A method for improving the sensitivity of liquid chromatography-mass spectrometry for detecting vitamin K1 in serum

By using isopropanol instead of ammonium fluoride as a redissolving agent and adding ammonium fluoride solution after the liquid chromatography column eluent, the problems of column damage and insufficient sensitivity in vitamin K1 detection were solved, achieving efficient and low-cost quantitative detection.

CN116973465BActive Publication Date: 2026-05-01INGEDX TECHNOLOGIES CO LTD
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Patent Information

Application Number
CN202310429749.7
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2023-04-20
Publication Date
2026-05-01
Estimated Expiration
2043-04-20

AI Technical Summary

Technical Problem

In existing technologies, it is difficult to accurately quantify vitamin K1 in serum, especially since the addition of ammonium fluoride damages the chromatographic column, increasing detection costs and affecting detection sensitivity.

Method used

Isopropanol was used instead of ammonium fluoride as a redissolving agent, and ammonium fluoride solution was added after the liquid chromatography column eluted. Mass spectrometry detection was performed using an ESI ionization source to improve the sensitivity and recovery rate of vitamin K1 and reduce the system pressure of the chromatographic column.

Benefits of technology

This improved the sensitivity and recovery rate of vitamin K1 mass spectrometry detection, extended the lifespan of the chromatographic column, reduced detection costs, and met the requirements of clinical testing.

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Abstract

The application provides a method for improving the sensitivity of liquid chromatography-mass spectrometry in detecting vitamin K1 in serum, which comprises the following steps: preparing a sample solution of vitamin K1 to be detected by using a liquid-liquid extraction method to obtain an initial sample solution; and redissolving the initial sample solution by using an aqueous solution containing at least isopropanol as a redissolving agent to obtain a sample solution to be detected; and adding ammonium fluoride after an LC-MS column to improve the mass spectrometry detection sensitivity of the vitamin K1 sample. By using the technical scheme of the application, isopropanol or isopropanol+methanol is used as a redissolving agent to replace methanol in the prior art as a redissolving agent, the mass spectrometry detection sensitivity of VK1 is improved, the recovery rate is improved, the detection method has good stability, and the clinical detection requirements can be met; meanwhile, the ammonium fluoride in the liquid chromatography can be completely replaced, the system pressure of the liquid chromatograph is reduced, and the service life of the chromatographic column is prolonged.
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