A bactericidal composition containing Cyclobutrifluram and its application

Abstract

The present invention belongs to the field of pesticide sterilization technology and discloses a fungicide composition containing cyclobutrifluram and its use. The fungicide composition comprises active ingredient A and active ingredient B, wherein active ingredient A is cyclobutrifluram and active ingredient B is selected from one or more of pyraclostrobin and azoxystrobin. The mass ratio of active ingredient A to active ingredient B is 1:30 to 35:1. The fungicide composition of the present invention exhibits synergistic effects against various plant pathogens within a certain mass ratio range, can delay the development of drug resistance in pathogens, and has broad application prospects in production.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticide fungicides, and particularly relates to a fungicide composition containing Cyclobutrifluram and application thereof. Background Art

[0002] Cyclobutrifluram is a new nicotinamide fungicide and nematicide developed by Syngenta. It boasts a broad spectrum of efficacy, safety, and effectiveness against various nematodes and major fungal diseases. Direct application or seed treatment effectively controls root knots, beet cysts, and corn short-bodies in crops like cucumbers, tomatoes, corn, and sugar beets. It also effectively controls plant diseases, particularly Fusarium. Its CAS registration number is 1460292-16-3. Its chemical structure is as follows:

[0003]

[0004] Pyraclostrobin belongs to the methoxyacrylate fungicide class. It inhibits mitochondrial respiration, ultimately leading to cell death, exhibiting protective, therapeutic, and leaf-permeation effects. In addition to its direct effects on pathogens, pyraclostrobin can also induce physiological changes in many crops, particularly cereals, such as increasing nitrogen absorption, thereby promoting rapid growth and increasing yields.

[0005] Azoxystrobin is a novel methoxyacrylate fungicide that works by inhibiting mitochondrial respiration within bacterial cells, disrupting cellular energy synthesis and thereby inhibiting pathogenic bacterial spore germination, mycelial growth, and spore formation. Its high efficacy, broad spectrum, and low toxicity make it widely used to control diseases of various crops, including vegetables, fruit trees, cereals, and rice. It effectively controls a variety of fungal diseases, including leaf spot, anthracnose, powdery mildew, and downy mildew, while maintaining a high level of crop safety.

[0006] The applicant has conducted in-depth research on cyclobutrifluram with pyraclostrobin or azoxystrobin, and discovered that mixing cyclobutrifluram with pyraclostrobin or azoxystrobin, within a certain mixing ratio range, exhibits significant synergistic effects against peanut root rot, watermelon wilt, and wheat head blight, effectively improving the control of plant diseases. However, there are currently no reports on the use of cyclobutrifluram mixed with pyraclostrobin or azoxystrobin for the control of fungal diseases. Summary of the Invention

[0007] Based on the above problems, the present invention provides a fungicide composition containing Cyclobutrifluram. The fungicide composition can effectively control plant diseases, has a significant synergistic effect on the prevention of pathogens such as peanut root rot, watermelon wilt, and wheat fusarium wilt, can delay the development of drug resistance in pathogenic bacteria, and is conducive to the comprehensive management of agricultural diseases.

[0008] In order to achieve the above object, the present invention adopts the following technical solution: a fungicide composition containing Cyclobutrifluram, wherein the fungicide composition comprises an active ingredient A and an active ingredient B, wherein the active ingredient A is Cyclobutrifluram, and the active ingredient B is selected from one or more of pyraclostrobin and azoxystrobin;

[0009] A fungicidal composition containing Cyclobutrifluram, the fungicidal composition comprising active ingredient A and active ingredient B, wherein the active ingredient A is Cyclobutrifluram and the active ingredient B is pyraclostrobin;

[0010] Furthermore, the mass ratio of Cyclobutrifluram to pyraclostrobin is 1:30 to 35:1;

[0011] Furthermore, the mass ratio of Cyclobutrifluram to pyraclostrobin is 1:30 to 25:1;

[0012] Furthermore, the mass ratio of Cyclobutrifluram to pyraclostrobin is 1:20 to 16:1;

[0013] Furthermore, the mass ratio of Cyclobutrifluram to pyraclostrobin is 1:30, 1:20, 1:15, 1:10, 1:5, 1:1, 3:1, 8:1, 10:1, 15:1, 16:1, 25:1, and 35:1;

[0014] Furthermore, the mass ratio of Cyclobutrifluram to pyraclostrobin is 1:30, 1:20, 1:15, 1:10, 1:5, 1:1, 3:1, 8:1, 10:1, 15:1, 16:1, and 25:1;

[0015] A fungicidal composition containing Cyclobutrifluram, the fungicidal composition comprising active ingredient A and active ingredient B, wherein the active ingredient A is Cyclobutrifluram and the active ingredient B is azoxystrobin;

[0016] Furthermore, the mass ratio of Cyclobutrifluram to azoxystrobin is 1:20 to 20:1;

[0017] Furthermore, the mass ratio of Cyclobutrifluram to azoxystrobin is 1:20 to 10:1;

[0018] Furthermore, the mass ratio of Cyclobutrifluram to azoxystrobin is 1:10 to 1:5;

[0019] Furthermore, the mass ratio of Cyclobutrifluram to azoxystrobin is 1:20, 1:10, 1:8, 1:5, 1:3, 1:1, 10:1, and 20:1;

[0020] Furthermore, the mass ratio of Cyclobutrifluram to azoxystrobin is 1:10, 1:8, 1:5, 1:3, 1:1, and 10:1;

[0021] Furthermore, based on the total weight of the bactericidal composition being 100 wt%, the sum of the contents of the active ingredient A and the active ingredient B in the bactericidal composition is 1 to 90 wt%;

[0022] Preferably, the total content of the active ingredient A and the active ingredient B in the bactericidal composition is 5 to 80 wt%;

[0023] Furthermore, the fungicidal composition contains, in addition to the active ingredient, agriculturally acceptable auxiliary ingredients, wherein the auxiliary ingredients are selected from one or more of a wetting agent, a dispersant, an emulsifier, a thickener, a disintegrant, an antifreeze agent, a defoaming agent, a solvent, a preservative, a stabilizer, a synergist, a binder or a carrier;

[0024] Furthermore, the wetting agent is selected from one or more of alkylbenzene sulfonate, alkylnaphthalene sulfonate, lignin sulfonate, sodium lauryl sulfate, sodium dioctyl sulfosuccinate, α-olefin sulfonate, alkylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, alkylphenol ethoxylate, fatty alcohol ethoxylate, fatty alcohol polyoxyethylene ether sodium sulfate, silkworm feces, soapberry powder, soapberry powder, SOPA, detergent, emulsifier 2000 series and wetting penetrant F; and / or

[0025] Furthermore, the dispersant is selected from one or more of lignin sulfonates, alkylnaphthalene sulfonate formaldehyde condensates, naphthalene sulfonates, tristyrylphenol ethoxylate phosphates, fatty alcohol ethoxylates, alkylphenol polyoxyethylene ethers, alkylphenol polyoxyethylene ether methyl ether condensate sulfates, fatty amine polyoxyethylene ethers, glycerol fatty acid ester polyoxyethylene ethers, polycarboxylates, polyacrylic acids, phosphates, EO-PO block copolymers and EO-PO graft copolymers; and / or

[0026] Furthermore, the emulsifier is selected from one or more of calcium dodecylbenzenesulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenylethylphenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrylphenol polyoxyethylene ether, castor oil polyoxyethylene ether and alkylphenol ether phosphate; and / or

[0027] Furthermore, the thickener is selected from one or more of xanthan gum, organobentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose and white carbon black; and / or

[0028] Furthermore, the disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid and tartaric acid; and / or

[0029] Furthermore, the antifreeze is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons and inorganic salts; and / or

[0030] Furthermore, the defoaming agent is selected from C 10 -C 20 Saturated fatty acid compounds, silicone oil, silicone compounds, C8-C 10 One or more of fatty alcohols; and / or

[0031] Furthermore, the solvent is selected from one or more of benzene, toluene, xylene, durene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, alkylene carbonate, diesel, solvent oil, vegetable oil, vegetable oil derivatives and water; and / or

[0032] Furthermore, the preservative is selected from one or more of propionic acid, sodium propionate, sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate, methyl p-hydroxybenzoate, kasone and 1,2-benzisothiazolin-3-one; and / or

[0033] Furthermore, the stabilizer is selected from one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, bentonite, attapulgite, white carbon black, talc, montmorillonite and starch; and / or

[0034] Furthermore, the synergist is selected from synergist phosphine and piperonyl butoxide; and / or

[0035] Furthermore, the carrier is selected from one or more of ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils and vegetable oil derivatives.

[0036] The present invention optimizes the content of active ingredients and adjuvants in the pesticide composition to achieve a better balance between toxicity and residue, thereby enhancing efficacy, reducing dosage and lowering costs.

[0037] Furthermore, the dosage form of the bactericidal composition is selected from solid preparations and / or liquid preparations;

[0038] The solid preparations include powders, granules, pellets, tablets, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets or soluble granules;

[0039] The liquid preparations include soluble solutions, soluble gels, oils, film-spreading oils, emulsifiable concentrates, latexes, dispersible solutions, ointments, aqueous emulsions, oil emulsions, microemulsions, fats, suspensions, microcapsule suspensions, oil suspensions, dispersible oil suspensions, suspoemulsions, microcapsule suspension-suspension concentrates, microcapsule suspension-water emulsions, or microcapsule suspension-suspoemulsions;

[0040] Furthermore, the powder is a free-flowing powder preparation containing the active ingredient suitable for dusting or spreading;

[0041] Furthermore, the granules are free-flowing granular preparations containing active ingredients within a certain particle size range;

[0042] Furthermore, the spherical preparation is a spherical preparation (generally with a diameter greater than 6 mm) containing an active ingredient;

[0043] Furthermore, the tablet is a sheet-like preparation containing an active ingredient with a certain shape and size (usually having two flat or convex surfaces, with the distance between the two surfaces being less than the diameter);

[0044] Furthermore, the strips are strip- or rod-shaped preparations containing active ingredients (generally a few centimeters in length and a few millimeters in width / diameter, i.e., the length is greater than the diameter / width);

[0045] Furthermore, the wettable powder is a powdered preparation in which the active ingredient is dispersed into a suspension in water;

[0046] Furthermore, the oil-dispersible powder is a powdered preparation in which the active ingredient is dispersed into a suspension in an organic solvent;

[0047] Furthermore, the emulsion powder is a powdered preparation in which the active ingredient is dissolved in an organic solvent, encapsulated in a soluble or insoluble inert ingredient, and dispersed in water to form an oil-in-water emulsion;

[0048] Furthermore, the water dispersible granules are granular preparations that disintegrate in water and disperse the active ingredients into a suspension;

[0049] Furthermore, the emulsifiable granules are granular preparations in which the active ingredients are dissolved in an organic solvent, encapsulated in a soluble or insoluble inert ingredient, and dispersed in water to form an oil-in-water emulsion;

[0050] Furthermore, the water-dispersible tablet is a sheet-like preparation that disintegrates in water and disperses the active ingredients into a suspension;

[0051] Furthermore, the soluble powder is a powdered preparation in which the active ingredient forms a true solution in water and may contain inert ingredients that are insoluble in water;

[0052] Furthermore, the soluble granules are granular preparations in which the active ingredient forms a true solution in water and may contain inert ingredients that are insoluble in water;

[0053] Furthermore, the soluble tablet is a sheet-like preparation in which the active ingredient forms a true solution in water and may contain an inert ingredient that is insoluble in water;

[0054] Furthermore, the soluble preparation is a transparent or translucent liquid preparation containing an active ingredient that is diluted with water and may contain an inert component that is insoluble in water;

[0055] Furthermore, the soluble gel is a colloidal preparation containing active ingredients that is diluted with water to form a true solution;

[0056] Furthermore, the oil preparation is a liquid preparation diluted with an organic solvent (or not diluted) to form a homogeneous phase and containing an active ingredient;

[0057] Furthermore, the film-spreading oil is an oil containing active ingredients that automatically spreads on the water surface to form an oil film;

[0058] Furthermore, the emulsifiable concentrate is a homogeneous liquid preparation containing active ingredients that is dispersed into an emulsion by dilution with water;

[0059] Furthermore, the latex is a latex preparation containing active ingredients that is diluted and dispersed into an emulsion with water;

[0060] Furthermore, the dispersible liquid preparation is diluted with water and dispersed into a homogeneous liquid preparation containing the active ingredient in suspension;

[0061] Furthermore, the ointment is a water-based paste preparation containing active ingredients that can form a film and is generally used directly;

[0062] Furthermore, the aqueous emulsion is an emulsion liquid preparation in which the active ingredient (or its organic solution) is dissolved in water;

[0063] Furthermore, the oil emulsion is an emulsion liquid preparation in which the active ingredient (or its aqueous solution) is contained in oil;

[0064] Furthermore, the microemulsion is a transparent or translucent microemulsion liquid preparation in which the active ingredient is dissolved in water and is used directly or after being diluted with water;

[0065] Furthermore, the fat agent is an oil or fat-based viscous preparation containing active ingredients, which is generally used directly;

[0066] Furthermore, the suspension concentrate is a stable suspension liquid preparation in which the active ingredient is dispersed in water as solid particles, and is generally diluted with water for use;

[0067] Furthermore, the microcapsule suspension is a stable suspension liquid preparation formed by microcapsules containing active ingredients dispersed in a liquid;

[0068] Furthermore, the oil suspension concentrate is a stable suspension liquid preparation in which the active ingredient is dispersed in a liquid as solid particles, and is generally diluted with an organic solvent for use;

[0069] Furthermore, the dispersible oil suspension concentrate is a stable suspension liquid preparation in which the active ingredient is dispersed in a non-aqueous medium as solid particles, and is generally diluted with water for use;

[0070] Furthermore, the suspoemulsion is a heterogeneous liquid preparation in which the active ingredient is stably dispersed in the form of solid particles and water-insoluble tiny droplets in a continuous aqueous phase;

[0071] Furthermore, the microcapsule suspension-suspension concentrate is a stable suspension liquid preparation in which the active ingredient is dispersed in water in the form of microcapsules and solid particles;

[0072] Furthermore, the microcapsule suspension-water emulsion is a heterogeneous liquid preparation in which the active ingredients are stably dispersed in the form of microcapsules and tiny droplets in a continuous aqueous phase;

[0073] Furthermore, the microcapsule suspension-suspoemulsion is a heterogeneous liquid preparation in which the active ingredients are stably dispersed in the form of microcapsules, solid particles and tiny droplets in a continuous aqueous phase;

[0074] Furthermore, the fungicide composition can be prepared into a formulation acceptable for pesticides, and the formulation is a microemulsion, an aqueous emulsion, a suspension, a dispersible oil suspension, a soluble concentrate, an emulsifiable concentrate, a suspoemulsion, a microcapsule suspension, a water-dispersible granule, a wettable powder, a granule, a seed treatment suspension, or a seed treatment dry powder.

[0075] Furthermore, the formulation is in the form of a suspension, an aqueous emulsion, a water-dispersible granule, a wettable powder, a granule, or a seed treatment suspension.

[0076] The present invention also discloses the use of the bactericidal composition containing Cyclobutrifluram in preventing and controlling plant diseases.

[0077] Furthermore, the plant disease is a plant disease caused by fungi or bacteria;

[0078] Furthermore, the plant disease is a plant disease caused by fungi;

[0079] Furthermore, the plant disease is caused by pathogenic bacteria belonging to the genus Fusarium of the imperfect fungus;

[0080] Furthermore, the plant diseases are peanut root rot (Fusarium solan), watermelon wilt (Fusariumoxysporum f.sp.niveum), and wheat scab (Fusarium graminearum).

[0081] The beneficial effects of the present invention are as follows:

[0082] 1) The pesticide composition of the present invention has a significant effect on delaying the development of resistance in pathogens by rationally compounding active ingredients with different mechanisms of action;

[0083] 2) It is safe and efficient, safe to crops, non-target organisms, beneficial organisms and natural enemies, and has the effect of increasing production and ensuring income. DETAILED DESCRIPTION

[0084] In order to make the objects, advantages and technical solutions of the present invention more clearly understood, the present invention uses the following preparation examples and specific embodiments to explain the technical solutions of the present invention. However, the scope of protection of the present invention should not be limited to the specific implementation methods described herein.

[0085] Preparation example:

[0086] Preparation Example 1:

[0087] 22% Cyclobutrifluram·Pyraclostrobin SC (2:20)

[0088] Formula composition: Cyclobutrifluram 2%, pyraclostrobin 20%, sodium dioctyl sulfosuccinate 3%, sodium lignin sulfonate 1.2%, castor oil polyoxyethylene ether 1.2%, EO-PO block copolymer 2%, alkylphenol polyoxyethylene ether methyl ether condensate sodium sulfate 3%, sodium polycarboxylate 1.5%, silicone defoamer 1%, xanthan gum 0.2%, magnesium aluminum silicate 1%, ethylene glycol 5%, sodium benzoate 1%, and deionized water to make up the balance;

[0089] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in a reactor in sequence, water is added and mixed evenly, and the suspension product is obtained by high-speed shearing, wet sand grinding, and finally homogenization filtration.

[0090] Preparation Example 2:

[0091] 27% Cyclobutrifluram·Pyraclostrobin SC (24:3)

[0092] Formula composition: Cyclobutrifluram 24%, pyraclostrobin 3%, sodium lignin sulfonate 4.5%, castor oil polyoxyethylene ether 1.2%, EO-PO block copolymer 2%, alkylphenol polyoxyethylene ether methyl ether condensate sodium sulfate 3%, sodium polycarboxylate 1.5%, silicone defoamer 1%, xanthan gum 0.2%, magnesium aluminum silicate 1%, ethylene glycol 5%, sodium benzoate 1%, and deionized water to make up the balance;

[0093] Preparation method: Same as Preparation Example 1.

[0094] Preparation Example 3:

[0095] 18% Cyclobutrifluram·Azoxystrobin SC (2:16)

[0096] Formula composition: Cyclobutrifluram 2%, azoxystrobin 16%, sodium lauryl sulfate 4%, sodium lignin sulfonate 1.2%, castor oil polyoxyethylene ether 1.2%, potassium phosphate 1.5%, alkylphenol polyoxyethylene ether methyl ether condensate sodium sulfate 3%, sodium polycarboxylate 1.5%, silicone defoamer 1%, xanthan gum 0.2%, magnesium aluminum silicate 1%, ethylene glycol 5%, sodium benzoate 1%, and deionized water to make up the balance;

[0097] Preparation method: Same as Preparation Example 1.

[0098] Preparation Example 4:

[0099] 28% Cyclobutrifluram·Azoxystrobin SC (4:24)

[0100] Formula composition: Cyclobutrifluram 4%, azoxystrobin 24%, polycarboxylate 4%, sodium lignin sulfonate 4.5%, EO-PO block copolymer 2%, alkylphenol polyoxyethylene ether methyl ether condensate sodium sulfate 3%, sodium polycarboxylate 1.5%, silicone defoamer 1%, xanthan gum 0.2%, magnesium aluminum silicate 1%, propylene glycol 2%, glycerin 4%, carboxymethyl cellulose 0.8%, and deionized water makes up the balance;

[0101] Preparation method: Same as Preparation Example 1.

[0102] Preparation Example 5:

[0103] 50% Cyclobutrifluram·Pyraclostrobin Water Dispersible Granules (20:30)

[0104] Formula composition: Cyclobutriflura 20%, pyraclostrobin 30%, sodium lauryl sulfate 8%, naphthalenesulfonate formaldehyde condensate 8%, sodium polycarboxylate 2%, white carbon black 1%, kaolin makes up the balance;

[0105] Preparation method: According to the formula ratio of the embodiment, the active ingredient is added to the carrier, and the surfactant and other functional additives are added thereto, mixed, and after air flow grinding, 10-25% water is added, and then the water-dispersible granule product is obtained by kneading, granulating, drying and screening.

[0106] Preparation Example 6:

[0107] 45% Cyclobutrifluram·Azoxystrobin water dispersible granules (10:35)

[0108] Formula composition: Cyclobutriflura 10%, azoxystrobin 35%, sodium lauryl sulfate 7.5%, sodium lignin sulfonate 4%, naphthalene sulfonate formaldehyde condensate 4.3%, sodium polycarboxylate 2%, BX powder 4%, kaolin makes up the balance;

[0109] Preparation method: Same as Preparation Example 5.

[0110] Preparation Example 7:

[0111] 10% Cyclobutrifluram·Pyraclostrobin Emulsion in Water (4:6)

[0112] Formula composition: Cyclobutrifluram 4%, pyraclostrobin 6%, glycerol fatty acid ester polyoxyethylene ether 3%, polyoxyethylene sorbitan monooleate 3%, cyclohexanone 12%, xanthan gum 0.2%, ethylene glycol 5%, urea 1.5%, sodium sorbate 0.2%, silicone defoamer 0.4%, and deionized water to make up the balance;

[0113] Preparation method: According to the formula ratio of the embodiment, the active ingredient is dissolved in the solvent and an emulsifier is added to dissolve it into a uniform oil phase. Deionized water, antifreeze, etc. are mixed together to form a uniform water phase; under high-speed shear, the water phase is added to the oil phase to form a well-dispersed water emulsion product.

[0114] Preparation Example 8:

[0115] 50% Cyclobutrifluram·Azoxystrobin wettable powder (10:40)

[0116] Formula composition: Cyclobutrifluram 10%, azoxystrobin 40%, sodium lauryl sulfate 2.5%, sodium lignin sulfonate 10%, naphthalene sulfonate 1%, and kaolin to make up the balance;

[0117] Preparation method: pre-crush and mix evenly, then grind by air flow mill to a fineness that satisfies the requirement that at least 98 wt% passes through a 45 μm test sieve, to obtain the wettable powder product of the present invention.

[0118] Preparation Example 9:

[0119] 30% Cyclobutrifluram·Azoxystrobin seed treatment suspension concentrate (5:25)

[0120] Formula composition: Cyclobutrifluram 5%, azoxystrobin 25%, sodium lignin sulfonate 2.5%, isomeric tridecyl alcohol polyoxyethylene ether 2%, styrylphenol polyoxyethylene ether 2%, polyacrylic acid emulsion 1%, xanthan gum 0.2%, rose red pigment 5%, ethylene glycol 5%, magnesium aluminum silicate 0.8%, silicone defoamer 0.4%, sodium benzoate 1.2%, and deionized water to make up the balance;

[0121] Preparation method: According to the ratio, the active ingredient, adjuvant and water are mixed and stirred evenly through high shear, and then sanded for 2.5 hours in a sander to make the average particle size reach 1 to 5 microns to obtain the seed treatment suspension.

[0122] Preparation Example 10:

[0123] 40% Cyclobutrifluram. Azoxystrobin granules (5:35)

[0124] Formula composition: Cyclobutrifluram 5%, azoxystrobin 35%, methanol 5%, polyvinyl alcohol 2%, sodium alkylphenol sulfonate 10%, iron red 5%, bentonite 6%, starch 10%, river sand makes up the balance;

[0125] Preparation method: The active ingredient is dissolved in a solvent and then adsorbed and impregnated with sand particles as a carrier, and then an aqueous solution of a coating agent, a stabilizer, and a colorant is added for coating, and then dried to obtain granules.

[0126] Indoor toxicity

[0127] Method for determining the synergistic effect of mixing:

[0128] Sun Yunpei method: The synergistic effect of mixed drugs is evaluated based on the co-toxicity coefficient (CTC). A co-toxicity coefficient of CTC ≥ 120 indicates a synergistic effect; CTC ≤ 80 indicates an antagonistic effect; and 80 < CTC < 120 indicates an additive effect.

[0129] The co-toxicity coefficient (CTC value) of the mixture is calculated as follows:

[0130]

[0131] Where:

[0132] ATI - measured toxicity index of mixture;

[0133] S——EC of standard fungicide 50 , the unit is milligrams per liter (mg / L);

[0134] M——EC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0135] TTI=TI A *P A +TI B *P B

[0136] Where:

[0137] TTI – Theoretical Toxicity Index of Mixtures;

[0138] TI A ——Agent toxicity index;

[0139] P A ——The percentage of agent A in the mixture, in percentage (%);

[0140] TI B ——Toxicity index of agent B;

[0141] P B ——The percentage of agent B in the mixture, in percentage (%).

[0142]

[0143] Where:

[0144] CTC – Co-toxicity coefficient;

[0145] ATI - measured toxicity index of mixture;

[0146] TTI - Theoretical Toxicity Index of Mixture.

[0147] Example 1

[0148] Combined effects of a mixture of cyclobutrifluram and pyraclostrobin against peanut root rot pathogens

[0149] Test basis: Reference is made to the Agricultural Industry Standard of the People's Republic of China, Guidelines for Indoor Bioassay Tests of Pesticides - Fungicides Part 2: Test for Inhibition of Pathogenic Fungal Mycelial Growth, Plate Method NT / T 1156.2-2006.

[0150] Test agents: 96% Cyclobutrifluram technical and 97% Pyraclostrobin technical, provided by the Group's R&D Center.

[0151] Test pathogen: Peanut root rot fungus (Fusarium solan);

[0152] Preparation: Dissolve the test stock drug in acetone and then dilute with a 0.1% Tween 80 aqueous solution. Prepare a single-dose stock solution and set five concentration series based on the mixing purpose and drug activity. The final organic solvent content should not exceed 2%.

[0153] Melt the PDA culture medium in a microwave oven and cool it to about 50°C. Then, according to the principle of increasing the concentration from low to high, take 1 mL of the prepared drug solution and 9 mL of PDA culture medium and add them to a 9 cm diameter culture dish. Mix well to prepare drug-containing plates of corresponding concentrations.

[0154] The cultured pathogens were punched into a fungus cake using a 6 mm diameter puncher under sterile conditions. After the drug-containing culture medium solidified, the fungus cake was placed in the center of the culture medium. The culture dish was sealed with a sealing film and then placed in an incubator at 27°C for culture. A blank solution without drug was set up as a blank control. Each treatment was repeated four times.

[0155] After 4 days of culture, the colony diameters were measured with a caliper in millimeters (mm). Each colony was measured vertically once using the cross-hatch method, and the average value was taken.

[0156] The mycelial growth inhibition rate was calculated according to the following formula, and the unit was percentage (%), and the calculation result was rounded to two decimal places.

[0157] D=D1-D2

[0158] D——colony growth diameter;

[0159] D1 - colony diameter;

[0160] D2——diameter of mushroom cake.

[0161] I=(D0-D t ) / D0*100

[0162] Where:

[0163] I——hyphae growth inhibition rate;

[0164] D0——blank control colony growth diameter;

[0165] D t ——The growth diameter of the colony after treatment with chemicals.

[0166] Statistical analysis: DPS data processing software was used to calculate the test results and to determine the toxicity regression equation, R, and EC of the test agent alone and the mixtures of different proportions. 50 and EC 90 As well as the 95% confidence limit, calculate the co-toxicity coefficient (CTC) of different ratios of the two drugs.

[0167] Test results:

[0168] Table 1 Indoor activity test of Cyclobutrifluram and pyraclostrobin mixture against peanut root rot pathogen

[0169]

[0170] From the indoor activity test (see Table 1), Cyclobutrifluram and pyraclostrobin mixed with peanut root rot fungus EC 50 The concentrations of Cyclobutrifluram and pyraclostrobin were 0.084 mg / L and 8.048 mg / L respectively. Peanut root rot fungi were more sensitive to Cyclobutrifluram. When Cyclobutrifluram and pyraclostrobin were mixed in a ratio of 1:30 to 25:1, the co-toxicity coefficient was greater than 120, showing a synergistic effect in preventing and controlling peanut root rot fungi.

[0171] Example 2:

[0172] Combined effects of cyclobutrifluram and pyraclostrobin mixture against watermelon wilt pathogen

[0173] Test basis: Refer to the Agricultural Industry Standard of the People's Republic of China, Guidelines for Indoor Bioassay Test of Pesticides, Fungicides Part 2: Test for Inhibition of Pathogenic Fungal Mycelial Growth, Plate Method NT / T 1156.2-2006.

[0174] Test agents: 96% Cyclobutrifluram technical and 97% Pyraclostrobin technical, provided by the Group's R&D Center.

[0175] Test pathogen: Watermelon wilt pathogen (Fusarium oxysporum f.sp.niveum);

[0176] Preparation: Dissolve the test stock drug in acetone and then dilute with a 0.1% Tween 80 aqueous solution. Prepare a single-dose stock solution and set five concentration series based on the mixing purpose and drug activity. The final organic solvent content should not exceed 2%.

[0177] Melt the PDA culture medium in a microwave oven and cool it to about 50°C. Then, according to the principle of increasing the concentration from low to high, take 1 mL of the prepared drug solution and 9 mL of PDA culture medium and add them to a 9 cm diameter culture dish. Mix well to prepare drug-containing plates of corresponding concentrations.

[0178] The cultured pathogens were punched into a fungus cake using a 6 mm diameter puncher under sterile conditions. After the drug-containing culture medium solidified, the fungus cake was placed in the center of the culture medium. The culture dish was sealed with a sealing film and then placed in an incubator at 27°C for culture. A blank solution without drug was set up as a blank control. Each treatment was repeated four times.

[0179] After 8 days of culture, the colony diameters were measured with a caliper in millimeters (mm). Each colony was measured vertically once using the cross-hatch method, and the average value was taken.

[0180] The mycelial growth inhibition rate was calculated according to the following formula, and the unit was percentage (%), and the calculation result was rounded to two decimal places.

[0181] D=D1-D2

[0182] D——colony growth diameter;

[0183] D1 - colony diameter;

[0184] D2——diameter of mushroom cake.

[0185] I=(D0-D t ) / D0*100

[0186] Where:

[0187] I——hyphae growth inhibition rate;

[0188] D0——blank control colony growth diameter;

[0189] D t ——The growth diameter of the colony after treatment with chemicals.

[0190] Statistical analysis: DPS data processing software was used to calculate the test results and to determine the toxicity regression equation, R, and EC of the test agent alone and the mixtures of different proportions. 50 and EC 90 As well as the 95% confidence limit, calculate the co-toxicity coefficient (CTC) of different ratios of the two drugs.

[0191] Test results:

[0192] Table 2 Indoor activity test of Cyclobutrifluram and pyraclostrobin mixture against watermelon wilt pathogen

[0193]

[0194]

[0195] From the indoor activity test (see Table 2), Cyclobutrifluram and pyraclostrobin mixed with watermelon wilt fungus EC 50 The concentrations of Cyclobutrifluram and pyraclostrobin were 0.021 mg / L and 2.556 mg / L respectively. Watermelon wilt pathogen was more sensitive to Cyclobutrifluram. The co-toxicity coefficient of Cyclobutrifluram and pyraclostrobin was greater than 120 when mixed in a ratio of 1:30 to 15:1, showing a synergistic effect in preventing and controlling watermelon wilt.

[0196] Example 3:

[0197] Combined effects of cyclobutrifluram, pyraclostrobin and azoxystrobin on wheat fusarium head blight

[0198] Test basis: Reference is made to the Agricultural Industry Standard of the People's Republic of China, Guidelines for Indoor Bioassay Tests of Pesticides - Fungicides Part 2: Test for Inhibition of Pathogenic Fungal Mycelial Growth, Plate Method NT / T 1156.2-2006.

[0199] Test agents: 96% Cyclobutrifluram technical, 97% Pyraclostrobin technical, 97% Azoxystrobin technical, provided by the Group's R&D Center.

[0200] Test pathogen: wheat fusarium (Fusarium graminearum);

[0201] Preparation: Dissolve the test stock drug in acetone and then dilute with a 0.1% Tween 80 aqueous solution. Prepare a single-dose stock solution and set five concentration series based on the mixing purpose and drug activity. The final organic solvent content should not exceed 2%.

[0202] Melt the PDA culture medium in a microwave oven and cool it to about 50°C. Then, according to the principle of increasing the concentration from low to high, take 1 mL of the prepared drug solution and 9 mL of PDA culture medium and add them to a 9 cm diameter culture dish. Mix well to prepare drug-containing plates of corresponding concentrations.

[0203] The cultured pathogens were punched into a fungus cake using a 6 mm diameter puncher under sterile conditions. After the drug-containing culture medium solidified, the fungus cake was placed in the center of the culture medium. The culture dish was sealed with a sealing film and then placed in an incubator at 27°C for culture. A blank solution without drug was set up as a blank control. Each treatment was repeated four times.

[0204] After 8 days of culture, the colony diameters were measured with a caliper in millimeters (mm). Each colony was measured vertically once using the cross-hatch method, and the average value was taken.

[0205] The mycelial growth inhibition rate was calculated according to the following formula, and the unit was percentage (%), and the calculation result was rounded to two decimal places.

[0206] D=D1-D2

[0207] D——colony growth diameter;

[0208] D1 - colony diameter;

[0209] D2——diameter of mushroom cake.

[0210] I=(D0-D t ) / D0*100

[0211] Where:

[0212] I——hyphae growth inhibition rate;

[0213] D0——blank control colony growth diameter;

[0214] D t ——The growth diameter of the colony after treatment with chemicals.

[0215] Statistical analysis: DPS data processing software was used to calculate the test results and to determine the toxicity regression equation, R, and EC of the test agent alone and the mixtures of different proportions. 50 and EC 90 As well as the 95% confidence limit, calculate the co-toxicity coefficient (CTC) of different ratios of the two drugs.

[0216] Test results:

[0217] Table 3 Indoor activity test of Cyclobutrifluram and pyraclostrobin mixture against wheat fusarium

[0218]

[0219] From the indoor activity test (see Table 3), Cyclobutrifluram and pyraclostrobin mixed with wheat fusarium EC 50 The concentrations of cyclobutrifluram and pyraclostrobin were 2.126 mg / L and 0.685 mg / L respectively. Wheat fusarium serovar was more sensitive to pyraclostrobin. The co-toxicity coefficient of cyclobutrifluram and pyraclostrobin was greater than 120 when mixed in a ratio of 1:15 to 16:1, which showed a synergistic effect in the prevention and control of wheat fusarium serovar.

[0220] Table 4 Indoor activity test of Cyclobutrifluram and azoxystrobin mixture against wheat fusarium

[0221]

[0222] From the indoor activity test (see Table 4), Cyclobutrifluram and azoxystrobin mixed with wheat fusarium EC 50 The concentrations of cyclobutrifluram and azoxystrobin were 2.126 mg / L and 15.265 mg / L respectively. Wheat fusarium rust was more sensitive to azoxystrobin. The co-toxicity coefficient of cyclobutrifluram and azoxystrobin was greater than 120 when mixed in a ratio of 1:20 to 20:1, showing a synergistic effect in the prevention and control of wheat fusarium rust.

[0223] Field efficacy

[0224] Example 4:

[0225] Field efficacy of a mixture of cyclobutrifluram and pyraclostrobin in controlling wheat scab

[0226] The test varieties: Wheat, variety Luyuan 502, is in the early flowering stage and is growing well;

[0227] Target of prevention and control: wheat scab;

[0228] The test location: Laixi City, Qingdao City, Shandong Province, and the application time is early May 2022.

[0229] Experimental method: Random block arrangement was used, with a plot area of 30m 2 , repeat 4 times.

[0230] Application method and frequency: At the early stage of wheat flowering, stem and leaf spraying was used with a spray volume of 450 L / hectare. In this experiment, the first application of pesticide was at the early stage of wheat flowering (May 8), and the second application was on May 15.

[0231] Investigation method: The control effect was investigated from the milky stage of wheat to 10 days before harvest (May 25).

[0232] Survey Method: Refer to the "Guidelines for Field Efficacy Tests of Pesticides, Part 15: Control of Wheat Scab with Fungicides (NY / T1464.15-2007)." Samples were collected at five diagonal points within each plot, with 200 ears surveyed at each point. Grades were determined based on the percentage of dead ear area to total ear area, and the number of diseased ears at each grade and the total number of ears were recorded.

[0233] Grading method:

[0234] Level 0: The whole ear is disease-free;

[0235] Level 1: The dead ear area accounts for less than 1 / 4 of the entire ear area;

[0236] Level 3: The dead ear area accounts for 1 / 4 to 1 / 2 of the entire ear area;

[0237] Level 5: The dead ear area accounts for 1 / 2 to 3 / 4 of the entire ear area;

[0238] Level 7: The dead ear area accounts for more than 3 / 4 of the entire ear area.

[0239] The efficacy is calculated as follows:

[0240] X=Σ(N i ×i) / (N×7)×100

[0241] Where:

[0242] X—disease index;

[0243] Ni—number of diseased ears at each level;

[0244] i—relative magnitude value;

[0245] N—total number of ears surveyed.

[0246] The control effect is calculated according to the following formula:

[0247] P=(X1-X2) / X1×100

[0248] Where:

[0249] P—control effect, in percentage (%);

[0250] X1—disease index of blank control area;

[0251] X2—Disease index of the drug-treated area.

[0252] Results and Analysis:

[0253] Table 5 Field efficacy of cyclobutrifluram mixed with pyraclostrobin and azoxystrobin in controlling wheat fusarium head blight

[0254]

[0255] Note: The control effect percentage in the above table is the average value of each replicate; lowercase letters represent significant differences at the 5% level, and uppercase letters represent significant differences at the 1% level.

[0256] Field efficacy showed (see Table 5): Under the same environmental conditions, the overall efficacy of different treatments in controlling wheat scab from the milky stage to 10 days before harvest was 72.79% to 90.20%.

[0257] The control effects of the mixed preparations 22% Cyclobutrifluram·pyraclostrobin suspension concentrate (2:20), 27% Cyclobutrifluram·pyraclostrobin suspension concentrate (24:3), 50% Cyclobutrifluram·pyraclostrobin water dispersible granules (20:30), and 10% Cyclobutrifluram·pyraclostrobin emulsion in water (4:6) were 85.33%, 90.20%, 86.95%, and 86.09%, respectively. The control efficacy of 18% Cyclobutrifluram·Azoxystrobin SC (2:16), 28% Cyclobutrifluram·Azoxystrobin SC (4:24), 45% Cyclobutrifluram·Azoxystrobin WDG (10:35), and 50% Cyclobutrifluram·Azoxystrobin WP (10:40) against wheat head blight was 85.99%, 88.07%, 86.45%, and 82.13%, respectively. The mixed formulations were significantly more effective than the control single agents of 450 g / L cyclobutrifluram SC, 25% pyraclostrobin SC, and 250 g / L azoxystrobin SC against wheat head blight.

[0258] Example 5:

[0259] Field efficacy of a mixture of cyclobutrifluram and azoxystrobin in controlling peanut root rot

[0260] Varieties tested in the experiment: Peanut (Baisha);

[0261] Target of prevention and control: Peanut root rot;

[0262] The test location: Laixi City, Qingdao City, Shandong Province, and the application time is early June 2022.

[0263] Experimental method: Random block arrangement was used, with a plot area of 30m 2 , repeat 4 times.

[0264] Application method and water consumption: The application method is seed coating. The seeds are coated before peanut planting and sowing. After drying, sowing is done. The water consumption is 2.5 liters of water for every 100 kg of seeds, and the sowing amount per mu is 15 kg.

[0265] Survey Method: Survey the emergence rate of peanuts at the same time as the seedlings are fully planted after sowing; survey the incidence of root rot 60 days after sowing. Surveys were conducted twice in total.

[0266] Seedling emergence rate survey: In each plot, the number of peanut seedlings emerging was investigated for 1,000 pieces, and the peanut emergence rate was calculated.

[0267] For the disease index survey, 5 random sampling points were selected in each plot, 100 plants were surveyed at each point, and 500 plants were surveyed in each plot. The number of diseased peanut plants at all levels was recorded, and the disease index and control effect were calculated.

[0268] Disease classification standards:

[0269] Level 0: There are no lesions on the stem base and main root of the plant;

[0270] Level 1: There are a few lesions on the stem base and main root;

[0271] Level 3: There are many lesions on the stem base and main root, and the area of lesions accounts for 25% to 50% of the total area of the stem and root;

[0272] Level 5: There are many large lesions on the stem base and main root, and the lesion area accounts for 50% to 75% of the total area of the stem and root;

[0273] Level 7: The lesions are continuous at the base of the stem or on the main root, forming a stem-circling phenomenon, but the root system is not dead;

[0274] Level 9: Root necrosis, above-ground part of the plant wilts or dies.

[0275] The efficacy is calculated as follows:

[0276] Seedling emergence rate (%) = number of seedlings emerged / number of seeds sown × 100

[0277] Disease index = Σ(number of diseased plants at each level × relative level value) / (total number of plants surveyed × 9) × 100

[0278] Control effect (%) = (disease index of blank control area - disease index of treated area) / disease index of blank control area × 100

[0279] Results and Analysis:

[0280] Table 6 Determination of peanut emergence rate by mixing Cyclobutrifluram and azoxystrobin

[0281]

[0282]

[0283] Note: The percentage of protective effect in the above table is the average value of each replicate; lowercase letters represent significant differences at the 5% level.

[0284] Table 7 Field efficacy of cyclobutrifluram and azoxystrobin mixed to control peanut root rot

[0285]

[0286] Note: The percentage of protective effect in the above table is the average value of each replicate; lowercase letters represent significant differences at the 5% level, and uppercase letters represent significant differences at the 1% level.

[0287] From the results of field trial emergence rate in Table 6, it can be seen that the average emergence rate of peanuts treated with 30% Cyclobutrifluram·Azoxystrobin seed treatment suspension concentrate (5:25) at 20, 30, and 40 g / 100 kg of seeds was not significantly different from that of the water control.

[0288] From the field test results in Table 7, it can be seen that the results of the seedling emergence rate test of the test agent show that the test agent 30% Cyclobutrifluram·Myclobutanil seed treatment suspension concentrate (5:25) has a good control effect on peanut root rot. 60 days after sowing, the average control effect of peanut root rot when applying 40 grams of active ingredient / 100 kilograms of seeds is 90.46%, showing excellent control effect, which is significantly higher than the control single agent.

[0289] Example 6:

[0290] Field efficacy of a mixture of cyclobutrifluram and azoxystrobin in controlling watermelon wilt

[0291] Test varieties: Watermelon, the variety is Fengle Little Angel;

[0292] Target of prevention and control: Watermelon wilt;

[0293] The test location: Laixi City, Qingdao City, Shandong Province, and the application time is mid-June 2022.

[0294] Experimental method: Random block arrangement was used, with a plot area of 30m 2 , repeat 4 times.

[0295] Application method and frequency: In the early stage of the disease, the application method is broadcasting; in this experiment, the first application was made at the early stage of watermelon wilt disease (June 10), and the second application was made on June 24.

[0296] Survey Methods: Refer to "Guidelines for Field Efficacy Tests of Pesticides, Part 113: Control of Fusarium Wilt in Cucurbits with Fungicides (GB / T17980.113-2004)." Investigate all plants in the plot for typical symptoms of wilt, recording the number of plants affected and the total number of plants surveyed. Efficacy was assessed before the next application and 7 days after the last application.

[0297] The efficacy is calculated as follows:

[0298] Disease rate = number of diseased plants / total number of plants surveyed × 100

[0299] Control effect = (disease rate in blank control area - disease rate in treated area) / disease rate in blank control area × 100

[0300] Results and Analysis:

[0301] Table 8 Field efficacy of Cyclobutrifluram and azoxystrobin mixed to control watermelon wilt

[0302]

[0303] The field efficacy of a mixture of Cyclobutrifluram and azoxystrobin in controlling watermelon wilt was shown in Table 8: 7 days before the second application, 40% Cyclobutrifluram and azoxystrobin granules (5:35) were used at a dosage of 100 g / hm2. 2 、150g / hm 2 , 200g / hm 2 The control effects on watermelon wilt were 80.85%, 85.11% and 87.23% respectively, which were significantly higher than those of 20% cyclobutrifluram granules and 1% azoxystrobin granules.

[0304] Seven days after the last application, the overall control efficacy of each treatment field was 77.46% to 90.14%, and 40% Cyclobutrifluram. Azoxystrobin granules (5:35) 100g / hm 2 、150g / hm 2 , 200g / hm 2 The control rates of watermelon wilt disease after treatment with three different doses were 83.10%, 87.32% and 90.14% respectively.

[0305] Therefore, compounding cyclobutrifluram with pyraclostrobin and myclobutanil in a specific ratio can alleviate the increasing trend of resistance to peanut root rot, watermelon wilt and wheat ergot, improve the effect of disease prevention and control and extend the service life of the agent.

[0306] Although the present application describes specific embodiments in detail by way of example, the disclosure of the present application may adopt various modifications and alternative forms. However, it should be understood that the disclosure of the present application is not limited to the specific forms disclosed. On the contrary, the disclosure of the present application covers all modifications, equivalents and alternative forms within the scope of the disclosure of the present application, and the scope of the present application is limited by the appended claims and their legal equivalents.

Claims

1. A bactericidal composition containing Cyclobutrifluram, characterized in that The bactericidal composition consists of an active ingredient A and an active ingredient B, wherein the active ingredient A is Cyclobutrifluram, the active ingredient B is pyraclostrobin, and the mass ratio of Cyclobutrifluram to pyraclostrobin is 1:30 to 25:

1.

2. The bactericidal composition according to claim 1, characterized in that The mass ratio of Cyclobutrifluram to pyraclostrobin is 1:20-16:

1.

3. The bactericidal composition according to claim 1, characterized in that Based on the total weight of the bactericidal composition being 100 wt %, the sum of the contents of the active ingredient A and the active ingredient B in the bactericidal composition is 1 to 90 wt %.

4. The bactericidal composition according to claim 3, characterized in that The total content of the active ingredient A and the active ingredient B in the bactericidal composition is 5 to 80 wt%.

5. The bactericidal composition according to claim 1, characterized in that In addition to the active ingredients, the fungicidal composition also contains agriculturally permitted auxiliary ingredients, and the auxiliary ingredients are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoaming agents, solvents, preservatives, stabilizers, synergists, binders or carriers.

6. The bactericidal composition according to claim 5, characterized in that The dosage form of the bactericidal composition is selected from solid preparations and / or liquid preparations.

7. The bactericidal composition according to claim 6, characterized in that The fungicide composition can be prepared into a formulation acceptable for pesticides, and the formulation is a microemulsion, an aqueous emulsion, a suspension, a dispersible oil suspension, a soluble solution, an emulsifiable concentrate, a suspoemulsion, a microcapsule suspension, a water-dispersible granule, a wettable powder, a granule, a seed treatment suspension, or a seed treatment dry powder.

8. The bactericidal composition according to claim 7, characterized in that The preparation dosage forms are suspension, aqueous emulsion, water dispersible granule, wettable powder, granule and seed treatment suspension.

9. Use of the fungicidal composition according to any one of claims 1 to 8 in preventing and controlling plant diseases, characterized in that: The plant diseases are peanut root rot, watermelon wilt and wheat fusarium wilt.

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