Active aggregated peptides and uses thereof
By using the active aggregation peptides HlyA218, HlyA60, H12, H1, and H2 from Escherichia coli CFT073 to mediate the formation of active inclusion bodies from recombinant proteins, the problems of limited expression, difficult purification, and immobilization in recombinant protein production have been solved, achieving efficient and economical recombinant protein production.
CN116102628BActive Publication Date: 2026-05-29SOUTH CHINA UNIV OF TECH
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- SOUTH CHINA UNIV OF TECH
- Filing Date
- 2022-08-17
- Publication Date
- 2026-05-29
Smart Images
Figure CN116102628B_ABST
Abstract
The application discloses active aggregation peptides and application thereof, and belongs to the technical field of bioengineering. The active aggregation peptides are derived from alpha-hemolysin HlyA of Escherichia coli CFT073, and comprise five sequences, namely HlyA218, HlyA60, H12, H1 and H2. The active aggregation peptides can mediate the active inclusion body formation of a target protein in a host cell by being fused with the target protein for expression, and the short peptides have high protein activity aggregation rate and the potential of stimulating the rapid growth of a recombinant bacterium. The active aggregation peptides can be applied to column-free purification and in-situ immobilization of a recombinant protein, and have low production cost and simple steps.
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