A sampling device and method for detecting flavonoid substances in wine

Abstract

This invention discloses a sampling device and method for detecting flavonoids in wine, including a sampling box, a sealing cap, and a PLC controller. A sampling suction tube and a venting tube are slidably connected to the lower side of the sampling box along the vertical direction. The sampling box contains a telescopic drive adjustment component for extending or retracting the sampling suction tube and the venting tube. The left and right sides of the sampling box are respectively equipped with dextran gel columns for adsorbing flavonoids into the sampled liquid and an air intake / exhaust component for absorbing and expelling inert gas from the venting tube. During wine sampling, the venting tube and the air intake / exhaust component work together to expel air from the connection between the sealing cap and the vacuum cover, creating a vacuum environment. This ensures that the wine in the barrel is isolated from the outside air during sampling, thus protecting the wine in the barrel.

Description

Technical Field

[0001] This invention relates to the field of sampling technology, specifically to a sampling device and method for detecting flavonoids in wine. Background Technology

[0002] Flavonoids are widely distributed in nature, mainly in vegetables, fruits, and medicinal plants. They can be classified into total flavonoids, isoflavones, flavonols (and their glycosides), and anthocyanins. Flavonoids are secondary metabolites commonly found in many plants. They possess numerous pharmacological activities, such as antibacterial, antiviral, antitumor, antioxidant, and anti-cardiovascular disease effects. Studies have shown that wine is rich in flavonoids; however, it is unclear whether the content of these components varies among different varieties and vintages of wine. Therefore, wine sampling and testing are necessary. However, current sampling techniques present the following problems:

[0003] 1. Existing technology for sampling wine usually involves directly removing the stopper of the barrel, then taking a certain amount of wine from the barrel, and then screening and testing the flavonoids in the wine. The entire sampling process is cumbersome, complicated and inefficient.

[0004] 2. When sampling wine, directly removing the barrel cap can easily expose the wine inside to the air, affecting its quality. Summary of the Invention

[0005] (a) Technical problems to be solved

[0006] The purpose of this invention is to provide a sampling device and sampling method for detecting flavonoids in wine in order to solve the above-mentioned problems.

[0007] (II) Technical Solution

[0008] To achieve the above objectives, the present invention provides the following technical solution:

[0009] The present invention provides a sampling device for detecting flavonoids in wine, comprising a sampling box, a sealing cap and a PLC controller;

[0010] The sampling box has a sampling tube and a venting tube slidably connected to its lower side along the vertical direction. The sampling box is equipped with a telescopic drive adjustment component for driving the sampling tube and venting tube to extend or retract from the sampling box. The left and right sides of the sampling box are respectively equipped with a dextran gel column for adsorbing flavonoids in the sampled liquid and an air intake and exhaust component for venting the venting tube to draw in and expel inert gas. A vacuum cover is provided on the side of the sampling box near the sampling tube and the venting tube. The end of the sampling tube extending into the sampling box is detachably connected to the dextran gel column through the sampling tube structure.

[0011] The sealing plug includes a plug body, with a rotating platform rotatably mounted on the upper side of the plug body. Two second through holes are symmetrically distributed along the vertical direction of the rotating platform. Two first through holes are symmetrically distributed along the rotating platform's central axis on the lower side of the plug body. After the rotating platform rotates to a certain angle, the two second through holes and the two first through holes are connected one-to-one. When the vacuum cover and the plug body are connected, the air intake and exhaust assembly can exhaust the gas between the vacuum cover and the plug body through the vent pipe to form a vacuum environment. An angle adjustment assembly for adjusting the rotation angle of the rotating platform is provided on the side of the sampling box near the sampling suction pipe and the vent pipe.

[0012] Furthermore, the telescopic drive adjustment assembly includes a motor fixedly installed on the top side inside the sampling box. The output shaft of the motor faces downward and is fixedly connected to a lead screw along the central axis. Two guide rods are symmetrically distributed on both sides of the lead screw, with the upper and lower ends of the two guide rods fixedly connected to the inner wall of the sampling box. A slider is threaded onto the lead screw. Guide holes are opened on both sides of the slider to cooperate with the two guide rods to slide and connect with each other. One side of the slider is fixedly connected to the sampling aspiration tube through the sampling tube structure, and the other side of the slider is fixedly connected to the vent pipe through the air inlet and outlet assembly. The output end of the PLC controller is electrically connected to the input end of the motor.

[0013] Furthermore, a threaded connector is provided on the lower side of the plug body, and a circular limiting shoulder plate is formed on the outer side of the connection between the plug body and the threaded connector. An outer cover is provided on the upper outer side of the plug body, and the lower side of the outer cover is connected to the limiting shoulder plate by bolts. A second sealing gasket is provided between the lower side of the outer cover and the limiting shoulder plate, and a third sealing gasket is provided on the lower side of the limiting shoulder plate. An angle adjustment hole is provided at the middle position of the upper side of the rotary table, and a first indicator groove is provided on the upper edge of the rotary table. A second indicator groove corresponding to the first indicator groove is provided on the upper side of the plug body near the rotary table. An annular sealing groove is also provided on the upper side of the plug body to seal and fit with the lower edge of the vacuum cover. An inner annular limiting plate is formed on the inner side of the outer cover to abut against the upper edge of the plug body. A first sealing gasket is provided between the inner annular limiting plate and the upper edge of the plug body. The vacuum cover is made of transparent material.

[0014] Furthermore, the upper side of the plug body has a rotating chamber for cooperating with the rotation of the rotating table. The bottom surface of the rotating chamber has a mounting slot. A tension spring is installed in the mounting slot. The upper and lower ends of the tension spring are respectively fixedly connected to a second rotating joint and a first rotating joint. The second rotating joint is rotatably connected to the middle position of the bottom side of the rotating table, and the first rotating joint is rotatably connected to the bottom surface inside the mounting slot.

[0015] Furthermore, the angle adjustment assembly includes a cavity located inside the lower side of the sampling box. A first bevel gear and a second bevel gear are meshed and connected within the cavity. The shaft end of the first bevel gear is vertical and its lower side is fixedly connected to a first adjusting shaft. The lower end of the first adjusting shaft extends out of the sampling box and is fixedly connected to an angle adjusting plug that mates with the angle adjusting socket. The angle adjusting plug is in the shape of a regular hexagonal prism. The shaft end of the second bevel gear is horizontally positioned and fixedly connected to one end of a second adjusting shaft. The other end of the second adjusting shaft extends out of the sampling box and is fixedly connected to an adjusting knob. When the adjusting knob is rotated, the adjusting knob sequentially drives the angle adjusting plug to rotate via the second adjusting shaft, the second bevel gear, the first bevel gear, and the first adjusting shaft.

[0016] Furthermore, the sampling tube structure includes a sampling tube, one end of which is connected to a sampling aspiration tube. A liquid pump is installed on the sampling tube, and the other end of the sampling tube is connected to an inlet pipe connector. Connecting pipe connectors are respectively connected to both sides of the dextran gel column. A positioning box structure for positioning the dextran gel column is provided on the outer side of the sampling box. A sliding groove for sliding the sampling tube is opened on the outer wall of the sampling box. Under the drive of the telescopic drive adjustment component, the sampling tube slides in the sliding groove, realizing the connection or separation between the inlet pipe connector and the connecting pipe connector. The output terminal of the PLC controller is electrically connected to the input terminal of the liquid pump.

[0017] Furthermore, the positioning box structure includes a box body fixedly installed on the outside of the sampling box. The upper side of the box body has a placement groove for inserting the bottom of the dextran gel column. The bottom surface of the placement groove is provided with four springs arranged in a rectangular array. The lower ends of the four springs are all fixedly connected to the bottom surface of the placement groove. The upper ends of the four springs are connected to each other by a seat plate. The bottom surface of the placement groove is also provided with an L-shaped drain pipe. One end of the drain pipe passes through the box body and is provided with a first solenoid valve. The other end of the drain pipe is connected to a liquid outlet pipe connector that mates with a connecting pipe connector. The upper outer wall of the box body is threaded with a first set bolt for abutting and tightening the outer wall of the dextran gel column. The output terminal of the PLC controller is electrically connected to the input terminal of the first solenoid valve.

[0018] Furthermore, the intake and exhaust assembly includes an inert gas storage tank. An insertion seat for placing the inert gas storage tank is fixedly installed on the outer wall of the sampling box. A second set bolt for abutting and tightening the inert gas storage tank is threaded onto the upper side wall of the insertion seat. An outlet is provided on the upper side of the inert gas storage tank, and one end of a spring-type conduit is connected to the outlet. The other end of the spring-type conduit is connected to the main air pipe and the vent pipe through the main air pipe. The main air pipe and the vent pipe are integrally formed. An air pump is installed on the main air pipe. A branch air pipe is connected between the main air pipes located on both sides of the air pump inlet and outlet. A first three-way valve and a second three-way valve are respectively installed at the connection between the branch air pipe and the main air pipe. An exhaust pipe is connected to the main air pipe located between the air pump outlet and the second three-way valve. A second solenoid valve is installed on the exhaust pipe. The output terminal of the PLC controller is electrically connected to the input terminals of the air pump, the first three-way valve, the second three-way valve, and the second solenoid valve.

[0019] A sampling method for a sampling device for detecting flavonoids in wine includes the following steps:

[0020] S1: Remove the outer cover of the sealing plug from the plug body, and use the telescopic drive adjustment component to retract the sampling tube and vent tube into the sampling box;

[0021] S2: The vacuum cover on the lower side of the sampling box is fitted with the annular sealing groove on the upper side of the plug body. The air intake and exhaust components controlled by the PLC controller exhaust the air in the sealed chamber between the vacuum cover and the plug body to form a vacuum environment.

[0022] S3: In a vacuum environment, the angle adjustment component adjusts the rotation angle of the rotary table so that the two first perforations and the two second perforations are connected to each other. Then, the telescopic drive adjustment component drives the sampling tube and the vent tube to extend and pass through the second perforation and the first perforation in sequence into the barrel. The sampling tube structure draws the wine liquid in the barrel into the dextran gel column. The dextran gel column realizes the adsorption and separation of flavonoids in the wine liquid. The air intake and exhaust component realizes the protection and decompression of the barrel by filling it with inert gas.

[0023] S4: After sampling is completed, the telescopic drive adjustment component drives the sampling suction tube and the vent tube to retract into the sampling box. Then, the angle adjustment component adjusts the rotation of the rotating table to separate the first perforation and the second perforation. Finally, the sampling box is separated from the sealing plug.

[0024] (III) Beneficial Effects

[0025] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0026] 1. The sampling box, sampling tube, positioning box structure and sampling tube structure work together to facilitate the sampling of wine in the barrel and the adsorption and separation of flavonoids by the dextran gel column. The whole process is simple and efficient.

[0027] 2. When sampling wine, the vent pipe and the air intake and exhaust components work together to expel the air between the sealing cap and the vacuum cover to create a vacuum environment, ensuring that the wine inside the barrel is isolated from the outside air during the sampling process, thus protecting the wine inside the barrel.

[0028] 3. The vent pipe and the air intake and exhaust components can also be used to fill the barrel with inert gas, so as to form an inert gas protective layer inside the barrel and reduce pressure during sampling.

[0029] 4. The telescopic drive adjustment component enables the extension or retraction of the sampling tube and the ventilation tube within the sampling box, while also driving the sampling tube structure to connect or separate the sampling tube structure from the dextran gel column, and to fix the position of the dextran gel column. Attached Figure Description

[0030] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0031] Figure 1This is a schematic diagram of the main structure of the present invention;

[0032] Figure 2 This is the present invention. Figure 1 A schematic diagram of the left-side view structure;

[0033] Figure 3 This is the present invention. Figure 1 A schematic diagram of the AA cross-sectional structure;

[0034] Figure 4 This is the present invention. Figure 2 A schematic diagram of the BB cross-sectional structure;

[0035] Figure 5 This is the present invention. Figure 1 A schematic diagram of the three-dimensional structure;

[0036] Figure 6 This is a three-dimensional structural diagram of the plug body of the present invention;

[0037] Figure 7 This is the present invention. Figure 3 A magnified schematic diagram of the structure at point C;

[0038] Figure 8 This is the present invention. Figure 3 A magnified schematic diagram of the structure at point D;

[0039] Figure 9 This is the present invention. Figure 4 A magnified schematic diagram of the structure at point E;

[0040] Figure 10 This is the present invention. Figure 4 A magnified schematic diagram of the structure at point F.

[0041] The reference numerals in the attached drawings are explained as follows: 1. Sampling box; 1a. Box lid; 1b. Handle; 1c. Slide groove; 2. Sampling aspiration tube; 3. Vent tube; 4. Sealing plug; 401. Plug body; 402. Rotating platform; 403. First perforation; 404. Second perforation; 405. Outer cover; 406. Inner annular limiting plate; 407. First sealing gasket; 408. Limiting shoulder plate; 409. Threaded connector; 410. Annular 411. Sealing groove; 412. First indicator groove; 413. Second indicator groove; 414. Angle adjustment socket; 415. First rotary joint; 416. Tension spring; 417. Second rotary joint; 418. Third sealing gasket; 5. Positioning box structure; 501. Box body; 502. First set bolt; 503. Seat plate; 504. Spring; 505. Discharge pipe connector; 506. First solenoid valve; 507. Drain pipe 6. Sampling tube structure; 6a. Sampling tube; 6b. Liquid inlet pipe connector; 6c. Liquid pump; 7. Inlet and outlet assembly; 701. Inert gas storage tank; 702. Main gas pipe; 703. Spring-type conduit; 704. Air pump; 705. First three-way valve; 706. Branch gas pipe; 707. Second three-way valve; 708. Second solenoid valve; 709. Exhaust pipe; 710. Insertion seat; 711. Second set bolt; 8. Angle adjustment assembly; 801. Adjustment knob; 802. First adjustment shaft; 803. Angle adjustment plug; 804. First bevel gear; 805. Second bevel gear; 806. Second adjustment shaft; 9. Telescopic drive adjustment assembly; 9a. Motor; 9b. Lead screw; 9c. Guide rod; 9d. Slider; 10. Dextran gel column; 10a. Connecting pipe connector; 11. Vacuum cover; 12. PLC controller. Detailed Implementation

[0042] To make the objectives, technical solutions, and advantages of this invention clearer, the technical solutions of this invention will be described in detail below. Obviously, the described embodiments are merely some embodiments of this invention, and not all embodiments. Based on the embodiments of this invention, all other implementation methods obtained by those skilled in the art without creative effort are within the scope of protection of this invention.

[0043] See Figure 1-10As shown, this invention provides a sampling device and method for detecting flavonoids in wine, including a sampling box 1, a sealing cap 4, and a PLC controller 12; a sampling suction tube 2 and a venting tube 3 are slidably connected to the lower side of the sampling box 1 along the vertical direction, respectively; a telescopic drive adjustment assembly 9 is provided inside the sampling box 1 for driving the sampling suction tube 2 and the venting tube 3 to extend or retract from the sampling box 1; the telescopic drive adjustment assembly 9 includes a motor 9a fixedly installed on the top side inside the sampling box 1, with the output shaft end of the motor 9a facing downward and a lead screw fixedly connected to the central axis. 9b, two guide rods 9c are symmetrically distributed on both sides of the lead screw 9b with the lead screw 9b as the center. The upper and lower ends of the two guide rods 9c are fixedly connected to the inner wall of the sampling box 1. A slider 9d is threaded on the lead screw 9b. Guide sliding holes are opened on both sides of the slider 9d to cooperate with the two guide rods 9c to slide and connect with each other. One side of the slider 9d is fixedly connected to the sampling suction tube 2 through the sampling tube structure 6. The other side of the slider 9d is fixedly connected to the ventilation tube 3 through the air inlet and outlet assembly 7. The output terminal of the PLC controller 12 is electrically connected to the input terminal of the motor 9a. Through the above-mentioned specific structural design, the output shaft of motor 9a rotates, driving the connected lead screw 9b to rotate. The rotation of lead screw 9b drives the threaded slider 9d to move axially along guide rod 9c, thereby realizing the driving movement of sampling tube 2 and venting tube 3, realizing the extension or retraction of sampling tube 2 and venting tube 3 in sampling box 1, and also realizing the driving of sampling tube structure 6. When sampling tube 2 is extended, sampling tube structure 6 can be connected to dextran gel column 10 and realize the position of dextran gel column 10.

[0044] See instruction manual attached Figure 1 , 4As shown in Figure 10, the sampling box 1 is provided with dextran gel columns 10 for adsorbing flavonoids in the sampled liquid and an inlet / outlet assembly 7 for inhaling and exhaling inert gas through the venting tube 3 on the left and right sides respectively. The inlet / outlet assembly 7 includes an inert gas storage tank 701. An insertion seat 710 for placing the inert gas storage tank 701 is fixedly provided on the outer wall of the sampling box 1. A second setter bolt 711 for abutting and tightening the inert gas storage tank 701 is threaded onto the upper side wall of the insertion seat 710. An outlet is provided on the upper side of the inert gas storage tank 701, and one end of a spring-type conduit 703 is connected to the outlet. The other end of the spring-type conduit 703 is connected to the venting tube 702 through the main venting tube 702. The pipes 3 are interconnected. The main air pipe 702 and the ventilation pipe 3 are integrally formed. An air pump 704 is installed on the main air pipe 702. A branch air pipe 706 is connected between the main air pipes 702 on both sides of the air pump 704's inlet and outlet. A first three-way valve 705 and a second three-way valve 707 are respectively installed at the connection between the branch air pipe 706 and the main air pipe 702. An exhaust pipe 709 is connected to the main air pipe 702 between the air pump 704's outlet and the second three-way valve 707. A second solenoid valve 708 is installed on the exhaust pipe 709. The output terminal of the PLC controller 12 is electrically connected to the input terminals of the air pump 704, the first three-way valve 705, the second three-way valve 707, and the second solenoid valve 708. In practical applications, the intake and exhaust assembly 7 has two operating states:

[0045] First operating state: PLC controller 12 controls the first three-way valve 705 and the second three-way valve 707. At this time, the second solenoid valve 708 is in the closed state, realizing the connection between the main air pipe 702 and the air pump 704 inlet and outlet. The air pump 704 realizes the discharge of inert gas in the inert gas storage tank 701 from the spring-type conduit 703, the main air pipe 702 and the vent pipe 3 in sequence.

[0046] Second operating state: PLC controller 12 controls the first three-way valve 705 and the second three-way valve 707. At this time, the second solenoid valve 708 is in the connected state. The air pump 704 draws in gas through the air pipe 3 and the branch air pipe 706 and discharges the gas through the exhaust pipe 709.

[0047] See instruction manual attached Figure 2 and 4As shown, a vacuum cover 11 is provided on the side of the sampling box 1 near the sampling aspiration tube 2 and the vent tube 3. One end of the sampling aspiration tube 2 extending into the sampling box 1 is detachably connected to the dextran gel column 10 via a sampling tube structure 6. The sampling tube structure 6 includes a sampling tube 6a, one end of which is connected to the sampling aspiration tube 2. A liquid pump 6c is provided on the sampling tube 6a, and the other end of the sampling tube 6a is connected to an inlet connector 6b. Connectors are provided on both sides of the dextran gel column 10. The sampling box 1 has a positioning box structure 5 on its outer side for positioning the dextran gel column 10. A groove 1c is provided on the outer wall of the sampling box 1 to facilitate the sliding of the sampling tube 6a. Driven by the telescopic drive adjustment component 9, the sampling tube 6a slides within the groove 1c, allowing the inlet pipe connector 6b and the connecting pipe connector 10a to connect or disconnect. The output of the PLC controller 12 is electrically connected to the input of the liquid pump 6c. The liquid pump 6c sequentially feeds wine into the dextran gel column 10 through the sampling tube 2, the sampling tube 6a, and the inlet pipe connector 6b. The dextran gel column 10 adsorbs and separates the flavonoids in the wine. After separation, the dextran gel column 10 is removed for testing.

[0048] The positioning box structure 5 includes a box body 501 fixedly installed on the outside of the sampling box 1. The upper side of the box body 501 has a placement groove for inserting the bottom of the dextran gel column 10. The inner bottom surface of the placement groove is provided with four springs 504 arranged in a rectangular array. The lower ends of the four springs 504 are all fixedly connected to the inner bottom surface of the placement groove. The upper ends of the four springs 504 are connected to each other by a seat plate 503. The inner bottom surface of the placement groove is also provided with an L-shaped drain pipe 507. One end of the drain pipe 507 passes through the box body 501 and is provided with a first solenoid valve 506. The other end of the drain pipe 507 is connected to a liquid outlet connector 505 that is connected to the connecting pipe connector 10a. The upper outer wall of the box body 501 is threaded with a first set bolt 502 for abutting and tightening the outer wall of the dextran gel column 10. The output terminal of the PLC controller 12 is electrically connected to the input terminal of the first solenoid valve 506. When the four springs 504 drive the sampling tube structure 6 to move, the inlet pipe connector 6b and the connecting pipe connector 10a of the sampling tube structure 6 are inserted into each other. At this time, the springs 504 are compressed, which can ensure that the inlet pipe connector 6b and the connecting pipe connector 10a are in full contact under the elasticity of the springs 504, and the drain pipe 507 can drain the wine liquid that has passed through the dextran gel column 10.

[0049] See instruction manual attached Figure 3 , 4As shown in Figures 6 and 8, the sealing plug 4 includes a plug body 401. A rotating platform 402 is rotatably mounted inside the upper side of the plug body 401. Two second through holes 404 are symmetrically distributed around the central axis of rotation of the rotating platform 402, extending vertically through the rotating platform 402. Two first through holes 403 are symmetrically distributed around the central axis of rotation of the rotating platform 402, extending vertically through the lower side of the plug body 401. After the rotating platform 402 rotates to a certain angle, the two second through holes 404 and the two first through holes 403 are connected one-to-one. When the vacuum cover 11 and the plug body 401 are connected, the air intake and exhaust assembly 7... A vacuum environment can be formed by venting gas between the vacuum cover 11 and the plug body 401 through the vent pipe 3. A threaded connector 409 is provided on the lower side of the plug body 401. A circular limiting shoulder plate 408 is formed on the outer side of the connection between the plug body 401 and the threaded connector 409. An outer cover 405 is provided on the upper outer side of the plug body 401. The lower side of the outer cover 405 is connected to the limiting shoulder plate 408 by bolts. A second sealing gasket is provided between the lower side of the outer cover 405 and the limiting shoulder plate 408. In practical applications, when no sampling is being performed, the outer cover 405 can improve the sealing of the plug 401. For overall sealing, a third sealing gasket 417 is provided on the lower side of the limiting shoulder plate 408. An angle adjustment hole 413 is provided at the middle of the upper side of the rotating table 402. A first indicator groove 411 is provided on the upper edge of the rotating table 402. A second indicator groove 412 corresponding to and cooperating with the first indicator groove 411 is provided on the upper side of the plug body 401 near the rotating table 402. An annular sealing groove 410 is also provided on the upper side of the plug body 401 to seal and fit with the lower edge of the vacuum cover 11. An inner annular limiting plate 406 is formed on the inner side of the outer cover 405 to abut against the upper edge of the plug body 401. A first sealing gasket 407 is provided between the inner annular limiting plate 406 and the upper edge of the plug body 401. The vacuum cover 11 is made of transparent material. A rotating chamber for cooperating with the rotation of the rotating table 402 is opened inside the upper side of the plug body 401. An installation slot is opened on the bottom surface of the rotating chamber. A tension spring 415 is provided in the installation slot. The upper and lower ends of the tension spring 415 are respectively fixedly connected to a second rotating joint 416 and a first rotating joint 414. The second rotating joint 416 is rotatably connected to the middle position of the bottom side of the rotating table 402, and the first rotating joint 414 is rotatably connected to the bottom surface inside the installation slot.

[0050] An angle adjustment component 8 for adjusting the rotation angle of the rotary table 402 is provided on the side of the sampling box 1 near the sampling aspiration tube 2 and the vent tube 3. The angle adjustment assembly 8 includes a cavity located inside the lower side of the sampling box 1. A first bevel gear 804 and a second bevel gear 805 are meshed and connected to each other inside the cavity. The shaft end of the first bevel gear 804 is vertical and its lower side is fixedly connected to a first adjustment shaft 802. The lower end of the first adjustment shaft 802 extends out of the sampling box 1 and is fixedly connected to an angle adjustment plug 803 that mates with the angle adjustment socket 413. The angle adjustment plug 803 is in the shape of a regular hexagonal prism. The shaft end of the second bevel gear 805 is horizontal and is fixedly connected to one end of a second adjustment shaft 806. The other end of the second adjustment shaft 806 extends out of the sampling box 1 and is fixedly connected to an adjustment knob 801. When the adjustment knob 801 is rotated, the adjustment knob 801 drives the angle adjustment plug 803 to rotate sequentially through the second adjustment shaft 806, the second bevel gear 805, the first bevel gear 804, and the first adjustment shaft 802.

[0051] Working principle:

[0052] When retrieval is required, firstly, remove the outer cover 405 of the sealing plug 4 from the plug body 401. Then, the telescopic drive adjustment assembly 9 retracts the sampling suction tube 2 and the vent tube 3 into the sampling box 1. The vacuum cover 11 on the lower side of the sampling box 1 engages with the annular sealing groove 410 on the upper side of the plug body 401, creating a vacuum environment. The PLC controller 12 controls the air intake and exhaust assembly 7 to expel air from the sealed chamber between the vacuum cover 11 and the plug body 401. In this vacuum environment, the angle adjustment assembly 8 adjusts the rotation angle of the rotary table 402, ensuring that the two first perforations 403 and the two second perforations 404 are connected to each other. Then, the tube extends... The retraction drive adjustment component 9 drives the sampling suction tube 2 and the vent tube 3 to extend and pass through the second perforation 404 and the first perforation 403 into the wine barrel. The sampling tube structure 6 draws the wine liquid from the wine barrel into the dextran gel column 10. The dextran gel column 10 adsorbs and separates the flavonoids in the wine liquid. The air intake and exhaust component 7 fills the wine barrel with inert gas for protection and depressurization. After sampling, the retraction drive adjustment component 9 drives the sampling suction tube 2 and the vent tube 3 to retract into the sampling box 1. Then, the angle adjustment component 8 adjusts the rotation of the rotating table 402 to separate the first perforation 403 and the second perforation 404. Finally, the sampling box 1 is separated from the sealing cap 4.

[0053] The above description is merely a specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed in the present invention should be included within the scope of protection of the present invention. Therefore, the scope of protection of the present invention should be determined by the scope of the claims.

Claims

1. A sampling device for detecting flavonoids in wine, characterized in that: Includes a sampling box (1), a sealing plug (4), and a PLC controller (12); The sampling box (1) is slidably connected to the sampling tube (2) and the venting tube (3) in the vertical direction on the lower side. The sampling box (1) is provided with a telescopic drive adjustment component (9) for driving the sampling tube (2) and the venting tube (3) to extend or retract from the sampling box (1). The left and right sides of the sampling box (1) are respectively provided with a dextran gel column (10) for adsorbing flavonoids in the sampled liquid and an air intake and exhaust component (7) for absorbing and expelling inert gas from the venting tube (3). A vacuum cover (11) is provided on the side of the sampling box (1) near the sampling tube (2) and the venting tube (3). The end of the sampling tube (2) extending into the sampling box (1) is detachably connected to the dextran gel column (10) through the sampling tube structure (6). The sealing plug (4) includes a plug body (401). A rotating platform (402) is rotatably arranged inside the upper side of the plug body (401). Two second perforations (404) are symmetrically distributed along the vertical direction inside the rotating platform (402) with its rotation axis as the center. Two first perforations (403) are symmetrically distributed along the lower side of the plug body (401) with the rotation axis of the rotating platform (402) as the center. After the rotating platform (402) rotates to a certain angle, the two second perforations (404) and the two first perforations (403) are connected one-to-one. When the vacuum cover (11) and the plug body (401) are connected to each other, the air intake and exhaust assembly (7) can exhaust the gas between the vacuum cover (11) and the plug body (401) through the vent pipe (3) to form a vacuum environment. An angle adjustment assembly (8) for adjusting the rotation angle of the rotating platform (402) is provided on the side of the sampling box (1) near the sampling suction pipe (2) and the vent pipe (3).

2. The sampling device for detecting flavonoids in wine according to claim 1, characterized in that: The telescopic drive adjustment assembly (9) includes a motor (9a) fixedly installed on the top side inside the sampling box (1). The output shaft end of the motor (9a) faces downward and is fixedly connected to a lead screw (9b) along the central axis. Two guide rods (9c) are symmetrically distributed on both sides of the lead screw (9b) with the lead screw (9b) as the center. The upper and lower ends of the two guide rods (9c) are fixedly connected to the inner wall of the sampling box (1). A slider (9d) is threaded on the lead screw (9b). Guide sliding holes that are slidably connected to the two guide rods (9c) are opened through both sides of the slider (9d). One side of the slider (9d) is fixedly connected to the sampling suction tube (2) through the sampling tube structure (6). The other side of the slider (9d) is fixedly connected to the ventilation tube (3) through the air intake and exhaust assembly (7). The output end of the PLC controller (12) is electrically connected to the input end of the motor (9a).

3. The sampling device for detecting flavonoids in wine according to claim 1, characterized in that: A threaded connector (409) is provided on the lower side of the plug body (401). A ring-shaped limiting shoulder plate (408) is formed on the outer side of the connection between the plug body (401) and the threaded connector (409). An outer cover (405) is provided on the upper outer side of the plug body (401). The lower side of the outer cover (405) is connected to the limiting shoulder plate (408) by bolts. A second sealing gasket is provided between the lower side of the outer cover (405) and the limiting shoulder plate (408). A third sealing gasket (417) is provided on the lower side of the limiting shoulder plate (408). An angle adjustment hole (413) is opened at the middle position of the upper side of the rotating table (402). The upper edge of the 02) is provided with a first indicator groove (411), and the upper side of the plug body (401) near the rotating table (402) is provided with a second indicator groove (412) that corresponds to and cooperates with the first indicator groove (411). The upper side of the plug body (401) is also provided with an annular sealing groove (410) that cooperates with the lower edge of the vacuum cover (11). The inner side of the outer cover (405) is formed with an inner annular limiting plate (406) that abuts against the upper edge of the plug body (401). A first sealing gasket (407) is provided between the inner annular limiting plate (406) and the upper edge of the plug body (401). The vacuum cover (11) is made of transparent material.

4. The sampling device for detecting flavonoids in wine according to claim 3, characterized in that: The upper side of the plug body (401) is provided with a rotating chamber for cooperating with the rotation of the rotating platform (402). The bottom surface of the rotating chamber is provided with a mounting slot. A tension spring (415) is provided in the mounting slot. The upper and lower ends of the tension spring (415) are respectively fixedly connected to a second rotating joint (416) and a first rotating joint (414). The second rotating joint (416) is rotatably connected to the middle position of the bottom side of the rotating platform (402), and the first rotating joint (414) is rotatably connected to the bottom surface inside the mounting slot.

5. The sampling device for detecting flavonoids in wine according to claim 4, characterized in that: The angle adjustment assembly (8) includes a cavity located inside the lower side of the sampling box (1). A first bevel gear (804) and a second bevel gear (805) are meshed within the cavity. The shaft of the first bevel gear (804) is vertical and its lower side is fixedly connected to a first adjustment shaft (802). The lower end of the first adjustment shaft (802) extends out of the sampling box (1) and is fixedly connected to an angle adjustment plug (803) that mates with the angle adjustment socket (413). The angle adjustment plug (803) has the following shape... It is in the shape of a regular hexagonal prism. The shaft of the second bevel gear (805) is horizontally set and fixedly connected to one end of the second adjusting shaft (806). The other end of the second adjusting shaft (806) passes through the sampling box (1) and is fixedly connected to the adjusting knob (801). When the adjusting knob (801) is rotated, the adjusting knob (801) drives the angle adjusting plug (803) to rotate in sequence through the second adjusting shaft (806), the second bevel gear (805), the first bevel gear (804) and the first adjusting shaft (802).

6. The sampling device for detecting flavonoids in wine according to claim 1, characterized in that: The sampling tube structure (6) includes a sampling tube (6a), one end of which is connected to the sampling aspiration tube (2). A liquid pump (6c) is provided on the sampling tube (6a), and the other end of the sampling tube (6a) is connected to an inlet pipe connector (6b). Connecting pipe connectors (10a) are respectively connected to both sides of the dextran gel column (10). A positioning box structure (5) for positioning the dextran gel column (10) is provided on the outside of the sampling box (1). A sliding groove (1c) for sliding the sampling tube (6a) is provided on the outer wall of the sampling box (1). Under the drive of the telescopic drive adjustment component (9) to the sampling aspiration tube (2), the sampling tube (6a) slides in the sliding groove (1c) to realize the connection or separation between the inlet pipe connector (6b) and the connecting pipe connector (10a). The output end of the PLC controller (12) is electrically connected to the input end of the liquid pump (6c).

7. The sampling device for detecting flavonoids in wine according to claim 6, characterized in that: The positioning box structure (5) includes a box body (501) fixedly installed on the outside of the sampling box (1). The upper side of the box body (501) is provided with a placement groove for inserting the bottom of the dextran gel column (10). The inner bottom surface of the placement groove is provided with four springs (504) arranged in a rectangular array. The lower ends of the four springs (504) are all fixedly connected to the inner bottom surface of the placement groove. The upper ends of the four springs (504) are connected to a seat plate (503). The inner bottom surface of the placement groove is also provided with an L-shaped drainage channel. One end of the drain pipe (507) extends out of the box body (501) and is equipped with a first solenoid valve (506). The other end of the drain pipe (507) is connected to an outlet pipe connector (505) that is connected to the connecting pipe connector (10a). A first set bolt (502) for abutting and tightening the outer wall of the dextran gel column (10) is threaded on the upper outer wall of the box body (501). The output end of the PLC controller (12) is electrically connected to the input end of the first solenoid valve (506).

8. The sampling device for detecting flavonoids in wine according to claim 1, characterized in that: The intake and exhaust assembly (7) includes an inert gas storage tank (701). A placement seat (710) for placing the inert gas storage tank (701) is fixedly provided on the outer wall of the sampling box (1). A second setter bolt (711) for abutting and tightening the inert gas storage tank (701) is threaded onto the upper side wall of the placement seat (710). An outlet is provided on the upper side of the inert gas storage tank (701), and one end of a spring-type conduit (703) is connected to the outlet. The other end of the spring-type conduit (703) is connected to the ventilation pipe (3) via a main air pipe (702). The main air pipe (702) and the ventilation pipe (3) are integrally formed. The main air pipe (702) is provided with... An air pump (704) is connected to a branch air pipe (706) between the main air pipe (702) on both sides of the air inlet and outlet of the air pump (704). A first three-way valve (705) and a second three-way valve (707) are respectively installed at the connection between the branch air pipe (706) and the main air pipe (702). An exhaust pipe (709) is connected to the main air pipe (702) between the air outlet of the air pump (704) and the second three-way valve (707). A second solenoid valve (708) is installed on the exhaust pipe (709). The output terminal of the PLC controller (12) is electrically connected to the input terminals of the air pump (704), the first three-way valve (705), the second three-way valve (707), and the second solenoid valve (708).

9. The sampling method of the sampling device for detecting flavonoids in wine according to claim 3, characterized in that: Includes the following steps: S1: Remove the outer cover (405) of the sealing plug (4) from the plug body (401), and drive the sampling tube (2) and the ventilation tube (3) to retract into the sampling box (1) by the telescopic drive adjustment component (9); S2: The vacuum cover (11) provided on the lower side of the sampling box (1) and the annular sealing groove (410) opened on the upper side of the plug body (401) are fitted and sealed together. The air intake and exhaust assembly (7) controlled by the PLC controller (12) exhausts the air in the sealed chamber between the vacuum cover (11) and the plug body (401) to form a vacuum environment. S3: In a vacuum environment, the angle adjustment component (8) adjusts the rotation angle of the rotating stage (402) so that the two first perforations (403) and the two second perforations (404) are connected to each other. Then, the telescopic drive adjustment component (9) drives the sampling suction tube (2) and the air tube (3) to extend and pass through the second perforation (404) and the first perforation (403) in sequence into the barrel. The sampling tube structure (6) draws the wine liquid in the barrel into the dextran gel column (10). The dextran gel column (10) realizes the adsorption and separation of flavonoids in the wine liquid. The air intake and exhaust component (7) realizes the protection and pressure maintenance of the barrel by filling it with inert gas. S4: After sampling is completed, the telescopic drive adjustment component (9) drives the sampling suction tube (2) and the ventilation tube (3) to retract into the sampling box (1). Then, the angle adjustment component (8) adjusts the rotation table (402) to rotate, so that the first perforation (403) and the second perforation (404) are separated. Then, the sampling box (1) is separated from the sealing plug (4).

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