Microorganism detection device

Abstract

According to the microbiological detection device provided by the invention, the detachable wire accommodating groove is formed in the bottom of the sensor groove in the working base, the outer diameter of the wire accommodating groove is smaller than that of the sensor groove, a power line placed in the wire accommodating groove can be taken out only by detaching the wire accommodating groove, and the wire accommodating groove can be conveniently taken out. A sensor groove is formed in the working base, a power socket of the sensor is exposed out of the working base, a power line can be directly inserted into the power socket, the sensor located on the sensor groove does not need to be taken out, and the stability and accuracy of the sensor during microorganism inspection are improved. The dropper can suck the correction liquid in the correction liquid container to the sensor for detection.

Description

Technical Field

[0001] The invention relates to a microorganism detection device, in particular to a microorganism detection device with a power line arranged at the bottom of a sensor. Background Art

[0002] Infectious diseases have always been one of the threats to human survival. The casualties they cause are often more tragic than those caused by natural disasters. Effective screening can not only confirm the infection status of the screened person and allow the infected person to be treated quickly, but also confirm the infection status of the social group as a whole. Therefore, screening helps to block the spread of infectious diseases. As infectious diseases change with each passing day, mutate and spread in an endless stream, the demand for screening capacity, popularity and screening speed are also increasing.

[0003] In order to provide rapid screening, a conventional nucleic acid detection device uses a nucleic acid signal amplification method to detect the nucleic acid to be detected (eg, RNA fragments of viruses) in a biological sample (eg, saliva sample).

[0004] The commonly used screening devices mentioned above require the sensor to be connected to a power source in order for the sensor to operate normally. Therefore, a power cord is usually placed at the bottom of the sensor. When testing, the sensor must be taken out first to get the power cord, which may cause damage to the electronic parts of the sensor during the removal process. The sensor must be connected to the power cord during operation, and the sensor connected to the power cord cannot be replaced in the slot of the working base for operation due to the increased volume of the power cord, resulting in the sensor having no specific working platform or environment. In particular, the general nucleic acid screening device must be constantly picked up and connected to the power supply during the screening process, which is very likely to cause shaking or collision. This may lead to inaccurate test results or even wrong test results. Summary of the invention

[0005] In order to provide a detection device that is popular, convenient, easy to use and quick, and solve the problem of inconvenience in use and measurement inaccuracy caused by the above-mentioned wiring, the present invention provides a microorganism detection device that can be connected to the power cord without removing the sensor located on the working base, and can be firmly connected to the power supply in the working base for use. Therefore, it is not easy to shake or be hit during detection, and the detection result can be avoided from being affected by shaking or collision.

[0006] The present invention is a microorganism detection device, comprising a working base, wherein the working base comprises a sampling component slot, a sensor slot, a dropper slot and a correction liquid container slot, wherein the sensor slot has a wire operating slot on one side adjacent to the edge of the working base, wherein the wire operating slot connects the sensor slot with the outside of the working base, and the bottom of the sensor slot has a detachable wire accommodating slot, wherein the outer diameter of the wire accommodating slot is smaller than the outer diameter of the sensor slot, and the wire accommodating slot has a tearing line on the slot wall close to the sensor slot, wherein the tearing line is used to facilitate tearing the wire accommodating slot from the sensor slot, wherein the sampling component slot, the sensor slot, The dropper groove and the correction fluid container groove are arranged on the same plane of the working base; the microorganism detection device also includes a sensor, one side of the sensor has a power socket, the power socket is for inserting a power cord, and the other end of the power cord is connected to a power plug, the sensor is placed flat in the sensor groove, and the power socket corresponds to the wire operation groove, and the power cord is rolled up in the wire accommodating groove; the microorganism detection device also includes a dropper, which is placed in the dropper groove, and the dropper is used to absorb the correction fluid; the microorganism detection device also includes a correction fluid container, which is placed in the correction fluid container groove, and the correction fluid container is filled with the correction fluid.

[0007] In a preferred embodiment, the sampling assembly includes a sampling rod, a lysis container, two cleaning containers and an adsorber.

[0008] In a preferred embodiment, the sampling component slot includes a sampling rod slot, a lysis operation slot, two cleaning operation slots, an adsorber slot, a static slot and a vertical slot. The sampling rod is placed in the sampling rod slot, the lysis container is placed in the lysis operation slot, the cleaning containers are placed in the cleaning operation slots respectively, and the cleaning containers are all filled with cleaning liquid. The adsorber is placed in the adsorber slot or uprightly inserted in the vertical slot, and the adsorber has a magnetic suction component and a shell mounted on the outside of the magnetic suction component.

[0009] In a preferred embodiment, the lysis container is filled with a lysis solution and a plurality of magnetic beads, and the surface of the magnetic beads has an adsorption layer for adsorbing nucleic acids contained in the biological sample.

[0010] In a preferred embodiment, a cracking container fixing groove is concavely provided on the bottom surface of the cracking operation groove, and the outer diameter of the cracking container matches the outer diameter of the cracking container fixing groove, so that the cracking container can be uprightly inserted into the cracking container fixing groove.

[0011] In a preferred embodiment, the bottom surface of the cleaning operation tank is respectively recessed with a cleaning container fixing groove, the static groove has a magnetic attraction portion, the bottom end of the cleaning container has an outer rim and an inner recess, the outer diameter of the cleaning container is matched with the outer diameter of the cleaning container fixing groove or the static groove, so that the cleaning container can be uprightly inserted into the cleaning container fixing groove or the static groove, and the rim is snapped onto the bottom surface of the cleaning container fixing groove or the static groove, and when the cleaning container is inserted into the static groove, the recess can correspond to and adhere to the magnetic attraction portion.

[0012] In a preferred embodiment, the bottom end of the calibration fluid container has an outer rim, and the bottom surface of the calibration operation groove is concavely provided with a calibration fluid container fixing groove, and the outer diameter of the calibration fluid container fixing groove matches the outer diameter of the calibration fluid container, so that the calibration fluid container can be inserted into the calibration fluid container fixing groove, and the rim is snapped onto the bottom surface of the calibration fluid container fixing groove.

[0013] During the operation test, the wire accommodating slot is detached from the sensor slot, and the power cord located inside the wire accommodating slot is taken out. One end of the power cord can be directly inserted into the power socket through the wire operating slot, and the other end is connected to the power plug. In this way, the test can be completed without taking out the sensor. In this way, during the test process, the slot containing the power cord can be directly disassembled to obtain the power cord, so that the sensor can be stably placed flat in the sensor slot, and the power socket of the sensor is exposed, which is convenient for the power cord to be directly plugged in and used, so that the sensor is not easy to shake or collide during the test, which can avoid affecting the test results due to shaking or collision, and can also avoid the power cord from shaking or collision. Poor circuit contact caused by poor circuit contact, thereby achieving the effect of improving the stability of the test results. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required to be used in the description of the embodiments will be briefly introduced below. Obviously, the drawings in the following description are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0015] Figure 1 It is a three-dimensional combination schematic diagram of the microorganism detection device of the present invention.

[0016] Figure 2 It is a three-dimensional exploded schematic diagram of the microorganism detection device of the present invention.

[0017] Figure 3 It is a partial cross-sectional schematic diagram of the sensor of the microorganism detection device of the present invention being placed in the sensor groove.

[0018] Figure 4 It is a three-dimensional schematic diagram of the microorganism detection device of the present invention when in use.

[0019] Figure 5 It is a partial cross-sectional schematic diagram of the lysis container of the microorganism detection device of the present invention.

[0020] Figure 6 It is a partial cross-sectional schematic diagram of a cleaning container of the microorganism detection device of the present invention.

[0021] Figure 7 It is a schematic diagram of a three-dimensional decomposition of the adsorber of the microorganism detection device of the present invention.

[0022] Figure 8 It is a partial cross-sectional schematic diagram of the adsorber of the microorganism detection device of the present invention when in use.

[0023] Fig. 9 It is a partial cross-sectional schematic diagram of the adsorber of the microorganism detection device of the present invention when in use.

[0024] Fig.10 It is a partial cross-sectional schematic diagram of the cleaning container of the microorganism detection device of the present invention placed in a static tank.

[0025] Fig.11 It is a partial cross-sectional schematic diagram of the correction liquid container of the microorganism detection device of the present invention.

[0026] Fig.12 It is a schematic cross-sectional view of the operation of the detection step J of the microorganism detection device of the present invention.

[0027] Fig.13 It is a three-dimensional schematic diagram of the operation of the detection step J of the microorganism detection device of the present invention.

[0028] Fig.14 It is a three-dimensional schematic diagram of the operation of the detection step K of the microorganism detection device of the present invention.

[0029] Reference numerals

[0030] 1 Working base

[0031] 11 Sampling rod slot

[0032] 12 Cracking operation tank

[0033] 121 Cracking vessel fixing slot

[0034] 13. Cleaning tank

[0035] 131 Cleaning container fixing slot

[0036] 14 adsorber tank

[0037] 15. Static Tank

[0038] 151 Magnetic attraction

[0039] 16 Vertical Slot

[0040] 17 Sensor slot

[0041] 171 Wire Operation Slot

[0042] 172 Wire Receiving Slot

[0043] 173 Tearing off the Line

[0044] 18 Dropper slot

[0045] 19 Calibration fluid container

[0046] 191 Calibration fluid container fixing slot

[0047] 2 sampling sticks

[0048] 3. Cracking vessel

[0049] 31 Ring Edge

[0050] 32 recess

[0051] 33 Lysate

[0052] 4 Magnetic beads

[0053] 41 Adsorption layer

[0054] 5. Clean the container

[0055] 51 Ring Edge

[0056] 52 recess

[0057] 53 Cleaning fluid

[0058] 6 Adsorber

[0059] 61 Magnetic parts

[0060] 611 Magnetic Head

[0061] 62 Shell

[0062] 7 Sensors

[0063] 71 Power socket

[0064] 72 Power cord

[0065] 8. Dropper

[0066] 81 Large suction trough

[0067] 82 small suction groove

[0068] 9 Calibration fluid container

[0069] 91 Ring Edge

[0070] 92 Calibration Fluid DETAILED DESCRIPTION

[0071] Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.

[0072] As used herein, the articles "a", "an" and "any" refer to one or more than one (ie, at least one) item without specifying a specific number. For example, "an element" means one element or more than one element.

[0073] As used herein, the term "above" used to describe the location of a structure refers to any surface location of the structure, and is not commonly known as "above" or "above" which has a directionality. The terms "above" and "below" used to describe the location of a structure refer to the directionality of the location of the structure in conventional usage.

[0074] As used herein, the term "inside" describing a structural position refers to a position close to the center of the structural body, or a position that is not exposed during use; the term "outside" refers to a position far from the center of the structural body, or a position that is exposed during use.

[0075] As used herein, the terms "convexly disposed", "concavely disposed", "formed", "extended" or "integrally formed" used to describe a structural combination generally refer to one or more structures being combined into the same body during manufacturing, or corresponding structures generated on the same body due to different positions, shapes and functions.

[0076] The present invention is a microorganism detection device. The complete technical content, characteristics and functions will be clearly presented in the detailed description of the preferred embodiment with reference to the drawings below. In this article, the microorganisms described can be bacteria or viruses, but are not limited to this. In addition, the sampling and testing technology used is to use magnetic beads to adsorb nucleic acids, but is not limited to this. Through the advantage of magnetic beads adsorbing nucleic acids to facilitate transfer, the magnetic beads are transferred in different tubes by specific methods and tools for nucleic acid lysis and purification, or even detection, to avoid the specimen being affected by residual liquid, which will be explained one by one below.

[0077] See also Figure 1 , Figure 1It is a three-dimensional assembly schematic diagram of the microorganism detection device of the present invention, which includes a working base 1, the working base 1 includes a sampling component slot (such as elements 11 to 16), a sensor slot 17, a dropper slot 18 and a calibration liquid container slot 19, and the microorganism detection device also includes a sensor 7, a dropper 8 and a calibration liquid container 9. The sampling component slot, the sensor slot 17, the dropper slot 18 and the calibration liquid container slot 19 are arranged on the same plane of the working base 1, and can accommodate and store the sensor 7, the dropper 8 and the calibration liquid container 9.

[0078] See also Figure 2 , Figure 2 1 is a three-dimensional exploded schematic diagram of the microorganism detection device of the present invention, wherein the microorganism detection device further comprises a sampling component and a sensing component which can be placed on the working base 1. The sampling component comprises a sampling rod 2, a lysis container 3, two cleaning containers 5, and an adsorbent 6. The sampling rod 2 can be a cotton swab. The sensing component comprises a sensor 7, a dropper 8, and a calibration liquid container 9.

[0079] See also Figure 2 , Figure 3 , the sensor slot 17 is close to the edge of the working base 1, and the sensor slot 17 has a wire operation slot 171 on one side adjacent to the edge of the working base 1, and the wire operation slot 171 connects the sensor slot 17 with the outside of the working base 1. The bottom of the sensor slot 17 has a wire accommodating slot 172, and the outer diameter of the wire accommodating slot 172 is smaller than the outer diameter of the sensor slot 17. In a specific embodiment, the wire accommodating slot 172 is recessed downward from the bottom center of the sensor slot 17, so that the sensor slot 17 and the wire accommodating slot 172 form a stepped slot. A tearing line 173 is provided on the slot wall of the wire accommodating slot 172 close to the sensor slot 17, and the tearing line 173 is used to facilitate tearing the wire accommodating slot 172 off the sensor slot 17. In one embodiment, the tear line 173 is an incomplete cutting line formed on the wall of the wire receiving groove 172 when the working base 1 is injection molded, so that the wire receiving groove 172 and the sensor groove 17 are connected when the working base 1 is molded, but when in use, the wire receiving groove 172 can be easily torn off. The sensor 7 is electronic, which is used to detect whether the nucleic acid contained in the biological sample contains the RNA to be tested. The sensor 7 can be a capacitive sensor. One side of the sensor 7 has a power socket 71, and the power socket 71 is for inserting a power cord 72, and the other end of the power cord 72 is connected to a power plug (not shown). The power plug is, for example but not limited to, a computer or a socket. In the packaging state, such as Figure 1As shown, the sensor 7 is placed flat on the sensor slot 17 , and the power socket 71 corresponds to the wire operating slot 171 , and the power cord 72 is rolled up and placed flat on the wire receiving slot 172 .

[0080] In addition, in addition to the design of the tear line 173, in other embodiments, the side of the wire receiving slot 172 can also be combined with the sensor slot 17 through a buckle structure. When the wire receiving slot 172 needs to be disassembled, the wire receiving slot 172 can be disassembled from the sensor slot 17 by simply releasing the buckle structure.

[0081] See also Figure 1 , Figure 2 , Figure 4 The working base 1 can be a thin shell frame made by integral molding, and one or more sampling component grooves are respectively formed on the same surface of the working base 1, which are configured as a sampling rod groove 11 for placing the sampling rod 2, a lysis operation groove 12 for placing the lysis container 3, two cleaning operation grooves 13 for placing the cleaning container 5 respectively, an adsorber groove 14 for placing the adsorber 6, a static groove 15 for uprightly inserting the cleaning container 5, a vertical groove 16 for uprightly inserting the adsorber 6, a sensor groove 17 for placing the sensor 7, a dropper groove 18 for placing the dropper 8 and a correction liquid container groove 19 for placing the correction liquid container 9. The grooves on the working base 1 can be used for horizontally or uprightly placing the sampling component and the sensing component as a supporting and fixing packaging material, which can facilitate the screening operation and is also conducive to the sale of the whole set. In one embodiment, the one-piece formed working base 1 is made of plastic material, and the plastic material is formed by an injection molding machine, so that the grooves on the working base 1 can be quickly formed, and this embodiment is not limited here.

[0082] See also Figure 2 , Figure 4 , Figure 5 The lysis container 3 is a tubular container with a screw cap, and has a rim 31 on the outside of the bottom and a recess 32 on the inside. The lysis container 3 is filled with a lysis solution 33 and a plurality of magnetic beads 4. When left at rest, the magnetic beads 4 can settle and gather at the tip of the recess 32 to facilitate subsequent complete absorption using magnetic force.

[0083] In the packaging state, the cracking container 3 is placed flat in the cracking operation groove 12, and the bottom surface of the cracking operation groove 12 is concavely provided with a cracking container fixing groove 121, and the outer diameter of the cracking container fixing groove 121 is matched with the outer diameter of the cracking container 3. Therefore, in the cracking operation state, the cracking container 3 can be uprightly inserted into the cracking container fixing groove 121, and the rim 31 is snapped on the bottom surface of the cracking container fixing groove 121.

[0084] See also Figure 2 , Figure 5 The lysis solution 33 can lyse the cells of the biological sample and release nucleic acids, such as ribonucleic acid (RNA). The magnetic beads 4 have an adsorption layer 41 on their surface. The adsorption layer 41 includes a silicon material and a primer. The silicon material is, for example, silicon dioxide. The silicon material can be coated on the outer layer of the magnetic beads 4, and the silicon material can be combined with the primer. The primer is a nucleic acid fragment that can specifically bind to the nucleic acid to be detected to carry the nucleic acid to be detected. Therefore, the adsorption layer 41 can effectively adsorb the nucleic acid to be detected that may be contained in the biological sample (such as but not limited to the ribonucleic acid fragment of the Covid-19 virus).

[0085] See also Figure 2 , Figure 4 , Figure 6 The cleaning container 5 is a tubular container with a screw cap. Similar to the cracking container 3, the cleaning container 5 has a rim 51 on the outside of the bottom and a recess 52 on the inside. The cleaning container 5 is filled with a cleaning liquid 53. The cleaning liquid 53 can be ethanol with a concentration of 60 to 90 wt% (wherein 60 to 90 wt% includes 60 wt%, 61 wt%, 62 wt%, 63 wt%, 64 wt%, 65 wt%, 66 wt%). %, 67wt%, 68wt%, 69wt%, 70wt%, 71wt%, 72wt%, 73wt%, 74wt%, 75wt%, 76wt%, 77wt%, 78wt%, 79wt%, 80wt%, 81wt%, 82wt%, 83wt%, 84wt%, 85wt%, 86wt%, 87wt%, 88wt%, 89wt%, 90wt%), and the concentrations may be the same or different.

[0086] In the packaging state, the cleaning containers 5 are all placed flat in the cleaning operation groove 13, and the bottom surface of the cleaning operation groove 13 is concavely provided with a cleaning container fixing groove 131, and the outer diameter of the cleaning container fixing groove 131 is matched with the outer diameter of the cleaning container 5. Therefore, when the cleaning operation is implemented, the cleaning operation needs to be performed twice, and the cleaning containers 5 can be respectively inserted upright into the cleaning container fixing groove 131, and the rim 51 is snapped on the bottom surface of the cleaning container fixing groove 131.

[0087] See also Figure 2 , Figure 4The lysis operation slot 12 and the cleaning operation slot 13 are adjacently formed on the same surface of the working base 1, and the lysis container fixing slot 121 and the cleaning container fixing slot 131 are staggered one after the other, that is, the lysis container fixing slot 121 is located at the front end of the lysis operation slot 12, the cleaning container fixing slot 131 adjacent to the lysis container fixing slot 121 is located at the rear end of the corresponding cleaning operation slot 13, and the other cleaning container fixing slot 131 is located at the front end of the corresponding cleaning operation slot 13, thereby staggering the placement positions of the various containers to avoid collision or interference during operation.

[0088] See also Figure 7 The absorber 6 comprises a magnetic member 61 and a casing 62 which is sleeved outside the magnetic member 61 . One end of the magnetic member 61 comprises a magnetic head 611 . The casing 62 is a long shell with one end closed and the other end open.

[0089] See also Figure 2 , Figure 7 to Figure 9 When the lysis container 3 or the cleaning container 5 contains a plurality of the magnetic beads 4, the adsorber 6 can extend into the lysis container 3 or the cleaning container 5, and be attracted by the magnetic force of the magnetic head 611, and the magnetic beads 4 will adhere to the outer periphery of the casing 62 corresponding to the outer periphery of the magnetic head 611, and the adsorber 6 can be used to take the magnetic beads 4 out of the lysis container 3 or the cleaning container 5 separately. If the magnetic attraction member 61 is taken out of the casing 62 separately, the magnetic beads 4 are no longer attracted by the magnetic force, and the magnetic beads 4 can be separated from the outer periphery of the casing 62, so that the magnetic beads 4 are placed in a predetermined position to facilitate purification or cleaning operations, as well as detection operations.

[0090] See also Figure 2 , Fig.10 The static groove 15 has a magnetic attraction portion 151, which can be a convex structure. After completing the secondary purification or cleaning operation, the cleaning container 5 needs to be static. Therefore, the cleaning container 5 that needs to be static is upright inserted into the static groove 15, and the rim 51 is snapped on the bottom surface of the static groove 15, and the recess 52 can correspond to and adhere to the magnetic attraction portion 151. Through the magnetic attraction force of the magnetic attraction portion 151, especially the convex structure, the magnetic beads 4 placed in the cleaning container 5 can be effectively adsorbed and gathered in the recess 52.

[0091] See also Figure 2 , Figure 4 , Fig.11The calibration liquid container 9 is a screw-capped tubular container. Similar to the lysis container 3, the bottom of the calibration liquid container 9 has a rim 91. The calibration liquid container 9 is filled with calibration liquid 92. In addition, the dropper 8 has a large suction groove 81 and a small suction groove 82. The dropper 8 can be used to suck the calibration liquid 92 and drip it into the detection port of the sensor 7. When in use, the large suction groove 81 can be pressed to suck the calibration liquid 92, and the small suction groove 82 can be pressed to drip the calibration liquid into the sensor 7, so that the amount of dripping can be controlled not to be too much.

[0092] In the packaging state, the calibration fluid container 9 is placed flat in the calibration operation groove 19, and the bottom surface of the calibration operation groove 19 is concavely provided with a calibration fluid container fixing groove 191, and the outer diameter of the calibration fluid container fixing groove 191 matches the outer diameter of the calibration fluid container 9. Therefore, in the calibration operation state, the calibration fluid container 9 can be uprightly inserted into the calibration fluid container fixing groove 191, and the rim 91 is engaged with the bottom surface of the calibration fluid container fixing groove 191.

[0093] The above-mentioned microorganism detection device can be used to implement the nucleic acid detection method proposed in the present invention. Combined with the operating instructions of the method, the detailed usage of the microorganism detection device of the present invention can be more completely understood. The method of the present invention is demonstrated and explained in the following embodiments, but the present invention is not limited to the following embodiments.

[0094] The present invention provides a detection method using a microorganism detection device, the steps of the method are as follows:

[0095] A. Please also refer to Figure 2 , Figures 5 to 10 Take the sampling stick 2, and use the cotton part of the sampling stick 2 to collect the biological sample, and the biological sample can be the oral or nasal mucosa of the collection target;

[0096] B. Take the lysis container 3 filled with the lysis solution 33, insert the lysis container 3 upright on the working base 1, and place the cotton part of the sampling stick 2 into the lysis container 3, so that the collected biological sample is immersed in the lysis solution 33 for 10 minutes. The lysis solution 33 is mixed with a plurality of magnetic beads 4, and the surface of the magnetic beads 4 has an adsorption layer 41, and the main components of the lysis solution 33 are ethylenediaminetetraacetic acid (EDTA) (0.1wt%-1.5wt%, including 0.1wt%, 0.2wt%, 0.3wt%, 0.4wt%, 0.5wt%, 0.6wt%, 0.7wt%, 0.8wt%, 0.9wt%, 1wt%, 1.1wt%, 1.2wt%, 1.3wt%, 1.4wt%, 1.5wt%) and sodium dodecyl sulfate (Sodium dodecyl sulfate sulfate) (0.05wt%-2wt%, including 0.05wt%, 0.1wt%, 0.2wt%, 0.3wt%, 0.4wt%, 0.5wt%, 0.6wt%, 0.7wt%, 0.8wt%, 0.9wt%, 1wt%, 1.1wt%, 1.2wt%, 1.3wt%, 1.4wt%, 1.5wt%, 1.6wt%, 1.7wt%, 1.8wt%, 1.9wt%, 2wt%);

[0097] C. The lysis solution 33 acts on the biological sample to lyse the cells and viruses in the biological sample to obtain the nucleic acids. After the nucleic acids are released, they will be adsorbed by the adsorption layer 41 on the surface of the magnetic beads 4;

[0098] D. Take the cleaning container 5 containing the first group of cleaning liquids 53 and insert the cleaning container 5 upright on the working base 1. There are two groups of cleaning liquids 53, and the concentrations of the cleaning liquids 53 are both above 80%, and the concentrations can be the same or different, and the main component of the cleaning liquids 53 is ethanol;

[0099] E. Place the adsorber 6 in the lysis container 3. The magnetic beads 4 adsorbed with nucleic acid are attracted by the magnetic force of the magnetic attraction member 61 and then attached to the outer shell 62 of the magnetic attraction member 61.

[0100] F. Place the adsorber 6 with the magnetic beads 4 in the first group of cleaning containers 5, take out the magnetic attraction member 61 separately, so that the magnetic beads 4 are no longer attracted by the magnetic force, and then fall into the first group of cleaning containers 5, stir at least twenty times with the casing 62, and let the magnetic beads 4 be cleaned in the first group of cleaning liquid 53. After the first cleaning is completed, put the magnetic attraction member 61 again, so that the cleaned magnetic beads 4 are adsorbed on the casing 62 by the magnetic force;

[0101] G. Take a cleaning container 5 filled with the second set of cleaning liquid 53, and insert the cleaning container 5 upright on the working base 1;

[0102] H. Place the adsorber 6 with the magnetic beads 4 adsorbed after the first cleaning into the cleaning container 5 of the second group, take out the magnetic attraction member 61 separately, so that the magnetic beads 4 are no longer attracted by the magnetic force, and then fall into the cleaning container 5 of the second group, use the casing 62 to stir for at least twenty times, and let the magnetic beads 4 be cleaned in the second group of cleaning solution 53 (equivalent to repeating the action of step F). After the second cleaning is completed, let the cleaning container 5 stand for 1 minute in the standing tank 15 with the magnetic attraction part 151, and the magnetic attraction part 151 can attract the magnetic beads 4 to settle to the bottom of the cleaning container 5;

[0103] I. Put the magnetic suction member 61 again, so that the magnetic beads 4 after the second cleaning are adsorbed on the housing 62 by the magnetic force. At this point, the nucleic acid sampling action is completed. At this time, the nucleic acid on the magnetic beads 4 has been cleaned and is suitable for screening purposes. The nucleic acid detection action can be performed later;

[0104] J. Please refer to Figure 2-Figure 3 , Figure 11 to Figure 13 During the test, force is applied at the tearing line 173 to tear the wire receiving slot 172 away from the sensor slot 17, and the power cord 72 located inside the wire receiving slot 172 is taken out. One end of the power cord 72 is inserted into the power socket 71, and the other end is connected to the power plug (not shown). Next, the calibration liquid 92 is sucked up by the dropper 8. The calibration liquid 92 is a salt-free liquid, and the main component of the calibration liquid 92 is generally pure water. A drop of the calibration liquid 92 is dripped into the sensor 7 through the dropper 8 to confirm whether the sensor 7 can be used normally;

[0105] K. Please refer to Fig. 9 , Fig.14 , the adsorber 6 with the magnetic beads 4 adsorbed thereon after the second washing is placed into the sensor 7 containing the calibration solution 92, and the magnetic attraction element 61 is taken out separately so that the magnetic beads 4 are no longer attracted by the magnetic force and then fall into the calibration solution 92. The sensor 7 can then be used to detect whether the magnetic beads 4 are adsorbed with the nucleic acid to be detected.

[0106] From the above operation steps, it can be known that during the detection operation, the sensor 7 does not need to be removed from the working base 1, but is firmly placed flat in the sensor slot 17. Compared with placing the sensor 7 directly on the table or holding it by hand, placing the sensor 7 firmly and flat in the sensor slot 17 can make the sensor 7 less likely to shake or be bumped during detection, and can avoid affecting the detection result due to shaking or collision. Similarly, the power cord 72 is also not easy to shake or be bumped, and it can also avoid the power cord 72 from shaking or bumping to cause poor circuit contact. Overall, the effect of improving the stability of the detection result is achieved.

[0107] The above-mentioned embodiments are only some of the preferred embodiments of the present invention, but they are not used to limit the present invention. Any person familiar with this technical field with ordinary knowledge, after understanding the above-mentioned technical features and embodiments of the present invention, and making equal changes or modifications without departing from the spirit and scope of the present invention, still falls within the scope of the present invention, and the patent protection scope of the present invention shall be subject to the definition of the claims attached to this specification.

Claims

1. A microorganism detection device, characterized in that: Include: A working base, comprising a sampling component slot, a sensor slot, a dropper slot and a calibration liquid container slot, wherein the sensor slot has a wire operating slot on one side adjacent to the edge of the working base, the wire operating slot communicates the sensor slot with the outside of the working base, the bottom of the sensor slot has a detachable wire accommodating slot, the outer diameter of the wire accommodating slot is smaller than the outer diameter of the sensor slot, the wire accommodating slot has a tearing line on the slot wall close to the sensor slot, the tearing line is used to facilitate tearing the wire accommodating slot from the sensor slot, wherein the sampling component slot, the sensor slot, the dropper slot and the calibration liquid container slot are arranged on the same plane of the working base; The sensor has a power socket on one side, the power socket is for inserting a power cord, and the other end of the power cord is connected to a power plug. The sensor is placed flat on the sensor slot, and the power socket corresponds to the wire operating slot, and the power cord is rolled up in the wire accommodating slot; A dropper is placed in the dropper groove, and the dropper is used to absorb the correction fluid; and The calibration fluid container is placed in the calibration fluid container tank, and the calibration fluid container is filled with the calibration fluid.

2. The microorganism detection device according to claim 1, characterized in that: The microorganism detection device also includes a sampling rod, a lysis container, two cleaning containers and an adsorber.

3. The microorganism detection device according to claim 2, characterized in that: The sampling component slot includes a sampling rod slot, a lysis operation slot, two cleaning operation slots, an adsorber slot, a static slot and a vertical slot. The sampling rod is placed in the sampling rod slot, the lysis container is placed in the lysis operation slot, the cleaning containers are placed in the cleaning operation slots respectively, and the cleaning containers are all filled with cleaning liquid. The adsorber is placed in the adsorber slot or uprightly inserted in the vertical slot, and the adsorber has a magnetic suction component and a shell mounted on the outside of the magnetic suction component.

4. The microorganism detection device according to claim 2, characterized in that: The lysis container is filled with lysis solution and a plurality of magnetic beads, and the surface of the magnetic beads has an adsorption layer for adsorbing nucleic acids contained in the biological sample.

5. The microorganism detection device according to claim 2, characterized in that: The bottom surface of the cracking operation tank is concavely provided with a cracking container fixing groove, and the outer diameter of the cracking container matches the outer diameter of the cracking container fixing groove, so the cracking container can be vertically inserted into the cracking container fixing groove.

6. The microorganism detection device according to claim 3, characterized in that: The bottom surface of the cleaning operation tank is respectively recessed with a cleaning container fixing groove, the static groove has a magnetic attraction portion, the bottom end of the cleaning container has an outer rim and an inner recess, the outer diameter of the cleaning container is matched with the outer diameter of the cleaning container fixing groove or the static groove, so the cleaning container can be uprightly inserted into the cleaning container fixing groove or the static groove, and the rim is snapped onto the bottom surface of the cleaning container fixing groove or the static groove, and when the cleaning container is inserted into the static groove, the recess can correspond to and adhere to the magnetic attraction portion.

7. The microorganism detection device according to claim 1, characterized in that: The bottom end of the correction fluid container has an outer rim, and the bottom surface of the correction operation groove is concavely provided with a correction fluid container fixing groove. The outer diameter of the correction fluid container fixing groove matches the outer diameter of the correction fluid container, so that the correction fluid container can be inserted into the correction fluid container fixing groove, and the rim is snapped into the bottom surface of the correction fluid container fixing groove.

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