Compositions and uses thereof

By using surfactants and reducing agents to treat natural antigens in Chlamydia pneumoniae detection, combined with heat sealing and alkaline buffer, the problems of magnetic bead agglutination and instability of test results between samples were solved, achieving stability and consistency of test results.

CN116381227BActive Publication Date: 2026-06-26AUTOBIO DIAGNOSTICS CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
AUTOBIO DIAGNOSTICS CO LTD
Filing Date
2023-04-21
Publication Date
2026-06-26

AI Technical Summary

Technical Problem

Existing test kits for detecting Chlamydia pneumoniae suffer from magnetic bead agglutination and large differences in test results between samples, leading to unstable test results.

Method used

Adding surfactants and reducing agents, such as DTT, CHAPS, and Sarkosyl, during the antigen coating process, combined with a 3-day heat-sealing process and the use of alkaline CB buffer as a sealing solution, can improve the magnetic bead aggregation state and enhance the contrast between positive and negative test results.

Benefits of technology

It effectively reduces the range of test results between different samples, improves the stability and consistency of test results, and ensures ease of use for customers.

✦ Generated by Eureka AI based on patent content.

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Abstract

The present application relates to the technical field of biological detection, in particular to a composition and application thereof. The present application provides a composition and application thereof in a process of coating a magnetic bead with a natural antigen of Chlamydia pneumoniae, and a Chlamydia pneumoniae IgG antibody detection kit (magnetic microparticle chemiluminescence method). In the process, a surfactant and a reducing agent are added in the antigen coating process to improve the contrast of the detection results, a heat sealing process for 3 days is adopted to reduce the result range between different samples, and CB alkaline buffer is used as a sealing liquid buffer to improve the aggregation problem of the magnetic beads.
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