Efficient water environment DNA intelligent collection device and collection method

Abstract

The application relates to a high-efficiency water environment DNA intelligent collecting device and a collecting method, and relates to the technical field of DNA collecting devices. The high-efficiency water environment DNA intelligent collecting device comprises a bottom plate, a water storage tank is arranged through the side wall of the bottom plate, a pushing block is slidably connected in the water storage tank, a sampling barrel is arranged on the side, away from the bottom plate, of the pushing block, a water inlet pipe is arranged on the side, away from the pushing block, of the sampling barrel, the water inlet pipe is communicated with the inside of the sampling barrel, an electromagnetic valve is arranged on the water inlet pipe, a first water pump is arranged on the side, away from the sampling barrel, of the bottom plate, the water inlet end of the first water pump is extended into the water environment through a pipeline, the water outlet end of the first water pump is connected to the side wall of the water storage tank through a pipeline and is communicated with the inside of the water storage tank, a second water pump is arranged on the side, away from the sampling barrel, of the bottom plate, the water inlet end of the second water pump is extended into the inside of the water storage tank through a pipeline, and the water outlet end of the second water pump is extended into the water environment through a pipeline. The application has the effect of improving the collecting efficiency of the collecting device.

Description

Technical Field

[0001] This application relates to the field of DNA collection device technology, and in particular to a highly efficient intelligent DNA collection device and method for aquatic environments. Background Technology

[0002] Environmental DNA refers to DNA contained in tissues or cells shed by organisms in the environment. By collecting environmental DNA samples and using existing high-throughput genetic sequencing analysis methods, it is possible to detect the species that have appeared in the surveyed area over a certain historical period. Environmental DNA technology is an efficient, low-cost, and environmentally friendly technique. Ecological monitoring based on environmental DNA can ensure the collection of effective samples while avoiding damage and disturbance to biological habitats. This aligns with the initial goal of minimizing secondary pollution in environmental damage investigations, and it is gradually becoming an important tool in environmental damage investigations.

[0003] Chinese Patent CN215985310U discloses an intelligent environmental DNA collection device for marine waters, comprising a remotely controlled boat, a processor, a power supply, and a remote control module. The remotely controlled boat is equipped with a pull rope and a pull rope retraction assembly. One end of the pull rope is connected to the retraction assembly, and the other end of the pull rope is equipped with a sampling bucket. The inside of the sampling bucket is a water storage chamber, and the top of the sampling bucket has a first opening communicating with the water storage chamber. A first solenoid valve is installed at the first opening. The remote control module is wirelessly connected to the processor. The retraction assembly, the power supply, the first solenoid valve, and the second solenoid valve are all electrically connected to the processor. In use, the remote control module sends a command to the processor, which controls the retraction assembly to work. The retraction assembly releases the pull rope, causing the sampling bucket to descend and sink into the water. When the sampling bucket descends to a certain depth, the remote control module sends a command to the processor, which controls the first solenoid valve to open. At this time, external water flows into the water storage chamber, completing the sampling operation.

[0004] Regarding the aforementioned technologies, the inventors believe that if the weight of the sampling bucket is less than the buoyancy of the aquatic environment, when the sampling bucket moves vertically into the aquatic environment to collect water, it is easy for the sampling bucket to be unable to move vertically under the action of buoyancy, that is, it is impossible for water in the aquatic environment to quickly enter the interior of the sampling bucket, thus resulting in low collection efficiency of the collection device. Summary of the Invention

[0005] To improve the collection efficiency of the collection device, this application provides an efficient intelligent DNA collection device and method for aquatic environments.

[0006] Firstly, this application provides a highly efficient intelligent DNA collection device for aquatic environments, employing the following technical solution:

[0007] A highly efficient aquatic environment DNA intelligent collection device includes a base plate, a water storage tank penetrating through the side wall of the base plate, a pushing block slidably connected inside the water storage tank, a sampling bucket disposed on the side of the pushing block away from the base plate, an inlet pipe disposed on the side of the sampling bucket away from the pushing block, the inlet pipe communicating with the inside of the sampling bucket, and a solenoid valve disposed on the inlet pipe. A first water pump is disposed on the side of the base plate away from the sampling bucket, the inlet end of the first water pump extending into the aquatic environment through a pipe, and the outlet end of the first water pump connected to the side wall of the water storage tank and communicating with the inside of the water storage tank through a pipe. A second water pump is disposed on the side of the base plate away from the sampling bucket, the inlet end of the second water pump extending into the inside of the water storage tank through a pipe, and the outlet end of the second water pump extending into the aquatic environment through a pipe.

[0008] By adopting the above technical solution, when water needs to be collected from the aquatic environment, the first water pump is started. The inlet of the first water pump draws water from the aquatic environment through a pipeline and injects it into a storage tank. At this time, the push block moves along the inside of the storage tank towards the bottom of the aquatic environment as the water content in the storage tank increases. When the sampling bucket reaches the sampling position, the first water pump is turned off and the solenoid valve is opened, allowing water from the aquatic environment to flow into the sampling bucket. After the sampling bucket has finished collecting water, the solenoid valve is closed, and the second water pump draws water from the storage tank through a pipeline and injects it into the aquatic environment. At this time, the push block moves along the inside of the storage tank towards the bottom plate, thereby removing the sampling bucket from the aquatic environment. The above-mentioned limitation of the movement position of the sampling bucket by the storage tank ensures that the sampling bucket always enters the aquatic environment in a vertical direction, effectively improving the collection efficiency of the collection device.

[0009] Preferably, a collection membrane is provided on the side of the sampling bucket away from the push block.

[0010] As the sampling bucket moves toward the bottom of the aquatic environment, the water directly below the sampling bucket tends to spread outwards under the pressure of the sampling bucket. By adopting the above-mentioned technical solution, a collecting membrane is set on the side of the sampling bucket away from the pushing block. The collecting membrane is used to collect the water that spreads out during the movement of the sampling bucket, thereby improving the accuracy of the sampling bucket in collecting water from a designated location.

[0011] Preferably, a gravity block is provided on the side of the collecting membrane away from the sampling bucket.

[0012] By adopting the above technical solution, the setting of the gravity block increases the gravity of the collecting membrane, causing the collecting membrane to move in the vertical direction, improving the stability of the collecting membrane during the vertical movement process, and reducing the impact force of water on the collecting membrane.

[0013] Preferably, the collecting membrane has several connecting rings on the side away from the sampling bucket, and a pull rope is threaded through the interior of the connecting rings. Both ends of the pull rope pass through the side wall of the push block and are connected to a winding rod. The push block has a receiving cavity inside, and two winding rods are rotatably connected to the inner wall of the receiving cavity. The inner wall of the receiving cavity is provided with a driving member to drive the two winding rods to rotate.

[0014] By adopting the above technical solution, the driving component drives the two winding rods to rotate, thereby tightening the pull rope and closing the collecting membrane away from the sampling bucket, thus accelerating the flow of water from the aquatic environment into the sampling bucket.

[0015] Preferably, the driving component includes a drive motor, a dovetail block, a drive rod, two drive racks, and two drive gears. The inner wall of the receiving cavity has an embedding groove for the drive motor to be embedded. The output end of the drive motor is connected to the end of a winding rod. The inner wall of the receiving cavity has a dovetail groove for the dovetail block to slide. The drive rod is disposed on the side wall of the dovetail block. One drive rack is disposed at one end of the drive rod, and the other drive rack is disposed at the other end of the drive rod. The two drive racks and the two drive gears are correspondingly arranged and mesh with each other. The two drive gears and the two winding rods are correspondingly arranged, and the drive gears are disposed on the side wall of the winding rods.

[0016] By adopting the above technical solution, the drive motor is started, which drives the connected winding rod to rotate. The winding rod connected to the drive motor drives another winding rod to rotate through the drive rod, drive rack, and drive gear, thereby realizing the rotation of the two winding rods, and then tightening the pull rope through the winding rods.

[0017] Preferably, the outer wall of the sampling barrel is provided with an assist plate, the outer wall of the sampling barrel is provided with an abutment groove for the assist plate to be embedded, a connecting rod is fixedly embedded inside the assist plate, the sampling barrel is provided with a clearance groove for the connecting rod to be embedded, and the inner wall of the receiving cavity is provided with a rotating component for driving the connecting rod to rotate.

[0018] By adopting the above technical solution, the rotating component drives the connecting rod to rotate, and the connecting rod drives the auxiliary plate to rotate towards the side of the water inlet pipe, so that the water in the water environment moves towards the side of the sampling bucket, thereby further accelerating the influx of water into the sampling bucket.

[0019] Preferably, the rotating component includes a support plate, a connecting rod, and two sets of first bevel gears. The support plate is disposed on the inner wall of the receiving cavity, the connecting rod is rotatably connected to the inside of the support plate, and the end of the connecting rod away from the winding rod is inserted into the relief groove. One set of first bevel gears is used to connect the connecting rod and a winding rod, and the other set of first bevel gears is used to connect the connecting rod and the connecting rod.

[0020] By adopting the above technical solution, when the winding rod rotates, the winding rod drives the docking rod to rotate on the support plate through the first bevel gear set. The docking rod drives the connecting rod to rotate through the first bevel gear set. The connecting rod drives the auxiliary plate to rotate, so that when the collecting membrane achieves the closing function on the side away from the sampling bucket, the connecting rod synchronously drives the auxiliary plate to rotate towards the water inlet pipe, thereby reducing the consumption of electrical energy.

[0021] Preferably, the inlet end of the second water pump is connected to a telescopic pipe via a pipeline. An auxiliary ring is provided on the side wall of the telescopic pipe, and a moving rod is provided on the side wall of the auxiliary ring. A moving groove is provided on the side of the pushing block away from the collecting membrane for the end of the moving rod to slide. A fixing rod is provided on the side wall of the auxiliary ring, and a positioning frame is provided on the side of the pushing block away from the collecting membrane. A locking member is provided on the positioning frame for fixing the fixing rod inside the positioning frame.

[0022] By adopting the above technical solution, when the end of the moving rod is inserted into the inner wall of the moving groove and moves along the inner wall of the moving groove, the fixed rod rotates synchronously until the end of the fixed rod is inserted into the inside of the positioning frame. Then, the positioning frame and the fixed rod are fixed by the locking component so that the end of the telescopic tube is fixed to the surface of the pushing block. Thus, when the collection device finishes collecting, water inside the water storage tank can be quickly extracted through the telescopic tube.

[0023] Preferably, the locking component includes a rotating rod, a locking plate, a torsion spring, a push plate, a mounting rod, and an assist rod. The rotating rod is rotatably connected to the inside of the positioning frame and is fixedly embedded inside the locking plate. The torsion spring is sleeved on the side wall of the rotating rod, with one end connected to the positioning frame and the other end connected to the locking plate. The push plate is slidably connected to the inside of the positioning frame. The mounting rod is located on the side of the push plate away from the push block. The positioning frame has a sliding hole for the mounting rod to move. The assist rod is located on the end of the mounting rod away from the push plate.

[0024] By adopting the above technical solution, when the moving rod slides along the inner wall of the moving groove, the fixed rod moves synchronously until the end of the fixed rod is inserted into the inside of the positioning frame. Then, the elastic force of the torsion spring drives the rotating rod to rotate, so that the locking plate abuts against the end wall of the fixed rod, thereby improving the stability of the end of the fixed rod inserted into the positioning frame.

[0025] On the other hand, the collection method of the high-efficiency aquatic environment DNA intelligent collection device provided in this application adopts the following technical solution:

[0026] A method for collecting DNA from a highly efficient intelligent aquatic environment DNA collection device includes the following steps:

[0027] The first water pump draws water from the aquatic environment into the storage tank, causing the push block to extend vertically into the aquatic environment along the inside of the storage tank.

[0028] Once the sampling bucket is moved to the sampling position, the solenoid valve on the inlet pipe is opened; then, the two winding rods are driven to rotate by the drive component, so that the pull rope is tightened, thereby closing the side of the collecting membrane away from the sampling bucket; the rotating component drives the connecting rod to rotate, so as to rotate the assist plate, thereby accelerating the flow of water in the water environment through the inlet pipe into the sampling bucket.

[0029] The second water pump draws water from the storage tank into the aquatic environment, causing the pusher block to detach from the aquatic environment along the vertical direction inside the storage tank.

[0030] By adopting the above technical solution, the sampling bucket can be moved up and down in the water environment by pushing the block along the inside of the water tank, so that water in the water environment can quickly enter the inside of the sampling bucket, thereby improving the sampling efficiency of the sampling device.

[0031] In summary, this application includes at least one of the following beneficial technical effects:

[0032] 1. When water needs to be collected from the aquatic environment, the first water pump is started. The water inlet of the first water pump draws water from the aquatic environment through the pipeline and injects it into the water storage tank. At this time, the push block moves along the inside of the water storage tank towards the bottom of the aquatic environment as the water content in the water storage tank increases. When the sampling bucket reaches the sampling position, the first water pump is turned off and the solenoid valve is opened, allowing water from the aquatic environment to flow into the sampling bucket. After the sampling bucket has finished collecting water, the solenoid valve is closed, and the second water pump draws water from the water storage tank through the telescopic pipe and injects it into the aquatic environment. At this time, the push block moves along the side of the water storage tank towards the bottom plate, thereby removing the sampling bucket from the aquatic environment. The above-mentioned limitation of the movement position of the sampling bucket by the water storage tank ensures that the sampling bucket always enters the aquatic environment in a vertical direction to collect water, effectively improving the collection efficiency of the collection device.

[0033] 2. As the sampling bucket moves toward the bottom of the aquatic environment, the water directly below the sampling bucket is easily dispersed by the pressure of the sampling bucket. By adopting the above technical solution, a collecting membrane is set on the side of the sampling bucket away from the push block. The collecting membrane is used to collect the water dispersed during the movement of the sampling bucket, thereby improving the accuracy of the sampling bucket in collecting water at a designated location. Attached Figure Description

[0034] Figure 1 This is a schematic diagram of the overall structure of a high-efficiency aquatic environment DNA intelligent collection device in an embodiment of this application.

[0035] Figure 2 This is a cross-sectional view used in the embodiments of this application to illustrate the connection structure between the water storage tank and the push block.

[0036] Figure 3 This is a cross-sectional view used to illustrate the structure of the locking element in the embodiments of this application.

[0037] Figure 4 This is a cross-sectional view used in the embodiments of this application to illustrate the internal structure of the push block.

[0038] Figure 5 This is a cross-sectional view used to illustrate the structure of the driving component in the embodiments of this application.

[0039] Figure 6 This is a cross-sectional view used in the embodiments of this application to illustrate the connection structure between the assist plate and the sampling bucket.

[0040] Explanation of reference numerals in the attached figures:

[0041] 1. Base plate; 11. Water storage tank; 111. Limiting groove; 12. First water pump; 13. Second water pump; 2. Pushing block; 21. Receiving cavity; 211. Rewinding rod; 212. Embedding groove; 213. Abutment groove; 214. Dovetail groove; 22. Moving groove; 23. Positioning frame; 231. Sliding hole; 24. Limiting block; 3. Sampling bucket; 31. Water inlet pipe; 311. Solenoid valve; 32. Clearing groove; 4. Collecting membrane; 41. Mounting ring; 42. Connecting rope; 43. Gravity block; 44. Connecting ring; 45. Pull rope; 5. Driving component; 51. Drive motor; 52. Dovetail block; 53. Drive rod; 54. Drive rack; 55. Drive gear; 6. Booster plate; 61. Connecting rod; 62. Second bevel gear set; 621. Second driving bevel gear; 622. Second driven bevel gear; 7. Rotating component; 71. Support plate; 72. Connecting rod; 73. First bevel gear set; 731. First driving bevel gear; 732. First driven bevel gear; 8. Telescopic tube; 81. Auxiliary ring; 811. Moving rod; 812. Fixed rod; 9. Locking component; 91. Rotating rod; 92. Locking plate; 93. Torsion spring; 94. Push plate; 95. Mounting rod; 96. Booster rod. Detailed Implementation

[0042] The following is in conjunction with the appendix Figure 1-6 This application will be described in further detail.

[0043] Firstly, embodiments of this application disclose a highly efficient intelligent DNA collection device for aquatic environments. (Refer to...) Figure 1 and Figure 2 The sampling device includes a base plate 1, a water storage tank 11 is inserted through the upper surface of the base plate 1, a push block 2 is slidably connected to the inner wall of the water storage tank 11, a limit block 24 is glued to the side wall of the push block 2, a limit groove 111 for the limit block 24 to slide is opened in the vertical direction on the inner wall of the water storage tank 11, a sampling bucket 3 is glued to the side of the push block 2 away from the base plate 1, an inlet pipe 31 is integrally formed on the side of the sampling bucket 3 away from the push block 2, the inlet pipe 31 and the sampling bucket 3 are connected internally, and a solenoid valve 311 is installed on the inlet pipe 31.

[0044] Reference Figure 1 and Figure 2 A first water pump 12 is bolted to the side of the base plate 1 away from the sampling barrel 3. The inlet of the first water pump 12 extends into the water environment through a pipe, and the outlet of the first water pump 12 is connected to the side wall of the water storage tank 11 through a pipe and communicates with the interior of the water storage tank 11. To ensure that the push block 2 has a lifting function inside the water storage tank 11 when the first water pump 12 injects water into the water storage tank 11, a rubber gasket can be set on the inner wall of the water storage tank 11 to improve the sealing between the water storage tank 11 and the push block 2. In this embodiment, the push block 2, the limiting block 24, the sampling barrel 3, the water inlet pipe 31, and the solenoid valve 311 are all made of lightweight plastic so that when the sampling device is initially positioned in the water environment, the limiting block 24 is located at the top of the inner wall of the limiting groove 111.

[0045] Reference Figure 1 and Figure 2 After the staff fixes the base plate 1 to the riverbank or the side wall of the boat, the first water pump 12 is started. The first water pump 12 draws water from the water environment and injects it into the water storage tank 11. As the water volume inside the water storage tank 11 increases, the push block 2 moves along the inside of the water storage tank 11. The push block 2 drives the sampling bucket 3 to move synchronously, so that the sampling bucket 3 extends into the water environment. When the sampling bucket 3 reaches the location to be sampled, the first water pump 12 is turned off and the solenoid valve 311 is opened, so that the water in the water environment flows into the sampling bucket 3 through the water inlet pipe 31. Then the solenoid valve 311 is closed, so that the sampling bucket 3 stores the water in the water environment at the location to be sampled.

[0046] Reference Figure 1 and Figure 3A second water pump 13 is connected to the side of the base plate 1 away from the sampling bucket 3 via a screw ring. The inlet end of the second water pump 13 is connected to a telescopic pipe 8 via a pipe. In this embodiment, the telescopic pipe 8 is a corrugated flexible hose. The end of the telescopic pipe 8 away from the second water pump 13 extends into the interior of the water storage tank 11, and the outlet end of the second water pump 13 extends into the water environment via a pipe. An auxiliary ring 81 is glued to the side wall of the telescopic pipe 8, and a moving rod 811 is glued to the side wall of the auxiliary ring 81. The moving rod 811 has an L-shaped cross-section. A moving groove 22 for the moving rod 811 to slide is provided on the side of the push block 2 near the base plate 1. The moving groove 22 is an arc-shaped groove.

[0047] Reference Figure 3 The side of the push block 2 away from the sampling bucket 3 is glued to the positioning frame 23. The positioning frame 23 is slidably connected to the fixing rod 812. The fixing rod 812 has an L-shaped cross section and is glued to the side wall of the auxiliary ring 81. A locking element 9 is provided between the positioning frame 23 and the fixing rod 812. The locking element 9 includes a rotating rod 91, a locking plate 92, a torsion spring 93, a push plate 94, a mounting rod 95, and an assist rod 96. The rotating rod 91 is rotatably connected to the inside of the positioning frame 23 and is fixedly embedded in the inside of the locking plate 92 with glue. The torsion spring 93 is sleeved on the side wall of the rotating rod 91. One end of the torsion spring 93 is glued to the side wall of the positioning frame 23, and the other end of the torsion spring 93 is glued to the side wall of the locking plate 92. The push plate 94 is slidably connected to the inside of the positioning frame 23. The mounting rod 95 is glued to the side of the push plate 94 away from the push block 2. The positioning frame 23 has a sliding hole 231 for the mounting rod 95 to move. The assist rod 96 is glued to the end of the mounting rod 95 away from the push plate 94.

[0048] Reference Figure 3 When the telescopic tube 8 is damaged after prolonged use, hold and move the assist rod 96. The assist rod 96 drives the mounting rod 95 to move horizontally along the inner wall of the sliding hole 231. The mounting rod 95 drives the push plate 94 to move towards the side of the locking plate 92, so that the locking plate 92 is pushed by the push plate 94, causing the locking plate 92 to rotate until the upper surface of the locking plate 92 and the push block 2 are flush. Then rotate the fixing rod 812 so that the end of the fixing rod 812 is disengaged from the inside of the positioning frame 23. Finally, disengage the end of the moving rod 811 from the inner wall of the moving groove 22, so that the staff can replace the telescopic tube 8.

[0049] Reference Figure 4 and Figure 5The push block 2 has an internal cavity 21, and two take-up rods 211 are rotatably connected to the inner wall of the cavity 21. A drive component 5 is provided on the inner wall of the cavity 21. The drive component 5 includes a drive motor 51, a dovetail block 52, a drive rod 53, two drive racks 54, and two drive gears 55. The output end of the drive motor 51 is connected to the end of one of the take-up rods 211. An embedding groove 212 is provided on the inner wall of the cavity 21 for the drive motor 51 to be embedded. The two drive gears 55 and the two take-up rods 211 are correspondingly arranged to drive... Gear 55 is glued to the side wall of take-up rod 211. Two drive gears 55 and two drive racks 54 are correspondingly arranged, and the drive gears 55 and drive racks 54 mesh with each other. The two drive racks 54 are connected to the drive rod 53. Dovetail block 52 is glued to the side wall of drive rod 53. The inner wall of receiving cavity 21 is provided with dovetail groove 214 for dovetail block 52 to move. In the embodiment of this application, dovetail block 52, drive rod 53, drive rack 54, drive gear 55 and take-up rod 211 are all made of lightweight plastic material.

[0050] Reference Figure 2 and Figure 4 Two winding rods 211 are wound together with a pull rope 45. The end of the pull rope 45 passes through the side wall of the push block 2. In order to ensure that the push block 2 extends into the water environment and reduce the possibility of water inside the receiving cavity 21, a rubber pad can be set inside the push block 2 and at the point where the pull rope 45 moves to improve the sealing of the receiving cavity 21. The side wall of the pull rope 45 is fitted with several connecting rings 44, which are connected to the collecting membrane 4. In this embodiment, the collecting membrane 4 is a PVC membrane. The collecting membrane 4 is glued to the side of the sampling bucket 3 away from the push block 2. The side wall of the collecting membrane 4 is glued with several mounting rings 41, and a connecting rope 42 is wound around each mounting ring 41. A gravity block 43 is wound around the end of each connecting rope 42 away from the collecting membrane 4.

[0051] Reference Figure 2 and Figure 4 When the sampling bucket 3 is inserted into the water environment to collect water, the gravity block 43 increases the gravity of the collecting membrane 4, improves the stability of the collecting membrane 4 in the vertical direction in the water environment, and reduces the impact force of water on the collecting membrane 4.

[0052] Reference Figure 2 and Figure 4When the solenoid valve 311 is opened, water from the aquatic environment flows into the sampling bucket 3 through the inlet pipe 31, and the drive motor 51 is started. The drive motor 51 drives the connected winding rod 211 to rotate, and then drives the other winding rod 211 to rotate through the drive gear 55 and the drive rack 54. This causes the two winding rods 211 to rotate in opposite directions, thereby enabling the collecting membrane 4 to close on the side away from the sampling bucket 3, thereby increasing the water collection speed of the sampling bucket 3.

[0053] Reference Figure 4 The inner wall of the receiving cavity 21 is provided with a rotating component 7, which includes a support plate 71, a docking rod 72, and two sets of first bevel gear sets 73. The support plate 71 is glued to the inner wall of the receiving cavity 21. The docking rod 72 is rotatably connected to the inside of the support plate 71. The first bevel gear set 73 includes a first driving bevel gear 731 and a first driven bevel gear 732. One first bevel gear set 73 is used to connect the docking rod 72 and a winding rod 211. The first driving bevel gear 731 in one first bevel gear set 73 is glued to the side wall of the winding rod 211. The first driven bevel gear 732 in one first bevel gear set 73 is glued to the side wall of the docking rod 72. The winding rod 211 and the docking rod 72 are arranged vertically. The end of the docking rod 72 away from the winding rod 211 extends into the interior of the sampling barrel 3. A plurality of connecting rods 61 are provided at the end of the docking rod 72 away from the winding rod 211. In this embodiment, four connecting rods 61 are provided. The four connecting rods 61 are located on the same horizontal plane and form a square. All four connecting rods 61 are rotatably connected to the sampling bucket 3.

[0054] Reference Figure 4 Another first bevel gear set 73 is used to connect a docking rod 72 and a connecting rod 61. The docking rod 72 and the connecting rod 61 are perpendicular to each other, and the connecting rod 61 connected to the docking rod 72 is arranged parallel to the winding rod 211. The first driving bevel gear 731 in the other first bevel gear set 73 is glued to the side wall of the docking rod 72, and the first driven bevel gear 732 in the other first bevel gear set 73 is glued to the side wall of the connecting rod 61.

[0055] Reference Figure 6 A second bevel gear set 62 is provided between adjacent connecting rods 61. The sampling barrel 3 has a clearance groove 32 for the second bevel gear set 62 to be embedded. The second bevel gear set 62 includes a second driving bevel gear 621 and a second driven bevel gear 622. The second driving bevel gear 621 is glued to the side wall of one connecting rod 61, and the second driven bevel gear 622 is glued to the side wall of the other connecting rod 61.

[0056] Reference Figure 6Each connecting rod 61 has a support plate 6 fixedly fitted on its side wall with glue, and the outer side wall of the sampling bucket 3 has an abutment groove 213 for the support plate 6 to be embedded in.

[0057] Reference Figure 6 When the drive motor 51 drives the connected winding rod 211 to rotate, the winding rod 211 drives the connected docking rod 72 to rotate via the first bevel gear set 73. The docking rod 72 drives the connected connecting rod 61 to rotate via the first bevel gear set 73. The connecting rod 61 drives the remaining connecting rods 61 to rotate via the second bevel gear set 62. This causes all the assist plates 6 to rotate. As the collecting membrane 4 closes on the side away from the sampling tank 3, several assist plates 6 simultaneously move towards one side of the collecting membrane 4, further increasing the water collection speed of the sampling tank 3.

[0058] The implementation principle of the efficient aquatic environment DNA intelligent collection device in this application embodiment is as follows: the first water pump 12 is started, and the first water pump 12 draws water from the aquatic environment into the interior of the water storage tank 11. At this time, the push block 2 moves along the inner wall of the water storage tank 11 toward the bottom of the aquatic environment. When the sampling bucket 3 reaches the collection position, the first water pump 12 is turned off and the solenoid valve 311 is opened. Then the drive motor 51 is started, and the drive motor 51 drives the connected winding rod 211 to rotate. Then, through the drive gear 55 and the drive rack 54, the other winding rod 211 is driven to rotate, so that the side of the collecting membrane 4 away from the sampling bucket 3 is closed. At this time, the winding rod 211 connected to the drive motor 51 drives the connected docking rod 72 to rotate under the action of the first bevel gear set 73. The docking rod 72 drives the connected connecting rod 61 to rotate through the first bevel gear set 73. The connecting rod 61 drives the remaining connecting rods 61 to rotate through the second bevel gear set 62, so as to drive all the assist plates 6 to rotate, so as to accelerate the water in the water environment to flow into the sampling bucket 3, thereby improving the water collection speed of the sampling bucket 3 and thus improving the collection efficiency of the collection device.

[0059] On the other hand, this application also discloses a method for collecting DNA using a highly efficient intelligent aquatic environment DNA collection device, comprising the following steps:

[0060] S1. The first water pump 12 pumps water from the water environment into the water storage tank 11, causing the push block 2 to extend into the water environment along the vertical direction of the inner wall of the water storage tank 11. The push block 2 drives the sampling bucket 3 to move synchronously, so that the sampling bucket 31 moves to the position to be collected in the water environment.

[0061] S2. After the sampling bucket 3 moves to the sampling position, the solenoid valve 311 on the water inlet pipe 21 is opened; then the drive motor 51 drives the connected winding rod 211 to rotate, so that the drive gear 55 on the winding rod 211 rotates, and the drive rack 54 drives the meshing drive rack 54 to move, so that the drive rod 53 moves. At this time, another drive rack 54 moves, and the other drive rack 54 drives the other winding rod 211 to rotate through the meshing drive gear 55, so that the pull rope 45 is wound up. The membrane 4 is tightened, thus achieving a closing function on the side away from the sampling bucket 3. When the drive motor 51 drives the connected winding rod 211 to rotate, the winding rod 211 drives the docking rod 72 to rotate through the first bevel gear set 73. The docking rod 72 drives the connected connecting rod 61 to rotate through the first bevel gear set 73. The connecting rod 61 drives the remaining connecting rods 61 to rotate through the second bevel gear set 62, so as to realize the rotation of all the assist plates 6, thereby accelerating the water in the water environment to flow into the sampling bucket 3 through the inlet pipe 31.

[0062] S3. The second water pump 13 pumps water from the water storage tank 11 into the water environment, causing the push block 2 to leave the water environment in the vertical direction along the inner wall of the water storage tank 11.

[0063] The implementation principle of the collection method of the high-efficiency aquatic environment DNA intelligent collection device in this application embodiment is as follows: the first water pump 12 draws water from the aquatic environment into the water storage tank 11, thereby driving the push block 2 to move along the inside of the water storage tank 11 toward the bottom of the aquatic environment, thereby moving the sampling bucket 3 to the collection position. Then, with the help of the collecting membrane 4 and the assist plate 6, the water in the aquatic environment flows into the interior of the sampling bucket 3 through the inlet pipe 31, thereby increasing the collection speed of the sampling bucket 3 and thus improving the collection efficiency of the collection device.

[0064] The above are all preferred embodiments of this application, and are not intended to limit the scope of protection of this application. Therefore, all equivalent changes made in accordance with the structure, shape and principle of this application should be covered within the scope of protection of this application.

Claims

1. A high-efficiency intelligent DNA collection device for aquatic environments, comprising a base plate (1), characterized in that: A water storage tank (11) is inserted through the side wall of the base plate (1). A push block (2) is slidably connected inside the water storage tank (11). A sampling bucket (3) is provided on the side of the push block (2) away from the base plate (1). A water inlet pipe (31) is provided on the side of the sampling bucket (3) away from the push block (2). The water inlet pipe (31) and the sampling bucket (3) are connected internally. A solenoid valve (311) is provided on the water inlet pipe (31). A water inlet pipe (31) is provided on the side of the base plate (1) away from the sampling bucket (3). There is a first water pump (12), the inlet of the first water pump (12) extends into the water environment through a pipe, and the outlet of the first water pump (12) is connected to the side wall of the water storage tank (11) through a pipe and communicates with the inside of the water storage tank (11). A second water pump (13) is provided on the side of the bottom plate (1) away from the sampling bucket (3). The inlet of the second water pump (13) extends into the inside of the water storage tank (11) through a pipe, and the outlet of the second water pump (13) extends into the water environment through a pipe. A collection membrane (4) is provided on the side of the sampling bucket (3) away from the push block (2); A gravity block (43) is provided on the side of the collecting membrane (4) away from the sampling bucket (3); The collecting membrane (4) is provided with several connecting rings (44) on the side away from the sampling bucket (3). A pull rope (45) is threaded through the interior of the several connecting rings (44). Both ends of the pull rope (45) pass through the side wall of the push block (2) and are connected to the winding rod (211). The push block (2) is provided with a receiving cavity (21). The two winding rods (211) are rotatably connected to the inner wall of the receiving cavity (21). The inner wall of the receiving cavity (21) is provided with a driving member (5) to drive the two winding rods (211) to rotate. The driving component (5) includes a drive motor (51), a dovetail block (52), a drive rod (53), two drive racks (54), and two drive gears (55). The inner wall of the receiving cavity (21) is provided with an embedding groove (212) for the drive motor (51) to be embedded. The output end of the drive motor (51) is connected to the end of a winding rod (211). The inner wall of the receiving cavity (21) is provided with a dovetail groove (214) for the dovetail block (52) to slide. The drive rod (53) is provided with... On the side wall of the dovetail block (52), one of the drive racks (54) is disposed at one end of the drive rod (53), and another drive rack (54) is disposed at the other end of the drive rod (53). The two drive racks (54) and two drive gears (55) are disposed correspondingly, and the drive racks (54) and drive gears (55) mesh with each other. The two drive gears (55) and two winding rods (211) are disposed correspondingly, and the drive gears (55) are disposed on the side wall of the winding rods (211). The outer wall of the sampling barrel (3) is provided with an assist plate (6), and the outer wall of the sampling barrel (3) is provided with an abutment groove (213) for the assist plate (6) to be embedded. The inside of the assist plate (6) is fixedly embedded with a connecting rod (61), and the inside of the sampling barrel (3) is provided with a relief groove (32) for the connecting rod (61) to be embedded. The inner wall of the receiving cavity (21) is provided with a rotating part (7) for driving the connecting rod (61) to rotate. The rotating component (7) includes a support plate (71), a docking rod (72), and two sets of first bevel gears (73). The support plate (71) is disposed on the inner wall of the receiving cavity (21). The docking rod (72) is rotatably connected to the inside of the support plate (71). One end of the docking rod (72) away from the winding rod (211) is inserted into the relief groove (32). One set of first bevel gears (73) is used to connect the docking rod (72) and the winding rod (211), and the other set of first bevel gears (73) is used to connect the docking rod (72) and the connecting rod (61).

2. The high-efficiency aquatic environment DNA intelligent collection device according to claim 1, characterized in that: The inlet of the second water pump (13) is connected to a telescopic pipe (8) through a pipe. An auxiliary ring (81) is provided on the side wall of the telescopic pipe (8). A moving rod (811) is provided on the side wall of the auxiliary ring (81). A moving groove (22) for the end of the moving rod (811) to slide is opened on the side of the push block (2) away from the collecting membrane (4). A fixing rod (812) is provided on the side wall of the auxiliary ring (81). A positioning frame (23) is provided on the side of the push block (2) away from the collecting membrane (4). A locking member (9) for fixing the fixing rod (812) inside the positioning frame (23) is provided on the positioning frame (23).

3. The high-efficiency aquatic environment DNA intelligent collection device according to claim 2, characterized in that: The locking component (9) includes a rotating rod (91), a locking plate (92), a torsion spring (93), a push plate (94), a mounting rod (95), and an assist rod (96). The rotating rod (91) is rotatably connected to the inside of the positioning frame (23). The rotating rod (91) is fixedly embedded in the inside of the locking plate (92). The torsion spring (93) is sleeved on the side wall of the rotating rod (91). One end of the torsion spring (93) is connected to the side wall of the positioning frame (23), and the other end of the torsion spring (93) is connected to the side wall of the locking plate (92). The push plate (94) is slidably connected to the inside of the positioning frame (23). The mounting rod (95) is located on the side of the push plate (94) away from the push block (2). The positioning frame (23) has a sliding hole (231) for the mounting rod (95) to move. The assist rod (96) is located on the end of the mounting rod (95) away from the push plate (94).

4. The collection method of the high-efficiency aquatic environmental DNA intelligent collection device according to any one of claims 1-3, characterized in that: Includes the following steps: The first water pump (12) draws water from the water environment into the water storage tank (11), causing the push block (2) to extend into the water environment in the vertical direction inside the water storage tank (11); After the sampling bucket (3) is moved to the sampling position, the solenoid valve (311) on the water inlet pipe (31) is opened; then the two winding rods (211) are driven to rotate by the driving component (5) so that the pull rope (45) is tightened, thereby closing the side of the collecting membrane (4) away from the sampling bucket (3); the rotating component (7) drives the connecting rod (61) to rotate so as to realize the rotation of the assist plate (6), thereby accelerating the water in the water environment to flow into the sampling bucket (3) through the water inlet pipe (31); The second water pump (13) pumps water from the water storage tank (11) into the water environment, causing the push block (2) to leave the water environment along the vertical direction inside the water storage tank (11).

Images