Methods for extracting quercetin derivatives from tea leaves

Through ethanol solution extraction and multi-chromatography separation technology, high-purity quercetin derivatives were successfully extracted from Camellia oleifera leaves, which solved the problems of insufficient extraction efficiency and purity in the existing technology and achieved efficient quercetin derivatives. material extraction.

CN105924484BInactive Publication Date: 2019-02-19CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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Patent Information

Application Number
CN201610283456.2
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2016-04-29
Publication Date
2019-02-19
Estimated Expiration
Not applicable · inactive patent

AI Technical Summary

Technical Problem

It is difficult to effectively extract quercetin derivatives from Camellia oleifera leaves, especially high-purity quercetin-3-rhamnoside, quercetin-3-O-β-D-glucoside and quercetin- 3-O-β-D-galactopyranoside.

Method used

Using ethanol solution extraction method, combined with D101 macroporous adsorption resin and silica gel chromatography separation technology, quercetin derivatives are gradually separated and extracted through gradient elution and chromatography analysis, using DAC chromatography column and semi-preparative liquid chromatography separation technology to achieve Quercetin-3-rhamnoside, quercetin-3-O-β-D-glucoside and quercetin-3-O-β-D-galactoside are efficiently extracted from Camellia oleifera leaves.

Benefits of technology

Achieved the extraction of high purity (≥90%) and high yield (above 96.4mg/kg) quercetin-3-rhamnoside and a mixture of CO-8a and CO-8b above 30.3mg/kg, suitable for Camellia oleifera leaves of different species, especially common Camellia oleifera.

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Abstract

The invention provides a method for extracting quercetin derivatives from camellia oleifera leaves. The method comprises the following steps: camellia oleifera leaves are subjected to lixiviation; D101 is adopted as a column packing, and a crude extract liquid is eluted with a 75-85% ethanol solution; the elution liquid is subjected to silica gel chromatography separation, and fraction segments containing quercetin-3-rhamnoside and quercetin glucoside (or galactoside) are combined; DAC column chromatography separation is carried out, and a quercetin-3-rhamnoside fraction is collected; fraction segments containing quercetin glucoside (or galactoside) are combined, and the combination is named as a second sequential component; the second sequential component is subjected to silica gel chromatography, and fraction segments containing quercetin glucoside (or galactoside) are combined; semi-preparative liquid chromatography is carried out, and a fraction containing quercetin glucoside (or galactoside) is collected. According to the invention, for a first time, three quercetin derivatives are successfully extracted from camellia oleifera leaves. The yield and product purity are high.
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