A vpr mutant protein capable of activating gene expression in saccharomyces cerevisiae
By randomly mutating and screening the VPR protein in Saccharomyces cerevisiae, a VPR mutant protein with high transcriptional activation efficiency was constructed. Combined with the CRISPRa system, the problem of low gene activation efficiency in Saccharomyces cerevisiae was solved, and gene expression was significantly improved.
Patent Information
- Application Number
- CN202310083723.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2023-02-08
- Publication Date
- 2026-07-24
- Estimated Expiration
- 2043-02-08
AI Technical Summary
The existing VPR gene activation domain in Saccharomyces cerevisiae has low activation efficiency, which limits the application of CRISPR technology in gene regulation and yield enhancement in Saccharomyces cerevisiae.
By randomly mutating the VPR protein in Saccharomyces cerevisiae, fluorescent proteins and resistance genes were designed as screening markers, a mutant library was constructed, and VPR mutant proteins with high transcriptional activation efficiency were obtained through high-throughput screening. Gene activation was then achieved by combining the CRISPRa system.
It significantly improved the activation efficiency of Saccharomyces cerevisiae genes. The mutant protein could increase the activation intensity of the target gene by 14.7 times, which is much higher than the activation intensity of the original VPR, thus achieving efficient gene expression regulation.