A method for degrading tobacco stalk lignin

A tobacco stalk and lignin technology, applied in the field of bioengineering, can solve problems such as limiting the popularization and application of biological treatment methods, lignin loss, etc.

CN109880837BActive Publication Date: 2022-02-11ZHENGZHOU UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Publication Date
2022-02-11

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Abstract

The invention discloses a method for degrading tobacco stalk lignin, comprising: constructing a peroxidase gene expression vector containing rice GRP signal peptide gene, GFP gene and SHP gene, and introducing it into Agrobacterium LBA4404 to construct a mature Transgenic tobacco plants; finally hydrogen peroxide was used to degrade lignin in mature transgenic tobacco plants. The peroxidase gene expression vector constructed by the present invention can make the endogenous SHP enzyme of the crop straw overexpress when the crop grows normally, and make it fully contact with the cell wall components such as straw lignin along with the normal growth and development of the crop , does not affect the lignin content in the crop stalks, and after the crop stalks are harvested, the mature stems are harvested, and the lignin content of the transgenic plant stems can be reduced by 3%‑5 after being treated with a 5% hydrogen peroxide solution by volume fraction %, the method of the present invention has a good application prospect in the degradation of crop straw lignin.
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Description

technical field

[0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for degrading tobacco stalk lignin. Background technique

[0002] Lignocellulosic resources are abundant renewable energy substances in nature. As the main carrier of biomass energy, its research and application have been widely concerned at home and abroad. Lignin is one of the three main components of the plant cell wall. It surrounds or binds in the cellulose framework, which can enhance the mechanical strength of the plant body, and at the same time facilitate the water transport of the tissue and resist the adverse invasion of the external environment. The complexity of its structure and the stable form in the body make it difficult to be degraded, industrially separated or transformed, and it has become the primary barrier for the resource utilization of lignocellulose.

[0003] One of the key technologies to improve the quality of corn and othe...

Examples

Embodiment Construction

[0048] The specific embodiments of the invention will be described in detail below, but it should be understood that the protection scope of the present invention is not limited by the specific embodiments. The test methods for which specific conditions are not indicated in the following examples are usually in accordance with conventional conditions, or in accordance with the conditions suggested by each manufacturer. In addition, various enzymes used in the present invention and the following examples are commercially available.

[0049] The present invention uses genetic engineering technology, based on the commonly used tobacco plant expression vector, uses a GRP signal peptide sequence in the rice genome, and converts a coding sequence of soybean hull peroxidase (Soybean hull peroxidase, SHP) gene derived from soybean It is fused with the coding sequence of the green fluorescent protein GFP gene to construct a directional fusion expression vector to promote soybean hull p...