A protein blotting device

Abstract

This invention discloses a protein blotting device, belonging to the technical field of immunoblotting equipment. It includes: a base, an incubation tray placement section on the base; an incubation tray placed on the incubation tray placement section, with multiple incubation cells spaced apart on the incubation tray; a liquid addition device for respectively introducing detection solutions into the incubation cells and mounted on the base; a liquid aspiration device for respectively aspirating the detection solutions from the incubation cells and mounted on the base; and a blowing and agitating device for blowing air into the incubation cells to cause the detection solutions in the incubation cells to flow relative to the membrane strip placed in the incubation cells. The blowing and agitating device is mounted on the base and has an air outlet structure, which is located above the incubation tray placement section, with the air outlet of the air outlet structure facing the incubation cells of the incubation tray horizontally placed on the incubation tray placement section. The protein blotting device of this invention helps ensure sufficient contact and reaction between the membrane strip and the detection solution while preventing spillage of the detection solution.

Description

Technical Field

[0001] This invention relates to the field of immunoblotting equipment technology, and more particularly to a protein blotting device. Background Technology

[0002] Immunoblotting, also known as protein blotting, involves transferring proteins onto a membrane and then detecting them using antibodies. Furthermore, immunoblotting offers advantages such as large analytical capacity, high sensitivity, and strong specificity, making it widely used for detecting protein characteristics, expression, and distribution.

[0003] However, in the incubation process using existing immunoblotting instruments, the incubation tray needs to be shaken by the shaking mechanism on the instrument to ensure sufficient contact and reaction between the membrane strip and the detection solution in the incubation tank. During shaking, the vertical reciprocating motion of the shaker, with its difficult-to-control angle, easily leads to spillage of the detection solution from inside and outside the incubation tank, wasting the solution and contaminating the testing environment. Furthermore, as the immunoblotting instrument is used over time, the precision of the shaking mechanism becomes difficult to guarantee, easily resulting in insufficient or excessive shaking. Therefore, there is an urgent need for a protein blotting device that ensures sufficient contact and reaction between the membrane strip and the detection solution while preventing spillage. Summary of the Invention

[0004] The purpose of this invention is to overcome at least one deficiency of the prior art and to provide a protein blotting device that facilitates sufficient contact and reaction between the membrane strip and the detection solution while preventing spillage of the detection solution.

[0005] The technical solution of the present invention to solve the above-mentioned technical problems is as follows: A protein blotting device, comprising:

[0006] A base, wherein the base is provided with an incubation tray placement section for horizontally placing incubation trays;

[0007] An incubation tray is placed on the incubation tray placement part, and the incubation tray is provided with a plurality of incubation tanks at intervals for incubating the membrane strips;

[0008] A liquid adding device is used to input the detection liquid into the incubation tank respectively. The liquid adding device is disposed on one side of the base or installed on the base.

[0009] A liquid aspiration device is used to aspirate the detection liquid from the incubation tank. The liquid aspiration device is disposed on one side of the base or mounted on the base.

[0010] A blowing and agitating device is used to blow air into the incubation tank and cause the detection liquid in the incubation tank to flow relative to the membrane strip placed in the incubation tank. The blowing and agitating device is disposed on one side of the base or mounted on the base. The blowing and agitating device is provided with an air outlet structure, which is disposed above the incubation tray placement part. The air outlet of the air outlet structure faces the incubation tank of the incubation tray placed horizontally on the incubation tray placement part.

[0011] The beneficial effects of this invention are as follows: In this embodiment, by providing a blowing disturbance device, air can be blown into the incubation tank through the air outlet structure on the blowing disturbance device, causing the detection liquid in the incubation tank to flow relative to the membrane strip placed in the incubation tank. This facilitates sufficient contact and reaction between the membrane strip and the detection liquid in the incubation tank, improving the membrane strip incubation effect. Furthermore, the degree of disturbance to the detection liquid in the incubation tank can be flexibly adjusted by controlling the blowing speed and air volume, ensuring suitable disturbance and preventing the detection liquid from overflowing from inside or outside the incubation tank during incubation, thus avoiding waste and contamination of the detection environment. Further, it is beneficial to improve the efficiency of the blowing disturbance device. The device features multiple adjustable speed and volume settings, allowing users to select the appropriate setting based on the required level of agitation of the test solution during incubation. This enables flexible control of the blowing speed and volume, resulting in high precision in agitation control of the test solution. Furthermore, various blowing modes can be employed to achieve different agitation methods for the test solution within the incubation tank, further enhancing the agitation effect. Moreover, the air outlet structure is independent of the incubation tray, ensuring full contact and reaction between the membrane strip and the test solution within the incubation tank without tilting the tray, thus preventing the test solution from overflowing from the incubation tank during incubation.

[0012] In addition, based on the above technical solution, the present invention can be further improved as follows, and can also have the following additional technical features.

[0013] According to one embodiment of the present invention, the protein blotting apparatus further includes:

[0014] The control unit is electrically connected to the liquid adding device, the liquid suction device, and the air blowing disturbance device.

[0015] In this embodiment, by electrically connecting the liquid adding device to the control unit, it is convenient to control the liquid adding device through the control unit to automatically input the test liquid into the incubation tank. By electrically connecting the liquid suction device to the control unit, it is convenient to automatically control the liquid suction device through the control unit to automatically suck out the test liquid from the incubation tank. In addition, by electrically connecting the air blowing disturbance device to the control unit, it is convenient to automatically control the air blowing disturbance device through the control unit to automatically control the air blowing speed and air volume to flexibly adjust the degree of disturbance to the test liquid in the incubation tank, and also to automatically switch the air blowing mode of the air blowing disturbance device.

[0016] According to one embodiment of the present invention, the liquid addition device includes:

[0017] A lateral movement drive mechanism is mounted on the base;

[0018] A lateral movable liquid filling seat is mounted on the lateral movement drive mechanism and is capable of moving laterally relative to the incubation tray above the incubation tray under the drive of the lateral movement drive mechanism;

[0019] The detection solution storage bottle is provided in multiple ways. The base is provided with a bottle body placement part for placing the detection solution storage bottle. The multiple detection solution storage bottles are placed on the bottle body placement part. The multiple detection solution storage bottles are used to store detection solutions. The detection solutions include buffer washing solution, blocking solution, enzyme conjugate, distilled water and colorimetric solution.

[0020] Multiple delivery pumps are provided, each corresponding to one of the multiple storage bottles for the test liquid, and the multiple delivery pumps are installed at intervals on the base;

[0021] Multiple liquid output pipes are provided, each corresponding to one of the multiple delivery pumps. The input end of each liquid output pipe is connected to the delivery pump, and the output end of each liquid output pipe is connected to the horizontal movable liquid filling seat.

[0022] Multiple test liquid input pipes are provided, each corresponding to one of the multiple delivery pumps. The input end of each test liquid input pipe is connected to the test liquid storage bottle, and the output end of each test liquid input pipe is connected to the delivery pump.

[0023] In this embodiment, driven by the lateral movement drive mechanism, the lateral movable liquid filling seat moves laterally above the incubation tray relative to the incubation tray, causing the detection liquid output pipe to move laterally above the incubation tray relative to the incubation tray. By starting the delivery pump, the detection liquid can be sequentially input into the incubation tank.

[0024] According to one embodiment of the present invention, the protein blotting apparatus further includes:

[0025] A membrane strip holder is provided above the incubation tray placement part. The membrane strip holder is provided with multiple placement structures corresponding to the multiple incubation slots of the incubation tray placed on the incubation tray placement part. Each placement structure is provided with a placement part for detachably placing the membrane strip.

[0026] A lifting drive device is installed on the base. The film strip holder is installed on the lifting drive device and can move vertically under the drive of the lifting drive device. The holder and the film strip placed on the holder can extend into the incubation tank under the drive of the lifting drive device.

[0027] In this embodiment, the film strip holder is provided with multiple holding structures, each of which has a holding part for detachably holding the film strip. This facilitates placing the film strip on the holding part and limits the film strip's position, preventing it from flipping over during incubation. Furthermore, by mounting the film strip holder on a lifting drive device, the device can be used to drive the holder to move up and down, making it easy to insert the film strip placed on the holding part into the incubation tank and to remove it from the tank, thus facilitating the assembly and disassembly of the film strip.

[0028] According to one embodiment of the present invention, the blowing disturbance device includes:

[0029] A fan for inputting air, the fan being disposed on one side of the base or mounted on the base;

[0030] An air delivery duct is connected to the air outlet of the fan, and the air outlet structure is connected to the air outlet of the air delivery duct.

[0031] In this embodiment, the air outlet structure is connected to the fan through an air delivery pipeline, which facilitates the input of air to the air outlet structure by the fan and also makes it easier to adjust the air supply by adjusting the fan. In addition, it can improve the flexibility of the fan installation location and facilitate the installation of the fan.

[0032] According to one embodiment of the present invention, the protein blotting apparatus further includes:

[0033] An air heating device is used to heat the air in the air delivery pipeline. The air heating device is installed inside the air delivery pipeline or disposed on the outer periphery of the air delivery pipeline.

[0034] In this embodiment, an air heating device is provided to heat the air in the air delivery pipeline. After the membrane strip incubation is completed, the air in the air delivery pipeline is heated by the air heating device, and then hot air is blown onto the membrane strip to dry it. This dries the membrane strip, making it easier to remove it for the next testing operation. Furthermore, drying the membrane strip by blowing hot air onto it helps to fully dry the membrane strip and improves the efficiency and uniformity of drying.

[0035] According to one embodiment of the present invention, the protein blotting apparatus further includes:

[0036] A hanging rack is provided above the incubation tray placement area;

[0037] The air outlet structure includes:

[0038] Multiple air outlets are provided, and the multiple air outlets are installed at intervals on the suspension frame, with the air outlets of the air outlets facing the incubation tank.

[0039] In this embodiment, multiple air outlets are installed at intervals on the suspension frame, with the air outlets facing the incubation tank. This facilitates airflow through the multiple air outlets towards the incubation tank, which promotes the flow of the detection liquid within the multiple incubation tanks. This, in turn, ensures sufficient contact and reaction between the membrane strip and the detection liquid within the incubation tank, thereby improving the membrane strip incubation effect. Furthermore, blowing air through multiple air outlets towards the incubation tanks also improves the uniformity of airflow through the air outlet structure and enhances the consistency of disturbance to the detection liquid within the multiple incubation tanks.

[0040] According to one embodiment of the present invention, the protein blotting apparatus further includes:

[0041] A transparent light shield is used to cover the outer periphery of the base;

[0042] A second lifting drive device is disposed on the outside of the base. The transparent light shield is mounted on the second lifting drive device and can move up and down in the vertical direction under the drive of the second lifting drive device.

[0043] In this embodiment, by providing a transparent light-shielding cover, the transparent light-shielding cover can be placed on the outer periphery of the base, which is beneficial for blocking light and promoting color development of the film strip during the incubation process. In addition, it is also beneficial for forming a relatively closed space inside the transparent light-shielding cover, reducing or avoiding the influence of external airflow on the space inside the transparent light-shielding cover, and improving the disturbance effect of the air outlet structure on the air blowing disturbance device on the detection liquid in the incubation tank. Furthermore, by installing the transparent light-shielding cover on the lifting drive device two, it is convenient to drive the transparent light-shielding cover to move vertically up and down, which is beneficial for moving the transparent light-shielding cover downward to cover the outer periphery of the base and moving the transparent light-shielding cover upward to expose the base.

[0044] According to one embodiment of the present invention, the air outlet structure is installed inside the transparent light shield, and the air outlet structure can move vertically relative to the incubation tray placement part under the drive of the second lifting drive device.

[0045] In this embodiment, the air outlet structure is installed inside the transparent light shield, which facilitates the installation of the air outlet structure. It also allows the air outlet structure to move synchronously with the transparent light shield in the vertical direction relative to the incubation tray placement part under the drive of the lifting drive device 2. This makes it easy to adjust the distance between the air outlet structure and the incubation tray. The degree of disturbance of the blowing on the test liquid can be adjusted by adjusting the distance between the air outlet structure and the incubation tray, thereby improving the disturbance effect of the air outlet structure on the test liquid in the incubation tank.

[0046] According to one embodiment of the present invention, the protein blotting apparatus further includes:

[0047] Multiple elastic support members are provided at intervals, and the lower ends of the multiple elastic support members are connected to the base in the vertical direction;

[0048] An incubation tray placement base is connected to the upper end of a plurality of elastic support members. The upper side of the incubation tray placement base is provided with an incubation tray placement part. The incubation tray is placed in the incubation tray placement part, and under the blowing action of the blowing disturbance device, the elastic support members generate elastic deformation, and the incubation tray placed on the incubation tray placement part can generate sway.

[0049] In this embodiment, multiple elastic supports are spaced apart, and an incubation tray placement seat is connected to the upper end of the multiple elastic supports. The incubation tray placement seat has an incubation tray placement part for placing the incubation tray. Under the blowing action of the blowing disturbance device, the wind blows onto the incubation tray, and the wind force acts on the incubation tray, causing the elastic supports to undergo elastic deformation. This causes the incubation tray placed on the incubation tray placement part to shake. This allows the air outlet structure to blow air into the incubation tank and cause the detection liquid in the incubation tank to flow relative to the membrane strip placed in the incubation tank. At the same time, the shaking of the incubation tray causes disturbance, which helps to increase the disturbance mode and the disturbance degree, and further improves the disturbance effect of the air outlet structure on the detection liquid in the incubation tank. Attached Figure Description

[0050] To more clearly illustrate the technical solutions in this invention, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0051] Figure 1 This is a schematic diagram of the protein blotting device according to an embodiment of the present invention;

[0052] Figure 2 for Figure 1 A front view of the protein blotting apparatus after it has been properly aligned;

[0053] Figure 3 for Figure 2 Right view of the protein blotting apparatus in the image;

[0054] Figure 4 for Figure 1 A schematic diagram of the protein blotting device after removing the transparent light shield;

[0055] Figure 5 This is a schematic diagram of the lateral movement drive mechanism according to an embodiment of the present invention;

[0056] Figure 6 This is a schematic diagram of the structure of the membrane strip placed on the membrane strip holder according to an embodiment of the present invention;

[0057] Figure 7 This is a schematic diagram of the structure of an incubation tray placed on an incubation tray placement rack according to an embodiment of the present invention;

[0058] Figure 8 This is a schematic diagram of the air outlet structure according to an embodiment of the present invention;

[0059] Figure 9 This is a schematic diagram of the structure of the membrane strip placed on the support structure according to an embodiment of the present invention;

[0060] Figure 10 This is a schematic diagram of the structure of the elastic support member according to an embodiment of the present invention.

[0061] The attached diagram lists the components represented by each number as follows:

[0062] 1. Base; 2. Incubation tray rack; 3. Membrane strip holder; 4. Lateral movement drive mechanism; 5. Support base one; 6. Electric push rod two; 7. Transparent light shield; 8. Air outlet structure; 9. Incubation tray; 10. Support platform one; 11. Support platform two; 12. Control console; 13. Support block; 14. Cleaning tank; 15. Support platform three; 16. Detection liquid storage bottle; 17. Peristaltic pump; 18. Support leg; 20. Mounting base; 21. Elastic support component; 22. Incubation tray rack; 30. Support base two; 31. Electric push rod one; 32. Lifting connecting beam; 3 3. Longitudinal support beam; 34. Support slider; 35. Suspension support beam; 36. Support structure; 37. Membrane strip; 40. Support base three; 41. Nut slider; 42. Guide slider; 43. Lead screw; 44. Guide rod; 45. Connecting seat; 46. Support base four; 47. Electric push rod three; 48. Suspension support plate one; 49. Suspension support plate two; 50. Support base; 51. Vertical support plate; 60. Movable push rod two; 61. Mounting plate one; 62. Mounting plate two; 63. Lifting connecting longitudinal beam; 64. Suspension crossbeam; 70. Inlet pipe; 71. Exhaust pipe; 72. 73. Fixing Bolt 1, 80. Fixing Nut 1, 81. Connecting Support Beam, 82. Inclined Connecting Block 1, 83. Horizontal Support Rod 1, 84. Inclined Connecting Block 2, 85. Horizontal Support Rod 2, 86. Sliding Mount, 87. Air Outlet, 90. Tightening Bolt 5, 101. Incubation Tank, 171. Bottle Placement Groove, 201. Elastic Hose, 211. Horizontal Guide Rail 1, 211. Slider 1, 212. Spring, 213. Slider 2, 214. Tightening Bolt 1, 215. Tightening Bolt 2, 221. Horizontal Guide Rail 2, 222. Sidewall 1, 223. Sidewall 2. 311. Movable push rod 1; 331. Guide slide rail; 341. Tightening bolt 3; 361. Body support; 362. Supporting protrusion; 371. Sample; 401. Bearing; 431. Rotary drive motor; 461. Connecting protrusion; 481. Suction nozzle; 491. Discharge nozzle; 492. Delivery pipe limiting protrusion; 511. Supporting protrusion 1; 512. Supporting protrusion 2; 801. Bolt through hole 1; 851. Mounting protrusion; 852. Connecting protrusion; 2111. Slide groove 1; 2131. Slide groove 2; 2231. Tightening bolt 4; 3621. Support groove. Detailed Implementation

[0063] To make the objectives, technical solutions, and advantages of this application clearer, the embodiments of this application will be described in further detail below with reference to the accompanying drawings.

[0064] To better understand the above-mentioned objectives, features, and advantages of the present invention, the present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments. It should be noted that, unless otherwise specified, the embodiments and features described in these embodiments can be combined with each other.

[0065] Many specific details are set forth in the following description in order to provide a full understanding of the invention. However, the invention may also be practiced in other ways different from those described herein, and therefore the scope of protection of the invention is not limited to the specific embodiments disclosed below.

[0066] This embodiment provides a protein blotting device, such as... Figures 1 to 10 As shown, it includes:

[0067] Base 1, the base 1 is provided with an incubation tray placement part for horizontally placing the incubation tray 9;

[0068] An incubation tray 9 is placed on an incubation tray placement section, and the incubation tray 9 is provided with a plurality of incubation tanks 90 at intervals for incubating the membrane strips 37;

[0069] A liquid adding device is used to input the test liquid into the incubation tank 90 respectively. The liquid adding device is located on one side of the base 1 or installed on the base 1.

[0070] A liquid suction device is used to suction out the test liquid in the incubation tank 90. ​​The liquid suction device is set on one side of the base 1 or installed on the base 1.

[0071] A blowing and agitating device is used to blow air into the incubation tank 90 and cause the detection liquid in the incubation tank 90 to flow relative to the membrane strip 37 placed in the incubation tank 90. ​​The blowing and agitating device is set on one side of the base 1 or installed on the base 1. The blowing and agitating device is provided with an air outlet structure 8, which is set above the incubation tray placement part. The air outlet of the air outlet structure 8 faces the incubation tank 90 of the incubation tray 9 placed horizontally on the incubation tray placement part.

[0072] In this embodiment, as Figures 1 to 10As shown, by incorporating a blowing agitation device, air can be blown into the incubation tank 90 through the air outlet structure 8 on the blowing agitation device, causing the detection liquid in the incubation tank 90 to flow relative to the membrane strip 37 placed in the incubation tank 90. ​​This facilitates sufficient contact and reaction between the membrane strip 37 and the detection liquid in the incubation tank 90, improving the incubation effect of the membrane strip 37. Furthermore, the degree of agitation of the detection liquid in the incubation tank 90 can be flexibly adjusted by controlling the blowing speed and air volume, ensuring suitable agitation and preventing the detection liquid from overflowing from the incubation tank 90 during the incubation process, thus avoiding waste and contamination of the detection environment. Moreover, the blowing agitation device is designed to... The device features multiple adjustable speed and volume settings for wind speed and volume, allowing users to select the appropriate setting based on the required level of agitation of the test solution during incubation. This enables flexible control of the blowing speed and volume, resulting in high precision in controlling the agitation of the test solution. Furthermore, various blowing modes can be employed to achieve different agitation methods for the test solution within the incubation tank 90, further enhancing the agitation effect. Moreover, the air outlet structure 8 and the incubation tray 9 are independent of each other. During the process of ensuring sufficient contact and reaction between the membrane strip 37 and the test solution within the incubation tank 90, the incubation tray 9 remains tilted, which further helps prevent the test solution from overflowing from inside or outside the incubation tank 90 during incubation. It should be noted that the sufficient reaction between the membrane strip 37 and the test solution within the incubation tank 90 specifically refers to the sufficient reaction between the sample 371 placed on the membrane strip 37 and the test solution.

[0073] In one embodiment of the present invention, the protein blotting device further includes a control unit, and a liquid addition device, a liquid aspiration device, and a blower agitation device are electrically connected to the control unit. In this embodiment, by electrically connecting the liquid addition device to the control unit, the control unit can control the liquid addition device to automatically add the detection liquid into the incubation tank 90; by electrically connecting the liquid aspiration device to the control unit, the control unit can automatically control the liquid aspiration device to automatically aspirate the detection liquid from the incubation tank 90; furthermore, by electrically connecting the blower agitation device to the control unit, the control unit can automatically control the blower agitation device to automatically control the blower speed and airflow to flexibly adjust the degree of agitation of the detection liquid in the incubation tank 90, and also facilitate automatic switching of the blower agitation device's blowing mode. It should be noted that the specific structure of the control unit can be found in existing technology and will not be described in detail here.

[0074] In this embodiment, as Figures 1 to 4As shown, a control console 12 is provided on the right side of the support platform 10. The control console 12 is equipped with a touch screen, which is connected to the control unit to facilitate the control operation of the protein blotting device. Furthermore, the bottom of the base 1 is provided with multiple support legs 18, and the base 1 is supported by the multiple support legs 18.

[0075] One embodiment of the present invention, such as Figure 2 , Figure 4 and Figure 5 As shown, the liquid addition device includes:

[0076] The lateral movement drive mechanism 4 is mounted on the base 1;

[0077] A lateral movable liquid filling seat is mounted on a lateral movement drive mechanism 4 and can move laterally above the incubation tray 9 under the drive of the lateral movement drive mechanism 4.

[0078] Multiple detection solution storage bottles 16 are provided. The base 1 is provided with a bottle body placement part for placing the detection solution storage bottles 16. Multiple detection solution storage bottles 16 are placed on the bottle body placement part. The multiple detection solution storage bottles 16 are used to store detection solutions. The detection solutions include buffer washing solution, blocking solution, enzyme conjugate, distilled water and colorimetric solution.

[0079] Multiple delivery pumps are provided, each corresponding to a multiple storage bottle 16 for the test liquid, and the multiple delivery pumps are installed at intervals on the base 1.

[0080] Multiple liquid output pipes are provided for each of the multiple delivery pumps. The input end of each liquid output pipe is connected to a delivery pump, and the output end of each liquid output pipe is connected to a horizontal movable liquid filling seat.

[0081] The test liquid input pipe is provided with multiple pipes corresponding to multiple transfer pumps. The input end of the test liquid input pipe is connected to the test liquid storage bottle 16, and the output end of the test liquid input pipe is connected to the transfer pump.

[0082] In this embodiment, as Figure 2 , Figure 4 and Figure 5 As shown, under the drive of the lateral movement drive mechanism 4, the lateral movable liquid filling seat moves laterally above the incubation tray 9 relative to the incubation tray 9, thereby driving the detection liquid output pipe to move laterally above the incubation tray 9 relative to the incubation tray 9. By starting the delivery pump, the detection liquid can be sequentially input into the incubation tank 90.

[0083] In this embodiment, as Figure 2 , Figure 4 and Figure 5As shown, the lower part of the base 1 is provided with a support platform 10, and the support platform 10 is provided with a bottle placement groove 101. The bottle placement groove 101 serves as a bottle placement part for placing the detection solution storage bottle 16. The left side of the bottle placement groove 101 is open, making it easy to place the detection solution storage bottle 16 into the bottle placement groove 101 from the left side. It should be noted that in this embodiment, the multiple detection solution storage bottles 16 are used to store buffer washing solution, blocking solution, enzyme conjugate, distilled water, and colorimetric solution, respectively. The detection solution storage bottles 16 can also be used to store spare detection solutions.

[0084] In this embodiment, as Figure 2 , Figure 4 and Figure 5 As shown, a second support platform 11 is provided on the base 1. The second support platform 11 is located above the first support platform 10. An incubation tray placement rack 2 is installed on the second support platform 11. The incubation tray placement rack 2 is provided with an incubation tray placement part. Furthermore, a third support platform 15 is provided on the base 1. The third support platform 15 is located behind the second support platform 11. A transverse movement drive mechanism 4 is installed on the third support platform 15.

[0085] In this embodiment, as Figure 2 , Figure 4 and Figure 5 As shown, the lateral movement drive mechanism 4 includes a rotary drive motor 431, a lead screw 43, a lead screw nut slider 41, a guide slider 42, a guide rod 44, and a connecting seat 45. The lead screw nut slider 41 is threadedly connected to the outside of the lead screw 43, and the lead screw 43 is connected to the rotation shaft of the rotary drive motor 431. Specifically, the rotary drive motor 431 is mounted on a support base 3 40. Bearings 401 are respectively mounted on both ends of the lead screw 43 and supported by two support bases 3 40. The bearings 401 mounted on both ends of the lead screw 43 are mounted on the support bases 3 40 used to support the lead screw 43. The guide rod 44 is arranged parallel to the lead screw 43, and both ends of the guide rod 44 are respectively fixed on two support bases 3 40. The guide slider 42 is slidably mounted on the outside of the guide rod 44, facing the lead screw nut slider 41. The connecting seat 45 is connected to the lead screw nut slider 41 and the guide slider 42. In this embodiment, the support bases 3 40 are all mounted on the support platform 3 15.

[0086] Furthermore, in this embodiment, the rotation of the rotary drive motor 431 controls the rotation of the lead screw 43, thereby controlling the movement position of the lateral movable liquid-adding seat and sequentially inputting the test liquid into the incubation tank 90. ​​The rotary drive motor 431 is electrically connected to the control unit. Under the rotational drive of the rotary drive motor 431, the lead screw nut slider 41 moves along the length direction of the lead screw 43, and the guide slider 42 moves synchronously with the lead screw nut slider 41 along the length direction of the guide rod 44, thereby driving the connecting seat 45 to move laterally. Furthermore, the lateral movement drive mechanism 4 in this embodiment can also adopt other displacement drive devices.

[0087] Furthermore, such as Figure 2 , Figure 4 and Figure 5 As shown, the transverse movable liquid filling seat in this embodiment includes a second suspension support plate 49. The rear end of the second suspension support plate 49 is fixed to the connecting seat 45 by bolts. The front end of the second suspension support plate 49 is provided with a plurality of mounting through holes 1 at intervals. A plurality of liquid outlets 491 are installed in the plurality of mounting through holes 1. The upper end of the liquid outlets 491 is respectively used to connect to the detection liquid output pipe that can move with the second suspension support plate 49. The lower end of the liquid outlets 491 is provided with an outlet. Under the rotation drive of the rotary drive motor 431, the plurality of liquid outlets 491 can move sequentially above the incubation tank 90 in the transverse direction. Furthermore, the front end of the second suspension support plate 49 is provided with a plurality of delivery pipe limiting protrusions 492 at intervals. The output end of the detection liquid output pipe can be snapped between two adjacent delivery pipe limiting protrusions 492 to facilitate limiting the output end of the detection liquid output pipe.

[0088] Furthermore, such as Figure 2 , Figure 4 and Figure 5 As shown, the pump in this embodiment is specifically a peristaltic pump 17. The peristaltic pump 17 is mounted on the base 1. An elastic hose 171, which connects the detection liquid input pipe and the detection liquid output pipe, is mounted on a rotating extrusion head on the peristaltic pump 17. During the rotation of the rotating extrusion head, the hose 171 is intermittently extruded to achieve liquid input. Furthermore, the peristaltic pump 17 is connected to a control unit, which controls the peristaltic pump 17. It should be noted that the detection liquid input pipe and the detection liquid output pipe in this embodiment are not shown in the figure. The specific arrangement of the detection liquid input pipe and the detection liquid output pipe can be varied, and the liquid addition device can also be an existing liquid addition device used for protein blot instruments. In addition, the specific structure and working principle of the peristaltic pump 17 can be referred to the prior art in this field, and will not be described in detail here.

[0089] Furthermore, such as Figure 2 , Figure 4 and Figure 5As shown, the liquid suction device in this embodiment includes a lifting drive device, a liquid suction pump, a liquid suction pipe, a liquid suction nozzle 481, and a suspension support plate 48. The lifting device is installed on the base 1. The suspension support plate 48 has a bent structure. The rear end of the suspension support plate 48 is fixed to the lifting device by bolts. The front end of the suspension support plate 48 extends forward and bends downward to form an extension end. The extension end of the suspension support plate 48 is provided with a second mounting through hole for installing the liquid suction nozzle 481. The liquid suction nozzle 481 is installed in the second mounting through hole and extends downward. The upper end of the liquid suction nozzle 481 is connected to the liquid suction pipe that can move with the suspension support plate 48. The lower end of the liquid suction nozzle 481 is provided with a liquid suction port. The liquid suction pump is connected to the liquid suction pipe. The output end of the liquid suction pipe is used to connect to a waste liquid tank. The waste liquid tank can be set on one side of the base 1 for storing waste liquid. Furthermore, the lifting drive device is an electric push rod 3 47, which is mounted on the support base 46. The front side of the support base 46 is provided with a connecting protrusion 461, which is mounted on the rear end of the connecting seat 45 by bolts. The rear end of the suspension support plate 1 48 is connected to the movable push rod on the electric push rod 3 47.

[0090] Furthermore, driven by the rotary drive motor 431, the aspiration nozzle 481 can move sequentially to the top of the incubation tank 90 in the lateral direction; and driven by the lifting of the electric push rod 47, the aspiration nozzle 481 can move up and down in the vertical direction. In addition, the electric push rod 47 in this embodiment is connected to the control unit, which controls the electric push rod 47. The aspiration pump is also connected to the control unit. It should be noted that the aspiration pump and aspiration tube in this embodiment are not shown in the figures. The specific configuration of the aspiration pump and aspiration tube can be varied. Furthermore, the aspiration device can also be an existing aspiration device used in Western blot instruments.

[0091] Furthermore, such as Figures 1 to 4 As shown, a support block 13 is provided at the right end of the support platform 2 11. The support block 13 is located on the right side of the incubation tray placement rack 2. A cleaning tank 14 is installed at the upper end of the support block 13 to facilitate cleaning of the suction nozzle 481. In addition, the suction nozzle 481 can move above the cleaning tank 14 and extend into the bottom of the cleaning tank 14 to suction the liquid in the cleaning tank 14 under the drive of the electric push rod 3 47.

[0092] One embodiment of the present invention, such as Figure 4 and Figure 6 As shown, the protein blotting device also includes:

[0093] The film strip holder 3 is used to be set above the incubation tray placement part. The film strip holder 3 is provided with multiple placement structures 36 corresponding to the multiple incubation slots 90 of the incubation tray 9 placed on the incubation tray placement part. Each placement structure 36 is provided with a placement part for detachable placement of film strip 37.

[0094] A lifting drive device 1 is installed on the base 1. The film strip holder 3 is installed on the lifting drive device 1 and can move vertically under the drive of the lifting drive device 1. The holder and the film strip 37 placed on the holder can extend into the incubation tank 90 under the drive of the lifting drive device 1.

[0095] In this embodiment, as Figure 4 and Figure 6 As shown, the film strip holder 3 is provided with multiple holding structures 36, which facilitates the placement of the film strip 37 on the holding part and can limit the film strip 37 through the holding part to prevent the film strip 37 from flipping up during the incubation process. Furthermore, by installing the film strip holder 3 on the lifting drive device, it is convenient to drive the film strip holder 3 to lift and lower through the lifting drive device, thereby facilitating the insertion and removal of the film strip 37 placed on the holding part into the incubation tank 90 and the removal of the film strip 37 placed on the holding part from the incubation tank 90, which facilitates the assembly and disassembly of the film strip 37.

[0096] In this embodiment, as Figure 4 and Figure 6 As shown, the membrane strip holder 3 in this embodiment includes: longitudinal support beams 33, support sliders 34, and suspension support beams 35. Two longitudinal support beams 33 are spaced parallel to each other in the transverse direction and extend horizontally in the longitudinal direction. Guide rails 331 are connected to each of the two longitudinal support beams 33. Two support sliders 34 are provided and slidably mounted on the guide rails 331 respectively. The suspension support beams 35 are connected between the two support sliders 34. Multiple placement structures 36 are detachably connected to the suspension support beams 35 at intervals in the transverse direction. Furthermore, in this embodiment, two suspension support beams 35 are provided, and the two suspension support beams 35 are spaced parallel to each other in the longitudinal direction.

[0097] In this embodiment, as Figure 3 and Figure 6 As shown, the membrane strip holder 3 also includes: a locking component 1, which is provided in multiple forms. Each locking component 1 is connected to a support slider 34, and each locking component 1 can lock the support slider 34 onto the guide rail 331. Further, in this embodiment, the locking component 1 is specifically a tightening bolt 341. Each of the two support sliders 34 is provided with a threaded hole 1, which communicates with the slide groove inside the support slider 34. The tightening bolt 341 is threadedly connected to the threaded hole 1, and the screw-in end of the tightening bolt 341 can stop and lock the support slider 34 with the guide rail 331. In addition, by loosening the tightening bolt 341, the two support sliders 34 and the suspension support beam 35 can be taken out upwards.

[0098] In this embodiment, as Figure 6 and Figure 9As shown, the placement structure 36 includes: a placement body 361, the upper end of which is provided with a hook structure for detachable suspension on the support body, the hook structure being detachably suspended on the suspension support beam 35, and the lower end of the placement body 361 is provided with a placement part.

[0099] In this embodiment, as Figure 9 As shown, the support body 361 has a bent structure, and a U-shaped snap-fit ​​groove is formed on the inner side of the support body 361. The cross-section of the suspension support beam 35 in the width direction is rectangular. The suspension support beam 35 snaps into the snap-fit ​​groove in the support body 361. A horizontally extending protruding support protrusion 362 is connected to the lower end of the support body 361, and a support groove 3621 is formed on the support protrusion 362. Furthermore, the support body 361 is made of an elastic material. The hook structure is suspended on the suspension support beam 35. The support body 361 undergoes elastic deformation and compresses the suspension support beam 35. In addition, the support body 361 can also be configured with other structures, and the support part can also be configured with other structures to facilitate the placement of the membrane strip 37.

[0100] In this embodiment, as Figure 9 As shown, the resting part is a resting groove 3621, which is horizontally arranged at the lower end of the resting structure 36 and has three open sides; this facilitates the insertion of the membrane strip 37 into the resting groove 3621, and also allows both ends of the membrane strip 37 to pass through the resting groove 3621, making it easy to adjust the resting position of the membrane strip 37 and the resting groove 3621 so that the membrane strip 37 is horizontally resting in the resting groove 3621; in addition, it also facilitates quick assembly and disassembly of the membrane strip 37.

[0101] In this embodiment, as Figure 4 and Figure 9 As shown, in this embodiment, the rear ends of the two longitudinal support beams 33 are connected to a lifting connecting beam 32, which connects the two longitudinal support beams 33 into one unit; furthermore, in this embodiment, the longitudinal support beams 33 are of uniform length and plate-like structure, and the two suspension support beams 35 are of bent structure. The left and right ends of the two suspension support beams 35 are respectively provided with horizontal connecting sections, and the middle of the suspension support beams 35 is provided with a horizontally arranged suspension section. The left and right ends of the suspension section are respectively connected to the horizontal connecting section through vertical connecting sections, and the suspension section is located below the horizontal connecting section.

[0102] It needs to be stated that yes, such as Figure 6 and Figure 9As shown, the membrane strip 37 has a long strip structure, and multiple samples 371 are spaced apart on the membrane strip 37. When the membrane strip 37 is placed horizontally in two oppositely arranged placement grooves 3621, the samples 371 spaced apart on the membrane strip 37 are staggered from the placement grooves 3621. In addition, the depth of the vertical groove of the placement groove 3621 is slightly greater than the thickness of the membrane strip 37.

[0103] In this embodiment, as Figure 4 As shown, the lifting drive device in this embodiment includes: an electric push rod 31 for fixed installation, a movable push rod 311 provided on the electric push rod extending vertically upward to form an extension end, and a membrane strip holder 3 horizontally connected to the extension end of the movable push rod 311.

[0104] In this embodiment, as Figure 4 As shown, in this embodiment, there are two electric push rods 31, which are respectively mounted on support bases 30. The two support bases 30 are mounted on the left and right ends of the rear side of the base 1. The left and right ends of the lifting connecting beam 32 are connected to the movable push rods 311 of the two electric push rods 31. Furthermore, in this embodiment, the electric push rods 31 are connected to the control unit, which controls the electric push rods 31. Furthermore, the lifting drive device can also adopt other lifting devices to facilitate the lifting drive of the film strip holder 3 to adjust the height position of the film strip holder 3. In addition, the film strip holder 3 can also be configured with other structures.

[0105] In one embodiment of the present invention, the blowing disturbance device includes: a fan for inputting air, the fan being disposed on one side of the base 1 or mounted on the base 1; an air delivery pipeline connected to the air outlet end of the fan, and an air outlet structure 8 connected to the air outlet end of the air delivery pipeline.

[0106] In this embodiment, the air outlet structure 8 is connected to the fan via an air delivery pipeline, facilitating the input of air to the air outlet structure 8 through the fan and enabling the adjustment of air supply by regulating the fan. Additionally, this improves the flexibility of the fan's installation location, making installation easier. Furthermore, in this embodiment, the fan is connected to a control unit, which controls the fan's operating mode to achieve different blowing modes. The control unit can automatically adjust the airflow size, duration, and duration, enabling various blowing modes such as intermittent airflow and gradual increases in airflow force.

[0107] In one embodiment of the present invention, the protein blotting apparatus further includes an air heating device for heating the air in the air delivery pipeline, the air heating device being installed inside the air delivery pipeline. Alternatively, the air heating device may be disposed on the outer periphery of the air delivery pipeline.

[0108] In this embodiment, an air heating device is provided to heat the air in the air delivery pipeline. After the membrane strip 37 has been incubated, the air in the air delivery pipeline is heated by the air heating device, and then hot air is blown onto the membrane strip 37 to dry it, thus drying the membrane strip 37. This facilitates the removal of the dried membrane strip 37 for the next testing operation. Furthermore, drying the membrane strip 37 by blowing hot air helps to ensure thorough drying and improves drying efficiency and uniformity. In this embodiment, the air heating device is connected to a control unit, which controls the air heating device. The air heating device in this embodiment uses a heating wire installed inside the air delivery pipeline. The air heating device is not illustrated in this embodiment. Alternatively, other suitable electric heating devices in the prior art can also be used as the air heating device.

[0109] One embodiment of the present invention, such as Figure 4 and Figure 8 As shown, the protein blotting device also includes: a suspension frame, which is set above the incubation tray placement section; the air outlet structure 8 includes: multiple air outlets 86, which are spaced apart and installed on the suspension frame, with the air outlets of the air outlets 86 facing the incubation tank 90.

[0110] In this embodiment, as Figure 4 and Figure 8 As shown, by installing multiple air outlets 86 at intervals on the suspension frame, with the air outlets of the air outlets 86 facing the incubation tank 90, it is convenient to blow air into the incubation tank 90 through the multiple air outlets 86. This facilitates the flow of the detection liquid in the multiple incubation tanks 90, which in turn facilitates full contact and reaction between the membrane strip 37 and the detection liquid in the incubation tank 90, thereby improving the incubation effect of the membrane strip 37. Furthermore, blowing air into the incubation tank 90 through the multiple air outlets 86 can also improve the uniformity of the air blowing from the air outlet structure 8 into the incubation tank 90, which is beneficial to improving the consistency of the disturbance of the detection liquid in the multiple incubation tanks 90.

[0111] In this embodiment, as Figure 4 and Figure 8 As shown, multiple air outlets 86 are respectively used to connect to the fan through air delivery pipes, and the multiple air outlets 86 are installed at intervals on the suspension bracket. Furthermore, the air outlets 86 are detachably installed on the suspension bracket, and the direction of the air outlet of the air outlet 86 is adjustable; in addition, the air delivery pipes and the fan in this embodiment are not shown.

[0112] Furthermore, such as Figure 4 and Figure 8As shown, the air outlet structure 8 also includes: a sliding mounting base 85 and a second locking member. Multiple sliding mounting bases 85 are provided corresponding to multiple air outlets 86. The sliding mounting bases 85 are slidably mounted on the transverse support rod, and the air outlets 86 are mounted on the sliding mounting bases 85. Multiple second locking members are provided corresponding to multiple sliding mounting bases 85. The second locking member is connected to the sliding mounting base 85 and can lock the sliding mounting base 85 onto the transverse support rod. In this embodiment, the spacing between two adjacent sliding mounting bases 85 is adjusted in the transverse direction, thereby facilitating the adjustment of the spacing between the multiple air outlets 86 in the transverse direction. Furthermore, locking the sliding mounting bases 85 with the second locking member helps to fix the positions of the sliding mounting bases 85 and the air outlets 86.

[0113] Furthermore, such as Figure 4 and Figure 8 As shown, the air inlet end of the air outlet 86 is provided with a connecting part for connecting to the air supply pipe, and the connecting part is provided with an air inlet; furthermore, one side of the sliding mounting base 85 is provided with a mounting protrusion 851, and the mounting protrusion 851 is provided with a mounting hole, and the air outlet 86 is installed in the mounting hole. Specifically, the air outlet 86 is installed with an interference fit with the mounting hole; in addition, the air outlet 86 can also be installed on the sliding mounting base 85 by other installation methods.

[0114] Furthermore, such as Figure 4 and Figure 8 As shown, the second locking component is a tightening bolt 87. A connecting protrusion 852 is provided on one side of the sliding mounting base 85. The connecting protrusion 852 is provided with a threaded through hole that communicates with the mounting port. The tightening bolt 87 is threadedly connected in the threaded through hole. By tightening the tightening bolt 87, the screw-in end of the tightening bolt 87 stops against the transverse support rod and locks the sliding mounting base 85. By loosening the tightening bolt 87, the sliding mounting base 85 can be adjusted by sliding along the transverse support rod to adjust the position of the sliding mounting base 85. The orientation of the air outlet 86 can also be adjusted by rotating the sliding mounting base 85 circumferentially.

[0115] In this embodiment, as Figure 4 and Figure 8 As shown, the suspension bracket includes:

[0116] Support structure;

[0117] A horizontal support rod 82 is connected to the support structure and extends horizontally in the lateral direction. Multiple air outlets 86 are slidably installed on the horizontal support rod 82 at intervals.

[0118] The second horizontal support rod 84 is connected to the support structure and is set parallel to and spaced apart from the first horizontal support rod 82. Multiple air outlets 86 are slidably installed on the second horizontal support rod 84 at intervals.

[0119] In this embodiment, as Figures 1 to 4 As shown, this embodiment includes a transparent light-shielding cover 7, which is mounted on the electric push rod 6. The support structure is specifically a connecting support beam 80, which is horizontally mounted on the top plate of the transparent light-shielding cover 7. A horizontal support rod 82 is connected to the lower end of the connecting support beam 80 via an inclined connecting block 81, and the inclined connecting block 81 is connected to the middle of the horizontal support rod 82. The left and right ends of the horizontal support rod 82 extend horizontally to form free ends. In addition, a horizontal support rod 84 is connected to the lower end of the connecting support beam 80 via an inclined connecting block 83, and the inclined connecting block 83 is connected to the middle of the horizontal support rod 84. The left and right ends of the horizontal support rod 84 extend horizontally to form free ends.

[0120] Furthermore, such as Figures 1 to 4 As shown, in this embodiment, the connecting support beam 80 is provided with multiple bolt through holes 801 spaced apart, and the transparent light shield 7 is provided with multiple bolt through holes 801 corresponding to the bolt through holes 801. In this embodiment, fixing bolts 72 pass through the corresponding bolt through holes 801 and bolt through holes 801 and are fixed by fixing nuts 73. Furthermore, the hanging bracket can also be configured with other structures to facilitate the installation of multiple air outlets 86.

[0121] Furthermore, such as Figures 1 to 4 As shown, in this embodiment, the air inlet pipe 70 in the air supply pipeline used to supply air to the multiple air outlets 86 installed inside the transparent light shield 7 passes through the top plate of the transparent light shield 7. Additionally, in this embodiment, the exhaust pipe 71 used for exhausting air passes through the rear side wall of the transparent light shield 7, facilitating the discharge and treatment of gas inside the transparent light shield 7 through the exhaust pipe 71. It should be noted that the air inlet pipe 70 and exhaust pipe 71 in this embodiment are flexible hoses and can move with the transparent light shield 7. Furthermore, the air inlet pipe 70 and exhaust pipe 71 are not fully illustrated; there are various ways to arrange and connect the fan, air inlet pipe 70, and exhaust pipe 71, and existing technologies in the field can be referenced, which will not be elaborated here.

[0122] One embodiment of the present invention, such as Figures 1 to 4 As shown, the protein blotting device also includes:

[0123] A transparent light shield 7 is used to cover the outer periphery of the base 1;

[0124] The second lifting drive device is located on the outside of the base 1. The transparent light shield 7 is installed on the second lifting drive device and can move up and down in the vertical direction under the drive of the second lifting drive device.

[0125] In this embodiment, as Figures 1 to 4As shown, by providing a transparent light-shielding cover 7, the transparent light-shielding cover 7 can be placed on the outer periphery of the base 1, which is beneficial for shielding the film strip 37 from light and promoting color development during the incubation process of the film strip 37. In addition, it is also beneficial for forming a relatively closed space inside the transparent light-shielding cover 7, reducing or avoiding the influence of external airflow on the space inside the transparent light-shielding cover 7, and improving the disturbance effect of the air outlet structure 8 provided on the blowing disturbance device on the detection liquid in the incubation tank 90. ​​Furthermore, by installing the transparent light-shielding cover 7 on the lifting drive device 2, it is convenient to drive the transparent light-shielding cover 7 to move vertically, which is beneficial for moving the transparent light-shielding cover 7 downward to cover the outer periphery of the base 1 and for moving the transparent light-shielding cover 7 upward to expose the base 1.

[0126] In this embodiment, as Figures 1 to 4 As shown, the second lifting drive device includes: an electric push rod 6 for fixed installation, and a movable push rod 60 on the electric push rod 6 extending vertically upward to form an extension end; in this embodiment, the electric push rod 6 is installed on the support base 5, which is fixedly installed on the rear side of the base 1. The support base 5 includes a support base 50 and a vertical support plate 51 vertically connected to the support base 50. The front end of the vertical support plate 51 is connected to a support protrusion 511 and a support protrusion 512. The middle portions of block 1 511 and support protrusion 2 512 are respectively provided with vertical through slots; furthermore, mounting plates 1 61 and 2 62 are respectively connected to the outer side wall of electric push rod 2 6 corresponding to support protrusion 1 511 and support protrusion 2 512. Mounting plate 1 61 is fixedly connected to support protrusion 1 511 by bolts, and mounting plate 2 62 is fixedly connected to support protrusion 2 512 by bolts. The body of electric push rod 2 6 passes through the vertical through slots provided on support protrusion 1 511 and support protrusion 2 512. Furthermore, support base 1 5 can also be configured with other structures, and electric push rod 2 6 can also be installed on support base 1 5 by other installation methods.

[0127] Furthermore, such as Figures 1 to 4 As shown, in this embodiment, a lifting connecting beam 63 is connected to the upper end of the movable push rod 60. The lifting connecting beam 63 extends forward to form an extension end, and a suspension beam 64 is connected to the extension end of the lifting connecting beam 63. Multiple bolt through holes 3 are spaced apart on the suspension beam 64. The top plate of the transparent light shield 7 corresponds to multiple bolt through holes 4 on the suspension beam 64. In this embodiment, fixing bolts 72 pass through the corresponding bolt through holes 3 and 4 and are fixed by fixing nuts 73, thus suspending the transparent light shield 7 on the suspension beam 64. Furthermore, the transparent light shield 7 has a hollow cuboid structure. Furthermore, the electric push rod 6 is connected to a control unit, which controls the electric push rod 6. Alternatively, other lifting devices can be used for the lifting drive device 2.

[0128] One embodiment of the present invention, such as Figure 1 , Figure 4 and Figure 7 As shown, the air outlet structure 8 is installed inside the transparent light shield 7. Driven by the second lifting drive device, the air outlet structure 8 moves vertically relative to the incubation tray placement part along with the transparent light shield 7. In this embodiment, by installing the air outlet structure 8 inside the transparent light shield 7, the air outlet structure 8 can move synchronously with the transparent light shield 7 relative to the incubation tray placement part in the vertical direction under the drive of the second lifting drive device. This facilitates adjustment of the distance between the air outlet structure 8 and the incubation tray 9. Adjusting this distance allows for adjustment of the degree of disturbance of the blowing air to the detection liquid, thereby improving the disturbance effect of the air outlet structure 8 on the detection liquid within the incubation tank 90.

[0129] One embodiment of the present invention, such as Figure 1 , Figure 4 and Figure 7 As shown, the protein blotting device also includes:

[0130] Multiple elastic support members 21 are provided at intervals, and the lower ends of the multiple elastic support members 21 are connected to the base 1 in the vertical direction.

[0131] An incubation tray placement seat 22 is connected to the upper end of a plurality of elastic support members 21. An incubation tray placement part is provided on the upper side of the incubation tray placement seat 22. An incubation tray 9 is placed in the incubation tray placement part, and under the blowing action of the blowing disturbance device, the elastic support members 21 undergo elastic deformation, and the incubation tray 9 placed on the incubation tray placement part can shake.

[0132] In this embodiment, as Figure 1 , Figure 4 and Figure 7 As shown, multiple elastic support members 21 are spaced apart, and an incubation tray placement seat 22 is connected to the upper end of the multiple elastic support members 21. The incubation tray placement seat 22 is provided with an incubation tray placement part for placing the incubation tray 9. Under the blowing action of the blowing disturbance device, the wind blows onto the incubation tray 9, and the wind force acts on the incubation tray 9, causing the elastic support members 21 to undergo elastic deformation. This causes the incubation tray 9 placed on the incubation tray placement part to shake. This allows the air outlet structure 8 to blow air into the incubation tank 90 and cause the detection liquid in the incubation tank 90 to flow relative to the membrane strip 37 placed in the incubation tank 90. ​​At the same time, the shaking of the incubation tray 9 causes disturbance, which helps to increase the disturbance mode and the disturbance degree, and further improves the disturbance effect of the air outlet structure 8 on the detection liquid in the incubation tank 90.

[0133] Furthermore, such as Figure 2 and Figure 7As shown, the protein blotting device also includes a mounting base 20, which is mounted on a support platform 11. An incubation tray placement seat 22 and multiple elastic support members 21 constitute an incubation tray placement frame 2. Multiple elastic support members 21 are vertically connected at intervals between the mounting base 20 and the incubation tray placement seat 22. An incubation tray placement groove is provided on the upper side of the incubation tray placement seat 22.

[0134] In this embodiment, such as Figure 2 and Figure 7 As shown, the upper side of the mounting base 20 is provided with multiple horizontal guide rails 201 at intervals, and the lower side of the incubation tray placement seat 22 is provided with multiple horizontal guide rails 221 directly opposite the multiple horizontal guide rails 201; the elastic support member 21 includes a slider 211, a slider 213 and a spring 212. The slider 211 is slidably mounted on the horizontal guide rail 201; the slider 213 is slidably mounted on the horizontal guide rail 221; the spring 212 is connected between the slider 211 and the slider 213.

[0135] In this embodiment, as Figure 2 , Figure 7 and Figure 10 As shown, slider 211 is slidably mounted on transverse guide rail 201, and slider 213 is slidably mounted on transverse guide rail 221. This facilitates the adjustment of the position of the elastic support 21, thereby facilitating the adjustment of the spacing between multiple elastic support 21s. It also facilitates the adjustment of the degree of shaking of the incubation tray placement seat 22 under the disturbance of the disturbance driving device, so that the flow of the detection liquid relative to the membrane strip 37 placed in the incubation tank 90 is suitable.

[0136] In this embodiment, as Figure 2 , Figure 7 and Figure 10 As shown, the mounting base 20 is horizontally mounted on the support platform 11, and the transverse guide slide rail 201 is vertically connected to the upper side of the mounting base 20. In addition, in this embodiment, there are three transverse guide slide rails 201, which are spaced apart in the longitudinal direction. The slider 211 is slidably mounted on the transverse guide slide rail 201, and the transverse guide slide rail 201 extends into the slide groove 2111 provided on the slider 211.

[0137] Furthermore, such as Figure 2 , Figure 7 and Figure 10As shown, in this embodiment, the incubation tray placement base 22 is horizontally mounted on the upper end of multiple elastic support members 21, and the second transverse guide slide rail 221 is vertically connected to the lower side of the incubation tray placement base 22. In this embodiment, the second transverse guide slide rail 221 is provided opposite to the three first transverse guide slide rails 201 respectively; the second slider 213 is slidably mounted on the second transverse guide slide rail 221, and the second transverse guide slide rail 221 extends into the second groove 2131 provided on the second slider 213. Furthermore, the elastic support member 21 in this embodiment can also be other elastic support components.

[0138] In this embodiment, as Figure 2 , Figure 7 and Figure 10 As shown, the incubation tray placement rack 2 also includes: a first tightening component and a second tightening component. The first tightening component and the second tightening component are respectively provided with multiple elastic support components 21. The first tightening component is connected to the first slider 211 and locks the first slider 211 onto the first transverse guide slide rail 201. The second tightening component is connected to the second slider 213 and locks the second slider 213 onto the second transverse guide slide rail 221.

[0139] Furthermore, the tightening component is a tightening bolt 214. A threaded hole 2 is provided on one side of the slider 211. The tightening bolt 214 is threadedly connected to the threaded hole 2, and the screw-in end of the tightening bolt 214 extends into the slide groove 2111. Tightening the tightening bolt 214 causes the screw-in end of the tightening bolt 214 to stop against the transverse guide rail 201, locking the slider 211 onto the transverse guide rail 201. Loosening the tightening bolt 214 allows the slider 211 to move along the transverse guide rail 201. 01 slides along the length direction; the second tightening part is the second tightening bolt 215, and the second slider 213 has a threaded hole 3 on one side. The second tightening bolt 215 is threadedly connected to the threaded hole 3 and the screw-in end of the second tightening bolt 215 extends into the slide groove 2131. Tightening the second tightening bolt 215 locks the second slider 213 onto the transverse guide slide rail 221. Loosening the second tightening bolt 215 allows the second slider 213 to slide along the length direction of the transverse guide slide rail 221.

[0140] In this embodiment, as Figure 7As shown, the incubation tray placement base 22 has an incubation tray placement groove on its upper side, which extends in the transverse direction. The incubation tray placement groove has a first baffle 222 at its rear end in the longitudinal direction and a second baffle 223 at its front end in the longitudinal direction. When the rear end of the incubation tray 9 is placed against the first baffle 222 and the incubation tray 9 is placed in the incubation tray placement groove, there is a gap between the front end of the incubation tray 9 and the second baffle 223. The incubation tray placement rack 2 also includes a tightening member three, which is provided in multiple ways. The second baffle 223 has multiple threaded holes spaced along its length direction. The multiple tightening members three are threadedly connected to the multiple threaded holes respectively. The screw-in end of the tightening member three can extend into the incubation tray placement groove and stop against the incubation tray 9.

[0141] In addition, such as Figure 7 As shown, in this embodiment, the tightening component three is the tightening bolt four 2231, and the flange two 223 is provided with a threaded hole, specifically the threaded hole four. The tightening bolt four 2231 is threadedly connected to the threaded hole four, and the screw-in end of the tightening bolt four 2231 extends into the incubation tray placement groove. Tightening the tightening bolt four 2231, the tightening bolt four 2231 and the incubation tray 9 stop lock the incubation tray 9 in the incubation tray placement groove.

[0142] Furthermore, such as Figure 1 , Figure 7 As shown in the illustration, three incubation trays 9 are placed in the incubation tray placement slot in this embodiment. The incubation trays 9 are locked in place by tightening bolts 2231. Alternatively, two or four incubation trays 9 can be placed as needed. It should be noted that other structures of the protein blotting device can be found in existing protein blotting detectors in the art, and will not be described in detail here.

[0143] In addition to the technical solutions disclosed in this embodiment, other components of the rotary drive motor 431, electric push rod, membrane strip 37, control unit, protein blotting device, and their working principles in this invention can be referred to conventional technical solutions in this technical field. However, these conventional technical solutions are not the focus of this invention, and will not be described in detail here.

[0144] In this invention, the term "multiple" refers to two or more unless otherwise explicitly defined. The terms "install," "connect," "link," and "fix" should be interpreted broadly. For example, "connect" can be a fixed connection, a detachable connection, or an integral connection; "link" can be a direct connection or an indirect connection through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0145] In the description of this invention, it should be understood that the terms "upper," "lower," "front," "rear," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or unit referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this application.

[0146] In the description of this specification, the terms "one embodiment," "some embodiments," "specific embodiment," etc., refer to a specific feature, structure, material, or characteristic described in connection with that embodiment or example, which is included in at least one embodiment or example of this application. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0147] The above are merely preferred embodiments of this application and are not intended to limit this application. Various modifications and variations can be made to this application by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the protection scope of this application.

Claims

1. A protein blotting device, characterized in that, include: A base, wherein the base is provided with an incubation tray placement section for horizontally placing incubation trays; An incubation tray is placed on the incubation tray placement part, and the incubation tray is provided with a plurality of incubation tanks at intervals for incubating the membrane strips; A liquid adding device is used to input the detection liquid into the incubation tank respectively. The liquid adding device is disposed on one side of the base or installed on the base. A liquid aspiration device is used to aspirate the detection liquid from the incubation tank. The liquid aspiration device is disposed on one side of the base or mounted on the base. A blowing and agitating device is used to blow air into the incubation tank and cause the detection liquid in the incubation tank to flow relative to the membrane strip placed in the incubation tank. The blowing and agitating device is disposed on one side of the base or mounted on the base. The blowing and agitating device is provided with an air outlet structure. The air outlet structure is disposed above the incubation tray placement part, and the air outlet of the air outlet structure faces the incubation tank of the incubation tray placed horizontally on the incubation tray placement part. The blowing disturbance device includes: A fan for inputting air, the fan being disposed on one side of the base or mounted on the base; An air delivery duct is connected to the air outlet of the fan, and the air outlet structure is connected to the air outlet of the air delivery duct. The protein blotting device further includes: An air heating device is used to heat the air in the air delivery pipeline. The air heating device is installed inside the air delivery pipeline or disposed on the outer periphery of the air delivery pipeline. The protein blotting device further includes: Multiple elastic support members are provided at intervals, and the lower ends of the multiple elastic support members are connected to the base in the vertical direction; An incubation tray placement base is connected to the upper end of a plurality of elastic support members. The upper side of the incubation tray placement base is provided with an incubation tray placement part. The incubation tray is placed in the incubation tray placement part, and under the blowing action of the blowing disturbance device, the elastic support members generate elastic deformation, and the incubation tray placed on the incubation tray placement part can generate sway.

2. The protein blotting device according to claim 1, characterized in that, Also includes: The control unit is electrically connected to the liquid adding device, the liquid suction device, and the air blowing disturbance device.

3. The protein blotting device according to claim 1, characterized in that, The liquid addition device includes: A lateral movement drive mechanism is mounted on the base; A lateral movable liquid filling seat is mounted on the lateral movement drive mechanism and is capable of moving laterally relative to the incubation tray above the incubation tray under the drive of the lateral movement drive mechanism; The detection solution storage bottle is provided in multiple ways. The base is provided with a bottle body placement part for placing the detection solution storage bottle. The multiple detection solution storage bottles are placed on the bottle body placement part. The multiple detection solution storage bottles are used to store detection solutions. The detection solutions include buffer washing solution, blocking solution, enzyme conjugate, distilled water and colorimetric solution. Multiple delivery pumps are provided, each corresponding to one of the multiple storage bottles for the test liquid, and the multiple delivery pumps are installed at intervals on the base; Multiple liquid output pipes are provided, each corresponding to one of the multiple delivery pumps. The input end of each liquid output pipe is connected to the delivery pump, and the output end of each liquid output pipe is connected to the horizontal movable liquid filling seat. Multiple test liquid input pipes are provided, each corresponding to one of the multiple delivery pumps. The input end of each test liquid input pipe is connected to the test liquid storage bottle, and the output end of each test liquid input pipe is connected to the delivery pump.

4. The protein blotting device according to claim 1, characterized in that, Also includes: A membrane strip holder is provided above the incubation tray placement part. The membrane strip holder is provided with multiple placement structures corresponding to the multiple incubation slots of the incubation tray placed on the incubation tray placement part. Each placement structure is provided with a placement part for detachably placing the membrane strip. A lifting drive device is installed on the base. The film strip holder is installed on the lifting drive device and can move vertically under the drive of the lifting drive device. The holder and the film strip placed on the holder can extend into the incubation tank under the drive of the lifting drive device.

5. The protein blotting apparatus according to any one of claims 1 to 4, characterized in that, Also includes: A hanging rack is provided above the incubation tray placement area; The air outlet structure includes: Multiple air outlets are provided, and the multiple air outlets are installed at intervals on the suspension frame, with the air outlets of the air outlets facing the incubation tank.

6. The protein blotting apparatus according to any one of claims 1 to 4, characterized in that, Also includes: A transparent light shield is used to cover the outer periphery of the base; A second lifting drive device is disposed on the outside of the base. The transparent light shield is mounted on the second lifting drive device and can move up and down in the vertical direction under the drive of the second lifting drive device.

7. The protein blotting apparatus according to claim 6, characterized in that, The air outlet structure is installed inside the transparent light shield, and the air outlet structure can move vertically relative to the incubation tray placement part under the drive of the second lifting drive device.

Images