Emotional state and neuroelectrophysiological state regulating device, verification system and method

Abstract

The present invention discloses an emotional state and neuroelectrophysiological state regulating device, verification system and method, which belongs to the field of physical state regulation, and includes: a voltage generating module, which is used to output an extremely low frequency high voltage; a radiation module, which is used to convert the high voltage into an electric field, so that the subject is in the electric field, and then regulates the subject's subjective emotions, physiological reactions, neural electrical activities, etc. After the electric field intervention, the subject showed a lower level of cerebral cortex arousal, which was manifested as an increase in the power spectrum density of α and θ frequencies, and a significant decrease in subjective emotions related to exploration motivation and alertness, indicating that the subject was in a more relaxed state after acute stress; the extremely low frequency high voltage electric field can promote emotional regulation, and the overall negative emotion score of the subjects after the intervention is lower, and the anxiety level is also significantly reduced. The heart rate and blood pressure levels of the subjects after the electric field intervention are reduced, and both subjective and physiological indicators reflect that the physical and mental state of the subjects after the electric field intervention is relatively more stable.

Description

Technical Field

[0001] The present invention relates to the field of body state regulation, and in particular to an emotional state and neuro-electrophysiological state regulation device, a verification system and a method. Background Art

[0002] Competition in modern society is fierce, and people are facing various social pressures, which have different effects on people's subjective emotions, neural electrical activity and other physical conditions. Some people use drugs to relieve the pressure they face. At present, people pay more and more attention to "green consumption", and they pursue "green therapy" for the regulation of physical condition. This is because people have realized that excessive use of drugs and the toxic and side effects of drugs are harmful to human health. In addition, with the advent of an aging society in my country, the medical model is constantly transforming from the traditional diagnosis and treatment model to the early detection and prevention of diseases, that is, the health management model. People are no longer relying solely on medication for regulation, and they are eagerly looking forward to the emergence of "green therapy". With the popularization of the value of "green therapy", people are more concerned about non-invasive physical means that can achieve physical state regulation without harming the body, pain, or toxic side effects. Summary of the invention

[0003] The purpose of the present invention is to overcome the problems of the prior art and to provide an emotional state and neuro-electrophysiological state regulating device, a verification system and a method.

[0004] The object of the present invention is to achieve the following technical solution: an emotional state and neuroelectrophysiological state regulating device, the device comprising:

[0005] Voltage generation module, used to output 50Hz-60Hz, 0KV-30KV high voltage;

[0006] The radiation module is used to convert high voltage into an electric field, so that the subject is placed in the electric field, thereby regulating the subject's physical state, including subjective emotions, physiological reactions, and neural electrical activities.

[0007] In one example, the radiation module includes a head electrode and a foot electrode, both of which are fixed on a carrier carrying the subject, the head electrode is located above the subject's head, and the foot electrode is located below the carrier;

[0008] An insulating material layer is provided between the test subject's feet and the foot electrodes, the foot electrodes are connected to the output end of the voltage generating module, and the head electrodes are grounded.

[0009] It should be further explained that the technical features corresponding to the above-mentioned device examples can be combined or replaced with each other to form a new technical solution.

[0010] The present invention also includes a verification system for an emotional state and a neuroelectrophysiological state regulating device, the system including the device formed by any one of the above examples or a combination of two examples, and further including:

[0011] The information collection module is used to collect the subject's biological information, including state anxiety information, emotional information, blood pressure information, heart rate information, and EEG information;

[0012] The stress induction module is used to apply pressure to the subject to induce a stress response in the subject.

[0013] In one example, the stress induction module is one or more of a Trier social stress test submodule, a social assessment cold pressor submodule, and a Maastricht acute stress submodule.

[0014] In one example, the system also includes a point detection task module for applying two visual stimuli in a left-right structure or an up-down structure to the subject, and after the visual stimulus disappears, allowing the subject to identify the position or direction of the detection point that appears.

[0015] It should be further explained that the technical features corresponding to the above system examples can be combined or replaced with each other to form a new technical solution.

[0016] The present invention also includes a method for verifying an emotional state and a neuroelectrophysiological state regulating device, which is implemented based on the system formed by any one of the above examples or a combination of two examples, and includes the following steps:

[0017] The first biological information of the subjects is collected through the information collection module, including state anxiety information, emotional information, blood pressure information, heart rate information, and EEG information;

[0018] After applying stress to the subjects through the stress induction module, the subjects were subjected to a second bio-information collection, including state anxiety information, emotional information, blood pressure information, heart rate information, and resting EEG information;

[0019] The voltage generation module is turned on to place the subject in an electric field to adjust the subject's physical state. When the adjustment is completed, the third biological information collection is performed on the subjects in the experimental group. At the same time, the voltage generation module is turned off. After the adjustment lasts for the same period of time as the physical state adjustment, the third biological information collection is performed on the subjects in the control group, including state anxiety information, emotional information, blood pressure information, and heart rate information.

[0020] The verification results were obtained based on the biological information collected three times.

[0021] In one example, the method includes the following steps:

[0022] The first biological information of the subjects is collected through the information collection module, including state anxiety information, emotional information, blood pressure information, heart rate information, and EEG information;

[0023] After applying stress to the subjects through the stress induction module, the subjects were subjected to a second bio-information collection, including state anxiety information, emotional information, blood pressure information, heart rate information, and resting EEG information;

[0024] The voltage generation module is turned on to place the subject in an electric field to adjust the subject's physical state. When the adjustment is completed, the third biological information collection is performed on the subjects in the experimental group. At the same time, the voltage generation module is turned off. After the adjustment lasts for the same period of time as the physical state adjustment, the third biological information collection is performed on the subjects in the control group, including state anxiety information, emotional information, blood pressure information, and heart rate information.

[0025] Threat stimuli were applied to the subjects through the point detection task module, and the subjects were asked to perform the corresponding tasks. The reaction time and reaction accuracy of the subjects in the experimental group and the control group were recorded. Finally, the fourth bio-information collection was conducted on the subjects in the experimental group and the control group, including state anxiety information, emotion information, blood pressure information, and heart rate information;

[0026] The verification results were obtained based on the biological information collected four times.

[0027] Compared with the prior art, the present invention has the following beneficial effects:

[0028] The subjects were placed in an extremely low frequency (50Hz-60Hz) high voltage (0KV-9KV) electric field. After the electric field intervention, the subjects showed lower levels of cerebral cortical arousal, manifested as enhanced alpha and theta activities (increased power spectral density of alpha and theta frequencies), and a significant reduction in subjective emotions related to exploratory motivation and alertness, indicating that the subjects were in a more relaxed state after acute stress; in addition, the extremely low frequency and high voltage electric field can promote emotional regulation. After the intervention, the subjects had lower overall negative emotion scores and significantly reduced anxiety levels. At the same time, the heart rate and blood pressure levels of the subjects after the electric field intervention decreased. Both subjective and physiological indicators reflected that the physical and mental state of the subjects after the electric field intervention was relatively more stable. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] The specific implementation methods of the present invention are further described in detail below in conjunction with the accompanying drawings. The accompanying drawings described herein are used to provide a further understanding of the present application and constitute a part of the present application. The same reference numerals are used in these drawings to represent the same or similar parts. The schematic embodiments of the present application and their descriptions are used to explain the present application and do not constitute improper limitations on the present application.

[0030] Figure 1 A device block diagram provided for an example of the present invention;

[0031] Figure 2 A method flow chart provided for an example of the present invention;

[0032] Figure 3 A method flow chart is provided for a preferred example of the present invention. DETAILED DESCRIPTION

[0033] The technical solution of the present invention is described clearly and completely below in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0034] In the description of the present invention, it should be noted that the directions or positional relationships indicated by "center", "up", "down", "left", "right", "vertical", "horizontal", "inside", "outside", etc. are directions or positional relationships based on the drawings, which are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention. In addition, the use of ordinal numbers (e.g., "first and second", "first to fourth", etc.) is to distinguish objects, and is not limited to this order, and cannot be understood as indicating or implying relative importance.

[0035] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, "installation", "connection" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0036] In addition, the technical features involved in the different embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.

[0037] In one example, if Figure 1As shown, an emotional state and neuroelectrophysiological state regulating device includes a voltage generating module and a radiation module. The voltage generating module is used to output a 50Hz-60Hz, 0KV-9KV high voltage voltage, and can be optionally an EF generator. Optionally, the voltage generating module includes a frequency modulation and voltage regulating submodule and a boosting submodule. The frequency modulation and voltage regulating submodule is used to adjust the voltage and frequency of the input power supply to obtain the input voltage required by the boosting module; the boosting module is used to boost the input voltage and output the 50Hz-60Hz, 0KV-9KV high voltage voltage required by the electric field.

[0038] The radiation module is used to convert high voltage into an electric field, so that the subject is placed in the electric field, thereby regulating the subject's physical state, including subjective emotions, physiological reactions, and neural electrical activities.

[0039] Optionally, the radiation module includes a head electrode and a foot electrode, both of which are fixed on a carrier carrying the subject, the head electrode is located above the subject's head, and the foot electrode is located below the carrier; an insulating material layer is provided between the subject's feet and the foot electrodes, the foot electrodes are connected to the output end of the voltage generating module, and the head electrode is grounded. Optionally, the carrier can be a chair, a stool, etc.

[0040] In this example, during the electric field (EF) exposure, the subject sat on a chair connected to an EF generator (XHGM-30), and two electrodes were fixed to the chair: one was located above the head (called the head electrode), and the other was located below the chair (called the foot electrode). A plastic plate was inserted between the foot and the electrode to ensure that the electrode did not directly contact the subject's body, and a closed electric field was formed between the two electrodes. The EF generator applied a 50Hz electric potential with a root mean square amplitude of 30kV to the foot electrode, and the head electrode served as the ground for the charge. After the electric field intervention, the subject's biological information, including state anxiety information, emotional information, blood pressure information, heart rate information, and EEG information, was collected, and then it was concluded that the 50Hz high-voltage electric field had different effects on subjective emotions, physiological reactions, and neural electrical activities, but to some extent reduced the alertness level of all aspects of the body and mind, that is, the electric field generated by the emotional state and neuroelectrophysiological state regulating device of the present invention can change the emotional state by reducing the arousal level of the cerebral cortex (brain activity level) to reduce people's anxiety, stress, etc., providing a reference direction for the current research of non-invasive treatment equipment.

[0041] The present invention also includes a verification system for an emotional state and a neuroelectrophysiological state regulating device, the system including an emotional state and a neuroelectrophysiological state regulating device formed by any one of the above examples or a combination of multiple examples, and further including:

[0042] The information collection module is used to collect the subject's biological information, including state anxiety information, emotional information, blood pressure information, heart rate information, and EEG information; optionally, the biological information also includes saliva samples.

[0043] The stress induction module is used to apply pressure to the subject to induce a stress response in the subject.

[0044] Specifically, the information collection module includes a state anxiety information collection submodule, an emotion information collection submodule, a blood pressure collection submodule, a heart rate collection submodule and an EEG collection submodule. Among them, the state anxiety information collection submodule stores the "State-Trait Anxiety Inventory", and collects the state anxiety information of the subject based on the "State-Trait Anxiety Inventory" (STAI); the emotion information collection submodule stores the "Positive and Negative Emotion Scale-Extended Version", and collects the emotion information of the subject based on the "Positive and Negative Emotion Scale-Extended Version" (PANAS-X). The blood pressure collection submodule can be a sphygmomanometer, which is used to collect the blood pressure information of the subject; the heart rate collection submodule can be a heart rate meter, which is used to collect the heart rate information of the subject; the EEG information collection submodule can be an EEG collection device, and then collect the EEG signals of the subject. Optionally, the EEG of the subjects was collected by an electrode cap with 32 scalp electrodes (Fp1, Fp2, F7, F3, Fz, F4, F8, FT7, FC3, FCz, FC4, FT8, T7, C3, Cz, C4, T8, TP7, CP3, CPz, CP4, TP8, P7, P3, Pz, P4, P8, O1, Oz, O2, M1 and M2), and the electrodes on the electrode cap were placed according to the international standard position (10-20 system (Klem et al., 1999)). Each electrode was referenced to the mean of the left side and mastoid (M1 and M2, respectively), the ground electrode was located in front of Fz, the impedance was kept below 10 kΩ, and the data were acquired at a sampling rate of 512 Hz.

[0045] Optionally, the stress induction module is one or more of the Trier social stress test submodule, the social evaluation cold pressor submodule, and the Maastricht acute stress submodule. In this example, the Maastricht acute stress submodule is preferably used, and the acute stress induction is realized based on the Maastricht Acute Stress Test (MAST) technology. The Maastricht acute stress submodule includes a control module, a display connected to the control module, and a voice acquisition and playback device. MAST includes a preparation phase lasting 5 minutes and a stress phase lasting 10 minutes, during which physiological and psychosocial stress is applied. During the stress phase, the subject's hand is immersed in cold water (0-4°C), and the duration is controlled by the experimenter and does not exceed 90 seconds. After the immersion is completed, the display is used to display the questions so that the subject immediately starts a mental arithmetic task, such as constantly subtracting 17 from 1,023, and the answer of the subject is collected and played by the voice acquisition and playback device. In addition, the subject is required to complete the task as quickly and accurately as possible within at least 45 seconds. Throughout the task, the experimenter closely observed the subjects and provided negative feedback via a voice capture and playback device and a monitor when errors occurred. The subjects were informed that their facial reactions would be recorded for further analysis (increasing social evaluation pressure, but not actually recorded). The test alternated between hand immersion tests (HITs) and mental arithmetic tasks (MATs).

[0046] In one example, the system further includes a dot detection task module, which is used to apply two visual stimuli in a left-right structure or an up-down structure to the subject, and after the visual stimulus disappears, the subject is made to identify the position or direction of the detection point that appears subsequently; wherein the visual stimuli include mixed pairs (threat picture-neutral picture / neutral picture-threat picture), neutral pairs (neutral pictures-neutral pictures) and threat pairs (threat picture-threat picture). In this example, the dot detection task module includes a control module, a display connected to the control module, and a button. The stimuli of the dot detection task are presented on a 14-inch black background display (resolution of 1024×768, refresh rate of 60Hz). The threat stimuli in this example are selected from the Geneva Affective Picture Database (GAPED) (Dan-Glauser & Scherer, 2011), including neutral and fear pictures with 640×480 dots per inch (dpi). The experiment includes 140 pictures, of which 70 are fearful and 70 are neutral. In each trial, the “+” dot was first displayed in the center of the screen for 750 milliseconds, and then a set of randomly presented threat and neutral picture pairs were presented within 600 milliseconds. These threat and neutral picture pairs included three types of pairs: mixed pairs, neutral pairs, and threat pairs. They were counterbalanced so that each picture could appear in each position an equal number of times. Then, the target stimulus was randomly presented for 500 milliseconds to the left or right of the fixation point. It was a vertically centered dot with a size of 1cm×1cm. After the interface of the target stimulus disappeared, the subject had to press the F or J key within 1,000 milliseconds to judge the position of the dot (left or right) to ensure the correct rate. The key position was counterbalanced between subjects. Failure to respond within 1,000 milliseconds was regarded as a response error, and the subject automatically entered the next trial. For mixed pairs, 200, 40, and 40 trials were arranged, respectively, for a total of 280 times. In the 200 trials of the mixed pairs, 100 trials were when the target appeared at the location where the threat picture disappeared (congruent condition), and the other 100 trials were when the target appeared at the location where the neutral picture disappeared (incongruent condition). The subjects' eyes were located at the center of the screen at a distance of approximately 50 cm. E-prime 2.0 software was used to present stimuli and collect data.

[0047] The present invention also includes a verification method for an emotional state and a neuroelectrophysiological state regulating device, which is implemented based on a verification system formed by any one or more of the above examples, such as Figure 2 As shown, the method comprises the following steps:

[0048] S01: The subject's first bio-information is collected through the information collection module, including state anxiety information, emotional information, blood pressure information, heart rate information, and EEG information. The first collected bio-information is used as the baseline for subsequent bio-information comparison (T0); the subject preferably arrives at the laboratory, reads and signs the informed consent form in a separate room, and then rests for 5 minutes before starting step S01. At the beginning of the neurophysiological measurement, at the beginning of the experiment, the subject records the resting state EEG activity (RS-EEG) for 5 minutes with eyes open as the baseline measurement of brain activity.

[0049] S02: After applying stress to the subjects through the stress induction module, that is, completing the "Maastricht Acute Stress Test" (MAST; 15 minutes), the subjects were subjected to a second bio-information collection, including state anxiety information, emotional information, blood pressure information, heart rate information, and resting EEG information. Specifically for neurophysiological measurements, RS-EEG was collected 5 minutes after stress induction.

[0050] S03: The subjects were randomly assigned to the EF group (high-voltage electric field exposure group, corresponding to the experimental group) or the Sham group (false exposure group, corresponding to the control group), and EEG data were recorded simultaneously (20 minutes); at the end of the exposure, the third biological information collection was carried out, including state anxiety information, emotional information, blood pressure information, and heart rate information;

[0051] S04: The verification result is obtained based on the biological information collected three times.

[0052] To complete the above validation method, 40 healthy college students aged between 20 and 26 were recruited through an online questionnaire. Eligibility criteria were as follows: a) subjects were between 18 and 29 years old; b) subjects held a bachelor's degree or above. Exclusion criteria were as follows: 1) subjects had chronic diseases such as hypertension and cardiovascular disease; 2) subjects had acute illness or exacerbation of chronic diseases in the past two weeks; 3) subjects had psychiatric, neurological or endocrine diseases; 4) subjects had oral diseases; 5) subjects used medications that may affect hormone levels; 6) subjects were dependent on or abused substances / alcohol; 7) subjects smoked; 8) subjects showed irregular circadian rhythms; 9) subjects had metal instruments implanted in their bodies; or 10) subjects participated in experiments that required saliva collection.

[0053] For the experimental setting of this validation method, subjects were randomly assigned to intervention (high-voltage electric field exposure, EF group) or control (sham exposure, Sham group) conditions in a 1:1 ratio before the start of subject recruitment according to random numbers generated by a computer program. In the electric field (EF) exposure condition, subjects sat in a custom-made chair with an operating electric field system for 20 minutes. In the sham exposure condition, subjects sat in the same chair for 20 minutes, but the EF generator was not activated. Subjects in the EF group were exposed to EF generated by applying 50Hz and 9kV through foot electrodes and head electrodes. To ensure that the subjects were unaware of the exposure protocol, the operating module of the electric field generator was covered, and all subjects were told that the EF generator was running. The study was conducted in an independent, quiet and distraction-free laboratory. During the experiment, the air conditioner in the room was running at a constant temperature.

[0054] Further, the three collected EEG information was processed, and the verification results were obtained based on the biological information collected three times. Specifically, the Butterworth filter notch filtering was used to remove 50Hz interference, and then a 0.5-30Hz bandpass filter was performed. The original EEG signal was processed using the MATLAB-based LETSWAVE data processing software package. This involves re-referencing, removing unnecessary electrodes (VEOG, HEOG, TRIGGER), Butterworth notch filtering to eliminate 50Hz power line interference, and then a 0.5-30Hz bandpass filter. Then, independent component analysis (ICA) was used to remove artifacts including eye movements and blinks, and 1-2 independent components were usually removed for each subject. After that, the data was divided into 10-second periods and baseline correction was performed throughout the period. Trials with amplitudes exceeding 100 (positive or negative) were excluded.

[0055] Further, the statistical analysis of the bioinformation collected three times was performed by the Statistical Package for Social Sciences (SPSS, version 23.0; ). The normality of the data was checked using quantile-quantile (QQ) plots and Shapiro-Wilk test. Highly skewed data were subjected to natural logarithm transformation. Independent sample t-tests and Mann-Whitney U tests were used to determine the differences between the groups in demographic factors and baseline measurements. ANCOVA model analysis was used to compare the changes in physiological and psychological indicators between the two groups over time, taking into account the factors of condition (two levels: EF and Sham exposure) and measurement time point (second and third bioinformation collection). The baseline (T0) results were used as a covariate to try to control the known and potential effects of baseline imbalance on subsequent results. The preprocessed EEG signals were subjected to fast Fourier transform (FFT) to determine the power of each spectrum, including delta wave (0.5-4Hz), theta wave (4-8Hz), and alpha wave (8-13Hz). Repeated measures ANOVA was used to examine the differences in the spectrum under different conditions. In the dot-probe task, the behavioral data of each subject were subjected to the following data manipulation: (1) trials with incorrect responses were deleted; (2) trials with response durations between 200 ms and 1,000 ms were deleted; and (3) trials with response durations exceeding three standard deviations were deleted. After calculating the attentional orientation and attentional disengagement scores for the subjects included in the analysis, a 2 (group: intervention / control) × 2 (attentional bias: attentional orientation / attentional disengagement) repeated-measures ANOVA was conducted. Attentional orientation was the mean reaction time in the inattentional allocation condition minus the mean reaction time in the congruent condition. Attentional disengagement was the mean reaction time in the incongruent condition minus the mean reaction time in the inattentional allocation condition. Subsequent Bonferroni tests were used for multiple comparisons. When the spherical shape requirement was not met, Greenhouse–Geisser-corrected p values ​​were reported. A two-sided alpha of 0.5 was set for all analyses. The measured effect sizes were expressed as partial Eta squares (ηp2>0.01, 0.06, and 0.14 indicated small, medium, and large effects, respectively). Data beyond three standard deviations, whether positive or negative, were removed.

[0056] Optionally, in another preferred example, the verification method further introduces a point detection task module to apply threat stimulation to the subject, and the other steps are the same as the verification method of the previous example, such as Figure 3 As shown, at this time, the verification method includes the following steps:

[0057] S11: collecting the subject's first biological information through the information collection module, including state anxiety information, emotional information, blood pressure information, heart rate information, and EEG information;

[0058] S12: After applying stress to the subject through the stress induction module, the subject's bio-information is collected for the second time, including state anxiety information, emotional information, blood pressure information, heart rate information, and resting EEG information;

[0059] S13: The subjects were randomly assigned to the EF group or the Sham group, and EEG data were recorded simultaneously (20 minutes); at the end of the exposure, the third biological information collection was performed, including state anxiety information, mood information, blood pressure information, and heart rate information;

[0060] S14: applying threat stimulation to the subjects through the point detection task module, and asking the subjects to perform the corresponding task (15 minutes), recording the reaction time and reaction accuracy of the subjects in the experimental group and the control group, and finally collecting the fourth bio-information of the subjects in the experimental group and the control group, including state anxiety information, emotion information, blood pressure information, and heart rate information;

[0061] S15: A verification result is obtained based on the biological information collected four times.

[0062] Based on the above experiments, the following conclusions were drawn: 50Hz high-voltage EF had different effects on subjective emotions, physiological reactions, and neural electrical activity, but to some extent reduced the alertness and arousal levels of all aspects of the body and mind, showing faster recovery and stronger physical and mental regulation after stress. In addition, the subjects had lower autonomic nervous system activation after experiencing stress again in a short period of time, indicating that the regulatory effect of EF has a certain persistence.

[0063] After step S12 stress, blood pressure (systolic pressure, diastolic pressure) and state anxiety level increased significantly. 30 minutes after acute stress, cortisol concentration increased significantly, indicating that MAST stress is effective.

[0064] Specifically, the experimental group showed a decrease in confidence and surprise after EF exposure: the confidence score of the experimental group decreased significantly (F(2,36)=7.65, p=0.001) and was lower than that of the control group (F(1,37)=5.23, p=0.028); the surprise of the experimental group decreased significantly (F(2,33)=5.06, p=0.009); only the EF group had a significant decrease in negative emotions after stress (F(2,36)=4.03, p=0.031). Confidence is related to the dopamine system and the desire to pursue rewards. One of the main functions of the dopamine system is to enhance exploratory behavior and generate the desire to pursue rewards (i.e., achieve goals) or seek information. Similarly, surprise is associated with alertness signals and high levels of arousal experience. Therefore, the decrease in subjects' confidence and surprise emotions may be related to reduced exploratory motivation and reduced alertness levels. This may indicate that EF exposure leads to participants having a more relaxed and safe subjective experience, which seems to be consistent with the reduction of negative emotions (NA). Although there were no significant changes in NA in both groups immediately after the stress, the EF group showed a significant decrease in NA during the subsequent experimental session compared with the control group. Lower levels of alertness may cause individuals to feel tired, sluggish, or unable to concentrate, which may reduce their confidence and reduce NA.

[0065] Compared with the electric field exposure and natural rest, systolic blood pressure decreased significantly in both groups (F(2,36)=4.57, p=0.017; F(2,36)=5.39, p=0.01), while the control group showed an increase in heart rate (F(2,35)=4.02, p=0.021). The dot-probe task using images containing threatening stimuli can be considered a stressor. The paradigm combining cognitive tasks and emotional pictures (MMST) has been validated to induce stress responses. Therefore, it is speculated that the participants may have experienced re-stress after completing the dot-probe task. Compared with EF exposure, the control group had a significant increase in heart rate after re-stress, which may reflect the re-activation of the SAM system in the control group participants, while the experimental group remained relatively stable.

[0066] During EF exposure, alpha and theta activity increased (F(2,36)=12.17, p<0.001; F(1,37)=7.33, p=0.01). Increased activity in low-frequency bands (such as alpha and theta) is generally associated with a decrease in alertness level (level of brain activity). In general, the amplitude / power of the alpha band is negatively correlated with alertness level. Existing studies have shown that theta activity is generally associated with sleep and relaxation states. In addition, increased power in alpha and theta frequencies has also been observed in various types of meditation practices. Existing studies have found that mindfulness practice can reduce physiological indicators related to stress. A study using mindfulness to improve attention showed that mindful breathing exercises not only enhanced alpha activity during training. Previous studies have shown that theta activity is generally associated with sleep and relaxation states. It is worth noting that increased theta power is associated with the use of emotion regulation strategies. Compared with experiencing emotions, active emotion regulation is associated with significant theta activation.

[0067] Based on the above results, the following hypotheses are proposed:

[0068] 1. Under stress, subjects showed lower levels of arousal after electric field intervention, as evidenced by enhanced alpha and theta activity, and a significant reduction in subjective emotions associated with exploratory motivation and alertness. Recovery is a process of psychophysiological relaxation, which is the opposite of the activation of psychophysiological systems under stress conditions.

[0069] 2. EF may promote emotion regulation, as shown by a significant reduction in negative emotions. Emotion regulation is a key ability to adapt to the environment. Emotional dysregulation is a major factor in various disorders, such as anxiety, mood, and post-traumatic stress disorder. Therefore, changes in these two aspects may contribute to rapid post-stress recovery.

[0070] 3. The effects of EF seem to last for a period of time: not only do the changes in mood last until the end of the experiment, but the final measurement of physiological indicators (such as heart rate) seems to indicate that the experimental group's response is less obvious.

[0071] The above specific implementation methods are detailed descriptions of the present invention. It cannot be determined that the specific implementation methods of the present invention are limited to these descriptions. For ordinary technicians in the technical field to which the present invention belongs, several simple deductions and substitutions can be made without departing from the concept of the present invention, which should be regarded as belonging to the protection scope of the present invention.

Claims

1. A verification system for an emotional state and a neuroelectrophysiological state regulating device, characterized in that: The system includes an emotional state and neuro-electrophysiological state regulating device, and the device includes: Voltage generation module, used to output 50Hz-60Hz, 0KV-30KV high voltage; A radiation module is used to convert high voltage into an electric field, so that the subject is placed in the electric field, thereby regulating the subject's physical state, including subjective emotions, physiological reactions, and neural electrical activities; The radiation module includes a head electrode and a foot electrode, both of which are fixed on a carrier carrying the subject, the head electrode is located above the subject's head, and the foot electrode is located below the carrier; An insulating material layer is provided between the test subject's feet and the foot electrodes, the foot electrodes are connected to the output end of the voltage generating module, and the head electrodes are grounded; The system further comprises: The information collection module is used to collect the subject's biological information, including state anxiety information, emotional information, blood pressure information, heart rate information, and EEG information; A stress induction module, used for applying pressure to the subject to induce a stress response in the subject; The stress induction module is one or more of the Trier social stress test submodule, the social assessment cold pressor submodule, and the Maastricht acute stress submodule; The system also includes a point detection task module, which is used to apply two visual stimuli in a left-right structure or a top-bottom structure to the subject, and after the visual stimulus disappears, the subject recognizes the position or direction of the detection point that appears subsequently; The system implements a verification method for an emotional state and a neuro-electrophysiological state regulating device, comprising the following steps: S11: collecting the subject's first biological information through the information collection module, including state anxiety information, emotional information, blood pressure information, heart rate information, and EEG information; S12: After applying stress to the subject through the stress induction module, the subject's bio-information is collected for the second time, including state anxiety information, emotional information, blood pressure information, heart rate information, and resting EEG information; S13: Turn on the voltage generation module to place the subject in the electric field to adjust the subject's physical state. When the adjustment is completed, collect the third biological information of the experimental group subject. At the same time, turn off the voltage generation module. After the same time as the physical state adjustment, collect the third biological information of the control group subject, including state anxiety information, emotion information, blood pressure information, and heart rate information. S14: applying threat stimulation to the subjects through the point detection task module, and making the subjects perform the corresponding tasks, recording the reaction time and reaction accuracy of the subjects in the experimental group and the control group, and finally collecting the fourth bio-information of the subjects in the experimental group and the control group, including state anxiety information, emotion information, blood pressure information, and heart rate information; S15: Verification results are obtained based on the biological information collected four times; The EEG information collected three times was processed, and then the verification results were obtained based on the biological information collected three times; the 50Hz interference was first removed using a Butterworth filter notch filter, and then a 0.5-30Hz bandpass filter was performed; the original EEG signal was processed using the MATLAB-based LETSWAVE data processing software package; then, independent component analysis was used to remove artifacts including eye movements and blinks; after that, the data was divided into 10-second periods and baseline correction was performed throughout the period; trials with amplitudes exceeding 100 were excluded; When performing statistical analysis on the biological information collected three times, the Social Science Statistical Package was used to perform statistical analysis; quantile-quantile plots and Shapiro-Wilk test were used to check the normality of the data; highly skewed data were transformed into natural logarithms; independent sample t-tests and Mann-Whitney U tests were used to determine the differences between the groups in demographic factors and baseline measurements; ANCOVA model analysis was used to compare the changes in physiological and psychological indicators between the two groups over time, taking into account the conditions and measurement time points. The conditions were EF level and Sham exposure level, and the measurement time points were the time points of the second and third biological information collections; the baseline results were used as covariates to control the effects of known and potential baseline imbalances on subsequent results; the preprocessed EEG signals were subjected to fast Fourier transform to determine the power of each spectrum, including delta waves, theta waves, and alpha waves; repeated measures analysis of variance was used to examine the differences in the spectrum under different conditions; in the dot detection task, the behavioral data of each subject underwent the following data processing: (1) removing incorrect responses The correct trials were selected; (2) trials with a response duration between 200 ms and 1,000 ms were removed; and (3) trials with a response duration exceeding three standard deviations were removed. After calculating the attention orienting and attention disengagement scores for the subjects included in the analysis, a 2×2 repeated measures ANOVA was conducted, where the first 2 in the 2×2 represented the two groups, the intervention group and the control group, and the second 2 in the 2×2 represented the attention bias, which included attention orienting and attention disengagement. Attention orienting was the mean reaction time in the inattentional allocation condition minus the mean reaction time in the congruent condition; attention disengagement was the mean reaction time in the incongruent condition minus the mean reaction time in the inattentional allocation condition. Subsequent Bonferroni tests were used for multiple comparisons. When the spherical shape requirement was not met, Greenhouse–Geisser-corrected p values ​​were reported. A two-sided alpha of 0.5 was set for all analyses. The measured effect sizes were expressed as partial Eta squares. Data exceeding three standard deviations, whether positive or negative, were removed.

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