A multi-enzyme cascade catalytic reaction system for synthesizing trehalose-6-phosphate and its application

CN119082068BActive Publication Date: 2025-09-02DALIAN UNIV OF TECH
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Patent Information

Application Number
CN202411325552.X
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-09-23
Publication Date
2025-09-02
Estimated Expiration
2044-09-23

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Abstract

The present invention discloses a multi-enzyme cascade catalytic reaction system for synthesizing trehalose-6-phosphate and its application, belonging to the field of biocatalysis technology. The present invention constructs a multi-enzyme cascade catalytic reaction system containing maltose phosphorylase, polyphosphate glucokinase, trehalose-6-phosphate phosphorylase, and polyphosphate kinase through in vitro recombinant expression of the enzymes. The multi-enzyme cascade catalytic reaction system catalyzes the synthesis of trehalose-6-phosphate from the raw material maltose. The method of the present invention has a high raw material utilization rate, and the conversion efficiency of maltose to the target product trehalose-6-phosphate reaches 90%, which is the highest level currently reported domestically and internationally, laying the foundation for the industrial synthesis of trehalose-6-phosphate.
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Citation Information

Patent Citations

  • Method for producing trehalose hexaphosphate

    CN114854807A

  • Strain for catalytic synthesis of trehalose-6-phosphoric acid

    CN117187150A