Immune polypeptide freeze-dried powder for pets and preparation method thereof

Abstract

The invention provides immune polypeptide freeze-dried powder for pets and a preparation method thereof, the immune polypeptide freeze-dried powder for pets comprises the following raw materials: animal spleen, compound probiotics and plant extract, and the animal spleen comprises cattle spleen and pig spleen. The immune polypeptide freeze-dried powder is used for various animal spleens, improves the content of spleen aminopeptide, enriches immune factors, and can supplement and stimulate the immunity of the body in time and recover the state of the body.

Description

Technical Field

[0001] The invention belongs to the technical field of pet products, and in particular relates to a freeze-dried immune polypeptide powder for pets and a preparation method thereof. Background Art

[0002] Spleen aminopeptide freeze-dried powder is a polypeptide amino acid and polynucleotide extracted from the spleen of healthy animals, which has the effect of improving immunity. Spleen aminopeptide freeze-dried powder is mainly used to treat diseases such as low cellular immune function, immunodeficiency and autoimmune dysfunction, and has a certain preventive and therapeutic effect on respiratory tract infections, bronchitis, pneumonia, and bronchial asthma of the respiratory system. Invention patent CN118370354A discloses a nutritional supplement for pet kittens, including lactoferrin, fructooligosaccharide, mannooligosaccharide, inulin, yam extract, vitamin C, freeze-dried bovine spleen polypeptide, and probiotics. Since the spleen polypeptide only includes bovine spleen polypeptide, the immune factor is single and the effect of improving immunity is weak. Summary of the invention

[0003] In view of the shortcomings of the prior art, the present invention provides a freeze-dried immune polypeptide powder for pets and a preparation method thereof. The freeze-dried immune polypeptide powder uses spleens of various animals to increase the spleen aminopeptide content, enrich immune factors, and can timely supplement and stimulate the body's immune ability and restore the body's state.

[0004] In order to achieve the above object, the present invention adopts the following technical solutions:

[0005] In one aspect, the present invention provides a freeze-dried immune polypeptide powder for pets, comprising the following raw materials: animal spleen, compound probiotics and plant extracts, wherein the animal spleen comprises bovine spleen and porcine spleen.

[0006] Preferably, the weight fraction of the animal spleen is 90 parts, the weight fraction of the composite probiotics is 1-5 parts, and the weight fraction of the plant extract is 6-16 parts.

[0007] Preferably, the mass ratio of the pig spleen to the bovine spleen is (3:7)-(7:3).

[0008] Preferably, the composite probiotics include at least one of Lactobacillus reuteri, Bifidobacterium animalis, and Lactobacillus plantarum.

[0009] Preferably, the composite probiotics include Lactobacillus reuteri, Bifidobacterium animalis and Lactobacillus plantarum; the mass ratio of Lactobacillus reuteri, Bifidobacterium animalis and Lactobacillus plantarum is 5:5:(2.5-5).

[0010] Preferably, the plant extract comprises at least one of chicory fiber and licorice flavonoids.

[0011] Preferably, the plant extract comprises chicory fiber and glycyrrhizin; the chicory fiber comprises 5-15 parts and the glycyrrhizin comprises 1 part.

[0012] On the other hand, the present invention provides a method for preparing the above-mentioned pet immune polypeptide freeze-dried powder, comprising the following steps:

[0013] S1, sequentially homogenizing, enzymolyzing and ultrafiltering the spleen of the animal to obtain spleen aminopeptide concentrate;

[0014] S2. The spleen amino peptide concentrate, compound probiotics and the plant extract are sequentially mixed, sterilized, freeze-dried and crushed to obtain freeze-dried immune polypeptide powder for pets.

[0015] Preferably, in step S1, bromelain is used to enzymolyze the animal spleen, and the enzymolysis temperature is 4-6° C., and the enzymolysis time is not less than 24 hours.

[0016] Preferably, in step S2, the plant extract includes glycyrrhiza flavonoids, and the preparation steps of the glycyrrhiza flavonoids are: dry and crush the licorice rhizomes, soak them with 95% ethanol to obtain an extract, filter the extract to obtain a filtrate, reduce the pressure and concentrate the filtrate into a thick paste, add hot water and stir to dissolve, and extract with petroleum ether and ethyl acetate in turn to obtain brown-yellow glycyrrhiza flavonoids.

[0017] Compared with the prior art, the present invention has the following beneficial effects:

[0018] (1) The immune polypeptide freeze-dried powder of the present invention uses the spleen of cattle and pigs, which increases the spleen aminopeptide content and enriches the immune factors, and can timely supplement and stimulate the body's immune ability and restore the body's condition.

[0019] (2) The present invention uses live probiotics, which significantly enhance the digestion-promoting ability and immune-enhancing effect of the intestines compared to postbiotics, and effectively proliferates to continuously protect the intestines. The added chicory fiber promotes digestion and can effectively protect the bacteria during the freeze-drying process. Licorice flavonoids can regulate serum biochemical enzyme activity, improve antioxidant capacity, relieve inflammation of immune organs, and effectively enhance the effect of spleen aminopeptides.

[0020] (3) The present invention adopts a low temperature of 4°C and a long enzymatic hydrolysis process of not less than 24 hours, thereby improving the preservation effect of spleen aminopeptide. DETAILED DESCRIPTION

[0021] The present invention is further described in detail below in conjunction with specific embodiments so that those skilled in the art can understand the present invention more clearly.

[0022] In the following examples, Lactobacillus plantarum Lp90, Bifidobacterium animalis BLa80 and Lactobacillus reuteri LR08 were purchased from Weikang Probiotics (Suzhou) Co., Ltd.

[0023] Bromelain (Model: B832358, from: Shanghai MacLean Biochemical Technology Co., Ltd.).

[0024] Example 1

[0025] This embodiment provides a freeze-dried immune polypeptide powder for pets, including 90 parts of animal spleen, 1 part of compound probiotics, 15 parts of chicory fiber and 1 part of licorice flavonoids. The animal spleen is composed of pig spleen and bovine spleen in a mass ratio of 7:3, and the compound probiotics are composed of Lactobacillus reuteri, Bifidobacterium animalis and Lactobacillus plantarum in a mass ratio of 5:5:5.

[0026] The preparation method of pet immune polypeptide freeze-dried powder comprises the following steps: first, fresh spleen is washed and the fascia part is removed, homogenized by using a colloid mill, and bromelain is added to perform enzymatic hydrolysis at 4°C for 24 hours, and ultrafiltration is performed after enzymatic hydrolysis to obtain spleen amino peptide concentrate. The spleen amino peptide concentrate, compound probiotics and plant extract are evenly mixed, sterilized, freeze-dried and crushed to obtain the pet immune polypeptide freeze-dried powder.

[0027] The preparation steps of the licorice flavonoids are as follows: dry and crush the licorice rhizome at room temperature (24-26°C) (crushed to particles that can pass through a 60-mesh sieve), soak it in 95% ethanol at room temperature for 48 hours, filter the extract, and concentrate the filtrate in a 70°C water bath under reduced pressure to form a thick paste. After stirring and dissolving with hot water, extract the solution with petroleum ether, and then extract it with ethyl acetate. The ethyl acetate extract is diluted with 5% Na 2 CO 3 Aqueous solution extraction. After extraction, the solution is adjusted to pH 5-6 with concentrated hydrochloric acid, and then extracted with ethyl acetate. The ethyl acetate is concentrated under reduced pressure, and the concentrate is dried in a 60°C constant temperature oven to constant weight to obtain brown-yellow licorice flavonoids.

[0028] Example 2

[0029] Provided are a pet immune polypeptide freeze-dried powder and a preparation method thereof, which are the same as those in Example 1, except that the weight portion of the chicory fiber is 10 portions.

[0030] Example 3

[0031] Provided are a pet immune polypeptide freeze-dried powder and a preparation method thereof, which are the same as those in Example 1, except that the weight portion of the chicory fiber is 5 portions.

[0032] Example 4

[0033] Provided are a pet immune polypeptide freeze-dried powder and a preparation method thereof, which are the same as Example 2, except that the composite probiotics are composed of Lactobacillus reuteri, Bifidobacterium animalis, and Lactobacillus plantarum in a mass ratio of 5:5:3.

[0034] Example 5

[0035] Provided are a pet immune polypeptide freeze-dried powder and a preparation method thereof, which are the same as Example 2, except that the composite probiotics are composed of Lactobacillus reuteri, Bifidobacterium animalis, and Lactobacillus plantarum in a mass ratio of 5:5:2.5.

[0036] Example 6

[0037] Provided are a freeze-dried immune polypeptide powder for pets and a preparation method thereof, which are the same as those in Example 2, except that the animal spleen is composed of pig spleen and bovine spleen in a mass ratio of 5:5.

[0038] Example 7

[0039] Provided are a pet immune polypeptide freeze-dried powder and a preparation method thereof, which are the same as Example 6, except that the composite probiotics are composed of Lactobacillus reuteri, Bifidobacterium animalis, and Lactobacillus plantarum in a mass ratio of 5:5:3.

[0040] Example 8

[0041] Provided are a pet immune polypeptide freeze-dried powder and a preparation method thereof, which are the same as Example 6, except that the composite probiotics are composed of Lactobacillus reuteri, Bifidobacterium animalis, and Lactobacillus plantarum in a mass ratio of 5:5:2.5.

[0042] Example 9

[0043] Provided are a freeze-dried immune polypeptide powder for pets and a preparation method thereof, which are the same as those in Example 2, except that the animal spleen is composed of pig spleen and bovine spleen in a mass ratio of 3:7.

[0044] Example 10

[0045] Provided are a pet immune polypeptide freeze-dried powder and a preparation method thereof, which are the same as Example 9, except that the composite probiotics are composed of Lactobacillus reuteri, Bifidobacterium animalis, and Lactobacillus plantarum in a mass ratio of 5:5:3.

[0046] Embodiment 11

[0047] Provided are a pet immune polypeptide freeze-dried powder and a preparation method thereof, which are the same as Example 9, except that the composite probiotics are composed of Lactobacillus reuteri, Bifidobacterium animalis, and Lactobacillus plantarum in a mass ratio of 5:5:2.5.

[0048] Comparative Example 1

[0049] Provided is a freeze-dried immune polypeptide powder for pets and a preparation method thereof, which is the same as Example 2, except that the animal spleen is a pig spleen.

[0050] Comparative Example 2

[0051] Provided is a freeze-dried immune polypeptide powder for pets and a preparation method thereof, which is the same as Example 2, except that the animal spleen is a bovine spleen.

[0052] Comparative Example 3

[0053] Provided is a pet immune polypeptide freeze-dried powder and a preparation method thereof, which is the same as Example 2, except that no composite probiotics are included and no composite probiotics are added during the preparation process.

[0054] Comparative Example 4

[0055] Provided is a freeze-dried immune polypeptide powder for pets and a preparation method thereof, which is the same as Example 2, except that chicory fiber is removed.

[0056] Comparative Example 5

[0057] Provided is a pet immune polypeptide freeze-dried powder and a preparation method thereof, which is the same as Example 2, except that glycyrrhizin is removed.

[0058] Comparative Example 6

[0059] Provided are a pet immune polypeptide freeze-dried powder and a preparation method thereof, which are the same as those in Example 2, except that the weight portion of the composite probiotic is 1, and the composite probiotic is Lactobacillus reuteri.

[0060] Comparative Example 7

[0061] Provided is a pet immune polypeptide freeze-dried powder and a preparation method thereof, which is the same as Example 2, except that the weight portion of the composite probiotic is 1, and the composite probiotic is animal Bifidobacterium.

[0062] Comparative Example 8

[0063] Provided are a pet immune polypeptide freeze-dried powder and a preparation method thereof, which are the same as those in Example 2, except that the weight portion of the composite probiotic is 1, and the composite probiotic is Lactobacillus plantarum.

[0064] Comparative Example 9

[0065] Provided are a pet immune polypeptide freeze-dried powder and a preparation method thereof, which are the same as Example 2, except that the weight portion of the composite probiotic is 1, the composite probiotic includes Lactobacillus reuteri and Bifidobacterium animalis, and the mass ratio of Lactobacillus reuteri to Bifidobacterium animalis is 1:1.

[0066] Comparative Example 10

[0067] Provided are a pet immune polypeptide freeze-dried powder and a preparation method thereof, which are the same as those in Example 2, except that the weight portion of the composite probiotic is 1, the composite probiotic includes Lactobacillus reuteri and Lactobacillus plantarum, and the mass ratio of Lactobacillus reuteri to Lactobacillus plantarum is 1:1.

[0068] Comparative Example 11

[0069] Provided are a pet immune polypeptide freeze-dried powder and a preparation method thereof, which are the same as those in Example 2, except that the weight portion of the composite probiotic is 1, the composite probiotic includes animal bifidobacterium and plant lactobacillus, and the mass ratio of animal bifidobacterium to plant lactobacillus is 1:1.

[0070] Performance testing and results

[0071] The experimental dogs in different groups were fed 2g / kg according to their body weight. On the 15th day, blood was collected from the medial saphenous vein of the forelimbs of 25 dogs before feeding (blood volume 4-6ml / time / dog). The blood was collected in ordinary negative pressure blood collection tubes and centrifuged at 5000r / min for 10min to separate the serum. The upper layer of serum was transferred to each reagent cup for biochemical index detection, mainly detecting the contents of IgG, lgM, IgA and complement C3 and C4. Table 1 shows the test results.

[0072] Table 1 Pet immunity test results on the 15th day

[0073]

[0074]

[0075] As can be seen from Table 1, in the same period of time, the improvement of the immune ability of the pet immune polypeptide freeze-dried powder prepared in Examples 1-11 is significantly higher than that of Comparative Examples 1-11, which shows that the formula of pig spleen, bovine spleen, composite probiotics, chicory fiber, and licorice flavonoids in the present invention can significantly improve immunity. Therefore, the composition of the pet immune polypeptide freeze-dried powder in Example 2 is selected as the best formula in the present invention.

[0076] Other raw materials or structures not specifically described in the present invention already exist in the prior art and can be directly purchased from the market.

[0077] The above are only preferred embodiments of the present invention and are not intended to limit the protection scope of the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A freeze-dried immune polypeptide powder for pets, characterized in that: Including the following ingredients: Animal spleens, including bovine and porcine spleens; Probiotic complex; and Plant extracts.

2. The freeze-dried immune polypeptide powder for pets according to claim 1, characterized in that: The weight fraction of the animal spleen is 90 parts, the weight fraction of the composite probiotics is 1-5 parts, and the weight fraction of the plant extract is 6-16 parts.

3. The freeze-dried immune polypeptide powder for pets according to claim 1, characterized in that: The mass ratio of the pig spleen to the bovine spleen is (3:7)-(7:3).

4. The freeze-dried immune polypeptide powder for pets according to claim 1, characterized in that: The composite probiotics include at least one of Lactobacillus reuteri, Bifidobacterium animalis, and Lactobacillus plantarum.

5. The lyophilized immune polypeptide powder for pets according to claim 4, characterized in that: The composite probiotics include Lactobacillus reuteri, Bifidobacterium animalis and Lactobacillus plantarum; The mass ratio of the Lactobacillus reuteri, Bifidobacterium animalis and Lactobacillus plantarum is 5:5:(2.5-5).

6. The lyophilized immune polypeptide powder for pets according to claim 1, characterized in that: The plant extract comprises at least one of chicory fiber and licorice flavonoids.

7. The lyophilized immune polypeptide powder for pets according to claim 2, characterized in that: The plant extracts include chicory fiber and licorice flavonoids; The chicory fiber is 5-15 parts and the liquorice flavonoids is 1 part.

8. The method for preparing the freeze-dried immune polypeptide powder for pets according to any one of claims 1 to 7, characterized in that: The following steps are involved: S1, sequentially homogenizing, enzymolyzing and ultrafiltering the spleen of the animal to obtain spleen aminopeptide concentrate; S2. The spleen amino peptide concentrate, compound probiotics and the plant extract are sequentially mixed, sterilized, freeze-dried and crushed to obtain freeze-dried immune polypeptide powder for pets.

9. The method according to claim 8, characterized in that In step S1, the animal spleen is enzymatically hydrolyzed using bromelain, and the enzymatic hydrolysis temperature is 4-6° C., and the enzymatic hydrolysis time is not less than 24 hours.

10. The method according to claim 8, characterized in that In step S2, the plant extract includes glycyrrhiza flavonoids, and the preparation steps of the glycyrrhiza flavonoids are as follows: dry and crush the licorice rhizomes, soak them with 95% ethanol to obtain an extract, filter the extract to obtain a filtrate, reduce the pressure and concentrate the filtrate into a thick paste, add hot water and stir to dissolve, and then extract with petroleum ether and ethyl acetate in turn to obtain brown-yellow glycyrrhiza flavonoids.

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