A method for cutting propagation of super-tender shoots of Clitoria ternatea

Abstract

The application discloses a kind of super tender tip cuttage propagation methods of Clitoria ternatea, it is related to Clitoria ternatea propagation technical field.The application method uses the super tender tip part of Clitoria ternatea without lignification, its length is 1.5-2cm, is about 1 / 5 of existing tender branch cuttage cutting piece, and qualified cutting piece can be quickly sprouted on the mother plant of shortening, solve the situation that cuttage propagation cutting piece is not enough, can meet or improve market demand.Further, by using rooting accelerator, the rooting rate and survival rate of cuttage seedling are improved, and large-scale seedling raising can be realized.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of Robinia pseudoacacia L. propagation, in particular to a super-tender shoot cutting propagation method of Robinia pseudoacacia L. BACKGROUND

[0002] Robinia pseudoacacia L. is a deciduous shrub of the legume genus Robinia, which is a good ornamental, medicinal and forage plant in the north, has the characteristics of flowering in summer and autumn, excellent ornamental effect, strong shade tolerance, developed root system, and tolerance to poor soil, etc. It has been widely used as a representative plant of oriental characteristic landscape in European and American nurseries. At present, with the large application in ecological restoration, landscape application, medicine and forage, the supply of high-quality Robinia pseudoacacia L. seedlings is in short supply in the near future, which cannot meet the market demand.

[0003] Robinia pseudoacacia L. mainly has two propagation forms of seed propagation and cutting propagation. However, the seed coat of Robinia pseudoacacia L. is hard and cannot germinate normally, and needs to be pretreated to remove the dormancy caused by hard seed. The seedling has the defects of individual trait differentiation and difficulty in maintaining good seed characteristics, which cannot guarantee the quality of good seed wood supply. Cutting propagation has the advantages of convenient material taking, short seedling period, large propagation coefficient, fast growth, low cost and good maintenance of the excellent characteristics of the mother plant. However, due to the spring sprouting of Robinia pseudoacacia L., the middle and upper branches have low lignification degree and storage nutrient backflow, resulting in low survival rate of spring hardwood cutting. The length of the semi-lignified branch used as cutting stock in summer cutting is 8-10 cm, and the survival rate of cutting is improved, generally about 85%, but the required cutting length is long and affected by seasonality, and the amount of cutting from a single plant is small, which is difficult to meet the demand of large-scale seedling raising.

[0004] Therefore, it is an urgent problem for those skilled in the art to provide a Robinia pseudoacacia L. propagation method which can overcome the above shortcomings and meet the market demand. SUMMARY

[0005] Therefore, the present application provides a super-tender shoot cutting propagation method of Robinia pseudoacacia L. The method uses the super-tender shoot part of Robinia pseudoacacia L. which has no lignification, and the length is 1.5-2 cm, which is about 1 / 5 of the length of the existing tender shoot cutting. The mother plant can quickly grow qualified cutting stock after being cut, which solves the problem of insufficient cutting stock for cutting propagation and can meet or improve the market demand. Further, the use of rooting accelerators improves the rooting and survival rate of the cutting seedlings, and large-scale seedling raising can be realized.

[0006] In order to achieve the above purpose, the present application adopts the following technical scheme:

[0007] A rooting accelerator, the preparation method of the rooting accelerator is: the early spring bud of Syringa oblata is ground by liquid nitrogen, the powder is added into distilled water in a mass-volume ratio of 1:500, ultrasonic extraction is carried out for 30 min, after standing for 3-5 min, quantitative filter paper is used for filtering; the obtained filtrate is centrifuged at 6000 r / min for 5 min, and the supernatant is obtained.

[0008] As a preferred technical scheme, the early spring bud of Syringa oblata is first washed with distilled water, dried, and then ground by liquid nitrogen.

[0009] As a preferred technical scheme, the ultrasonic extraction is carried out at 25 DEG C.

[0010] Another object of the present application is to provide a super-nitrous tip cutting propagation method of Lespedeza bicolor, comprising the following steps:

[0011] (1) cutting obtaining and processing: in 6-8 months, the vigorous growth period, the tender shoots with 1-2 young leaves and non-lignified of 2-7 year-old mother plants are taken as cuttings, the length of the tender shoots is 1.5-2 cm, and the tender shoots have 1-2 internodes;

[0012] The upper end of the cutting is cut flat, the lower end is obliquely cut, and the lower end, i.e. the base, is quickly dipped in the rooting accelerator in any one of claims 1-3 for 5 s;

[0013] (2) cutting: the quickly dipped cutting is inserted into the cutting substrate with a depth of 1 cm; after cutting, water is poured in time, and a disinfecting solution is sprayed for disinfection;

[0014] (3) management after cutting: after cutting, shading treatment is carried out, the shading degree is 70%, the air humidity is controlled to be 93%-98%, and the substrate is disinfected regularly with the disinfecting solution.

[0015] As a preferred technical scheme, the super-nitrous tip cutting propagation method of Lespedeza bicolor further comprises bed preparation: a 54 cm x 28 cm hole tray is placed on a greenhouse cutting bed, and the cutting substrate is a mixture of grass carbon soil and perlite at a ratio of 1:1, which is disinfected with 0.3% carbendazim solution before use.

[0016] As a preferred technical scheme, the mother plant is 3-6 years old, preferably 4-5 years old, in step (1).

[0017] As a preferred technical scheme, the disinfecting solution in steps (2) and (3) is 0.3% carbendazim solution.

[0018] As a preferred technical scheme, the regular period in step (3) is every 7 days.

[0019] Beneficial effects:

[0020] 1. The rooting accelerator of the present application is a Syringa extract. The use of the accelerator for treating the cuttings before cutting can significantly improve the rooting rate and root growth of the cuttings.

[0021] 2. The cutting method of the present application uses non-lignified super-tender shoots as cuttings, which are 1.5-2 cm long, only about 1 / 5 of the length of the existing tender branch cuttings. The mother plant can quickly grow qualified cuttings after being cut, and the yield of the mother plant is about 6 times that of the existing tender branch cuttings. BRIEF DESCRIPTION OF DRAWINGS

[0022] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the drawings needed in the following embodiment or prior art description will be briefly introduced. Obviously, the drawings in the following description are only a part of the embodiments of the present application, and for those skilled in the art, other drawings can be obtained without creative labor based on the provided drawings.

[0023] Figure 1 Fig. 1 is a schematic diagram of the growth of different ages of Clitoria ternatea of the present application. DETAILED DESCRIPTION

[0024] The technical solutions in the embodiments of the present application will be described clearly and completely in the following with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor are within the scope of protection of the present application.

[0025] Embodiment 1

[0026] Super-tender shoot cutting yield statistics

[0027] In June-August 2022, different ages of 0-7 years old mother plants were selected for cutting yield comparison. 30 plants with consistent growth were randomly selected for each tree age in each group for statistics. The growth of the mother plant branches was observed every 7 days. The number of cuttings and the interval time for cutting were determined according to the required length of the cuttings.

[0028] Super-tender shoot cutting standard: 1-2 non-lignified branches with 1-2 leaflets, 1.5-2 cm long, with 1-2 internodes.

[0029] Tender branch cutting standard (traditional method for comparison): semi-lignified branches of the current year, 8-10 cm long; super-tender shoot cuttings are selected from non-lignified branches at the top of the external branches, 1.5-2 cm long.

[0030] The yield of single plant of different ages of Clitoria ternatea in two forms was observed and compared (see Table 1).

[0031] Table 1 Comparison of single plant cutting yield of Clitoria ternatea mother plants

[0032]

[0033] Note: The seedling age 0 represents the seedlings sown in spring of the current year.

[0034] From the statistical results in Table 1, the number of cuttings that can be collected from super-tender shoots and traditional tender shoots both show a "first increase and then decrease" trend with the increase of seedling age. Except for 0 and 1 years, a certain number of cuttings can be obtained, and the 4-5 year old mother plants can provide the most cuttings. The length of super-tender shoots is about 1 / 5 of the length of tender shoots. Under the same seedling age, the number of super-tender shoot cuttings is about 5 times more than that of tender shoot cuttings. This is related to the characteristics of Clitoria ternatea, such as tolerance to pruning, easy to sprout, and fast growth in summer. A large number of cuttings can be obtained in a short time, providing cutting materials for large-scale propagation.

[0035] Example 2

[0036] 1. Preparation of rooting promoter

[0037] The collected Syringa early spring sprouting buds were washed with distilled water for 3-5 times, the surface water of the buds was absorbed with filter paper, and the buds were ground in liquid nitrogen. 4g of powder was added to 2L of distilled water, stirred uniformly, and then ultrasonically extracted for 30min at 25℃. After standing for 3-5min, the filtrate was filtered with a 90mm diameter quantitative filter paper. The obtained filtrate was centrifuged at 6000r / min for 5min, and the supernatant was the Syringa extract stock solution.

[0038] The Syringa extract stock solution was diluted with distilled water to obtain 1:1000, 1:2000, and 1:4000 extracts, which were used as rooting promoters for Clitoria ternatea super-tender shoots.

[0039] 2. Cutting test

[0040] In early July 2023, greenhouse cutting propagation of Clitoria ternatea was carried out using the rooting promoter prepared in Example 2. The specific propagation method is as follows:

[0041] (0) Preparation of seedbed: Place 54cmx28cm hole trays on the greenhouse cutting seedbed. The cutting substrate is a mixture of grass charcoal soil and perlite at a ratio of 1:1, which is sterilized with 0.3% carbendazim solution before use.

[0042] (1) Select 4-5 year old Clitoria ternatea mother plants with healthy growth, and obtain tender shoots containing 1-2 pieces of non-lignified young leaves as asexual propagation materials. The length of the tender shoots is 1.5-2cm, and they have 1-2 internodes.

[0043] The length of the tender shoot cuttings is 8-10cm, which is used as a traditional cutting comparison group.

[0044] The upper end of the cutting is cut flat, and the lower end is cut obliquely.

[0045] (2) The base of the cutting is sterilized in 0.3% carbendazim solution for 3-5 minutes, then is treated by being quickly dipped in rooting accelerator for 5 seconds, and then is cut in a mixed substrate of grass charcoal soil and perlite (1:1) which is sterilized by 0.3% carbendazim.

[0046] Water treatment is used as a control, 30 cuttings are used for each treatment, and 3 repetitions are performed.

[0047] (3) Water is poured in time after cutting, and 0.3% carbendazim solution is sprayed once for sterilization, the air humidity is controlled to be 93%-98%, and the shading degree is 70%.

[0048] (4) 0.3% carbendazim solution is used for sterilization every 7 days after cutting.

[0049] (5) The rooting condition is investigated and counted 30 days after cutting (part of the cuttings gradually die after cutting for 7 days, part of the cuttings begin to produce callus at the base, the adventitious roots are formed and begin to grow new leaves after about 14 days, the dead cuttings do not grow new leaves and do not root. The cuttings that survive after 30 days have all entered the adventitious root elongation stage, and the plants that do not root have all died. Therefore, the rooting rate, the survival rate and the propagation coefficient are the same after 30 days of cutting of the present application, and the rooting rate is displayed).

[0050] Table 2: Influence of rooting accelerator with different mass-volume ratios on the rooting of Clitoria ternatea

[0051]

[0052]

[0053] It can be known from the statistical results in Table 2 that the rooting accelerator of the present application shows the trend of "first increasing and then decreasing" in the rooting rate, the rooting number and the root length index for both types of cuttings with the increase of the concentration of the rooting accelerator. After being treated by the rooting accelerator with a mass-volume ratio of 1:2000, the rooting effect of both types of cuttings is the best. Under the treatment of the same concentration, there is no obvious difference between the super-tender shoot cutting propagation and the tender branch cutting propagation in the rooting rate, the rooting number and the average root length. It is shown that the super-tender shoot cloning propagation and the tender branch cutting propagation method after being treated by the early spring bud extract of Syringa oblata can significantly improve the propagation efficiency.

[0054] The embodiments in the specification are described in a progressive manner, and each embodiment focuses on the difference from other embodiments. The same or similar parts of each embodiment can be referred to each other.

[0055] The foregoing description of the disclosed embodiments enables a person skilled in the art to make or use the application. Modifications of these embodiments will occur to persons of skill in the art, and that the appended claims are intended to cover all such modifications that do not depart from the true spirit and scope of the application. Therefore, the application is not limited to the embodiments shown but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A method for propagating Lespedeza bicolor by cuttings from very young shoots, characterized in that, Includes the following steps: (1) Cuttings acquisition and treatment: From June to August, during the vigorous growth period, take tender shoots with 1-2 young leaves from mother plants aged 2-7 years as cuttings. The tender shoots are 1.5-2 cm long and have 1-2 internodes. Cut the top of the cutting flat and the bottom at an angle. Dip the bottom part (base) into the rooting promoter for 5 seconds. (2) Cuttings: Insert the quick-dipped cuttings into the cutting substrate to a depth of 1 cm; water them promptly after cutting and spray with disinfectant solution for disinfection; (3) Post-cutting management: Shade treatment after cutting, with a shading degree of 70% and air humidity controlled at 93%-98%; disinfect the substrate regularly with disinfectant solution; The preparation method of the rooting promoter in step (1) is as follows: the early spring buds of lilac collected are rinsed with distilled water 3-5 times, the surface moisture of the buds is dried with filter paper, and the buds are ground thoroughly with liquid nitrogen. 4 g of powder is added to 2 L of distilled water, stirred evenly, and then extracted by ultrasonication at 25 ℃ for 30 min. After standing for 3-5 min, the mixture is filtered with quantitative filter paper with a diameter of 90 mm. The obtained filtrate is centrifuged at 6000 r / min for 5 min, and the supernatant is the original lilac extract. The original lilac extract is diluted with distilled water to obtain a 1:2000 extract, which is used as the rooting promoter.

2. The method for propagating Lespedeza multiflora by cuttings using ultra-tender shoots according to claim 1, characterized in that, It also includes seedbed preparation: 54 cm x 28 cm seedling trays are placed on the greenhouse cutting bed, and the cutting substrate is a 1:1 mixture of peat moss and perlite, which is disinfected with 0.3% carbendazim solution before use.

3. The method for propagating Lespedeza bicolor by cuttings using ultra-tender shoots according to claim 1, characterized in that, Step (1) The mother plant seedlings are 3-6 years old.

4. The method for propagating Lespedeza bicolor by cuttings using ultra-tender shoots according to claim 3, characterized in that, Step (1) The mother plant seedlings are 4-5 years old.

5. The method for propagating Lespedeza bicolor by cuttings using ultra-tender shoots according to claim 1, characterized in that, The disinfectant solution in steps (2) and (3) is a 0.3% carbendazim solution.

6. The method for propagating Lespedeza bicolor by ultra-tender shoot cuttings according to claim 1, characterized in that, The periodicity described in step (3) is every 7 days.

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