Use of microRNA derived from palm charcoal or combinations thereof in the preparation of a hemostatic medicament
By identifying and enriching ath-miR159a, cpa-miR159b, and ath-miR166a-3p in palm charcoal, and targeting the 3'-UTR of ADRA2C and t-PA, the unknown mechanism of palm charcoal's hemostatic synergistic effect was resolved, achieving a significant hemostatic effect.
CN116726038BActive Publication Date: 2026-04-17NANJING UNIV
2 Cites 0 Cited by
Patent Information
- Application Number
- CN202210214376.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2022-03-04
- Publication Date
- 2026-04-17
- Estimated Expiration
- 2042-03-04
AI Technical Summary
Technical Problem
Existing technologies cannot explain the mechanism by which palm charcoal enhances hemostasis after charring, and it is unknown whether palm charcoal contains miRNA and its function.
Method used
The three microRNAs ath-miR159a, cpa-miR159b, and ath-miR166a-3p were enriched in palm charcoal. By targeting the 3'-UTR of ADRA2C and t-PA, the expression of the corresponding genes was inhibited, thereby enhancing the hemostatic effect.
Benefits of technology
The miRNA content in palm charcoal was significantly higher than that in the raw product. ath-miR159a and cpa-miR159b bound ADRA2C, while ath-miR166a-3p inhibited t-PA, promoting vasoconstriction and hemostasis, and enhancing the hemostatic effect.
✦ Generated by Eureka AI based on patent content.
Abstract
This invention discloses the application of microRNAs or combinations thereof derived from palm charcoal in the preparation of hemostatic drugs. The microRNAs derived from palm charcoal are selected from any one of ath-miR159a, cpa-miR159b, and ath-miR166a-3p. This study identified miRNAs in palm charcoal and clarified the changes in miRNAs after palm is processed into charcoal drugs. ath-miR159a and cpa-miR159b in palm charcoal can bind to the 3'-UTR of ADRA2C. ath-miR166a-3p in palm charcoal can target and inhibit the expression and secretion of t-PA in HUVEC cells.
Need to check novelty before this filing date? Find Prior Art