Anhydrous mouthwash and use thereof

Abstract

The application discloses anhydrous mouthwash and application thereof, and the mouthwash is prepared from coconut water, sodium hyaluronate, FE biological lysozyme and a preservative. The formula of the application is helpful to maintain the activity of the FE biological lysozyme and prolong the shelf life of the mouthwash. The mouthwash has certain effects on reducing dental plaque, gum bleeding and gum inflammation.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of oral care, in particular to a water-free mouthwash and application thereof. BACKGROUND

[0002] The oral cavity is the starting point of the respiratory system and the digestive system of the human body, and oral health is closely related to overall health, directly affecting people's daily life quality, and may also be the culprit of diseases in the heart, stomach, lungs and other parts of the body. Mouthwash is a common oral cleaning product, commonly used for cleaning the oral cavity, reducing bad breath, maintaining oral health, etc. In recent years, oral health has received increasing attention from the public, and the oral health awareness of Chinese residents has significantly improved. People have gradually discovered that up to 40% of the tooth surface cannot be cleaned with a toothbrush, and functional mouthwash with antibacterial and bacteriostatic effects has good penetration, can penetrate into various parts of the oral cavity, including the gum margin and interdental spaces that are difficult to access by a toothbrush, thereby playing an effective antibacterial and bacteriostatic role, preventing caries, gingivitis and bad breath caused by microorganisms, and improving oral health. At present, the bacteriostatic and bactericidal components in mouthwash on the market are mostly chemical synthetic components, which have certain toxic side effects and irritation to the oral mucosa, and also have the possibility of causing allergies, reducing the experience comfort and acceptance of users. In particular, the problem of drug resistance caused by long-term use of antibacterial drugs has also attracted attention. Consumers have greater interest in the use of "natural" antibacterial agents.

[0003] A raw material composition of cassia twig mouthwash and its use are disclosed in Chinese granted invention patent CN114432201B. The raw material composition of cassia twig mouthwash includes the following raw materials mixed uniformly in volume percentage: cassia twig steam distillation liquid 80% to 100%, anhydrous ethanol 0 to 20%; wherein the preparation method of the cassia twig steam distillation liquid includes the following steps: weighing a proper amount of cassia twig, adding 15 to 20 times the amount of pure water, steam distilling for 5 to 6 hours, collecting the distillation liquid every 20 to 30 minutes, and the collection ratio is 50 to 100 ml per 100 g of cassia twig. The product contains specific proportions of coumarin, cinnamyl alcohol, cinnamic acid, cinnamaldehyde, o-methoxycinnamaldehyde, etc., and has better bacteriostatic and bactericidal effects than the marketed cassia twig mouthwash products. The raw material composition of the cassia twig mouthwash of the present application is simple to prepare, the raw materials are easy to obtain, the quality is stable, can quickly inhibit bacteria and kill bacteria, keep the oral cavity clean and free of bad breath, and has good taste, suitable for daily use.

[0004] In addition, in the prior invention patent CN111514161B of the present application, an anti-oral ulcer preparation is disclosed, which is characterized in that it comprises the following components in mass percentage: 80-90% of sea squirt polysaccharide 1, 2-20% of lactobionic acid, 0.05-1% of calcium salt, and 0.5-1% of plant preservative. The above-mentioned anti-oral ulcer preparation can be used in the preparation of film, ointment, powder, granule, toothpaste, mouthwash, oral spray and other products for treating oral ulcer. The invention uses marine polysaccharide sea squirt polysaccharide 1 as the main mucosal repair component, relieves inflammatory reaction, and promotes the healing of ulcer tissue and the repair of the surface of the ulcer; lactobionic acid and calcium salt play a buffering and synergistic role in the repair process; the natural plant-derived preservative is mild and safe, and has no irritation or allergic reaction.

[0005] FE biological lysozyme is an active biological protease that can simulate the function of salivary enzymes and kill harmful bacteria in the oral cavity. However, when FE biological lysozyme is applied to mouthwash, the problem of difficult persistence of its biological activity exists, resulting in a short shelf life of the product. The present invention is truly based on research and development on how to improve the stability of FE biological lysozyme in mouthwash, and is completed. SUMMARY

[0006] Based on the above background technology, the technical problem to be solved by the present invention is to provide anhydrous mouthwash and its application. In order to achieve the purpose of the present invention, the following technical solutions are adopted:

[0007] The present invention relates to an anhydrous mouthwash, which is prepared from coconut water, sodium hyaluronate, FE biological lysozyme and a preservative.

[0008] In a preferred embodiment of the present invention, the mouthwash is prepared from 1000 parts by weight of coconut water, 4-15 parts by weight of sodium hyaluronate, 80-120 mg of FE biological lysozyme with an enzyme activity of 8000-12000 units / mg, and an appropriate amount of preservative.

[0009] In a preferred embodiment of the present invention, the preservative is selected from potassium sorbate and / or sodium benzoate.

[0010] In a preferred embodiment of the present invention, the mouthwash does not contain ethanol, essence and / or sweetener.

[0011] The present invention relates to the use of the above-mentioned mouthwash in the preparation of oral care preparations for reducing dental plaque.

[0012] The present invention relates to the use of the above-mentioned mouthwash in the preparation of oral care preparations for preventing gum bleeding.

[0013] Another aspect of the present application relates to the use of the above-mentioned mouthwash in the preparation of an oral care preparation for preventing gingival inflammation.

[0014] Advantages

[0015] The formula of the present application helps to maintain the FE biological lysozyme activity and prolong the shelf life of the mouthwash. The mouthwash of the present application has certain effects on reducing dental plaque, gum bleeding and gingival inflammation. The present application uses natural coconut water instead of solvent water. The coconut water stock solution is rich in polysaccharides, proteins, organic acids, amino acids, trace elements and other functional ingredients, which can prolong the FE biological lysozyme activity of the prepared anhydrous mouthwash and has the effects of inhibiting harmful bacteria in the oral cavity, reducing oral odor and relieving gum problems. DETAILED DESCRIPTION

[0016] In order to further understand the present application, the technical solutions in the embodiments of the present application will be described clearly and completely below in conjunction with the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0017] Unless otherwise specified, the reagents involved in the embodiments of the present application are all commercially available and can be purchased through commercial channels.

[0018] Example 1:

[0019] Take 1000ml of coconut water, add 5g of sodium hyaluronate, stir uniformly, then add 100mg of FE biological lysozyme (enzyme activity is 10000 units / mg) and 1g of potassium sorbate, stir uniformly to obtain the anhydrous mouthwash of the present application.

[0020] Example 2:

[0021] Take 1000ml of coconut water, add 5g of sodium hyaluronate, stir uniformly, then add 100mg of FE biological lysozyme (enzyme activity is 10000 units / mg) and 3g of sodium benzoate, stir uniformly to obtain the anhydrous mouthwash of the present application.

[0022] Example 3:

[0023] Take 1000ml of coconut water, add 10g of sodium hyaluronate, stir uniformly, then add 100mg of FE biological lysozyme (enzyme activity is 10000 units / mg) and 1g of potassium sorbate, stir uniformly to obtain the anhydrous mouthwash of the present application.

[0024] Comparative Example 1:

[0025] Take 1000ml deionized water, add 10g sodium hyaluronate, stir evenly, then add 100mg FE bio-lysozyme (enzyme activity is 10000 units / mg) and 1g potassium sorbate, stir evenly.

[0026] Comparative Example 2:

[0027] Take 1000ml coconut water, add 5g sodium alginate, stir evenly, then add 100mg FE bio-lysozyme (enzyme activity is 10000 units / mg) and 1g potassium sorbate, stir evenly.

[0028] The above mouthwash is stored at room temperature for 1 month and 3 months, and the FE bio-lysozyme activity is determined according to the FE bio-lysozyme content detection method Q / 320281 NGS18-2014, and the test results are shown in Table 1.

[0029] Table 1 FE bio-lysozyme activity test results (n=3)

[0030] New preparation (x10 3 / ml) Save 1 month (x10 3 / ml) 3 months (x 10 3 / ml) Example 1 0.93±0.05 0.89±0.08 0.83±0.07 Example 2 0.94±0.08 0.88±0.06 0.84±0.08 Example 3 0.92±0.07 0.84±0.07 0.81±0.06 Comparative Example 1 0.97±0.09 0.72±0.06 0.51±0.04 Comparative Example 2 0.95±0.05 0.62±0.05 0.43±0.05

[0031] The above test results show that the use of the formula of the present application helps to maintain the activity of FE bio-lysozyme and prolong the shelf life of the mouthwash.

[0032] Example 4: Taking the application of the mouthwash prepared in Example 1 of the present application as an example, the clinical analysis results are as follows:

[0033] Test method: 80 people in the control group and 80 people in the experimental group, clinical examination after 1 month of use.

[0034] Control group: after meal, use warm water to rinse the mouth;

[0035] Experimental group: after meal, use the mouthwash of Example 1 of the present application to rinse the mouth;

[0036] The periodontal indexes of the subjects in the two groups before and after rinsing are shown in Table 2.

[0037]

[0038] The above test results show that the use of the mouthwash of the present application has very significant differences (p<0.01) in reducing plaque index (PLI), sulcus bleeding index (SBI) and gingival index (GI) compared with the control group. The test results show that the mouthwash of the present application has certain effect on reducing dental plaque, gingival bleeding and gingivitis.

[0039] The above describes the preferred embodiments of the present application, which is not intended to limit the present application. Those skilled in the art can make improvements and changes to the embodiments disclosed herein without departing from the scope and spirit of the present application.

Claims

1. A waterless mouthwash, wherein the mouthwash is prepared from 1000 parts by weight of coconut water, wherein 4-15 parts by weight of sodium hyaluronate, 80-120 mg of FE biological lysozyme with an enzyme activity of 8000-12000 units / mg and an appropriate amount of preservative are added; wherein the preservative is selected from potassium sorbate and / or sodium benzoate.

2. The mouthwash according to claim 1, wherein the mouthwash does not contain ethanol, flavoring and / or sweetener.

3. The use of the mouthwash according to claim 1 or 2 in the preparation of oral care formulations that reduce dental plaque.

4. The use of the mouthwash according to claim 1 or 2 in the preparation of oral care preparations for preventing gingival bleeding.

5. The use of the mouthwash according to claim 1 or 2 in the preparation of an oral care preparation for preventing gingivitis.

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