Application of Arabidopsis COL4 gene in improving plant resistance to UV-B

By overexpressing the Arabidopsis COL4 gene in plants, the problem of insufficient resistance to UV-B radiation in plants was solved, the UV-B resistance of plants was improved, and the growth capacity of plants in high UV-B environments was enhanced.

CN116836990BActive Publication Date: 2026-07-07SOUTH CHINA AGRICULTURAL UNIVERSITY

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SOUTH CHINA AGRICULTURAL UNIVERSITY
Filing Date
2022-03-24
Publication Date
2026-07-07

AI Technical Summary

Technical Problem

In the current technology, plants have insufficient resistance to UV-B radiation, which affects plant growth and yield, and the role of the Arabidopsis COL4 gene in UV-B resistance has not been fully studied.

Method used

By constructing an overexpression vector for the Arabidopsis COL4 gene and transforming it into plants using the inflorescence infection method, homozygous lines with overexpression of the COL4 gene were obtained, thereby improving the UV-B resistance of the plants.

Benefits of technology

It significantly improved plant resistance to UV-B, increased anthocyanin accumulation, reduced root length variation, and helped plants grow and develop in high UV-B environments.

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Abstract

The application provides application of Arabidopsis COL4 gene in improving UV-B resistance of plants. The application obtains an overexpression vector of Arabidopsis COL4 gene through genetic engineering technology, and thus obtains an overexpression strain of Arabidopsis, and finally obtains a homozygous strain of Arabidopsis COL4 gene overexpression through screening; the homozygous strain of Arabidopsis COL4 gene overexpression is characterized in that anthocyanin accumulation is increased under UV-B irradiation, root growth is better than that of a wild type, and good UV-B resistance is achieved. The application confirms through experiments that the Arabidopsis COL4 gene has potential application value in improving UV-B resistance of plants, and lays a good theoretical and application foundation for cultivating an UV-B-resistant and high-yield crop variety by using the Arabidopsis COL4 gene.
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