A method of determining the health of a flock of chickens

Abstract

The application discloses a method for determining the health degree of chicken population, and specifically comprises the following steps: step one, preparing the breeding cage and the excrement removing device; step two, selecting the chicken population with appropriate age, health, uniformity of body weight greater than or equal to 85% and body temperature within the normal temperature range; step three, effectively implementing the predetermined test plan and test additive; step four, counting the excrement quantity by drawing lines on the tray, using the function method to calculate the change rate, and performing comparative analysis; and step five, using the area statistical mean method to calculate the mean value after comparison. The application is a method for determining the health degree of test chicken population, and has the characteristics of simplicity, rapidness and high efficiency. The method can realize the evaluation of the intestinal tract and excrement of the chicken population through data, and achieves the simple and intuitive data technology effect. The method can also be applied to large-scale chicken population.

Description

Technical Field

[0001] This invention relates to the field of poultry and livestock breeding technology, and in particular to a method for determining the health of a flock of chickens. Background Technology

[0002] Chicken farming is an important part of agricultural aquaculture. It involves using methods such as raising chickens in mountainous areas, in plastic greenhouses, and in cages to utilize the chickens' physiological functions. Through artificial feeding and breeding, the chickens transform plant-based feed into productive animal products, yielding chicken meat and eggs. In the process of raising chickens, it is essential to regularly observe the flock's health and make timely assessments to prevent the persistent occurrence of chicken diseases.

[0003] Currently, with the development of integrated farming technology, subclinical symptoms are becoming more and more common in broiler farming, making diagnosis increasingly difficult, especially given the rapid progression of diseases and the difficulty in observation. In production, scientific research experiments place more emphasis on health, but the apparent condition is not reflected in data, is not obvious enough, and is also influenced by many subjective factors. Summary of the Invention

[0004] This invention discloses a method for determining the health of a chicken flock, aiming to address the technical problems mentioned in the background art. With the development of integrated farming technology, subclinical symptoms in broiler farming are becoming more and more common, making diagnosis increasingly difficult, especially when the disease progresses rapidly and is not easy to observe. In clinical practice, scientific research experiments pay more attention to health, but the apparent state is not reflected by data and is not obvious enough. There are also many subjective factors that influence the evaluation.

[0005] To achieve the above objectives, the present invention adopts the following technical solution:

[0006] A method for determining the health of a flock of chickens includes the following steps:

[0007] Step 1: Selection of a standard 1-day-old flock. Select a flock with a weight uniformity of ≥85%. Measure the body temperature of the newborn chicks (temperature difference less than 0.3 degrees Celsius, good and consistent activity levels, ensuring consistent health).

[0008] Step 2: Test chick parent stock: high uniformity, thorough eradication of pullorum disease and fowl leukosis, ensuring that commercial broiler chicks are free from vertically transmitted diseases.

[0009] Step 3: Carry out feeding and disease prevention according to the predetermined experiment;

[0010] Step 4: The experiment was divided into groups A, B, C, D and E. The number of chickens was the same and each group had at least 3 replicates. Each replicate was cage-housed. This grouping principle is consistent with the arbitrary feeding experiment.

[0011] Step 5: Different additives were selected in the experiment, but the same addition method was used. This group selected licorice extract, eucommia extract, fermented natural plant complex, and compound organic acid to verify the effects of these four substances on the health of the chicken flock. A means no feeding of the above substances, and B, C, D, and E are the four substances mentioned above.

[0012] Step Six: At 6 PM the day before, prepare clean manure trays (80cm*80cm) for each group of babies at 1 day, 7 days, 14 days, 28 days, 35 days, and 42 days of age. Place the trays under the cages of each group and each set. Line the trays with ordinary graph paper (80cm*80cm) and attach a transparent plastic film tightly to the paper to ensure cleanliness. Label the trays and record the contents.

[0013] Step 7: At 6:00 AM on the second day, count the feces in the feces tray, following the principle of counting from top to bottom and from left to right. Count the number of colored feces, the number of loose feces, and the number of consecutive loose feces, and then summarize the results.

[0014] Step 8: After the count is completed at 6:00 AM, the feces are removed, and a new liner is laid down. The count is repeated at 6:00 PM. Similarly, the count is carried out on the designated dates of the experiment according to the plan.

[0015] Step 9: After counting all 6 times, organize the data, and each group corresponds to a sparse function curve;

[0016] Step 10: By comparing the slopes of the function trend lines, the relationship between the rate of change and age can be derived, which helps to explain which flock of chickens is healthier.

[0017] Step Eleven: Using the same steps as Steps One, Two, Three, and Four, and proceeding in parallel with Steps Five, Six, Seven, Eight, and Nine, prepare clean manure trays (80cm*80cm) at 6 PM the day before, at 1 day, 7 days, 14 days, 28 days, 35 days, and 42 days of age. Place these trays under the selected empty cages for each group. Line the manure trays with 80cm*80cm filter paper to ensure cleanliness, and label and record the trays. At the same time, select one chicken of the same size (with minimal weight difference) from each repeat in each group and keep it in an empty cage for manure observation.

[0018] Step 12: For each group and each replicate, the fecal area was measured and statistically analyzed at 6:00 AM on the second day. The procedure was as follows: the filter paper for each replicate was taken out at the same time, then placed in the air to dry for 1 hour, and the x-axis and y-axis were marked with a ruler. The diffusion area was then measured and statistically analyzed, and the fecal weight was also statistically analyzed. The results were then summarized.

[0019] Step Thirteen: In the statistical experiment, compare the sum of the areas of water diffusion in the feces of each chicken in each replicate with the percentage of the total weight of the feces of that chicken. This comparison can be made horizontally or vertically.

[0020] By evaluating the health of chicken flocks, specifically by statistically comparing the rate of loose stools and the fecal diffusion ratio, the SS flock identification method can be used. This method is applicable to both experimental and production flocks and can provide a reference for assessing the intestinal health of chicken flocks and comparing additives in both experimental and production settings.

[0021] In a preferred embodiment, the selection of the chicken flock in step one includes the following conditions: selection of a standard 1-day-old chicken flock: selecting a flock with a weight uniformity ≥85%, and measuring the body temperature of the newborn chickens (the body temperature difference is less than 0.3 degrees Celsius, the chickens are active and uniform, ensuring consistent health); experimental ventilation: the minimum ventilation for the first 5 days, the transitional ventilation for 6-21 days, and the variable ventilation for 22-42 days based on actual conditions (depending on seasonal changes); the chicken house is a standard closed chicken house, the lighting varies according to the age of the chickens, the feed is early stage (1-21 days) feed and late stage (22-42 days) feed, the drinking water is conventional drinking water and the water pressure is controlled according to the age, and the feeding program increases according to the standard with the age.

[0022] In a preferred embodiment, the fermented natural plant is a crude extract of Codonopsis pilosula and Astragalus membranaceus, and the complex organic acid is a hexacarboxylic acid consisting of formic acid, propionic acid, phosphoric acid, lactic acid, benzoic acid, and citric acid. In step three, during the rearing process, the indoor temperature should be maintained at 33-35℃ for the first week, decreasing by 2-3℃ each week thereafter until it reaches 21℃. Observe the flock's spirit, appetite, and droppings during morning and evening feedings. Ensure regular ventilation in the chicken house to maintain fresh air and prevent ammonia poisoning. Avoid contaminating feed troughs and water troughs with droppings, and frequently clean and disinfect all equipment. Follow the farm's immunization schedule: Marek's disease vaccine at 1 day old; Newcastle disease and infectious bronchitis bivalent vaccine in drinking water at 7 days old; infectious bursal disease vaccine orally at 12 days old, and H5N1 influenza oil vaccine subcutaneously at 12 days old; Newcastle disease four-strain and infectious bronchitis bivalent vaccine via nasal and ocular drops at 21 days old; infectious bursal disease vaccine in drinking water at 28 days old; and Newcastle disease I strain intramuscular injection at 50 days old.

[0023] The feed formula used in chicken farming is scientific, rich in nutrients, and has a comprehensive composition. The feed pellets have excellent appearance quality, low powder content, and high quality, which can effectively improve the accuracy of the experiment.

[0024] In a preferred embodiment, before raising the chickens, the chicken house needs to be mechanically disinfected, including removing chicken droppings, dust from the roof and walls, rinsing the chicken house, and then chemically disinfecting it. This involves rinsing feed troughs and water jugs with a commercially available disinfectant solution, followed by soaking them in disinfectant water for 12-24 hours, spraying the entire chicken house with disinfectant, and fumigating with a 2:1 solution of formaldehyde and potassium permanganate. Maintain a temperature of 20-22°C and humidity of 55-60%. Ventilation should begin one week before the chicks arrive. Simultaneously, the equipment in the chicken house should be inspected and repaired, the chicken house should be tidied, and dry bedding paper should be laid. The bedding paper must be disinfected before use, and any broken bedding paper should be replaced immediately.

[0025] As shown above, a method for determining the health of a chicken flock includes the following steps: Step 1: Selection of a routine 1-day-old chicken flock, selecting a flock with a weight uniformity ≥85%, and measuring the body temperature of the newborn chickens (temperature difference less than 0.3 degrees Celsius, good and consistent activity level, ensuring consistent health);

[0026] Step 2: Test chick parent stock: high uniformity, thorough eradication of pullorum disease and fowl leukosis, ensuring that commercial broiler chicks are free from vertically transmitted diseases.

[0027] Step 3: Carry out feeding and disease prevention according to the predetermined experiment;

[0028] Step 4: The experiment was divided into groups A, B, C, D and E. The number of chickens was the same and each group had at least 3 replicates. Each replicate was cage-housed. This grouping principle is consistent with the arbitrary feeding experiment.

[0029] Step 5: Different additives were selected in the experiment, but the same addition method was used. This group selected licorice extract, eucommia extract, fermented natural plant complex, and compound organic acid to verify the effects of these four substances on the health of the chicken flock. A means no feeding of the above substances, and B, C, D, and E are the four substances mentioned above.

[0030] Step Six: At 6 PM the day before, prepare clean manure trays (80cm*80cm) for each group of babies at 1 day, 7 days, 14 days, 28 days, 35 days, and 42 days of age. Place the trays under the cages of each group and each set. Line the trays with ordinary graph paper (80cm*80cm) and attach a transparent plastic film tightly to the paper to ensure cleanliness. Label the trays and record the contents.

[0031] Step 7: At 6:00 AM on the second day, count the feces in the feces tray, following the principle of counting from top to bottom and from left to right. Count the number of colored feces, the number of loose feces, and the number of consecutive loose feces, and then summarize the results.

[0032] Step 8: After the count is completed at 6:00 AM, the feces are removed, and a new liner is laid down. The count is repeated at 6:00 PM. Similarly, the count is carried out on the designated dates of the experiment according to the plan.

[0033] Step 9: After counting all 6 times, organize the data, and each group corresponds to a sparse function curve;

[0034] Step 10: By comparing the slopes of the function trend lines, the relationship between the rate of change and age can be derived, which helps to explain which flock of chickens is healthier.

[0035] Step Eleven: Using the same steps as Steps One, Two, Three, and Four, and proceeding in parallel with Steps Five, Six, Seven, Eight, and Nine, prepare clean manure trays (80cm*80cm) at 6 PM the day before, at 1 day, 7 days, 14 days, 28 days, 35 days, and 42 days of age. Place these trays under the selected empty cages for each group. Line the manure trays with 80cm*80cm filter paper to ensure cleanliness, and label and record the trays. At the same time, select one chicken of the same size (with minimal weight difference) from each repeat in each group and keep it in an empty cage for manure observation.

[0036] Step 12: For each group and each replicate, the fecal area was measured and statistically analyzed at 6:00 AM on the second day. The procedure was as follows: the filter paper for each replicate was taken out at the same time, then placed in the air to dry for 1 hour, and the x-axis and y-axis were marked with a ruler. The diffusion area was then measured and statistically analyzed, and the fecal weight was also statistically analyzed. The results were then summarized.

[0037] Step Thirteen: In the statistical experiment, compare the sum of the areas of water diffusion in the feces of each chicken in each replicate with the percentage of the total weight of the feces of that chicken. This comparison can be made horizontally or vertically.

[0038] This method provides a direct and convenient way to determine that, compared to other groups, compound organic acids are the most effective for the gut health of Sanhuang chickens with long-term use. It can serve as a reference for farms when selecting feed additives. Furthermore, this method can derive a trend line equation, allowing for the assessment of the overall gut health of the flock under specific feeding conditions at this stage. It can also predict the impact of this feed (control group) on the chicken's gut, providing a reference for feeding interventions and serving as a method for future livestock artificial intelligence, analog-to-digital conversion, and raw data identification and collection. This method is objective, long-term, systematic, and easily replicable. Attached Figure Description

[0039] Figure 1 This is an overall flowchart of a method for determining the health of a chicken flock proposed in this invention.

[0040] Figure 2 This is a graph showing the number of experimental records for a method for determining the health of a chicken flock proposed in this invention.

[0041] Figure 3 This is a percentage chart showing the experimental records of a method for determining the health of a chicken flock proposed in this invention.

[0042] Figure 4 This is a comparative experimental record of a method for determining the health of a chicken flock proposed in this invention. Detailed Implementation

[0043] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments.

[0044] Reference Figure 1-4 A method for determining the health of a flock of chickens, specifically including the following steps:

[0045] (1) Experimental chicks: One-day-old Sanhuang broiler chicks of the same breed, with uniform plumage and high health were selected. The main indicators were that the weight uniformity of the newly hatched chicks was ≥85%. The body temperature of the chicks was measured (the temperature difference was less than 0.3 degrees Celsius, and the chicks were active and uniform). The chicks showed no signs of dehydration, their abdomens were well healed, and they felt strong when held. The difference between shank length and body length was small. They were uniform in size, had bright eyes, were responsive, had loud calls, and were lively and active.

[0046] (2) The parent stock of the experimental chicks: high uniformity, thorough eradication of pullorum disease and fowl leukemia, ensuring that commercial broiler chicks are free from vertically transmitted diseases;

[0047] (3) Temperature: The temperature of the brooding room is 33-35℃ in the first week, and then decreases by 2-3℃ each week until the room temperature is maintained at 21℃.

[0048] (4) Farming mode: H-type cage rearing;

[0049] (4) Humidity: 60%-70% for 1-10 day old, 50%-60% for after 10 day old;

[0050] (5) Ventilation: Open chicken houses rely on natural ventilation, mainly through opening and closing windows and ventilation holes;

[0051] For enclosed chicken houses: Calculate the ventilation volume based on the house volume, ventilation coefficient, and number of chickens, and determine the opening of the air inlets. Use transverse ventilation, longitudinal ventilation, or transitional ventilation, depending on the age of the chickens.

[0052] (6) Lighting: 24-hour lighting for 1-2 day old chickens, followed by 23-hour lighting and 1 hour of darkness to help them adapt to the dark environment and prevent overcrowding and death in the event of a power outage. Light intensity: around 2.7 watts / m2 for 1-3 weeks, then 0.7-1.2 watts / m2 after 4 weeks, with a lamp height of 1.8-2m.

[0053] (7) Density: Week 1: 50-60 birds / m² 2 Week 2: 40 birds / m 2 Week 3: 30 birds / m 2 Week 4: 25 birds / m 2 Week 5: 20 birds / m 2 Week 6: 15 birds / m 2 ;

[0054] (8) Environment: Ensure that the chicken coop is disinfected once a week;

[0055] (9) Drinking water: Water supply via water line. Alternatively, water can be supplied via kettle for the first 3 days, followed by water supply via water line. When supplying water via water line, ensure the water pressure is appropriate for the child's age.

[0056] (10) Diet: The basal diet is the same for different groups. Early stage (1-21 days old) chick feed and late stage (22-42 days old) growing chicken feed;

[0057] (11) Immunity:

[0058] One dose of Malik vaccine was administered subcutaneously into the neck of a 1-day-old bird (at the hatchery).

[0059] 7-day-old infants receive Newcastle disease bivalent vaccine via drinking water immunization

[0060] One dose of infectious bursal disease vaccine for 12-day-old birds, administered orally.

[0061] 0.3 ml of H5N1 influenza oil vaccine per animal, administered subcutaneously.

[0062] 21-day-old clone 30 or Newcastle disease four-line + infectious bronchitis bivalent vaccine, 2 times the dose, nasal drops, eye drops.

[0063] 0.5 ml of new bronchogenic vaccine per animal, administered subcutaneously or intramuscularly.

[0064] 28-day-old infants receiving double the dose of immunization drinking water for their bursal disease

[0065] Two doses of Newcastle disease I strain 50-day-old infants were administered intramuscularly.

[0066] (12) Feeding schedule: Increase according to the standard as the child grows.

[0067] The following is the SS flock identification method. The standard flock unit is 600 birds, aged 1-42 days. The sample size can be increased according to the actual production situation.

[0068] Quantitative function curve method

[0069] Method description:

[0070] (1) At 18:00 the day before, on days 1, 7, 14, 28, 35 and 42, prepare clean manure trays (80cm×80cm) and place them under the cages of each group and each set. Line the manure trays with plastic film to ensure cleanliness, and number and record them. (The manure trays should be marked with lines and numbers using an oil-based marker, and then the plastic film should be tightly attached to the manure trays, or lines and numbers can be drawn directly on white paper and then tightly attached to the manure trays).

[0071] (2) The feces trays will be moved uniformly 12 hours after the start, and then the feces in the trays will be counted. The rule is to count from top to bottom and from left to right, counting the number of colored feces, the number of loose feces, and the total number of feces, and then summarizing the results. For example, if there are 5 loose feces, count the number "5".

[0072] (3) After the data is collected, remove the feces and clean the manure trays, while the chickens continue to be fed according to the normal feeding procedure.

[0073] (4) After all 7 6-week periods were statistically analyzed, the data were organized, and each group corresponds to a sparse function curve.

[0074] (5) By comparing the trends of the function curves (trend lines), the equation of the trend line can also be obtained to determine which group of chickens is healthier.

[0075] Explanation:

[0076] (1) Control group: fed with basal diet; Experiment 1: basal diet + 0.2g / kg licorice extract; Experiment 2: basal diet + 0.2g / kg Eucommia ulmoides extract; Experiment 3: basal diet + 2g / kg crude extract of Codonopsis pilosula and Astragalus membranaceus; Experiment 4: basal diet + 1.5g / kg compound organic acid.

[0077] (2) Figure 4 As shown, the results of the five experimental groups were statistically analyzed and represented by linear trend lines. It was found that, overall, the intestinal health of the entire flock gradually deteriorated, possibly due to increasing age and cellular aging. However, the trend lines directly indicate that the compound organic acid group showed the best trend in fecal incontinence rate, outperforming the licorice extract group, the eucommia extract group, and the crude extracts of codonopsis and astragalus from natural plants.

[0078] (3) Figure 4 For example, the linear trend line equations for the control group, licorice extract group, eucommia extract group, crude extracts of natural plants Codonopsis pilosula and Astragalus membranaceus group, and compound organic acid group are respectively: y = 0.009x + 0.0173; y = 0.0071x + 0.019; y = 0.0062x + 0.0236;

[0079] y = 0.0049x + 0.0238; y = 0.0051x + 0.0188, where x is the age in days and y is the number of loose stools. From this, we can infer the intestinal condition of the chickens at a certain age under these feeding conditions, i.e., the model of the chicken flock under these experimental conditions, and thus determine the overall intestinal health of the flock. Alternatively, the value of k in y = kx + b (k ≠ 0) can be used to determine whether the intestinal condition of each group is improving or deteriorating compared to the initial stage. If k > 0, it indicates a deterioration compared to the baseline age; if k < 0, it indicates an improvement compared to the baseline age.

[0080] (4) For longer experimental periods, integrals are more accurate. For example, to accurately compare the probability of rare feces from 1 day to 14 days old, integrating the piecewise trend line and summing the results is more accurate and intuitive. For shorter experimental periods, a linear function trend can be used for comparison.

[0081] Cumulative area mean method:

[0082] Method description:

[0083] (1) Select chicks with small weight differences and an initial weight of 27±1 grams at standard weight. Adjust the cage density to ensure that the stocking density of all groups is the same. Conduct fecal observation experiments at 7, 14, 28, 35, and 42 days of age. For example: In this experiment (which can be conducted in parallel with the quantitative function curve method), a total of 600 chickens were used in five groups, with each group divided into 6 replicates and 20 chickens per replicate.

[0084] (2) At 6 p.m. the day before the 7th, 14th, 28th, 35th and 42nd day of age, one chicken of the same size, weight and health status was selected for each replicate and placed in a separate cage for the experiment.

[0085] (3) At 6:00 AM on days 7, 14, 28, 35, and 42, place white paper of the same quality, specifications, and absorbency under the cage in a tray. Simultaneously add the same quality and weight of feed. Under the same conditions, observe and measure the feces after 4 hours, counting the samples. Use high-precision calipers for measurement. After measurement, weigh the feces.

[0086] (4) By comparing the sum of the areas of water diffusion in the feces of each chicken in each replicate with the percentage of the weight of the feces of that chicken, the comparison can be made at 7 days, 14 days, 28 days and 42 days of age, and then the horizontal trend of the change can be compared (similar to the quantitative function curve method). The diffusion ratio of the five additives can also be compared vertically to judge the quality of the additives and the intestinal condition of the chickens.

[0087] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.

Claims

1. A method for determining the health of a chicken flock, characterized in that, Specifically, the following steps are included: Step 1: Experimental chicks: Select 1-day-old Sanhuang broiler chicks of the same breed, with uniform plumage and high health. The main indicator is that the weight uniformity of newly hatched broiler chicks is ≥85%. Step 2: Test chick parent stock: high uniformity, thorough eradication of pullorum disease and fowl leukosis, ensuring that commercial broiler chicks are free from vertically transmitted diseases; Step 3: Carry out feeding and disease prevention according to the predetermined experiment; Step 4: The experiment was divided into groups A, B, C, D and E. The number of chickens was the same and each group had at least 3 replicates. Each replicate was cage-housed. This grouping principle is consistent with the arbitrary feeding experiment. Step 5: Different additives were selected in the experiment, but the same addition method was used. This group selected licorice extract, eucommia extract, fermented natural plant complex, and compound organic acid to verify the effects of these four substances on the health of the chicken flock. A means no feeding of the above substances, and B, C, D, and E are the four substances mentioned above. Step Six: At 6 PM the day before, prepare clean manure trays (80cm*80cm) for each group of babies at 1 day, 7 days, 14 days, 28 days, 35 days, and 42 days of age. Place the trays under the cages of each group and each set. Line the trays with ordinary graph paper (80cm*80cm) and attach a transparent plastic film tightly to the paper to ensure cleanliness. Label the trays and record the contents. Step 7: At 6:00 AM on the second day, count the feces in the feces tray, following the principle of counting from top to bottom and from left to right. Count the number of colored feces, the number of loose feces, and the total number of feces, and then summarize the results. Step 8: After the count is completed at 6:00 AM, the feces are removed, and a new liner is laid down. The count is repeated at 6:00 PM. Similarly, the count is carried out on the designated dates of the experiment according to the plan. Step 9: After counting all 6 times, organize the data, and each group corresponds to a sparse function curve; Step 10: By comparing the slopes of the function trend lines, we can derive the equation relating the percentage to the age of the chickens, which helps explain which flock is healthier. Step Eleven: Using the same steps as Steps One, Two, Three, and Four, and proceeding in parallel with Steps Five, Six, Seven, Eight, and Nine, prepare clean manure trays (80cm*80cm) at 6 PM the day before, at 1 day, 7 days, 14 days, 28 days, 35 days, and 42 days of age. Place these trays under the selected empty cages for each group. Line the manure trays with 80cm*80cm filter paper to ensure cleanliness, and label and record the trays. At the same time, select one chicken of similar size (with minimal weight difference) from each repeat of each group and confine it in an empty cage for manure observation. Step 12: For each group and each repetition, the fecal area was measured and statistically analyzed at 6:00 AM on the second day. The procedure is as follows: each filter paper is removed at the same time, then placed in the air to dry for 1 hour, and the x-axis and y-axis are marked with a ruler. Then the diffusion area is measured and the fecal weight is counted, and the results are summarized. Step Thirteen: In the statistical experiment, compare the sum of the areas of water diffusion in the feces of each chicken in each replicate with the percentage of the total weight of the feces of that chicken, and make both horizontal and vertical comparisons.

2. The method for determining the health of a chicken flock according to claim 1, characterized in that, The selection of chicken flocks in step one includes the following conditions: using healthy 1-day-old Sanhuang chickens or any experimental flocks; the ventilation volume used in the experiment is the minimum ventilation volume in the early stage (1-21 days old) and a moderately variable ventilation volume in the later stage (22-42 days old); the chicken house is a conventional closed chicken house; the lighting is adjusted according to the age of the chickens; the feed is the early stage (1-21 days old) feed and the later stage (22-42 days old) feed; the drinking water is conventional drinking water and the water pressure is controlled according to the age of the chickens; the feeding program is increased according to the standard as the age of the chickens.

3. The method for determining the health of a chicken flock according to claim 1, characterized in that, The fermented natural plant complex is a crude extract of Codonopsis pilosula and Astragalus membranaceus, and the complex organic acids are a hexacarboxylic acid consisting of formic acid, propionic acid, phosphoric acid, lactic acid, benzoic acid, and citric acid.

4. The method for determining the health of a chicken flock according to claim 1, characterized in that, In step two, the positive rate of avian leukosis in parent breeder chickens was 0%, the positive rate of pullorum disease was ≤5%, and the positive rate of pullorum disease in commercial breeder chickens was ≤10%, while the positive rate of avian leukosis was 0%.

5. The method for determining the health of a chicken flock according to claim 1, characterized in that, The feeding and disease prevention in step three specifically includes the following steps: During feeding, the indoor temperature should be maintained at 33-35℃ for the first week, decreasing by 2-3℃ each week thereafter until it reaches 21℃. Observe the chickens' spirit, appetite, and droppings during morning and evening feedings. Ensure the chicken house is well-ventilated to maintain fresh air and prevent chicken poisoning from excessive ammonia concentration. Avoid contaminating feed troughs and water troughs with droppings, and regularly clean and disinfect all equipment. Disease prevention should be carried out according to the farm's immunization schedule: Marek's disease vaccine at 1 day old; Newcastle disease and infectious bronchitis bivalent vaccine in drinking water at 7 days old; infectious bursal disease vaccine orally at 12 days old, and H5N1 influenza oil vaccine subcutaneously at 12 days old; Newcastle disease four-strain and infectious bronchitis bivalent vaccine in nasal and ocular drops at 21 days old; infectious bursal disease vaccine in drinking water at 28 days old; and Newcastle disease I strain vaccine intramuscularly at 50 days old.

6. The method for determining the health of a chicken flock according to claim 1, characterized in that, Before raising chickens, the chicken house needs to be disinfected, including removing chicken droppings, dust from the roof and walls, rinsing the chicken house, and then carrying out chemical disinfection. Use disinfectant to rinse feed buckets and water bottles, then soak them in disinfectant water for 12-24 hours, spray the entire chicken house with disinfectant, and use formaldehyde and potassium permanganate diluted in 2:1 water for fumigation disinfection.

7. The method for determining the health of a chicken flock according to claim 1, characterized in that, The cage rearing mainly involves 3-layer H-cage rearing, with manual manure removal using manure trays.

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