technical field

[0001] The invention belongs to the field of cellulomonas strains and applications thereof, in particular to a cellulomonas DA8 strain and its obtaining method and application. Background technique

[0002] Because of its "green health care" characteristics, linen textiles are favored by consumers and are one of the most popular textile products in the international market. Fiber flax is grown in more than 20 countries and regions in the world. In 2004, the planting area of ​​fiber flax in my country has reached 160,000 mu, ranking first in the world. Heilongjiang Province is the main production base of flax for fiber in my country, and its planting area and output account for more than 70% of the country's total. However, the annual output of flax fiber in my country is only 40,000-50,000 tons, and the gap is more than 50%. The deficiencies of flax degumming technology lead to poor fiber quality, low fiber length and carding rate, weak strength, and poor ...

Examples

Embodiment 1

[0057] Screening and Obtaining of Flax Degumming Strains

[0058] (1) Sampling rotting seaweed from the Zhoushan sea area of ​​the East China Sea, first put the seaweed and the enrichment medium at a ratio of 1g: 20ml in the enrichment medium and culture it at room temperature for 30 days, then take 0.1ml of the enrichment medium and apply it to the separation medium Culture medium, static culture at 37°C for 1 to 4 days, obtained from the wild-type degumming dominant strain;

[0059] Among them, the formula of the enrichment medium is: 5 g of flax powder, 200 ml of sterile water, and the pH is natural; the formula of the separation medium is: 5 g of flax powder, (NH 4 ) 2 SO 4 5g, K 2 HPO 4 1g, MgSO 4 0.5g, KCl 0.5g, FeSO 4 0.01, 20g agar, 1000mL water, adjust the pH to 9 with NaOH;

[0060] (2) Inoculate 4 loops of the bacterial strain obtained in step (1) into pectin nutrient medium (petri dish diameter 10cm), cultivate at 28°C for 72h, after the formation of col...

Embodiment 2

[0079]In order to enable the strain to maintain a stable high-yielding characteristic, beef extract peptone medium is used as the slant preservation medium. The raw materials and preparation methods of the medium are: 0.5g beef extract, 1g peptone, and 0.5g sodium chloride (pH7.4-7.6) , add water to 100ml constant volume, culture overnight at 37°C 200rpm, add 100ml of freezing buffer; among them, the freezing buffer consists of 0.0627g of potassium dihydrogen phosphate, 0.0177g of dipotassium hydrogen phosphate, 0.0588g of sodium citrate, sulfuric acid heptahydrate Magnesium 0.02645g, glycerin 10ml, add water to volume to 100ml; divide nutrient broth medium (beef extract 0.3g, peptone 1g, sodium chloride 0.5g, add water to 100ml volume, pH7.4-7.6) In 250mL containers, 50mL in each container, after sealing and bandaging, sterilize at 121°C for 20min; after cooling, insert the bacteria loop cultured on a slant for 24h, and shake the flask at a speed of 200r / min and a temperature ...

Embodiment 3

[0089] The flax degumming process is as follows:

[0090] Put 5g of the original flax stem into a 250ml conical flask, divide the flax flax and the fermented bacteria liquid into the conical flask at a bath ratio of 1:20, the liquid volume is 100ml, degumming in a shaker at 40°C and 200rpm for 1 day , 2 days and 4 days; when the degumming time is up, remove the degumming solution, boil in a boiling water bath for 5 minutes, rinse with tap water several times to remove bacteria on the linen, and stop degumming.

[0091] Through the observation of degummed flax, it was found that the flax fibers were obviously dispersed after 1 day, and the color of the fermentation broth became dark and turbid; after 2 days of degumming, the original bundled flax was completely dispersed into fibers, and the fermentation broth was obviously thickened; after 4 days of degumming, the degumming rate of flax reached more than 90 percent.

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