A traditional Chinese medicine spray for treating qi stagnation and blood stasis syndrome of neck, waist and limbs of dogs and cats

Abstract

The application discloses a traditional Chinese medicine spray for treating qi stagnation and blood stasis syndrome of neck, waist and limbs of dogs and cats, and belongs to the technical field of traditional Chinese veterinary medicine. The traditional Chinese medicine spray mainly comprises the following components: Radix Cyathulae, Angelica sinensis, Radix Paeoniae Rubra, Ligusticum chuanxiong, borneol, safflower, Radix Rehmanniae, Platycodonis, Semen Persicae, Fructus Aurantii, Bupleurum chinense, and Radix Glycyrrhizae. The application extracts effective components from raw medicinal materials by adopting mutual compatibility of various traditional Chinese medicines, and prepares the spray. The spray has a remarkable effect on qi stagnation and blood stasis syndrome of neck, waist and limbs of dogs and cats, has the functions of activating blood and resolving stasis, relieving pain and dredging meridians, is convenient to use, and can directly reach the affected part and has a remarkable medicinal effect compared with oral preparations.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of Chinese veterinary medicine application, and particularly relates to a Chinese medicine spray for treating qi stagnation and blood stasis syndrome of neck, waist and limbs of dogs and cats. BACKGROUND

[0002] With the increase of pet aging degree, the clinical proportion of lumbar disease, cervical disease and limb joint disease is increasing, which brings a lot of troubles to pets and pet parents. Western veterinarians believe that the lumbar and cervical vertebrae, limb joint disease may be caused by low potassium, calcium deficiency, cervical and lumbar vertebrae disease, spinal cord disease, joint sprain and the like. However, Chinese veterinarians believe that the pain of cervical vertebrae, lumbar vertebrae and limb joints is qi stagnation and blood stasis syndrome.

[0003] For the treatment of mild and moderate lumbar, cervical and limb joint diseases, western medicine often adopts conservative treatment, such as using steroids and non-steroidal anti-inflammatory analgesics to inhibit the occurrence of secondary inflammation and reduce edema. Although this method can temporarily relieve pain and swelling, it cannot cure the disease, and may even aggravate the disease over a long period of time. However, Chinese veterinarians believe that blood stasis leads to stasis and cannot generate new blood, and without new blood, nourishment is poor. Therefore, the pain of lumbar vertebrae, cervical vertebrae and limbs is qi stagnation and blood stasis syndrome caused by external invasion. Due to qi stagnation and blood stasis of local tissues and obstruction of meridian circulation, limbs appear weak and painful, so drugs for activating blood and resolving stasis, pain relief and meridian dredging should be used in treatment.

[0004] Therefore, it is necessary to develop a Chinese medicine preparation for treating qi stagnation and blood stasis syndrome of pet lumbar vertebrae, cervical vertebrae and limbs. SUMMARY

[0005] The purpose of the present application is to provide a Chinese medicine spray for conservative treatment of qi stagnation and blood stasis syndrome of neck, waist and limbs of dogs and cats. The Chinese medicine formula is reasonable, the preparation is simple, the effect is obvious, and there is no toxic side effect, so that the qi stagnation and blood stasis syndrome of neck, waist or limbs of dogs and cats can be treated fundamentally.

[0006] To achieve the above-mentioned purpose, the specific technical scheme of the present application is as follows:

[0007] In the first aspect of the present application, a Chinese medicine spray for treating qi stagnation and blood stasis syndrome of neck, waist and limbs of dogs and cats is provided, which comprises the following Chinese medicine components: Radix Achyranthis Bidentatae, Angelica sinensis, Radix Paeoniae Rubra, Rhizoma Chuanxiong, borneol, safflower, Radix Rehmanniae, Platycodonis, Semen Persicae, Fructus Aurantii, Radix Bupleuri and Glycyrrhiza.

[0008] The Chinese medicine components and their mass fractions contained in the Chinese medicine spray are as follows: Radix Achyranthis Bidentatae 2-25 parts, Angelica sinensis 1-25 parts, Radix Paeoniae Rubra 1-25 parts, Rhizoma Chuanxiong 2-25 parts, borneol 2-25 parts, safflower 1-25 parts, Radix Rehmanniae 2-20 parts, Platycodonis 1-10 parts, Semen Persicae 3-25 parts, Fructus Aurantii 1-15 parts, Radix Bupleuri 1-15 parts and Glycyrrhiza 1-15 parts.

[0009] On the basis of the above scheme, the components are preferably in the following proportions: 3-10 parts of Radix Achyranthis Bidentatae, 2-12 parts of Angelica sinensis, 2-10 parts of Radix Paeoniae Rubra, 3-12 parts of Rhizoma Chuanxiong, 3-12 parts of Borneolum Syntheticum, 2-12 parts of Flos Carthami, 3-10 parts of Radix Rehmanniae, 1-5 parts of Platycodonis, 4-12 parts of Semen Persicae, 1-8 parts of Fructus Aurantii, 1-8 parts of Radix Bupleuri, and 1-8 parts of Radix Glycyrrhizae.

[0010] On the basis of the above scheme, the components are more preferably in the following proportions: 4-8 parts of Radix Achyranthis Bidentatae, 3-9 parts of Angelica sinensis, 2-6 parts of Radix Paeoniae Rubra, 4-9 parts of Rhizoma Chuanxiong, 4-9 parts of Borneolum Syntheticum, 3-9 parts of Flos Carthami, 4-8 parts of Radix Rehmanniae, 1-2 parts of Platycodonis, 5-10 parts of Semen Persicae, 2-5 parts of Fructus Aurantii, 1-5 parts of Radix Bupleuri, and 1-5 parts of Radix Glycyrrhizae.

[0011] On the basis of the above scheme, the components are further preferably in the following proportions: 6 parts of Radix Achyranthis Bidentatae, 5 parts of Angelica sinensis, 3 parts of Radix Paeoniae Rubra, 5 parts of Rhizoma Chuanxiong, 5 parts of Borneolum Syntheticum, 5 parts of Flos Carthami, 5 parts of Radix Rehmanniae, 2 parts of Platycodonis, 7 parts of Semen Persicae, 3 parts of Fructus Aurantii, 2 parts of Radix Bupleuri, and 2 parts of Radix Glycyrrhizae.

[0012] In a second aspect of the present application, a preparation method of a traditional Chinese medicine spray for treating Qi stagnation and blood stasis syndrome of the neck, waist and limbs of dogs and cats is provided, which is performed according to the following steps:

[0013] (1) The raw materials are weighed according to the mass fraction. After the Radix Paeoniae Rubra, Rhizoma Chuanxiong and Angelica sinensis are crushed, they are soaked in 1-10 times the amount of ethanol for 3 times, each time for 3 days, and stirred every 12 hours. The soaking liquid is combined and filtered. The ethanol is recovered, and the residue is dried under reduced pressure and crushed to obtain the soaking material, which is reserved.

[0014] (2) The Semen Persicae, Platycodonis, Fructus Aurantii and Radix Bupleuri are distilled with 10-20 times the amount of water to extract the volatile oil and saturated aromatic water, which are reserved.

[0015] (3) The Flos Carthami, Radix Rehmanniae and Radix Glycyrrhizae are decocted with 5-15 times the amount of 50% ethanol for 2 times, each time for 1-2 hours. The filtrate is collected, and the ethanol is recovered until no alcohol smell is left. The filtrate is further concentrated to a relative density of 1.18-1.22 (70±5°C) to obtain the concentrated liquid, which is reserved.

[0016] (4) The Borneolum Syntheticum is dissolved in an appropriate amount of ethanol, and then mixed with the soaking material obtained in step (1) and the concentrated liquid obtained in step (3). The mixture is stirred to dissolve to obtain the medicinal liquid, which is reserved.

[0017] (5) The antioxidant and transdermal agent are added to the medicinal liquid obtained in step (4), and the volatile oil and aromatic water obtained in step (2) are stirred uniformly. The mixture is prepared into a solution with 1 g of crude drug per ml by adding 50% ethanol. The solution is stored in a cold place for 48-72 hours, filtered, and then filled into a spray bottle to obtain the spray.

[0018] The antioxidant in step (5) is used in an amount of 1% to 3% of the total volume of the traditional Chinese medicine spray, and the transdermal agent is used in an amount of 2% to 5% of the total volume of the traditional Chinese medicine spray.

[0019] The antioxidant in step (5) is sodium bisulfite, and the transdermal agent is one of laurazepam or sodium myristyl sulfate.

[0020] In a third aspect of the present application, the application of the traditional Chinese medicine spray for treating qi stagnation and blood stasis syndrome of the neck, waist and limbs to the preparation of a medicine for treating qi stagnation and blood stasis syndrome of the neck, waist and limbs of dogs and cats and pain caused by qi stagnation and blood stasis syndrome is provided.

[0021] In the present application, the red peony root, the Chinese gallnut and the Szechuan lovage root can disperse blood stasis, promote blood circulation and regulate qi, the borneol can clear heat and relieve pain and promote the transdermal absorption of the effective components, and all of them are the monarch drugs; the peach kernel and the safflower can activate blood and resolve stasis, dredge the channels and relieve pain; the raw rehmannia can clear heat, cool blood and stop bleeding, and the angelica can tonify blood and activate blood. The two combined use of the pinellia and the fructus aurantii can regulate qi and expand the chest, and the ascending and descending should be appropriate. At the same time, the pinellia has the effect of carrying drugs upward. The radix bupleuri can soothe the liver and relieve depression, regulate qi and remove stagnation, and the combination with the pinellia and the fructus aurantii can make the circulation of qi and blood smooth. The radix glycyrrhizae can harmonize all the drugs.

[0022] The present application has the following beneficial effects:

[0023] 1. In the present application, the effective components in the raw materials are extracted and prepared into a spray by matching various traditional Chinese medicines, and the spray is convenient to use and can directly reach the affected area, and the effect is remarkable compared with oral preparations.

[0024] 2. The present application is convenient and safe to use, and can avoid the risk that pets escape from eating traditional Chinese medicines due to poor palatability, and is not necessary for surgery for joint pain, acute lumbar sprain and cervical spondylosis of pets.

[0025] 3. The traditional Chinese medicine spray in the present application can activate blood and resolve stasis, dredge the meridians, relieve symptoms such as redness and pain, and reduce the levels of pro-inflammatory factors IL-6, IL-1β and TNF-α in the animal body, and has a good therapeutic effect on qi stagnation and blood stasis syndrome of the neck, waist and limbs, and has a broad market prospect. DETAILED DESCRIPTION

[0026] The above content will be further described in detail through the specific embodiments in the form of examples. However, it should not be understood that the scope of the above subject matter is limited to the following examples. Any technology realized based on the content of the present application belongs to the scope.

[0027] Example 1

[0028] A traditional Chinese medicine spray for treating Qi stagnation and blood stasis syndrome of neck, waist and limbs of dogs and cats, the traditional Chinese medicine spray contains the following components and their amounts: Achyranthes bidentata 111.12g, Angelica sinensis 55.56g, Red peony root 55.56g, Chuanxiong 111.12g, Borneol 111.12g, Safflower 55.56g, Rehmannia 111.12g, Platycodon 55.56g, Peach kernel 166.68g, Aurantii 55.56g, Bupleurum 55.56g, Licorice 55.56g.

[0029] Example 2

[0030] A traditional Chinese medicine spray for treating Qi stagnation and blood stasis syndrome of neck, waist and limbs of dogs and cats, the traditional Chinese medicine spray contains the following components and their amounts: Achyranthes bidentata 115.38g, Angelica sinensis 76.92g, Red peony root 76.92g, Chuanxiong 115.38g, Borneol 115.38g, Safflower 76.92g, Rehmannia 115.38g, Platycodon 38.46g, Peach kernel 153.84g, Aurantii 38.46g, Bupleurum 38.46g, Licorice 38.46g.

[0031] Example 3

[0032] A traditional Chinese medicine spray for treating Qi stagnation and blood stasis syndrome of neck, waist and limbs of dogs and cats, the traditional Chinese medicine spray contains the following components and their amounts: Achyranthes bidentata 117.64g, Angelica sinensis 88.23g, Red peony root 58.82g, Chuanxiong 117.64g, Borneol 117.64g, Safflower 88.23g, Rehmannia 117.64g, Platycodon 29.41g, Peach kernel 147.05g, Aurantii 58.82g, Bupleurum 29.41g, Licorice 29.41g.

[0033] Example 4

[0034] A traditional Chinese medicine spray for treating Qi stagnation and blood stasis syndrome of neck, waist and limbs of dogs and cats, the traditional Chinese medicine spray contains the following components and their amounts: Achyranthes bidentata 120g, Angelica sinensis 100g, Red peony root 60g, Chuanxiong 100g, Borneol 100g, Safflower 100g, Rehmannia 100g, Platycodon 40g, Peach kernel 140g, Aurantii 60g, Bupleurum 40g, Licorice 40g.

[0035] Example 5

[0036] A traditional Chinese medicine spray for treating Qi stagnation and blood stasis syndrome of neck, waist and limbs of dogs and cats, the traditional Chinese medicine spray contains the following components and their amounts: Achyranthes bidentata 94.08g, Angelica sinensis 105.84g, Red peony root 70.56g, Chuanxiong 105.84g, Borneol 105.84g, Safflower 105.84g, Rehmannia 94.08g, Platycodon 23.52g, Peach kernel 117.6g, Aurantii 58.8g, Bupleurum 58.8g, Licorice 58.8g.

[0037] Example 6

[0038] A traditional Chinese medicine spray for treating Qi stagnation and blood stasis syndrome of neck, waist and limbs of dogs and cats, the traditional Chinese medicine components contained in the traditional Chinese medicine spray and the amounts thereof are as follows: Achyranthes bidentata 84g, Angelica sinensis 100.8g, Red peony root 84g, Chuanxiong 100.8g, Borneol 100.8g, Safflower 100.8g, Rehmannia 84g, Platycodon 42g, Peach kernel 100.8g, Bitter orange 67.2g, Bupleurum 67.2g, Licorice 67.2g.

[0039] Example 7

[0040] A traditional Chinese medicine spray for treating Qi stagnation and blood stasis syndrome of neck, waist and limbs of dogs and cats, the traditional Chinese medicine components contained in the traditional Chinese medicine spray and the amounts thereof are as follows: Achyranthes bidentata 100g, Angelica sinensis 100g, Red peony root 100g, Chuanxiong 100g, Borneol 100g, Safflower 100g, Rehmannia 80g, Platycodon 40g, Peach kernel 100g, Bitter orange 60g, Bupleurum 60g, Licorice 60g.

[0041] The preparation method of the traditional Chinese medicine spray in Examples 1-7, which comprises the following steps:

[0042] (1) The raw materials are weighed according to the mass fraction. Red peony root, Chuanxiong and Angelica sinensis are crushed and then soaked in 4 times the amount of ethanol for 3 times, 3 days each time, and stirred every 12 hours. The soaking liquid is combined and filtered. The ethanol is recovered and dried under reduced pressure to obtain the soaking material for use;

[0043] (2) Peach kernel, Platycodon, Bitter orange, Bupleurum are added with 14 times water for distillation extraction of volatile oil and saturated aromatic water for use;

[0044] (3) Safflower, Rehmannia and Licorice are added with 10 times the amount of 50% ethanol for decoction 2 times, 1.5 hours each time. The filtrate is collected and the ethanol is recovered until there is no alcohol smell. The filtrate is continuously concentrated to a relative density of 1.18-1.22 (70±5℃) to obtain the concentrated liquid for use;

[0045] (4) Borneol is dissolved in an appropriate amount of ethanol, and then mixed with the soaking material obtained in step (1) and the concentrated liquid above, and stirred to dissolve;

[0046] (5) 1% antioxidant (sodium bisulfite) and 3% transdermal agent (laurazepam in Examples 1-4, and sodium myristyl sulfate in Examples 5-7) are added to the above liquid, and then the volatile oil and aromatic water obtained in step (2) are added. The mixture is stirred uniformly, 50% ethanol is added to 1000ml, and then stored in a refrigerator for more than 48 hours. The mixture is filtered and filled in a spray bottle to obtain the spray.

[0047] Comparative Example 1

[0048] A kind of traditional Chinese medicine spray for treating neck and waist and limbs of dog and cat Qi stagnation and blood stasis, the same as embodiment 4, the difference is that its preparation method is: the raw material is weighed according to mass fraction, 10 times the amount of water is decocted twice, 1.5 h each time, filter, collect filtrate, 70 DEG C reduced pressure concentration is 1.2, add ethanol to make alcohol amount reach 75%, stand for 48 h, filter, the filtrate is recovered to ethanol to no alcohol taste, add ethanol again, make alcohol amount reach 80%, stand for 48 h, filter, the filtrate is recovered to ethanol to no alcohol taste, 1% antioxidant (sodium bisulfite) is added in the above liquid, 3% transdermal agent (laurazepam) is added, constant volume to 1000ml with purified water, cold storage is placed for more than 48 hours, filter, tank in spray bottle is obtained.

[0049] Example 8 clinical effect

[0050] 1. Test grouping and medication: through investigation and cooperation in local pet hospital, 6 dogs diagnosed as Qi stagnation and blood stasis caused neck and waist or limbs bone and joint pain were selected for test, and divided into test group and control group. Table 1 below is the specific grouping and administration.

[0051] Table 1 clinical test grouping and administration

[0052]

[0053]

[0054] 2. Test method: before medication and after treatment, all animals were taken intravenous blood, and after room temperature for 30 min, centrifuged at 3500 r / min for 20 min, and the serum was collected. ELISA kit (Shanghai Enzyme-linked Biotechnology Co., Ltd.) was used to detect the levels of inflammatory factors IL-6, IL-1β and TNF-α in animal serum. The mental state and activity ability of sick dogs before and after medication were recorded. The test results are shown in Table 2:

[0055] Table 2 detection of inflammatory factor levels in animal serum

[0056]

[0057] 3. Test conclusion: for the dogs with neck and waist and limbs Qi stagnation and blood stasis, the treatment of example 1, 4, 7 and comparative example 1, control 1-2 was carried out, it was found that the application could relieve symptoms such as redness and swelling, pain and could reduce the levels of pro-inflammatory factors IL-6, IL-1β and TNF-α in animals. And the effect of example 4 was better than that of example 1, 7 and comparative example 1, control 1 still had obvious symptoms, control 2 was improved, but still not cured. In addition, compared with the control group, the traditional Chinese medicine spray of the application has better therapeutic effect on neck and waist and limbs Qi stagnation and blood stasis.

[0058] As described above, the present application has significant effects of activating blood, relieving pain and reducing swelling, and has advantages of fast analgesic effect, short treatment cycle and convenient use for treating neck, waist and limbs qi stagnation and blood stasis caused by pet contusion and injury.

Claims

1. A traditional Chinese medicine spray for treating qi stagnation and blood stasis syndrome in the neck, waist, and limbs of dogs and cats, characterized in that... The Chinese herbal components and their mass fractions in the herbal spray are as follows: 6 parts of Achyranthes bidentata, 5 parts of Angelica sinensis, 3 parts of Paeonia lactiflora, 5 parts of Ligusticum chuanxiong, 5 parts of Borneol, 5 parts of Carthamus tinctorius, 5 parts of Rehmannia glutinosa, 2 parts of Platycodon grandiflorus, 7 parts of Prunus persica, 3 parts of Citrus aurantium, 2 parts of Bupleurum chinense, and 2 parts of Glycyrrhiza uralensis.

2. A method for preparing a traditional Chinese medicine spray for treating qi stagnation and blood stasis in the neck, waist, and limbs of dogs and cats as described in claim 1, comprising the following steps: (1) Weigh each raw material according to the mass fraction. After crushing the red peony root, chuanxiong rhizome, and angelica root, add 1-10 times the amount of ethanol to soak three times, each time for 3 days, stirring once every 12 hours. Combine the soaking liquids, filter, recover the ethanol, dry under reduced pressure and crush to obtain the soaked material for later use. (2) Extract the volatile oil and saturated aromatic water from peach kernel, platycodon root, immature bitter orange peel, and bupleurum root by distillation with 10-20 times the amount of water, and set aside; (3) Safflower, Rehmannia glutinosa, and Glycyrrhiza uralensis are decocted twice with 5-15 times the amount of 50% ethanol, each time for 1-2 hours. The decoction is filtered, and the filtrate is collected. The ethanol in the filtrate is recovered until there is no alcohol taste. The filtrate is further concentrated to a concentrate with a relative density of 1.18-1.2 at 70±5℃ for later use. (4) Add an appropriate amount of ethanol to dissolve the borneol, then add the impregnation obtained in step (1) and the concentrated solution in step (3), mix evenly and stir to dissolve, and then prepare the medicine solution for later use. (5) Add antioxidants, transdermal agents, volatile oils and aromatic water obtained in step (2) to the drug solution in step (4), stir evenly, add 50% ethanol to prepare a solution of 1g crude drug / ml, refrigerate and stand for 48-72 hours, filter, and bottle into a spray bottle to obtain the spray.

3. The preparation method according to claim 2, characterized in that, The antioxidant mentioned in step (5) is sodium thionite, and the transdermal agent is either laurocapram or sodium tetradecyl sulfate.

4. The preparation method according to claim 2, characterized in that, The amount of antioxidant used in step (5) is 1% to 3% of the total volume of the Chinese medicine spray, and the amount of transdermal agent used is 2% to 5% of the total volume of the Chinese medicine spray.

5. The application of the traditional Chinese medicine spray for treating qi stagnation and blood stasis syndrome in the neck, waist and limbs of dogs and cats as described in claim 1 in the preparation of a medicine for treating qi stagnation and blood stasis syndrome in the neck, waist and limbs of dogs and cats, as well as pain caused by qi stagnation and blood stasis syndrome.

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