A real-time fluorescent PCR analyzer

Abstract

The application relates to a real-time fluorescent PCR analyzer, and the switch cover device comprises a power mechanism, a transmission mechanism and a motion mechanism. The power mechanism comprises an output shaft. One end of a first rocker in the transmission mechanism is rotationally connected with the output shaft, and the other end of the first rocker is rotationally connected with one end of a connecting rod. In the motion mechanism, a cover body is hingedly connected with a fixing base, a limiting hole is arranged on the fixing base, one end of a second rocker is fixedly connected with a hinged end of the cover body, and the other end of the second rocker passes through the limiting hole and is rotationally connected with the other end of the connecting rod. The switch cover device is simple and exquisite in overall structure, easy to realize, and can be conveniently applied to small detection instruments. Moreover, through automatic signal control on the power mechanism, switch cover operation on multiple instrument channels can be easily realized, control is accurate, and the detection efficiency is greatly improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of medical detection equipment, and particularly relates to a real-time fluorescent PCR analyzer. BACKGROUND

[0002] At present, various reagents are often detected in the medical field, and during the detection process, the reagent box containing the reagents needs to be frequently opened and closed. The common detection mechanism in the existing medical equipment is a manual opening and closing structure. When the number of instrument channels is large, the corresponding sample reagent types of the corresponding channels need to be recorded one by one, which is easy to confuse the channel sample types and greatly reduces the detection efficiency. SUMMARY

[0003] Therefore, it is necessary to provide a real-time fluorescent PCR analyzer to solve the problem of low detection efficiency caused by slow opening and closing speed of the manual opening and closing structure and manual operation confusion.

[0004] The embodiment of the present application provides an opening and closing device, which comprises:

[0005] A power mechanism comprising an output shaft;

[0006] A transmission mechanism comprising a first rocker and a connecting rod, one end of the first rocker being rotationally connected with the output shaft, and the other end of the first rocker being rotationally connected with one end of the connecting rod;

[0007] An action mechanism comprising a fixed seat, a second rocker and a cover, the cover being hingedly connected with the fixed seat, a limiting hole being formed in the fixed seat, one end of the second rocker being fixedly connected with the hinged end of the cover, and the other end of the second rocker being rotationally connected with the other end of the connecting rod, so that the second rocker can drive the cover to rotate relative to the fixed seat to open and close the cover.

[0008] In some embodiments, a containing cavity is formed in the fixed seat, and a plurality of abutting portions connected in a loop are protrudingly arranged on the surface of the cover facing the fixed seat, the abutting portions being accommodated in the containing cavity when the cover is closed with the fixed seat.

[0009] In some embodiments, a locking portion is further protrudingly arranged on the surface of the cover facing the fixed seat, the locking portion being arranged perpendicularly to the surface of the cover, and the locking portion being locked with the fixed seat when the cover is closed with the fixed seat.

[0010] In some embodiments, the first rocker rotates relative to the connecting rod and the second rocker rotates relative to the connecting rod in the same plane.

[0011] In some embodiments, the length of the first rocker is equal to the length of the second rocker, and the length of the first rocker is less than the length of the connecting rod.

[0012] In some embodiments, the first rocker and the connecting rod are connected by a rotating shaft, the rotating shaft comprises a first shaft segment and a second shaft segment, the first shaft segment and the second shaft segment have different diameters, the first rocker is provided with a first through hole matched with the first shaft segment, and the connecting rod is provided with a second through hole matched with the second shaft segment.

[0013] In some embodiments, the diameter of the second shaft segment is greater than that of the first shaft segment.

[0014] In some embodiments, the first through hole is provided with an internal thread, and the first shaft segment is provided with an external thread matched with the internal thread.

[0015] In some embodiments, the rotating shaft is further provided with a third shaft segment at an end of the second shaft segment away from the first shaft segment, and the diameter of the third shaft segment is greater than that of the second shaft segment, so as to avoid the disconnection of the connecting rod and the second shaft segment.

[0016] The embodiment of the present application also provides a real-time fluorescent PCR analyzer comprising the switch cover device.

[0017] The switch cover device.

[0018] The switch cover device and the real-time fluorescent PCR analyzer provided by the present application utilize the output shaft to drive the first rocker to rotate, and the rotation of the first rocker is transmitted to the second rocker through the connecting rod, the second rocker is rotatably connected with the connecting rod through the limiting hole of the fixed seat, and the hinged end of the cover body is fixedly connected with the second rocker, so that the hinged end of the cover body is driven by the second rocker to rotate relative to the fixed seat, thereby realizing the functions of opening and closing the cover. The overall structure is simple and exquisite, easy to realize, and can be conveniently applied to small detection instruments. Moreover, through automatic signal control of the power mechanism, the cover opening and closing operation of multiple instruments can be easily realized, the control is accurate, and the detection efficiency is greatly improved. BRIEF DESCRIPTION OF DRAWINGS

[0019] Fig. 1 is a schematic diagram of the overall structure of the switch cover device in the embodiment of the present application;

[0020] Figure 2 Fig. 2 is an exploded view of the overall structure of the switch cover device in the embodiment of the present application;

[0021] Figure 3 Fig. 3 is a perspective view of the overall structure of the switch cover device in the first state in the embodiment of the present application;

[0022] Figure 4 Fig. 4 is a perspective view of the overall structure of the switch cover device in the second state in the embodiment of the present application;

[0023] Figure 5 Fig. 5 is a perspective view of the overall structure of the switch cover device in the third state in the embodiment of the present application. DETAILED DESCRIPTION

[0024] The preferred embodiments of the present application will be described in detail below with reference to the drawings, in which the preferred embodiments of the present application constitute a part of the present application and are used to explain the principles of the present application, but are not used to limit the scope of the present application.

[0025] Please refer to Figures 1 to 5 The switch cover device 10 provided by the embodiments of the present application comprises a power mechanism 100, a transmission mechanism 200 and a motion mechanism 300.

[0026] The power mechanism 100 comprises an output shaft 110. The power mechanism 100 is a mechanism commonly used in the technical field to provide power output, which can convert different energies in nature into mechanical energy to do work and output through the output shaft 110. The power mechanism 100 is divided into several categories such as wind power machinery, water power machinery and thermal power engine. In the present embodiment, the power mechanism 100 can adopt a rudder machine, which is a position or angle servo driver suitable for control systems that need to change angles constantly and can be maintained, and mainly comprises a circuit board, a driving motor, a speed reducer, a position detection element and other components.

[0027] The transmission mechanism 200 is used to transmit the power provided by the power mechanism 100 to the motion mechanism 300. The transmission mechanism 200 comprises a first rocker 210 and a connecting rod 220, one end of the first rocker 210 is rotationally connected with the output shaft 110, and the other end of the first rocker 210 is rotationally connected with one end of the connecting rod 220. The first rocker 210 is a connecting rod that can only swing within a certain angle range, such as less than 90 degrees. One end of the first rocker 210 will rotate around the output shaft 110 under the driving of the output shaft 110, and the other end of the first rocker 210 is connected with the connecting rod 220, so that the connecting rod 220 will swing under the pushing of the other end of the first rocker 210.

[0028] The action mechanism 300 is used for opening and closing the cover. The action mechanism 300 comprises a fixed seat 310, a second rocker 320 and a cover 330. The cover 330 is hinged to the fixed seat 310. The fixed seat 310 is provided with a limiting hole 311 and a containing cavity 312. One end of the second rocker 320 is fixedly connected to the hinged end of the cover 330. The other end of the second rocker 320 penetrates through the limiting hole 311 and is rotatably connected to the other end of the connecting rod 220. Thus, the second rocker 320 can drive the cover 330 to rotate relative to the fixed seat 310, so as to realize the opening and closing of the cover by the cooperation of the cover 330 and the containing cavity 312. In this way, the hinged end of the cover 330 rotates relative to the fixed seat 310 under the driving of the second rocker 320. The position of the second rocker 320 in the limiting hole 311 and the rotation angle of the first rocker 210 and the connecting rod 220 can be adjusted to adjust the rotation range of the cover 330 on the fixed seat 310, so as to realize the closing or opening of the cover.

[0029] The opening and closing device 10 provided by the application drives the first rocker 210 to rotate by the output shaft 110, and transmits the rotation of the first rocker 210 to the second rocker 320 through the connecting rod 220. The second rocker 320 is rotatably connected to the connecting rod 220 through the limiting hole 311 of the fixed seat 310, and the hinged end of the second rocker 320 is fixedly connected to the cover 330. In this way, the hinged end of the cover 330 rotates relative to the fixed seat 310 under the driving of the second rocker 320, so as to realize the opening and closing functions. The overall structure is simple and exquisite, easy to realize, and can be conveniently applied to small detection instruments. Moreover, the opening and closing operation of multiple instrument channels can be easily realized by automatically controlling the power mechanism 100, the control is accurate, and the detection efficiency is greatly improved.

[0030] The cover 330 comprises a body 330a and a clamping portion 331 protruding from the surface of the body 330a towards the fixed seat 310. In this embodiment, the body 330a and the clamping portion 331 are integrally formed. When the cover 330 is rotated to be closed with the fixed seat 310, the clamping portion 331 is clamped into the containing cavity 312. Figures 3 to 5 The overall structure perspective views of the opening and closing device 10 in the first state, the second state and the third state when the cover 330 is rotated to different positions on the fixed seat 310 are shown in FIGS. 1, 2 and 3, respectively. Figure 3 In FIG. 1, the cover 330 is rotated to abut against the surface of the fixed seat 310. The containing cavity 312 is blocked by the clamping portion 331, and the cover is in the closed state. Figure 4 In FIG. 2, the cover 330 is rotated to be separated from the fixed seat 310. The cover 330 is in the intermediate position, and the containing cavity 312 is partially opened. Figure 5In some embodiments, the cover 330 is locked with the fixed base 310 when the kit is not used any more. In some embodiments, the other end of the cover 330 is provided with a locking part 332 which is perpendicular to the surface of the cover 330. When the cover 330 is turned to cover the fixed base 310, the locking part 332 is locked with the fixed base 310. In some embodiments, a threaded hole is formed in the locking part 332, and a through hole is formed in the fixed base 310 to expose the locking part 332 to the outside of the fixed base 310. A screw is arranged outside the fixed base 310 to pass through the through hole of the fixed base 310 and lock with the locking part 332. In this way, the locking part 332 is fixedly connected with the fixed base 310, and the cover 330 cannot be opened any more, which meets the use requirement.

[0031] In some embodiments, the cover 330 is locked with the fixed base 310 when the kit is not used any more. In some embodiments, the other end of the cover 330 is provided with a locking part 332 which is perpendicular to the surface of the cover 330. When the cover 330 is turned to cover the fixed base 310, the locking part 332 is locked with the fixed base 310. In some embodiments, a threaded hole is formed in the locking part 332, and a through hole is formed in the fixed base 310 to expose the locking part 332 to the outside of the fixed base 310. A screw is arranged outside the fixed base 310 to pass through the through hole of the fixed base 310 and lock with the locking part 332. In this way, the locking part 332 is fixedly connected with the fixed base 310, and the cover 330 cannot be opened any more, which meets the use requirement.

[0032] In some embodiments, the first rocker 210 and the second rocker 320 are arranged in the same plane relative to the connecting rod 220. In this way, the power output by the output shaft 110 is transmitted in the same plane, and the opening and closing device 10 is not unstable. In addition, the power output by the output shaft 110 does not have unnecessary component force, and the working efficiency of the opening and closing device is not reduced. Furthermore, the length of the first rocker 210 is equal to the length of the second rocker 320, and the length of the first rocker 210 is less than the length of the connecting rod 220. In this way, the working state of the power mechanism 100 and the action mechanism 300 can meet the requirement of opening and closing.

[0033] In some embodiments, the first rocker 210 is rotatably connected with the connecting rod 220 through the rotating shaft 230, the rotating shaft 230 comprises a first shaft section 231 and a second shaft section 232, the first shaft section 231 and the second shaft section 232 have different diameters, the first rocker 210 is provided with a first through hole 211 matched with the first shaft section 231, and the connecting rod 220 is provided with a second through hole 221 matched with the second shaft section 232. The first through hole 211 has a hole diameter matched with the first shaft section 231, the second through hole 221 has a hole diameter matched with the second shaft section 232, that is, the hole diameters of the first through hole 211 and the second through hole 221 are also different. The first through hole 211 is provided with an internal thread (not shown in the figure), the first shaft section 231 is provided with a thread, the rotating shaft 230 is provided with an external thread matched with the internal thread, and the rotating shaft 230 is fixedly connected with the first rocker 210 through the thread, the second shaft section 232 is a light shaft, that is, the second shaft section 232 is not provided with a thread, so that after the rotating shaft 230 is fixedly connected with the first rocker 210, the connecting rod 220 is rotatably connected with the first rocker 210 through the light shaft of the second shaft section 232, and is not locked, and the diameter of the second shaft section 232 is greater than that of the first shaft section 231. In addition, the rotating shaft 230 is further provided with a third shaft section 233 at one end of the second shaft section 232 away from the first shaft section 231, and the diameter of the third shaft section 233 is greater than that of the second shaft section 232, so as to avoid the disconnection of the connecting rod 220 and the second shaft section 232.

[0034] Similarly, the second rocker 320 is rotatably connected with the connecting rod 220 through the rotating shaft 240, that is, the rotating shaft 240 is similar to the rotating shaft 230, the second rocker 320 is fixedly connected with the rotating shaft 240, and the connecting rod 220 is rotatably connected with the second rocker 320 through the rotating shaft 240.

[0035] The embodiment of the present application also provides a real-time fluorescent PCR analyzer, which comprises the switch cover device 10 in any of the above embodiments. The real-time fluorescent PCR (Polymerase Chain Reaction) analyzer is an instrument for adding a fluorescent gene in a PCR reaction system, monitoring the whole PCR process in real time by using a fluorescent signal accumulation, and finally quantitatively analyzing an unknown template through a standard curve. Figure 1 and Figure 2As shown, the analyzer has a mounting seat 400, and one or more mounting holes 410 are formed in the mounting seat 400, and the switch cover device 10 is fixedly connected with the analyzer through the mounting holes 410. In some embodiments, the mounting seat 400 and the fixing seat 310 in the switch cover device 10 can be part of the shell of the analyzer. By arranging the switch cover device 10 in the analyzer, the analyzer can be conveniently opened or closed, the overall structure is simple and exquisite, and the realization is easy. Moreover, by automatically controlling the power mechanism 100 through the signal, the opening and closing of the multiple instrument channels can be easily realized, the control is accurate, and the detection efficiency is greatly improved.

[0036] In summary, the switch cover device 10 and the real-time fluorescent PCR analyzer provided by the embodiments of the present application have a simple and exquisite overall structure, are easy to realize, and can be conveniently applied to small detection instruments. Moreover, by automatically controlling the power mechanism 100 through the signal, the opening and closing of the multiple instrument channels can be easily realized, the control is accurate, and the detection efficiency is greatly improved.

[0037] The above merely describes the preferred embodiments of the present application, but the protection scope of the present application is not limited thereto, and any person skilled in the art can easily think of changes or replacements within the technical range disclosed by the present application, which should be covered within the protection scope of the present application.

Claims

1. A real-time fluorescence PCR analyzer, characterized in that, Includes a cover opening and closing device, the cover opening and closing device comprising: The power mechanism includes the output shaft; The transmission mechanism includes a first rocker arm and a connecting rod, one end of the first rocker arm is rotatably connected to the output shaft, and the other end of the first rocker arm is rotatably connected to one end of the connecting rod. The actuating mechanism includes a fixed base, a second rocker arm, and a cover. The cover is hinged to the fixed base, and a limiting hole is provided on the fixed base. One end of the second rocker arm is fixedly connected to the hinged end of the cover, and the other end of the second rocker arm passes through the limiting hole and is rotatably connected to the other end of the connecting rod, so that the second rocker arm can drive the cover to rotate relative to the fixed base to open and close the cover. The fixed base has a receiving cavity, and a ring of engaging parts connected end to end is provided on the surface of the cover facing the fixed base. When the cover rotates to close with the fixed base, the engaging parts are received in the receiving cavity. The surface of the cover facing the fixed seat also has a locking part protruding out. The locking part is perpendicular to the surface of the cover. When the cover is rotated to close with the fixed seat, the locking part locks with the fixed seat. The mounting base has one or more mounting holes, through which the switch cover device is fixedly connected to the analyzer.

2. The real-time fluorescence PCR analyzer according to claim 1, characterized in that, The first rocker arm rotates relative to the connecting rod and the second rocker arm rotates relative to the connecting rod in the same plane.

3. The real-time fluorescence PCR analyzer according to claim 2, characterized in that, The length of the first rocker arm is equal to the length of the second rocker arm, and the length of the first rocker arm is less than the length of the connecting rod.

4. The real-time fluorescence PCR analyzer according to claim 1, characterized in that, The first rocker arm and the connecting rod are rotatably connected by a rotating shaft. The rotating shaft includes a first shaft segment and a second shaft segment. The diameters of the first shaft segment and the second shaft segment are not equal. The first rocker arm has a first through hole that matches the first shaft segment, and the connecting rod has a second through hole that matches the second shaft segment.

5. The real-time fluorescence PCR analyzer according to claim 4, characterized in that, The diameter of the second shaft segment is larger than the diameter of the first shaft segment.

6. The real-time fluorescence PCR analyzer according to claim 5, characterized in that, The first through hole is provided with an internal thread, and the first shaft segment is provided with an external thread that matches the internal thread.

7. The real-time fluorescence PCR analyzer according to claim 5, characterized in that, The rotating shaft also has a third shaft segment at the end of the second shaft segment away from the first shaft segment. The diameter of the third shaft segment is larger than the diameter of the second shaft segment to prevent the connecting rod from disengaging from the second shaft segment.

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