Preparation method of bacterial cellulose-based multi-layer structure functional bionic scaffold

By combining a bacterial cellulose-based multilayer structure with a microchannel system, the problems of multilayer structure and uneven coating in biomimetic scaffolds were solved, achieving high tensile strength of the scaffold and uniform application of functional coatings, thus improving the performance and safety of tissue engineering scaffolds.

CN116214795BActive Publication Date: 2026-05-12JIAXING UNIV
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Patent Information

Application Number
CN202211549934.1
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2022-12-05
Publication Date
2026-05-12
Estimated Expiration
2042-12-05

AI Technical Summary

Technical Problem

Existing technologies struggle to effectively mimic the multi-layered structure and dense layers of tissues when fabricating complex biomimetic scaffolds, and the coating is unevenly applied to the scaffold, affecting cell growth and functionality.

Method used

Using bacterial cellulose as the dense layer material, combined with a microchannel system and patterning technology, an interlaced nanoscale network structure is generated on a porous scaffold by culturing bacterial solution, and the coating is uniformly coated by using a microchannel system to prepare a bacterial cellulose-based multilayer functional biomimetic scaffold.

Benefits of technology

The biomimetic scaffold achieved a multi-layered structure and uniform coating of functional components, which improved the tensile strength and functionality of the scaffold, shortened the bacterial fermentation cycle, and reduced the risk of in vivo transplantation.

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Abstract

The application relates to a preparation method of a bacterial cellulose-based multilayer structure functional bionic scaffold, which comprises the following steps: firstly, injecting a biomaterial solution into a silica gel model with a microchannel system to perform freeze drying to obtain a porous biological scaffold; secondly, performing crosslinking treatment on the porous biological scaffold; thirdly, inoculating a culture bacteria solution with a Xylobacter concentration of 10 9 ~ 10 15 individuals / mL on the crosslinking-treated porous biological scaffold, and performing static culture and freeze drying to obtain a bacterial cellulose-based composite scaffold; and finally, adopting a coating solution to coat the bacterial cellulose-based composite scaffold through a microchannel system, and performing demolding treatment to obtain the bacterial cellulose-based multilayer structure functional bionic scaffold. The application provides a functional coating process for the multilayer bionic scaffold by using the shrinkage space of the treated material, the micro-pore channel and the injection method of the patterned drainage network, effectively prevents the penetration of the coating solution, and realizes the perfect solidification of the coating solution on the scaffold.
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