A method for inhibiting Vibrio parahaemolyticus in small shed shrimp breeding ponds

Abstract

The invention discloses a method for inhibiting Vibrio parahaemolyticus in a small shed shrimp breeding pond, belongs to the field of aquaculture, and discloses a method for inhibiting Vibrio parahaemolyticus in a small shed shrimp breeding pond. The method comprises the following steps: S1, laying: a small-pore plastic net is laid flat on the inner wall of the small shed pond, and a turbidity sensor, a pH sensor, two water pumps, an oxygenation pump, and a Mitsubishi controller are respectively arranged on the pond; S2, disinfection: a disinfectant is formed by dissolving a tangning powder in clean water, and the disinfectant is evenly sprayed into the pond, and then a water pump is used to extract well water from a deep well and pump it into the pond to form breeding pond water; S3, seedling release: an iron basin is taken, sufficient well water is poured into the basin, and a certain amount of shrimp seedlings are scooped up with a net bag and put into the water of the iron basin for washing, and after washing, the shrimp seedlings are put into the water body of the pond; S4, oxygenation: the disinfectant and the improver can be separated from the shrimp seedlings, and the disinfectant and the improver can be directly acted on the inner wall of the pond, so as to reduce the blank area of ​​antibacterial in the pond.

Description

Technical Field

[0001] The invention relates to the field of aquaculture, and more particularly to a method for inhibiting Vibrio parahaemolyticus in a small shed shrimp breeding pond. Background Art

[0002] Small shed shrimp farming refers to building small sheds in designated areas to cultivate white shrimp by utilizing the characteristics of the small sheds, such as four-season heat preservation, rain, snow and strong wind resistance, etc., to resist the influence of adverse external environments. This can overcome environmental restrictions to a certain extent, reduce the geographical limitations of white shrimp farming, and facilitate the promotion of farming nationwide. Small sheds can be adjacent to each other, which is also convenient for farmers to carry out large-scale farming and shorten the journey of workers to different small sheds;

[0003] During the breeding process, Vibrio parahaemolyticus brought in with shrimp fry or other infectious channels adheres to feed, leftover bait, organic debris, and dead algae to continue to reproduce. After the shrimp eats them, it is very easy to get food poisoning from Vibrio parahaemolyticus. The main symptoms are: the shrimp eats a small amount or no food, the jejunum is empty, the liver is swollen, the hepatopancreas is obviously atrophied, the shrimp body is not red (it becomes more transparent), the infected shrimp swims less in the water, and is often seen lying on the edge of the pond, with significantly reduced vitality, and occasionally there is a phenomenon of digging mud and sand;

[0004] In the existing small shed shrimp farming pond, Bacillus parahaemolyticus is sprinkled into the pond as an antibacterial agent to inhibit the pathogen. However, the antibacterial agent in the pond is not convenient to stir evenly, and it is difficult to cover the organic matter at the bottom of the pond. Vibrio parahaemolyticus is easily left on the organic matter, resulting in the problem of antibacterial blank areas. Therefore, we propose a method for inhibiting Vibrio parahaemolyticus in small shed shrimp farming ponds. Summary of the invention

[0005] Technical issues to be solved

[0006] In view of the problems existing in the prior art, the purpose of the present invention is to provide a method for inhibiting Vibrio parahaemolyticus in small-shed shrimp farming ponds, which can separate the disinfectant and the improver from the shrimp seedlings, directly act on the inner wall of the pond, and reduce the antibacterial blank area of ​​the pond.

[0007] Technical Solution

[0008] To solve the above problems, the present invention adopts the following technical solutions.

[0009] A method for inhibiting Vibrio parahaemolyticus in a small shed shrimp breeding pond, the method comprising the following steps:

[0010] S1. Laying: lay the small-hole plastic net flat on the inner wall of the small shed pond, and set the turbidity sensor, pH sensor, two water pumps, oxygen pump, and Mitsubishi controller on the pond respectively;

[0011] S2. Disinfection: Dissolve Tangning powder in clean water to form a disinfectant, spray it evenly into the pond, and then use a water pump to draw water from a deep well into the pond to form aquaculture pond water;

[0012] S3, stocking: take an iron basin, pour in enough well water, then use a net bag to scoop up a certain amount of shrimp fry and put them into the water of the iron basin for washing. After washing, put the shrimp fry into the water of the pond;

[0013] S4, oxygenation: Use a controller to control the oxygenation pump to suck air within a specified period of time and pump it into the pond along the porous pipe to increase the oxygenation of the water;

[0014] S5, watering: Use a pH sensor to monitor the pH value of the pond water in real time. Until the pH value exceeds the specified range, the controller controls a water pump to extract well water from the well and pump it into one end of the corresponding pond, and controls another water pump to extract dirty water from the other end of the corresponding pond and discharge it;

[0015] S6. Pond replacement: Use a turbidity sensor to monitor the turbidity of the pond water in real time until the turbidity exceeds a specified range, prepare a clean pond adjacent to the pond, remove the shrimp fry in the pond, and pour them into the clean pond;

[0016] S7. Bottom modification: After all the shrimp fry are scooped out of the pond, use a water pump to drain the dirty water. The organic matter in the dirty water falls on a small-pore plastic net, which is then pulled up from the four corners to drive the organic matter out of the pond and move it away. The small-pore plastic net is placed on flat ground and rinsed with a water gun. It is washed three times with detergent, hot water, and clean water respectively to make the small-pore plastic net clean and reusable. Then, use 55% potassium persulfate composite powder with disinfectant effect as an improvement agent, and spray the improvement agent evenly into the pond with a pneumatic pesticide sprayer to eliminate microorganisms and improve the pond environment. The disinfectant and improvement agent can be separated from the shrimp fry and act directly on the inner wall of the pond to kill the hemolytic Vibrio on the organic matter as much as possible and reduce the antibacterial blank area of ​​the pond.

[0017] Furthermore, in step S1, the output ends of the two water pumps are installed with water pipes through joints, and one end of the water pipes extends into the interior of the pond. The water inlet end of one of the water pumps is connected to a hose through a joint, and one end of the hose extends into the deep well for pond water intake, and the other water pump is used for pond drainage.

[0018] Furthermore, in step S2, the disinfectant has a concentration of 5% and is evenly sprayed into the pond by a pneumatic pesticide sprayer at a spraying rate of 50-100 g / m 2 , which can effectively oxidize pond organic matter at low concentrations without damaging plastic nets.

[0019] Furthermore, in step S3, the net bag is continuously shaken up and down to drive the shrimp fry to sway in the water of the iron basin. Under the action of inertia, the distance between the shrimp fry is increased to avoid mutual obstruction.

[0020] Furthermore, in step S4, the breeding staff holds a bamboo pole and pushes the porous tube along the lower wall of the pond, and changes the pressurization position according to a cycle of once a day, so that the pond water is evenly oxygenated without squeezing the shrimp fry.

[0021] Furthermore, in step S5, one end of the two water pipes extending into the pond is fixed with a plastic cage through a clamp, so that water can flow along the holes of the plastic cage, while the shrimp fry are blocked by the plastic cage to prevent the water pipes from sucking the shrimp fry into them.

[0022] Furthermore, in step S6, the farmer uses a net bag to fish out the shrimp fry in the pond, puts them into a bucket filled with well water, washes them for 30-60 seconds, and then pours them into a clean pond.

[0023] Furthermore, in step S6, dirty water in the pond is pumped out by a water pump until the shrimp fry are about to emerge.

[0024] Furthermore, in step S6, pure compound bacteria powder is sprinkled into the clean pond, wherein the pure compound bacteria powder contains bacteriophage, Bdellovibrio bacteriovorus, photosynthetic bacteria, lactic acid bacteria and Bacillus.

[0025] Furthermore, in step S7, the small-pore plastic net is placed on a flat ground and rinsed with a water gun, and is washed three times with detergent, hot water, and clean water respectively, so that the small-pore plastic net becomes clean and can be reused.

[0026] Beneficial Effects

[0027] Compared with the prior art, the advantages of the present invention are:

[0028] When this scheme is working, through the steps of laying, disinfection, stocking, oxygenation, watering, changing the pond and modifying the bottom, emergency rescue of shrimp fry is carried out when the pond environment deteriorates, the pond environment is improved, the disinfectant and the improver can be separated from the shrimp fry, and directly act on the inner wall of the pond, killing the hemolytic Vibrio on the organic matter as much as possible, and reducing the blank area of ​​antibacterial in the pond. In this scheme, take an iron basin, pour in enough well water, and then use a net bag to scoop up a certain amount of shrimp fry and put them into the water of the iron basin, wash for 0.5-1min, wash off the high salt water adhering to the shrimp fry, and constantly shake the net bag up and down to drive the shrimp fry to shake in the water of the iron basin. Under the action of inertia, the distance between the shrimp fry is increased to avoid mutual obstruction. (3) When this scheme is aerating, the breeding staff holds a bamboo pole and pushes the porous tube along the lower wall of the pond, changing the pressurization position according to a cycle of once a day, so that the pond water is oxygenated evenly and the shrimp fry will not be squeezed.

[0029] (4) In this scheme, the pond water shows a trend of eutrophication until the turbidity exceeds the specified range. A clean pond is prepared adjacent to the pond. The aquaculture personnel use a net bag to scoop out the shrimp fry in the pond and put them into a bucket filled with well water. They wash them for 30-60 seconds to wash away the organic matter adhering to the shrimp fry, and then pour them into the clean pond.

[0030] (5) In this scheme, the small-pore plastic net is placed on a flat surface and rinsed with a water gun. It is cleaned three times with detergent, hot water, and clean water respectively, so that the small-pore plastic net becomes clean and can be reused. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 The figure is a flow chart of the method of the present invention. DETAILED DESCRIPTION

[0032] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention; it is obvious that the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments, and all other embodiments obtained by ordinary technicians in this field based on the embodiments of the present invention without making creative work are within the scope of protection of the present invention.

[0033] Example:

[0034] See also Figure 1 , a method for inhibiting Vibrio parahaemolyticus in a small shed shrimp farming pond, the method comprising the following steps:

[0035] S1. Laying: lay a small-hole plastic net flat on the inner wall of the small shed pond. The pore size of the plastic net is smaller than the diameter of organic matter such as feed. The turbidity sensor and pH sensor of model Y510-B are installed inside the pond through flange plates. The turbidity sensor and pH sensor both work under DC9-24V conditions, and the flange plates are fixedly connected to the edge of the pond by bolts. Two water pumps are fixedly installed at both ends of the pond by bolts. The output ends of the two water pumps are installed with water pipes through joints, and one end of the water pipes extends into the pond. The water inlet end of one of the water pumps is connected with a hose through a joint, and one end of the hose extends into the deep well for pond water inlet. The other water pump is used for pond drainage. The aeration pump of model EXPLORER-65 is fixedly installed on one side of the pond by bolts. The output end of the aeration pump is installed with a porous tube through a joint, and one end of the porous tube extends into the pond. A Mitsubishi controller of model FX3U-32MT / ES-A is provided, which works under DC24V conditions and is used to control the turbidity sensor, pH sensor, two water pumps and aeration pump, and can be programmably debugged.

[0036] S2. Disinfection: Dissolve the commercially available Tangning powder in clean water to form a disinfectant with a concentration of 5%. Spray it evenly into the pond using a pneumatic pesticide sprayer at a spraying rate of 50-100 g / m 2 , which can effectively oxidize organic matter in the pond, with a low concentration, and will not damage the plastic net. Then use a water pump to draw water from the deep well and pump it into the pond to form the aquaculture pond water. The disinfectant can reduce harmful substances such as ammonia nitrogen, nitrite, hydrogen sulfide, etc. in the pond water, improve the pond water quality, stabilize the algae phase, and improve the water color;

[0037] S3, stocking: take an iron basin, pour enough well water into it, then use a net bag to pick up a certain amount of shrimp fry and put it into the water of the iron basin, wash it for 0.5-1min, wash off the high salt water adhering to the shrimp fry, and keep shaking the net bag up and down to drive the shrimp fry to shake in the water of the iron basin. Under the action of inertia, the distance between the shrimp fry is increased to avoid mutual blocking. After cleaning, use the net bag to put the shrimp fry into the water of the pond, repeat the above steps, and stock all ponds;

[0038] S4. Oxygenation: The controller controls the oxygenation pump to draw air within a specified time period and pump it into the pond along the porous pipe to increase the oxygen content of the water. Vibrio parahaemolyticus is an anaerobic bacterium. Increasing the oxygen concentration in the water can inhibit the growth of Vibrio parahaemolyticus. The aquaculture personnel hold a bamboo pole and push the porous pipe along the lower wall of the pond to change the pressurization position once a day to make the pond water evenly oxygenated without squeezing the shrimp seedlings.

[0039] S5, watering: at the same time, the pH value of the pond water is monitored in real time by a pH sensor, converted into an electrical signal and transmitted to the controller until the pH value exceeds the specified range. The controller controls a water pump to extract well water from the well and pump it into one end of the corresponding pond, and controls another water pump to extract dirty water from the other end of the corresponding pond and discharge it into the drainage ditch, gradually diluting the pond water, reducing the pH value of the water and softening it, so that the pH value and salinity of the water are reduced, which is not suitable for the growth of Vibrio parahaemolyticus. One end of the two water pipes extending into the pond is fixed with a plastic cage by a clamp, and water can flow along the holes of the plastic cage, while the shrimp fry are blocked by the plastic cage to prevent the water pipes from sucking the shrimp fry;

[0040] S6. Pond change: Use a turbidity sensor to monitor the turbidity of the pond water in real time, convert it into an electrical signal and transmit it to the controller. As the farmers put more bait and other organic matter into the water, the digestion capacity of the shrimp fry is limited, and the organic matter gradually increases. The pond water shows a trend of eutrophication until the turbidity exceeds the specified range. Prepare a clean pond adjacent to the pond, and the farmers use a net bag to fish out the shrimp fry in the pond, put them in a bucket filled with well water, wash them for 30-60 seconds, wash off the organic matter adhering to the shrimp fry, and then pour them into the clean pond. In the process of reducing cross-infection between adjacent ponds, a water pump is used to pump out dirty water from the pond until the shrimp fry are almost exposed, so as to maintain the water conditions required for the survival of the shrimp fry and facilitate the farmers to retrieve the shrimp fry. When the pond environment deteriorates, the shrimp fry can be rescued urgently. The composite bacterial pure powder containing bacteriophage, bacteriovorus, photosynthetic bacteria, lactic acid bacteria and bacillus is sprinkled into the clean pond. Probiotics are cultivated in the water to form a dominant population, so that the proportion of beneficial bacteria in the bacterial phase is dominant and the proportion of Vibrio is reduced.

[0041] S7. Bottom modification: After all the shrimp fry are scooped out of the pond, use a water pump to drain the dirty water. The organic matter in the dirty water falls on a small-pore plastic net, which is then pulled up from the four corners to drive the organic matter out of the pond and move it away. The small-pore plastic net is placed on flat ground and rinsed with a water gun. It is washed three times with detergent, hot water, and clean water respectively to make the small-pore plastic net clean and reusable. Then, use 55% potassium persulfate composite powder with disinfectant effect as an improvement agent, and spray the improvement agent evenly into the pond with a pneumatic pesticide sprayer to eliminate microorganisms and improve the pond environment. The disinfectant and improvement agent can be separated from the shrimp fry and act directly on the inner wall of the pond to kill the hemolytic Vibrio on the organic matter as much as possible and reduce the antibacterial blank area of ​​the pond.

[0042] The above is only a preferred specific implementation of the present invention; however, the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical solution and its improved conception within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A method for inhibiting Vibrio parahaemolyticus in a small shed shrimp breeding pond, characterized in that: The method comprises the following steps: S1. Laying: lay the small-hole plastic net flat on the inner wall of the small shed pond, and set the turbidity sensor, pH sensor, two water pumps, oxygen pump, and Mitsubishi controller on the pond respectively; S2. Disinfection: Dissolve Tangning powder in clean water to form a disinfectant with a concentration of 5%. Spray it evenly into the pond at a spraying rate of 50-100g / m 2 , and then use a water pump to draw water from the deep well and pump it into the pond to form the aquaculture pond water; S3, stocking: take an iron basin, pour in enough well water, then use a net bag to scoop up a certain amount of shrimp fry and put them into the water of the iron basin for washing. After washing, put the shrimp fry into the water of the pond; S4, oxygenation: Use a controller to control the oxygenation pump to suck air within a specified period of time and pump it into the pond along the porous pipe to increase the oxygenation of the water; S5, watering: Use a pH sensor to monitor the pH value of the pond water in real time. Until the pH value exceeds the specified range, the controller controls a water pump to extract well water from the well and pump it into one end of the corresponding pond, and controls another water pump to extract dirty water from the other end of the corresponding pond and discharge it; S6. Pond replacement: Use a turbidity sensor to monitor the turbidity of the pond water in real time until the turbidity exceeds a specified range, prepare a clean pond adjacent to the pond, remove the shrimp fry in the pond, and pour them into the clean pond; S7. Improve the bottom of the pond: After all the shrimp fry have been scooped out, use a water pump to drain the dirty water, then pull up the small-hole plastic net from the four corners to drive the organic matter out of the pond and remove it. Then, use 55% potassium persulfate compound powder as an improvement agent and spray the improvement agent evenly into the pond.

2. The method for inhibiting Vibrio parahaemolyticus in a small shed shrimp breeding pond according to claim 1, characterized in that: In step S1, the output ends of the two water pumps are both equipped with water pipes through joints, and one end of the water pipes extends into the pond. The water inlet end of one of the water pumps is connected to a hose through a joint, and one end of the hose extends into the deep well for pond water intake. The other water pump is used for pond drainage.

3. The method for inhibiting Vibrio parahaemolyticus in a small shed shrimp breeding pond according to claim 1, characterized in that: The disinfectant in step S2 has a concentration of 5% and is evenly sprayed into the pond by a pneumatic pesticide sprayer, 50-100 g / m 2 , effectively oxidizes pond organic matter at low concentrations without damaging plastic nets.

4. The method for inhibiting Vibrio parahaemolyticus in a small shed shrimp breeding pond according to claim 1, characterized in that: In step S3, the net bag is continuously shaken up and down to drive the shrimp fry to sway in the water of the iron basin. Under the action of inertia, the distance between the shrimp fry is increased to avoid mutual blocking.

5. The method for inhibiting Vibrio parahaemolyticus in a small shed shrimp breeding pond according to claim 1, characterized in that: In step S4, the breeding staff holds a bamboo pole and pushes the porous pipe along the lower wall of the pond to change the pressurization position once a day to evenly oxygenate the pond water without squeezing the shrimp fry.

6. The method for inhibiting Vibrio parahaemolyticus in a small shed shrimp breeding pond according to claim 1, characterized in that: In step S5, one end of the two water pipes extending into the pond is fixed with a plastic cage through a clamp, and water flows along the holes of the plastic cage, while the shrimp fry is blocked by the plastic cage to prevent the water pipes from sucking in the shrimp fry.

7. The method for inhibiting Vibrio parahaemolyticus in a small shed shrimp breeding pond according to claim 1, characterized in that: In step S6, the farmer uses a net bag to fish out the shrimp fry in the pond, puts them into a bucket filled with well water, washes them for 30-60 seconds, and then pours them into a clean pond.

8. The method for inhibiting Vibrio parahaemolyticus in a small shed shrimp breeding pond according to claim 1, characterized in that: In step S6, dirty water in the pond is pumped out by a water pump until the shrimp fry are about to emerge.

9. The method for inhibiting Vibrio parahaemolyticus in a small shed shrimp breeding pond according to claim 1, characterized in that: In the step S6, the pure powder of composite bacteria is sprinkled into the clean pond, wherein the pure powder of composite bacteria contains bacteriophage, bacteriovorus, photosynthetic bacteria, lactic acid bacteria and bacillus.

10. The method for inhibiting Vibrio parahaemolyticus in a small shed shrimp breeding pond according to claim 1, characterized in that: In step S7, the small-pore plastic net is placed on a flat ground and rinsed with a water gun, and is washed three times with detergent, hot water, and clean water respectively, so that the small-pore plastic net becomes clean and can be reused.

Images