The coding sequence of the flowering regulatory gene AP1 in Cymbidium sinense and its application

By isolating and overexpressing the Guolan flowering regulatory gene AP1, recombinant expression vectors are constructed and transformed into plants, the problems of long breeding cycles and unpredictable traits of Guolan were solved, and the regulation of the development of Guolan and ornamental traits were improved were achieved.

CN115927375BActive Publication Date: 2025-08-12ENVIRONMENTAL HORTICULTURE RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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Patent Information

Application Number
CN202211115633.8
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2022-09-14
Publication Date
2025-08-12
Estimated Expiration
2042-09-14

AI Technical Summary

Technical Problem

The prior art has problems of long cycles and difficult to predict traits in the breeding of national orchid varieties, and it is difficult to efficiently improve ornamental traits through modern molecular biotechnology.

Method used

By isolating and overexpressing the national orchid flowering regulatory gene AP1 from the Chinese orchid orchid variety ‘white ink’, a recombinant expression vector was constructed and transformed into plants, and the transformation of petals and petals into sepals was improved.

Benefits of technology

The regulation of the organ development of the orchid inflorescences has been achieved, the lateral branch inflorescence has increased, the petals have abnormal development, and the petals in the flowers have transformed into sepals, which has improved the improvement effect of the ornamental traits of the ornamental traits.

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Abstract

The present invention discloses a coding sequence for the AP1 flowering regulatory gene in Chinese orchids and its application. This orchid floral organ development regulatory gene was isolated from the floral organ cDNA of the Chinese orchid cultivar 'Bai Mo'. Increasing its expression can cause loss of apical dominance in Arabidopsis thaliana, an increase in lateral inflorescences, irregular arrangement of florets on the inflorescence axis, and abnormal petal development in flowers, with the occurrence of fewer petals or petals transforming into sepals. In Chinese orchids, a significant positive correlation was found between gene expression and an increase in sepals, and expression was significantly elevated in variants with a tree-forked flower pattern, indicating that this gene promotes sepal development in Chinese orchids. The Chinese orchid flowering regulatory gene disclosed herein can be used to study the molecular mechanisms of floral organ development in orchidaceae plants and improve flowering traits in plants.
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