An indoor multiplication device and method of leek maggot

Abstract

The application discloses an indoor propagation device and method of leek maggot, and relates to the technical field of insect propagation. The application provides an indoor propagation device of leek maggot, which comprises a cover and a shell. The cover is arranged above the shell. A first air hole for air ventilation is arranged on the surface of the cover. A rearing dish for rearing leek maggots is arranged in the shell through a support frame. The application further discloses a propagation method of the indoor propagation device of leek maggot. The indoor propagation device and method of leek maggot are used, and the work of adding water, moisturizing, picking eggs and picking insects does not need to consume a large amount of manpower, so that the leek maggots can be continuously reared indoors without being affected by the external environment, and the leek maggot population is provided for subsequent experiments.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of insect propagation, in particular to an indoor propagation device and method for Bradysia odoriphaga Yang et Zhang. BACKGROUND

[0002] Bradysia odoriphaga Yang et Zhang, also known as Bradysia odoriphaga Yang et Zhang, belongs to the family of Bradysia odoriphaga Yang et Zhang and is the most common and difficult to control underground pest in leek production. The pest has small body, fast reproduction speed, serious overlapping generations, and is distributed in soil in aggregation, which is difficult to control. Generally, the yield of a field is reduced by 40-60%, and even absolute yield is lost in severe cases. In order to control Bradysia odoriphaga Yang et Zhang, farmers frequently or excessively use high-toxic or highly-toxic insecticides, which leads to excessive pesticide residues in leeks, causes great hidden danger to food safety, and seriously pollutes soil and water sources and destroys the ecological environment.

[0003] The artificial mass propagation technology of test insects is one of the important means of modern pest control research. Bradysia odoriphaga Yang et Zhang is used as a research and control object, and in order to cooperate with the researches on biological control, drug resistance determination, and radiation sterility of Bradysia odoriphaga Yang et Zhang, it is very important to artificially mass breed and propagate Bradysia odoriphaga Yang et Zhang for test. However, the existing feeding technology of Bradysia odoriphaga Yang et Zhang mainly includes artificial feed, onions, and leeks, which are easy to cause feed putrefaction or rot due to humidity problems, resulting in a large number of deaths of Bradysia odoriphaga Yang et Zhang or affecting the growth and development of Bradysia odoriphaga Yang et Zhang. This brings difficulties to the research between leeks and Bradysia odoriphaga Yang et Zhang, and therefore a indoor propagation device and method for Bradysia odoriphaga Yang et Zhang are provided. SUMMARY

[0004] The present application aims to provide an indoor propagation device and method for Bradysia odoriphaga Yang et Zhang, which can realize indoor uninterrupted feeding of Bradysia odoriphaga Yang et Zhang without being affected by external environment and provide Bradysia odoriphaga Yang et Zhang population for subsequent experiments.

[0005] To achieve the above-mentioned purpose, the present application provides an indoor propagation device for Bradysia odoriphaga Yang et Zhang, which comprises a cover and a shell, the cover is arranged above the shell, the surface of the cover is provided with a first air hole for ventilation, the inside of the shell is provided with a rearing dish for feeding Bradysia odoriphaga Yang et Zhang, and the rearing dish is arranged in the inside of the shell through a support frame.

[0006] Preferably, the surface of the support frame is provided with a plurality of placing holes, and the rearing dish is arranged in the placing hole.

[0007] Preferably, the rearing dish is arranged in a bowl type, and the lower surface of the rearing dish is provided with a second air hole for ventilation.

[0008] Preferably, the diameter of the lower surface of the rearing dish is smaller than the diameter of the placing hole, and the diameter of the upper surface of the rearing dish is larger than the diameter of the placing hole.

[0009] The above-mentioned method for propagating leek maggots in an indoor environment includes the following steps:

[0010] S1. Place a certain amount of clean water inside the outer casing, then place the support frame inside the outer casing and fix it in place;

[0011] S2. Place two layers of filter paper inside the insect rearing dish, and place four layers of kitchen paper towels in between the filter paper. Then moisten the dish with distilled water, place the insect rearing dish on a support frame, put 1-2g of clayey wet soil and 2-5 fresh leek stems and leaves 6cm long in the insect rearing dish, then introduce 8-12 pairs of leek maggot adults to lay eggs, and place the insect rearing dish in a suitable environment for cultivation.

[0012] S3. Leek maggot eggs hatch in a rearing dish. During the 1st-2nd instar larval stage, add 2-5 fresh leek stems and leaves (6cm long) every 4 days. During the 3rd-4th instar larvae, add them once every 2 days until the leek maggots emerge.

[0013] S4. If the experiment requires leek maggots of the same age, the adult leek maggots can be transferred to a new rearing dish 24 hours after laying eggs to continue laying eggs until all the leek maggots die.

[0014] Preferably, the rearing conditions for insect rearing dishes are a temperature of 23-27℃ and a humidity of 60-90%.

[0015] Preferably, the growing conditions for chives are a temperature of 24-26℃, a humidity of 45-55%, and a photoperiod L:D = 14:10. Then, a layer of black synthetic fiber cloth is used to cover the chives to create a low-light environment.

[0016] The advantages and positive effects of the indoor propagation device and method for leek maggots described in this invention are as follows:

[0017] 1. The process for raising leek maggots in this invention is simple and easy to operate.

[0018] 2. This invention can complete the indoor propagation of leek maggots, providing a sufficient source of insects for leek maggot research.

[0019] 3. This invention eliminates the need for extensive manual labor for tasks such as adding water, maintaining humidity, selecting eggs, and removing larvae. It enables uninterrupted indoor rearing of leek maggots, unaffected by external environmental factors, thus providing a stable leek maggot population for subsequent experiments.

[0020] 4. This invention adds a small amount of soil to simulate the external environment for egg-laying, which is conducive to the egg-laying of leek maggots and facilitates the counting of eggs during the experiment. Furthermore, by directly feeding them natural feed—leeks—the population size is guaranteed to grow. Compared with existing methods for raising leek maggots, the method of this invention has a higher survival rate, pupation rate, eclosion rate, and single female egg-laying rate, providing favorable conditions for the normal supply of leek maggots.

[0021] The technical solution of the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. Attached Figure Description

[0022] Figure 1 This is a schematic diagram of the overall structure of an embodiment of an indoor propagation device for leek maggots according to the present invention;

[0023] Figure 2 This is an exploded view of an embodiment of an indoor propagation device for leek maggots according to the present invention.

[0024] Figure Labels

[0025] 1. Cap; 2. Outer shell; 3. Support frame; 4. Insect rearing dish; 5. First vent; 6. Second vent; 7. Placement hole. Detailed Implementation

[0026] The technical solution of the present invention will be further described below with reference to the accompanying drawings and embodiments.

[0027] Unless otherwise defined, the technical or scientific terms used in this invention shall have the ordinary meaning understood by one of ordinary skill in the art to which this invention pertains. The terms "first," "second," and similar terms used in this invention do not indicate any order, quantity, or importance, but are merely used to distinguish different components. Terms such as "comprising" or "including" mean that the element or object preceding the word encompasses the elements or objects listed following the word and their equivalents, without excluding other elements or objects. Terms such as "connected" or "linked" are not limited to physical or mechanical connections, but can include electrical connections, whether direct or indirect. Terms such as "upper," "lower," "left," and "right" are used only to indicate relative positional relationships; when the absolute position of the described object changes, the relative positional relationship may also change accordingly.

[0028] Example 1

[0029] like Figure 1 and Figure 2 As shown, an indoor propagation device for leek maggots includes a cover 1 and an outer shell 2. The cover 1 is positioned above the outer shell 2, and its surface has a first ventilation hole 5 for air circulation. Inside the outer shell 2, a rearing dish 4 for raising leek maggots is disposed, and the dish 4 is mounted inside the outer shell 2 via a support frame 3. The support frame 3 has several placement holes 7 on its surface, and the dish 4 is placed within these holes 7. The dish 4 is bowl-shaped, and its lower surface has a second ventilation hole 6 for air circulation. The diameter of the lower surface of the dish 4 is smaller than the diameter of the placement holes 7, and the diameter of the upper surface of the dish 4 is larger than the diameter of the placement holes 7.

[0030] More specifically, the length, width, and height of the outer shell 2 are set to 40cm, 40cm, and 9cm, respectively; the support frame 3 is placed 3cm away from the bottom of the outer shell 2; the bottom diameter of the insect rearing dish 4 is set to 6.9cm, the top diameter is set to 10.9cm, and the diameter of the placement hole 7 is set to 7.5cm.

[0031] When in use, a gauze is placed on top of the insect rearing dish 4, and an inner cover is placed on top of the gauze, with a ventilation opening on the inner cover.

[0032] Since temperature affects the reproductive cycle of leek maggots, the following examples investigate the effect of different humidity levels on the propagation of leek maggots at 25℃. The temperature and humidity settings in the following examples are shown in Table 1:

[0033] Table 1

[0034] Temperature / °C Humidity / % Example 2 25 60 Example 3 25 70 Example 4 25 75 Example 5 25 80

[0035] Example 2

[0036] A method for propagating leek maggots using an indoor propagation device includes the following steps:

[0037] S1. Place 1.5cm of clean water inside the outer casing 2, then place the support frame 3 inside the outer casing 2 and fix it in place;

[0038] S2. Place two layers of filter paper inside the insect rearing dish 4, and place four layers of kitchen paper towels in between the filter paper. Then moisten the paper towels with distilled water. Place the insect rearing dish 4 on the support frame 3. Put 1g of sticky wet soil and two 6cm long fresh chive stems and leaves into the insect rearing dish 4. Then introduce 10 pairs of adult chive maggots to lay eggs. Place the insect rearing dish 4 in an environment with a temperature of 25℃ and a humidity of 60% for cultivation. The chives are grown at a temperature of 24-26℃ and a humidity of 45-55%, with a photoperiod L:D = 14:10. Then cover the dish with a layer of black synthetic fiber cloth to create a low-light environment.

[0039] S3. Leek maggot eggs are hatched in rearing dish 4. During the 1st and 2nd instar larval stage, 4 fresh leek stems and leaves, each 6cm long, are added every 4 days. During the 3rd and 4th instar larvae stage, they are added every 2 days until the leek maggots emerge. The survival rate, pupation rate, emergence rate, and number of eggs laid by a single female are recorded.

[0040] The results are shown in Table 2:

[0041] Table 2

[0042] Survival rate Pupation rate Emergence rate Egg production per female 75.31％ 89.75％ 90.17％ 67 grains

[0043] Example 3

[0044] A method for propagating leek maggots using an indoor propagation device includes the following steps:

[0045] S1. Place 1.5cm of clean water inside the outer casing 2, then place the support frame 3 inside the outer casing 2 and fix it in place;

[0046] S2. Place two layers of filter paper inside the insect rearing dish 4, and place four layers of kitchen paper towels in between the filter paper. Then moisten the paper towels with distilled water. Place the insect rearing dish 4 on a support frame. Put 1g of sticky wet soil and two 6cm long fresh chive stems and leaves into the insect rearing dish 4. Then introduce 10 pairs of adult chive maggots to lay eggs. Place the insect rearing dish 4 in an environment with a temperature of 25℃ and a humidity of 70% for cultivation. The chives are grown at a temperature of 24-26℃ and a humidity of 45-55%, with a photoperiod L:D = 14:10. Then cover the dish with a layer of black synthetic fiber cloth to create a low-light environment.

[0047] S3. Leek maggot eggs are hatched in a rearing dish. During the 1st and 2nd instar larval stage, 4 fresh leek stems and leaves, each 6cm long, are added every 4 days. During the 3rd and 4th instar larvae stage, they are added every 2 days until the leek maggots emerge. The survival rate, pupation rate, emergence rate, and number of eggs laid by a single female are recorded.

[0048] The results are shown in Table 3:

[0049] Table 3

[0050] Survival rate Pupation rate Emergence rate Egg production per female 80.95％ 91.17％ 93.35％ 102 grains

[0051] Example 4

[0052] A method for propagating leek maggots using an indoor propagation device includes the following steps:

[0053] S1. Place 1.5cm of clean water inside the outer casing 2, then place the support frame 3 inside the outer casing 2 and fix it in place;

[0054] S2. Place two layers of filter paper inside the insect rearing dish 4, and place four layers of kitchen paper towels in between the filter paper. Then moisten the paper towels with distilled water. Place the insect rearing dish 4 on a support frame. Put 1g of sticky wet soil and two 6cm long fresh chive stems and leaves into the insect rearing dish 4. Then introduce 10 pairs of adult chive maggots to lay eggs. Place the insect rearing dish 4 in an environment with a temperature of 25℃ and a humidity of 75% for cultivation. The chives are grown at a temperature of 24-26℃ and a humidity of 45-55%, with a photoperiod L:D = 14:10. Then cover the dish with a layer of black synthetic fiber cloth to create a low-light environment.

[0055] S3. Leek maggot eggs are hatched in rearing dish 4. During the 1st and 2nd instar larval stage, 4 fresh leek stems and leaves, each 6cm long, are added every 4 days. During the 3rd and 4th instar larvae stage, they are added every 2 days until the leek maggots emerge. The survival rate, pupation rate, emergence rate, and number of eggs laid by a single female are recorded.

[0056] The results are shown in Table 4:

[0057] Table 4

[0058] Survival rate Pupation rate Emergence rate Egg production per female 81.06％ 91.33％ 93.41％ 125 grains

[0059] Example 5

[0060] A method for propagating leek maggots using an indoor propagation device includes the following steps:

[0061] S1. Place 1.5cm of clean water inside the outer casing 2, then place the support frame 3 inside the outer casing 2 and fix it in place;

[0062] S2. Place two layers of filter paper inside the insect rearing dish 4, and place four layers of kitchen paper towels in between the filter paper. Then moisten the paper towels with distilled water. Place the insect rearing dish 4 on a support frame. Put 1g of sticky wet soil and two 6cm long fresh chive stems and leaves into the insect rearing dish 4. Then introduce 10 pairs of adult chive maggots to lay eggs. Place the insect rearing dish 4 in an environment with a temperature of 25℃ and a humidity of 80% for cultivation. The chives are grown at a temperature of 24-26℃ and a humidity of 45-55%, with a photoperiod L:D = 14:10. Then cover the dish with a layer of black synthetic fiber cloth to create a low-light environment.

[0063] S3. Leek maggot eggs are hatched in a rearing dish. During the 1st and 2nd instar larval stages, 5 fresh leek stems and leaves, each 6cm long, are added every 4 days. During the 3rd and 4th instar larvae stages, they are added once every 2 days until the leek maggots emerge. The survival rate, pupation rate, emergence rate, and number of eggs laid by a single female are recorded.

[0064] The results are shown in Table 5:

[0065] Table 5

[0066] Survival rate Pupation rate Emergence rate Egg production per female 85.71％ 95.83％ 93.48％ 142 grains

[0067] When cultivated in an environment with a temperature of 25℃ and a humidity of 90%, the chives were prone to rotting due to the high humidity. The egg production, survival rate, pupation rate, and emergence rate of chive maggots were all low and unstable, so no statistics were collected.

[0068] As shown in Tables 1, 2, and 3, under 25℃ conditions, the number of eggs laid by a single female leek maggot gradually increases with the increase of humidity. However, excessively high humidity (90%) will cause the leeks to rot easily, and reduce the survival rate, pupation rate, and emergence rate of leek maggots, which is not conducive to the propagation of leek maggots.

[0069] When cultured in an environment with a temperature of 25℃ and a humidity of 80%, the leek maggots exhibit the highest egg production per female, survival rate, pupation rate, and emergence rate, which is most conducive to their propagation. Therefore, the optimal conditions for the propagation of leek maggots are a temperature of 25℃ and a humidity of 80%.

[0070] The present invention has a simple breeding process, is easy to operate, and can raise leek maggots indoors without being affected by the external environment.

[0071] This invention simulates the external environment for egg-laying by adding a small amount of soil, which is beneficial for leek maggots to lay eggs and facilitates counting the number of eggs laid during experiments. Furthermore, by directly feeding them natural feed—leeks—the population size is guaranteed to grow. Compared with existing methods for raising leek maggots, the method of this invention has a higher survival rate, pupation rate, eclosion rate, and single female egg-laying rate, providing favorable conditions for the normal supply of leek maggots.

[0072] Therefore, the present invention adopts the above-mentioned indoor propagation device and method for leek maggots, which eliminates the need for a large amount of manual labor for adding water, keeping the soil moist, picking eggs and maggots, and can realize uninterrupted indoor breeding of leek maggots without being affected by the external environment, thus providing a stable leek maggot population for subsequent experiments.

[0073] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit them. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can still be made to the technical solutions of the present invention, and these modifications or equivalent substitutions cannot cause the modified technical solutions to deviate from the spirit and scope of the technical solutions of the present invention.

Claims

1. A method for propagating leek maggots using an indoor propagation device, characterized in that, The indoor propagation device for leek maggots includes a cap and an outer shell. The cap is located on top of the outer shell and has a first ventilation hole on its surface. Inside the outer shell is a rearing dish for raising leek maggots, which is set inside the outer shell by a support frame. The surface of the support frame is provided with several placement holes, and the insect rearing dish is placed in the placement holes; The insect rearing dish is bowl-shaped, and a second vent is provided on the lower surface of the insect rearing dish for ventilation. The diameter of the lower surface of the insect rearing dish is smaller than the diameter of the placement hole, and the diameter of the upper surface of the insect rearing dish is larger than the diameter of the placement hole. The propagation method includes the following steps: S1. Place a certain amount of clean water inside the outer casing, then place the support frame inside the outer casing and fix it in place; S2. Place two layers of filter paper inside the insect rearing dish, and place four layers of kitchen paper towels in between the filter paper. Then moisten the dish with distilled water, place the insect rearing dish on a support frame, put 1-2g of clayey wet soil and 2-5 fresh leek stems and leaves 6cm long in the insect rearing dish, then introduce 8-12 pairs of leek maggot adults to lay eggs, and place the insect rearing dish in a suitable environment for cultivation. The cultivation conditions are: temperature 25℃, humidity 80%; S3. Leek maggot eggs hatch in a rearing dish. During the 1st-2nd instar larval stage, add 2-5 fresh leek stems and leaves (6cm long) every 4 days. During the 3rd-4th instar larvae, add them once every 2 days until the leek maggots emerge. S4. If the experiment requires leek maggots of the same age, transfer the adult leek maggots to a new rearing dish 24 hours after they lay eggs to continue laying eggs until all the leek maggots die.

2. The propagation method of the indoor propagation device for leek maggots according to claim 1, characterized in that: The ideal growing conditions for chives are a temperature of 24-26℃, humidity of 45-55%, and a photoperiod of L:D = 14:

10. Then, cover the chives with a layer of pure black synthetic fiber cloth to create a low-light environment.

Images