Fluorescence Lifetime Detection System and Detection Method

By using quantum dot labeling and detecting its fluorescence lifetime, the problem of insufficient accuracy of the optical detection system is solved, and higher detection accuracy and sensitivity are achieved, especially in organ lesion detection, which significantly improves judgment accuracy.

CN116026800BActive Publication Date: 2025-08-01FUDAN UNIVERSITY
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Patent Information

Application Number
CN202111258438.6
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2021-10-27
Publication Date
2025-08-01
Estimated Expiration
2041-10-27

AI Technical Summary

Technical Problem

The accuracy and sensitivity of existing optical detection systems or detection methods are insufficient, there is a misdiagnosis rate, weak autofluorescence signals and short lifespan, resulting in the detection accuracy needs to be improved.

Method used

The detector is labeled with quantum dots, the excitation light provided by the luminescent unit is used to fluoresce, the fluorescence signal is detected through a photodetector, and the time-dependent single-photon counting unit is used to obtain time-based fluorescence lifetime data, and quantum dots of core-shell structure such as AgInS2/ZnS are combined to improve the fluorescence lifetime and signal intensity.

Benefits of technology

The fluorescence lifetime of quantum dots is longer and the signal is stronger, which reduces detection difficulty, improves detection accuracy and sensitivity, and can judge whether the organ has lesions based on changes in the microenvironment.

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Abstract

A fluorescence lifetime detection system and a detection method. The fluorescence lifetime detection system includes: a labeling unit for providing quantum dots and for labeling a detection object with the quantum dots; a light emitting unit for providing excitation light, the excitation light being projected onto the detection object labeled with the quantum dots, and the quantum dots being capable of generating fluorescence; a photodetector for detecting the fluorescence generated by the quantum dots and collecting fluorescence signals; and a time-correlated single photon counting unit for obtaining time-based fluorescence lifetime data based on the fluorescence signals. The quantum dots can exhibit different fluorescence lifetimes according to the different microenvironments of the detection object itself. Thus, by detecting the fluorescence generated by the quantum dots, the microenvironment of the detection object can be detected according to the fluorescence lifetime of the quantum dots. Moreover, compared with the autofluorescence of the detection object, the fluorescence lifetime of the fluorescence generated by the quantum dots is longer, and the fluorescence signal obtained by detecting the fluorescence generated by the quantum dots is stronger, which can reduce the detection difficulty and improve the detection accuracy.
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