Preparation of a novel recognition element full-length recombinant antibody of thiacloprid and application thereof in time-resolved fluorescent test strip

By preparing full-length recombinant antibodies and fluorescent microsphere probes based on mammalian expression systems, and combining them with time-resolved fluorescent test strips, the problem of rapid and sensitive detection of thiamethoxam residues in food was solved, achieving high sensitivity and wide applicability.

CN116535516BActive Publication Date: 2026-07-24JIANGNAN UNIV
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Patent Information

Application Number
CN202310443405.1
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2023-04-21
Publication Date
2026-07-24
Estimated Expiration
2043-04-21

AI Technical Summary

Technical Problem

Existing technologies are insufficient for the rapid, sensitive, and accurate detection of thiamethoxam residues in food, especially in fruits and vegetables. Furthermore, traditional instrumental methods are costly and unsuitable for on-site testing.

Method used

Full-length recombinant antibodies were prepared using a mammalian expression system. Fluorescent microspheres were used to replace traditional colloidal gold to prepare thiamethoxam hapten, complete antigen, and fluorescent probes. These were then used for immunochromatographic detection in conjunction with time-resolved fluorescent test strips.

Benefits of technology

It enables rapid quantitative analysis of thiamethoxam with a detection limit of 0.003 ng/mL, high sensitivity, strong specificity, wide detection range, stability and reliability, and high sample recovery rate, making it suitable for on-site testing.

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Abstract

The application discloses a novel thiacloprid recognition element full-length recombinant antibody preparation and application thereof in time-resolved fluorescent test paper strips, and belongs to the technical field of molecular biology and time-resolved immunological analysis rapid detection. The application provides a preparation method of thiacloprid hapten and complete antigen, and sequences of a thiacloprid full-length antibody heavy chain and light chain, wherein the amino acid sequence of the antibody heavy chain variable region is shown as SEQ ID NO. 8, and the amino acid sequence of the antibody light chain variable region is shown as SEQ ID NO. 6. The method for detecting thiacloprid by the test paper strip is simple, rapid, stable, reliable and high in sensitivity, is suitable for screening and on-site monitoring of a large number of samples, and has a good commercial application prospect.
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