A method for identifying whether a sample to be tested is infected with SGDV and a special primer pair thereof

By developing specific primer pairs and RT-PCR technology, the problem of identifying SGDV infection in Litopenaeus vannamei was solved, enabling rapid and accurate virus detection and reducing shrimp larvae mortality and economic losses.

CN116144648BActive Publication Date: 2026-07-03BEIJING UNIV OF CHEM TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
BEIJING UNIV OF CHEM TECH
Filing Date
2022-05-05
Publication Date
2026-07-03

AI Technical Summary

Technical Problem

Currently, there is a lack of effective methods to identify whether Pacific white shrimp are infected with shrimp glass disease virus (SGDV). The glass disease caused by this virus is highly contagious and pathogenic, leading to large-scale mortality of shrimp larvae and causing significant economic losses to the aquaculture industry.

Method used

A primer pair (the nucleotide sequences of primer F and primer R are shown in SEQ ID NO: 1 and SEQ ID NO: 2) was developed to identify whether a sample to be tested is infected with SGDV by RT-PCR technology. The target sequence is 131 bp in length and the nucleotide sequence of the target sequence is shown in SEQ ID NO: 4.

Benefits of technology

It enables accurate identification of SGDV, allowing for quick determination of whether a sample is infected with the virus, thus helping to prevent and control the spread of SGDV and reduce shrimp larvae mortality.

✦ Generated by Eureka AI based on patent content.

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Abstract

The application discloses a method for identifying whether a sample to be detected is infected with a shrimp glass disease virus (SGDV) and a special primer pair thereof. The inventor of the application finds a new small ribonucleic acid virus SGDV in Penaeus vannamei Boone, and the nucleotide sequence of the virus is shown in SEQ ID NO: 3. Experiments prove that a primer pair composed of a primer F shown in SEQ ID NO: 1 and a primer R shown in SEQ ID NO: 2 can accurately identify whether the sample to be detected is infected with the SGDV. The application has important application value, and helps us to deepen the understanding of virus diversity, provides guidance for the breeding of the Penaeus vannamei Boone and has important significance for preventing infectious diseases in aquaculture.
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