Method for detecting activation of basophilic granulocytes by inhaled allergens and use thereof
CN117783519BActive Publication Date: 2026-08-28SHANGHAI FIRST PEOPLES HOSPITAL
Patent Information
- Application Number
- CN202310649973.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Priority Date
- 2022-09-23
- Filing Date
- 2023-03-23
- Publication Date
- 2026-08-28
- Estimated Expiration
- 2043-03-23
AI Technical Summary
Technical Problem
但是，由于IgE的血清浓度很低(大约为IgG的3-7万分之一)，因此，检测难度大，且能够检测的过敏原有限(约30-40种)
Benefits of technology
[0140]本发明的主要优点
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Figure CN117783519B_ABST
Abstract
The present application relates to a kind of inhaled allergen basophil activation detection method and its application.Specifically, the present application provides a reagent combination for detection, which includes: a first antibody against human CD203c, a second antibody against human CD123, a third antibody against human CD45, a fourth antibody against human CD63, a positive control, and an inhaled allergen.The method of the present application can quickly and accurately detect basophil activation by blood collection, activation reagent configuration, activation, termination of activation, antibody labeling, red blood cell lysis, flow cytometry detection, result analysis and judgment.The present application can be used for diagnosing inhaled allergen sensitized allergic diseases, identifying inhaled allergen and its components, predicting the severity of allergy and monitoring the efficacy.
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Citation Information
Patent Citations
Kit for detecting basophil activation and using method of kit
CN104677810A
Allergy test based on flow cytometric analysis
US20100221756A1