An in-situ sealed and sterilized HEPA filter housing and method of use

Abstract

This invention discloses an in-situ sealed and sterilized HEPA filter housing, comprising a biosafety shut-off valve, an upper dust-generating duct, a HEPA filter, a housing, an air vent cover, a ventilation cover, a sterilization connector, and a pressure testing connector. This invention also discloses a method for using the in-situ sealed and sterilized HEPA filter housing. This invention can seal the housing, allowing for in-situ sterilization of the HEPA filter and housing airtightness testing via the sterilization and pressure testing connectors. Simultaneously, during the testing of the enclosure structure within a high-level biosafety laboratory, it serves to isolate the biosafety laboratory and prevent contamination of facilities, equipment, and the environment.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of biosafety, air-tightness device, in particular to a HEPA filter box sealed and disinfected in situ, and also relates to a use method of the HEPA filter box sealed and disinfected in situ, which is applied to life science, physical and chemical and high-level biosafety laboratory, and medical operating room and biopharmaceutical workshop and other occasions requiring air-tightness test of air supply and exhaust ducts and room enclosure. BACKGROUND

[0002] The HEPA filter box is a metal box commonly installed on the wall or roof of a house to purify indoor and outdoor air through a high-efficiency filter. The HEPA filter box is composed of a stainless steel box, a biosafety sealing valve, a high-efficiency filter and a air port baffle.

[0003] The HEPA filter box device (filter device) is one of the key protective facilities and equipment related to laboratory biosafety, and is widely used in high-level biosafety laboratories. The HEPA filter box is usually installed on site and then needs to be tested for box air tightness and high-efficiency filter leak detection. Box air tightness testing is generally divided into factory testing and on-site testing. Factory testing cannot replace on-site testing, and on-site testing is still required. On-site testing does not have the detection conditions to detect in the factory way, so the filter box needs to be tested together with the room or duct. Detection process: usually first close the box biosafety sealing valve, test the filter device and the room together; or close the valve on the duct, test the duct, filter device and room together. High-efficiency filter leak detection refers to determining whether the high-efficiency filter itself and its installation have obvious leakage. Dust source generator is used to generate dust upstream of the filter, and aerosol photometer is used to detect aerosol concentration upstream and downstream of the filter to determine whether the filter has leakage. Laboratory filter box disinfection process: in the disinfection mode, the air supply and exhaust ducts and the room form a closed loop space, and the disinfection machine generates disinfection steam or dry mist to form directional airflow under the action of external force to disinfect the filter and the surface of the room. The disinfection machine can be located in the room and the air flows directionally under the action of the circulating fan, or a hose is connected to the special disinfection port of the ventilation duct to make the air form directional flow and circulate disinfection by using the fan of the disinfection machine.

[0004] The above methods can indirectly check the overall airtightness and integrity of HEPA filter housings. However, existing technologies have the following drawbacks: 1. Airtightness testing of filter housings using ordinary hoods with blocked air vents is bulky and heavy, making installation impossible in the confined space above laboratory equipment, and hindering independent airtightness testing; 2. The bio-sealing valves of general filter housings and pipelines are typically located on the equipment layer (outside the laboratory work area). Leaks pose a significant biosafety risk, and incomplete sealing is difficult to detect; 3. Liquid-sealed gel adhesives, under circulating ventilation, inevitably blow in external dust and foreign objects, which can become stuck at the valve openings, leading to poor sealing; 4. Poor sealing at the flexible connections between pipelines and filter housings fails to meet the leakage rate requirements for room and pipeline airtightness testing. Numerous valves and connections on pipelines make leak detection difficult, and testing pipelines and filter housings together is unsuitable; 5. During room airtightness testing, incomplete disinfection of filters and pipelines may result in the presence of highly pathogenic pathogens, posing a biohazard to laboratory personnel. 6. During the disinfection of the circulating airflow from the fan, the front side of the filter is thoroughly disinfected, but the back side may not be completely disinfected. 7. Existing technologies generally suffer from poor pressure resistance and airtightness. 8. In-situ disinfection and airtightness testing are performed separately, which is cumbersome and poses biosafety risks during testing.

[0005] This device utilizes a dual-seal structure of mechanical and liquid seals at the biosafety airtight valve port, ensuring mechanical locking to prevent loosening, high pressure resistance, and reliable sealing. The biosafety airtight valve and vent cover allow for independent testing of the HEPA filter housing's airtightness, as well as in-situ, independent disinfection of the HEPA filter. Double-sided disinfection of the filter (top and bottom) ensures thorough disinfection and improves biosafety during filter replacement. The vent cover, made of relatively low-density aluminum with a single-layer structure, uses silicone sealant for easy disassembly and installation. The silicone sealant is removable, maintaining a clean appearance and allowing for reuse. The folded edge design ensures high-strength, non-deformable material and reliable sealing. During room airtightness testing, the HEPA filter housing at the supply and exhaust vents is a primary biosafety risk point. Given the numerous supply and exhaust vents in the laboratory, the vent cover effectively seals the HEPA filter housing vents, closing all external access points. This facilitates leak detection within the laboratory and prevents laboratory personnel from contacting potential sources of risk. The opening and closing adjustment device of the biosafety airtight valve core is located inside the high-efficiency filter housing and has a sealing mechanism. Leak detection of the filter can be performed on the sealing structure at the same time, making it easy to detect the leakage of biohazardous factors into the external environment caused by the aging of the seal. Summary of the Invention

[0006] The purpose of this invention is to address the current state of the technology by providing an air vent cover for in-situ disinfection and airtightness testing of a ceiling-mounted HEPA filter housing, and also to provide a method for using the air vent cover for in-situ disinfection and airtightness testing of a HEPA filter housing.

[0007] To achieve the above-mentioned objectives, the present invention provides the following technical solution:

[0008] A HEPA filter housing for in-situ sealed disinfection includes a housing, the top of which is connected to the bottom of an air duct, and the top of the air duct is connected to a supply and exhaust air duct. The diameter of the top of the housing is larger than the diameter of the bottom of the air duct. A biosafety shut-off valve core is installed in the inner cavity at the connection between the housing and the air duct. An annular mounting frame is installed on the inner wall of the housing, located below the biosafety shut-off valve core. A HEPA filter is installed at the center of the mounting frame, and the outer edge of the HEPA filter is fixed to the inner ring of the mounting frame. The gap between the inner ring of the mounting frame and the annular outer edge of the HEPA filter is sealed with sealant. A vent valve is installed on the mounting frame, the top of which is connected to one end of an upper dust-generating duct, and the other end of the upper dust-generating duct extends above the HEPA filter. A ball valve is installed at the bottom of the vent valve. An air vent cover or ventilation cover is installed at the bottom of the housing.

[0009] As described above, the biosafety airtight valve core includes an upwardly converging inclined plate and a base plate connected to the bottom of the inclined plate. An inclined plate groove is provided on the outer edge of the bottom of the inclined plate, and the inclined plate groove is filled with jelly glue. Two sealing grooves are provided on the bottom of the outer wall of the air duct, and a layer of air duct sealing ring is provided in each sealing groove. When the biosafety airtight valve core is closed, the jelly glue is inserted into the bottom of the side wall of the air duct, and the air duct sealing ring abuts against the inner wall of the inclined plate groove.

[0010] As described above, the bottom surface of the base plate is connected to the top surface of the ball nut. The lifting mechanism screw is located on the central axis of the housing and passes through the base plate of the biosafety airtight valve core and the ball nut from top to bottom. The ball nut and the lifting mechanism screw form a ball screw structure. The intermediate transmission rod is perpendicular to the lifting mechanism screw. The first active bevel gear sleeved at one end of the intermediate transmission rod meshes with the first driven bevel gear sleeved at the bottom end of the lifting mechanism screw. The intermediate transmission rod is perpendicular to the adjusting screw and the second driven bevel gear sleeved at the other end of the intermediate transmission rod meshes with the second active bevel gear sleeved at the top end of the adjusting screw. A polygonal groove is provided at the bottom end of the adjusting screw.

[0011] As described above, the ball nut is a two-layer inverted stepped shape, with the upper layer being larger than the lower layer. The upper layer is the base of the ball nut, and a third pin hole in the horizontal direction is provided on the inner wall of the base of the ball nut. The spring pin is set in the third pin hole. When the valve core of the biosafety airtight valve closes the air duct, the spring pin is inserted into the first pin hole on the screw of the lifting mechanism; when the valve core of the biosafety airtight valve opens the air duct, the ball nut moves downward, and at the same time the spring pin retracts into the ball nut.

[0012] A support plate is provided under the base plate as described above. A support plate connecting plate is provided at each of the four corners of the support plate. The upper end face of each support plate connecting plate is connected to the inner surface of the top wall of the box. A ventilation hole is provided in the central area of ​​the support plate. The horizontal plate and the vertical plate of the first gear mounting plate are vertically connected, and the horizontal plate of the first gear mounting plate is fixed to the lower surface of the support plate. The horizontal plate and the vertical plate of the second gear mounting plate are vertically connected, and the horizontal plate of the second gear mounting plate is vertically fixed to the inner surface of the side wall of the box. The vertical plate of the second gear mounting plate is vertically fixed to the inner surface of the top wall of the box.

[0013] Below the ball nut, the lifting mechanism screw also passes through a horizontal plate consisting of a first adjusting ring, a support plate, and a first gear mounting plate. The first adjusting ring is connected and fixed to the lifting mechanism screw via a first pin. The lower part of the lifting mechanism screw has a shoulder, the upper surface of which abuts against the lower surface of the horizontal plate of the first gear mounting plate. The first driven bevel gear is located below the horizontal plate of the first gear mounting plate.

[0014] The two ends of the intermediate transmission rod are rotatably connected to the vertical plate of the first gear mounting plate and the vertical plate of the second gear mounting plate, respectively. The second driven bevel gear is above the horizontal plate of the second gear mounting plate. The second adjusting ring on the intermediate transmission rod, located between the first driving bevel gear and the vertical plate of the first gear mounting plate, is fixed to the intermediate transmission rod by the second pin.

[0015] The adjusting screw is perpendicular to the horizontal plate of the second gear mounting plate and is rotatably connected to the horizontal plate of the second gear mounting plate. The second driving bevel gear is located on the horizontal plate of the second gear mounting plate. An adjusting screw adjustment ring is provided between the second gear mounting plate and the second driving bevel gear, and also below the second gear mounting plate. The two adjusting screw adjustment rings are respectively connected to the adjusting screw through adjusting screw pins. An adjusting hole is provided on the mounting frame, and the lower end of the adjusting screw is rotatably connected to the adjusting hole.

[0016] As described above, the bottom surface of the ball nut base is connected to the upper surface of the guide arm. The lower layer of the ball nut extends to the bottom of the guide arm through the opening in the middle of the guide arm. The guide columns, which are symmetrically distributed with respect to the central axis of the housing, are perpendicular to the guide arm and are slidably connected with damping. The upper and lower ends of the guide columns are connected to the inner wall of the top surface of the air duct and the support plate, respectively. The screw of the lifting mechanism passes through the guide arm.

[0017] As described above, when the ventilation cover is installed at the bottom of the box, one side of the ventilation cover is detachably hinged to the third pin on the bottom side wall of the box via a hook. The other side of the ventilation cover is provided with suction plates, which are magnetically attracted to the magnetic bolts provided on the inner side wall of the bottom of the box.

[0018] As described above, each inner side wall of the housing is provided with a fixing plate with screw holes at its bottom. When the bottom of the housing is connected to the air vent cover, the threaded end of the fixing bolt passes through the air vent cover and connects to the screw hole of the fixing plate with screw holes. A rubber cap is provided between the bolt head of the fixing bolt and the air vent cover. The gap between the air vent cover and the housing is filled with sealing silicone. A pair of handles are provided on the bottom surface of the air vent cover. The disinfection connector is sealed through the air vent cover. The top of the disinfection connector is a quick-connect threaded connector, and the bottom of the disinfection connector is located on the outside of the bottom surface of the air vent cover. The pressure testing connector includes a male connector that is sealed through the air vent cover and a female connector that is detachably connected to the bottom of the male connector. A high-efficiency filter is provided on the top of the male connector, and the bottom of the male connector is located on the outside of the bottom surface of the air vent cover.

[0019] A method for using an in-situ sealed and sterilized HEPA filter housing, utilizing the in-situ sealed and sterilized HEPA filter housing as described above, includes a housing airtightness test step:

[0020] Step 1: Close the biosafety shut-off valve core: Turn the adjusting screw to close the biosafety shut-off valve core;

[0021] Step 2: Install the air vent cover;

[0022] Step 3: Insert the female end of the pressure test connector into the male end of the pressure test connector, connect the air source from the lower end of the female end of the pressure test connector, connect the bottom end of the sterilization connector to the pressure gauge, and after filling with the set amount of pressure test gas, turn off the air source and observe the airtightness of the chamber.

[0023] Step 4: After the test is completed, disconnect the gas source. The pressure test gas will be discharged downward from the pressure test connector into the housing. Remove the female connector of the pressure test connector.

[0024] The above-described method for using an in-situ sealed sterilization HEPA filter housing includes an in-situ sterilization step for the HEPA filter:

[0025] Step 1: Close the biosafety shut-off valve core: Turn the adjusting screw to close the biosafety shut-off valve core;

[0026] Step 2: Set up the disinfection gas circulation path and place several disinfection indicator tablets at different locations inside the box;

[0027] For the first disinfection gas circulation path, open the vent valve and connect the bottom of the vent valve to the high-efficiency filter through the air tube;

[0028] For the second type of disinfection gas circulation path, open the vent valve, connect the top of the vent valve to one end of the upper dust generation pipeline, the other end of the upper dust generation pipeline is a movable end and extends to the top of the HEPA filter, the quick-connect threaded connector at the top of the disinfection connector is connected to one end of the lower dust generation pipeline, and the other end of the lower dust generation pipeline is connected to the bottom of the vent valve.

[0029] Step 3: Install the air vent cover;

[0030] Step 4: Connect the air inlet of the circulating sterilizer to the bottom of the sterilization connector, insert the upper end of the female connector of the pressure test connector into the male connector of the pressure test connector, connect the bottom end of the female connector of the pressure test connector to the return port of the circulating sterilizer, and then turn on the circulating sterilizer.

[0031] Step 5: After the circulation disinfection, disconnect the circulation disinfection machine from the disinfection connector and the pressure test connector in sequence, remove the female part of the pressure test connector, and remove the air vent cover.

[0032] Compared with the prior art, the present invention has the following advantages:

[0033] 1. The air vent cover of this invention adopts a single-layer structure of lightweight and high-strength aluminum or polyurethane board. The frame support strip and folded edge design are small in size, making it easy to install and use in small spaces in the laboratory. It can be lifted and installed by a single person, reducing the skill requirements for personnel operation and reducing the risk of personnel falling.

[0034] 2. The valve adjustment of the biosafety shut-off valve is located inside the HEPA filter housing. The opening and closing of the biosafety shut-off valve in the filter housing is operated in the laboratory. The seal is located in the adjustment hole on the filter mounting frame. The adjustment hole has a double seal to avoid the risk of leakage to the external environment due to the aging of the valve adjustment structure seal, and also facilitates aerosol particle leak detection.

[0035] 3. The biosafety closed valve of this invention adopts a dual-seal structure of mechanical seal and liquid seal. The liquid jelly seal has good fluidity, and the radial compression sealing ring of the valve port has high pressure resistance, which effectively avoids impurities in the jelly and poor fluidity. The dual seal has better sealing performance, and the valve port core has a locking mechanism when closed. The biosafety closed valve has high pressure resistance.

[0036] 4. Use vent covers and biosafety airtight valves to isolate the pipes from the filter housing, thereby creating a separate space and reducing the risk of leakage;

[0037] 5. When an airtightness test is required on the HEPA filter housing, a separate space can be formed using the vent cover and biosafety airtight valve, which can be completed on-site without disassembling the filter housing. This avoids the possibility of biohazards to laboratory personnel caused by residual highly pathogenic pathogens in the HEPA filter and pipeline.

[0038] 6. The housing and HEPA filter can be disinfected in situ and separately. Double-sided disinfection can be achieved by switching disinfection pipelines, resulting in more thorough disinfection.

[0039] 7. For common problems of poor pressure resistance and air tightness, the present invention is equipped with a fixing bolt seal to tighten the air vent cover to the box body. At the same time, the frame is equipped with a sealing strip and sealing gasket, so that the pressure resistance and air tightness reliability are doubly guaranteed.

[0040] 8. During room airtightness testing, the HEPA filter is enclosed in the enclosure using an air vent cover. This isolates hazardous sources, which is beneficial for the protection of laboratory personnel and improves the biosafety of testing activities. It is also easy to disassemble and install, the sealing silicone can be removed, ensuring a clean and aesthetically pleasing interior with no sharp corners or protruding structures, eliminating blind spots and meeting general laboratory biosafety requirements. Furthermore, the valve core lifting structure of this biosafety closed valve uses a screw structure. Manual adjustment is achieved through a two-stage bevel gear transmission, which drives the screw to rotate, realizing different valve core opening degrees. The opening degree is maintained using a guide. The arm and guide column exhibit static friction, and the screw and valve core are locked in the fully closed state via a pin and pin hole. The valve core has low mechanical transmission force, stable transmission relationship, and avoids wear and jamming of the transmission structure. In addition, the bevel gear installation of this invention achieves right-angle meshing of the bevel gears by installing a bevel gear on each side of the right-angle mounting plate. The axial positioning of the bevel gears is adjusted by adjusting the adjusting ring, pin, and shaft shoulder. The bevel gears are connected and fixed to the housing by the mounting plate position. The transmission and installation fixing structure is simple and compact, with a large adjustment space, which can achieve smooth transmission process and convenient operation. Attached Figure Description

[0041] Figure 1 This is a schematic diagram (half-sectional view) of the installation of the present invention;

[0042] Figure 2 This is a top view of the internal mounting frame of the present invention;

[0043] Figure 3 A schematic diagram of the external structure of the air vent cover of the present invention;

[0044] Figure 4 This is a schematic diagram of the internal structure of the air vent cover of the present invention;

[0045] Figure 5 This is a schematic diagram of the internal structure of the ventilation cover plate of the present invention;

[0046] Figure 6 This is a schematic diagram of the external structure of the ventilation cover plate of the present invention;

[0047] Figure 7 This is an external view of the present invention;

[0048] Figure 8 This is an external view of the structure when the ventilation cover is open.

[0049] Figure 9 A partial cross-sectional view of the valve core locking structure of a biosafety closed valve;

[0050] Figure 10 A partial sectional view of the adjusting screw sealing structure;

[0051] Figure 11 A partial cross-sectional view of the valve core sealing structure of a biological closed valve;

[0052] In the diagram: 1-Air duct; 2-Biosafety airtight valve core; 201-Sloping plate; 202-Base plate; 3-Jelly adhesive; 4-Box body; 5-Third pin; 6-Air outlet cover; 7-Fixing bolt; 8-Rubber cap; 9-Fixing plate with screw holes; 10-Handle; 11-Sealing silicone; 12-Magnetic bolt; 13-Upper dust-generating duct; 14-HEPA filter; 15-Adjusting screw; 1501-Second driving bevel gear; 16-Intermediate transmission rod; 1601-First driving bevel gear; 1602-Second driven bevel gear; 1603-Second adjusting ring; 17-Lifting mechanism screw; 1701- First driven bevel gear; 1702-shoulder; 18-pressure test connector; 19-disinfection connector; 20-high efficiency filter; 21-quick-connect threaded connector; 22-hook; 23-ventilation cover; 24-ventilation hole; 25-suction plate; 26-mounting frame; 27-ventilation valve; 28-guide post; 29-guide arm; 30-support plate connecting plate; 31-ball bearing nut; 32-support plate; 33-spring pin; 34-adjusting screw sealing ring; 35-adjusting hole; 36-air duct sealing ring; 37-first gear mounting plate; 38-first adjusting ring; 39-first pin; 40-second gear mounting plate. Detailed Implementation

[0053] To facilitate understanding and implementation of the present invention by those skilled in the art, the present invention will be further described in detail below with reference to examples. The implementation examples described herein are only for illustration and explanation and are not intended to limit the present invention.

[0054] Example 1:

[0055] A HEPA filter housing for in-situ sealed sterilization includes a housing 4, a biosafety airtight valve core 2, an upper dust-generating duct 13, a HEPA filter 14, a ventilation cover 23, and an air outlet cover 6, as shown. Figure 1As shown, in this embodiment, the housing 4 is a two-layer square stepped structure with openings at the top and bottom. The top of the housing 4 is connected to the bottom of the air duct 1, and the top of the air duct 1 is connected to the supply and exhaust air ducts. The diameter of the top of the housing 4 is larger than the diameter of the bottom of the air duct 1. A biosafety airtight valve core 2 is installed in the inner cavity at the connection between the housing 4 and the air duct 1. The biosafety airtight valve core 2 is used to block or open the air duct 1. A ring-shaped mounting frame 26 is provided on the inner wall of the housing 4 below the biosafety airtight valve core 2. Figure 2 As shown, a HEPA filter 14 is installed at the center of the mounting frame 26. The outer edge of the HEPA filter 14 is fixed to the inner ring of the mounting frame 26. The gap between the inner ring of the mounting frame 26 and the outer ring of the HEPA filter 14 is sealed with sealant. A vent valve 27 is provided on the mounting frame 26. The top of the vent valve 27 is connected to one end of the upper dust-generating pipe 13. The other end of the upper dust-generating pipe 13 is a movable end for exhausting gas and extending above the HEPA filter 14. A ball valve is provided at the bottom of the vent valve 27. The ball valve can be closed or opened to disconnect or connect other vent pipes depending on the application scenario. An air vent cover 6 or a ventilation cover 23 is installed at the bottom of the housing 4 according to different application scenarios. The ventilation cover 23 is used for daily use, and the air vent cover 6 is installed when disinfecting the HEPA filter 14 or conducting a tightness test.

[0056] The sealing method and related structure of the biosafety airtight valve for duct 1: (e.g.) Figure 11 As shown, the biosafety airtight valve core 2 includes an upwardly converging conical inclined plate 201 and a base plate 202 connected to the bottom of the inclined plate 201. The inclined plate 201 plays a horizontal control role when the biosafety airtight valve core 2 moves up and down, preventing the biosafety airtight valve core 2 from tilting in the horizontal direction. The bottom of the air duct 1 is circular, and the shape and size of the bottom of the inclined plate 201 of the biosafety airtight valve core 2 match the circular bottom of the air duct 1. An inclined plate groove is provided on the outer edge of the bottom of the inclined plate 201, and the groove is filled with gel 3. When the biosafety airtight valve core 2 is closed, the gel 3 is inserted into the bottom of the side wall of the air duct 1 to seal the air duct 1. Two sealing grooves are provided on the bottom of the outer wall of the air duct 1, and each sealing groove is provided with a layer of air duct sealing ring 36. When the biosafety airtight valve core 2 is closed, the air duct sealing ring 36 abuts against the inner wall of the inclined plate groove. The combined action of the duct sealing ring 36, the jelly adhesive 3, and the inclined plate 201 can achieve a better sealing effect on the duct 1. The upper part of the duct 1 is connected to the supply and exhaust air duct. The shape of the upper part of the duct 1 and the supply and exhaust air duct can be circular or square. The upper part of the duct 1 is connected to the supply and exhaust air duct through a flange.

[0057] The transmission method of the biosafety shut-off valve: The bottom surface of the base plate 202 is connected to the top surface of the ball nut 31. The lifting mechanism screw 17 is located on the central axis of the housing 4. The lifting mechanism screw 17 passes through the base plate 202 and the ball nut 31 of the biosafety shut-off valve core 2 from top to bottom. The ball nut 31 and the lifting mechanism screw 17 form a ball screw structure. The intermediate transmission rod 16 is perpendicular to the lifting mechanism screw 17. The first active bevel gear 1601 sleeved on one end of the intermediate transmission rod 16 is connected to the lifting mechanism. The first driven bevel gear 1701 sleeved on the bottom end of the screw 17 is engaged. The first driving bevel gear 1601 on the intermediate transmission rod 16 and the first driven bevel gear 1701 on the lifting mechanism screw 17 are the first pair of meshing bevel gears. The intermediate transmission rod 16 is perpendicular to the adjusting screw 15 and is connected by the second pair of meshing bevel gears. The second pair of meshing bevel gears includes the second driven bevel gear 1602 sleeved on the other end of the intermediate transmission rod 16 and the second driving bevel gear 1501 sleeved on the top end of the adjusting screw 15. The bottom end of the adjusting screw 15 is provided with a polygonal groove to allow rotation of the adjusting screw 15 using an external adjusting rod. One end of the external adjusting rod is a polygonal head that fits into the polygonal groove. The polygonal head of the external adjusting rod is inserted into the polygonal groove of the adjusting screw 15, causing the adjusting screw 15 to rotate. This rotation then sequentially drives the ball nut 31, guide arm 29, and biosafety shut-off valve core 2 to move up and down via the second pair of meshing bevel gears, the intermediate transmission rod 16, the first pair of meshing bevel gears, and the lifting mechanism screw 17. The aforementioned first driving bevel gear 1601, first driven bevel gear 1701, second driven bevel gear 1602, and second driving bevel gear 1501 are all fixed to the lifting mechanism screw 17, the intermediate transmission rod 16, and the adjusting screw 15 respectively by welding or radial bolts.

[0058] Locking method of biosafety shut-off valve: such as Figure 9As shown, the lifting mechanism screw 17 has a first pin hole, and the ball nut 31 has a spring pin 33. The ball nut 31 is a two-layer cylindrical stepped structure with a larger upper layer and a smaller lower layer. The upper layer is the base of the ball nut 31, and the top of the ball nut 31 is connected to the bottom surface of the base plate 202. A third pin hole in the horizontal direction is provided on the inner wall of the base of the ball nut 31, and the spring pin 33 is set in the third pin hole. When the biosafety airtight valve core 2 blocks the air duct 1, the spring pin 33 is inserted into the first pin hole of the lifting mechanism screw 17 under the action of the spring thrust. The relative position of the ball nut 31 and the lifting mechanism screw 17 is locked, and the rising position of the biosafety airtight valve core 2 is fixed and kept in the closed state with the air duct 1. When the biosafety airtight valve core 2 needs to open the air duct 1, the adjusting screw 15 is manually turned by the external adjusting rod. The adjusting screw 15 drives the lifting mechanism screw 17 to rotate through the first pair of meshing bevel gears and the second pair of meshing bevel gears. The rotation of the lifting mechanism screw 17 causes the ball nut 31 to move downward. At the same time, the spring pin 33 retracts into the ball nut 31, and the ball nut 31 drives the biosafety airtight valve core 2 to open the air duct 1.

[0059] The connection and installation method of the transmission structure of the biosafety closed valve: The transmission structure of the biosafety closed valve includes the aforementioned ball nut 31, lifting mechanism screw 17, intermediate transmission rod 16, and adjusting screw 15. To fix the position of the rotating shaft of the lifting mechanism screw 17, the present invention provides a support plate 32, a first adjusting ring 38, and a first pin 39; to fix the positions of the rotating shafts of the intermediate transmission rod 16 and the adjusting screw 15, the present invention provides a first gear mounting plate 37, a second gear mounting plate 40, and adjusting holes 35 opened on the mounting frame 26. In this embodiment, the support plate 32 is located below the base plate 202, and a support plate connecting plate 30 is respectively provided at the four corners of the support plate 32. The upper end surface of each support plate connecting plate 30 is connected to the inner surface of the top wall of the housing 4. A vent hole is provided in the central area of ​​the support plate 32, allowing airflow (supply air) at the upper end of the support plate 32 or airflow (exhaust air) at the lower end of the support plate 32 to pass through, reducing the resistance of airflow in the housing and thus reducing unnecessary energy consumption. The first gear mounting plate 37 includes a horizontal plate and a vertical plate. The horizontal plate and the vertical plate of the first gear mounting plate 37 are vertically connected and form an "L" or "T" shape. The horizontal plate of the first gear mounting plate 37 is fixed to the lower surface of the support plate 32. In this embodiment, the horizontal plate of the first gear mounting plate 37 is fixed to the support plate 32 by bolts passing through part of the ventilation holes. The second gear mounting plate 40 also includes a horizontal plate and a vertical plate. The horizontal plate and the vertical plate of the second gear mounting plate 40 are vertically connected. The horizontal plate of the second gear mounting plate 40 is vertically fixed to the inner surface of the side wall of the housing 4, and the vertical plate of the second gear mounting plate 40 is connected to the inner surface of the top wall of the housing 4. The lower surface of the horizontal plate of the second gear mounting plate 40 is connected to the inner surface of the side wall of the housing 4 by right-angled triangular prisms or inclined plates, thereby fixing the angle between the horizontal plate of the second gear mounting plate 40 and the side wall of the housing 4, and further fixing the position of the second gear mounting plate 40. The specific fixing scheme for the lifting mechanism screw 17 shaft, intermediate transmission rod 16 shaft, and adjusting screw 15 shaft is as follows:

[0060] (1) Fixing the shaft of the lifting mechanism screw 17: Below the ball nut 31, the lifting mechanism screw 17 also passes through a horizontal plate consisting of a first adjusting ring 38, a support plate 32, and a first gear mounting plate 37. The first adjusting ring 38 is connected and fixed to the lifting mechanism screw 17 via a first pin 39. In this embodiment, the first adjusting ring 38 and the lifting mechanism screw 17 are respectively provided with a fourth pin hole and a second pin hole, and the first pin 39 passes horizontally through the fourth pin hole and the second pin hole. The lower part of the lifting mechanism screw 17 is provided with a shoulder 1702, and the upper surface of the shoulder 1702 abuts against the lower surface of the horizontal plate of the first gear mounting plate 37. The position of the shaft of the lifting mechanism screw 17 is fixed by the upper and lower locking of the first adjusting ring 38 and the shoulder 1702. The first driven bevel gear 1701 is located below the horizontal plate of the first gear mounting plate 37.

[0061] (2) Fixing the shaft of the intermediate transmission rod 16: The two ends of the intermediate transmission rod 16 are rotatably connected to the vertical plate of the first gear mounting plate 37 and the vertical plate of the second gear mounting plate 40, respectively. In this embodiment, the vertical plates of the first gear mounting plate 37 and the second gear mounting plate 40 are provided with shaft holes, and there are bushings in the shaft holes. The bushings play a supporting role to prevent the shaft of the intermediate transmission rod 16 from deforming. The two ends of the intermediate transmission rod 16 pass through the shaft holes of the first gear mounting plate 37 and the second gear mounting plate 40, respectively, and are respectively fitted and fixed with the second driven bevel gear 1602 and the first driving bevel gear 1601. The second driven bevel gear 1602 is above the horizontal plate of the second gear mounting plate 40. The intermediate transmission rod 16 is provided with a second adjusting ring 1603 between the first driving bevel gear 1601 and the vertical plate of the first gear mounting plate 37. The second adjusting ring 1603 is fixed to the intermediate transmission rod 16 by a second pin.

[0062] (3) Fixing the adjusting screw 15 shaft: The adjusting screw 15 is perpendicular to the horizontal plate of the second gear mounting plate 40 and rotatably connected to the horizontal plate of the second gear mounting plate 40. A second driving bevel gear 1501 is sleeved on the upper end of the adjusting screw 15, and the second driving bevel gear 1501 is located on the horizontal plate of the second gear mounting plate 40. An adjusting screw adjusting ring is provided between the second gear mounting plate 40 and the second driving bevel gear 1501 and below the second gear mounting plate 40. The two adjusting screw adjusting rings are respectively connected to the adjusting screw 15 through adjusting screw pins. An adjusting hole 35 is provided on the mounting frame 26. The lower end of the adjusting screw 15 is rotatably connected to the adjusting hole 35. A double sealing groove and an adjusting screw sealing ring 34 matching the double sealing groove are provided on the side wall of the adjusting screw 15. The adjusting screw sealing ring 34 abuts against the adjusting hole 35, so that the adjusting screw 15 can achieve radial sealing within the adjusting hole 35.

[0063] The limiting structure and connection method of the biosafety closed valve core 2: The biosafety closed valve core 2 is also provided with a guide arm 29 and a guide post 28, which serve as the limiting structure in the horizontal direction, restricting the biosafety closed valve core 2 to only move up and down along the guide post 28. The guide arm 29 has an opening in the middle, and the bottom surface of the ball nut 31 base is connected to the upper surface of the guide arm 29. The lower layer of the ball nut 31 extends below the guide arm 29 through the opening in the middle of the guide arm 29. In this embodiment, two guide posts 28 are provided, which are symmetrically distributed around the central axis of the housing 4. The upper and lower ends of the guide posts 28 are connected to the inner wall of the top surface of the air duct 1 and the support plate 32, respectively. The lifting mechanism screw 17 passes through the guide arm 29, and the guide arm 29 is perpendicular to the two guide posts 28, with each side of the guide arm 29 slidingly connected to one guide post 28 with damping. In this embodiment, the guide arm 29 has through holes at both ends, and damping material is provided along the inner edge of the through holes. The damping material includes elastic damping sleeves such as rubber or elastic surface mechanical retaining rings. The guide post 28 passes through the through holes on the guide arm 29, allowing the guide arm 29 to move up and down along the two guide posts 28. When the biosafety shut-off valve core 2 achieves different opening degrees, the guide arm 29 can remain at any position on the guide post 28 due to static friction between the guide arm 29 and the guide post 28, without adjustment force, thereby maintaining different heights of the ball nut 31 and realizing different positional relationships between the biosafety shut-off valve core 2 and the air duct 1.

[0064] The bottom edge of the enclosure 4 is connected to the room ceiling, and the entire enclosure 4 is located above the room ceiling. The bottom of the enclosure 4 is the installation location for the air vent cover 6 and the ventilation cover 23.

[0065] The ventilation cover 23 is provided with ventilation holes 24, hooks 22 and suction plates 25. When the ventilation cover 23 is installed at the bottom of the box 4, one side of the ventilation cover 23 is detachably hinged to the third pin 5 of the bottom side wall of the box 4 through the hooks 22, so that one side of the ventilation cover 23 can be flipped relative to the box 4. The other side of the ventilation cover 23 is provided with suction plates 25. The suction plates 25 are magnetically attracted to the magnetic bolts 12 provided on the bottom inner side wall of the box 4, so that the ventilation cover 23 is fastened to the bottom of the box 4.

[0066] Each inner side wall of the housing 4 is also provided with a fixing plate 9 with screw holes. When the bottom of the housing 4 is connected to the air vent cover 6, the threaded end of the fixing bolt 7 passes through the air vent cover 6 and connects with the screw hole of the fixing plate 9 with screw holes. A rubber cap 8 is provided between the bolt head of the fixing bolt 7 and the air vent cover 6. When the fixing bolt 7 is tightened, the rubber cap 8 seals the gap between the bolt 7 and the air vent cover 6. The gap between the air vent cover 6 and the housing 4 is filled with sealing silicone 11.

[0067] like Figure 3As shown in Figure 4, a pair of handles 10 are provided on the bottom surface of the air vent cover 6. The disinfection connector 19 is sealed through the air vent cover 6. The top of the disinfection connector 19 is a quick-connect threaded connector 21, and the bottom of the disinfection connector 19 is located on the outer side of the bottom surface of the air vent cover 6. The pressure testing connector 18 includes a male head that is sealed through the air vent cover 6 and a female head that is detachably connected to the bottom of the female head. In this embodiment, the female head is a quick-connect connector. The top of the male head is provided with a high-efficiency filter 20, and the bottom of the male head is located on the outer side of the bottom surface of the air vent cover 6. Depending on the disinfection usage scenario, the bottom of the ventilation valve 27 is closed and blocked by a ball valve, or the bottom of the ventilation valve 27 is opened by a ball valve and connected to the quick-connect threaded connector 21 through the lower dust generation pipeline.

[0068] During the disinfection process:

[0069] The bottom end of the disinfection connector 19 is connected to the air inlet of the circulating disinfection machine, and the upper end of the female connector of the pressure test connector 18 is inserted into the male connector of the pressure test connector 18. At the same time, the lower end of the female connector of the pressure test connector 18 is connected to the return port of the circulating disinfection machine, so that the disinfection gas after use can pass through the high-efficiency filter 20 and the pressure test connector 18 in a one-way manner from top to bottom and then return to the circulating disinfection machine.

[0070] The quick-connect threaded connector 21 at the top of the disinfection connector 19 is connected to one end of the lower dust-generating pipe, and the other end of the lower dust-generating pipe is connected to the bottom end of the vent valve 27. The top end of the vent valve 27 is connected to one end of the upper dust-generating pipe 13. The other end of the upper dust-generating pipe 13 is a movable end for discharging disinfection gas and extending to the top of the HEPA filter 14. The ball valve of the vent valve 27 is in the open state. External disinfection gas can be transported through the disinfection connector 19 sequentially through the lower dust-generating pipe, the vent valve 27, and the upper dust-generating pipe 13 to the top of the HEPA filter 14 and flow through the HEPA filter 14 from top to bottom for disinfection.

[0071] Alternatively, the quick-connect threaded connector 21 at the top of the disinfection connector 19 can be left unconnected, the ball valve of the ventilation valve 27 can be in the open state, and the bottom end of the ventilation valve 27 can be connected to the high-efficiency filter 20 through the air tube, so that the disinfection gas can pass through the HEPA filter 14 from bottom to top to disinfect the HEPA filter 14.

[0072] During the airtightness test:

[0073] The bottom of the disinfection connector 19 is connected to the pressure gauge, and the upper end of the female connector 18 is inserted into the male connector of the pressure testing connector 18, while the lower end of the female connector is connected to the air source.

[0074] During normal use, the ball valve of vent valve 27 is closed.

[0075] Example 2:

[0076] The usage methods of the air vent cover 6 and the ventilation cover 23 include the installation of the air vent cover 6, the airtightness test of the housing 4, the in-situ disinfection of the HEPA filter 14, and the installation and removal of the ventilation cover 23.

[0077] (1) Installation steps of air vent cover 6:

[0078] Step 1: Hold the handle 10 and lift it up so that the edge of the air vent cover 6 is embedded into the bottom of the box 4. At the same time, align the fixing bolt 7 with the fixing plate 9 with screw holes and screw the fixing bolt 7 into the screw hole of the fixing plate 9 with screw holes, so that the rubber cap 8 deforms and seals the gap between the air vent cover 6 and the fixing bolt 7.

[0079] Step 2: Fill the gap between the air vent cover 6 and the housing 4 with sealant silicone 11;

[0080] Step 3: After the sealing silicone 11 has solidified, the installation is complete.

[0081] (2) Test procedure for airtightness of enclosure 4:

[0082] Step 1: Close the biosafety airtight valve core 2: Rotate the adjusting screw 15, which drives the intermediate transmission rod 16, and then drives the lifting mechanism screw 17 to close the biosafety airtight valve core 2, thereby isolating the space between the box 4 and the air duct 1.

[0083] Step 2: Install the air vent cover 6;

[0084] Step 3: Insert the upper end of the female connector 18 into the male connector 18 to make the pressure testing connector 18 connected. Connect the air source from the lower end of the female connector 18, using air as the pressure testing gas. The pressure testing gas is filled into the chamber 4 from the bottom end of the pressure testing connector 18 upward through the high-efficiency filter 20. Connect the bottom end of the disinfection connector 19 to the pressure gauge. After filling with the set amount of pressure testing gas, turn off the air source and use the pressure gauge to observe the airtightness of the chamber 4.

[0085] Step 4: After the test is completed, disconnect the gas source. The pressure test gas will be discharged downward from the pressure test connector 18 into the housing 4. Finally, remove the female part of the pressure test connector 18 to seal the pressure test connector 18.

[0086] (3) HEPA filter 14 in-situ disinfection steps:

[0087] Step 1: Close the biosafety airtight valve core 2: Rotate the adjusting screw 15, which drives the intermediate transmission rod 16, and then drives the lifting mechanism screw 17 to close the biosafety airtight valve core 2, thereby isolating the space between the box 4 and the air duct 1.

[0088] Step 2: Set up the disinfection gas circulation path according to the requirements, and place several disinfection indicator tablets in different positions inside the box 4;

[0089] For the first type of disinfection gas circulation path, the main purpose is to disinfect the lower surface of the HEPA filter 14. The vent valve 27 is opened, and the bottom end of the vent valve 27 is connected to the high-efficiency filter 20 through the air pipe. The disinfection gas can enter the chamber 4 from the disinfection connector 19 and pass through the HEPA filter 14 from bottom to top. Then, it passes through the vent valve 27 and the high-efficiency filter 20 from top to bottom in sequence. Finally, the used disinfection gas is discharged downward to the return port of the circulating disinfection machine through the pressure test connector 18.

[0090] For the second type of disinfection gas circulation path, the lower surface of the HEPA filter 14 is mainly disinfected. The vent valve 27 is opened, and the top of the vent valve 27 is connected to one end of the upper dust-generating pipe 13. The other end of the upper dust-generating pipe 13 is a movable end that extends above the HEPA filter 14. The quick-connect threaded connector 21 at the top of the disinfection connector 19 is connected to one end of the lower dust-generating pipe, and the other end of the lower dust-generating pipe is connected to the bottom end of the vent valve 27. The disinfection connector 19 delivers external disinfection gas sequentially through the lower dust-generating pipe, the vent valve 27, and the upper dust-generating pipe 13 to the top of the HEPA filter 14, and then passes through the top of the HEPA filter 14 to the bottom of the HEPA filter 14. Finally, it is discharged downwards through the pressure test connector 18 to the return port of the circulating disinfection machine.

[0091] Step 3: Install the air vent cover 6;

[0092] Step 4: Connect the air supply port of the circulating sterilizer to the bottom of the sterilization connector 19, insert the upper end of the female connector of the pressure test connector 18 into the male connector of the pressure test connector 18, connect the bottom end of the female connector of the pressure test connector 18 to the return port of the circulating sterilizer, and then turn on the circulating sterilizer.

[0093] Step 5: After the circulation disinfection, disconnect the circulation disinfection machine from the disinfection connector 19 and the pressure testing connector 18 in sequence, remove the female part of the pressure testing connector 18, and remove the air vent cover 6.

[0094] 4) Installation and removal of ventilation cover 23:

[0095] Installation of ventilation cover 23: as follows Figure 8 As shown, the inner surface of the ventilation cover 23 is facing upwards, so that the edge of the ventilation cover 23 is embedded in the box 4, the hook 22 is hinged on the third pin 5, and the suction plate 25 is attached to the magnetic bolt 12.

[0096] Disassembly of ventilation cover 23: as follows Figure 8 As shown, by pulling down the vent 24, the suction plate 25 is disengaged from the magnetic bolt 12, and at the same time the hook 22 is separated from the third pin 5.

[0097] This invention can seal the chamber 4 and perform in-situ disinfection of the HEPA filter 14 in the chamber 4 and test the airtightness of the chamber 4 through the disinfection connector 19 and the pressure test connector 18. At the same time, during the testing of the enclosure structure in a high-level biosafety laboratory, it can isolate the biosafety laboratory and prevent contamination of facilities, equipment and environment.

[0098] It should be noted that the specific embodiments described in this invention are merely illustrative of the spirit of the invention. Those skilled in the art to which this invention pertains can make various modifications or additions to the described specific embodiments or use similar methods to substitute them, without departing from the spirit of the invention or exceeding the scope defined by the appended claims.

Claims

1. A HEPA filter housing for in-situ sealed sterilization, comprising a housing (4), characterized in that, The top of the housing (4) is connected to the bottom of the air duct (1), and the top of the air duct (1) is connected to the supply and exhaust air duct. The diameter of the top of the housing (4) is larger than the diameter of the bottom of the air duct (1). A biosafety shut-off valve core (2) is installed in the inner cavity at the connection between the housing (4) and the air duct (1). A ring-shaped mounting frame (26) is installed on the inner wall of the housing (4) below the biosafety shut-off valve core (2). A HEPA filter (14) is installed in the center of the mounting frame (26). The outer edge of the HEPA filter (14) is fixed. The gap between the inner ring of the mounting frame (26) and the outer edge of the HEPA filter (14) is sealed with sealant. A vent valve (27) is provided on the mounting frame (26). The top of the vent valve (27) is connected to one end of the upper dust-generating pipe (13). The other end of the upper dust-generating pipe (13) extends to the top of the HEPA filter (14). A ball valve is provided at the bottom of the vent valve (27). An air vent cover (6) or a ventilation cover (23) is installed at the bottom of the housing (4). The biosafety closed valve core (2) includes an upward converging inclined plate (201) and a bottom plate (202) connected to the bottom of the inclined plate (201). The outer edge of the bottom of the inclined plate (201) is provided with an inclined plate groove, which is filled with jelly glue (3). Two sealing grooves are provided at the bottom of the outer wall of the air duct (1). Each sealing groove is provided with a layer of air duct sealing ring (36). When the biosafety closed valve core (2) is closed, the bottom of the side wall of the air duct (1) is inserted with jelly glue (3), and the air duct sealing ring (36) abuts against the inner wall of the inclined plate groove. The bottom surface of the base plate (202) is connected to the top surface of the ball nut (31). The lifting mechanism screw (17) is located on the central axis of the box (4) and the lifting mechanism screw (17) passes through the bottom plate (202) and the ball nut (31) of the biosafety closed valve core (2) from top to bottom. The ball nut (31) and the lifting mechanism screw (17) form a ball screw structure. The intermediate transmission rod (16) is perpendicular to the lifting mechanism screw (17). The first active bevel gear (1601) sleeved at one end of the intermediate transmission rod (16) meshes with the first driven bevel gear (1701) sleeved at the bottom end of the lifting mechanism screw (17). The intermediate transmission rod (16) is perpendicular to the adjusting screw (15) and the second driven bevel gear (1602) sleeved at the other end of the intermediate transmission rod (16) meshes with the second active bevel gear (1501) sleeved at the top end of the adjusting screw (15). The ball nut (31) is a two-layer inverted stepped shape with a larger upper layer and a smaller lower layer. The upper layer is the base of the ball nut (31). A third pin hole in the horizontal direction is provided on the inner wall of the base of the ball nut (31). The spring pin (33) is set in the third pin hole. When the biosafety shut-off valve core (2) closes the air duct (1), the spring pin (33) is inserted into the first pin hole on the lifting mechanism screw (17). When the biosafety shut-off valve core (2) opens the air duct (1), the ball nut (31) moves downward and the spring pin (33) retracts the ball nut (31). A support plate (32) is provided under the base plate (202). A support plate connecting plate (30) is provided at each of the four corners of the support plate (32). The upper end face of each support plate connecting plate (30) is connected to the inner surface of the top wall of the box (4). A ventilation hole is provided in the central area of ​​the support plate (32). The horizontal plate and the vertical plate of the first gear mounting plate (37) are vertically connected and the horizontal plate of the first gear mounting plate (37) is fixed to the lower surface of the support plate (32). The horizontal plate and the vertical plate of the second gear mounting plate (40) are vertically connected and the horizontal plate of the second gear mounting plate (40) is vertically fixed to the inner surface of the side wall of the box (4). The vertical plate of the second gear mounting plate (40) is vertically fixed to the inner surface of the top wall of the box (4). Below the ball nut (31), the lifting mechanism screw (17) also passes through the horizontal plate of the first adjusting ring (38), the support plate (32) and the first gear mounting plate (37) in sequence. The first adjusting ring (38) is connected and fixed to the lifting mechanism screw (17) through the first pin (39). The lower part of the lifting mechanism screw (17) is provided with a shoulder (1702). The upper surface of the shoulder (1702) abuts against the lower surface of the horizontal plate of the first gear mounting plate (37). The first driven bevel gear (1701) is located below the horizontal plate of the first gear mounting plate (37). The two ends of the intermediate transmission rod (16) are rotatably connected to the vertical plate of the first gear mounting plate (37) and the vertical plate of the second gear mounting plate (40), respectively. The second driven bevel gear (1602) is above the horizontal plate of the second gear mounting plate (40). The second adjusting ring (1603) located on the intermediate transmission rod (16) between the first driving bevel gear (1601) and the vertical plate of the first gear mounting plate (37) is fixed to the intermediate transmission rod (16) by the second pin. The adjusting screw (15) is perpendicular to the horizontal plate of the second gear mounting plate (40) and is rotatably connected to the horizontal plate of the second gear mounting plate (40). The second driving bevel gear (1501) is located above the horizontal plate of the second gear mounting plate (40). An adjusting screw ring is provided between the second gear mounting plate (40) and the second driving bevel gear (1501) and below the second gear mounting plate (40). The two adjusting screw rings are respectively connected to the adjusting screw (15) through the adjusting screw pin. An adjusting hole (35) is provided on the mounting frame (26). The lower end of the adjusting screw (15) is rotatably connected to the adjusting hole (35).

2. The HEPA filter housing with in-situ sealed sterilization according to claim 1, characterized in that, The bottom surface of the ball nut (31) base is connected to the upper surface of the guide arm (29). The lower layer of the ball nut (31) extends to the bottom of the guide arm (29) through the opening in the middle of the guide arm (29). The guide columns (28) distributed symmetrically to the central axis of the box (4) are perpendicular to the guide arm (29) and are connected to it with damping sliding. The upper and lower ends of the guide columns (28) are connected to the inner wall of the top surface of the air duct (1) and the support plate (32) respectively. The lifting mechanism screw (17) passes through the guide arm (29).

3. The HEPA filter housing for in-situ sealed sterilization according to claim 2, characterized in that, When the ventilation cover (23) is installed at the bottom of the box (4), one side of the ventilation cover (23) is detachably hinged to the third pin (5) on the bottom side wall of the box (4) through the hook (22), and the other side of the ventilation cover (23) is provided with suction plate (25), which magnetically attracts the magnetic bolt (12) provided on the bottom inner side wall of the box (4).

4. The HEPA filter housing with in-situ sealed sterilization according to claim 3, characterized in that, Each inner side wall of the housing (4) is provided with a fixing plate (9) with screw holes at the bottom. When the bottom of the housing (4) is connected to the air vent cover (6), the threaded end of the fixing bolt (7) passes through the air vent cover (6) and connects with the screw hole of the fixing plate (9). A rubber cap (8) is provided between the bolt head of the fixing bolt (7) and the air vent cover (6). The gap between the air vent cover (6) and the housing (4) is filled with sealing silicone (11). The bottom surface of the air vent cover (6) is provided with The device is equipped with a pair of handles (10), a disinfection connector (19) that seals through the air vent cover (6), a quick-connect threaded connector (21) at the top of the disinfection connector (19), and the bottom of the disinfection connector (19) located on the outer side of the bottom surface of the air vent cover (6); the pressure testing connector (18) includes a male head that seals through the air vent cover (6) and a female head that is detachably connected to the bottom of the male head, a high-efficiency filter (20) is provided at the top of the male head, and the bottom of the male head is located on the outer side of the bottom surface of the air vent cover (6).

5. A method of using an in-situ sealed and sterilized HEPA filter housing, utilizing the in-situ sealed and sterilized HEPA filter housing as described in claim 4, characterized in that... Including the airtightness test procedure for the enclosure (4): Step 1: Close the biosafety shut-off valve core (2): Turn the adjusting screw (15) to close the biosafety shut-off valve core (2); Step 2: Install the air vent cover (6); Step 3: Insert the upper end of the female connector (18) into the male connector (18), connect the gas source from the lower end of the female connector (18), connect the bottom end of the disinfection connector (19) to the pressure gauge, and after filling with the set amount of pressure-measuring gas, turn off the gas source and observe the airtightness of the box (4). Step 4: After the test is completed, disconnect the gas source and discharge the pressure test gas downward from the pressure test connector (18) into the housing (4). Remove the female connector of the pressure test connector (18).

6. A method of using an in-situ sealed and sterilized HEPA filter housing, utilizing the in-situ sealed and sterilized HEPA filter housing as described in claim 4, characterized in that, Includes in-situ sterilization of HEPA filters (14): Step 1: Close the biosafety shut-off valve core (2): Turn the adjusting screw (15) to close the biosafety shut-off valve core (2); Step 2: Set up a disinfection gas circulation path and place several disinfection indicator tablets at different positions inside the box (4); For the first disinfection gas circulation path, open the ventilation valve (27) and connect the bottom of the ventilation valve (27) to the high-efficiency filter (20) through the air pipe; For the second type of disinfection gas circulation path, open the ventilation valve (27), the top of the ventilation valve (27) is connected to one end of the upper dust generation pipeline (13), the other end of the upper dust generation pipeline (13) is a movable end and extends to the top of the HEPA filter (14), the quick-connect threaded connector (21) at the top of the disinfection connector (19) is connected to one end of the lower dust generation pipeline, and the other end of the lower dust generation pipeline is connected to the bottom end of the ventilation valve (27); Step 3: Install the air vent cover (6); Step 4: Connect the air supply port of the circulating sterilizer to the bottom of the sterilization connector (19), insert the upper end of the female connector (18) into the male connector (18), connect the bottom end of the female connector (18) to the return port of the circulating sterilizer, and then turn on the circulating sterilizer. Step 5: After the circulation disinfection, disconnect the circulation disinfection machine from the disinfection connector (19) and the pressure test connector (18) in sequence, remove the female end of the pressure test connector (18), and remove the air vent cover (6).

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