Isolated lung storage and perfusion device

Abstract

The present invention relates to an isolated lung storage and perfusion device, which comprises a storage and perfusion box; a perfusion circulation mechanism and a breathing circulation mechanism are arranged in the storage and perfusion box, and the perfusion circulation mechanism and the breathing circulation mechanism are both electrically connected to a perfusion breathing controller in the storage and perfusion box; after the perfusion circulation mechanism is connected to the pulmonary artery and pulmonary vein of the isolated lung through an adapter, the perfusion breathing controller controls the perfusion circulation mechanism to continuously perfuse the connected isolated lung with perfusion liquid, and can remove the metabolic products generated by the isolated lung during the perfusion liquid perfusion process; the perfusion breathing controller controls the breathing circulation mechanism to assist the isolated lung in breathing, so that the isolated lung can maintain a normal breathing state. The present invention can effectively realize the cleaning and perfusion of the isolated lung during the transportation process, effectively remove the metabolic products generated during the transportation process of the isolated lung, provide the respiratory circulation of the isolated lung during the transportation process, and ensure the function of the isolated lung after transportation, which is safe and reliable.

Description

Technical Field

[0001] The invention relates to a storage and perfusion device, in particular to an isolated lung storage and perfusion device, belonging to the technical field of isolated lung storage. Background Art

[0002] During lung transplant surgery, the ex vivo lungs need to be removed first. After being removed, the ex vivo lungs are perfused with perfusion fluid and placed in an ex vivo lung transport box, and then the ex vivo lung transport box and the ex vivo lungs are transported to the destination. Generally, the transport time of the ex vivo lungs can be up to 6 to 7 hours or even longer, but during the transport of the ex vivo lungs, since perfusion operations cannot be performed in the transport box, the metabolic products of the ex vivo lungs cannot be discharged from the ex vivo lungs. After the ex vivo lungs are transplanted, the metabolic products in the ex vivo lungs follow the body's circulation into the recipient's blood, causing even more severe reactions in the recipients of the ex vivo lungs.

[0003] In addition, the ex vivo lungs are in a stable state in the low-temperature environment of the transport box, which makes it impossible for the ex vivo lungs to maintain a normal breathing state. It will also cause greater damage to the ex vivo lungs, reduce the function of the ex vivo lungs, and in severe cases lead to the failure of the lung transplantation surgery. Summary of the invention

[0004] The purpose of the present invention is to overcome the deficiencies in the prior art and provide an isolated lung storage and perfusion device, which can effectively realize the cleaning and perfusion of the isolated lung during the transport process, effectively remove the metabolic products generated during the transport of the isolated lung, provide the respiratory circulation of the isolated lung during the transport process, ensure the function of the isolated lung after transport, and be safe and reliable.

[0005] According to the technical solution provided by the present invention, the isolated lung storage and perfusion device comprises a storage and perfusion box body that can be used for storing and transporting the isolated lung; a perfusion circulation mechanism that can be adaptively connected to the isolated lung and a breathing circulation mechanism that can be adaptively connected to the isolated lung are arranged in the storage and perfusion box body, and the perfusion circulation mechanism and the breathing circulation mechanism are both electrically connected to the perfusion and breathing controller in the storage and perfusion box body, and the corresponding working states of the perfusion circulation mechanism and the breathing circulation mechanism can be controlled by the perfusion and breathing controller;

[0006] After the perfusion circulation mechanism is connected to the pulmonary artery and pulmonary vein of the isolated lung, the perfusion breathing controller controls the perfusion circulation mechanism to continuously perfuse the connected isolated lung with perfusion fluid, and can remove metabolic products produced by the isolated lung during the perfusion fluid perfusion process; the perfusion breathing controller controls the breathing circulation mechanism to assist the isolated lung in breathing, so that the isolated lung can maintain a normal breathing state.

[0007] A heat-insulating layer is arranged in the storage and perfusion box, and a storage and transportation refrigeration source capable of providing a low-temperature environment required for storage and transportation of ex vivo lungs is also arranged in the storage and perfusion box.

[0008] The storage and transportation refrigeration source includes ice cubes placed in the storage and perfusion box or a semiconductor refrigerator that can achieve working refrigeration. The semiconductor refrigerator is connected to a perfusion breathing controller that controls the working state of the semiconductor refrigerator. The perfusion breathing controller can collect the temperature inside the storage and perfusion box in real time through a temperature sensor. The perfusion breathing controller can make the storage and perfusion box reach the temperature environment for ex vivo lung transportation through the temperature sensor and the semiconductor refrigerator.

[0009] The perfusion circulation mechanism includes a perfusion fluid device capable of containing perfusion fluid, a perfusion driver adapted to the perfusion fluid device, and a first pulmonary vein connecting tube, a second pulmonary vein connecting tube, a first pulmonary artery connecting tube, and a second pulmonary artery connecting tube capable of communicating with the perfusion fluid device;

[0010] The first pulmonary vein connecting tube and the second pulmonary vein connecting tube can be connected to the pulmonary vein adapter, the first pulmonary artery connecting tube and the second pulmonary artery connecting tube can be connected to the pulmonary artery adapter, the perfusion fluid in the perfusion fluid device can be driven by the perfusion driver to enter the pulmonary artery of the isolated lung via the first pulmonary artery connecting tube and the second pulmonary artery connecting tube, and the perfusion fluid entering the isolated lung can be returned to the perfusion fluid device via the first pulmonary vein connecting tube and the second pulmonary vein connecting tube, so as to realize a continuous perfusion fluid perfusion cycle of the isolated lung.

[0011] It also includes a perfusion liquid outlet tube and a perfusion liquid inlet tube that can be adapted to be connected to the perfusion liquid device, one end of the perfusion liquid inlet tube is connected to the perfusion liquid device, the other end of the perfusion liquid inlet tube is connected to and communicated with the first pulmonary vein connecting tube and the second pulmonary vein connecting tube, one end of the perfusion liquid outlet tube is connected to the perfusion liquid device, and the other end of the perfusion liquid outlet tube is connected to and communicated with the first pulmonary artery connecting tube and the second pulmonary artery connecting tube;

[0012] A filter remover for removing metabolites in the isolated lung is arranged on the perfusion outlet pipe, and a gas separator for discharging gas in the perfusion liquid is arranged on the perfusion inlet pipe.

[0013] A liquid outlet one-way valve is arranged in the end of the perfusion liquid outlet tube adjacent to the perfusion liquid device, and a liquid inlet one-way valve is arranged in the end of the perfusion liquid inlet tube adjacent to the perfusion liquid device;

[0014] The perfusion liquid device comprises a perfusion liquid container capable of containing perfusion liquid and a perfusion driving elastic ring arranged at the upper inner part of the perfusion liquid container. The perfusion driving elastic ring is adaptably connected to the perfusion liquid container. The perfusion driving elastic ring can be driven to reciprocate in the perfusion liquid container by a perfusion driver. When the perfusion driving elastic ring reciprocates in the perfusion liquid container, the perfusion liquid in the perfusion liquid inlet pipe can enter the perfusion liquid receiving chamber at the lower part of the perfusion liquid container, and the perfusion liquid in the perfusion liquid receiving chamber can enter the perfusion liquid outlet pipe.

[0015] The gas separator comprises a plurality of exhaust holes arranged on the perfusion inlet pipe and a water-blocking and breathable membrane arranged in the exhaust holes, and the filter remover comprises an adsorption filter membrane body capable of adsorbing and separating the metabolites.

[0016] A first connecting tube clamp is arranged on the first pulmonary vein connecting tube, a second connecting tube clamp is arranged on the second pulmonary vein connecting tube, a third connecting tube clamp is arranged on the first pulmonary artery connecting tube, and a fourth connecting tube clamp is arranged on the second pulmonary artery connecting tube.

[0017] The breathing circulation mechanism includes a gas generator capable of extracting gas stored in a perfusion box, an extraction driver capable of driving the gas generator, and a gas filter capable of filtering the gas extracted by the gas generator. The gas outlet of the gas filter is provided with a tracheal tube capable of matching with the trachea of ​​an isolated lung. The extraction driver is electrically connected to a perfusion breathing controller, and the perfusion breathing controller can control the extraction driver to drive the gas generator to enter a desired working state.

[0018] The gas generator includes a generator shell, a gas extraction elastic ring arranged in the generator shell, and a filter air inlet arranged at the bottom of the generator shell; the extraction driver can drive the gas extraction elastic ring to reciprocate in the generator shell. When the gas extraction elastic ring reciprocates in the generator shell, the gas stored in the perfusion box can enter the generator shell through the filter air inlet, and the gas in the generator shell can enter the trachea of ​​the isolated lung through the gas filter and the tracheal tube.

[0019] The advantages of the present invention are as follows: the storage and transportation of the ex vivo lungs can be realized through the storage and perfusion box, the continuous perfusion of the ex vivo lungs during the storage and transportation process can be realized through the perfusion circulation mechanism in the storage and perfusion box, the respiratory support of the ex vivo lungs during the storage and transportation process can be realized through the respiratory circulation mechanism, the perfusion and cleaning of the ex vivo lungs during the transportation process can be effectively realized, the metabolic products generated during the transportation process of the ex vivo lungs can be effectively removed, the respiratory circulation of the ex vivo lungs during the transportation process can be provided, and the function of the ex vivo lungs after transportation can be ensured, which is safe and reliable. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a structural schematic diagram of the present invention.

[0021] Figure 2 It is a schematic structural diagram of the first box cover of the present invention.

[0022] Figure 3 It is a schematic structural diagram of the second box cover of the present invention.

[0023] Figure 4 It is a schematic diagram of the perfusion circulation mechanism of the present invention.

[0024] Figure 5It is a schematic diagram of a specific implementation of the perfusion circulation mechanism of the present invention.

[0025] Figure 6 It is a schematic diagram of the breathing circulation mechanism of the present invention.

[0026] Explanation of the reference numerals: 1-storage perfusion box, 2-insulation layer, 3-box pull ring, 4-box power supply, 5-perfusion circulation mechanism, 6-breathing circulation mechanism, 7-first box cover, 8-perfusion circulation placement area, 9-perfusion elastic ring driver, 10-perfusion elastic ring drive plate, 11-second box cover, 12-breathing circulation placement area, 13-extraction elastic ring driver, 14-extraction elastic ring drive plate, 15-first pulmonary vein connecting pipe, 16-second pulmonary vein connecting pipe, 17-first connecting pipe clamp, 18-second connecting pipe clamp, 19-perfusion liquid inlet pipe, 20-gas separation container, 21-water-blocking breathable membrane , 22-liquid inlet one-way valve, 23-infusion liquid device, 24-liquid outlet one-way valve, 25-filter remover, 26-infusion liquid outlet pipe, 27-third connecting pipe clamp, 28-fourth connecting pipe clamp, 29-first pulmonary artery connecting pipe, 30-second pulmonary artery connecting pipe, 31-infusion port, 32-infusion liquid receiving container, 33-infusion drive elastic ring, 34-infusion liquid receiving chamber, 35-filter removal container, 36-adsorption filter membrane body, 37-generator shell, 38-gas extraction elastic ring, 39-filter air inlet, 40-gas filter, 41-tracheal tube, 42-tracheal insertion end and 43-exhaust valve. DETAILED DESCRIPTION

[0027] The present invention will be further described below in conjunction with specific drawings and embodiments.

[0028] like Figure 1 , Figure 2 and Figure 3 As shown: In order to achieve perfusion and cleaning of the ex vivo lung during transportation, effectively remove the metabolic products generated during the ex vivo lung transportation process, provide the ex vivo lung with respiratory circulation during transportation, and ensure the function of the ex vivo lung after transportation, the present invention includes a storage and perfusion box 1 that can be used for storage and transportation of the ex vivo lung; a perfusion circulation mechanism 5 that can be adaptively connected to the ex vivo lung and a respiratory circulation mechanism 6 that can be adaptively connected to the ex vivo lung are arranged in the storage and perfusion box 1, and the perfusion circulation mechanism 5 and the respiratory circulation mechanism 6 are both electrically connected to the perfusion and breathing controller in the storage and perfusion box 1, and the corresponding working states of the perfusion circulation mechanism 5 and the respiratory circulation mechanism 6 can be controlled by the perfusion and breathing controller;

[0029] After the perfusion circulation mechanism 5 is connected to the pulmonary artery and pulmonary vein of the isolated lung, the perfusion breathing controller controls the perfusion circulation mechanism 5 to continuously perfuse the connected isolated lung with perfusion fluid, and can remove the metabolic products produced by the isolated lung during the perfusion fluid perfusion process; the perfusion breathing controller controls the breathing circulation mechanism 6 to assist the isolated lung in breathing, so that the isolated lung can maintain a normal breathing state.

[0030] Specifically, the storage and perfusion box 1 is made of materials that meet medical standards. The specific material type can be selected as needed, which is well known to those skilled in the art and will not be described in detail here. The storage and perfusion box 1 can be used to store and reuse isolated lungs. During specific implementation, a perfusion circulation mechanism 5, a respiratory circulation mechanism 6, and a perfusion breathing controller are arranged in the storage and perfusion box 1. The perfusion circulation mechanism 5 and the respiratory circulation mechanism 6 can be connected and matched with the isolated lung in the storage and perfusion box 1. The perfusion breathing controller can be electrically connected to the perfusion circulation mechanism 5 and the respiratory circulation mechanism 6, that is, the perfusion breathing controller can control the corresponding working states of the perfusion circulation mechanism 5 and the respiratory circulation mechanism 6.

[0031] In specific implementation, the perfusion circulation mechanism 5 can be connected with the pulmonary artery and pulmonary vein of the isolated lung, and the connected isolated lung can be continuously perfused with perfusion fluid through the perfusion circulation mechanism 5. During the perfusion process, the metabolic products produced by the isolated lung can be removed, and at the same time, the gas in the perfusion fluid can be separated and discharged, thereby effectively reducing the reaction of the isolated lung after transplantation and ensuring the reliability of the isolated lung after transplantation. The breathing circulation mechanism 6 can assist the isolated lung in breathing, maintain the breathing state of the isolated lung during transportation, and ensure the function of the isolated lung during transportation.

[0032] Specifically, the perfusion breathing controller can adopt the existing commonly used microprocessor form, such as a single-chip microcomputer, etc., which can be selected according to needs and will not be repeated here. Generally, in order to adapt to the storage of ex vivo lungs, the storage perfusion box 1 includes a first box cover 7 and a second box cover 11 adapted to the first box cover 7. When the first box cover 7 and the second box cover 11 are close to each other, a storage perfusion box 1 can be formed, so that the ex vivo lung can be stored in a closed manner; when the first box cover 7 and the second box cover 11 are separated from each other, the ex vivo lung can be placed in the storage perfusion box 1. A box pull ring 3 is provided on the first box cover 7 and / or the second box cover 11, and the storage perfusion box 1 can be conveniently held by the box pull ring 3.

[0033] In the embodiment of the present invention, the perfusion circulation mechanism 5 is arranged in the perfusion circulation placement area 8 in the first box cover 7, and the breathing circulation mechanism 6 is arranged in the breathing circulation placement area 12 in the second box cover 11. A cavity adapted for the isolated lung can be formed between the first box cover 7 and the second box cover 11, so that when the isolated lung is placed between the first box cover 7 and the second box cover 11, the perfusion circulation mechanism 5 and the breathing circulation mechanism 6 can be adapted and connected with the isolated lung to achieve continuous perfusion and auxiliary breathing support for the isolated lung. A box power supply 4 required for the operation of the perfusion breathing controller needs to be provided in the storage perfusion box 1, and the box power supply 4 can adopt a lithium battery or other power supply form, and the box power supply 4 can adopt a rechargeable or replaceable form. A commonly used connection form can be adopted between the box power supply 4 and the perfusion breathing controller, which is specifically well known to those skilled in the art and will not be repeated here.

[0034] Furthermore, a thermal insulation layer 2 is provided in the storage perfusion box 1, and a storage and transportation refrigeration source capable of providing a low-temperature environment required for storage and transportation of ex vivo lungs is also provided in the storage and perfusion box 1. In the embodiment of the present invention, the storage and transportation refrigeration source can provide the temperature conditions required for the ex vivo lungs in the storage and perfusion box 1, thereby ensuring the safety and reliability of the ex vivo lungs in the storage and perfusion box 1. The thermal insulation layer 2 can reduce the heat exchange of the storage and transportation refrigeration source with the outside world, thereby ensuring the temperature stability in the storage and perfusion box 1. The thermal insulation layer 2 is inside the first box cover 7 and the second box cover 11. The thermal insulation layer 2 can adopt an existing commonly used form, as long as it can achieve the purpose of thermal insulation, and the specific situation will not be repeated.

[0035] The storage and transportation refrigeration source includes ice cubes placed in the storage and perfusion box 1 or a semiconductor refrigerator that can achieve working refrigeration. The semiconductor refrigerator is connected to a perfusion breathing controller that controls the working state of the semiconductor refrigerator. The perfusion breathing controller can collect the temperature in the storage and perfusion box 1 in real time through a temperature sensor. The perfusion breathing controller can make the storage and perfusion box 1 reach the temperature environment for ex vivo lung transportation through the temperature sensor and the semiconductor refrigerator.

[0036] In the embodiment of the present invention, when ice cubes are arranged in the storage and perfusion box 1, the low temperature of the ice cubes can make the temperature conditions in the storage and perfusion box 1 meet the temperature conditions required for the storage and transportation of the ex vivo lungs. The form of ice cube refrigeration is consistent with the refrigeration method used in the existing storage and transportation of ex vivo lungs. Of course, in the specific implementation, a semiconductor refrigerator can also be used. The semiconductor refrigerator can adopt the existing commonly used form. The semiconductor refrigerator is electrically connected to the perfusion breathing controller. When the semiconductor refrigerator is working, the temperature condition in the storage and perfusion box 1 can be maintained at -5°C to 10°C. Of course, other refrigeration forms can also be used in the storage and perfusion box 1, as long as they can meet the temperature environment of the ex vivo lungs during the storage and transportation process in the storage and perfusion box 1, and they will not be listed here one by one.

[0037] like Figure 4 and Figure 5 As shown, the perfusion circulation mechanism 5 includes a perfusion fluid device 23 capable of containing perfusion fluid, a perfusion driver adapted to the perfusion fluid device 23, and a first pulmonary vein connecting tube 15, a second pulmonary vein connecting tube 16, a first pulmonary artery connecting tube 29, and a second pulmonary artery connecting tube 30 capable of communicating with the perfusion fluid device 23;

[0038] The first pulmonary vein connecting tube 15 and the second pulmonary vein connecting tube 16 can be adapted to be connected to the pulmonary vein, and the first pulmonary artery connecting tube 29 and the second pulmonary artery connecting tube 30 can be adapted to be connected to the pulmonary artery. The perfusion driver can drive the perfusion fluid in the perfusion fluid device 23 to enter the pulmonary artery of the isolated lung via the first pulmonary artery connecting tube 29 and the second pulmonary artery connecting tube 30, and the perfusion fluid entering the isolated lung can return to the perfusion fluid device 23 again via the first pulmonary vein connecting tube 15 and the second pulmonary vein connecting tube 16, so as to realize a continuous perfusion fluid perfusion cycle of the isolated lung.

[0039] In the embodiment of the present invention, the perfusion liquid can be accommodated by the perfusion liquid device 23. The perfusion liquid can be placed in the perfusion liquid device 23 by pre-filling or perfusion. The perfusion liquid is an existing liquid that can be used to perfuse the isolated lung. The specific form of the perfusion liquid is well known to those skilled in the art and will not be described in detail here. The perfusion driver is adapted to the perfusion liquid device 23. The perfusion driver can provide power for the circulation of the perfusion liquid in the perfusion liquid device 23 to achieve the perfusion circulation of the perfusion liquid. In order to cooperate with the isolated lung, the first pulmonary vein connecting tube 15 and the second pulmonary vein connecting tube 16 can be connected to the pulmonary vein of the isolated lung, and the first pulmonary artery connecting tube 29 and the second pulmonary artery connecting tube 30 can be adapted to connect to the pulmonary artery of the isolated lung. After being connected to the isolated lung adapter and the perfusion fluid device 23 is driven by the perfusion driver, the perfusion fluid in the perfusion fluid device 23 can enter the pulmonary artery of the isolated lung via the first pulmonary artery connecting tube 29 and the second pulmonary artery connecting tube 30, and the perfusion fluid entering the isolated lung can return to the perfusion fluid device 23 again via the first pulmonary vein connecting tube 15 and the second pulmonary vein connecting tube 16. As the perfusion driver and the perfusion fluid device 23 continue to cooperate, the circulation movement of the perfusion fluid can be continuously realized, thereby realizing continuous perfusion circulation of the perfusion fluid to the isolated lung.

[0040] Furthermore, it also includes a perfusion liquid outlet tube 26 and a perfusion liquid inlet tube 19 that can be adapted to be connected to the perfusion liquid device 23, one end of the perfusion liquid inlet tube 19 is connected to the perfusion liquid device 23, the other end of the perfusion liquid inlet tube 19 is connected and communicated with the first pulmonary vein connecting tube 15 and the second pulmonary vein connecting tube 16, one end of the perfusion liquid outlet tube 26 is connected to the perfusion liquid device 23, and the other end of the perfusion liquid outlet tube 26 is connected and communicated with the first pulmonary artery connecting tube 29 and the second pulmonary artery connecting tube 30;

[0041] A filter remover 25 for removing metabolites in the isolated lung is provided on the perfusion outlet pipe 26, and a gas separator for discharging gas in the perfusion liquid is provided on the perfusion inlet pipe 19.

[0042] In the embodiment of the present invention, the diameter of the perfusion inlet pipe 19 is larger than the diameter of the first pulmonary vein connecting pipe 15 and the second pulmonary vein connecting pipe 16, and the length direction of the first pulmonary vein connecting pipe 15 and the second pulmonary vein connecting pipe 16 is consistent with the length direction of the perfusion inlet pipe 19, and the first pulmonary vein connecting pipe 15 and the second pulmonary vein connecting pipe 16 can be connected to the perfusion liquid device 23 through the perfusion inlet pipe 19. Similarly, the diameter of the perfusion outlet pipe 26 is larger than the diameter of the first pulmonary artery connecting pipe 29 and the diameter of the second pulmonary artery connecting pipe 30, and the diameter of the first pulmonary artery connecting pipe 29 and the second pulmonary artery connecting pipe 30 can be connected to the perfusion liquid device 23 through the perfusion outlet pipe 26.

[0043] A filter remover 25 is provided on the perfusion liquid outlet pipe 26, and a gas separator is provided on the perfusion liquid inlet pipe 19. The gas separator comprises a plurality of exhaust holes provided on the perfusion liquid inlet pipe 19 and a water-blocking and breathable membrane 21 provided in the exhaust holes, and the filter remover 25 comprises an adsorption filter membrane body 36 capable of adsorbing and separating the metabolites. Specifically, the gas separator includes a gas separation container 20 that can be connected to the perfusion inlet pipe 19. A plurality of exhaust holes are arranged on the gas separation container 20. A water-blocking breathable membrane 21 is arranged in each exhaust hole. The perfusion liquid that enters the perfusion liquid device 23 through the perfusion inlet pipe 19 first enters the gas separation container 20. When there is gas in the perfusion liquid, the gas in the perfusion liquid can be discharged through the water-blocking breathable membrane 21. The water-blocking breathable membrane 21 can prevent the perfusion liquid from being discharged from the exhaust hole along with the gas. After the gas is discharged, the perfusion liquid enters the perfusion liquid device 23 again. As the perfusion continues, the gas can be effectively discharged to avoid the problem of air embolism caused by the presence of gas in the perfusion liquid. Generally, a liquid injection port 31 can also be provided at the bottom of the gas separation container 20, through which the required liquid medicine, such as physiological saline, perfusion liquid, etc., can be injected into the gas separation container 20. The injected liquid can be mixed with the perfusion liquid in the gas separation container 20 and follow the perfusion cycle of the perfusion liquid.

[0044] In the embodiment of the present invention, the filter removal device 25 also includes a filter removal container 35, and the adsorption filter membrane body 36 is arranged in the filter removal container 35. The perfusion liquid entering the pulmonary artery from the perfusion liquid device 23 will first enter the filter removal container 35. After the perfusion liquid is fully in contact with the adsorption filter membrane body 36 in the filter removal container 35, it enters the pulmonary artery through the first pulmonary artery connecting tube 29 and the second pulmonary artery connecting tube 30. The metabolic products produced by the isolated lung can be adsorbed and removed by the adsorption filter membrane body 36. The adsorption filter membrane body 36 can be placed horizontally and vertically in the filter removal container 35. At this time, the perfusion liquid in the perfusion liquid device 23 first enters the lower part of the adsorption filter membrane body 36, and the perfusion liquid flows out of the filter removal container 35 through the upper part of the filter removal container 35 after passing through the adsorption filter membrane body 36; of course, the adsorption filter membrane body 36 can also adopt other distribution forms, as long as the perfusion liquid can be fully in contact. The adsorption filtration membrane body 36 can adopt the combination of the existing commonly used filter membrane and the medical adsorbent. The medical adsorbent can be medical carbon powder, etc. The adsorption filtration membrane body 36 can also adopt other structural forms, as long as it can achieve the adsorption and filtration of the metabolic products generated during the ex vivo lung perfusion process. The details will not be described in detail.

[0045] Furthermore, a liquid outlet check valve 24 is provided in the end of the perfusion liquid outlet pipe 26 adjacent to the perfusion liquid device 23, and a liquid inlet check valve 22 is provided in the end of the perfusion liquid inlet pipe 19 adjacent to the perfusion liquid device 23;

[0046] The perfusion liquid device 23 includes a perfusion liquid container 32 that can accommodate perfusion liquid and a perfusion driving elastic ring 33 arranged at the upper part of the perfusion liquid container 32. The perfusion driving elastic ring 33 is adaptably connected to the perfusion liquid container 32. The perfusion driving elastic ring 33 can be driven to reciprocate in the perfusion liquid container 32 by the perfusion driver. When the perfusion driving elastic ring 33 reciprocates in the perfusion liquid container 32, the perfusion liquid in the perfusion liquid inlet pipe 19 can enter the perfusion liquid receiving chamber 34 at the lower part of the perfusion liquid container 32, and the perfusion liquid in the perfusion liquid receiving chamber 34 can enter the perfusion liquid outlet pipe 26.

[0047] In the embodiment of the present invention, the liquid outlet one-way valve 24 and the liquid inlet one-way valve 22 can adopt existing commonly used forms. The one-way flow of the perfusion liquid can be achieved through the liquid inlet one-way valve 22 and the liquid outlet one-way valve 24. The liquid inlet one-way valve 22 is located between the gas separation container 20 and the perfusion liquid device 23, and the liquid outlet one-way valve 24 is located between the perfusion liquid device 23 and the filter removal container 35. The reliability of the flow of the perfusion liquid during the perfusion process can be ensured by the liquid inlet one-way valve 22 and the liquid outlet one-way valve 24.

[0048] The perfusion liquid device 23 can contain perfusion liquid through the perfusion liquid receiving container 32. The perfusion driving elastic ring 33 is located at the upper part of the perfusion liquid receiving container 32. The perfusion driving elastic ring 33 can be made of elastic components such as springs. The length direction of the perfusion driving elastic ring 33 is consistent with the length direction of the perfusion liquid receiving container 32. The upper part of the perfusion liquid receiving container 32 can reciprocate with the perfusion driving elastic ring 33, that is, the upper part of the perfusion liquid receiving container 32 can be made of an elastic film, so that the perfusion driving elastic ring 33 can be contained in the perfusion liquid receiving container 32, and the reciprocating motion of the perfusion driving elastic ring 33 in the perfusion liquid receiving container 32 can be ensured. The reciprocating direction of the perfusion driving elastic ring 33 is the length direction of the perfusion driving elastic ring 33. When the perfusion driving elastic ring 33 is compressed, negative pressure can be generated in the perfusion liquid receiving container 32, so that the perfusion liquid in the liquid inlet pipe 19 can enter the perfusion liquid receiving chamber 34 at the lower part of the perfusion liquid receiving container 32 through the liquid inlet one-way valve 22. When the perfusion driving elastic ring 33 is reset, the perfusion liquid in the perfusion liquid receiving chamber 34 can enter the perfusion liquid outlet pipe 26 through the liquid outlet one-way valve 24.

[0049] When the perfusion liquid device 23 adopts the above structure, the perfusion driver can drive the perfusion drive elastic ring 33 to reciprocate; in specific implementation, the perfusion driver includes a perfusion elastic ring driver body 9 and a perfusion elastic ring driver plate 10, the perfusion elastic ring driver body 9 is installed on the first box cover 7, the perfusion elastic ring driver plate 10 is located directly above the perfusion drive elastic ring 33, the perfusion elastic driver body 9 can adopt a linear motor, the perfusion elastic ring driver plate 10 is adaptively connected to the perfusion elastic driver body 9, and the perfusion elastic driver body 9 can drive the perfusion drive elastic ring 33 to reciprocate through the perfusion elastic ring driver plate 10. Of course, the perfusion elastic driver body 9 can also adopt the form of cooperation between a motor and a screw nut, etc., as long as the perfusion drive plate 10 can realize the reciprocating motion of the perfusion drive elastic ring 33, and the specific selection can be made according to needs, which will not be repeated here. The perfusion elastic driving body 9 is electrically connected to the perfusion breathing controller, and the perfusion breathing controller can control the working state of the perfusion elastic driving body 9, and further can realize the driving control of the reciprocating motion of the perfusion driving elastic ring 33.

[0050] Furthermore, a first connecting tube clamp 17 is provided on the first pulmonary vein connecting tube 15 , a second connecting tube clamp 18 is provided on the second pulmonary vein connecting tube 16 , a third connecting tube clamp 27 is provided on the first pulmonary artery connecting tube 29 , and a fourth connecting tube clamp 28 is provided on the second pulmonary artery connecting tube 30 .

[0051] In the embodiment of the present invention, the state of the perfusion fluid flowing through the first pulmonary vein connecting tube 15 can be controlled by the first connecting tube clamp 17. The first connecting tube clamp 17 can adopt an existing commonly used medical clamp, which can be selected according to needs and will not be described in detail here. Similarly, the specific functions of the second connecting tube clamp 18, the third connecting tube clamp 27 and the fourth connecting tube clamp 28 are consistent with those of the first connecting tube clamp 17, and the description of the first connecting tube clamp 17 can be referred to for details.

[0052] Furthermore, the breathing circulation mechanism 6 includes a gas generator that can extract the gas stored in the perfusion box 1, an extraction driver that can drive the gas generator, and a gas filter 40 that can filter the gas extracted by the gas generator. The gas outlet of the gas filter 40 is provided with a tracheal tube 41 that can adapt to the trachea of ​​the isolated lung. The extraction driver is electrically connected to the perfusion breathing controller, and the perfusion breathing controller can control the extraction driver to drive the gas generator to enter the desired working state.

[0053] In the embodiment of the present invention, the gas in the storage and perfusion box 1 can be extracted by the gas generator, the gas generator can be driven by the extraction driver, and the gas to be introduced into the trachea of ​​the isolated lung can be filtered by the gas filter 40 to prevent impurities in the gas from entering the trachea of ​​the isolated lung. The tracheal tube 41 is adapted to the isolated lung, and the tracheal tube 41 has a tracheal insertion end 42, that is, the tracheal tube 41 can be inserted into the trachea of ​​the isolated lung through the tracheal insertion end 42. The extraction driver is electrically connected to the perfusion breathing controller. When the perfusion breathing controller controls the extraction driver to work, the extraction driver and the gas generator can be coordinated to extract the gas in the storage and perfusion box 1 and send it into the trachea of ​​the isolated lung, thereby assisting the breathing of the isolated lung and maintaining the function of the isolated lung during operation. Generally, the extraction driver and the gas generator cooperate to deliver gas into the trachea of ​​the isolated lung at a frequency generally lower than the normal breathing frequency of the isolated lung. After the isolated lung breathes, the gas is directly discharged into the storage and perfusion box 1, thus realizing the recycling of the gas.

[0054] like Figure 6 As shown, the gas generator includes a generator housing 37, a gas extraction elastic ring 38 arranged in the generator housing 37, and a filter air inlet 39 arranged at the bottom of the generator housing 37; the extraction driver can drive the gas extraction elastic ring 38 to reciprocate in the generator housing 37. When the gas extraction elastic ring 38 reciprocates in the generator housing 37, the gas in the storage and perfusion box 1 can enter the generator housing 37 through the filter air inlet 39, and can make the gas in the generator housing 37 enter the trachea of ​​the ex vivo lung through the gas filter 40 and the tracheal tube 41.

[0055] In an embodiment of the present invention, the gas generator can take the form of an existing commonly used foot-operated air pump. Specifically, the gas generator includes a generator housing 37, a gas extraction elastic ring 38 arranged in the generator housing, and a filter air inlet 39. The filter air inlet 39 is an air inlet for setting a gas filter membrane. The gas extraction elastic ring 38 can reciprocate in the generator housing 37. The length direction of the gas extraction elastic ring 38 is consistent with the length direction of the generator housing 37. The generator housing 37 can generally be made of a flexible membrane, which can not only realize the gas extraction elastic ring 38 to be accommodated in the generator housing 37, but also realize the reciprocating motion following the gas extraction elastic ring 38. The gas extraction elastic ring 38 can generally adopt a spring ring. When the gas extraction elastic ring 38 is compressed in the generator housing 37, negative pressure can be generated in the generator housing 37, and the gas in the storage and perfusion box 1 can enter the generator housing 37 through the filter air inlet 39; when the gas extraction elastic ring 38 is reset, the gas in the generator housing 37 can enter the gas filter 40. The gas filter 40 can adopt the existing commonly used gas filtering form, such as a carbon dioxide absorbent.

[0056] After being fully filtered by the gas filter 40, the gas enters the trachea of ​​the isolated lung through the tracheal tube 41, ensuring the cleanliness of the gas entering the trachea of ​​the isolated lung. Generally, the diameter of the tracheal tube 41 can be smaller than the trachea of ​​the isolated lung, so that after the isolated lung breathes, the breathed gas or the unbreathed gas can be directly discharged into the storage and perfusion box 1 through the gap between the tracheal tube 41 and the trachea of ​​the isolated lung. In specific implementation, an exhaust valve 43 can also be provided on the tracheal tube 41, and the exhaust valve 43 can be a commonly used overflow valve, that is, when there is more gas in the generator housing 37, the excess gas can be discharged through the exhaust valve 43, or the gas in the airway of the isolated lung can be discharged through the exhaust valve 43 through the exhaust valve 43, so as to support the breathing process of the isolated lung.

[0057] When the gas generator adopts the above-mentioned structural form, the extraction driver includes an extraction elastic ring driver 13 and an extraction elastic ring driver plate 14. The extraction elastic ring driver 13 can be in the form of a linear motor or the like. The extraction elastic ring driver plate 14 is connected to the output end of the extraction elastic ring driver 13. The extraction elastic ring driver 13 and the extraction elastic ring driver plate 14 cooperate to realize the reciprocating motion of the driving gas extraction elastic ring 38. The extraction elastic ring driver 13 is installed in the second box cover 11, and the extraction elastic ring driver plate 14 is located directly above the gas extraction elastic ring 38. The specific form and driving process of the extraction elastic ring driver 13 and the extraction elastic ring driver plate 14 cooperating to drive the gas extraction elastic ring 38 are similar to the specific form and driving process of the above-mentioned perfusion elastic ring driver 9 and the perfusion elastic ring driver plate 10 driving the perfusion drive elastic ring 33. For details, please refer to the above description, which will not be repeated here.

Claims

1. An isolated lung storage and perfusion device, comprising a storage and perfusion box (1) that can be used for isolated lung storage and transportation; wherein: A perfusion circulation mechanism (5) that can be adaptively connected to the isolated lung and a breathing circulation mechanism (6) that can be adaptively connected to the isolated lung are arranged in the storage perfusion box (1); the perfusion circulation mechanism (5) and the breathing circulation mechanism (6) are both electrically connected to a perfusion breathing controller in the storage perfusion box (1); and the corresponding working states of the perfusion circulation mechanism (5) and the breathing circulation mechanism (6) can be controlled by the perfusion breathing controller; After the perfusion circulation mechanism (5) is connected to the pulmonary artery and pulmonary vein of the isolated lung, the perfusion breathing controller controls the perfusion circulation mechanism (5) to continuously perfuse the connected isolated lung with perfusion liquid, and during the perfusion liquid perfusion process, the metabolic products generated by the isolated lung can be removed; the perfusion breathing controller controls the breathing circulation mechanism (6) to assist the isolated lung in breathing, so that the isolated lung can maintain a normal breathing state; The perfusion circulation mechanism (5) comprises a perfusion fluid device (23) capable of containing perfusion fluid, a perfusion driver adapted to the perfusion fluid device (23), and a first pulmonary vein connecting tube (15), a second pulmonary vein connecting tube (16), a first pulmonary artery connecting tube (29), and a second pulmonary artery connecting tube (30) capable of communicating with the perfusion fluid device (23); The first pulmonary vein connecting tube (15) and the second pulmonary vein connecting tube (16) can be connected to the pulmonary vein adapter, and the first pulmonary artery connecting tube (29) and the second pulmonary artery connecting tube (30) can be connected to the pulmonary artery adapter. The perfusion fluid in the perfusion fluid device (23) can be driven by the perfusion driver to enter the pulmonary artery of the isolated lung via the first pulmonary artery connecting tube (29) and the second pulmonary artery connecting tube (30), and the perfusion fluid entering the isolated lung can be returned to the perfusion fluid device (23) via the first pulmonary vein connecting tube (15) and the second pulmonary vein connecting tube (16), so as to realize continuous perfusion fluid perfusion circulation of the isolated lung; It also includes a perfusion liquid outlet pipe (26) and a perfusion liquid inlet pipe (19) that can be adapted to be connected to the perfusion liquid device (23), one end of the perfusion liquid inlet pipe (19) is connected to the perfusion liquid device (23), the other end of the perfusion liquid inlet pipe (19) is connected to and communicates with the first pulmonary vein connecting pipe (15) and the second pulmonary vein connecting pipe (16), one end of the perfusion liquid outlet pipe (26) is connected to the perfusion liquid device (23), and the other end of the perfusion liquid outlet pipe (26) is connected to and communicates with the first pulmonary artery connecting pipe (29) and the second pulmonary artery connecting pipe (30); A filter remover (25) for removing metabolites in the isolated lung is provided on the perfusion outlet pipe (26), and a gas separator for discharging gas in the perfusion liquid is provided on the perfusion inlet pipe (19); A liquid outlet one-way valve (24) is arranged in the end of the perfusion liquid outlet pipe (26) adjacent to the perfusion liquid device (23), and a liquid inlet one-way valve (22) is arranged in the end of the perfusion liquid inlet pipe (19) adjacent to the perfusion liquid device (23); The perfusion liquid device (23) comprises a perfusion liquid container (32) capable of containing perfusion liquid, and a perfusion driving elastic ring (33) arranged at the upper inner portion of the perfusion liquid container (32); the perfusion driving elastic ring (33) is adaptively connected to the perfusion liquid container (32); the perfusion driving elastic ring (33) can be driven by a perfusion driver to reciprocate in the perfusion liquid container (32); when the perfusion driving elastic ring (33) reciprocates in the perfusion liquid container (32), the perfusion liquid in the perfusion liquid inlet pipe (19) can enter the perfusion liquid receiving chamber (34) at the lower portion of the perfusion liquid container (32), and the perfusion liquid in the perfusion liquid receiving chamber (34) can enter the perfusion liquid outlet pipe (26); The gas separator comprises a plurality of exhaust holes arranged on the perfusion inlet pipe (19) and a water-blocking and breathable membrane (21) arranged in the exhaust holes, and the filter remover (25) comprises an adsorption filter membrane body (36) capable of adsorbing and separating the metabolites; The breathing circulation mechanism (6) comprises a gas generator capable of extracting gas stored in a perfusion box (1), an extraction driver capable of driving the gas generator, and a gas filter (40) capable of filtering the gas extracted by the gas generator, the gas outlet of the gas filter (40) being provided with a tracheal tube (41) capable of fitting with the trachea of ​​an isolated lung, the extraction driver being electrically connected to a perfusion breathing controller, and the perfusion breathing controller being capable of controlling the extraction driver to drive the gas generator to enter a desired working state; When the perfusion breathing controller controls the extraction driver to work, the extraction driver and the gas generator can cooperate to extract the gas in the storage perfusion box (1) and send it into the trachea of ​​the isolated lung, so as to assist the breathing of the isolated lung and maintain the function of the isolated lung during operation; the extraction driver and the gas generator cooperate to send the gas into the trachea of ​​the isolated lung at a frequency lower than the normal breathing frequency of the isolated lung, and the gas after the isolated lung breathes is directly discharged into the storage perfusion box (1), so as to realize the recycling of the gas.

2. The isolated lung storage and perfusion device according to claim 1, characterized in that: A heat insulation layer (2) is arranged in the storage and perfusion box (1), and a storage and transportation refrigeration source capable of providing a low-temperature environment required for storage and transportation of ex vivo lungs is also arranged in the storage and perfusion box (1).

3. The isolated lung storage and perfusion device according to claim 2, characterized in that: The storage and transport cooling source comprises ice cubes placed in the storage and perfusion box (1) or a semiconductor refrigerator capable of achieving working cooling. The semiconductor refrigerator is connected to a perfusion breathing controller that controls the working state of the semiconductor refrigerator. The perfusion breathing controller can collect the temperature in the storage and perfusion box (1) in real time through a temperature sensor. The perfusion breathing controller can make the storage and perfusion box (1) reach a temperature environment for ex vivo lung transport through the temperature sensor and the semiconductor refrigerator.

4. The isolated lung storage and perfusion device according to claim 1 is characterized in that: A first connecting tube clamp (17) is arranged on the first pulmonary vein connecting tube (15), a second connecting tube clamp (18) is arranged on the second pulmonary vein connecting tube (16), a third connecting tube clamp (27) is arranged on the first pulmonary artery connecting tube (29), and a fourth connecting tube clamp (28) is arranged on the second pulmonary artery connecting tube (30).

5. The isolated lung storage and perfusion device according to claim 1, characterized in that: The gas generator comprises a generator housing (37), a gas extraction elastic ring (38) arranged in the generator housing (37), and a filter air inlet (39) arranged at the bottom of the generator housing (37); the extraction driver can drive the gas extraction elastic ring (38) to reciprocate in the generator housing (37); when the gas extraction elastic ring (38) reciprocates in the generator housing (37), the gas in the storage and perfusion box (1) can enter the generator housing (37) through the filter air inlet (39), and the gas in the generator housing (37) can enter the trachea of ​​the isolated lung through a gas filter (40) and a tracheal tube (41).

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