A kind of eye protection liquid formula based on Gongju extract and its process

Abstract

This application relates to an eye protection solution formulation and process based on a Gongju extract. This solution is prepared by extracting Gongju with 80% ethanol, to which a certain amount of schizonepeta alata and other excipients are added to form the eye protection solution. The resulting eye protection solution is safe and non-irritating, and compared to existing eye protection solutions, it has superior efficacy in alleviating eye fatigue, conjunctival inflammation, and conjunctival microcirculation.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicine, and particularly relates to a formula of eye protection liquid based on Gongju extract and a process thereof. Background Art

[0002] Chrysanthemum (Flos Chrysanthemi) is one of the commonly used traditional Chinese medicinal materials in my country. It is the dried head inflorescence of the chrysanthemum (Chrysanthemummorifolium Ramat.) of the Asteraceae family. It was first recorded in the "Classic of Mountains and Seas: Zhongshan Jing": "There are many chrysanthemum trees on the mountain of daughters."

[0003] Chrysanthemums are diverse in variety. The book "Ming Yi Bie Lu" (Famous Doctors' Prescriptions) distinguished between medicinal and wild chrysanthemums, classifying sweet and white chrysanthemums as modern medicinal chrysanthemums, while bitter coix is ​​considered wild chrysanthemum. Li Shizhen's "Compendium of Materia Medica" (1580) from the Ming Dynasty states: "There are hundreds of varieties of chrysanthemum, each distinct; sweet chrysanthemums originally grew in the wilds, but are now cultivated." Ling Huan's "Compendium of Materia Medica" from the Qing Dynasty states: "Chrysanthemums from Chuzhou, with single white petals and a sweet taste, are the best. Yellow and white tea chrysanthemums from Hangzhou, with their slightly bitter taste, are second best." The "Supplement to Compendium of Materia Medica" also notes that the sweet chrysanthemums, also known as tea chrysanthemums, grown in Zhejiang and Jiangxi are small and fragrant, while the chrysanthemums from Bozhou are white and slightly smelly.

[0004] Chrysanthemum is a perennial herb with strong adaptability to temperature and a wide distribution. It generally thrives in areas with an average annual temperature of 15°C-20°C. It prefers moisture and light, tolerates fertilizer, is sensitive to drought and waterlogging, and has a long growing season. It is primarily found in parts of Asia and the subtropics, with suitable growing areas being the mountains, plains, and hills between 29°-38°N and 120°-113°E.

[0005] Based on their environment, origin, and processing methods, eight major varieties of medicinal chrysanthemum are categorized as Gongju, Hangju, Haoju, Qiju, Chuju, Huaiju, Jiju, and Huangju. Due to artificial selection and geographical isolation, hybridization is rare among medicinal chrysanthemums, resulting in significant morphological variation. Gongju has very few tubular flowers and a higher number of ray-shaped flowers compared to other chrysanthemums. Huangshan City has a long history of Gongju cultivation and a large cultivated area. Some plants have exhibited some phenotypic variation, including multiple blooms, early flowering, and altered flower shapes. There are also cases of germplasm degradation.

[0006] The 2020 edition of the "Chinese Pharmacopoeia" records five sources of medicinal chrysanthemum: "Boju, Chuju, Gongju, Hangju and Huaiju. It is sweet, bitter, slightly cold in nature, and enters the lung and liver meridians. It has the effects of dispersing wind and heat, calming the liver and improving eyesight, clearing heat and detoxifying. It is mainly used to treat wind-heat colds, headaches and dizziness, red and swollen eyes, blurred vision, sores, carbuncles and swelling."

[0007] Gongju originated in the Song Dynasty and flourished during the Ming and Qing dynasties. As a tribute medicinal herb, it was known as "gongju." The Deju recorded in the "Deqing New County Chronicles" is one of the tribute chrysanthemum varieties, renowned for its fragrant and sweet aroma. It gradually disappeared after the founding of the People's Republic of China. With the development of cultivation, harvesting, and processing technologies, the resource problem of medicinal chrysanthemum has been resolved. Through natural and artificial selection, relatively stable production areas and cultivated varieties have emerged, such as Huaiju in Henan, Gongju and Chuju in Anhui, and Hangju in Zhejiang. In modern times, varieties used for both medicinal and tea purposes are primarily Hangbai and Gongju.

[0008] Huangshan Gongju is currently mainly distributed in Shexian, Xiuning, Jixi, Yixian and other areas of Huangshan in Anhui Province. The main production area is located in Shexian, Anhui Province (200-600m above sea level, mountainous terrain, 118.4° east longitude, 29.8° north latitude; annual average temperature 16.0℃; annual precipitation 1800mm; frost-free period of about 230 days).

[0009] Gongju is a perennial herbaceous plant of the genus Chrysanthemum in the Asteraceae family. It grows 60-100 cm tall. The plant is covered with white hairs; its stems are light green or brown, erect and branched, with a semi-lignified base. Its leaves are simple, alternate, ovate to oblong, with notched and serrated edges. The leaves are covered with white hairs and have auricles. Its inflorescence heads are terminal or axillary, mostly solitary and arranged in a sparse raceme. Achenes are rudimentary (propagation is by root division or grafting). Flowering occurs from October to November.

[0010] Its head inflorescence is oblate or irregularly spherical and relatively dense; the inflorescence is 1.5-2.5cm in diameter and has no stalk; the disc-shaped involucre is 3-4 layers, light green herbaceous, and covered with soft hairs; the ligulate flowers are 13-16 layers, white or off-white, reflexed at the top, slightly involute and wrinkled at the edges, usually without glandular dots; the tubular flowers are fewer, light yellow, bisexual, located in the center, hidden in the ligulate flowers, and have 5 teeth at the top; the achenes are underdeveloped and have no pappus.

[0011] Ordinary chrysanthemum tea has excellent health benefits. Iced chrysanthemum tea with rock sugar or honey has cooling, heat-reducing, and throat-soothing properties. This is because the chrysanthemum flowers, leaves, and stems contain a rich supply of nutrients, including volatile oils, amino acids, and vitamins A and B. These nutrients dispel wind and heat, inhibit pathogens, and are effective against Escherichia coli, Streptococcus, and Staphylococcus aureus. It also has a relatively stable blood pressure-lowering effect and prevents cardiovascular and cerebrovascular ischemia and thrombosis. Chrysanthemum can be used as an adjunct therapy for those suffering from hypertension, coronary heart disease, migraines, lymphadenitis, and acute conjunctivitis.

[0012] The greatest health benefit of chrysanthemum is its eye protection and vision. People have long known that chrysanthemum has eye-protecting properties. Drinking too much water before bed can cause puffy eyes the next morning. Applying chrysanthemum tea to a cotton ball soaked in cotton can quickly reduce swelling. Regular consumption of chrysanthemum tea can alleviate symptoms of eye fatigue, and drinking three to four cups daily can also help improve vision.

[0013] Chrysanthemum is rich in vitamin A, an important substance for maintaining eye health. It can be used as an adjunct treatment for blurred vision, venous congestion in the retina, optic neuritis, and retinitis. It can also be used in combination with cooling herbs like mint, horsetail, and quinoa for anti-inflammatory effects in keratitis, conjunctivitis, and pharyngitis.

[0014] People who spend all day with computers or electrical appliances experience increased eye fatigue due to exposure to radiation. Regularly drinking chrysanthemum tea and placing a pot of daisies in the teapot can alleviate eye fatigue, stabilize blood pressure, and improve mood. Chrysanthemum is known to dispel wind and heat, clear the liver and purge fire, and nourish yin and improve eyesight. Therefore, it can be used to treat eye swelling and pain caused by wind-heat in the liver meridian or upward movement of liver fire. It is often used with mulberry leaves, cassia seeds, and gentian to dispel wind, clear the liver, and improve eyesight. For those with liver and kidney deficiency and blurred vision, it can be combined with wolfberry, Rehmannia root, and Cornus officinalis, as in Qiju Dihuang Wan, which nourishes the liver and kidneys and improves yin and eyesight.

[0015] Gongju nourishes the liver and improves eyesight, dispels wind and heat, and provides cooling and detoxification. It can treat sores, swelling, colds, arteriosclerosis, and high blood pressure. Regular consumption of chrysanthemum tea or wine can "cleans the five internal organs, detoxify, and improve health," potentially promoting longevity and beauty. Currently, Gongju is primarily used in tea beverages and health supplements, though its use remains relatively simple.

[0016] Huangshan Gongju (Gongju) is rich in a variety of active ingredients, including flavonoids, organic acids, volatile oils, amino acids, sugars, and trace elements. The main active ingredients in the flowers of Gongju are flavonoids and chlorogenic acid compounds. Flavonoids have antioxidant properties and are known to treat cardiovascular disease. The antioxidant activity of Huangshan Gongju water extract is dose-dependent with flavonoids. Therefore, drinking Gongju tea regularly can improve free radical scavenging capacity and thus slow aging. Flavonoids in Gongju can also increase SOD activity in liver tissue and reduce malondialdehyde levels, thereby protecting the liver.

[0017] Alexine was isolated from the pods of Alexia serrata (Leguminosae) in 1988. Chemical name: (1R, 2R, 3R, 7S, 7aS)-3-(hydroxymethyl)hexahydro-1H-pyrrolidine-1,2,7-triol, molecular formula: C8H 15 NO4, relative molecular mass: 189, chemical structure is as follows:

[0018]

[0019] Literature reports that safflower oil has an inhibitory effect on glycosidase, with an IC50 of 5.9×10 -5 mol / L, IC for fungal glucan 1,4-α-glucosidase 50 1.1×10 -5 mol / L.

[0020] However, there is no report on other activities of spartine in the prior art. Summary of the Invention

[0021] The patent applicant of the present invention has conducted in-depth research on Gongju and found that after preparing Gongju extract with 80% ethanol and combining it with sophora flavescens and other auxiliary materials, the resulting eye protection solution is safe and non-irritating. Compared with the existing eye protection solution that only uses chrysanthemum extract, it has better therapeutic effects on improving eye fatigue, conjunctivitis, conjunctival microcirculation, etc.

[0022] This application first discloses a method for preparing a Gongju extract, which comprises the following steps:

[0023] Step 1) taking a sample of Huangshan Gongju into a drying oven, adjusting the drying oven temperature to 50° C. and drying for 48 hours, then taking it out and crushing it with a grinder and sieving it (100 mesh);

[0024] Step 2) taking 100 g of Huangshan Gongju crushed sample, adding 12 times the amount of 80% ethanol, soaking for 3 hours, and filtering to obtain a filtrate;

[0025] Step 3) taking the filter residue from step 2), adding 10 times the amount of 80% ethanol, boiling for 2 hours, and filtering to obtain a filtrate;

[0026] Step 4) taking the filter residue from step 3), adding 6 times the amount of 80% ethanol, boiling for 1.5 hours, and filtering to obtain a filtrate;

[0027] Step 5) Combine the filtrates from step 2), step 3) and step 4), filter with a 0.45um microporous membrane, and add 5% PVP

[0028] K10, slightly boil and concentrate until there is no alcohol taste, add purified water to adjust the relative density to 1.1-1.2g / ml at room temperature to obtain Gongju extract.

[0029] This application also discloses an eye protection solution based on the above-mentioned Gongju extract, and its preparation process is as follows:

[0030] Step 1) Take the Gongju extract obtained in Example 1 and add purified water at a volume ratio of 1:9;

[0031] Step 2) adding scorbutic acid, 2% taurine, 0.2% sodium hyaluronate, and 0.01% benzalkonium chloride according to mass percentage to the solution obtained in step 1), stirring to dissolve;

[0032] Step 3) The solution obtained in step 2) is adjusted to a pH of 6.5-7.3 with 0.1M hydrochloric acid or sodium hydroxide aqueous solution, and the solution is divided into portions to obtain a finished product.

[0033] The invention is characterized in that the sophorine is added in an amount of 0.1-0.5% by mass.

[0034] Finally, the present application discloses the use of the above-mentioned eye protection liquid for preparing eye protection drugs, and the said use refers to the efficacy of improving eye fatigue, improving conjunctivitis, improving conjunctival microcirculation, etc.

[0035] The present invention provides an eye protection solution by extracting chrysanthemum with 80% ethanol to prepare a chrysanthemum extract, and then adding a certain amount of schizonepeta and other auxiliary materials to the extract. The resulting eye protection solution is safe and non-irritating, and compared with existing eye protection solutions, has better efficacy in improving eye fatigue, conjunctivitis, and conjunctival microcirculation. DETAILED DESCRIPTION

[0036] The present invention is further described below with reference to specific examples, which, however, are not intended to limit the present invention in any way. Unless otherwise specified, the reagents, methods, and equipment used in the present invention are conventional reagents, methods, and equipment in the art.

[0037] Unless otherwise specified, the reagents and materials used in the following examples were commercially available.

[0038] Example 1 Preparation of Gongju Extract

[0039] Step 1) taking a sample of Huangshan Gongju into a drying oven, adjusting the drying oven temperature to 50° C. and drying for 48 hours, then taking it out and crushing it with a grinder and sieving it (100 mesh);

[0040] Step 2) taking 100 g of Huangshan Gongju crushed sample, adding 12 times the amount of 80% ethanol, soaking for 3 hours, and filtering to obtain a filtrate;

[0041] Step 3) taking the filter residue from step 2), adding 10 times the amount of 80% ethanol, boiling for 2 hours, and filtering to obtain a filtrate;

[0042] Step 4) taking the filter residue from step 3), adding 6 times the amount of 80% ethanol, boiling for 1.5 hours, and filtering to obtain a filtrate;

[0043] Step 5) Combine the filtrates from step 2), step 3) and step 4), filter with a 0.45um microporous membrane, and add 5% PVP

[0044] K10, slightly boil and concentrate until there is no alcohol taste, add purified water to adjust the relative density to 1.1-1.2g / ml at room temperature to obtain Gongju extract.

[0045] Example 2 Preparation of Compound Gongju Eye Protection Liquid

[0046] Step 1) Take the Gongju extract obtained in Example 1 and add purified water at a volume ratio of 1:9;

[0047] Step 2) adding 0.1% scorbutic acid, 2% taurine, and 0.2% sodium hyaluronate to the solution obtained in step 1) by mass percentage;

[0048] 0.01% benzalkonium chloride, stir to dissolve;

[0049] Step 3) The solution obtained in step 2) is adjusted to a pH of 6.5-7.3 with 0.1M hydrochloric acid or sodium hydroxide aqueous solution, and the solution is divided into portions to obtain a finished product.

[0050] Comparative Example 1: Preparation of Gongju Extract Using Purified Water as Solvent and Then Preparing Eye Protection Solution

[0051] Step 1) taking a sample of Huangshan Gongju into a drying oven, adjusting the drying oven temperature to 50° C. and drying for 48 hours, then taking it out and crushing it with a grinder and sieving it (100 mesh);

[0052] Step 2) taking 100 g of Huangshan Gongju crushed sample, adding 12 times the amount of purified water, soaking for 3 hours, and filtering to obtain a filtrate;

[0053] Step 3) taking the filter residue from step 2), adding 10 times the amount of purified water, boiling for 2 hours, and filtering to obtain a filtrate;

[0054] Step 4) taking the filter residue from step 3), adding 6 times the amount of purified water, boiling for 1.5 hours, and filtering to obtain a filtrate;

[0055] Step 5) Combine the filtrates from step 2), step 3) and step 4), filter with a 0.45um microporous membrane, and add 5% PVP

[0056] K10, slightly boil and concentrate until there is no alcohol taste, add purified water to adjust the relative density at room temperature to 1.1-1.2g / ml to obtain Gongju extract.

[0057] Step 6) Take the Gongju extract obtained in step 5) and add purified water at a volume ratio of 1:9;

[0058] Step 7) adding 0.1% scorbutic acid, 2% taurine, 0.2% sodium hyaluronate, and 0.01% benzalkonium chloride according to mass percentage to the solution obtained in step 6) and stirring to dissolve;

[0059] Step 8) The solution obtained in step 7) is adjusted to a pH of 6.5-7.3 with 0.1 M hydrochloric acid or sodium hydroxide aqueous solution, and the solution is divided into portions to obtain a finished product.

[0060] Comparative Example 2 Eye protection solution with different amounts of sophorine added

[0061] Step 1) Take the Gongju extract obtained in Example 1 and add purified water at a volume ratio of 1:9;

[0062] Step 2) adding 2% taurine, 0.2% sodium hyaluronate, and 0.01% benzalkonium chloride according to mass percentage to the solution obtained in step 1), stirring to dissolve;

[0063] Step 3) Take 3 portions of the medicinal solution obtained in step 2) and add 0, 0.2%, and 0.5% of sophorine respectively according to the mass fraction.

[0064] Step 4) The pH of the solution obtained in step 3) was adjusted to 6.5-7.3 with 0.1M hydrochloric acid or sodium hydroxide aqueous solution, and the solution was divided into several portions to obtain Comparative Example 2 Sample 1, Sample 2, and Sample 3.

[0065] Example 3 Irritation test of eye protection solution on rabbit conjunctiva

[0066] Ten rabbits were stained with 1% sodium fluorescein (approximately 0.02 mL) in both eyes before grouping on the first day of administration. Slit lamp microscopy was performed within 10 minutes after dyeing to exclude animals with eye irritation, corneal defects, dyeing, and conjunctival damage. 0.1 ml of the compound Gongju eye protection solution (Sample 3, Comparative Example 2) was then instilled into the conjunctival sac of the left eye, and 0.1 ml of normal saline was instilled into the right eye. Both eyes were passively closed for 10 seconds, three times a day, for 7 consecutive days. Mucosal reactions in the eyes were observed for 7 days after the final dose.

[0067] After the first dose and during the recovery period each day, the rabbits were immobilized, their lower eyelids pulled open, and one drop (approximately 0.02 mL) of 1% sodium fluorescein solution was instilled into each eye for staining. Within 10 minutes after staining, the cornea, iris, and conjunctiva of the rabbits were observed under a slit lamp microscope for ocular irritation. Scores were calculated according to the ocular irritation scoring criteria in Table 1. The average score was used to determine the irritation level of the left and right eyes of each group: 0-3 points indicated no irritation; 4-8 points indicated mild irritation; 9-12 points indicated moderate irritation; and 13-16 points indicated severe irritation. The results are shown in Table 2.

[0068] Table 1 Free eye irritation reaction score standard

[0069]

[0070] Table 2 Comparative Example 2 Sample 3 Compound Gongju Eye Protection Liquid Irritation to Rabbit Eyes

[0071] Group n Eye contact cornea conjunctiva iris Eye irritation conclusion Comparative Example 2 Sample 3 Compound Gongju Eye Protection Liquid 10 Left 0 0 0 Non-irritating Normal saline 10 right eye 0 0 0 Non-irritating .

[0072] Example 4 Effects of Example 2 Eye Protection Solution and Comparative Example Samples on Capsaicin-Induced Conjunctival Inflammation in Rabbits

[0073] Forty-eight Japanese large-eared rabbits were randomly divided into eight groups: a normal group; a model group; a normal saline control group; the group treated with Example 2, the group treated with Comparative Example 1, and the groups treated with Samples 1, 2, and 3 of Comparative Example 2. Except for the normal group, 0.1 ml of 2% capsaicin was instilled into the left eye of the animals in all other groups to establish a conjunctivitis model.

[0074] The control group, the group treated with saline solution, the group treated with Example 2, the group treated with Comparative Example 1, and the groups treated with Samples 1, 2, and 3 of Comparative Example 2 were each instilled with 0.1 ml of the drug solution at 0.5, 2, 8, 24, 48, and 72 hours after inflammation was induced. The degree of conjunctival inflammation in each of the above time periods was scored according to the eye irritation response scoring criteria in Table 1. Scoring results as follows:

[0075] Group 0h 0.5h 2h 8h 24h 48h Normal group 0 0 0 0 0 0 Model Group 0 7.5±0.8 6.4±0.7 2.3±0.5 1.2±0.3 0.6±0.2 Normal saline control group 0 7.7±1.0 6.3±0.6 2.5±0.4 1.5±0.7 0.5±0.4 Example 2 Dosage Group 0 7.8±1.2 3.6±1.8 1.7±0.5 0.7±0.5 0.3±0.2 Comparative Example 1: Dosage Group 0 7.8±1.7 5.4±1.2 2.3±0.9 0.9±0.6 0.4±0.4 Comparative Example 2 Sample 1 group 0 7.4±1.5 4.8±0.6 2.1±0.6 0.8±0.4 0.4±0.3 Comparative Example 2 Sample 2 groups 0 7.5±1.1 3.7±0.5 1.5±0.4 0.6±0.5 0.2±0.2 Comparative Example 2 Sample 3 groups 0 7.4±1.6 3.7±0.6 1.6±0.3 0.6±0.3 0.2±0.1 .

[0076] The results showed that 0.5 hours after inflammation, except for the normal group, the conjunctival scores of all groups of animals were 7.3 to 7.8, which was a strong inflammatory response; 24 hours after inflammation, due to the natural recovery of the eye inflammation response in the model group, the difference in inflammatory response between the groups was no longer significant.

[0077] Compared with the normal saline group, after the administration of the other groups, the inflammation of the eye conjunctiva all alleviated to varying degrees at different times. Wherein embodiment 2, comparative example 2 sample 2 and sample 3 groups, compared with comparative example 2 sample 1 (without adding sophorine), inflammation improvement was more significant. Specifically manifested in 2-48h after administration, the conjunctiva score of this group of test animals was all lower than the other groups. Proof is based on the addition of sophorine to the Gongju extract described in the application can significantly improve the inflammation of the eye conjunctiva, and the inflammation improvement situation is more significant as the increase of sophorine mass fraction.

[0078] It is worth noting that the comparative example 1 group is an eye protection solution prepared by adding safflower oil and preparing the chrysanthemum extract with purified water as the solvent. Its conjunctivitis improvement effect is inferior to that of Example 2 and each sample of comparative example 2, which proves that the chrysanthemum extract prepared by 80% ethanol extraction and safflower oil have a better conjunctivitis improvement effect and can better improve conjunctivitis.

[0079] Example 5 Effects of the eye protection solution of Example 2 and the comparative example sample on the microcirculation of the conjunctiva of rabbits.

[0080] Forty-eight rabbits were divided into a normal group, a model group, a normal saline control group, an eye protection solution of Example 2 and a sample of Comparative Example 1, and samples 1-3 of Comparative Example 2, for a total of eight groups, with six rabbits in each group. The rabbits were anesthetized with urethane and fixed on a small operating table. The head was positioned in the lateral position with the eyes upward and the eyelids parted, and the eyes were illuminated with a cold light source. Changes in the conjunctival microcirculation were observed using a projection microscope and a television display screen.

[0081] Measure the microvascular blood flow velocity (number of micrometers of red blood cells moving per second) under normal conditions and the blood flow velocity within a certain area (1.67mm 2 ) Capillary network number. Except for the normal group, all other experimental animals were injected with 10% high-molecular-weight dextran (15 ml / kg) into the pretibial vein to induce microcirculatory impairment. The improvement of the above indicators in each group of experimental animals after administration (twice daily for one week) was observed. The differences before and after administration were compared. The normal saline control group was treated in the same manner as above. as follows:

[0082]

[0083] The experimental results show that under normal conditions, the capillary caliber is about 2 to 3 times the diameter of red blood cells, the red blood cells flow in a granular manner, there is no white blood cell aggregation, and the flow rate per second is about 150.3±15.4μm / s (n=6, x±s). 2 The number of capillary sites was approximately 7.18 ± 1.1 (n = 6, x ± s);

[0084] After intravenous injection of high-molecular-weight dextran, the blood flow rate slowed down significantly, the red blood cell flow became granular and flocculent with stasis, white blood cell aggregation occurred, and the capillary network was significantly reduced.

[0085] Compared with the normal saline group, after the administration of the remaining groups, the conjunctival microcirculation of the eye is alleviated to varying degrees. Wherein embodiment 2, comparative example 2 sample 2 and sample 3 groups, compared with comparative example 2 sample 1 (without adding sophorine), the improvement of conjunctival microcirculation is more significant. Specifically manifested as after administration, the microvascular blood flow velocity (μm / s) and microvascular intersection points (individual / 1.67mm2) of the eye of the group of test animals, compared with the remaining groups, have a significant increase. Prove that adding sophorine based on the Gongju extract described in the application can significantly improve eye conjunctival inflammation, and the improvement of conjunctival microcirculation is more significant with the increase of sophorine mass fraction.

[0086] It is worth noting that the comparative example 1 group is an eye protection solution prepared by adding chrysanthemum extract prepared with purified water as solvent and then adding safflower oil. Its conjunctival microcirculation improvement effect is inferior to that of Example 2 and each sample of comparative example 2, which proves that the chrysanthemum extract prepared with 80% ethanol extraction and safflower oil have a better effect on improving conjunctivitis, can better improve conjunctival microcirculation, and thus improve the symptoms of eye fatigue.

[0087] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the spirit and principles of the present invention should be considered as equivalent replacement methods and are included in the scope of protection of the present invention.

Claims

1. An eye protection solution based on Gongju extract, characterized in that: It is prepared with Gongju extract, scutellaria baicalensis and pharmaceutically acceptable excipients. The chemical structure of scutellaria baicalensis is as follows: ； The sophorine is added in an amount of 0.1-0.5% by mass; The preparation method of the Gongju extract is as follows: Step 1) Place a sample of Huangshan Gongju in a drying oven, adjust the oven temperature to 50°C, dry for 48 hours, remove from the oven, and grind it through a 100-mesh sieve using a grinder; Step 2) Take 100g of crushed Huangshan Gongju sample, add 12 times the amount of 80% ethanol, soak for 3 hours, and filter to obtain a filtrate; Step 3) Take the filter residue from step 2), add 10 times the amount of 80% ethanol, boil for 2 hours, and filter to obtain a filtrate; Step 4) Take the filter residue from step 3), add 6 times the amount of 80% ethanol, boil for 1.5 hours, and filter to obtain a filtrate; Step 5) Combine the filtrates obtained in step 2), step 3) and step 4), filter through a 0.45 μm microporous filter membrane, add 5% PVPK10, concentrate with slight boiling until there is no alcohol taste, add purified water until the relative density at room temperature is 1.1-1.2 g / ml, and obtain the Gongju extract.

2. The eye protection solution according to claim 1, wherein The sophorine is added in an amount of 0.1% by mass.

3. The eye protection solution according to any one of claims 1 to 2, characterized in that The preparation steps of the eye protection solution are as follows: Step 1) Take the Gongju extract obtained in claim 1 and add purified water at a volume ratio of 1:9; Step 2) adding scorbutic acid, 2% taurine, 0.2% sodium hyaluronate, and 0.01% benzalkonium chloride according to mass percentage to the solution obtained in step 1), stirring to dissolve; Step 3) The solution obtained in step 2) is adjusted to a pH of 6.5-7.3 with 0.1M hydrochloric acid or sodium hydroxide aqueous solution, and the solution is divided into portions to obtain the finished product.

4. The eye protection solution according to any one of claims 1 to 2, for use in preparing eye protection drugs.

5. The eye protection solution according to claim 4, wherein The eye protection medicine is used for improving eye fatigue, improving conjunctival inflammation, and improving conjunctival microcirculation.

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