Integrated Device for Batch Preparation of Chromosomes

Abstract

The invention discloses an integrated device for batch preparation of chromosomes, and belongs to the field of chromosome preparation equipment. The integrated device for batch preparation of chromosomes can realize integrated automatic sample addition and liquid discharge, and effectively avoids the harm to human body caused by direct contact of operators with irritating chemical reagents. In the process of shaking and evenly shaking the sample, there is always a point in the low-point control ring that is lower, and the centrifugal test tube always leans on the lower position due to gravity. At the same time, under the setting of the biased weight control ball, the biased weight moving ball is in an unbalanced state of being heavy on the left and light on the right, so that it is always in a moving state during shaking, so that the lowest point on the low-point control ring is constantly changed, so that the centrifugal test tube is likely to keep in contact with the low-point control ring and move with the change of the lower point, thereby effectively avoiding the centrifugal test tube from being damaged due to point contact collision with the edge of the slot, and thereby effectively protecting the sample from accidental loss due to shaking.

Description

Technical Field

[0001] The present invention relates to the field of chromosome preparation equipment, and more specifically, to an integrated device for batch preparation of chromosomes. Background Art

[0002] Cytogenetic techniques diagnose possible chromosomal defects by analyzing chromosomes under a microscope. Clinically, chromosome preparation requires multiple steps such as culturing cells first, then blocking, harvesting, hypotonic treatment, fixation, drying, aging, enzyme digestion, and banding before chromosomes can be analyzed under a microscope to determine whether there are chromosomal abnormalities. This traditional method has been used until now. Harvesting cultured cells is an important daily work process in cytogenetic laboratories. The basic procedures of this process are as follows: transfer the cultured specimen into a centrifuge tube; after low-speed centrifugation, aspirate the supernatant and discard it; add hypotonic solution and let it act for 30 minutes, then add fixative for pre-fixation; after centrifugation, aspirate the supernatant, add fresh fixative and mix well for the first fixation; centrifuge again, aspirate the supernatant, add fresh fixative and mix well for the second fixation, and repeat this 3 times; finally, cell harvesting is completed. As can be seen from the above, there are multiple repeated operations such as aspirating liquid, adding liquid, mixing, and centrifuging during the process of harvesting chromosomes. Each step of the operation is completed one by one for each individual specimen manually, with cumbersome steps and time-consuming and laborious processes.

[0003] Regarding manual operation, there are the following hidden dangers: when manually adding samples and sampling operations, there are individual differences in the amount of residual liquid after each removal of the supernatant and the amount of fresh liquid added each time, which inevitably leads to batch differences, thereby having a certain impact on cell harvesting and causing unstable results. When manual operation is used to process the sample, it is necessary to use a pipette for multiple operations, which takes a long time and has a large workload, causing excessive fatigue of the personnel. In addition, when adding samples manually, it is necessary to directly contact various chemical reagents, some of which have toxic effects or irritating odors, which will cause certain harm to the operator's body. The manual operation steps are cumbersome and independent of each other, and frequent specimen transportation will lead to specimen contamination and increased error rate. The existing waste liquid tank lacks cleaning equipment and liquid level alarm system. The waste liquid needs to be disassembled and cleaned by the staff themselves. The waste liquid has a pungent smell and is toxic, which is harmful to the operator's body and mind, and the waste liquid tank is difficult to clean, resulting in dirty residues in the waste liquid compartment, affecting the appearance; the lack of a liquid level alarm will cause the waste liquid to reflux, damage the instrument, and increase the failure rate. Therefore, in order to ensure the efficiency of chromosome acquisition, the accuracy of the data obtained, and the safety of the operators, the prior art tends to gradually tend to batch and automate the acquisition of chromosomes. However, in the process of shaking the sample in the centrifuge tube by an oscillator, the centrifuge tube usually vibrates to a certain extent with the shaking force, causing it to continuously collide with the edge of the notch of the test tube slot on the test tube rack. After long-term use, the stress structure of the collision point of the same centrifuge tube changes due to long-term expansion, and it is easy to break and be damaged during the centrifugal shaking process, resulting in accidental spillage and loss of the sample. In addition, due to the chemical reagents in the sample, there are certain hidden dangers to the safety of the surrounding staff when spilling. Summary of the invention

[0004] 1. Technical issues to be solved

[0005] In view of the problems existing in the prior art, the purpose of the present invention is to provide an integrated device for batch preparation of chromosomes, which can realize integrated automated sample addition and liquid discharge, effectively avoiding harm to the human body caused by direct contact of operators with irritating chemical reagents. In the process of shaking the sample, the unbalanced liquid in the low-point control ring and the weighted moving ball are always deformed downward under the action of gravity, making the position of this place lower. The centrifugal test tube always leans on this lower position due to the action of gravity. At the same time, under the setting of the weighted control ball, the weighted moving ball is in an unbalanced state with heavy left and light right, so that it is always in a moving state during shaking, so that the lowest point on the low-point control ring is constantly changing, so that the centrifugal test tube is likely to remain in contact with the low-point control ring and move with the change of the lower point, thereby effectively avoiding the centrifugal test tube from being damaged due to point contact collision with the edge of the slot, thereby effectively protecting the sample from accidental loss due to shaking.

[0006] 2. Technical solution

[0007] To solve the above problems, the present invention adopts the following technical solution.

[0008] An integrated device for batch preparation of chromosomes, comprising a fixed back plate, a shaking water bath device is fixedly connected to the lower end of the fixed back plate, a sampling fixing plate with a lifting mechanism is fixedly connected to the front end of the fixed back plate, the vertical plane of the control panel and the sampling fixing plate is mounted on the fixed back plate, a sample inlet and a liquid discharge port are respectively fixedly connected to the upper left and right ends of the sampling fixing plate, a reagent box and a waste liquid box are respectively arranged outside the fixed back plate, a liquid infusion pipe with a pressure regulator is connected between the liquid discharge port and the waste liquid box and between the sample inlet and the reagent box, a control panel is further mounted on the upper end of the sampling fixing plate, the pressure regulator and the lifting mechanism are both in signal connection with the control panel, a plurality of uniformly distributed sampling needles are connected to the lower end of the sampling fixing plate through an electromagnet, the liquid discharge port, the sample inlet and the plurality of sampling needles are all communicated with the inside of the sampling fixing plate, the shaking water bath device comprises a test tube rack mounted at the uppermost end, a shaker mounted below the test tube rack and a water bath box with a liquid outlet mounted below the shaker, a plurality of card slots corresponding to the sampling needles are drilled on the test tube rack, a low point control protection ring is fixedly connected to the inner wall of the card slot, a centrifuge tube is inserted into the card slot, which can realize the integration of automatic sample addition and liquid discharge, effectively avoiding the harm caused to the human body by the operator directly contacting irritating chemical reagents. During the process of shaking and mixing the sample, the balance-breaking liquid and the heavy moving ball in the low point control protection ring always deform downward under the action of gravity, so that the position of the two parts is lower. Due to the action of gravity, the centrifuge tube always leans on this lower position. At the same time, under the setting of the heavy control ball, the heavy moving ball is in an unbalanced state of left-heavy and right-light, so that it is always in a moving state during shaking, making the lowest point on the low point control protection ring constantly change, so that the centrifuge tube is likely to remain in contact with the low point control protection ring and move with the change of the lower position, thus effectively avoiding the situation that the centrifuge tube is damaged due to point contact collision with the edge of the card slot, and effectively protecting the sample from being accidentally lost due to shaking.

[0009] Furthermore, a sample addition button, a mixing button, a display screen, a water bath setting button, a sample discharge button and a program setting button are arranged on the control panel. The sample addition button is used to control the sample addition step, the mixing button is used to control the mixing step, the water bath setting button is used to control the water bath step, the sample discharge button is used to control the waste liquid discharge step, and the program setting button is used for multi-program setting, which can realize the integration of automatic sample addition and liquid discharge, effectively avoiding the harm caused to the human body by the operator directly contacting irritating chemical reagents. At the same time, it automatically controls the shaking intensity and the constant water bath temperature, effectively improving the efficiency of sample mixing.

[0010] Furthermore, the thickness of the test tube rack is 0.8 - 1.2 cm.

[0011] Furthermore, the distance between the shaker and the test tube rack is 14 - 16 cm, and the distance between the shaker and the bottom of the water bath tank is also 14 - 16 cm, which facilitates the full contact between the bottom of the centrifuge tube and the shaker under the condition of constant temperature water bath, so as to make the mixing sufficient.

[0012] Furthermore, the outer surface of the cross-section of the low-point control ring is a vertical surface, the inner surface of the cross-section of the low-point control ring is a semi-elliptical shape arched towards the middle, and the thickness of the low-point control ring is not less than the card slot to ensure that the contact point between the centrifuge tube and the low-point control ring is the only contact point, effectively avoiding the contact between the centrifuge tube and the edge of the card slot opening during vibration, thus effectively protecting the centrifuge tube from being accidentally damaged during vibration, and effectively avoiding the accidental loss of samples caused by vibration.

[0013] Furthermore, the low-point control ring is a hollow structure, and the inside of the low-point control ring is filled with heavy moving balls and balance-breaking liquid. A sliding groove is drilled at the inner bottom end of the low-point control ring, and the heavy moving balls are slidably connected to the sliding groove. Under the action of gravity, the balance-breaking liquid and the heavy moving balls always move downward in the vertical direction, so that the two always deform downward at the part of the low-point control ring, making the plane of the low-point control ring at this part lower than other parts. Therefore, the centrifuge tube always leans on this lower position under the action of gravity.

[0014] Furthermore, the balance-breaking liquid is a non-corrosive liquid, such as water. The inner walls of the low-point control ring and the heavy moving balls are coated with a hydrophobic coating, effectively avoiding the infiltration of the balance-breaking liquid into the low-point control ring, and further effectively avoiding the situation that the balance-breaking liquid becomes less and less during long-term use. The filling weight of the balance-breaking liquid in the low-point control ring is 1.5 - 2 times the weight of the heavy moving balls, effectively ensuring that the total weight of the balance-breaking liquid will not be too much, thus effectively avoiding the situation that the lower end of the low-point control ring deforms downward as a whole.

[0015] Furthermore, the weighted moving ball is a hollow structure, and a plurality of evenly distributed weighted control balls are fixedly connected inside the weighted moving ball. The weighted control ball includes a draw rope fixedly connected to the inner wall of the weighted moving ball and a left weighted ball connected to the end of the draw rope. Through the setting of the weighted control ball, the weighted moving ball is in an unbalanced state when used. When the oscillator starts working and generates vibration force, it is difficult for the weighted moving ball to be in a static state. Under the action of the unbalanced force, the weighted moving ball slides along the sliding groove during the oscillation process. Since the centrifugal test tube rests on the lower part of the low-point control protection ring, during the process of sample oscillation and even shaking, the position of the weighted moving ball keeps moving, and the lowest point on the low-point control protection ring keeps changing, so that the centrifugal test tube is always in contact with the low-point control protection ring and moves with the change of the lowest point, thereby effectively avoiding the centrifugal test tube from being damaged due to point contact collision with the edge of the slot during the vibration process, thereby effectively avoiding the accidental loss of the sample due to the vibration.

[0016] Furthermore, the length of the pull rope is greater than the radius of the hollow part inside the eccentrically weighted moving ball and smaller than the inner diameter of the hollow part, so that the left eccentrically weighted ball connected to the end of the pull rope cannot be stationary in the middle of the eccentrically weighted moving ball, thereby making the eccentrically weighted moving ball always in an unbalanced state, effectively preventing the eccentrically weighted moving ball from being stationary due to being in a balanced state.

[0017] Furthermore, a side weighted ball is embedded in the outer end of the left-biased weighted ball, which is biased to the left side of the extension line of the pull rope, and the offset angle of the side weighted ball to the left along the extension line of the side weighted ball is 30-60°, so that the biased moving ball is overall biased to the left side and light to the right side, thereby effectively ensuring that the biased moving ball is in an unbalanced state, and then effectively ensuring that the biased moving ball is always in a moving state during the process of the oscillator shaking the sample, thereby making the low-point control ring better protect the centrifuge test tube.

[0018] 3. Beneficial effects

[0019] Compared with the prior art, the advantages of the present invention are:

[0020] (1) This solution can achieve the integration of automated sample addition and liquid drainage, effectively avoiding the harm caused to the human body by operators directly contacting irritating chemical reagents. During the process of shaking and mixing the sample, under the action of gravity, the balance-breaking liquid in the low-point control ring and the heavy moving ball always cause downward deformation in the part where they are located, making the position there lower. Due to gravity, the centrifuge tube always leans on this lower position. At the same time, with the setting of the heavy control ball, the heavy moving ball is in an unbalanced state with the left side heavier and the right side lighter, making it always in a moving state during shaking, causing the lowest point on the low-point control ring to constantly change, making the centrifuge tube likely to move with the change of the lower position while remaining in contact with the low-point control ring, thus effectively avoiding the situation where the centrifuge tube is damaged due to point contact collision with the edge of the card slot, and effectively protecting the sample from being accidentally lost due to shaking.

[0021] (2) The control panel is provided with a sample addition button, a mixing button, a display screen, a water bath setting button, a sample drainage button, and a program setting button. The sample addition button is used to control the sample addition step, the mixing button is used to control the mixing step, the water bath setting button is used to control the water bath step, the sample drainage button is used to control the waste liquid drainage step, and the program setting button is used for multi-program setting. It can achieve the integration of automated sample addition and liquid drainage, effectively avoiding the harm caused to the human body by operators directly contacting irritating chemical reagents. At the same time, it automatically controls the shaking intensity and the constant water bath temperature, effectively improving the efficiency of sample mixing.

[0022] (3) The thickness of the test tube rack is 0.8 - 1.2 cm, the distance between the shaker and the test tube rack is 14 - 16 cm, and the distance between the shaker and the bottom of the water bath box is also 14 - 16 cm, which is convenient for the bottom of the centrifuge tube to fully contact the shaker under the condition of constant temperature water bath, so as to make the mixing sufficient.

[0023] (4) The outer surface of the cross-section of the low-point control ring is a vertical surface, the inner surface of the cross-section of the low-point control ring is a semi-elliptical shape arched towards the middle, and the thickness of the low-point control ring is not less than the card slot to ensure that the contact point between the centrifuge tube and the low-point control ring is the only contact point, effectively avoiding the centrifuge tube from contacting the edge of the card slot during shaking, thus effectively protecting the centrifuge tube from being accidentally damaged during shaking, and effectively avoiding the situation where the sample is accidentally lost due to shaking.

[0024] (5) The low-point control ring is a hollow structure, and the inside of the low-point control ring is filled with a heavy moving ball and a balance-breaking liquid. A sliding groove is drilled at the inner bottom end of the low-point control ring, and the heavy moving ball is slidably connected to the sliding groove. Under the action of gravity, the balance-breaking liquid and the heavy moving ball always move downward in the vertical direction, causing downward deformation in the part of the low-point control ring where they are located, making the plane of the low-point control ring at this place lower than other parts, so that the centrifuge tube always leans on this lower position due to gravity.

[0025] (6) The unbalancing liquid is a non-corrosive liquid, such as water. The inner wall of the low point control ring and the weighted moving ball is coated with a hydrophobic coating, which effectively prevents the unbalancing liquid from penetrating into the low point control ring, thereby effectively preventing the unbalancing liquid from becoming less and less during long-term use. The filling weight of the unbalancing liquid in the low point control ring is 2 / 3 of the thickness of the weighted moving ball, and the effective amount is 1.5-2 times, so that the overall weight of the unbalancing liquid will not be too much, thereby effectively preventing the overall downward deformation of the lower end of the low point control ring.

[0026] (7) The weighted moving ball is a hollow structure, and a plurality of evenly distributed weighted control balls are fixedly connected inside the weighted moving ball. The weighted control balls include a drawstring fixedly connected to the inner wall of the weighted moving ball and a left weighted ball connected to the end of the drawstring. The weighted control ball is set so that the weighted moving ball is in an unbalanced state when used. When the oscillator starts working and generates vibration force, it is difficult for the weighted moving ball to be in a static state. Under the action of the unbalanced force, the weighted moving ball slides along the sliding groove during the oscillation process. Since the centrifugal test tube rests on the lower part of the low-point control ring, the lowest point on the low-point control ring changes continuously during the oscillation and shaking of the sample, so that the centrifugal test tube is always in contact with the low-point control ring and moves with the change of the lowest point, thereby effectively avoiding the centrifugal test tube from being damaged due to point contact collision with the edge of the slot during the vibration process, thereby effectively avoiding the accidental loss of the sample due to the vibration.

[0027] (8) The length of the pull rope is greater than the radius of the hollow part of the eccentric moving ball and smaller than the inner diameter of the hollow part, so that the left eccentric moving ball connected to the end of the pull rope cannot be stationary in the middle of the eccentric moving ball, thereby making the eccentric moving ball always in an unbalanced state, effectively preventing the eccentric moving ball from being stationary due to being in a balanced state.

[0028] (9) A side weighting ball is embedded at the outer end of the left biased weight ball. The side weighting ball is biased to the left side of the extension line of the pull rope, and the side weighting ball is offset to the left at an angle of 30-60° along the extension line of the side weighting ball, so that the biased weight moving ball is biased to the left side and biased to the right side, thereby effectively ensuring that the biased weight moving ball is in an unbalanced state, and further effectively ensuring that the biased weight moving ball is always in a moving state during the process of the oscillator shaking the sample, so that the low point control ring has a better protective effect on the centrifuge test tube. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 It is a schematic structural diagram of the front side of the present invention;

[0030] Figure 2 It is a structural schematic diagram of the oscillating water bath device of the present invention;

[0031] Figure 3 Schematic diagram of the structure of the centrifuge tube of the present invention when it relies on the lower point of the lower point protection ring;

[0032] Figure 4 Schematic diagram of the structure of the lower point protection ring of the present invention;

[0033] Figure 5 Schematic diagram of the cross-sectional structure of the lower point protection ring of the present invention;

[0034] Figure 6 Schematic diagram of the structure of the heavy moving ball of the present invention.

[0035] Explanation of the reference numerals in the figure:

[0036] 1 Fixed back plate, 2 Sampling fixing plate, 3 Oscillating water bath device, 31 Test tube rack, 311 Card slot, 32 Oscillator, 33 Water bath tank, 4 Reagent box, 5 Waste liquid tank, 6 Drainage port, 7 Sampling port, 81 Sampling needle, 82 Centrifuge tube, 9 Control panel, 10 Lower point protection ring, 11 Heavy moving ball, 12 Sliding groove, 13 Pulling rope, 14 Left heavy ball, 15 Side heavy ball. Detailed implementation manners

[0037] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention; obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0038] In the description of the present invention, it should be noted that the orientation or positional relationship indicated by the terms "upper", "lower", "inner", "outer", "top / bottom end", etc. is based on the orientation or positional relationship shown in the accompanying drawings, and is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation to the present invention. In addition, the terms "first" and "second" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance.

[0039] In the description of the present invention, it should be noted that unless otherwise clearly specified and limited, the terms "installed", "provided with", "sheathed / connected", "connected", etc. should be understood in a broad sense. For example, "connected" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific situations.

[0040] Example 1:

[0041] Please refer to Figure 1 , the integrated device for batch preparation of chromosomes, including a fixed backboard 1, a shaking water bath device 3 fixedly connected to the lower end of the fixed backboard 1, a sampling fixing plate 2 with a lifting mechanism fixedly connected to the front end of the fixed backboard 1, a control panel 9 and the vertical surface of the sampling fixing plate 2 are installed on the fixed backboard 1. Sampling ports 7 and drainage ports 6 are respectively fixedly connected to the upper left and right ends of the sampling fixing plate 2. A reagent box 4 and a waste liquid box 5 are respectively arranged outside the fixed backboard 1. Liquid infusion pipes with pressure regulators are connected between the drainage port 6 and the waste liquid box 5 and between the sampling port 7 and the reagent box 4. A control panel 9 is also installed at the upper end of the sampling fixing plate 2. The pressure regulator and the lifting mechanism are both in signal connection with the control panel 9. The control panel 9 is provided with a sample addition button, a mixing button, a display screen, a water bath setting button, a sample discharge button, and a program setting button. The sample addition button is used to control the sample addition step, the mixing button is used to control the mixing step, the water bath setting button is used to control the water bath step, the sample discharge button is used to control the waste liquid discharge step, and the program setting button is used for multi-program setting, which can realize the integration of automatic sample addition and liquid discharge, effectively avoiding the harm caused to the human body by the operator directly contacting irritating chemical reagents. At the same time, the oscillation intensity and the constant water bath temperature are automatically controlled, effectively improving the efficiency of sample mixing. The lower end of the sampling fixing plate 2 is connected with a plurality of evenly distributed sampling needles 81 through an electromagnet. The drainage port 6, the sampling port 7, and the plurality of sampling needles 81 are all communicated with the inside of the sampling fixing plate 2.

[0042] Please refer to Figure 2 , the shaking water bath device 3 includes a test tube rack 31 installed at the uppermost end, a shaker 32 installed below the test tube rack 31, and a water bath box 33 with a liquid outlet installed below the shaker 32. A plurality of card slots 311 corresponding to the sampling needles 81 are drilled on the test tube rack 31. A low-point control ring 10 is fixedly connected to the inner wall of the card slot 311. The thickness of the test tube rack 31 is 0.8 - 1.2 cm. The distance between the shaker 32 and the test tube rack 31 is 14 - 16 cm. The distance between the shaker 32 and the bottom of the water bath box 33 is also 14 - 16 cm, which is convenient for the bottom of the centrifuge tube 82 to fully contact the shaker 32 under the condition of constant temperature water bath, so as to make the mixing sufficient.

[0043] Please refer to Figures 3 - 5, a centrifuge tube 82 is inserted into the card slot 311. The outer surface of the cross-section of the low-point control ring 10 is a vertical plane, and the inner surface of the cross-section of the low-point control ring 10 is a semi-elliptical shape arched towards the middle. Moreover, the thickness of the low-point control ring 10 is not less than 2 / 3 of the thickness of the card slot 311, effectively ensuring that the contact point between the centrifuge tube 82 and the low-point control ring 10 is the only contact point, effectively preventing the centrifuge tube 82 from contacting the edge of the card slot 311 during oscillation, thereby effectively protecting the centrifuge tube 82 from being accidentally damaged during oscillation, and effectively avoiding the occurrence of accidental loss of samples due to oscillation. The low-point control ring 10 is a hollow structure, and the inside of the low-point control ring 10 is filled with heavy moving balls 11 and balance-breaking liquid. A sliding groove 12 is drilled at the inner bottom end of the low-point control ring 10, and the heavy moving balls 11 are slidably connected to the sliding groove 12. Under the action of gravity, the balance-breaking liquid and the heavy moving balls 11 always move downward in the vertical direction, causing the two to always deform downward in the part of the low-point control ring 10, making the plane of the low-point control ring 10 at this part lower than other parts. Therefore, the centrifuge tube 82 always leans on this lower position under the action of gravity. The balance-breaking liquid is a non-corrosive liquid, such as water. The inner walls of the low-point control ring 10 and the heavy moving balls 11 are coated with a hydrophobic coating, effectively preventing the balance-breaking liquid from seeping into the low-point control ring 10, and further effectively avoiding the situation that the balance-breaking liquid becomes less and less during long-term use. Moreover, the filling weight of the balance-breaking liquid in the low-point control ring 10 is 1.5 - 2 times the weight of the heavy moving balls 11, so that the total weight of the balance-breaking liquid will not be too much, effectively preventing the overall downward deformation of the lower end of the low-point control ring 10 from occurring.

[0044] Please refer to Figure 6The weighted moving ball 11 is a hollow structure, and a plurality of evenly distributed weighted control balls are fixedly connected inside the weighted moving ball 11. The weighted control balls include a drawstring 13 fixedly connected to the inner wall of the weighted moving ball 11 and a left weighted ball 14 connected to the end of the drawstring 13. By setting the weighted control balls, the weighted moving ball 11 is in an unbalanced state when used. When the oscillator 32 starts working and generates vibration force, it is difficult for the weighted moving ball 11 to be in a static state. Under the action of the unbalanced force, the weighted moving ball 11 is The weighted ball 11 slides along the sliding groove 12 during the oscillation process. Since the centrifugal test tube 82 rests on the lower part of the low-point control ring 10, during the process of sample oscillation and even shaking, the position of the weighted ball 11 is constantly moving, and the lowest point on the low-point control ring 10 is constantly changing, so that the centrifugal test tube 82 is always in contact with the low-point control ring 10 and moves with the change of the lowest point, thereby effectively avoiding the centrifugal test tube 82 from being damaged due to point contact collision with the edge of the slot 311 during the vibration process, thereby effectively avoiding the accidental loss of the sample due to the vibration. The length of the pull rope 13 is greater than the radius of the hollow part of the weighted ball 11 and smaller than the inner diameter of the hollow part, so that the left weighted ball 14 connected to the end of the pull rope 13 cannot be stationary in the middle of the weighted ball 11, thereby making the weighted ball 11 always in an unbalanced state, effectively avoiding the situation where the weighted ball 11 is stationary due to being in a balanced state. The outer end of the left weighted ball 14 is inlaid with a side weighted ball 15, the side weighted ball 15 is biased to the left side of the extension line of the pull rope 13, and the offset angle of the side weighted ball 15 to the left along the extension line of the side weighted ball 15 is 30-60 degrees, so that the weighted moving ball 11 is heavier on the left side and lighter on the right side as a whole, thereby effectively ensuring that the weighted moving ball 11 is in an unbalanced state, and further effectively ensuring that the weighted moving ball 11 is always in a moving state during the process of the oscillator 32 working to oscillate and shake the sample, so that the low point control ring 10 has a better protective effect on the centrifuge test tube 82.

[0045] The automatic sample addition and liquid discharge can be integrated, and the operator is effectively prevented from being directly exposed to irritating chemical reagents and causing harm to the human body. In the process of shaking the sample, the unbalanced liquid in the low-point control ring 10 and the weighted moving ball 11 are always deformed downward under the action of gravity, making the position of the place lower. The centrifugal test tube 82 always leans on the lower position due to the action of gravity. At the same time, under the setting of the weighted control ball, the weighted moving ball 11 is in an unbalanced state of being heavy on the left and light on the right, so that it is always in a moving state during the vibration, so that the lowest point on the low-point control ring 10 is constantly changing, so that the centrifugal test tube 82 is likely to remain in contact with the low-point control ring 10 and move with the change of the lower point, thereby effectively avoiding the centrifugal test tube 82 from being damaged due to point contact collision with the edge of the slot 311, thereby effectively protecting the sample from accidental loss due to vibration.

[0046] The above are only the preferred specific embodiments of the present invention. However, the protection scope of the present invention is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present invention, according to the technical solution and its improved concept of the present invention, making equivalent substitutions or changes, shall be covered by the protection scope of the present invention.

Claims

1. An integrated device for batch preparation of chromosomes, comprising a fixed backboard (1), a shaking water bath device (3) is fixedly connected to the lower end of the fixed backboard (1), a sampling fixing plate (2) with a lifting mechanism is fixedly connected to the front end of the fixed backboard (1), a control panel (9) and the vertical surface of the sampling fixing plate (2) are installed on the fixed backboard (1), a sampling port (7) and a drain port (6) are respectively fixedly connected to the upper left and right ends of the sampling fixing plate (2), a reagent box (4) and a waste liquid box (5) are respectively arranged outside the fixed backboard (1), a liquid infusion tube with a pressure regulator is connected between the drain port (6) and the waste liquid box (5) and between the sampling port (7) and the reagent box (4), a control panel (9) is further installed at the upper end of the sampling fixing plate (2), and the pressure regulator and the lifting mechanism are both in signal connection with the control panel (9). It is characterized in that: A plurality of uniformly distributed sampling needles (81) are connected to the lower end of the sampling fixing plate (2) through electromagnets, the drain port (6), the sampling port (7) and the plurality of sampling needles (81) are all communicated with the inside of the sampling fixing plate (2), the shaking water bath device (3) comprises a test tube rack (31) installed at the uppermost end, a shaker (32) installed below the test tube rack (31), and a water bath box (33) with a liquid outlet installed below the shaker (32), a plurality of card slots (311) corresponding to the sampling needles (81) are formed in the test tube rack (31), a low point control ring (10) is fixedly connected to the inner wall of the card slot (311), and a centrifuge test tube (82) is inserted into the card slot (311); the outer surface of the cross section of the low point control ring (10) is a vertical surface, the inner surface of the cross section of the low point control ring (10) is a semi-elliptical shape arched towards the middle, and the thickness of the low point control ring (10) is not less than 2 / 3 of the thickness of the card slot (311); the low point control ring (10) is a hollow structure, the inside of the low point control ring (10) is filled with a heavy moving ball (11) and a balance-breaking liquid, a sliding groove (12) is formed at the inner bottom end of the low point control ring (10), and the heavy moving ball (11) is slidably connected to the sliding groove (12); the heavy moving ball (11) is a hollow structure, a plurality of uniformly distributed heavy control balls are fixedly connected to the inside of the heavy moving ball (11), the heavy control ball comprises a pull rope (13) fixedly connected to the inner wall of the heavy moving ball (11) and a left heavy ball (14) connected to the end of the pull rope (13), the length of the pull rope (13) is greater than the radius of the hollow part inside the heavy moving ball (11) and less than the inner diameter of the hollow part, and a side heavy ball (15) is embedded at the outer end of the left heavy ball (14), and the side heavy ball (15) is biased to the left side of the extension line of the pull rope (13).

2. The integrated device for batch preparation of chromosomes according to claim 1, It is characterized in that: The control panel (9) is provided with a sample adding button, a mixing button, a display screen, a water bath setting button, a sample discharging button and a program setting button. The sample adding button is used to control the sample adding step, the mixing button is used to control the mixing step, the water bath setting button is used to control the water bath step, the sample discharging button is used to control the waste liquid discharging step, and the program setting button is used for multi-program setting.

3. The integrated device for batch preparation of chromosomes according to claim 1, characterized in that: the thickness of the test tube rack (31) is 0.8 - 1.2 cm.

4. The integrated device for batch preparation of chromosomes according to claim 3, characterized in that: the distance between the shaker (32) and the test tube rack (31) is 14 - 16 cm, and the shaker (32) is also 14 - 16 cm away from the bottom of the water bath tank (33).

5. The integrated device for batch preparation of chromosomes according to claim 4, characterized in that: the breaking balance liquid is a non-corrosive liquid, the inner walls of the low point protection ring (10) and the heavy moving ball (11) are coated with a hydrophobic coating, and the filling weight of the breaking balance liquid in the low point protection ring (10) is 1.5 - 2 times the weight of the heavy moving ball (11).

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