A device and method for artificially cultivating tiger-striped squid in an indoor cement pool, cultivating, accelerating maturation and inducing spawning

Abstract

The present invention discloses a device and method for cultivating, maturing, and inducing spawning of tiger-striped squid broodstock artificially cultured in an indoor cement pool. The device relates to the field of aquaculture technology and comprises a culture pool and multiple spawning frames. The culture pool is provided with multiple water inlet pipes, a drain outlet is provided on the bottom of the culture pool, and the drain outlet is connected to a first drain pipe. A barrier cover is provided corresponding to the drain outlet, and the barrier mesh is evenly distributed on the surface of the barrier cover. The multiple spawning frames are used to be located within the culture pool. The purpose of the present invention is to provide a device and method for cultivating, maturing, and inducing spawning of tiger-striped squid broodstock artificially cultured in an indoor cement pool, which has high egg quality and a high hatchability.

Description

Technical Field

[0001] The invention belongs to the technical field of aquaculture, and in particular relates to a device and method for artificially cultivating tiger-striped squid in an indoor cement pool, cultivating parents, accelerating maturation and inducing spawning. Background Art

[0002] The tiger squid (Sepia pharaonis) belongs to the phylum Mollusca, order Sepioidea, family Sepiidae, genus Sepia. It is found in the coastal waters of the northwest Pacific and northern Indian Oceans, from nearshore to depths of 100 meters. Its large size (maximum carcass length 48 cm, weight up to 5 kg), tender meat, delicious flavor, and rich nutrition make it a highly economically valuable nearshore catch. Due to its high feed conversion rate, suitability for high-density aquaculture, strong disease resistance, and rapid growth (it can be marketed after 3-4 months of culture), it is a highly profitable cephalopod with great aquaculture potential.

[0003] All tiger squid seedlings available for aquaculture are hatched from fertilized eggs laid by wild populations in the South China Sea. Due to the limited and unstable number of fertilized eggs produced by wild populations each year, the number of seedlings available for cultivation is limited, making it difficult to meet the market demand for large-scale tiger squid farming. In recent years, in response to the growing popularity of tiger squid farming, relevant researchers have conducted research on fully artificial tiger squid seedling propagation technology. However, in these early studies, common problems have arisen, such as low overwintering survival rates of artificially farmed tiger squid broodstock, poor egg quality, and low (almost zero) hatching rates.

[0004] Existing technology includes an invention patent titled "Artificial Squid Spawning Bed," with publication number JP2003284445A. This invention provides an artificial squid spawning bed that uses a lightweight, easy-to-assemble plastic net, which can be maintained in shape using non-perishable fishing nets or ropes. This allows for easy installation and recovery of eggs comparable to those produced by spruce trees. However, this invention fails to guarantee egg quality and hatchability. Summary of the Invention

[0005] The purpose of the present invention is to provide a device and method for cultivating, accelerating maturation and inducing spawning of tiger-striped cuttlefish artificially cultured in an indoor cement pool with high egg quality and high hatching rate.

[0006] The technical solutions adopted by the present invention to achieve the above-mentioned purpose are:

[0007] A device for artificially cultivating tiger-striped squid in an indoor cement pool for breeding, accelerating maturation, and inducing spawning, comprising a breeding pool body and a plurality of spawning frames, wherein the breeding pool body is provided with a plurality of water inlet pipes, and a drainage outlet is provided on the bottom side of the breeding pool body, and the drainage outlet is connected to a first drainage pipe;

[0008] A barrier cover is provided at the drain outlet, and barrier meshes are evenly distributed on the surface of the barrier cover. Multiple spawning frames are used to be arranged in the breeding pond.

[0009] Through the overall design of a device for artificially breeding tiger-striped squid in an indoor cement pool for breeding parents, promoting maturation and inducing spawning, a plurality of water inlet pipes are arranged in the breeding pool to provide clean water rich in oxygen to the breeding pool, a drainage outlet is provided at the bottom of the breeding pool, and the drainage outlet is connected to a first drainage pipe. The end of the first drainage pipe is lower than the surface of the water in the breeding pool, so that dirt, leftover bait, feces and other debris that have settled to the bottom of the breeding pool can be discharged from the drainage outlet, thereby achieving cleaning of the breeding pool and improving breeding efficiency. The cleanliness of the water in the breeding pond can be improved to prevent eutrophication of the water in the breeding pond or the breeding of a large number of bacteria, which may lead to the death of the tiger-striped squid. A barrier cover is provided at the drain outlet, and barrier meshes are evenly distributed on the surface of the barrier cover. The size of the barrier mesh can be flexibly adjusted according to the size and age of the tiger-striped squid. The setting of the barrier cover can prevent the squid from being adsorbed on the plastic basket and being scratched and killed due to excessive drainage suction. The size of the barrier mesh can be adjusted to ensure the smooth discharge of debris such as leftover bait and feces at the bottom of the pond, while preventing the juvenile squid from escaping.

[0010] By setting up an spawning frame, when most of the tiger-striped squids mate, the spawning frame can be placed in the water to induce them to spawn quickly. Since fertilized eggs have a positive tendency towards the spawning behavior of the tiger-striped squid, as long as a small number of fertilized eggs are attached, a large number of paired tiger-striped squids will lay eggs on the spawning frame.

[0011] According to one embodiment of the present invention, a second shaft body is vertically provided on the top of the barrier cover, and an arc-shaped plate is provided on the top of the second shaft body. The arc-shaped plate is bowl-shaped, and the concave side of the arc-shaped plate faces the bottom side of the culture pond.

[0012] Through the above design, a certain distance can be formed between the arc plate and the barrier cover through the second axis. When the drain outlet in the barrier cover discharges sewage from the water in the breeding pond, the flow direction of the water around the barrier cover can be limited, so that the water flowing into the drain outlet can flow along the bottom of the breeding pond as much as possible, and the dirt deposited on the bottom of the breeding pond can be concentrated and effectively collected, avoiding the formation of water flows gathering in the direction of the barrier cover from all directions in the breeding pond, and avoiding the water flow from concentrating a large amount of water in the breeding pond in the direction of the drain outlet during sewage discharge, thereby preventing the tiger-striped squid from gathering in the center of the breeding pond under the guidance of the water body, which may cause a stress response.

[0013] Furthermore, the concave side of the curved plate is concentrically configured with multiple steps, each with an annular strip at its end. These concentric steps and strips can slow the water as it flows through, reducing the flow rate around the barrier shield beneath the curved plate. This prevents excessive flow from causing the tiger squid to converge toward the center of the pond, thereby reducing the likelihood of abrasions or stress reactions.

[0014] According to one embodiment of the present invention, a first shaft body is provided on the top of the arc-shaped plate, the first shaft body and the second shaft body are provided correspondingly, a connecting rod is provided on the top of the first shaft body symmetrically extending to both sides, and the axis of the connecting rod is provided horizontally with the top surface of the barrier cover;

[0015] There is at least one guard on both sides of the connecting rod, which includes a guard plate. A guard strip is arranged around the guard plate facing the bottom of the breeding pond. A guard rod is provided on the other side of the guard plate, and the guard rod is connected to the connecting rod.

[0016] Through the above design, the retaining strips provided on the retaining plate can protect the organisms and eggs, preventing them from moving toward the drain outlet during sewage discharge, thereby protecting the organisms and eggs. The retaining strips allow debris such as dirt, leftover bait, and feces to pass toward the drain outlet without affecting sewage discharge. At the same time, the retaining plate reduces the flow rate of the water around the spawning basket, thereby reducing the risk of eggs attached to the spawning basket falling due to the influence of the water flow. The first shaft can be configured to rotate or be fixed.

[0017] According to one embodiment of the present invention, the bottom side of the culture pond is inclined toward the drain outlet.

[0018] Through the above design, the leftover bait, feces and other debris on the bottom of the breeding pond can be gathered towards the drain outlet, thereby improving the drainage efficiency of the drain outlet for discharging dirt, leftover bait, feces and other debris.

[0019] Further, the entire bottom of the culture pond body is inclined at an angle of 2 to 5 degrees to the middle drain outlet. Further, the entire bottom of the culture pond body is inclined at an angle of 2 to 4 degrees to the middle drain outlet.

[0020] According to one embodiment of the present invention, the end of the first drain pipe houses a multi-section sleeve. This design allows for gradual removal of the sleeve during drainage, facilitating the discharge of debris and preventing the squid from becoming attached to the plastic basket due to a significant pressure difference between the inside and outside of the pipe, potentially resulting in abrasions or death.

[0021] Furthermore, the rear end of the sleeve is higher than the water surface in the culture pond. By the above design, the sleeve can be unfolded when the culture pond does not need to be drained, i.e., no drainage is achieved.

[0022] According to one embodiment of the present invention, a drainage component is further included. The drainage component includes a drainage cannula vertically arranged in the culture body, a plurality of drainage holes are provided on the upper portion of the drainage cannula, and a second drain pipe is connected to the lower end of the drainage cannula.

[0023] The above design enables the drainage assembly to achieve a water flow function. Specifically, the drainage assembly drains the water within the aquaculture pond through multiple drainage holes provided at the top of the drainage cannula. Specifically, the surface or upper water is discharged through the drainage holes into a second drain pipe, the end of which is lower than the surface of the water within the aquaculture pond, and is then discharged through the second drain pipe. Because the drainage holes are located at the top of the drainage cannula, air can flow into the top of the drainage pipe, thereby improving the water intake efficiency of the drainage holes and facilitating the rapid flow of surface dirt out of the water along with the running water.

[0024] Furthermore, a first pump body is provided within the aquaculture tank, located on the upper surface or upper layer of the water within the aquaculture tank. The first pump body is configured to guide the surface water within the aquaculture tank toward the drainage cannula. This design allows the surface water to flow toward the drainage cannula, improving the efficiency of water discharge from the drainage cannula and the removal of surface impurities, preventing foam or other floating debris from affecting water turbidity, and enhancing light penetration into the water.

[0025] According to one embodiment of the present invention, the spawning frame includes a fixed support and at least one spawning basket, a plurality of spawning meshes are arranged on the surface of the spawning basket, and a hook is provided on the spawning basket for fixing the spawning basket to the lower part of the fixed support;

[0026] A plurality of egg-laying baskets are arranged in parallel at the lower part of the fixing bracket along the length direction of the fixing bracket.

[0027] This design uses a fixed bracket to sink the spawning basket to the bottom and keep it stationary, creating a stable spawning environment. Multiple spawning meshes are arranged on the spawning basket to facilitate the fertilized eggs to wrap around the meshes. Hooks are provided on the spawning baskets to facilitate their removal. Once a spawning basket has accumulated a certain number of fertilized eggs, the fixed bracket can be lifted out of the water and replaced with a new one for the tiger squid to lay their eggs. Compared to conventional spawning baskets that float above the water, this design reduces the energy consumed by the squid when they swim to the surface to lay eggs. Furthermore, by placing multiple spawning baskets parallel to each other along the length of the fixed bracket, a single spawning frame placement allows for the collection of more fertilized eggs.

[0028] According to one embodiment of the present invention, floating rings are sleeved on the upper and lower sides of the spawning basket, and a spring is provided between two adjacent floating rings.

[0029] Through the above design, floating rings are set on the upper and lower sides of the spawning basket, which helps to reduce the interference of adjacent spawning baskets. It can also achieve a distance between the bottom of the spawning basket on the bottom side and the bottom of the breeding pond, so as to prevent dirt from getting too close to the spawning basket, affecting the eggs and causing the eggs to fall into the dirt. It can also solve the problem of the spawning basket tilting due to the slope at the bottom of the breeding pond, that is, the floating ring is used to correct the horizontality of the spawning basket in the water to avoid its tilt and getting stuck with the fixed bracket, making it difficult to remove; in addition, a spring is provided between two adjacent floating rings, and a certain flow peak gap can be formed by the spring between the two floating rings, which helps to displace dirt at the bottom of the breeding pond, especially when discharging sewage, and also ensures the water circulation at the bottom of the spawning basket to avoid hypoxia of the eggs at the bottom of the spawning basket.

[0030] A method for cultivating, maturing, and inducing spawning of tiger-striped squid in an indoor cement pool, using a device for cultivating, maturing, and inducing spawning of tiger-striped squid in an indoor cement pool, comprising the following steps:

[0031] S1: Build a breeding pond and disinfect it;

[0032] S2: seedling placement;

[0033] S3: Implement water quality management in aquaculture ponds;

[0034] S4: Regular feeding of bait and pond emptying and grading;

[0035] S5: Promote the maturation of broodstock and induce spawning.

[0036] Firstly, a culture pond body of a device for artificially cultivating tiger-striped squid in an indoor cement pond for breeding, accelerating maturation and inducing spawning is constructed, and the culture pond body is disinfected.

[0037] In the seeding step, the tiger squid seed used for parent culture is hatched and cultured from wild fertilized eggs, and the size of the selected body weight of about 400-500g is used as the initial seeding

[0038] The steps for implementing water quality management in the breeding pond are divided into three parts, namely water temperature control, salinity guarantee, and sewage suction and water exchange.

[0039] During the regular feeding phase, the bait is primarily fresh, unspoiled shrimp caught by Zhoushan's coastal trawlers. During the pond emptying and grading phase, the ponds are regularly cleaned to prevent outbreaks of Vibrio bacteria.

[0040] During the steps of ripening and inducing spawning of the parents, rapid spawning is induced by temperature control and placing spawning frames in the water body, and concentrated group reproduction is achieved through temperature control. BRIEF DESCRIPTION OF THE DRAWINGS

[0041] Figure 1 It is a three-dimensional schematic diagram of the breeding pond;

[0042] Figure 2 This is a top view of the breeding pond;

[0043] Figure 3 This is a schematic cross-sectional view of the aquaculture pond;

[0044] Figure 4 This is a schematic top view of a culture pond according to another embodiment;

[0045] Figure 5 This is a schematic cross-sectional view of a culture pond according to another embodiment;

[0046] Figure 6 Schematic diagram of the combination of the arc plate and the guard;

[0047] Figure 7 It is a three-dimensional schematic diagram of the curved plate;

[0048] Figure 8 This is a three-dimensional schematic diagram of the egg-laying frame.

[0049] Figure numbers: breeding pond body 1, water inlet pipe 11, drain outlet 12, first drain pipe 13, barrier cover 14, barrier mesh 141, second shaft 142, sleeve 15, arc plate 16, step 161, annular bar 162, first shaft 163, connecting rod 164, guard 17, guard plate 171, guard bar 172, guard rod 173, spawning frame 2, fixed bracket 21, spawning basket 22, hook 23, floating ring 24, spring 25, drainage cannula 31, drainage hole 32, second drain pipe 33. DETAILED DESCRIPTION

[0050] The technical solution of the present invention is further described in detail below with reference to the specific embodiments and the accompanying drawings:

[0051] Example 1:

[0052] like Figure 1 、 2 As shown in Figure 3, a device for artificially cultivating broodstock, promoting maturation and inducing spawning of tiger-striped squid in an indoor cement pool comprises a breeding pool body 1 and a plurality of spawning frames 2. A plurality of water inlet pipes 11 are provided in the breeding pool body 1, and a drain port 12 is provided on the bottom side of the breeding pool body 1. The drain port 12 is connected to a first drain pipe 13.

[0053] A barrier cover 14 is provided corresponding to the drain outlet 12 , and barrier meshes 141 are evenly distributed on the surface of the barrier cover 14 . A plurality of spawning frames 2 are used to be provided in the breeding pond body 1 .

[0054] Through the overall design of a device for artificially breeding, cultivating, maturing and inducing spawning of tiger-striped squid in an indoor cement pool, the breeding pool is a rectangular pool with rounded corners, 7.0m long, 4.0m wide and 1.5m high. A plurality of water inlet pipes 11 are arranged in the breeding pool body 1 to provide clean water rich in oxygen to the breeding pool body 1. A drain outlet 12 is provided at the bottom of the breeding pool body 1, and the drain outlet 12 is connected to a first drain pipe 13. The end of the first drain pipe 13 is lower than the surface of the water in the breeding pool body 1. The dirt, leftover bait, feces and other debris that have settled to the bottom of the breeding pool body 1 can be discharged from the drain outlet 12 through the drain outlet 12, thereby achieving cleaning of the breeding pool body 1. Improve the cleanliness of the water in the breeding pond 1 to prevent eutrophication of the water in the breeding pond 1 or the breeding of a large number of bacteria that may lead to the death of the tiger-striped squid; a barrier cover 14 is correspondingly provided at the drain outlet 12, the diameter of the barrier cover 14 is greater than 50 cm and the height is greater than 25 cm, and barrier meshes 141 are evenly distributed on the surface of the barrier cover 14. The size of the barrier mesh 141 can be flexibly adjusted according to the size and age of the tiger-striped squid. Through the setting of the barrier cover 14, it is possible to prevent the squid from being adsorbed on the plastic basket and being abraded and killed due to excessive drainage suction. The size of the barrier mesh 141 is adjusted to ensure that debris such as residual bait and feces at the bottom of the pond are discharged smoothly, while preventing juvenile squid from escaping therefrom.

[0055] By setting up the spawning frame 2, when most of the tiger-striped squids mate, the spawning frame 2 can be placed in the water body to induce them to spawn quickly. Since fertilized eggs have a positive tendency towards the spawning behavior of the tiger-striped squid, as long as a small number of fertilized eggs are attached, a large number of paired tiger-striped squids will then lay eggs on the spawning frame 2.

[0056] The bottom of the aquaculture pond 1 is tilted toward the drain 12. This design allows residual bait, feces, and other debris on the bottom of the aquaculture pond 1 to gather toward the drain 12, thereby improving the efficiency of the drain 12 in discharging waste, residual bait, feces, and other debris.

[0057] Furthermore, the entire bottom of the culture pond body 1 is inclined at an angle of 2 to 5 degrees toward the middle drain outlet 12. Furthermore, the entire bottom of the culture pond body 1 is inclined at an angle of 2 to 4 degrees toward the middle drain outlet 12.

[0058] The first drain pipe 13 is provided with a four-section sleeve 15 at the end thereof. This design allows the sleeve 15 to be pulled out step by step during drainage, facilitating the discharge of debris and preventing the squid from being attached to the plastic basket due to a large pressure difference between the inside and outside, which could result in injury or death of the squid.

[0059] Furthermore, the end portion of the sleeve 15 after being unfolded is higher than the surface of the water in the culture pond 1. Through the above design, the sleeve 15 can be unfolded when the culture pond 1 does not need to be drained, that is, no drainage is achieved.

[0060] The drainage assembly is also included. The drainage assembly includes a drainage cannula 31 vertically arranged in the culture body. A plurality of drainage holes 32 are provided on the upper portion of the drainage cannula 31 . The lower end of the drainage cannula 31 is connected to a second drain pipe 33 .

[0061] Through the above design, the drainage assembly is used to achieve a water flow function, that is, the drainage of the water in the aquaculture tank 1 is implemented through the multiple drainage holes 32 provided on the upper portion of the drainage cannula 31. Specifically, the surface or upper water is discharged through the drainage holes 32 into the second drainage pipe 33. The end of the second drainage pipe 33 is lower than the surface of the water in the aquaculture tank 1 and is discharged through the second drainage pipe 33. Since the drainage holes 32 are provided on the upper portion of the drainage cannula 31, air can be taken in at the top of the drainage pipe, thereby improving the water intake efficiency of the drainage holes 32 and facilitating the rapid discharge of dirt on the water surface with the running water.

[0062] Furthermore, a first pump body is provided within the aquaculture tank 1. The first pump body is located on the upper surface or upper layer of the water in the aquaculture tank 1 and is used to guide the surface water in the aquaculture tank 1 toward the drainage cannula 31. This design allows the surface water to flow toward the drainage cannula 31, improving the efficiency of water discharge from the drainage cannula 31 and the efficiency of removing surface impurities from the water. This prevents foam or other floating debris from affecting the turbidity of the water and improves the penetration of light into the water.

[0063] Furthermore, a first pump body is provided in the aquaculture tank 1, and the first pump body is used to guide the water in the aquaculture tank 1 to flow toward the drainage cannula 31. Through the above design, the outflow of clean water can be reduced, the waste of resources can be reduced, and the efficiency of the drainage component in discharging dirt on the water surface can be further improved.

[0064] The spawning frame 2 includes a fixed bracket 21 and an spawning basket 22 . A plurality of spawning meshes are arranged on the surface of the spawning basket 22 . A hook 23 is provided on the spawning basket 22 for fixing the spawning basket 22 to the lower part of the fixed bracket 21 .

[0065] The above design allows the fixed bracket 21 to sink the spawning basket 22 to the bottom and keep it stationary, creating a stable spawning environment. The spawning basket 22 is equipped with multiple spawning meshes, preferably with a mesh size of 1cm-1.5cm, to facilitate the fertilized eggs to wrap around the meshes. A hook 23 is provided on the spawning basket 22 to facilitate its removal. Once a spawning basket 22 has reached a certain number of fertilized eggs, the fixed bracket 21 can be lifted out of the water and replaced with a new spawning basket 22 for the tiger-striped cuttlefish to lay their eggs. Compared to conventional spawning baskets 22 that float on the water, this design reduces the energy consumed by the cuttlefish when swimming to the surface to lay eggs.

[0066] A method for cultivating, maturing, and inducing spawning of tiger-striped squid in an indoor cement pool, using a device for cultivating, maturing, and inducing spawning of tiger-striped squid in an indoor cement pool, comprising the following steps:

[0067] S1: constructing a culture pond 1 and disinfecting the culture pond 1;

[0068] S2: seedling placement;

[0069] S3: Implement water quality management in the aquaculture pond 1;

[0070] S4: Regular feeding of bait and pond emptying and grading;

[0071] S5: Promote the maturation of broodstock and induce spawning.

[0072] First, the aquaculture tank 1 described in the apparatus for artificially cultivating tiger-striped cuttlefish in an indoor cement tank for breeding, maturation, and induced spawning is constructed and disinfected. A sodium hypochlorite disinfectant solution is poured along the upper edge of the inner wall of the tank, flowing throughout the tank. Then, 10-15 cm of seawater is added, maintaining a concentration of at least 300-400 ppm to kill all potentially harmful bacteria, parasites, and other organisms. Tools used in the aquaculture process, such as drainage baskets, cannulas, bait baskets, scoops, scoop nets, air stones, and water inlet nets, are immersed in the highly concentrated sodium hypochlorite solution for 6-12 hours. The entire tank is thoroughly cleaned and then flushed with clean seawater before use.

[0073] In addition to disinfecting aquaculture tools (such as bait baskets, scoops, and scoop nets) by soaking them in a 300-400ppm sodium hypochlorite solution when cleaning a new pond (or emptying a pond), disinfect them every 3-5 days by placing them in a 150L red bucket (fresh water + 300-400ppm sodium hypochlorite solution). Rinse thoroughly with fresh water and air dry until ready for use. Rinse thoroughly with fresh water after each use and air dry until ready for use.

[0074] During the seed stocking process, tiger squid seed used for broodstock cultivation are hatched and cultured from wild fertilized eggs. Initial seed stocking is selected, weighing approximately 400-500g. As benthic cephalopods, tiger squid primarily rest on the pond bottom and swim within a small area, unlike migratory fish. This unique habit prevents them from being stocked at the same high densities as fish. Stocking density is determined by individual size, water quality, and the size of the pond floor. Generally, under good water quality (flow-through culture), the controlled density for tiger squid weighing 400-500g is 15-20 per square meter; for those weighing 750-1000g, the controlled density is 10-15 per square meter; and for those weighing 1500-2000g, the controlled density is 5-10 per square meter. Because tiger-striped squid mainly moves in the middle and lower layers of the water body, and the water quality conditions are guaranteed by the use of flow-through farming, the height of the water body in the indoor cement pool for tiger-striped squid farming does not need to be too high, generally 1.0-1.2m is sufficient.

[0075] The water quality management process in the culture pond 1 is divided into three parts, namely, water temperature control, salinity assurance, and sewage suction and water exchange. The water temperature control is to ensure that the tiger squid broodstock need to be heated during the winter. Generally, the water temperature is maintained at around 20-21 degrees during the winter. This temperature has three advantages: First, it ensures normal growth and feeding of tiger squid. If the temperature is too low, feeding will decrease, which is not conducive to growth. Second, it saves heating costs. Due to the low natural water temperature in winter, the water temperature in the aquaculture pond is generally maintained by oil boilers and heating rods (that is, the oil boiler raises the water temperature in the reservoir. During daily water changes, a water pump pumps the high-temperature water from the reservoir into the aquaculture pond and mixes it with the naturally low-temperature sand-filtered water to the required aquaculture water temperature. The heating rods are used to maintain a constant water temperature during the aquaculture pond flow). Excessively high temperatures will inevitably lead to high heating costs and waste resources. Third, it avoids precocious puberty. If the water temperature is too high for a long time, it can accelerate the arrival of the breeding period. After reaching the breeding period, the squid's growth rate slows and they will die after laying eggs. Relatively small squid not only produce fewer eggs but also have a low hatch rate of fertilized eggs, which is not conducive to reproduction. Our goal is to prioritize the growth of squid at the appropriate water temperature. Once they reach a larger size, we will use certain methods to induce spawning to achieve the goal of increasing egg production and fertilized egg quality.

[0076] Salinity is a key factor in the survival of tiger squid, as they require a relatively high salinity level, generally no less than 19‰. Winter rainfall is relatively low, and the salinity of natural seawater is relatively stable, which normally meets the tiger squid's salinity requirements. However, sufficient sea salt should still be available to prevent an unexpected drop in salinity that could cause acute damage to the squid.

[0077] Among them, sewage suction and water change are because squids often spray ink when competing for food or being stimulated. Excessive concentration of ink can also be toxic to the squid itself. Therefore, after cleaning the sewage in the morning and afternoon, water must be changed to ensure fresh water quality. The water change volume is about 40%-60% each time. After the water change, it is switched to micro-flow aquaculture. The flow rate is generally 15-20L / min. The flow rate should be controlled in combination with the number of heating rods. Generally, 30m3 of water body needs to be equipped with 3 2000w heating rods at this flow rate to ensure that the water cooling brought by the flow water and the water heating brought by the heating rods are balanced. The total daily water change volume is maintained at about 150%-200%.

[0078] During the regular feeding process, the bait is mainly composed of economic shrimps caught by Zhoushan's near-shore trawlers, such as the long-arm shrimp Ge's, brown shrimp or whiteleg shrimp. The size should be above 6-8 cm. If it is too small, it will be unpalatable. As the squid grows bigger, the size of the bait shrimp should also increase accordingly. Bait shrimp must be fresh and not deteriorate. It should be soaked in fresh water for 5 minutes when feeding. 2%-4% vitamin C, 1%-2% vitamin E and 6%-8% immune polysaccharides can be mixed in during soaking to enhance the resistance of squid. Feed at fixed points and times, adhere to the principle of small amounts and frequent meals, and feed at four time points a day: 06:00, 10:00, 14:00 and 18:00. The daily feeding amount is about 3%-8% of the squid's body weight (the proportion of daily feeding amount gradually decreases as the body weight increases). The feeding amount at 06:00 in the morning accounts for 30% of the daily feeding amount, the feeding amount at 10:00 and 14:00 accounts for about 15% of the daily feeding amount, and the feeding amount at 18:00 in the evening accounts for about 40% of the daily feeding amount. At the same time, the feeding amount should also be flexibly adjusted according to the specific feeding amount of the day and the leftover bait situation of the next day, so as to achieve sufficient feeding without wasting bait.

[0079] During the pond emptying and grading process, Vibrio spp. are a common bacterial group in the marine environment. They are highly adaptable and resistant to stress. After long periods of cultivation in the same pond, Vibrio spp. often cause disease outbreaks as pathogens. Therefore, regular pond emptying and cleaning are necessary to prevent disease. During the cultivation process, tiger squid grow at varying rates due to environmental stress or uneven food intake, and size variations between individuals are normal. Over time, these size variations become increasingly pronounced, impacting the reproductive capacity of the squid population. Therefore, pond emptying and grading are performed approximately every month, with small individuals separated and reared in separate ponds. During each emptying, the smallest 10%-15% of individuals are selected and transferred to separate ponds for separate rearing. A "last-in-first-out" competitive mechanism is employed, removing slow-growing and smaller individuals during emptying. This ensures that relatively uniform individuals maintain a high level of competition for food when offered, ensuring rapid overall growth, uniform size, and increased weight gain. Small squid raised individually also grow faster, gradually narrowing the size gap with larger squid. Tiger squid require different handling procedures than most other fish when restocking. While most fish can be scooped directly out of the water with a scoop net for a short period, their vitality is minimally affected upon restocking. However, squid, due to their ink-spraying nature, often experience ink-spraying when restocked using a standard scoop net. While ink-spraying doesn't directly kill the squid, it does have two drawbacks: first, ink-spraying depletes their own energy, impacting their vitality; and second, ink itself is toxic. If large amounts of ink are dissolved into a new pond and reach a certain concentration, it can also become toxic to the squid itself. To mitigate the effects of ink-spraying, squid must be transported with water when restocking. A special scoop net can be used. The lower part of the net is impermeable (no mesh) and the upper part is permeable (with mesh). This ensures that the squid is always in the water when it is scooped and transported, which can reduce the occurrence of ink spraying and avoid adverse situations such as damage or death caused by transportation.

[0080] During the broodstock maturation and spawning induction steps, rapid spawning is induced through temperature control and the placement of spawning frames (2) in the water. Temperature control also enables concentrated swarm breeding. During aquaculture, it was discovered that when tiger squid exhibit reproductive behaviors such as male and female chasing each other or mating, water temperature can be used to accelerate maturation and regulate the rate of spawning. When the water temperature is kept below 20°C, mating and spawning behavior is significantly reduced; however, when the water temperature is raised above 23°C, mating and spawning behavior is significantly increased. This characteristic allows us to lower the temperature when individual squid mate and slow down their reproductive behavior. Once the majority of squid mate, we can increase the temperature to accelerate swarm breeding. The advantages of this system are: first, it allows the cuttlefish to prioritize most of their energy for growth; larger individuals have greater reproductive capacity; second, it allows for the concentrated production of large numbers of fertilized cuttlefish eggs within a short period of time, making it easier for researchers to cultivate and grow their seedlings. A sparse production of fertilized eggs is highly unfavorable for later management, such as feeding, and the size differences between the cuttlefish can also lead to cannibalism. Spawning frames 2 can also be placed in the water to induce rapid spawning, as fertilized eggs have a positive tropism for the cuttlefish's spawning behavior. Once a small number of fertilized eggs are attached, a large number of paired cuttlefish will subsequently spawn on the spawning frames 2.

[0081] Using the aforementioned device and method for artificially cultivating, maturing, and inducing spawning of tiger-striped cuttlefish in an indoor cement tank, experiments began in early September 2022 with artificially cultivating broodstock of approximately 450g (hatched from fertilized eggs laid by wild cuttlefish). By mid-January 2023, the broodstock had reached approximately 1500g and had initially observed mating and spawning. Spawning frames 2 were then introduced, and the temperature was raised to approximately 23°C, prompting the squid to begin spawning in large numbers. After removing the spawning frames 2 and lowering the water temperature to 20°C, the spawning rate slowed. After a few days, the spawning frames 2 were reintroduced and the temperature raised, leading to another wave of spawning.

[0082] Fertilization and hatching rates of a subset of fertilized eggs from the initial spawning period were analyzed, along with experiments on the rearing of the next-generation juveniles. The results showed a fertilization rate of 59.05% and a hatching rate of 37.3%. By the end of February, over 700 first-generation juveniles with a carcass length of 2.0 cm had been produced. While the fertilization and hatching rates of fertilized eggs from captive-reared broodstock are not as high as those from wild eggs, the implementation of this technique has achieved a significant improvement over the previous situation where the hatching rate of fertilized eggs from captive-reared broodstock was almost zero, dispelling the common belief that captive-reared broodstock had a low overwintering survival rate and were unable to reproduce.

[0083] Example 2:

[0084] like Figure 4 、 5As shown in , 6 and 7, a device for artificially cultivating, ripening and inducing spawning of tiger-striped squid in an indoor cement pool according to another embodiment of the present invention is different from Example 1 in that a second shaft 142 is vertically provided on the top of the barrier cover 14, and an arc-shaped plate 16 is provided on the top of the second shaft 142. The arc-shaped plate 16 is bowl-shaped, and the concave side of the arc-shaped plate 16 faces the bottom side of the breeding pool body 1.

[0085] Through the above design, the second shaft 142 can form a certain distance between the arc plate 16 and the barrier cover 14. When the drain outlet 12 in the barrier cover 14 discharges sewage from the water in the breeding pond 1, it can limit the flow of water around the barrier cover 14 in the direction of the barrier cover 14, so that the water flowing into the drain outlet 12 flows along the bottom surface of the breeding pond 1 as much as possible, and can concentrate and effectively collect the dirt deposited on the bottom surface of the breeding pond 1, avoiding the formation of water flows gathering in all directions towards the barrier cover 14 in the breeding pond 1, and avoiding the water flow from concentrating a large amount of water in the breeding pond 1 toward the drain outlet 12 during sewage discharge, thereby preventing the tiger-striped squid from gathering toward the center of the breeding pond 1 under the guidance of the water, which may cause a stress response.

[0086] Furthermore, the concave side of the curved plate 16 is concentrically provided with a plurality of steps 161, and the ends of the plurality of steps 161 are provided with annular strips 162. The plurality of concentrically arranged steps 161 and annular strips 162 can slow down the water as it flows through, that is, reduce the flow rate of the water around the barrier cover 14 below the curved plate 16, and prevent the excessive flow rate from causing the tiger squid to gather in the center of the culture pond 1 under the guidance of the water, thereby reducing the probability of abrasions or stress reactions.

[0087] A first shaft 163 is provided on the top of the arc-shaped plate 16. The first shaft 163 is provided corresponding to the second shaft 142. A connecting rod 164 is provided on the top of the first shaft 163 symmetrically extending to both sides. The axis of the connecting rod 164 is provided parallel to the top surface of the barrier cover 14.

[0088] There is at least one guard member 17 on both sides of the connecting rod 164, and the guard member 17 includes a guard plate 171. The guard plate 171 is surrounded by a guard strip 172 on one side facing the bottom surface of the culture pond body 1, and a guard rod 173 is provided on the other side of the guard plate 171. The guard rod 173 is connected to the connecting rod 164.

[0089] Through the above design, the guard strips 172 provided on the guard plate 171 can protect the organisms and eggs, preventing them from moving toward the drain outlet 12 during sewage discharge, thereby protecting the organisms and eggs. The guard strips 172 allow debris such as dirt, leftover bait, and feces to move toward the drain outlet 12 without affecting the sewage discharge effect. At the same time, the guard plate 171 reduces the flow rate of the water around the spawning basket 22, thereby reducing the risk of eggs attached to the spawning basket 22 falling due to the influence of the water flow. The first shaft 163 can be configured to rotate or be fixed.

[0090] Example 3:

[0091] like Figure 8 As shown, according to another embodiment of the present invention, a device for artificially cultivating, maturing and inducing spawning of tiger-striped squid in an indoor cement pool is different from Example 1 in that floating rings 24 are provided on the upper and lower sides of the spawning basket 22, and a spring 25 is provided between two adjacent floating rings 24.

[0092] Through the above design, floating rings 24 are provided on the upper and lower sides of the spawning basket 22, which helps to reduce the interference of adjacent spawning baskets 22. It can also achieve a distance between the bottom of the spawning basket 22 on the bottom side and the bottom of the breeding pond 1, so as to prevent dirt from getting too close to the spawning basket 22, affecting the eggs and the eggs falling into the dirt. It can also solve the problem of the spawning basket 22 tilting due to the slope at the bottom of the breeding pond 1, that is, the floating ring 24 is used to correct the horizontality of the spawning basket 22 in the water to avoid its tilt and getting stuck with the fixed bracket 21, making it difficult to remove; in addition, a spring 25 is provided between two adjacent floating rings 24, and a certain flow peak gap can be formed by the spring 25 between the two floating rings 24, which helps to displace dirt at the bottom of the breeding pond, especially when discharging sewage, and also ensures the water circulation at the bottom of the spawning basket 22, avoiding hypoxia of the eggs at the bottom of the spawning basket 22.

[0093] The embodiments described above provide a detailed description of the technical solutions of the present invention. It should be understood that the above are only specific embodiments of the present invention and are not intended to limit the present invention. Any modifications, supplements or similar substitutions made within the scope of the principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A device for artificially cultivating broodstock, promoting maturation and inducing spawning of tiger-striped squid in an indoor cement pool, comprising a culture pool body (1) and a plurality of spawning frames (2), wherein a plurality of water inlet pipes (11) are provided in the culture pool body (1), a drainage port (12) is provided on the bottom side of the culture pool body (1), and the drainage port (12) is connected to a first drainage pipe (13); It is characterized by: A barrier cover (14) is provided corresponding to the drain outlet (12), and barrier meshes (141) are evenly distributed on the surface of the barrier cover (14). A plurality of spawning frames (2) are provided in the culture pond (1); A second shaft (142) is vertically provided on the top of the barrier cover (14), and a curved plate (16) is provided on the top of the second shaft (142). The curved plate (16) is bowl-shaped, and the concave side of the curved plate (16) faces the bottom side of the culture pond (1); A plurality of step portions (161) are concentrically provided on the concave side of the arc-shaped plate (16), and annular strips (162) are provided at the ends of the plurality of step portions (161); A first shaft (163) is provided on the top of the arc-shaped plate (16), and the first shaft (163) is arranged corresponding to the second shaft (142). A connecting rod (164) is symmetrically extended to both sides of the top of the first shaft (163), and the axis of the connecting rod (164) is arranged parallel to the top surface of the barrier cover (14); At least one guard member (17) is provided on both sides of the connecting rod (164), and the guard member (17) includes a guard plate (171). A guard strip (172) is arranged around one side of the guard plate (171) facing the bottom surface of the culture pond (1). A guard rod (173) is provided on the other side of the guard plate (171), and the guard rod (173) is connected to the connecting rod (164).

2. The device for artificially cultivating broodstock, accelerating maturation and inducing spawning of tiger-striped squid in an indoor cement pool according to claim 1, characterized in that: The inner bottom side of the culture pond body (1) is arranged to be inclined toward the drain outlet (12).

3. The device for artificially cultivating broodstock, accelerating maturation and inducing spawning of tiger-striped cuttlefish in an indoor cement pool according to claim 1, characterized in that: The end of the first drainage pipe (13) accommodates a sleeve (15) having multiple sections.

4. The device for artificially cultivating broodstock, accelerating maturation and inducing spawning of tiger-striped squid in an indoor cement pool according to claim 1, characterized in that: The invention also includes a drainage component, which includes a drainage cannula (31) vertically arranged in the culture pond body (1), a plurality of drainage holes (32) are provided on the upper part of the drainage cannula (31), and a second drain pipe (33) is connected to the lower end of the drainage cannula (31).

5. The device for artificially cultivating broodstock, accelerating maturation and inducing spawning of tiger-striped cuttlefish in an indoor cement pool according to claim 1, characterized in that: The spawning frame (2) includes a fixed bracket (21) and at least one spawning basket (22), a plurality of spawning meshes are arranged on the surface of the spawning basket (22), and a hook (23) is provided on the spawning basket (22), and the hook (23) is used to fix the spawning basket (22) to the lower part of the fixed bracket (21); A plurality of egg-laying baskets (22) are arranged in parallel at the lower portion of the fixing bracket (21) along the length direction of the fixing bracket (21).

6. The device for artificially cultivating broodstock, accelerating maturation and inducing spawning of tiger-striped cuttlefish in an indoor cement pool according to claim 5, characterized in that: Floating rings (24) are sleeved on the upper and lower sides of the spawning basket (22), and a spring (25) is provided between two adjacent floating rings (24).

7. A method for cultivating, maturing and inducing spawning of tiger-striped squid in an indoor cement pool, characterized in that: The device for artificially cultivating broodstock, accelerating maturation and inducing spawning of tiger-striped squid in an indoor cement pool as described in any one of claims 1 to 6 comprises the following steps: S1: constructing a breeding pond (1) and disinfecting the breeding pond (1); S2: seedling placement; S3: Implement water quality management in the aquaculture pond (1); S4: Regular feeding of bait and pond emptying and grading; S5: Promote the maturation of broodstock and induce spawning.

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