Microfluidic device and method for isolating target using same
By designing an inverted funnel array and a counter flow guidance unit in the microfluidic device, the problem of low target cell capture rate in the existing technology is solved, and efficient separation and collection of target cells in blood are achieved.
CN102947701AActive Publication Date: 2013-02-27CYTOGEN CORP
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Patent Information
- Application Number
- CN201180029403.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Priority Date
- 2010-04-15
- Filing Date
- 2011-04-15
- Publication Date
- 2013-02-27
- Estimated Expiration
- 2031-04-15
AI Technical Summary
Technical Problem
Existing microfluidic devices have low capture rates when capturing target cells from blood and are not suitable for separation and analysis of large volumes of blood.
Method used
A microfluidic device is designed, including a housing with an inlet and an outlet, multiple funnel arrays arranged orthogonally along the sample flow direction, and a counterflow guide unit. The configuration of the funnel array is inverted to improve the capture and capture of target cells. separation efficiency.
Benefits of technology
Effectively captures and separates target cells in blood, is suitable for easily collecting cells from large amounts of blood, and improves capture rate and separation efficiency.
✦ Generated by Eureka AI based on patent content.
Abstract
Disclosed are a microfluidic device for isolating targets from a sample and a method for isolating targets using same. The microfluidic device of the present invention comprises a case, a first capture array, and a second capture array. The case has a channel formed therein, the channel having an inlet for introducing a sample having a plurality of different types of targets, and an outlet for discharging the sample. The first capture array is provided upstream of the channel, and includes a plurality of first funnels arranged along the direction orthogonal to the flow direction of the sample so as to capture the plurality of different types of targets.; The second capture array is provided downstream of the first capture array, and includes a plurality of second funnels arranged along the direction orthogonal to the flow direction of the sample so as to capture the plurality of different types of targets. The targets captured on the first and second funnels are discharged to the outside of the case and collected by turning over and positioning the case and supplying a solution thereto.
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