A method for breeding chicken breeding lines
By measuring and analyzing the combining ability and performance of the parent generation of Kebai chickens, high-quality commercial generations were selected. By combining cage rearing and free-range rearing methods, the breeding process of breeding chickens was optimized, which solved the problems of low breeding efficiency and low survival rate of breeding chicken breeding lines, and achieved efficient breeding chicken selection and high-performance commercial generation.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- JIANGMEN KELANG AGRI TECH CO LTD
- Filing Date
- 2022-02-28
- Publication Date
- 2026-05-26
AI Technical Summary
Existing breeding methods for breeding chickens are inefficient, have low survival rates, consume a large number of samples for research, and excessive human intervention can lead to inaccurate breeding results, making it impossible to effectively evaluate the reproductive performance of breeding chickens.
By determining the parent-generation combining ability test scheme of Kebai chickens, commercial generation was selected, and its performance was measured and analyzed in detail. High-quality grandparent breeder chickens were selected for breeding. A combination of cage rearing and free-range rearing was adopted. Multiple data were recorded and statistically analyzed to evaluate the growth, survival, egg production and other performance of the flock. Finally, the combination of BM1 and BN1 was selected as the final breeding line.
It significantly improved the performance of commercial chickens, with a weight of 2100 grams at 56 days and a feed conversion ratio of 1.88:1. Commercial broilers were marketed 3 days earlier than traditional ones, increasing breeding profits. Furthermore, the use of gold and silver feathers to distinguish between males and females saved identification costs and avoided damage to chicks.
Abstract
Description
Technical Field
[0001] This invention belongs to the field of breeding technology for breeding chicken lines, and specifically relates to a method for breeding chicken lines. Background Technology
[0002] A breeding system refers to a system that uses two or more specialized strains (including paternal and maternal lines) as parents. Through rigorously designed hybridization experiments, one combination is selected as the "optimal" hybridization model, and then crossbreeding is performed using this model to produce the next generation of chicks. Breeding chickens are specialized chickens used specifically to produce the next generation of chicks. For chicks to hatch, they must have fertilized eggs, which are laid by breeding chickens, including roosters and hens. Breeding chickens are required to have good genetics, be healthy and free from infectious diseases, and have good breeding performance, among many other characteristics. Selecting a good breeding chicken requires at least four rounds of selection. Eggs must be fertilized to become fertilized eggs, and only fertilized eggs can hatch into new chicks. Eggs laid by hens alone cannot hatch. Therefore, the purpose of breeding chickens is to produce the next generation of organisms.
[0003] The Xiaobai chicken, also known as the broiler hybrid chicken or 817, is the offspring of a cross between fast-growing broiler chickens (such as the AB line of Cobb, AA+, and Ross 308) and high-performing commercial laying hens (such as Lohmann and Hy-Line). Its hatching cost is low, and the commercial broilers grow quickly with good feed conversion ratios. However, the problem lies in the lack of standardized breeding practices in most Xiaobai chicken production. The breeding stock primarily uses commercial laying hens raised by farmers as the female line, the origin of the roosters is unclear, and disease eradication efforts are inadequate. This results in poor uniformity, poor disease resistance, and frequent disease outbreaks in the commercial offspring. Therefore, this paper proposes breeding techniques and methods for chicken breeding systems. Summary of the Invention
[0004] The technical problem to be solved by the present invention is to overcome the existing defects and provide a breeding method for breeding chickens, so as to solve the problems mentioned in the background art, such as low breeding efficiency and low survival rate under natural conditions, large consumption of samples available for research, which is not conducive to later breeding, and excessive human intervention will lead to different and inaccurate breeding results, making it impossible to evaluate the reproduction of breeding chickens.
[0005] To achieve the above objectives, the present invention provides the following technical solution: a method for breeding chicken breeding lines, comprising the following steps:
[0006] Step 1: Determine the testing scheme for the parent-generation combining ability of Kebai chickens;
[0007] Step 2: Analyze the parental generation's combining ability test data to select commercial offspring;
[0008] Step 3: Determine the compatibility of the product;
[0009] Step 4: Product data analysis;
[0010] Step 5: Conduct meat quality testing on commercial broiler chickens;
[0011] Step 6: Analyze the production performance of the commercial generation and its parent generations according to Step 3;
[0012] Step 7: Select grandparent breeder chickens to breed commercial offspring.
[0013] Preferably, step 1 includes the following steps:
[0014] Step 1.1: Select healthy Kebai C strain roosters M and N with similar average weight and test their combining ability with 3 different breeds of laying hens; at hatching, select healthy female chicks, 1500 each, and label them as M1, M2, M3, N1, N2, and N3, with 3 replicates in each group, and ensure that the feeding conditions of the 6 groups are consistent during the feeding process;
[0015] Step 1.2: Record feed intake and mortality, calculate the average daily feed intake per bird per week, and calculate the survival rate for birds aged 1-20 weeks and during the laying period.
[0016] Step 1.3: Measure weight gain weekly from birth, and conduct random body size measurements at specific age weeks;
[0017] Step 1.4: After the start of laying, record the number of eggs laid each day, sample the egg weight each week, and collect data such as the age at the start of laying, weekly laying rate, average egg weight, average daily feed intake, and feed conversion ratio. The feeding cycle is 66 weeks.
[0018] Preferably, step 2 includes the following steps:
[0019] Step 2.1: Survival rate analysis. The results showed that the survival rate of all six combinations during the rearing period was above 96%. There were differences in the survival rate of the five combinations during the laying period. Combination M1 had the highest survival rate at 96.2%, followed by combination N1, while combination N3 had the lowest survival rate at only 93.8%.
[0020] Step 2.2: Growth rate analysis. By statistically analyzing the body weight of parent hens in each testing period, it can be seen that combination M1 had the best weight gain, followed by combination N1, and combination 5 had the worst. Combination 5 had the smallest coefficient of variation at each age, while combination 3 had the largest. Combination M was a normal-legged combination, while combination N was a dwarf combination.
[0021] Step 2.3: Egg production performance. Statistical analysis of egg production performance of different parent generation combinations shows that combination N3 had the earliest age of onset of egg production, the highest number of eggs produced, and the largest egg weight. In summary, combination N3 had the best egg production performance, followed by combination M1, then combination N2, and combination M2 had the worst.
[0022] Step 2.4: Feed conversion ratio analysis during the laying period. The experimental results showed that the feed conversion ratio during the laying period of combination N1 was the lowest, at only 2.20:1, while the feed conversion ratios of combinations M1, M2, M3, N2, and N3 were 2.25, 2.32, 2.38, 2.28, and 2.26:1, respectively.
[0023] Step 2.5: Based on comprehensive performance analysis, select combination M1 and combination N1 for product substitution compatibility testing.
[0024] Preferably, step 3 includes the following steps:
[0025] Step 3.1: Select paternal roosters from three preserved and bred strains and test their combining ability with two selected parent strains;
[0026] Step 3.2: Select 1200 healthy male and 1200 female seedlings (600 males and 600 females) at the time of emergence, and label them as AM1, AN1, BM1, BN1, CM1 and CN1, with 3 replicates per group;
[0027] Step 3.3: Divide the number of each group into two equal parts. One part is raised in cages, and the other part is raised on the ground. During the rearing process, ensure that the rearing conditions of the six groups are the same.
[0028] Step 3.4: Record daily feed intake and mortality; calculate the average daily feed intake per bird per week, and weigh 100 birds from each group each week. Weigh the entire group at the end of the experiment.
[0029] Step 3.5: At 56 days of age, 10 chickens from each replicate group (5 males and 5 females, for a total of 180 chickens) were selected for a slaughter experiment;
[0030] Step 3.6: Calculate the hatching weight, body weight at 28 days, 42 days, 49 days, and 56 days of age, feed conversion ratio, survival rate, slaughter rate, leg muscle percentage, breast muscle percentage, and abdominal fat percentage for each combination of male and female chickens.
[0031] Preferably, step 4 includes the following steps:
[0032] Step 4.1: Survival rate analysis. The experimental results showed that the survival rate of four combinations was above 96%, with combinations BM1 and BN1 exceeding 98%; the survival rates of combinations AM1 and AN1 were only 95.2% and 95.6%, respectively.
[0033] Step 4.2: Growth rate analysis. The body weight of each combination of commercial generation was recorded. It can be seen that the body weight of combination B is the largest, followed by combination C; and the body weight of parent combination N1 is also larger than that of parent combination M1.
[0034] Step 4.3: Feed conversion rate analysis. The feed conversion rates of each combination in the commercial generation experiment were recorded. It can be seen that the feed conversion rate of combination C was the lowest, followed by combination B, and the difference between them was not significant. The feed conversion rate of parent combination N1 was also lower than that of parent combination M1.
[0035] Step 4.4: Slaughter performance analysis. After recording the slaughter performance of each combination in the commercial trial, it can be seen that the slaughter rate, semi-eviscerated rate and fully eviscerated rate of the C rooster combination are the highest, while the difference between the C combination and the C combination is not significant. The breast muscle rate of the C combination is lower, but the leg muscle rate is the highest, which meets the consumption requirements of the white chicken.
[0036] Step 4.5: Based on the above performance analysis, the BM1 combination becomes the final matching system for Kebai No. 1, and the BN1 combination becomes the final matching system for Kebai No. 2.
[0037] Preferably, step 5 specifically includes selecting 30 commercial broiler chickens (15 males and 15 females) for Kebai No. 1 to conduct meat quality testing. According to the test results, Kebai No. 1 has good meat tenderness; it also has high water holding capacity and low cooking loss rate, which is conducive to improving the yield during product processing; and it has high intramuscular fat content, which is conducive to maintaining the taste and flavor of the muscle.
[0038] Preferably, step 7 includes the following steps:
[0039] Step 7.1: Select 50 hens from the parent generation that are growing and developing normally, and select 10 roosters from the grandparent generation. It is important to select healthy roosters that are at the right weight, well-developed, with soft abdomens, and exhibit sexual reflexes such as anal eversion and erection of the mating organ when massaged. Combine this with training to collect semen and check the quality of the semen.
[0040] Step 7.2: Separate the roosters and hens and raise them separately for two consecutive weeks;
[0041] Step 7.3: Test the health of roosters and hens separately, and vaccinate those that need to be vaccinated;
[0042] Step 7.4: After 2 weeks, select healthy roosters and hens as breeding stock, ensuring that there are 5-10 roosters and 30-40 hens.
[0043] The preferred step 7 further includes the following steps:
[0044] Step 7.5: Isolation and training. Roosters should be moved to individual cages 3-4 weeks before use to facilitate familiarization with the environment and management personnel.
[0045] Step 7.6: Collect semen once a day or every other day. Once training is successful, continue collecting semen every other day. After 3-4 training sessions, most roosters will be able to produce semen. Some well-developed roosters, with proficient semen collection techniques, can produce semen on the first day of training. However, some roosters, despite repeated training, still cannot establish a conditioned reflex. If such roosters have not reached sexual maturity, training should continue and feeding management should be strengthened. Otherwise, they should be culled. Under normal circumstances, about 3% to 5% of such roosters should be culled.
[0046] Step 7.7: To prevent semen contamination, before training the rooster, trim about 1 cm of feathers around the cloaca. On the day of semen collection, the rooster must fast for 3-4 hours before collection to prevent defecation and urination. All artificial insemination equipment, including at least 5 tubes and 2 insemination droppers, should be cleaned, disinfected, and dried. If drying equipment is unavailable, after cleaning, sterilize by boiling in distilled water, and then rinse 2-3 times with physiological saline before use.
[0047] Step 7.8: During the insemination procedure, the hens to be inseminated must first undergo a white pullorum test. Any hens that test positive must be culled. Ideally, the hens should be selected without cloacal inflammation, in moderate nutritional condition, and have an egg production rate of 50%.
[0048] Step 7.9: Two people are needed for insemination. The assistant holds the hen's wings with his left hand and lifts it up so that the hen's head is facing upwards and the vent is facing downwards. The assistant places his right palm under the hen's pubis and applies a certain amount of pressure to the soft part of the abdomen. The oviduct opening in the cloaca will then turn outwards, and the inseminator can then gently insert the insemination device into the center of the insemination tube opening.
[0049] Step 7.10: Artificial insemination of caged hens does not require removing the hen from the cage. The assistant simply holds the hen's legs with their left hand, lifts it slightly, and places the hen's chest against the cage door. The right hand applies pressure to the abdomen, exposing the oviduct opening, allowing the inseminator to inject semen.
[0050] Preferably, step 7 further includes the following steps:
[0051] Step 7.11: Conduct health checks on the commercial stock during the brooding period;
[0052] Step 7.12: After the brooding period, divide the chicks into two groups: free-range and pen-raised, and count their numbers. The pen-raised chicks should be divided into at least three groups.
[0053] Step 7.13: Statistical analysis of the survival rate and morbidity rate of the flock at 20-23 weeks of age;
[0054] Step 7.14: Collect statistics on feed consumption, body weight, and egg production of the flock during the 20-30 week period;
[0055] Step 7.15: Assess the growth conditions of the chicken flocks based on their feed consumption, body weight, and egg production.
[0056] Compared with the prior art, the present invention provides a method for breeding chicken breeding lines, which has the following beneficial effects:
[0057] 1. This invention screens out the corresponding commercial generation by measuring the combining ability of the parent generation of Kebai chickens, and measures the performance of the commercial generation. The analysis results show that the egg production of 66-week-old breeding chickens is 5-8 fewer than that of high-yielding commercial laying hens, while the performance of the commercial generation is more than 10% higher than that of "817". The breast and leg muscles are basically similar to those of "817", and the meat quality is also significantly better. The breeding benefits are significant, and it has good market promotion prospects.
[0058] 2. This invention, through the selection and breeding of the first paternal parent of white chicks, cultivates a high-yield strain with high egg production in the parent generation and a breeding cost that is basically similar to that of high-yield laying hens. The chicks reach a weight of 2100 grams at 56 days, with a feed conversion ratio of only 1.88:1. The commercial broilers are marketed 3 days earlier than the traditional "817" strain, which can significantly improve the profit of commercial broiler breeding. At the same time, the sex of the parent generation can be distinguished by gold and silver feathers, and the sex of the commercial generation can be distinguished by fast and slow feathers, which saves identification costs and avoids damage to the chicks caused by venting. Detailed Implementation
[0059] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.
[0060] This invention provides a technical solution: a method for breeding chicken mating lines, comprising the following steps:
[0061] Step 1: Determine the testing scheme for the parent-generation combining ability of Kebai chickens;
[0062] Step 2: Analyze the parental generation's combining ability test data to select commercial offspring;
[0063] Step 3: Determine the compatibility of the product;
[0064] Step 4: Product data analysis;
[0065] Step 5: Conduct meat quality testing on commercial broiler chickens;
[0066] Step 6: Analyze the production performance of the commercial generation and its parent generations according to Step 3; the analysis of the parent generation's production performance yields the following results:
[0067] (1) The survival rate during the brooding and rearing period was 97%, and the survival rate during the egg-laying period was 94%;
[0068] (2) The peak egg production rate is above 90%, the number of hatching eggs produced at 66 weeks of age is 282, and the egg weight at 25 weeks of age is 48.4g;
[0069] (3) Weight at the start of labor: 1700g;
[0070] (4) Feed conversion ratio during the laying period: 2.25:1;
[0071] (5) Parents can distinguish males and females by their own gold or silver feathers;
[0072] Analysis of the production performance of the commodity generation shows that:
[0073] (1) The survival rate during the brooding and rearing period was 98%;
[0074] (2) The uniformity of the slaughtered chicken flock is above 90%;
[0075] (3) Chickens are marketed at 49-51 days old, with a slaughter weight of 1.65-1.75 kg for both male and female chickens.
[0076] (4) Feed conversion ratio 1.85-1.90:1;
[0077] (5) Broilers have yellow skin, yellow shanks, and well-developed combs.
[0078] (6) The muscle has strong water retention, tender texture and good flavor.
[0079] (7) The product can distinguish the sex of the male and female by the speed of feathers.
[0080] Step 7: Select grandparent breeder chickens to breed commercial offspring.
[0081] In this invention, preferably, step 1 includes the following steps:
[0082] Step 1.1: Select healthy Kebai C strain roosters M and N with similar average weight and test their combining ability with 3 different breeds of laying hens; at hatching, select healthy female chicks, 1500 each, and label them as M1, M2, M3, N1, N2, and N3, with 3 replicates in each group, and ensure that the feeding conditions of the 6 groups are consistent during the feeding process;
[0083] Step 1.2: Record feed intake and mortality, calculate the average daily feed intake per bird per week, and calculate the survival rate for birds aged 1-20 weeks and during the laying period.
[0084] Step 1.3: Measure weight gain weekly from birth, and conduct random body size measurements at specific age weeks;
[0085] Step 1.4: After the start of laying, record the number of eggs laid each day, sample the egg weight each week, and collect data such as the age at the start of laying, weekly laying rate, average egg weight, average daily feed intake, and feed conversion ratio. The feeding cycle is 66 weeks.
[0086] In this invention, preferably, step 2 includes the following steps:
[0087] Step 2.1: Survival rate analysis. The results showed that the survival rate of all six combinations during the rearing period was above 96%. There were differences in the survival rate of the five combinations during the laying period. Combination M1 had the highest survival rate at 96.2%, followed by combination N1, while combination N3 had the lowest survival rate at only 93.8%.
[0088] combination M1 M2 M3 N1 N2 N3 Newborn 37.8±2.0 37.5±2.6 37.8±2.4 38.4±2.7 36.8±2.5 37.0±2.5 Weight at the start of labor 1702±115 1623±108 1585±115 1532±110 1514±110 1464±119 Weight at 43 weeks 2185±152 2145±145 2122±173 2038±155 1988±142 2005±152 Weight at 66 weeks 2210±163 2188±155 2160±178 2052±156 2013±148 2023±158
[0089] Table 1
[0090] combination M1 M2 M3 N1 N2 N3 Age at onset of labor 138 136 138 139 139 133 Egg weight at 25 weeks / g 48.4±2.3 46.8±2.4 47.5±2.8 49.1±2.6 48.0±2.0 47.6±2.8 Egg production at 43 weeks 135±9 125±10 130±11 132±9 128±9 139±10 Egg production at 66 weeks 282±25 269±28 276±25 280±22 272±26 286±26
[0091] Table 2
[0092] Step 2.4: Feed conversion ratio analysis during the laying period. The experimental results showed that the feed conversion ratio during the laying period of combination N1 was the lowest, at only 2.20:1, while the feed conversion ratios of combinations M1, M2, M3, N2, and N3 were 2.25, 2.32, 2.38, 2.28, and 2.26:1, respectively.
[0093] Step 2.5: Based on comprehensive performance analysis, select combination M1 and combination N1 for product substitution compatibility testing.
[0094] In this invention, preferably, step 3 includes the following steps:
[0095] Step 3.1: Select paternal roosters from three preserved and bred strains and test their combining ability with two selected parent strains;
[0096] Step 3.2: Select 1200 healthy male and 1200 female seedlings (600 males and 600 females) at the time of emergence, and label them as AM1, AN1, BM1, BN1, CM1 and CN1, with 3 replicates per group;
[0097] Step 3.3: Divide the number of each group into two equal parts. One part is raised in cages, and the other part is raised on the ground. During the rearing process, ensure that the rearing conditions of the six groups are the same.
[0098] Step 3.4: Record daily feed intake and mortality; calculate the average daily feed intake per bird per week, and weigh 100 birds from each group each week. Weigh the entire group at the end of the experiment.
[0099] Step 3.5: At 56 days of age, 10 chickens from each replicate group (5 males and 5 females, for a total of 180 chickens) were selected for a slaughter experiment;
[0100] Step 3.6: Calculate the hatching weight, body weight at 28 days, 42 days, 49 days, and 56 days of age, feed conversion ratio, survival rate, slaughter rate, leg muscle percentage, breast muscle percentage, and abdominal fat percentage for each combination of male and female chickens.
[0101] In this invention, preferably, step 4 includes the following steps:
[0102] Step 4.1: Survival rate analysis. The experimental results showed that the survival rate of four combinations was above 96%, with combinations BM1 and BN1 exceeding 98%; the survival rates of combinations AM1 and AN1 were only 95.2% and 95.6%, respectively.
[0103] Age in days AM1 AN1 BM1 BN1 CM1 CN1 7 117±8.3 121±8.8 130±9.2 128±8.6 129±9.0 128±8.6 28 713±55 702±52 782±53 770±55 748±56 755±50 42 1315±75 1280±72 1372±74 1426±75 1345±76 1360±78 49 1640±135 1618±126 1688±118 1785±120 1652±125 1675±128 56 1910±160 1875±155 2020±154 2128±161 1970±155 2005±151
[0104] Table 3
[0105] Age in days AM1 AN1 BM1 BN1 CM1 CN1 7 0.89 0.86 0.88 0.84 0.87 0.83 28 1.48 1.50 1.48 1.45 1.46 1.46 42 1.69 1.73 1.70 1.68 1.67 1.65 49 1.90 1.90 1.88 1.84 1.84 1.81 56 1.98 2.02 1.92 1.88 1.88 1.86
[0106] Table 4
[0107] unit:% gender AM1 AN1 BM1 BN1 CM1 CN1 Slaughter rate male 89.5±1.3 90.3±1.0 91.0±1.0 90.7±0.8 90.8±0.7 91.2±1.1 mother 88.6±1.1 89.8±1.0 90.6±0.8 90.4±0.9 90.6±0.6 90.8±0.8 Semi-cleaned rate male 84.2±1.0 85.1±1.2 86.4±0.9 86.0±1.0 86.2±0.8 86.5±1.1 mother 83.5±0.9 84.6±1.3 85.8±0.9 85.5±0.8 85.6±0.8 86.0±0.8 Full turret clearance male 70.8±0.8 71.6±1.2 72.8±0.6 72.6±0.7 72.4±0.5 73.0±0.8 mother 71.1±0.9 71.7±1.3 73.2±0.8 72.8±0.8 72.9±0.7 73.3±1.0 pectoral muscle rate male 16.2±0.6 16.4±0.8 15.8±0.6 16.1±0.6 15.8±0.5 16.0±0.6 mother 17.2±0.6 17.5±0.7 16.8±0.5 16.6±0.6 16.8±0.7 17.0±0.8 Leg muscle rate male 22.9±1.2 23.2±1.4 23.8±1.0 24.0±1.2 23.6±0.9 23.8±1.0 mother 22.4±0.8 22.4±1.0 23.1±0.6 23.2±0.8 23.2±0.9 22.7±1.1
[0108] Table 5
[0109] Step 4.5: Based on the above performance analysis, the BM1 combination becomes the final matching system for Kebai No. 1, and the BN1 combination becomes the final matching system for Kebai No. 2.
[0110] index Rooster hen tenderness 1.96±0.43 1.82±0.36 Water system capacity (%) 97.52±1.26 96.93±1.35 Cooking loss (%) 19.65±3.68 19.32±3.23 Intramuscular fat (%) 2.28±0.75 2.40±0.66 Crude protein (%) 23.87±1.46 23.45±2.12
[0111] Table 6
[0112] In this invention, preferably, step 7 includes the following steps:
[0113] Step 7.1: Select 50 hens from the parent generation that are growing and developing normally, and select 10 roosters from the grandparent generation. It is important to select healthy roosters that are at the right weight, well-developed, with soft abdomens, and exhibit sexual reflexes such as anal eversion and erection of the mating organ when massaged. Combine this with training to collect semen and check the quality of the semen.
[0114] Step 7.2: Separate the roosters and hens and raise them separately for two consecutive weeks;
[0115] Step 7.3: Test the health of roosters and hens separately, and vaccinate those that need to be vaccinated;
[0116] Step 7.4: After 2 weeks, select healthy roosters and hens as breeding stock, ensuring that there are 5-10 roosters and 30-40 hens.
[0117] In this invention, preferably, step 7 further includes the following steps:
[0118] Step 7.5: Isolation and training. Roosters should be moved to individual cages 3-4 weeks before use to facilitate familiarization with the environment and management personnel.
[0119] Step 7.6: Collect semen once a day or every other day. Once training is successful, continue collecting semen every other day. After 3-4 training sessions, most roosters will be able to produce semen. Some well-developed roosters, with proficient semen collection techniques, can produce semen on the first day of training. However, some roosters, despite repeated training, still cannot establish a conditioned reflex. If such roosters have not reached sexual maturity, training should continue and feeding management should be strengthened. Otherwise, they should be culled. Under normal circumstances, about 3% to 5% of such roosters should be culled.
[0120] Step 7.7: To prevent semen contamination, before training the rooster, trim about 1 cm of feathers around the cloaca. On the day of semen collection, the rooster must fast for 3-4 hours before collection to prevent defecation and urination. All artificial insemination equipment, including at least 5 tubes and 2 insemination droppers, should be cleaned, disinfected, and dried. If drying equipment is unavailable, after cleaning, sterilize by boiling in distilled water, and then rinse 2-3 times with physiological saline before use.
[0121] Step 7.8: During the insemination procedure, the hens to be inseminated must first undergo a white pullorum test. Any hens that test positive must be culled. Ideally, the hens should be selected without cloacal inflammation, in moderate nutritional condition, and have an egg production rate of 50%.
[0122] Step 7.9: Two people are needed for insemination. The assistant holds the hen's wings with his left hand and lifts it up so that the hen's head is facing upwards and the vent is facing downwards. The assistant places his right palm under the hen's pubis and applies a certain amount of pressure to the soft part of the abdomen. The oviduct opening in the cloaca will then turn outwards, and the inseminator can then gently insert the insemination device into the center of the insemination tube opening.
[0123] Step 7.10: Artificial insemination of caged hens does not require removing the hen from the cage. The assistant simply holds the hen's legs with their left hand, lifts it slightly, and places the hen's chest against the cage door. The right hand applies pressure to the abdomen, exposing the oviduct opening, allowing the inseminator to inject semen.
[0124] In this invention, preferably, step 7 further includes the following steps:
[0125] Step 7.11: Conduct health checks on the commercial stock during the brooding period;
[0126] Step 7.12: After the brooding period, divide the chicks into two groups: free-range and pen-raised, and count their numbers. The pen-raised chicks should be divided into at least three groups.
[0127] Step 7.13: Statistical analysis of the survival rate and morbidity rate of the flock at 20-23 weeks of age;
[0128] Step 7.14: Collect statistics on feed consumption, body weight, and egg production of the flock during the 20-30 week period;
[0129] Step 7.15: Assess the growth conditions of the chicken flocks based on their feed consumption, body weight, and egg production.
[0130] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.
Claims
1. A method for breeding a breeding chicken pair, characterized in that: Includes the following steps: Step 1: Determine the testing scheme for the parent-generation combining ability of Kebai chickens; Step 2: Analyze the parental generation's combining ability test data to select commercial offspring; Step 3: Determine the compatibility of the product; Step 4: Product data analysis; Step 5: Conduct meat quality testing on commercial broiler chickens; Step 6: Analyze the production performance of the commercial generation and its parent generations according to Step 3; Step 7: Select grandparent breeder chickens to breed commercial offspring; Step 1 includes the following steps: Step 1.1: Select healthy Kebai C strain roosters M and N with similar average weight and test their combining ability with 3 different breeds of laying hens; at hatching, select healthy female chicks, 1500 each, and label them as M1, M2, M3, N1, N2, and N3, with 3 replicates in each group, and ensure that the feeding conditions of the 6 groups are consistent during the feeding process; Step 1.2: Record feed intake and mortality, calculate the average daily feed intake per bird per week, and calculate the survival rate for birds aged 1-20 weeks and during the laying period. Step 1.3: Measure weight gain weekly from birth, and conduct random body size measurements at specific age weeks; Step 1.4: After the start of laying, record the daily number of eggs laid, sample the egg weight weekly, and collect data on the age at the start of laying, weekly laying rate, average egg weight, average daily feed intake, and feed conversion ratio. The feeding cycle is 66 weeks. Step 2 includes the following steps: Step 2.1: Survival rate analysis. The results showed that the survival rate of all six combinations during the rearing period was above 96%. There were differences in the survival rate of the five combinations during the laying period. Combination M1 had the highest survival rate at 96.2%, followed by combination N1, while combination N3 had the lowest survival rate at only 93.8%. Step 2.2: Growth rate analysis. Statistical analysis of the body weight of parent hens in each testing period showed that combination M1 had the best weight gain, followed by combination N1, and combination 5 had the worst. Combination 5 had the smallest coefficient of variation at each age, while combination 3 had the largest. Combination M was a normal-legged combination, while combination N was a dwarf combination. Step 2.3: Egg production performance analysis. Statistical analysis of egg production in different parent generation combinations showed that combination N3 had the earliest age at onset of egg production, the highest number of eggs produced, and the largest egg weight. Overall, combination N3 had the best egg production performance, followed by combination M1, then combination N2, and combination M2 had the worst. Step 2.4: Feed conversion ratio analysis during the laying period. The experimental results showed that the feed conversion ratio during the laying period of combination N1 was the lowest, at only 2.20:1, while the feed conversion ratios of combinations M1, M2, M3, N2, and N3 were 2.25, 2.32, 2.38, 2.28, and 2.26:1, respectively. Step 2.5: Based on comprehensive performance analysis, select combination M1 and combination N1 for product substitution compatibility testing; Step 3 includes the following steps: Step 3.1: Select paternal roosters from three preserved and bred strains and test their combining ability with two selected parent strains; Step 3.2: Select 1200 healthy male and 1200 female seedlings at the time of emergence, 600 males and 600 females, and label them as AM1, AN1, BM1, BN1, CM1 and CN1, with 3 replicates per group; Step 3.3: Divide the number of each group into two equal parts. One part is raised in cages, and the other part is raised on the ground. During the rearing process, ensure that the rearing conditions of the 6 groups are the same. Step 3.4: Record daily feed intake and mortality; calculate the average daily feed intake per bird per week, and weigh 100 birds from each group each week. Weigh the entire group at the end of the experiment. Step 3.5: At 56 days of age, 10 chickens from each replicate group were selected, including 5 males and 5 females, for a total of 180 chickens, for slaughter experiment; Step 3.6: Calculate the hatching weight, body weight at 28 days, 42 days, 49 days, and 56 days, feed conversion ratio, survival rate, dressing percentage, leg muscle percentage, breast muscle percentage, and abdominal fat percentage for each combination of male and female chickens. Step 4 includes the following steps: Step 4.1: Survival rate analysis. The experimental results showed that the survival rate of four combinations was above 96%, with combinations BM1 and BN1 exceeding 98%; the survival rates of combinations AM1 and AN1 were only 95.2% and 95.6%, respectively. Step 4.2: Growth rate analysis. The body weight of each combination of commercial generation was recorded. It was found that the body weight of combination B was the largest, followed by combination C; and the body weight of parent combination N1 was also larger than that of parent combination M1. Step 4.3: Feed conversion rate analysis. The feed conversion rates of each combination in the commercial generation experiment were recorded. It was found that the feed conversion rate of combination C was the lowest, followed by combination B, and the difference between them was not significant. The feed conversion rate of parent combination N1 was also lower than that of parent combination M1. Step 4.4: Slaughter performance analysis. After recording the slaughter performance of each combination in the commercial trial, it was found that the slaughter rate, semi-eviscerated rate and fully eviscerated rate of the C rooster combination were the highest, while the difference between the C and B combinations was not significant. The breast muscle rate of the B combination was lower, but the leg muscle rate was the highest, which met the consumption requirements of the white chicken. Step 4.5: Based on the above performance analysis, the BM1 combination becomes the final matching system for Kebai No. 1, and the BN1 combination becomes the final matching system for Kebai No.
2.
2. The method for breeding a breeding chicken pair according to claim 1, characterized in that: Step 5 specifically involves selecting 30 commercial broiler chickens (15 males and 15 females) for the Kebai No. 1 breed and conducting meat quality testing. The test results show that Kebai No. 1 has good tenderness, high water holding capacity, and low cooking loss rate, which is beneficial to improving the yield during product processing. It also has high intramuscular fat content, which is beneficial to maintaining the texture and flavor of the muscle.
3. The method for breeding a breeding chicken pair according to claim 1, characterized in that: Step 7 includes the following steps: Step 7.1: Select 50 hens from the parent generation that are growing and developing normally, and select 10 roosters from the grandparent generation. It is important to select healthy roosters that are at the right weight, well-developed, with soft abdomens, and exhibit sexual reflexes such as anal eversion and erection of the mating organ when massaged. Combine this with training to collect semen and check the quality of the semen. Step 7.2: Separate the roosters and hens and raise them separately for two consecutive weeks; Step 7.3: Test the health of roosters and hens separately, and vaccinate those that need to be vaccinated; Step 7.4: After 2 weeks, select healthy roosters and hens as breeding stock, ensuring that there are 5-10 roosters and 30-40 hens.
4. The method for breeding a breeding chicken pair according to claim 3, characterized in that: Step 7 also includes the following steps: Step 7.5: Isolation and training. Roosters should be moved to individual cages 3-4 weeks before use to facilitate familiarization with the environment and management personnel. Step 7.6: Collect semen once a day or every other day. Once training is successful, continue collecting semen every other day. After 3-4 training sessions, most roosters will be able to produce semen. Some well-developed roosters, with proficient semen collection techniques, can produce semen on the first day of training. However, some roosters, despite repeated training, still cannot establish a conditioned reflex. If such roosters have not reached sexual maturity, training should continue and feeding management should be strengthened. Otherwise, they should be culled. Under normal circumstances, 3% to 5% of such roosters should be culled. Step 7.7: To prevent semen contamination, before the rooster begins training, trim about 1 cm of feathers around the cloaca. On the day of semen collection, the rooster must fast for 3-4 hours before collection to prevent defecation and urination. All artificial insemination equipment, including at least 5 tubes and 2 insemination droppers, should be cleaned, disinfected, and dried. If drying equipment is unavailable, after cleaning, sterilize by boiling in distilled water, and then rinse 2-3 times with physiological saline before use. Step 7.8: During the insemination procedure, the hens to be inseminated must first undergo a white pullorum test. Any hens that test positive must be culled. Ideally, the hens should be selected without cloacal inflammation, in moderate nutritional condition, and have an egg production rate of 50%. Step 7.9: Two people are needed for insemination. The assistant holds the hen's wings with his left hand and lifts it up so that the hen's head is facing upwards and the vent is facing downwards. The assistant places his right palm under the hen's pubis and applies a certain amount of pressure to the soft part of the abdomen. The oviduct opening in the cloaca will then turn outwards, and the inseminator can then gently insert the insemination device into the center of the insemination tube opening. Step 7.10: Artificial insemination of caged hens does not require removing the hen from the cage. The assistant simply holds the hen's legs with their left hand, lifts it slightly, and places the hen's chest against the cage door. The right hand applies pressure to the abdomen, exposing the oviduct opening, allowing the inseminator to inject semen.
5. The method for breeding a breeding chicken pair according to claim 3, characterized in that: Step 7 also includes the following steps: Step 7.11: Conduct health checks on the commercial stock during the brooding period; Step 7.12: After the brooding period, divide the chicks into two groups: free-range and pen-raised, and count their numbers. The pen-raised chicks should be divided into at least three groups. Step 7.13: Statistical analysis of the survival rate and morbidity rate of the flock at 20-23 weeks of age; Step 7.14: Collect statistics on feed consumption, body weight, and egg production of the flock during the 20-30 week period; Step 7.15: Assess the growth conditions of the chicken flocks based on their feed consumption, body weight, and egg production.