A composition containing polyoxin and difenoconazole

Abstract

The present invention belongs to the technical field of pesticide compounding and relates to a composition containing polyoxin and difenoconazole. The present invention provides a composition containing polyoxin and difenoconazole, the active ingredients of which include a fungicide and a synergist; the fungicide is polyoxin and difenoconazole, and the synergist is farnesol. Within a certain ratio range, the composition performs excellently in preventing and treating plant fungal diseases, and is superior to the single dosage of each component. Further experiments of the present invention have found that the introduction of a synergist further improves the control effect of the composition on plant fungal diseases.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticide compounding and relates to a composition containing polyoxin and difenoconazole. Background Art

[0002] Polyoxin, a nucleoside antibiotic originally isolated from Streptomyces cocophylla var. aso, is currently widely used as a metabolite of Streptomyces aureochromogenes. Polyoxin is a mixture of 14 different homologues, A to N, with polyoxin B as the main active ingredient. It belongs to the polyoxinidine nucleoside agricultural antibiotic fungicide class and effectively inhibits the synthesis of chitin, a component of the fungal cell wall skeleton, preventing cell wall biosynthesis and leading to bacterial death. Polyoxin is a highly effective, low-toxic, and environmentally friendly pesticide, making it widely used in the prevention and control of important diseases of grain crops, specialty crops, fruits, and vegetables.

[0003] Difenoconazole belongs to the triazole fungicide class. It exhibits preventive, therapeutic, and eradicative effects in disease control and is resistant to rain erosion. Difenoconazole's mechanism of action is primarily to inhibit the biosynthesis of ergosterol in pathogenic cells, thereby disrupting the structure and function of the pathogen's cell membrane and causing fungal death. It is a relatively safe, highly effective, broad-spectrum, low-toxic, low-use fungicide with preventive, therapeutic, and protective properties, as well as systemic activity. It is widely used on fruit trees, vegetables, cereals, and horticultural crops to control diseases such as black spot, leaf spot, and powdery mildew. It is also frequently used as a seed treatment agent for wheat and corn to control diseases such as smut.

[0004] Farnesol, English name: (E, E)-Farnesol, CAS: 106-28-5, molecular formula: C 15 H 26 Farnesol, a bioactive fragrance and pharmaceutical intermediate, is widely found in nature. Research reports indicate that farnesol is primarily found in the essential oils of plant flowers, leaves, and seeds, particularly in some Chinese herbal medicines, where it is found at high concentrations. Farnesol is a key active ingredient in these herbs. Farnesol is a highly effective, low-toxic natural antibacterial, mildew-removing, and odor-removing chemical. Therefore, it is often included in the formulations of antiseptic liquid cleaners and various detergents. Farnesol is also used for mildew removal in ion exchange resins and for wood preservation and antibacterial properties.

[0005] Mixing different pesticides is a common method for enhancing crop resistance and improving crop quality. Screening pesticide formulations to identify optimal formulations can effectively improve actual results, promote crop growth and quality, reduce pesticide dosage, and lower costs. However, in most cases, the effects of pesticide combinations are additive, with synergistic effects rarely seen, especially those with highly pronounced synergistic effects and high co-toxicity coefficients. Currently, there are no reports on the combination of polyoxin, difenoconazole, and farnesol. Summary of the Invention

[0006] The purpose of the present invention is to provide a composition containing polyoxin and difenoconazole, which has excellent effects in preventing and controlling plant fungal diseases.

[0007] Based on the above purpose, the present invention provides a composition containing polyoxin and difenoconazole, the active ingredients of which include a fungicide and a synergist; the fungicide is polyoxin and difenoconazole, and the synergist is farnesol.

[0008] Those skilled in the art readily recognize that to improve the efficacy of agricultural compositions and prevent the development of drug resistance, multiple pesticides are often used in combination or in combination. However, due to the lack of clarity regarding the components and underlying mechanisms, it is difficult to predict whether the control effects of these combinations or combinations will be synergistic. The present inventors, through in-depth experimental research, have determined, based on comprehensive field efficacy assessments, that the agricultural compositions of the present invention exhibit excellent control effects against plant fungal diseases, surpassing the effects of a single agent.

[0009] Furthermore, the agricultural composition provided by the present invention comprises, by weight, 20 to 60 parts of polyoxin, 30 to 80 parts of difenoconazole, and 1 to 30 parts of farnesol.

[0010] Preferably, the agricultural composition provided by the present invention comprises, by weight, 36 to 43 parts of polyoxin, 43 to 65 parts of difenoconazole, and 10 to 20 parts of farnesol.

[0011] Furthermore, in the agricultural composition provided by the present invention, a microemulsion is prepared, which is composed, by mass, of 20 to 60 parts of polyoxin, 30 to 80 parts of difenoconazole, 1 to 30 parts of farnesol, 50 parts of DMF, 100 parts of xylene, 100 parts of phenethylphenol polyoxyethylene ether, 50 parts of agricultural milk 500#, and 1000 parts of water.

[0012] Depending on the actual application needs, the composition of the present invention can be prepared into one of the following dosage forms: aqueous emulsion, microemulsion, wettable powder, water-dispersible granules, etc., using methods known to those skilled in the art. The functional composition also contains adjuvants required for preparing pesticide formulations. The adjuvants can be one or a mixture of aqueous solutions, emulsifiers, wetting agents, stabilizers, dispersants, thickeners, pH regulators, defoamers, antifreeze agents, fillers, etc. The adjuvants described in the present invention can all be known substances, such as various adjuvants commonly used in pesticide formulations, and can vary depending on the specific situation.

[0013] The application method can be spraying, root irrigation, root soaking, seed soaking, pouring, swinging, broadcasting, furrow application, hole application, etc. Spraying is preferred for preventing and controlling fungal diseases, and the preferred formulation for preventing and controlling fungal diseases is microemulsion.

[0014] The composition of the present invention is mainly used for preventing and controlling plant fungal diseases, and the fungal diseases are preferably gray mold and anthracnose.

[0015] Compared with the prior art, the technical solution provided by the present invention has the following beneficial effects or advantages:

[0016] The present invention provides a composition containing polyoxin and difenoconazole, the active ingredients of which include a fungicide and a synergist; the fungicide is polyoxin and difenoconazole, and the synergist is farnesol. Within a certain ratio range, the composition performs excellently in controlling plant fungal diseases, outperforming the individual components used alone. Further experiments in the present invention have found that the inclusion of the synergist further enhances the control efficacy of the composition and can effectively mitigate the impact of fungicide use on crop rotation. DETAILED DESCRIPTION

[0017] The technical solutions of the present invention are described below with reference to the following embodiments; however, the present invention is not limited to the following embodiments.

[0018] Example 1

[0019] This example provides an indoor toxicity test of a composition containing polyoxin and difenoconazole against tomato leaf mold.

[0020] Tomato leaves infected with tomato leaf mold that had never been treated with pesticides in a greenhouse were collected, rinsed with clean water and then rinsed again with sterile water, kept moisturized at 20-25°C for 24 hours, and conidia were washed off with sterile water to prepare a spore suspension for later use.

[0021] Test drugs: polyoxin (purchased from Wuhan Tianhui Bioengineering Co., Ltd.), difenoconazole (purchased from Tai'an Jiangzhou Biotechnology Co., Ltd.), and farnesol (purchased from Chengdu Jiaye Biotechnology Co., Ltd.).

[0022] The mycelial growth rate inhibition method was used in accordance with the Guidelines for Indoor Pesticide Bioassays (Zhu Chunyu et al., 2006). The test agents were prepared as needed into a series of solutions with a mass concentration gradient. Under sterile conditions, 1 mL of each prepared solution was transferred to a 9-cm-diameter Petri dish. Then, 9 mL of melted PSA medium was added to each dish (with no drug added as a control). The solution and medium were thoroughly mixed. After the cultured pathogens were completely solidified, they were punched into cakes (5 mm in diameter) using a microporous punch. The cakes were picked from the edge of the colony and transferred to the culture medium at different concentrations. Four dishes were used for each treatment, and the results were repeated three times. The plates were placed in an artificial climate chamber and incubated at 28°C. Mycelial growth in the control was monitored until the control dish was full. The colony expansion diameter of each treatment was measured using a digital vernier caliper, and the inhibition rate was calculated. Inhibition rate (%) = (control colony diameter - treated colony diameter) / control colony diameter × 100%.

[0023] SPSS statistical analysis software was used to perform statistical analysis and calculate the EC of each drug. 50 The co-toxicity coefficient (CTC value) of the mixture was calculated according to Sun Yunpei's method. The test results are shown in Table 1.

[0024] Measured toxicity index (ATI) = (standard agent EC 50 / Test drug EC 50 )×100.

[0025] Theoretical toxicity index (TTI) = toxicity index of agent A × percentage of A in the mixture + toxicity index of agent B × percentage of B in the mixture.

[0026] Co-toxicity coefficient (CTC) = [measured toxicity index (ATI) of the mixture / theoretical toxicity index (TTI) of the mixture] × 100.

[0027] A co-toxicity coefficient (CTC) ≥ 120 indicates a synergistic effect; a co-toxicity coefficient (CTC) ≤ 80 indicates an antagonistic effect; and a co-toxicity coefficient (CTC) 80 < 120 indicates an additive effect.

[0028] Table 1, Indoor toxicity test

[0029]

[0030]

[0031] It can be seen from Table 1 above that within the given ratio range of the present invention, the ternary combination of polyoxin, difenoconazole and farnesol exhibits excellent synergistic effect, and the co-toxicity coefficient is greater than 140.

[0032] Example 2

[0033] This example provides a field test of a composition containing polyoxin and difenoconazole against cucumber anthracnose.

[0034] The experimental site is sandy soil with medium fertility, and the irrigation method is flooding. The test crop is cucumber, the variety is Jinyou 101, and the test object is anthracnose (Colletotrichurm lagenarium). The preparation method is as follows: a microemulsion is prepared, and the composition is calculated by mass: 20-60 parts of polyoxin, 30-80 parts of fenpropimorph, 1-30 parts of farnesol, 50 parts of DMF, 100 parts of xylene, 100 parts of phenylethylphenol polyoxyethylene ether, 50 parts of agricultural milk 500#, and 1000 parts of water. The experimental groups are shown in Table 2, and the area of ​​the plot is 16m2 in random block arrangement. 2 Repeat 4 times. The first application is during the fruit-bearing period. Anthracnose is a sporadic disease. The second application is done 1 week later. The third application is done 1 week after the second application. The spraying method is used, and the application rate is 1kg / hm2. 2 The dosage of active ingredients is shown in Table 2.

[0035] Cucumber anthracnose grading standard: Level 0 means no lesions; Level 1 means the lesion area accounts for less than 5% of the total leaf area; Level 3 means the lesion area accounts for 6% to 10% of the total leaf area; Level 5 means the lesion area accounts for 11% to 25% of the total leaf area; Level 7 means the lesion area accounts for 26% to 50% of the total leaf area; Level 9 means the lesion area accounts for more than 50% of the total leaf area.

[0036]

[0037]

[0038] Table 2, control effect of cucumber anthracnose

[0039]

[0040]

[0041] As shown in Table 2, the polyoxin, difenoconazole and farnesol ternary compound microemulsion prepared by the present invention has an excellent control effect on cucumber anthracnose. The control effect reached 82.97-94.38% 7 days after the third application, which is much higher than that of the single-dose group without adding each component.

[0042] As described above, the present invention can be better implemented. The above embodiments only describe the preferred implementation methods of the present invention and do not limit the scope of the present invention. Without departing from the design spirit of the present invention, various changes and improvements made to the technical solutions of the present invention by ordinary technicians in this field should fall within the protection scope determined by the present invention.

Claims

1. Use of a composition containing polyoxin and difenoconazole in preventing and treating plant fungal diseases, characterized in that: The fungal diseases are leaf mold and anthracnose; The composition consists of 20-60 parts of polyoxin, 30-80 parts of difenoconazole and 1-30 parts of farnesol in parts by mass.

2. The use according to claim 1, characterized in that The composition consists of 36-43 parts of polyoxin, 43-65 parts of difenoconazole and 10-20 parts of farnesol in parts by mass.

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