Light Metering Device

Abstract

The present invention provides a photometric device, comprising a photomultiplier tube, a reaction container and a liquid injection needle, the photomultiplier tube comprising a detection body and a connection part, the detection body having a photometric window, the connection part having a cavity, and the cavity being connected to the photometric window, the connection part being used to be detachably connected to the reaction container, the reaction container having an inner cavity with an opening at one end, the inner cavity being used to accommodate a luminous object to be detected, when the photomultiplier tube is connected to the reaction container, the cavity being connected to the photometric window and the inner cavity, the liquid injection needle being arranged on the connection part, the first end of the liquid injection needle being located in the cavity and the projection of the first end in the direction of the inner cavity being located in the inner cavity, the second end of the liquid injection needle being located outside the connection part, and being used to introduce the excitation liquid provided by the liquid injection device into the inner cavity of the reaction container to react with the luminous object contained in the inner cavity to make the luminous object emit photons, the photomultiplier tube being used to simultaneously collect photons through the photometric window to detect the content of the luminous object. The light measuring device of the present invention has a simple structure and high detection efficiency.

Description

Technical Field

[0001] The present invention relates to the field of medical detection, and in particular to a light measuring device. Background Art

[0002] Chemiluminescent immunoassay technology has gained widespread application in biological testing due to its high accuracy and ease of use. Chemiluminescent immunoassay utilizes a series of reactions in which a chemiluminescent substance forms an excited intermediate. When this excited intermediate returns to a stable ground state, it simultaneously emits photons. The luminescent signal is then used to measure the analyte content. Therefore, chemiluminescent immunoassay instruments must incorporate a photometric mechanism. Existing photometric mechanisms are complex and inefficient. Summary of the Invention

[0003] The present invention provides a light measuring device, which has a simple structure and high detection efficiency.

[0004] The present invention provides a photometric device, which includes a photomultiplier tube, a reaction container, and an injection needle. The photomultiplier tube includes a detection body and a connecting part. The detection body has a photometric window. The connecting part has a cavity with two ends open, and the cavity is connected to the photometric window. The connecting part is used to be detachably connected to the reaction container. The reaction container has an inner cavity open at one end, and the inner cavity is used to accommodate a luminescent substance to be detected. When the photomultiplier tube is connected to the reaction container, the cavity is connected to the photometric window and the inner cavity. The injection needle is arranged on the connecting part. The first end of the injection needle is located in the cavity, and the projection of the first end in the direction of the inner cavity is located in the inner cavity. The second end of the injection needle is located outside the connecting part. The injection needle is used to introduce excitation liquid provided by the injection device into the inner cavity of the reaction container to react with the luminescent substance accommodated in the inner cavity to cause the luminescent substance to emit photons. The photomultiplier tube is used to simultaneously collect the photons to detect the content of the luminescent substance.

[0005] Among them, the connecting part is a quick-release connector, the injection needle is arranged through the side wall of the quick-release connector, one end of the quick-release connector is used to connect with the detection body, and the other end is used to connect with the reaction container. The detection body and the reaction container are detachably connected through the quick-release connector.

[0006] The injection needle includes a first section and a second section, the first section passes through the side wall of the quick-release connector, the second section is bent and connected to the first section, and at least a portion of the second section is located in the inner cavity.

[0007] The two ends of the first section are respectively located on the inner and outer sides of the quick-release joint, the end of the first section located on the outer side of the quick-release joint is the second end of the injection needle, and the second end is provided with a hollow adapter. The injection device includes an injection tube and a liquid storage tank. The adapter is used to be connected to the injection tube, and the injection tube is connected to the liquid storage tank for storing the excitation liquid. The excitation liquid in the liquid storage tank is injected into the inner cavity of the reaction container through the injection tube, the adapter and the injection needle.

[0008] In which, the quick-release connector includes a first sub-connector and a second sub-connector that are connected to each other, the first sub-connector and the second sub-connector are detachably connected, the end of the first sub-connector facing away from the second sub-connector is connected to the detection body, and the end of the second sub-connector facing away from the first sub-connector is connected to the reaction container, the first sub-connector and the second sub-connector cooperate to seal the photometric device to form a closed cavity including the cavity and an inner cavity connected to the cavity, and align the photometric window of the detection body with the inner cavity of the reaction container.

[0009] The first sub-connector and the second sub-connector cooperate to form a mounting hole that integrally passes through the side wall of the quick-release connector. The mounting hole is connected to the cavity. At least a portion of the first section is located in the mounting hole, and both ends of the first section extend outside the mounting hole.

[0010] The first sub-connector has a first mounting hole that passes through the side wall of the first sub-connector, and the second sub-connector has a second mounting hole that passes through the side wall of the second sub-connector. When the first sub-connector and the second sub-connector are connected in a mating relationship, the first mounting hole and the second mounting hole are directly connected to each other, thereby forming the mounting hole that passes through the side wall of the quick-release connector as a whole.

[0011] The second sub-connector has a first end face, which is the end face of the second sub-connector facing away from the first sub-connector. A first protrusion and a second protrusion are arranged on the first end face around the cavity. The first protrusion and the second protrusion cooperate to form a first groove, which is used to accommodate at least part of the reaction container.

[0012] The reaction container has a second end face, on which a third protrusion and a fourth protrusion are arranged around the inner cavity. The third protrusion and the fourth protrusion cooperate to form a second groove, and the second groove is used to accommodate at least part of the second sub-connector.

[0013] In which, when the first protrusion is inserted into the second groove and the end of the first protrusion facing away from the first end face is abutted against the second end face, and the fourth protrusion is inserted into the first groove and the end of the second protrusion facing away from the second end face is abutted against the first end face, there is a gap between the circumferential side surface of the first protrusion and the groove side wall of the second groove, and there is also a gap between the circumferential side surface of the fourth protrusion and the groove side wall of the first groove.

[0014] In which, the light measuring device also has a light-shielding part, which includes a fixing part and a light-shielding plate. The fixing part is located on one side of the detection body. The light-shielding plate includes a first sub-light-shielding plate and a second sub-light-shielding plate. The first sub-light-shielding plate is rotatably mounted on the bottom surface of the fixing part, and the second sub-light-shielding plate is bent and connected to the first sub-light-shielding plate, and the second light-shielding plate is located on the side of the quick-release joint away from the detection body. The first light-shielding plate is rotated by an external force, driving the second sub-light-shielding plate to cover or expose the quick-release joint.

[0015] The photometric device of the present invention connects the photomultiplier tube to the reaction vessel, eliminating the need for a reaction vessel transfer mechanism. The photometric window of the detection body can be connected to the inner cavity of the reaction vessel through the cooperation of the connecting portion, thereby simplifying the structure of the photometric device. Furthermore, an injection needle is provided on the connecting portion, with the first end of the injection needle positioned within the cavity and the second end positioned outside the connecting portion, providing a passage for introducing the excitation liquid in the injection device into the inner cavity of the reaction vessel. The excitation liquid causes the luminescent substance to emit photons under the excitation of the excitation liquid. The photomultiplier tube then collects the number of photons generated during the reaction process through the photometric window in real time, and calculates the luminescent substance content based on the number of photons to complete the detection process. The detection process allows photometry to be performed simultaneously with the addition of the excitation liquid, simplifying the detection process and improving detection efficiency. Furthermore, because the luminescence process is brief, performing photometry simultaneously with the injection of the excitation liquid can improve detection accuracy and avoid the occurrence of large errors. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the technical solution of the present invention, the following is a brief introduction to the drawings required for use in the implementation. Obviously, the drawings described below are only some implementation methods of the present invention. For ordinary technicians in this field, other drawings can be obtained like these drawings without paying any creative work.

[0017] Figure 1 is a schematic diagram of a light measuring device provided in an embodiment of the present application;

[0018] Figure 2 yes Figure 1A schematic cross-sectional view of a light measuring device is provided;

[0019] Figure 3 yes Figure 1 A schematic diagram of the detection body of the provided photomultiplier tube;

[0020] Figure 4 yes Figure 1 A schematic diagram of the quick-release connector at one angle is provided;

[0021] Figure 5 yes Figure 1 A schematic diagram of the quick-release connector from another angle is provided;

[0022] Figure 6 yes Figure 1 A schematic cross-sectional view of the provided injection needle;

[0023] Figure 7 yes Figure 1 A cross-sectional diagram of the provided quick-release connector;

[0024] Figure 8 yes Figure 1 A schematic diagram of the provided reaction vessel;

[0025] Figure 9 yes Figure 1 A schematic diagram of the assembly of the detection body and the quick-release connector is provided, wherein the second light shielding plate exposes the quick-release connector.

[0026] Figure 10 yes Figure 1 A schematic diagram of the assembly of the detection body and the quick-release connector is provided, wherein the second light shielding plate covers the quick-release connector.

[0027] Figure 11 yes Figure 1 A partial cross-sectional view of the light measuring device is provided. DETAILED DESCRIPTION

[0028] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. All other embodiments obtained by ordinary technicians in this field based on the embodiments of the present invention without making any creative efforts shall fall within the scope of protection of the present invention.

[0029] See also Figure 1 and Figure 2The present invention provides a photometric device 100, which includes a photomultiplier tube 10, a reaction container 20 and an injection needle 40. The photomultiplier tube 10 includes a detection body (not shown) and a connecting portion (not shown). The detection body has a photometric window 11, and the connecting portion has a cavity with two ends open (not shown), and the cavity is connected to the photometric window 11. The connecting portion is used to be detachably connected to the reaction container 20. The reaction container 20 has an inner cavity 21 with one end open, and the inner cavity 21 is used to accommodate a luminous object to be detected. When the detection body is connected to the reaction container 20 through the connecting portion, The cavity is connected to the photometric window 11 and the inner cavity 21, the injection needle 40 is arranged on the connecting part and the first end 41 of the injection needle 40 is located in the cavity and the projection of the first end 41 in the direction of the inner cavity 21 is located in the inner cavity 21, the second end 42 of the injection needle 40 is located on the outside of the connecting part, and is used to introduce the excitation liquid provided by the injection device (not shown) into the inner cavity 21 of the reaction container 20 to react with the luminescent object contained in the inner cavity 21 to make the luminescent object emit photons, and the photomultiplier tube 10 is used to simultaneously collect the photons through the photometric window 11 to detect the content of the luminescent object.

[0030] The photometric device 100 is provided with a reaction vessel 20 to accommodate the reaction between the luminescent substance to be detected and the excitation solution. By utilizing the reaction between the luminescent substance and the excitation solution, the luminescent substance releases a large amount of free energy when oxidized in an alkaline medium, forming an excited intermediate. When this excited intermediate returns from its lowest vibrational energy level to a stable ground state, each vibrational energy level radiates and generates energy. The excess energy is emitted as photons, thereby generating luminescence. Simultaneously, a photomultiplier tube 10 in the photometric device 100 collects these photons to measure the light quantum yield. In this embodiment, the photometric device 100 can be used in a chemical immunoassay tester, so that the light quantum yield collected by the photometric device 100 can be converted into concentration units of the substance being tested by the relevant computer system of the chemical immunoassay tester to detect the content of the luminescent substance. In this embodiment, the luminescent substance is formed by directly labeling the antigen or antibody with a marker. The marker is an acridinium ester that generates an excited intermediate when excited by the excitation solution. As will be understood, the acridinium ester has luminescent properties.

[0031] By connecting the photomultiplier tube 10 to the reaction container 20, the photometric window 11 of the detection body can be connected to the inner cavity 21 of the reaction container 20 under the cooperation of the connecting part without setting up a reaction container transfer mechanism, thereby simplifying the structure of the photometric device 100, and by setting an injection needle 40 on the connecting part, and making the first end 41 of the injection needle 40 located in the cavity 21, and the second end 42 of the injection needle 40 located on the outside of the connecting part, a channel is provided to introduce the excitation liquid in the injection device into the inner cavity 21 of the reaction container 20, the excitation liquid reacts with the luminescent substance contained in the inner cavity 21, so that the luminescent substance emits photons, and then the photomultiplier tube 10 collects the number of photons generated in the reaction process in real time through the photometric window 11, and calculates the luminescent substance content based on the number of photons to complete the detection process. The detection process can realize photometry while adding the excitation liquid. On the one hand, it simplifies the detection process and improves the efficiency of detection. On the other hand, because the luminescence process is short, completing the photometry while injecting the excitation liquid can improve the accuracy of the detection and avoid the occurrence of large errors.

[0032] Please also refer to Figure 3 The photomultiplier tube 10 is suitable for detecting weak light signals and can achieve photon counting level detection. Therefore, the photomultiplier tube 10 can detect photons emitted by the luminescent substance to be detected in the reaction vessel 20, and detect the concentration of the luminescent substance to be detected by the number of collected photons, with high measurement accuracy and accurate measurement results. In this embodiment, the detection body (not shown) includes a housing 12 and a bottom plate 13 that encapsulates the housing 12. The bottom plate 13 is stepped, that is, one end of the bottom plate 13 is elevated relative to the other end. In this embodiment, the elevated end is used as the mounting end 131 as an example. The mounting end 131 has a mounting surface 132 and a side surface 133 disposed adjacent to the mounting surface 132. The side surface 133 is the surface of the mounting end 131 that is close to the housing 12. The photometric window 11 is disposed on the mounting surface 132 and is used to collect the photons for measurement. In this embodiment, the photometric window 11 is circular to facilitate communication with the cavity of the connecting portion. In other embodiments, the light measuring window 11 may also be in other shapes such as a rectangle, which is not limited here.

[0033] In this embodiment, the connecting portion is a quick-release connector 30, and the injection needle 40 is provided through the side wall of the quick-release connector 30. One end of the quick-release connector 30 is used to connect to the detection body, and the other end is used to connect to the reaction vessel 20. The detection body and the reaction vessel 20 are detachably connected via the quick-release connector 30. In other embodiments, the connecting portion can be fixedly connected to the detection body, that is, the connecting portion is formed by extending from the housing 12 of the detection body.

[0034] Please also refer to Figure 2 、 Figure 4 and Figure 5 The quick-release connector 30 has a cavity 31 connected at both ends, that is, the quick-release connector 30 is a hollow structure, which includes a first sub-connector 32 and a second sub-connector 33 connected to each other. The end of the first sub-connector 32 facing away from the second sub-connector 33 is connected to the detection body, and the end of the second sub-connector 33 facing away from the first sub-connector 32 is connected to the reaction container 20. The first sub-connector 32 and the second sub-connector 33 cooperate to seal the light measuring device 100, forming a closed cavity (not marked in the figure) including the cavity 31 and the inner cavity connected to the cavity 21, and aligning the light measuring window 11 of the detection body with the inner cavity 21 of the reaction container 20. In this embodiment, the first sub-connector 32 and the second sub-connector 33 are fixedly connected by gluing.

[0035] By providing the quick-release connector 30, the assembly speed of the light measuring device 100 can be improved, and the assembly can be performed quickly. Specifically, after the first sub-connector 32 and the second sub-connector 33 are fixedly connected, the first sub-connector 32 is connected to the detection body, and the second sub-connector 33 is connected to the reaction container 20. Thus, through the connection action of the quick-release connector 30, the light measuring window 11 of the detection body and the inner cavity 21 of the reaction container 20 can be connected. Therefore, by shortening the assembly time, the detection can be performed quickly, thereby improving the efficiency of the detection.

[0036] In this embodiment, the first sub-connector 32 has an insertion end 321 that can be plugged into the light-measuring window 11 of the detection body to securely fasten the first sub-connector 32 to the detection body. Specifically, a first positioning hole 322 is defined on the sidewall of the insertion end 321. This first positioning hole 322 is not a through hole. A second positioning hole 134 is defined on the bottom plate 13 corresponding to the first positioning hole 322. The second positioning hole 134 is located on the side surface 133 of the mounting end 131.

[0037] Further, please also refer to Figure 1-Figure 5The photometric device 100 further includes a guide member 50. When the insertion end 321 of the first sub-connector 32 is inserted into the photometric window 11 of the detection body, the guide member 50 extends through the second positioning hole 134 into the first positioning hole 322. The guide member 50 is tightened to secure the first sub-connector 32 to the detection body. In this embodiment, the guide member 50 is a screw. The guide member 50 not only improves the stability and reliability of the connection between the first sub-connector 32 and the detection body, but also limits the axial movement and circumferential rotation of the first sub-connector 32, preventing shaking of the first sub-connector 32 when connected to the detection body, which could affect measurement and improve measurement accuracy.

[0038] In this embodiment, the second sub-connector 33 has a first end surface 331, which faces away from the first sub-connector 32. A first protrusion 332 and a second protrusion 333 are spaced apart on the first end surface 331, surrounding the cavity 31. The first protrusion 332 and the second protrusion 333 cooperate to form a first groove 334, which is used to accommodate at least a portion of the reaction vessel 20. It is understood that the first protrusion 332 and the second protrusion 333 are both annular protrusions, and the first groove 334 is an annular groove. The bottom wall of the first groove 334 is formed by a portion of the first end surface 331. When the second sub-connector 33 is connected to the reaction vessel 20, the first groove 334 accommodates a portion of the reaction vessel 20, thereby reducing the height of the device 100 and thereby reducing the volume of the light measuring device 100.

[0039] Please also refer to Figure 6 In one embodiment, the injection needle 40 is made of steel and includes a first section 43 and a second section 44. The first section 43 extends through the side wall of the quick-release connector 30, and the second section 44 is bent and connected to the first section 43, with at least a portion of the second section 44 located within the inner cavity 21. In other embodiments, the second section 44 may be entirely located within the cavity 31 of the quick-release connector 30, as long as it can function to introduce the excitation solution into the inner cavity 21 of the reaction vessel 20.

[0040] The second section 44 is connected to the first section 43 by a bend, that is, the injection needle 40 is a curved needle. In this embodiment, the bend is an arc-shaped bend, that is, the connection between the second section 44 and the first section 43 is a circular arc transition. In other embodiments, the bend can also be a right-angled bend, a bend of other geometric shapes, or a combination of multiple geometric shapes, and the specific configuration can be determined according to actual conditions.

[0041] Further, please also refer to Figure 2 、 Figure 4 、 Figure 5 and Figure 7 Because the injection needle 40 is curved and difficult to assemble directly during assembly of the light measuring device 100, the first sub-connector 32 and the second sub-connector 33 cooperate to form a mounting hole 34 extending integrally through the side wall of the quick-release connector 30. The mounting hole 34 extends through the cavity 31. At least a portion of the first section 43 is located within the mounting hole 34, with both ends of the first section 43 extending outside the mounting hole 34. The mounting hole 34 provides space for the injection needle 40 to be installed on the quick-release connector 30 through the mounting hole 34, thereby enhancing the stability and reliability of the installation of the injection needle 40.

[0042] Specifically, the first sub-connector 32 has a first mounting hole 341 that passes through the side wall of the first sub-connector 32, and the second sub-connector 33 has a second mounting hole 342 that passes through the side wall of the second sub-connector 33. When the first sub-connector 32 and the second sub-connector 33 are mated and connected, the first mounting hole 341 and the second mounting hole 342 are directly connected to each other, thereby forming the mounting hole 34 that passes through the side wall of the quick-release connector 33 as a whole. Therefore, when the injection needle 40 is to be installed on the quick-release connector 30, the injection needle 40 can be first installed in the first mounting hole 341 of the first sub-connector 32, and then the second sub-connector 33 is fixedly connected to the first sub-connector 32, so that the second mounting hole 342 on the second sub-connector 33 cooperates with the first mounting hole 341 to jointly fix the injection needle 40. The setting of the first mounting hole 341 and the second mounting hole 342 allows the injection needle 40 to be quickly disassembled from the quick-release connector 30, thereby enhancing the convenience of assembly and avoiding deformation of the shape of the injection needle 40 due to unreasonable assembly methods during the assembly process.

[0043] Furthermore, the portion of the second section 44 extending into the inner cavity 21 is parallel to the central axis of the reaction vessel 20, so that the excitation liquid can be vertically injected into the luminous object in the inner cavity 21. In addition, the second section 44 is arranged close to the cavity wall of the inner cavity 21 to reduce the large space occupied by the injection needle 40 in the circumferential direction of the inner cavity 21, thereby avoiding the injection needle 40 from blocking the photomultiplier tube 10 from collecting photons. In other embodiments, the portion of the second section 44 extending into the inner cavity 21 may also form a certain angle with the central axis of the reaction vessel 20, that is, the excitation liquid is injected obliquely into the luminous object in the inner cavity 21.

[0044] For further information, see 2 and Figure 6 The two ends of the first section 43 are respectively located inside and outside the quick-release connector 30. The end of the first section 43 located outside the quick-release connector 30 is the second end 42 of the injection needle 40. The second end 42 is provided with a hollow adapter 45. The injection device (not shown) includes an injection tube and a liquid reservoir. The adapter 45 is used to connect to the injection tube. The injection tube is connected to the liquid reservoir that stores the excitation liquid. The excitation liquid in the liquid reservoir is injected into the inner cavity 21 of the reaction container 20 through the injection tube, the adapter 45, and the injection needle 40. The material used to make the injection tube should have good toughness, high fluidity, and chemical resistance. It should be noted that, because the reservoir is a certain distance from the reaction vessel 20, if the injection needle 40 were to extend directly from the reservoir and ultimately into the inner cavity 21 of the reaction vessel 20, the diameter of the injection needle 40 would be increased while ensuring strength. This is inconsistent with the trend of compactness and miniaturization of the light measuring device 100. Furthermore, since the injection needle 40 is made of steel, multiple bends to accommodate the dense component layout of the light measuring device 100 could easily cause the injection needle 40 to break. Therefore, an adapter 45 is provided to connect to the injection tube, forming an "injection needle 40 - adapter 45 - injection tube" arrangement. This makes the light measuring device 100 easy to assemble and disassemble and can accommodate various component layouts. It is understood that both the adapter 45 and the injection tube can be multiple, for example, forming an "injection needle 40 - adapter 45 - injection tube - adapter 45 - injection tube" arrangement.

[0045] Furthermore, the injection device also includes a power pump (not shown), which is connected to the injection tube. The power pump is used to make the excitation liquid flow, so as to guide the excitation liquid in the liquid storage tank to flow into the injection tube and finally be injected into the inner cavity 21 of the reaction container 20.

[0046] Please also refer to Figure 8 In this embodiment, the reaction vessel 20 should be made of an opaque material to prevent external light from affecting the detection accuracy of the photomultiplier tube 10. Furthermore, the material used to make the reaction vessel 20 should also possess good toughness, high fluidity, chemical resistance, grease resistance, alcohol resistance, and antibacterial properties. Preferably, the material used can be high-flow polycarbonate or high-flow acrylonitrile-styrene-butadiene copolymer.

[0047] The reaction vessel 20 has a second end surface 201. A third protrusion 202 and a fourth protrusion 203 are spaced apart on the second end surface 201 around the inner cavity 21. The third protrusion 202 and the fourth protrusion 203 cooperate to form a second groove 204. The second groove 204 is used to accommodate at least a portion of the second sub-connector 33. Specifically, the second end surface 201 has a sample injection port 205, which is connected to the inner cavity 21. The sample injection port 205 is the opening of the inner cavity 21 described above. By injecting an stimulating liquid into the sample injection port 205, the stimulating liquid and the luminescent material undergo a luminescent reaction in the inner cavity 21 of the reaction vessel 20. It will be understood that the third protrusion 202 and the fourth protrusion 203 are both annular protrusions, and the second groove 204 is an annular groove. The bottom wall of the second groove 204 is formed by a portion of the second end surface 201. When the second sub-connector 33 is connected to the reaction container 20 , the second groove 204 accommodates part of the second sub-connector 33 to reduce the device stacking in the height direction of the light measuring device 100 , thereby reducing the volume of the light measuring device 100 .

[0048] Please refer to Figure 9 and Figure 10 The light measuring device 100 also has a light shielding portion 60, which includes a fixing member 61 and a light shielding plate 62. The fixing member 61 is located on one side of the detection body. The light shielding plate 62 includes a first sub-light shielding plate 63 and a second sub-light shielding plate 64. The first sub-light shielding plate 63 is rotatably mounted on the bottom surface of the fixing member 61, and the second sub-light shielding plate 64 is bent and connected to the first sub-light shielding plate 63. The second light shielding plate 62 is located on the side of the quick-release joint 30 away from the detection body. The first sub-light shielding plate 63 is rotated by an external force, driving the second sub-light shielding plate 64 to cover or expose the quick-release joint 30.

[0049] Specifically, the fixing member 61 is located in the middle of one of the side surfaces of the housing 12, with the bottom surface being the surface of the fixing member 61 proximal to the light measuring window 11. The first sub-light shielding plate 63 includes a fixing section 631 and a connecting section 632. The fixing section 631 is screwed to the bottom surface 611 of the fixing member 61. The connecting section 632 is bent and connected to the fixing section 631. The end of the connecting section 632, distal from the fixing section 631, is bent and connected to the second sub-light shielding plate 64. The light measuring window 11 of the detection body is equipped with the quick-release connector 30. Since the bottom surface 611 of the fixing member 61 is still a certain distance away from the quick-release connector 30, the curved shape of the light shielding plate 62 facilitates full utilization of the space occupied by the light measuring device 100, improving space efficiency.

[0050] Further, please refer again to Figure 1 and Figure 2 , the detection body and the reaction container 20 are arranged in sequence along the first direction D1, and the central axis of the light measuring window 11 of the detection body and the central axis of the reaction container 20 are located on the same straight line. The detection body can be vertically moved along the first direction D1 by an external force to connect the quick-release connector 30 to the reaction container 20 or disconnect the reaction container 20. In this embodiment, the first direction D1 is the Z direction. When the detection body moves along the first direction D1 to disconnect the quick-release connector 30 from the reaction container 20, the first sub-light shielding plate 63 is rotated by the external force and drives the second sub-light shielding plate 64 to cover the quick-release connector 30, so as to prevent external light from entering the light measuring window 11 and affecting subsequent light measurement.

[0051] When the detection body moves along the first direction D1 to connect the quick-release connector 30 to the reaction container 20, in order to enable the light measuring window 11 to be aligned with the inner cavity 21 of the reaction container 20, the first sub-light shielding plate 63 is rotated by an external force to drive the second sub-light shielding plate 64 to expose the quick-release connector 30, so that the quick-release connector 30 can be smoothly connected to the reaction container 20.

[0052] For further information, please also refer to Figure 11 When the quick-release connector 30 is connected to the reaction vessel 20, the first protrusion 332 is inserted into the second groove 204, and the end of the first protrusion 332 facing away from the first end surface 331 abuts the second end surface 201. The fourth protrusion 203 is inserted into the first groove 334, and the end of the second protrusion 333 facing away from the second end surface 201 abuts the first end surface 331. As a result, along the central axis of the reaction vessel 20 and in directions perpendicular to opposite sides of the central axis, the third protrusion 202, the first protrusion 332, the fourth protrusion 203, and the second protrusion 333 are sequentially staggered to form a complex and tortuous light-shielding structure, thereby preventing light from outside the light measuring device 100 from entering the interior of the reaction vessel 20 and affecting the accuracy of light measurement. At this time, a gap exists between the peripheral side surface of the first protrusion 332 and the sidewall of the second groove 204, and a gap also exists between the peripheral side surface of the fourth protrusion 203 and the sidewall of the first groove 334. It is understandable that, due to the existence of the gap, the quick-release connector 30 is not engaged with the reaction container 20 , but the quick-release connector 30 only serves to seal the inner cavity 21 of the reaction container 20 .

[0053] The photometric device 100 described in the present application connects the photomultiplier tube 10 with the reaction container 20, and the photometric window 11 of the detection body can be connected to the inner cavity 21 of the reaction container 20 under the cooperation of the connecting part without setting up a reaction container transfer mechanism, thereby simplifying the structure of the photometric device 100, and by setting an injection needle 40 on the connecting part, and making the first end 41 of the injection needle 40 located in the cavity, and the second end 42 of the injection needle 40 located on the outside of the connecting part, so as to provide a channel for introducing the excitation liquid in the injection device into the inner cavity 21 of the reaction container 20, the excitation liquid causes the luminescent substance to emit photons under the excitation action of the excitation liquid, and then the photomultiplier tube 10 collects the number of photons generated in the reaction process in real time through the photometric window 11, and calculates the content of the luminescent substance based on the number of photons to complete the detection process. The detection process can realize photometry while adding the excitation liquid. On the one hand, it simplifies the detection process and improves the efficiency of detection. On the other hand, because the luminescence process is short, completing the photometry while injecting the excitation liquid can improve the accuracy of the detection and avoid the occurrence of large errors.

[0054] The embodiments of the present invention are described in detail above. Specific examples are used herein to illustrate the principles and implementation methods of the present invention. The description of the above embodiments is only used to help understand the method of the present invention and its core ideas. At the same time, for those skilled in the art, according to the ideas of the present invention, there may be changes in the specific implementation methods and application scopes. In summary, the contents of this specification should not be understood as limiting the present invention.

Claims

1. A light measuring device, characterized in that: The photometric device comprises a photomultiplier tube, a reaction container and an injection needle. The photomultiplier tube comprises a detection body and a connection part. The detection body has a photometric window. The connection part has a cavity with two ends opened, and the cavity is connected to the photometric window. The connection part is used to be detachably connected to the reaction container. The reaction container has an inner cavity opened at one end, and the inner cavity is used to accommodate a luminescent object to be detected. When the photomultiplier tube is connected to the reaction container, the cavity is connected to the photometric window and the inner cavity. The injection needle is arranged on the connection part. The first end of the injection needle is located in the cavity and the projection of the first end in the direction of the inner cavity is located in the inner cavity. The second end of the injection needle is located outside the connection part, and is used for introducing the excitation liquid provided by the injection device into the inner cavity of the reaction container to react with the luminescent object accommodated in the inner cavity to make the luminescent object emit photons. The photomultiplier tube is used to collect the photons through the photometric window at the same time to detect the content of the luminescent object. Among them, the connecting part is a quick-release joint, and the quick-release joint includes a first sub-joint and a second sub-joint connected to each other, the end of the first sub-joint facing away from the second sub-joint is connected to the detection body, and the end of the second sub-joint facing away from the first sub-joint is connected to the reaction container, and the detection body and the reaction container are detachably connected through the quick-release joint, and the first sub-joint and the second sub-joint cooperate to form an integral mounting hole that penetrates the side wall of the quick-release joint, and the injection needle is penetrated through the mounting hole and is arranged on the side wall of the quick-release joint.

2. The light measuring device according to claim 1, characterized in that The injection needle includes a first section and a second section, the first section passes through the side wall of the quick-release connector, the second section is bent and connected to the first section, and at least a portion of the second section is located in the inner cavity.

3. The light measuring device according to claim 2, characterized in that The two ends of the first section are respectively located on the inner and outer sides of the quick-release joint, the end of the first section located on the outer side of the quick-release joint is the second end of the injection needle, and the second end is provided with a hollow adapter. The injection device includes an injection tube and a liquid storage tank. The adapter is used to be connected to the injection tube, and the injection tube is connected to the liquid storage tank for storing the excitation liquid. The excitation liquid in the liquid storage tank is injected into the inner cavity of the reaction container through the injection tube, the adapter and the injection needle.

4. The light measuring device according to claim 3, characterized in that The first sub-connector and the second sub-connector cooperate to seal the photometric device to form a closed cavity including the cavity and an inner cavity connected to the cavity, and align the photometric window of the detection body with the inner cavity of the reaction container.

5. The light measuring device according to claim 4, characterized in that The mounting hole is connected to the cavity, at least a portion of the first section is located in the mounting hole, and two ends of the first section extend outside the mounting hole respectively.

6. The light measuring device according to claim 5, characterized in that The first sub-connector has a first mounting hole that passes through the side wall of the first sub-connector, and the second sub-connector has a second mounting hole that passes through the side wall of the second sub-connector. When the first sub-connector is connected with the second sub-connector, the first mounting hole is connected to the second mounting hole to form the mounting hole that passes through the side wall of the quick-release connector as a whole.

7. The light measuring device according to claim 5, characterized in that The second sub-connector has a first end face, which is the end face of the second sub-connector facing away from the first sub-connector, and a first protrusion and a second protrusion are arranged on the first end face around the cavity, and the first protrusion and the second protrusion cooperate to form a first groove, and the first groove is used to accommodate at least part of the reaction container.

8. The light measuring device according to claim 7, characterized in that The reaction container has a second end face, on which a third protrusion and a fourth protrusion are arranged around the inner cavity, and the third protrusion and the fourth protrusion cooperate to form a second groove, and the second groove is used to accommodate at least a portion of the second sub-connector.

9. The light measuring device according to claim 8, characterized in that When the first protrusion is inserted into the second groove and the end of the first protrusion facing away from the first end face abuts against the second end face, and the fourth protrusion is inserted into the first groove and the end of the second protrusion facing away from the second end face abuts against the first end face, there is a gap between the peripheral side surface of the first protrusion and the groove side wall of the second groove, and there is also a gap between the peripheral side surface of the fourth protrusion and the groove side wall of the first groove.

10. The light measuring device according to claim 1, wherein: The light measuring device also has a shading part, which includes a fixing part and a shading plate. The fixing part is located on a side surface of the detection body. The shading plate includes a first sub-light shading plate and a second sub-light shading plate. The first sub-light shading plate is rotatably mounted on the bottom surface of the fixing part. The second sub-light shading plate is bent and connected to the first sub-light shading plate and the second sub-light shading plate is located on the side of the quick-release joint away from the detection body. The first sub-light shading plate is rotated by an external force to drive the second sub-light shading plate to cover or expose the quick-release joint.

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