A sample analysis method, apparatus, and computer readable storage medium

Abstract

The application discloses a sample analysis method, device and computer readable storage medium, wherein the sample analysis method comprises: obtaining a to-be-tested reaction curve of a mixed solution; wherein a to-be-tested sample solution and a reagent solution react to form the mixed solution, and the to-be-tested reaction curve represents a corresponding relationship between an absorbance value of the mixture and time; based on a characteristic parameter of the to-be-tested reaction curve, determining an estimated concentration value of the to-be-tested sample solution in a pre-established calibration curve; based on the estimated concentration value, determining a corresponding true concentration value in a pre-established hook effect curve; and determining a serum concentration of the to-be-tested sample solution according to the true concentration value. In this way, the detection cost can be reduced, and the detection efficiency can be improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of sample detection, in particular to a sample analysis method and device and a computer readable storage medium. BACKGROUND

[0002] The basic principle of traditional immunological serological (agglutination, precipitation, etc.) detection of antigen-antibody is that antigen-antibody molecules move freely and collide with each other in a liquid phase environment to complete their specific binding reaction, and the amount of the binding product is related to the concentration of the reactants. No matter how much antigen is added to a certain amount of antibody or how much antibody is added to a certain amount of antigen, it can be found that the strongest antigen-antibody reaction occurs only when the molecular ratio of the two is appropriate.

[0003] The hook effect generally refers to the fact that in an immunological marker detection method, due to the high content of the detected antigen in the sample, the excess antigen is combined with the solid-phase antibody and the enzyme-labeled antibody, respectively, and no longer forms a "sandwich complex", thereby affecting the detection result and reporting a high concentration as a low concentration, which is called "hook effect", also known as High Dose-HOOK or HD-HOOK. How to avoid the influence of the hook effect on the detection result in the detection has become a key problem. SUMMARY

[0004] To solve the above problems, the present application provides a sample analysis method, device and computer readable storage medium, which can reduce the detection cost and improve the detection efficiency.

[0005] One of the technical solutions adopted by the present application is to provide a sample analysis method, which comprises: obtaining a to-be-detected reaction curve of a mixed solution; wherein the mixed solution is formed by mixing a to-be-detected sample solution and a reagent solution, the to-be-detected sample solution and the reagent solution react to form a mixture, and the reaction curve represents the corresponding relationship between the absorbance value of the mixture and the time; determining an estimated concentration value of the to-be-detected sample solution in a pre-established calibration curve based on a characteristic parameter of the to-be-detected reaction curve; wherein the calibration curve represents the corresponding relationship between the characteristic parameter and the estimated concentration value; determining a corresponding true concentration value in a pre-established hook effect curve based on the estimated concentration value; wherein the hook effect curve represents the corresponding relationship between the estimated concentration value and the true concentration value; and determining the serum concentration of the to-be-detected sample solution according to the true concentration value.

[0006] The method further comprises: obtaining concentration values of a plurality of calibration sample solutions; and obtaining characteristic parameters of reaction curves corresponding to the mixing of the plurality of calibration sample solutions with the reagent solution, respectively; and establishing the calibration curve according to the plurality of calibration concentration values and the corresponding characteristic parameters.

[0007] The method further comprises: obtaining a target reaction curve corresponding to a target sample solution with the maximum concentration value among the plurality of calibration sample solutions; determining an absorbance value corresponding to a first time and an absorbance value corresponding to a second time in the target reaction curve, wherein the second time is greater than the first time.

[0008] The method further comprises: obtaining real concentration values of a plurality of high-concentration sample solutions, wherein the concentration values of the high-concentration sample solutions are greater than the preset concentration value; determining estimated concentration values of the plurality of high-concentration sample solutions according to characteristic parameters of reaction curves of the plurality of high-concentration sample solutions and the calibration curve; and establishing a hook effect curve according to the real concentration values and the estimated concentration values of the plurality of high-concentration sample solutions.

[0009] The hook effect curve is divided into a plurality of concentration intervals based on the real concentration values; and the corresponding real concentration value is determined in the pre-established hook effect model based on the estimated concentration value, including: determining a concentration interval corresponding to the to-be-measured reaction curve; and determining the real concentration value corresponding to the estimated concentration value in the concentration interval corresponding to the to-be-measured reaction curve.

[0010] The hook effect curve is divided into at least a first concentration interval, a second concentration interval and a third concentration interval based on the real concentration values, the demarcation point of the first concentration interval and the second concentration interval is a real concentration value corresponding to a sample solution with the preset concentration value, and the real concentration value corresponding to the maximum characteristic parameter in the reaction curves of the plurality of high-concentration sample solutions is the demarcation point of the second concentration interval and the third concentration interval; wherein the estimated concentration value and the real concentration value in the first concentration interval are equal.

[0011] The determination of the concentration interval corresponding to the to-be-measured reaction curve comprises: when the first time absorbance value of the to-be-measured reaction curve is less than the first time absorbance value of the target reaction curve and the characteristic parameter of the to-be-measured reaction curve is greater than the characteristic parameter of the target reaction curve, the reaction curve corresponds to the first concentration interval; or when the first time absorbance value of the to-be-measured reaction curve is greater than the first time absorbance value of the target reaction curve and the characteristic parameter of the to-be-measured reaction curve is less than the characteristic parameter of the target reaction curve, the reaction curve corresponds to the second concentration interval; or when the first time absorbance value of the to-be-measured reaction curve is less than the first time absorbance value of the target reaction curve and the characteristic parameter of the to-be-measured reaction curve is less than the characteristic parameter of the target reaction curve, the reaction curve corresponds to the third concentration interval; or when the first time absorbance value of the to-be-measured reaction curve is greater than the first time absorbance value of the target reaction curve and the characteristic parameter of the to-be-measured reaction curve is greater than the characteristic parameter of the target reaction curve, the reaction curve corresponds to the third concentration interval. wherein, is the first time absorbance value of the to-be-measured reaction curve, is the first time absorbance value of the target reaction curve, is the characteristic parameter of the to-be-measured reaction curve, is the characteristic parameter of the target reaction curve, is the time corresponding to the to-be-measured reaction curve when the absorbance value of the to-be-measured reaction curve reaches the absorbance value corresponding to the second time in the target reaction curve, is the time corresponding to the reaction curve with the peak value in the hook effect curve when the absorbance value of the reaction curve with the peak value reaches the absorbance value corresponding to the second time in the target reaction curve, ​The light absorbance value corresponding to the first time of the reaction curve of the peak value in the hook effect curve.

[0012] The serum concentration of the sample solution to be detected is determined according to the true concentration value, including: calculating the serum concentration according to the following formula: ; wherein, The true concentration value is The hematocrit is

[0013] Another technical solution adopted by the present application is to provide a sample analysis device, which comprises a processor and a memory, the memory is used to store program data, and the processor is used to execute the program data to realize the method as described above.

[0014] Another technical solution adopted by the present application is to provide a computer readable storage medium, which stores program data, and the program data is used to realize the method as described above when executed by a processor.

[0015] The sample analysis method provided by the present application comprises: obtaining a to-be-tested reaction curve of a mixed solution; wherein the mixed solution is formed by mixing a sample solution to be detected and a reagent solution, the sample solution to be detected and the reagent solution react to form a mixture, and the reaction curve represents the correspondence between the light absorbance value of the mixture and the time; determining an estimated concentration value of the sample solution to be detected in a pre-established calibration curve based on a characteristic parameter of the to-be-tested reaction curve; wherein the calibration curve represents the correspondence between the characteristic parameter and the estimated concentration value; determining a corresponding true concentration value in a pre-established hook effect curve based on the estimated concentration value; wherein the hook effect curve represents the correspondence between the estimated concentration value and the true concentration value; and determining the serum concentration of the sample solution to be detected according to the true concentration value. In the above manner, the pre-established calibration curve and hook effect curve are used to detect the concentration value, thereby avoiding the problems of wasting reagents and reducing detection efficiency in the prior art when the dilution method is used for detecting the hook effect of a high-concentration sample solution, thereby reducing the detection cost and improving the detection efficiency. BRIEF DESCRIPTION OF DRAWINGS

[0016] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings needed in the embodiment description will be briefly introduced. Obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative labor. Among them:

[0017] Figure 1 is a curve diagram of the light absorbance and the increase of the antigen in the existing sample detection;

[0018] Figure 2is a corresponding diagram of the relationship between the existing measured concentration and the actual concentration;

[0019] Figure 3 is a flowchart of a first embodiment of the sample analysis method provided by the present application;

[0020] Figure 4 is a diagram of the calibration curve provided by the present application;

[0021] Figure 5 is a diagram of the target reaction curve of the target sample solution provided by the present application;

[0022] Figure 6 is a diagram of the hook effect curve provided by the present application;

[0023] Figure 7 is a flowchart of a second embodiment of the sample analysis method provided by the present application;

[0024] Figure 8 is a structural diagram of an embodiment of the sample analysis device provided by the present application;

[0025] Figure 9 is a structural diagram of an embodiment of the computer readable storage medium provided by the present application. DETAILED DESCRIPTION

[0026] The technical solutions in the embodiments of the present application will be described clearly and completely below with reference to the drawings in the embodiments of the present application. It can be understood that the specific embodiments described herein are only used to explain the present application, but not to limit the present application. In addition, it should be noted that, for the convenience of description, only the parts related to the present application are shown in the drawings, but not all the structures. Based on the embodiments in the present application, all the other embodiments obtained by those skilled in the art without creative labor fall within the scope of the present application.

[0027] The terms "first", "second", and the like in the present application are used to distinguish different objects, but not to describe a specific order. In addition, the terms "include" and "have" and any variations thereof are intended to cover non-exclusive inclusion. For example, a process, method, system, product or device including a series of steps or units is not limited to the listed steps or units, but optionally includes other steps or units not listed or optionally includes other steps or units inherent to the process, method, product or device.

[0028] Reference to "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment can be included in at least one embodiment. The appearances of the phrase "in one embodiment" in various places in the specification are not necessarily all referring to the same embodiment, nor are they necessarily all referring to a common set of embodiments. One of ordinary skill in the art will readily recognize from the disclosure herein that embodiments of the present application can be combined with embodiments of the other applications.

[0029] In the field of sample detection, the detection of immune turbidity, the conventional method includes scattering immune turbidimetry and transmission immune turbidimetry, the main principle of which is to use a light source to irradiate the reaction solution of antigen and antibody (such as a mixed solution formed by adding reagents to the sample to be detected), and to use a receiver at the other end of the reaction cup to collect the transmitted light or scattered light and convert it into a signal value, and then according to the curve obtained with the signal value over time, the concentration of the antigen solution can be calculated.

[0030] However, in the actual detection process, it is limited by the concentration ratio of antigen and antibody. When different concentrations of antigens are added to a constant dose of antibody solution, the amount of immune complex formed will increase with the increase of antigen concentration, and when it reaches the peak, the amount of immune complex will decrease with the increase of antigen concentration, resulting in a bell-shaped curve as shown in Figure 1 , which is the content expressed by the famous Heidelberg curve.

[0031] Referring to Figure 1 , it can be seen from the figure that the peak part of the curve is the appropriate range of antigen and antibody ratio, which is called the zone of equivalence of antigen and antibody reaction, also known as the equilibrium zone. In this range, the antigen and antibody are fully combined to form the most immune complex, that is, the most precipitate, and the ratio of antigen and antibody is the most appropriate, which is called the optimal ratio. Before and after the zone of equivalence, there are antibody excess zone and antigen excess zone, respectively, and in these two ranges, the formation of precipitate will be affected, which is called zone phenomenon. When the antibody is excessive, it is called prezone, and when the antigen is excessive, it is called postzone. Whether in the prezone or the postzone, false negative results will occur, resulting in the actual antigen concentration measured as shown by the dotted curve in Figure 2 , resulting in an incorrect result. Therefore, in order to minimize false negative results and measure the ideal concentration of the solid curve in Figure 2 , it is necessary to incorporate the detection of antigen excess into the immune turbidity detection method. The conventional methods include pre-reaction before detection, addition of antigens consistent with the substance to be detected at the end of the reaction, or use of different recognition methods for different items, etc. However, these methods have the disadvantages of long reaction time and large amount of reagent consumption.

[0032] See Figure 3 , Figure 3 This is a flowchart illustrating the first embodiment of the sample analysis method provided in this application. The method includes:

[0033] Step 31: Obtain the actual concentration values ​​of multiple high-concentration sample solutions; wherein the concentration value of the high-concentration sample solution is greater than the preset concentration value.

[0034] Understandably, experiments have shown that high-concentration samples are more prone to the hook effect during the reaction. Therefore, in this embodiment, by modeling the actual concentration value of high-concentration samples to form a hook effect curve, the high-concentration samples can be more accurately detected.

[0035] Specifically, several high-concentration sample points b1, b2…bm, S, c1, c2…cn are selected, where S is a boundary point. In one embodiment, this boundary point is the point with the highest reactivity. Reactivity is a characteristic parameter used to measure reaction efficiency and can be determined from the reaction curve. In some embodiments, reactivity can be the reaction rate.

[0036] Step 32: Determine the estimated concentration values ​​of multiple high-concentration sample solutions based on the characteristic parameters of the reaction curves and the calibration curves; wherein, the calibration curves represent the correspondence between the characteristic parameters and the estimated concentration values.

[0037] The reaction curve refers to the curve showing the relationship between absorbance value and time. The absorbance value is obtained by mixing the sample solution (antibody solution) and the reagent solution (antigen solution), reacting them, irradiating the reaction cell with a light source, and receiving the scattered / transmitted signals using a receiver for further processing. The absorbance value can reflect the amount of conjugate in the mixed solution, and can further be used to calculate the concentration of the original sample solution.

[0038] Among them, the characteristic parameter is a parameter used to represent the characteristics of the above reaction curve. Specifically, the characteristic parameter mainly represents the reaction efficiency corresponding to the reaction curve, such as the reaction rate.

[0039] The calibration curve is used to represent the correspondence between characteristic parameters and estimated concentration values. Optionally, in one embodiment, the calibration curve can be obtained in the following way:

[0040] Obtain the concentration values ​​of multiple calibration sample solutions; and obtain the characteristic parameters of the reaction curves corresponding to the mixing of multiple calibration sample solutions with reagent solutions; establish calibration curves based on multiple calibration concentration values ​​and corresponding characteristic parameters.

[0041] like Figure 4 As shown, Figure 4This is a schematic diagram of the calibration curve of this application. The horizontal axis of the calibration curve represents the estimated concentration value, and the vertical axis represents the characteristic parameters.

[0042] Specifically, k calibration sample solutions with concentration points (a1, a2...ak) are set, and the k calibration sample solutions are mixed with reagent solutions to obtain k reaction curves. Then, k reactivity degrees are determined from the k reaction curves, and the mapping relationship between reactivity degree and estimated concentration is constructed, i.e., the calibration curve.

[0043] Understandably, in one embodiment, the selection of the above k concentration points can be less than the preset concentration value in step 31 above. That is, a1, a2...ak and b1, b2...bm, S, c1, c2...cn constitute three intervals.

[0044] Optionally, in this step, some important parameters can be obtained further based on the reaction curve of the calibration sample solution with the highest concentration value, for use in subsequent sample testing. Specifically:

[0045] Among multiple calibration sample solutions, obtain the target reaction curve corresponding to the target sample solution with the highest concentration value; determine the absorbance value at the first time point and the absorbance value at the second time point in the target reaction curve; wherein, the absorbance value at the second time point is greater than that at the first time point.

[0046] Specifically, such as Figure 5 As shown, Figure 5 This is a schematic diagram of the target reaction curve of the target sample solution in this application, where the horizontal axis represents time and the vertical axis represents absorbance value. Specifically, the absorbance value corresponding to time t is determined. and the absorbance value at time T (T>t) .

[0047] Furthermore, the above-mentioned high-concentration sample solutions were mixed and reacted with the reagent solution to obtain the corresponding reaction curves, and the characteristic parameters (reactivity) corresponding to each high-concentration sample solution were further obtained.

[0048] Since the calibration curves described above represent the mapping relationship between characteristic parameters and estimated concentrations, the estimated concentration value of each high-concentration sample solution can be obtained by further analyzing the characteristic parameters of each high-concentration sample solution.

[0049] Step 33: Establish a hook effect curve based on the actual and estimated concentration values ​​of multiple high-concentration sample solutions.

[0050] Understandably, by combining steps 31 and 32 above, we obtain the actual concentration values ​​and estimated concentration values ​​corresponding to multiple high-concentration sample solutions, and thus establish the correspondence between the actual concentration values ​​and the estimated concentration values.

[0051] Optionally, in further embodiments, the hook-shaped effect curve can also be divided into multiple intervals, so that when detecting the concentration of the sample solution to be detected, different intervals are used for detection according to different sample solutions to be detected.

[0052] As shown in Figure 6 , Figure 6 is a schematic diagram of the hook-shaped effect curve provided by the present application. In this embodiment, three intervals are taken as an example:

[0053] The first interval: a1, a2…ak;

[0054] The second interval: b1, b2…bm;

[0055] The third interval: c1, c2…cn;

[0056] Among them, the demarcation point of the second interval and the third interval is S, S is the point corresponding to the sample pair with the largest reaction degree in the multiple high-concentration sample solutions.

[0057] It can be understood that the division of the above three intervals is only an embodiment, and in other embodiments, more intervals can also be divided according to the reaction characteristics, which is not limited here.

[0058] Step 34: detecting the concentration of the sample solution to be detected by using the calibration curve and the hook-shaped effect curve.

[0059] Different from the prior art, the sample analysis method provided by the present application comprises: obtaining the true concentration values of multiple high-concentration sample solutions; wherein the concentration value of the high-concentration sample solution is greater than a preset concentration value; determining the estimated concentration values of the multiple high-concentration sample solutions according to the characteristic parameters of the reaction curves of the multiple high-concentration sample solutions and the calibration curve; wherein the calibration curve represents the corresponding relationship between the characteristic parameters and the estimated concentration values; establishing a hook-shaped effect curve according to the true concentration values and the estimated concentration values of the multiple high-concentration sample solutions; and detecting the concentration of the sample solution to be detected by using the calibration curve and the hook-shaped effect curve. Through the above-mentioned manner, the calibration curve and the hook-shaped effect curve are used to detect the concentration value, which avoids the problem of wasting reagents and reducing the detection efficiency in the prior art when the dilution method is used for detecting the hook-shaped effect of the high-concentration sample solution, thereby reducing the detection cost and improving the detection efficiency.

[0060] The above embodiments mainly introduce the establishment process of the hook-shaped effect curve, and the following will introduce the concentration detection of the sample solution by using the hook-shaped effect interval.

[0061] Referring to Figure 7 , Figure 7 is a flowchart of the second embodiment of the sample analysis method provided by the present application, which comprises:

[0062] Step 71: Obtain a to-be-tested reaction curve of the mixed solution.

[0063] The mixed solution is formed by mixing a to-be-tested sample solution and a reagent solution, the to-be-tested sample solution and the reagent solution react to form a mixture, and the reaction curve represents a corresponding relationship between an absorbance value of the mixture and time.

[0064] Optionally, in an embodiment, the to-be-tested sample solution is an antibody solution, the reagent solution is an antigen solution, the antibody solution and the antigen solution react after being mixed to generate an immune conjugate, the concentration of the antibody solution is further detected by irradiating the mixed solution with a light source and collecting reflected / transmitted light to detect the absorbance.

[0065] Step 72: Determine an estimated concentration value of the to-be-tested sample solution in a pre-established calibration curve based on a characteristic parameter of the to-be-tested reaction curve.

[0066] The calibration curve represents a corresponding relationship between the characteristic parameter and the estimated concentration value, and the calibration curve can be established in the manner of the above embodiments.

[0067] Step 73: Determine a corresponding true concentration value in a pre-established hook effect curve based on the estimated concentration value.

[0068] The hook effect curve represents a corresponding relationship between the estimated concentration value and the true concentration value.

[0069] Optionally, in an embodiment, since the hook effect curve is divided into a plurality of different intervals, a reference interval can be determined according to the characteristics of the reaction curve, specifically as follows:

[0070] When the following conditions are met: and the reaction curve is determined to correspond to a first concentration interval; or

[0071] When the following conditions are met: and the reaction curve is determined to correspond to a second concentration interval; or

[0072] When the following conditions are met: and the reaction curve is determined to correspond to a third concentration interval.

[0073] Wherein, is an absorbance value of the to-be-tested reaction curve at a first time, is an absorbance value of the target reaction curve at the first time, is a characteristic parameter corresponding to the to-be-tested reaction curve, is a characteristic parameter corresponding to the target reaction curve, This refers to the time when the absorbance value of the test reaction curve reaches the absorbance value corresponding to the second time point in the target reaction curve. This refers to the time when the absorbance value of the reaction curve corresponding to the peak in the hook effect curve reaches the absorbance value corresponding to the second time step in the target reaction curve. This represents the absorbance value of the reaction curve at the first moment corresponding to the peak value in the hook effect curve.

[0074] Furthermore, when determining the first interval of the sample solution to be tested, its estimated concentration value is directly used as the true concentration value. When determining the second or third interval of the sample solution to be tested, the mapping relationship on the hook effect curve is used to determine the true concentration value corresponding to its estimated concentration value.

[0075] Step 74: Determine the serum concentration of the sample solution to be tested based on the actual concentration value.

[0076] Understandably, the actual concentration values ​​determined in the above embodiments are whole blood concentrations. In practical applications, serum concentration values ​​are often required and can be calculated using the following formula:

[0077] ;

[0078] in, This represents the true concentration value (i.e., the whole blood concentration value). This is hematocrit.

[0079] Understandably, in the above embodiments, for the same type of instruments, samples, and reagents, the establishment of calibration curves and hook effect curves can be done once. If the sample or reagent is changed, the process of the first embodiment above needs to be repeated to re-establish the calibration curves and hook effect curves.

[0080] Unlike existing technologies, the sample analysis method provided in this application includes: obtaining the reaction curve of the mixed solution; determining the estimated concentration value of the sample solution to be tested in a pre-established calibration curve based on the characteristic parameters of the reaction curve; determining the corresponding true concentration value in a pre-established hook effect curve based on the estimated concentration value; and determining the serum concentration of the sample solution to be tested based on the true concentration value. By utilizing the pre-established calibration curve and hook effect curve to detect the concentration value, this method avoids the problems of wasted reagents and reduced detection efficiency associated with dilution methods used in existing technologies for detecting hook effects in high-concentration sample solutions, thereby reducing detection costs and improving detection efficiency.

[0081] See Figure 8 , Figure 8FIG. 1 is a structural schematic diagram of an embodiment of a sample analysis device provided by the present application, the sample analysis device comprising a processor 81 and a memory 82, the memory 82 being configured to store program data, and the processor 81 being configured to execute the program data to implement a method as follows:

[0082] obtaining true concentration values of a plurality of high-concentration sample solutions, wherein the concentration values of the high-concentration sample solutions are greater than a preset concentration value; determining estimated concentration values of the plurality of high-concentration sample solutions according to characteristic parameters of reaction curves of the plurality of high-concentration sample solutions and a calibration curve, wherein the calibration curve represents a corresponding relationship between the characteristic parameters and the estimated concentration values; establishing a hook effect curve according to the true concentration values and the estimated concentration values of the plurality of high-concentration sample solutions; and detecting the concentration of a to-be-detected sample solution by using the calibration curve and the hook effect curve. Or

[0083] obtaining a to-be-detected reaction curve of a mixed solution, wherein the mixed solution is formed by mixing a to-be-detected sample solution and a reagent solution, the to-be-detected sample solution and the reagent solution react to form a mixture, and the reaction curve represents a corresponding relationship between absorbance values of the mixture and time; determining an estimated concentration value of the to-be-detected sample solution in a pre-established calibration curve based on a characteristic parameter of the to-be-detected reaction curve, wherein the calibration curve represents a corresponding relationship between the characteristic parameters and the estimated concentration values; determining a corresponding true concentration value in a pre-established hook effect curve based on the estimated concentration value, wherein the hook effect curve represents a corresponding relationship between the estimated concentration values and the true concentration values; and determining a serum concentration of the to-be-detected sample solution according to the true concentration value.

[0084] Referring to Figure 9 , Figure 9 FIG. 2 is a structural schematic diagram of an embodiment of a computer-readable storage medium provided by the present application, the computer-readable storage medium 90 storing program data 91, the program data 91 being configured to implement a method as follows when executed by a processor:

[0085] obtaining true concentration values of a plurality of high-concentration sample solutions, wherein the concentration values of the high-concentration sample solutions are greater than a preset concentration value; determining estimated concentration values of the plurality of high-concentration sample solutions according to characteristic parameters of reaction curves of the plurality of high-concentration sample solutions and a calibration curve, wherein the calibration curve represents a corresponding relationship between the characteristic parameters and the estimated concentration values; establishing a hook effect curve according to the true concentration values and the estimated concentration values of the plurality of high-concentration sample solutions; and detecting the concentration of a to-be-detected sample solution by using the calibration curve and the hook effect curve. Or

[0086] An unknown reaction curve of the mixed solution is obtained; the mixed solution is formed by mixing the sample solution to be detected and the reagent solution, the sample solution to be detected and the reagent solution react to form a mixture, and the reaction curve represents the corresponding relationship between the absorbance value of the mixture and time; based on the characteristic parameter of the unknown reaction curve, an estimated concentration value of the sample solution to be detected is determined in a pre-established calibration curve; the calibration curve represents the corresponding relationship between the characteristic parameter and the estimated concentration value; based on the estimated concentration value, a corresponding true concentration value is determined in a pre-established hook effect curve; the hook effect curve represents the corresponding relationship between the estimated concentration value and the true concentration value; and the serum concentration of the sample solution to be detected is determined according to the true concentration value.

[0087] In several embodiments provided in the present application, it should be understood that the disclosed methods and devices can be implemented in other manners. For example, the above-described device embodiments are merely illustrative, and the division of the modules or units can be different, for example, the division of the modules or units can be combined or integrated into another system, or some features can be ignored or not executed.

[0088] The units described as separate components may or may not be physically separate, and the components shown as units may or may not be physical units, i.e., they can be located in one place or distributed on multiple network units. Some or all of the units can be selected according to actual needs to achieve the purpose of the present embodiment.

[0089] In addition, each functional unit in each embodiment of the present application can be integrated into a processing unit, or each unit can exist physically, or two or more units can be integrated into one unit. The integrated unit can be realized in the form of hardware or in the form of a software functional unit.

[0090] The above description is merely an embodiment of the present application, and does not limit the patent scope of the present application. Any equivalent structure or process transformation according to the content of the present application specification and drawings, or direct or indirect application in other related technical fields, is also included in the patent protection scope of the present application.

Claims

1. A method of sample analysis, characterized by, The method comprises: obtaining a to-be-tested reaction curve of a mixed solution; wherein, the to-be-tested sample solution and the reagent solution react to form the mixed solution, the to-be-tested sample solution and the reagent solution react to form a mixture, the to-be-tested reaction curve represents a corresponding relationship between the absorbance value of the mixture and time, and the to-be-tested sample solution is a high-concentration sample solution with a concentration value greater than a preset concentration value; determining an estimated concentration value of the to-be-tested sample solution in a pre-established calibration curve based on a characteristic parameter of the to-be-tested reaction curve; wherein, the characteristic parameter represents the reaction efficiency corresponding to the reaction curve, and the calibration curve represents a corresponding relationship between the characteristic parameter and the estimated concentration value; determining a corresponding true concentration value in a pre-established hook effect curve based on the estimated concentration value; wherein, the hook effect curve represents a corresponding relationship between the estimated concentration value and the true concentration value; determining the serum concentration of the to-be-tested sample solution according to the true concentration value.

2. The method of claim 1, wherein the method further comprises: obtaining concentration values of a plurality of calibration sample solutions; and obtaining characteristic parameters of reaction curves corresponding to the mixing of the plurality of calibration sample solutions and reagent solutions, respectively; establishing the calibration curve according to the plurality of concentration values and the corresponding characteristic parameters.

3. The method of claim 2, wherein the method further comprises: obtaining a target reaction curve corresponding to a target sample solution with the largest concentration value among the plurality of calibration sample solutions; determining an absorbance value corresponding to a first time and an absorbance value corresponding to a second time in the target reaction curve; wherein, the second time is greater than the first time.

4. The method of claim 3, wherein the method further comprises: obtaining true concentration values of a plurality of high-concentration sample solutions; wherein, the concentration values of the high-concentration sample solutions are greater than a preset concentration value; determining estimated concentration values of the plurality of high-concentration sample solutions according to the characteristic parameters of the reaction curves of the plurality of high-concentration sample solutions and the calibration curve; establishing the hook effect curve according to the true concentration values and the estimated concentration values of the plurality of high-concentration sample solutions.

5. The method of claim 4, wherein the hook effect curve is divided into a plurality of concentration intervals based on the true concentration values; and the determining of the corresponding true concentration value in the pre-established hook effect curve based on the estimated concentration value comprises: determining a concentration interval corresponding to the to-be-tested reaction curve; and determining a true concentration value corresponding to the estimated concentration value within the concentration interval corresponding to the to-be-tested reaction curve.

6. The method of claim 5, wherein ​ ​ ​ ​ ​ The hook effect curve is divided into at least a first concentration interval, a second concentration interval and a third concentration interval based on the true concentration values, a boundary point of the first concentration interval and the second concentration interval is a true concentration value corresponding to a sample solution corresponding to the preset concentration value, and the second concentration interval and the third concentration interval are true concentration values corresponding to maximum characteristic parameters in reaction curves of the plurality of high-concentration sample solutions. In the first concentration interval, the corresponding estimated concentration value is equal to the true concentration value.

7. The method of claim 6, wherein the concentration interval corresponding to the to-be-detected reaction curve is determined by:

8. The method of claim 1, wherein the serum concentration of the to-be-detected sample solution is determined according to the true concentration value by: In and when the to-be-tested reaction curve corresponds to the first concentration interval; or In and when the to-be-tested reaction curve corresponds to the second concentration interval; or In and when the to-be-tested reaction curve corresponds to the third concentration interval, determining that the to-be-tested reaction curve corresponds to the third concentration interval; wherein, is the absorbance value of the first moment of the target reaction curve, is the absorbance value of the first moment of the target reaction curve, is the characteristic parameter corresponding to the target reaction curve, is the characteristic parameter corresponding to the target reaction curve, is the time corresponding to the absorbance value of the second moment of the target reaction curve when the absorbance value of the measured reaction curve reaches the absorbance value of the second moment of the target reaction curve, is the time corresponding to the absorbance value of the second moment of the target reaction curve when the absorbance value of the first moment of the reaction curve corresponding to the peak value of the hook effect curve reaches the absorbance value of the second moment of the target reaction curve, is the absorbance value of the first moment of the reaction curve corresponding to the peak value of the hook effect curve. The serum concentration is calculated according to the following formula: The sample analysis device comprises a processor and a memory, the memory is used to store program data, and the processor is used to execute the program data to realize the method of any one of claims 1-8. The computer readable storage medium stores program data, and the program data is executed by the processor to realize the method of any one of claims 1-8. ； wherein, is the true concentration value, is the hematocrit.

9. A sample analysis device, characterized by, ​ 10. A computer-readable storage medium, characterized in that, ​

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