Wide field pigeon brain stereotaxic positioning device and operation method thereof

Abstract

The present application relates to the technical field of brain stereotaxy, and discloses a wide-view pigeon brain stereotaxy device and an operating method thereof, which comprises a base plate, the surface of the base plate is fixedly connected with an ear canal positioner, a beak angle positioner and a beak mouth positioner, and the operating method for adjusting the ear canal positioner, installing the beak angle positioner, installing the ear canal positioner on a pigeon, installing a beak rod on a pigeon and adjusting the positions of the beak mouth positioner and a horizontal positioning arm is adopted when in use, the horizontal positioning arm slides on the vertical moving track and the horizontal moving track, the left ear rod and the right ear rod move on the vertical column, and the beak rod slides in the sliding groove, so that the installation precision is improved, the positioning device is prevented from falling off, and the problem that the existing pigeon brain stereotaxy device seriously blocks the field of view of a pigeon is solved.

Description

Technical Field

[0001] This invention relates to the field of brain stereotactic technology, specifically to a wide-field-of-view pigeon brain stereotactic device and its operation method. Background Technology

[0002] Stereotactic brain mapping devices are commonly used research tools for studying neuroanatomy and neurophysiology in various animals. The working principle of a stereotactic brain mapping device is to use specific landmarks on the skull surface as reference points, referencing corresponding brain atlases, and moving in multiple dimensions to reach specific intracranial neural nuclei. This allows for operations such as directional signal detection, electrical stimulation, microinjection, and electrical ablation of the corresponding nuclei. Therefore, the accuracy and repeatability of stereotactic mapping are important indicators of the quality of a stereotactic brain mapping device. Currently, existing stereotactic brain mapping devices, both domestically and internationally, are mainly designed for mammals; there are relatively few devices suitable for birds, especially pigeons. Birds have unique anatomical structures, and their corresponding brain atlases require that, after fixation, the line connecting the pigeon's beak angle and the midline of the ear shaft line forms a 45° angle with the horizontal plane. To date, most domestic pigeon stereotactic brain mapping devices adopt the stereotactic scheme published by Karten in 1967. This scheme uses the pigeon's beak angle as the anterior fixation point and the temporal fossae of the bilateral ear openings as posterior fixation points, ensuring that the line connecting the beak angle and the outer ear forms a 45° angle with the horizontal plane.

[0003] This positioning scheme has a simple structure and can fix the pigeon's head and locate nerve nuclei, but it suffers from incomplete fixation of the pigeon's head, allowing the head to still rotate around the beak rod after fixation. Furthermore, because it's difficult to ensure the same opening angle between the upper and lower beaks in different experiments, its accuracy in repeated positioning is not high. In addition, the early stereotactic positioning devices had an "I"-shaped column for the ear canal locator, and their beak angle positioning mechanism was distributed on both sides, resulting in a large volume and significantly impacting the pigeon's lateral and posterior visual field. Moreover, the base plate of the earlier stereotactic positioning devices had significant redundancy, especially in the part located on the opposite side of the pigeon's frontal visual field that obstructed it. Summary of the Invention

[0004] (a) Technical problems to be solved

[0005] To address the shortcomings of existing technologies, this invention provides a wide-field-of-view pigeon brain stereo positioning device and its operation method, which has advantages such as high positioning accuracy and reduced obstruction to the pigeon's opposite side and front, thus solving the problems mentioned in the background art.

[0006] (II) Technical Solution

[0007] To achieve the above objectives, the present invention provides the following technical solution:

[0008] Another technical problem to be solved by the present invention is to provide a wide-field-of-view pigeon brain stereo positioning device, including a base plate. An ear canal locator, a beak angle locator, and a beak mouth locator are fixedly connected to the surface of the base plate. The base plate is convex in shape. The ear canal locator consists of a column, a left ear rod, a right ear rod, and a first threaded pin. The single-sided beak angle locator consists of a side plate, a beak rod, and a locking threaded pin. The beak mouth locator consists of a horizontal moving track, a vertical moving track, a third threaded pin, and a fourth threaded pin. A horizontal positioning arm is provided at the front end of the beak mouth locator. The horizontal positioning arm consists of a pressure-adjustable extrusion ring, an adjusting threaded pin, and a beak mouth positioning head. A bottom support is fixedly connected to the bottom of the base plate.

[0009] Preferably, the ear canal locator is fixed on the base plate, the upright is in the shape of a "7", there are two uprights, each can slide along the track and are symmetrically distributed about the base plate, the upright has a square through hole at a 45° angle to the horizontal plane, the cross-section of the left ear rod and the right ear rod are both square, the left ear rod and the right ear rod are inserted into the square through hole of the upright, the outer side of the upright is threaded to the first threaded nail, the end of the first threaded nail abuts against the surface of the left ear rod and the right ear rod, the surface of the left ear rod and the right ear rod is provided with a scale.

[0010] Preferably, the side plate has a square through hole on its surface, which is at a 45° angle to the horizontal plane. The square through hole is connected to the left ear rod. The side plate has a sliding groove at a 45° angle to the horizontal plane. The beak rod slides inside the sliding groove. One end of the beak rod is threadedly connected to a locking screw.

[0011] Preferably, the beak positioner is set on the vertical line of the column connection, the horizontal moving track is fixedly connected to the surface of the base plate, the horizontal moving track is provided with a slide rail inside, the surface of the slide rail is fixedly connected to the vertical moving track, the third threaded pin is installed on the slide rail, and the fourth threaded pin is installed on the vertical moving track.

[0012] Preferably, the horizontal positioning arm slides on the vertical moving track, the beak positioning head is fixed to the vertical moving track by a fourth threaded pin, and the beak positioning head is detachably connected to the pressure adjustable extrusion ring by adjusting the threaded pin.

[0013] A method for operating a wide-field-of-view pigeon brain stereotactic device includes the following steps:

[0014] 1) Adjust the position of the posts: Adjust the two posts along the bottom track to the appropriate position according to the size of the pigeon and fix them.

[0015] 2) Adjust the ear canal locator: Loosen the first threaded pin at the top of the ear canal locator post, increase the distance between the left and right ear rods, and first fix the position of the left ear rod by the first threaded pin on the left post.

[0016] 3) Install the beak locator: Install the beak locator on the ear bar opposite the eye of the pigeon being tested, and make it fit tightly against the corresponding post. Then loosen the locking screw and push the beak bar to the side closest to the base plate.

[0017] 4) Install ear canal locators on pigeons: Fix the pigeon's left ear canal to the left ear bar; then adjust the position of the other right ear bar until it lightly touches the right ear canal for fixation; finally, observe the scales on the left and right ear bars, loosen the first threaded pin and finely adjust the positions of the left and right ear bars until they are symmetrical before fixing them.

[0018] 5) Install the beak bar on the pigeon: Separate the upper and lower beaks of the pigeon, insert the beak bar into it and move it along the groove to the beak corner, and fix the position by locking the threaded pin.

[0019] 6) Adjust the position of the beak locator and the horizontal positioning arm: Adjust the position of the pigeon beak locator on the horizontal moving track so that the beak positioning head is located in the middle of the nose and the tip of the upper beak, and fix the current horizontal position with the third threaded pin; adjust the position of the horizontal positioning arm on the vertical moving track so that the pigeon's upper beak is inserted into the beak positioning head, and then fix the position of the horizontal positioning arm with the fourth threaded pin; then rotate the adjusting threaded pin to adjust the pressure of the pressure adjustable compression ring on the pigeon's maxilla, so as to achieve complete fixation of the pigeon's brain.

[0020] Preferably, in step three, when installing the ear canal locator, the pigeon needs to be anesthetized first, and then the periphery of the pigeon's ear canal needs to be disinfected.

[0021] (III) Beneficial Effects

[0022] Compared with the prior art, the present invention provides a wide-field-of-view pigeon brain stereotactic device and its operation method, which has the following beneficial effects:

[0023] 1. The wide-field-of-view pigeon brain stereo positioning device and its operation method, by adopting a beak angle locator, a beak beak locator, a movable column, and a horizontal positioning arm, in use, the horizontal positioning arm slides on the vertical and horizontal moving tracks, the column moves on the base plate, the left and right ear rods move on the column, and the beak rod slides inside the groove. When fixing the pigeon brain, it achieves the purpose of adapting to pigeons of various sizes, improving positioning accuracy, and preventing the positioning device from falling off.

[0024] 2. The wide-field-of-view pigeon brain stereo positioning device and its operation method, by setting a convex base plate and using a single-sided beak angle locator and a "7"-shaped column, solve the problem of severe obstruction of the pigeon's field of vision by existing pigeon brain stereo positioning devices. Attached Figure Description

[0025] Figure 1 This is a three-dimensional structural diagram of the present invention;

[0026] Figure 2 This is a top view of the structure of the present invention;

[0027] In the diagram: 1. Base plate; 2. Column; 31. Left ear rod; 32. Right ear rod; 4. First threaded pin; 51. Side plate; 52. Beak rod; 54. Slide groove; 55. Locking threaded pin; 61. Horizontal moving track; 62. Vertical moving track; 71. Third threaded pin; 72. Fourth threaded pin; 81. Pressure adjustable extrusion ring; 82. Adjusting threaded pin; 83. Beak positioning head; 9. Bottom support. Detailed Implementation

[0028] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0029] Embodiment: A wide - field pigeon brain stereotaxic apparatus, comprising a bottom plate 1. On the surface of the bottom plate 1, an ear canal locator, a rostral angle locator, and a rostral tip locator are fixedly connected. The bottom plate is in a "convex" shape. The ear canal locator consists of a column 2, a left ear rod 31, a right ear rod 32, and a first screw 4. The ear canal locator is fixed on the bottom plate 1. The column 2 is in a "7" shape. There are two columns 2, which can slide along the bottom track and are symmetrically distributed with respect to the bottom plate 1. A square through - hole is provided on the column 2 at an angle of 45° with the horizontal plane. The cross - sections of the left ear rod 31 and the right ear rod 32 are both square. The left ear rod 31 and the right ear rod 32 are inserted into the square through - hole of the column 2. The outer side of the column 2 is threadedly connected with the first screw 4, and the end of the first screw 4 abuts against the surfaces of the left ear rod 31 and the right ear rod 32. Scales are provided on the surfaces of the left ear rod 31 and the right ear rod 32. The rostral angle locator consists of a side plate 51, a rostral rod 52, and a locking screw 55. A square through - hole is provided on the surface of the side plate 51 at an angle of 45° with the horizontal plane, and the square through - hole is connected to the left ear rod 31. A chute 54 is provided on the side plate 51 at an angle of 45° with the horizontal plane. The rostral rod 52 slides inside the chute 54. One end of the rostral rod 52 is threadedly connected with the locking screw 55. The rostral tip locator consists of a horizontal moving track 61, a vertical moving track 62, a third screw 71, and a fourth screw 72. The rostral tip locator is arranged on the perpendicular bisector of the connection line of the columns 2. The horizontal moving track 61 is fixedly connected to the surface of the bottom plate 1. A slide rail is provided inside the horizontal moving track 61, and the surface of the slide rail is fixedly connected to the vertical moving track 62. The third screw 71 is installed on the slide rail, and the fourth screw 72 is installed on the vertical moving track 62. A horizontal positioning arm is provided at the front end of the rostral tip locator. The horizontal positioning arm consists of a pressure - adjustable pressing ring 81, an adjusting screw 82, and a rostral tip positioning head 83. The horizontal positioning arm slides on the vertical moving track 62. The rostral tip positioning head 83 is fixed to the vertical moving track 62 through the fourth screw 72. The rostral tip positioning head 83 is detachably connected to the pressure - adjustable pressing ring 81 through the adjusting screw 82. The bottom of the bottom plate 1 is fixedly connected with a bottom support 9.

[0030] An operation method of a wide - field pigeon brain stereotaxic apparatus includes the following steps:

[0031] Step 1: Adjust the position of the columns: According to the size of the pigeon, adjust the two - side columns (2) to a suitable position along the bottom track and fix them.

[0032] Step 2: Adjust the ear canal locator: Loosen the first screw 4 at the top of the column of the ear canal locator, pull apart the distance between the left ear rod 31 and the right ear rod 32, and first fix the position of the left ear rod 31 through the first screw 4 on the left - hand column 2.

[0033] Step 3: Install the beak locator: Install the beak locator on the ear bar opposite the eye of the pigeon being tested, and make it fit snugly against the corresponding post 2. Then loosen the locking screw 55 and push the beak bar 52 to the side closest to the base plate 1.

[0034] Step 4: Install ear canal locators on pigeons: First, anesthetize the pigeons, then disinfect the outer periphery of the pigeons' ear canals, and fix the pigeons' left ear canal to the left ear rod 31; then adjust the position of the other right ear rod 32 until it lightly touches the right ear canal for fixation; finally, observe the scales on the left ear rod 31 and the right ear rod 32, loosen the first threaded pin 4 and finely adjust the positions of the left ear rod 31 and the right ear rod 32 until the left and right positions are symmetrical, and then fix them.

[0035] Step 5: Install the beak rod 52 on the pigeon: Separate the upper and lower beaks of the pigeon, insert the beak rod 52 into it and move it along the slide 54 to the beak corner, and fix the position by locking the threaded pin 55.

[0036] Step Six: Adjust the position of the beak locator and the horizontal positioning arm: Adjust the position of the pigeon beak locator on the horizontal moving track 61 so that the beak positioning head 83 is located in the middle of the nose and the tip of the upper beak, and fix the current horizontal position with the third threaded pin 71; adjust the position of the horizontal positioning arm on the vertical moving track 62 so that the pigeon's upper beak is inserted into the beak positioning head 83, and then fix the position of the horizontal positioning arm with the fourth threaded pin 72; then rotate the adjusting threaded pin 82 to adjust the pressure of the pressure adjustable compression ring 81 on the pigeon's maxilla, so as to achieve complete fixation of the pigeon's brain.

[0037] The beneficial effects of this invention are as follows: This wide-field-of-view pigeon brain stereo positioning device and its operating method, by employing a beak angle locator, a beak beak locator, a movable column, and a horizontal positioning arm, achieves improved installation accuracy and prevents the positioning device from falling off when installing the pigeon by using the horizontal positioning arm to slide on the vertical moving track 62 and the horizontal moving track 61 during use, the column moves on the base plate, the left ear rod 31 and the right ear rod 32 move on the column 2, and the beak rod 52 slides inside the sliding groove 54. By setting a convex base plate 1, using a single-sided beak angle locator and a "7"-shaped column, the problem of severe obstruction of the pigeon's field of vision by existing pigeon brain stereo positioning devices is solved.

[0038] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A wide-field-of-view pigeon brain stereoscopic positioning device, comprising a base plate (1), characterized in that: The base plate (1) is fixedly connected to an ear canal locator, a beak angle locator, and a beak mouth locator. The base plate is convex in shape. The ear canal locator consists of a column (2), a left ear rod (31), a right ear rod (32), and a first threaded pin (4). The column (2) is 7-shaped. There are two columns (2), which can slide along the track and are symmetrically distributed about the base plate (1). The beak angle locator is installed on the ear rod of one side of the column. The beak angle locator is composed of... The beak positioner consists of a side plate (51), a beak rod (52), and a locking screw (55). The beak positioner is composed of a horizontal moving track (61), a vertical moving track (62), a third screw (71), and a fourth screw (72). The front end of the beak positioner is provided with a horizontal positioning arm, which is composed of a pressure adjustable extrusion ring (81), an adjusting screw (82), and a beak positioning head (83). The bottom of the base plate (1) is fixedly connected to a bottom support (9).

2. The wide-field-of-view pigeon brain stereoscopic positioning device according to claim 1, characterized in that: The ear canal locator is fixed on the base plate (1). The upper end of the column (2) is provided with a square through hole at a 45° angle to the horizontal plane. The cross-section of the left ear rod (31) and the right ear rod (32) are both square. The left ear rod (31) and the right ear rod (32) are inserted into the square through hole of the column (2). The outer side of the column (2) is threadedly connected to the first threaded nail (4). The end of the first threaded nail (4) abuts against the surface of the left ear rod (31) and the right ear rod (32). The surface of the left ear rod (31) and the right ear rod (32) is provided with a scale.

3. The wide-field-of-view pigeon brain stereoscopic positioning device according to claim 1, characterized in that: The side plate (51) has a square through hole on its surface, which is at a 45° angle to the horizontal plane. The square through hole is connected to the left ear rod (31). The side plate (51) has a sliding groove (54) at a 45° angle to the horizontal plane. The beak rod (52) slides inside the sliding groove (54). One end of the beak rod (52) is threadedly connected to the locking screw (55).

4. The wide-field-of-view pigeon brain stereoscopic positioning device according to claim 1, characterized in that: The beak positioner is set on the vertical line of the line connecting the columns (2). The horizontal moving track (61) is fixedly connected to the surface of the base plate (1). The horizontal moving track (61) is provided with a slide rail inside. The surface of the slide rail is fixedly connected to the vertical moving track (62). The third threaded nail (71) is installed on the slide rail. The fourth threaded nail (72) is installed on the vertical moving track (62).

5. A wide-field-of-view pigeon brain stereoscopic positioning device according to claim 1, characterized in that: The horizontal positioning arm slides on the vertical moving track (62), the beak positioning head (83) is fixed to the vertical moving track (62) by the fourth threaded pin (72), and the beak positioning head (83) is detachably connected to the pressure adjustable extrusion ring (81) by adjusting the threaded pin (82).

6. The method of operating a wide-field-of-view pigeon brain stereotactic device according to any one of claims 1-5, characterized in that: Includes the following steps: 1) Adjust the position of the uprights: According to the size of the pigeon, adjust the two uprights (2) along the bottom track to a suitable position and fix them; 2) Adjust the ear canal locator: Loosen the first threaded pin (4) at the top of the ear canal locator post, pull apart the distance between the left ear rod (31) and the right ear rod (32), and first fix the position of the left ear rod (31) by the first threaded pin (4) on the left post (2); 3) Install the beak locator: Install the beak locator on the ear bar opposite the eye of the pigeon being tested, and make it close to the corresponding post (2). Then loosen the locking screw (55) and push the beak bar (52) to the side closest to the base plate (1). 4) Install ear canal locators on pigeons: Fix the pigeon's left ear canal to the left ear bar (31); then adjust the position of the other right ear bar (32) until it lightly touches the right ear canal for fixation; finally observe the scales of the left ear bar (31) and the right ear bar (32), loosen the first threaded pin (4) and finely adjust the positions of the left ear bar (31) and the right ear bar (32) until the left and right positions are symmetrical before fixing them; 5) Install the beak rod (52) on the pigeon: Separate the upper and lower beaks of the pigeon, put the beak rod (52) into it and move it along the slide (54) to the beak corner, and fix the position by locking the threaded nail (55); 6) Adjust the position of the beak locator and the horizontal positioning arm: Adjust the position of the pigeon beak locator on the horizontal moving track (61) so that the beak positioning head (83) is located in the middle of the nose and the tip of the upper beak. Fix the current horizontal position by the third threaded nail (71); adjust the position of the horizontal positioning arm on the vertical moving track (62) so that the upper beak of the pigeon is inserted into the beak positioning head (83). Then fix the position of the horizontal positioning arm by the fourth threaded nail (72); then rotate the adjusting threaded nail (82) to adjust the pressure of the pressure adjustable compression ring (81) on the pigeon's maxilla to achieve complete fixation of the pigeon's brain.

7. The operation method of the wide-field-of-view pigeon brain stereoscopic positioning device according to claim 6, characterized in that: In step 3, when installing the ear canal locator, the pigeon needs to be anesthetized first, and then the outer periphery of the pigeon's ear canal needs to be disinfected.

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